PI: Wilfried Rossoll
Affiliation: Emory University
Submitted by: Wilfried Rossoll
Posted Date: 06 Jun 2010

Brief Description

A protocol for the efficient transfection of cultured primary motor neurons via magnetofection, a transfection technology based on the delivery of DNA-coated magnetic nanobeads. This protocol uses the MF10000 Super Magnetic Plate or MF14000 Mega Magnetic Plate (Oz Biosciences) and NM51000 NeuroMag transfection reagent (Oz Biosciences).

References

1. Fallini C, Bassell GJ, Rossoll W. High-efficiency transfection of cultured primary motor neurons to study protein localization, trafficking, and function. Mol Neurodegener. 2010 Apr 21;5:17. PubMed.

Keywords

transfection, motor neurons, primary neurons, mouse

Further Reading