Pronuclear Injection for the Production of Transgenic Mice
PI: Jürgen Götz
Affiliation: University of Sydney
Submitted by: Jürgen Götz
Posted Date: 13 Apr 2009
Pronuclear injection is the most widely used protocol for the generation of transgenic mice. Here, we describe all steps involved from DNA purification to the set up of a mouse colony including vasectomy, injection of the DNA into a donor zygote, transfer of injected zygotes into recipient foster mice, screening of offspring and establishment of transgenic mouse lines. We discuss the use of neuron-specific promoters to express proteins with a role in Alzheimer disease. Transgenic expression of a truncated form of the microtubule-associated protein tau (Dtau) is used as an example for the anticipated results. This protocol is available through Nature Protocols.
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mouse model, transgenic
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