A protocol for the efficient transfection of cultured primary motor neurons via magnetofection, a transfection technology based on the delivery of DNA-coated magnetic nanobeads. This protocol uses the MF10000 Super Magnetic Plate or MF14000 Mega Magnetic Plate (Oz Biosciences) and NM51000 NeuroMag transfection reagent (Oz Biosciences).
- Fallini C, Bassell GJ, Rossoll W. High-efficiency transfection of cultured primary motor neurons to study protein localization, trafficking, and function. Mol Neurodegener. 2010 Apr 21;5:17. PubMed.
transfection, motor neurons, primary neurons, mouse