PI: Jürgen Götz
Affiliation: University of Sydney
Submitted by: Jürgen Götz
Posted Date: 13 Apr 2009

Brief Description

This protocol describes how to culture embryonic (E16.5) murine hippocampal neurons using a spatially separated ring of cortical neurons for neurotrophic support. This method allows long-term cultures at a very low cell density, and therefore, the study of single embryo preparations and isolated neurons. This method has been adopted for neurons from the substantia nigra (E16.5) with support from a ring of striatal neurons. This protocol is available through Nature Protocols.

References

1. Fath T, Ke YD, Gunning P, Götz J, Ittner LM. Primary support cultures of hippocampal and substantia nigra neurons. Nat Protoc. 2009;4(1):78-85. PubMed.
2. Ittner LM, Fath T, Ke YD, Bi M, van Eersel J, Li KM, Gunning P, Götz J. Parkinsonism and impaired axonal transport in a mouse model of frontotemporal dementia. Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15997-6002. Epub 2008 Oct 2 PubMed.

Keywords

neuron, primary cell, hippocampal, hippocampus, mouse

Further Reading

No Available Further Reading