331395 RESULTS
PROTOCOL A protocol for the efficient transfection of cultured primary motor neurons via magnetofection, a transfection technology based on the delivery of DNA-coated magnetic nanobeads. This protocol uses the MF10000 Super Magnetic Plate or MF14000 Mega Magnetic
PROTOCOL This protocol includes procedures for the collection, processing and shipment of clinical laboratory and APOE samples, cell immortalization samples at baseline, blood and urine samples for biomarkers, and cerebral spinal fluid samples. For more informatio
PROTOCOL An overview of how to collect CSF by lumbar puncture and process it for future analysis. fagana Washington University School of Medicine CSF, cerebrospinal fluid, lumbar puncture, biomarker protocol_CSF_sample_collection.pdf Collection/Processing of Biofl
PROTOCOL The objective of the QC program is to standardize CSF biomarker measurements between labs. This protocol is a standardized protocol for lumbar puncture and CSF sample processing.Notes:For the CSF processing and analysis part, it is recommended that the cl
PROTOCOL This protocol uses the Bio-Rad blotting system. dselkoe Harvard Medical School and the Brigham and Women's Hospital Western blot, PVDF, Transfer buffer protocol_Western_blotting.pdf Western Blotting
PROTOCOL Frequently used on human and mouse brain sections for detecting pathologies in the endocytic-autophagic-lysosomal pathways such as changes in the numbers, types and distribution of vacuoles, and expression levels of individual molecules in the pathways. n
PROTOCOL Uptake of HRP into neuronal cells occurs via bulk endocytosis, especially at axonal terminals. Endocytosed HRP is transported in a retrograde direction and delivered into autophagic-lysosomal vacuoles in the cell body and, therefore, can serve as a marker
PROTOCOL In vivo cathepsin activity assay can provide information about the lysosomal enzyme activity in living cells. nixon New York University School of Medicine and the Nathan Kline Institute Probing the cathepsin D using a BODIPY FL-pepstatin A: applications i
PROTOCOL Autophagic-lysosomal compartments contain numerous acid hydrolases. Analysis using enzyme histochemistry for acid phosphatase at the ultrastructural level can provide information about the trafficking and distribution of acid hydrolases in neuronal proces
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PAPER Balci F, Oakeshott S, Shamy JL, El-Khodor BF, Filippov I, Mushlin R, Port R, Connor D, Paintdakhi A, Menalled L, Ramboz S, Howland D, Kwak S, Brunner D
PAPER Wang ZG, Qi RB, Zhu LH, Lu DX
PAPER Leal SS, Cardoso I, Valentine JS, Gomes CM
PAPER Hong HB, Nam IH, Sohn SW, Song KB
PAPER Rafii MS
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