PI: Takaomi Saido
Affiliation: RIKEN Brain Science Institute
Submitted by: Nobuhisa Iwata
Posted Date: 24 Feb 2009

Brief Description

This is a protocol for the immunostaining of neprilysin using a high temperature antigen unmasking technique and the immunoperoxidase-indirect or fluorescence-indirect tyramide signal amplification (TSA) method.

References

1. Iwata N, Takaki Y, Fukami S, Tsubuki S, Saido TC. Region-specific reduction of A beta-degrading endopeptidase, neprilysin, in mouse hippocampus upon aging. J Neurosci Res. 2002 Nov 1;70(3):493-500. PubMed.
2. Fukami S, Watanabe K, Iwata N, Haraoka J, Lu B, Gerard NP, Gerard C, Fraser P, Westaway D, St George-Hyslop P, Saido TC. Abeta-degrading endopeptidase, neprilysin, in mouse brain: synaptic and axonal localization inversely correlating with Abeta pathology. Neurosci Res. 2002 May;43(1):39-56. PubMed.
3. Iwata N, Mizukami H, Shirotani K, Takaki Y, Muramatsu S, Lu B, Gerard NP, Gerard C, Ozawa K, Saido TC. Presynaptic localization of neprilysin contributes to efficient clearance of amyloid-beta peptide in mouse brain. J Neurosci. 2004 Jan 28;24(4):991-8. PubMed.

Keywords

Aβ-degrading, Immunofluorescence, DAB

Further Reading

No Available Further Reading