PI: Ralph Nixon
Affiliation: New York University School of Medicine and the Nathan Kline Institute
Submitted by: Ralph Nixon
Posted Date: 19 Jun 2009

Brief Description

Uptake of HRP into neuronal cells occurs via bulk endocytosis, especially at axonal terminals. Endocytosed HRP is transported in a retrograde direction and delivered into autophagic-lysosomal vacuoles in the cell body and, therefore, can serve as a marker for studying endocytic trafficking to autophagic-lysosomal compartments.

References

1. Broadwell RD, Brightman MW. Horseradish peroxidase: a tool for study of the neuroendocrine cell and other peptide-secreting cells. Methods Enzymol. 1983;103:187-218. PubMed.
2. Broadwell RD, Oliver C, Brightman MW. Neuronal transport of acid hydrolases and peroxidase within the lysosomal system or organelles: involvement of agranular reticulum-like cisterns. J Comp Neurol. 1980 Apr 1;190(3):519-32. PubMed.
3. Yang DS, Lee JH, Nixon RA. Monitoring autophagy in Alzheimer's disease and related neurodegenerative diseases. Methods Enzymol. 2009;453:111-44. PubMed.

Keywords

endocytosis, in vivo, mouse, autophagy

Further Reading

No Available Further Reading