Jobs

Research Technician

Employer

Rosalind Franklin University / The Chicago Medical School

Location

Chicago , Illinois

Principal Investigator

Grace Stutzmann

Contact

grace.stutzmann@rosalindfranklin.edu

Description

Position Purpose: Research technician position to support ongoing studies in Alzheimer’s disease, TBI, and neurodegenerative disease using stem cell biology, iPSCS, cell culture models and rodent cell based assays.  Drug discovery and screening assays of novel therapeutics is also a major focus.

Essential functions and duties:

  • Cell culture development and maintenance, including iPSCs and human induced neurons
  • Proficiency with viral vectors, subcloning, and transfection techniques
  • Optical imaging and fluorescence techniques, utilization of calcium imaging approaches and related indicators
  • PCR, and gel electrophoresis
  • Experience with RNA, RNA extraction from mouse/rat brains, and qRT-PCR
  • Western blotting and immunoassays, Immunohistochemistry
  • Familiarity with rodent models, behavior, minor surgical techniques
  • Collect, compile and analyze data.

Additional responsibilities:

  • Mouse husbandry (breeding, weaning, maintenance of colonies, genotyping)
  • Orders laboratory supplies and equipment
  • Oversee the maintenance of lab equipment and environment
  • Other duties of laboratory manager
  • Data presentation
  • Detailed record-keeping and organizational skills are essential
  • Mentoring/training of personnel as needed

Requirements

Required Education & Experience:

  • Bachelor's degree (B.S.) and minimum of two years of wet lab experience
  • Demonstrated mastery of stem cell biology, iPSCs, cell culture techniques and molecular biology approaches
  • Minimum B.S. in neuroscience/biology/molecular biology-related field
  • Effective and clear English reading, writing and verbal communication skills
  • Demonstrated project management skills, including ability to manage competing priorities and excellent time management skills
  • Excellent mentoring and training skills

Required Knowledge, Skills & Abilities:

  • Extensive experience in compound screening in model cells using a variety of platforms and methodologies
  • Extensive knowledge of cell culture techniques, including iPSCs and induced neurons
  • Immunoassays: ELISA, WB,
  • Immunohistochemistry

Minimum Education and Experience:

  • Bachelor's degree in a basic science or equivalent education and minimum of two years of wet laboratory experience in the United States
  • Minimum B.S. in neuroscience/biology/molecular biology or related field
  • Effective and clear English reading, writing and verbal communication skills
  • Demonstrated project management skills, including ability to manage competing priorities and excellent time management skills
  • Excellent interpersonal and communication skills, and the ability to work well with others.