NMDAR2B, C-term
Sigma Aldrich
polyclonal
Rabbit
(lyophilized, NH4HCO3, affinity purified)
-
Human, Mouse, Rat
Immunogen = amino acids 984-1104 at C-terminus of NR2B
NMDAR2B (180kDa). No cross-reaction with NR2A aor NR2C subunits
-
NMDA R2B phos Ser1480
Pierce Thermo Scientific
polyclonal
IgG
Rabbit
(liquid, affinity purified, HEPES, saline, BSA, glycerol)
-
Rat
immunogen = synthetic phosphopeptide corresponding to a portion of rat NMDA NR2B Subunit around serine 1480
detects Phospho-NMDA NR2B Subunit (Ser1480)
-
NR2A
Upstate and Chemicon are Millipore
polyclonal
IgG
Rabbit
(liquid, purified, buffer containing storage buffer, Tris-glycine, NaCl, sodium azide and glycerol)
-
Mouse, Rat
Immunogen = 6His-tagged fusion protein corresponding to amino acids 1265-1464 of mouse NMDA receptor 2A (NR2A)
recognizes NR2A, Mw ~170 kDa
-
GluR1 (C3T)
Upstate and Chemicon are Millipore
monoclonal
IgG
Rabbit
(cultured supernantant in sodium azide)
-
Chimpanzee, Human, Mouse, Rat
Immunogen = peptide (TLPRNS GAGAS) corresp. to aa. 858-868 of mature human GluR1
recognizes GluR1, 106 kDa
-
GluR1 (RH95)
Chemicon and Upstate are Millipore
monoclonal
IgG2a ,κ
Mouse
(liquid, purified, buffer with Tris-glycine, saline, sodium azide)
-
Rat
Immunogen = Thyroglobulin conjugated linear peptide of rat GluR1 designed around the N-terminus
recognizes GluR1 at the N-terminus
-
GluR1
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(lyophilized from PBS, BSA, azide, affinity purified)
-
Equine, Human, Mouse, Rat
Immunogen = KLH-conjugated linear peptide corresponding to human Glutamate receptor 1 at the cytoplasmic domain
detects GluR1, cytoplasmic domain
-
NMDA NR1
Pierce Thermo Scientific
monoclonal
Mouse
(lyophilized, affinity purified)
-
Rat
Immunogen = fusion protein corresponding to amino acids 1-564 of rat NMDA NR1
detects NMDA NR1, ~100 kDa in WB; shows no cross-reactivity with any of the NR2 subunits
-
NMDA NR1
Pierce Thermo Scientific
polyclonal
Rabbit
(lyophilized, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic peptide corresponding to residues (849)R A E P D P K K K A T F R A(862) C of rat NR1 exon 21
detects NMDA NR1, ~120 kDa in WB
-
NMDA NR1
Pierce Thermo Scientific
polyclonal
Rabbit
(lyophilized, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic peptide corresponding to residues (195)N Y E N L D Q L S Y D N K R G P(210) C of Exon 5 of rat NMDA Receptor Type 1
detects NMDA NR1, ~120 kDa in WB
-
NMDAR2B phosphoTyr1472
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(liquid, Hepes, NaCl, BSA, glycerol, azide, affinity purified Ig)
-
Rat
Immunogen = synthetic peptide corresp. to aa surrounding phosphoTyr1472 of rat NMDAR 2B
NMDAR 2B phosphorylated Tyr1472 (~180 kDa)
-
CK-2β (6D5)
Sigma Aldrich
monoclonal
IgG1
Mouse
(liquid, PBS, azide)
-
Bovine, Human, Hamster, Mouse, Rat
immunogen = recombinant human casein kinase 2β (CK2β). (the antibody epitope resides within amino acids 123-127)
recognizes Casein Kinase 2β
-
CK-2α
Pierce Thermo Scientific
monoclonal
Mouse
-
Bovine, Hamster, Mouse, Rat
immunogen = denatured purified human recombinant CK2, alpha subunit
reacts with Casein Kinase 2 alpha-subunit
-
AMPKα, phos Thr172
Abcam
polyclonal
IgG
Rabbit
(liquid, antiserum, affinity purified, glycerol, PBS, saline, azide)
-
Human, Mouse, Rat
immunogen = synthetic phospho-peptidederived from human AMPK alpha 1 around the phosphorylation site of threonine 172 (L-R-phosphoT-S-C)
detects endogenous AMPKα only whenphosphorylated at SDGEFLR(pT)SCGSPNY. The antibody detects both alpha1 and alpha2 isoforms
-
AMPKα, phos Thr172 (40H9)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Human, Monkey, Hamster, Mouse, Rat, S. cerevisiae, Drosophila
immunogen = synthetic phospho-peptide(KLH-coupled) corresp. to residues surroundingThr172 of human AMPKα
detects endogenous AMPKα only whenphosphorylated at threonine 172. The antibody detects bothα-1 and α-2 isoforms of the catalytic subunit, but does notdetect the regulatory β or γ subunits
-
Cathepsin B (173317)
R&D Systems
monoclonal
IgG1
Rat
(lyophilized, PBS, trehalose, protein G purified)
-
Human, Mouse
immunogen = purified, NS0-derived, recombinant mouse cathepsin B, aa 18-339
Cathepsin pro and active forms
-
Cathepsin B
R&D Systems
polyclonal
IgG
Goat
(lyophilized, PBS, trehalose, affinity purified)
-
Human
immunogen = NS0-derived human cathepsin B
cathepsin B
-
Cathepsin B
R&D Systems
polyclonal
IgG
Goat
(lyophilized, PBS, BSA, affinity purified, biotinylated)
-
Human
immunogen = purified, NS0-derived, recombinant human cathepsin B
cathepsin B
-
Cathepsin B
R&D Systems
polyclonal
IgG
Goat
(lyophilized, PBS, trehalose, affinity purified)
-
Mouse
immunogen = purified, NS0-derived, recombinant mouse cathepsin B aa 18-30
cathepsin B
-
Cathepsin B
R&D Systems
polyclonal
IgG
Goat
(lyophilized, PBS, trehalose, affinity purified; biotinylated)
-
Mouse
immunogen = purified, NS0-derived recombinant mouse cathepsin B, aa 18-339
cathepsin B
-
Cathepsin L (204101)
R&D Systems
monoclonal
IgG2A
Rat
(lyophilized, PBS, trehalose, protein G purified)
-
Human, Mouse
immunogen = NS0-derived, recomb. human cathepsin L
cathepsin L
-
Cathepsin L
R&D Systems
polyclonal
IgG
Goat
(lyophilized, PBS, trehalose, affinity purified, filtered)
-
Mouse
immunogen = NS0-derived, recomb. mouse cathepsin L (aa 18-334)
cathepsin L
-
Cathepsin B
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = peptide mapping at the C-terminus of human cathepsin B
cathepsin B
-
Cathepsin B
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified; sc-6493P blocking peptide)
-
Human, Mouse, Rat
immunogen = peptide mapping at the N-terminus of human cathepsin B
cathepsin B
-
Cathepsin B
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified; sc-6491P blocking peptide)
-
Human
immunogen = peptide mapping at the N-terminus of human cathepsin B
cathepsin B
-
Cathepsin B
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
immunogen = amino acids 1-339 representing full length human cathepsin B
Cathepsin B
-
Cathepsin B (CB131)
AbD Serotec
monoclonal
IgG2b
Mouse
(lyophilized, supernatant, azide)
-
Human
immunogen = recombinant human cathepsin B
cathepsin B
-
Cathepsin B
AbD Serotec
polyclonal
IgG
Sheep
(liquid, glycine buffered saline, EACA, benzamidine, EDTA, purified)
-
Human
immunogen = human cathepsin B purified from liver
cathepsin B
-
Cathepsin B
AbD Serotec
polyclonal
Sheep
(liquid, PBS, azide, conj. FITC)
-
Human
immunogen = native, from placenta
cathepsin B
-
Cathepsin B
AbD Serotec
polyclonal
Sheep
(liquid, PBS, azide, purified)
-
Human
immunogen = native, from placenta
cathepsin B
-
Cathepsin B
AbD Serotec
polyclonal
Sheep
(liquid, PBS, azide, glycerol, ZnCl2, MgCl2)
-
Human
immunogen = native, highly pure from placenta
cathepsin B
-
Cathepsin B
AbD Serotec
kit
Unknown Host
-
-
-
cathepsin B
-
Cathepsin B
AbD Serotec
kit
Unknown Host
-
-
-
cathepsin B
-
Cathepsin B (6A221)
US Biological
monoclonal
IgG2b
Mouse
(lyophilized, PBS, azide, supernatant)
-
Human
immunogen = recombinant human cathepsin B
cathepsin B
-
Cathepsin B (6D231)
US Biological
monoclonal
IgG2b
Mouse
(liquid, PBS, thimerosal, affinity purified)
-
Human
immunogen = recombinant human procathepsin B
cathepsin B epitope E P G Y S P (aa 212-217); recognizes mature single or two chain cathepsin B and procathepsin B. Does not crossreact with mature cathepsins H, K, L, S, V, W or procathepsins L, S, W.
-
Cathepsin B
US Biological
polyclonal
IgG
Rabbit
(liquid, azide, purified)
-
Human
immunogen = human liver cathepsin B
Cathepsin B; no cross-reaction with Cathepsin L, Cathespin D or Cathepsin H.
-
Cathepsin B
US Biological
polyclonal
IgG
Rabbit
(liquid, thimerosal, gelatin, affinity purified)
-
Human
immunogen = mature form of purified human liver cathepsin B
Cathepsin B (pro and mature forms)
-
Cathepsin B
US Biological
polyclonal
IgG
Sheep
(liquid, GBS, aszide, EACA, Benzamidine, EDTA, purified)
-
Human
immunogen = purified human liver cathepsin B
cathepsin B
-
Cathepsin B
US Biological
polyclonal
IgG
Rabbit
(liquid, BSA, azide, affinity purified)
-
Human
-
Cathepsin B (precursor and mature forms)
-
Cathepsin B
US Biological
polyclonal
IgG
Rabbit
(liquid, tris-glycine saline, azide, protein A purified)
-
Mouse, Rat
immunogen = recombinant rat pro-Cathepsin B1
recognizes procathepsin B (~40kDa) and mature cathepsin B (25kD, 26kD and 30kD)
-
Cathepsin B
BioVision
polyclonal
Rabbit
(liquid, PBS, gelatin, thimerosal, affinity purified)
-
Human
immunogen = purified cathepsin B from human liver
cathepsin B (pro and mature forms)
-
Cathepsin B
BioVision
kit
Unknown Host
-
-
-
cathepsin B
-
Cathepsin B (CA10)
Calbiochem EMD Biosciences
monoclonal
IgG2a
Mouse
(lyophilized, ammonium bicarbonate buffer, BSA)
-
Bovine, Porcine, Human
immunogen = cathepsin B purified from human liver; epitope within the heavy chain of mature cathepsin B
cathepsin (~43kDa latent and ~31kDa active forms)
-
Cathepsin B
Calbiochem EMD Biosciences
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human
immunogen = mature human cathepsin B purified from liver
Cathepsin B (~43kDa latent and ~31kDa active forms)
-
Cathepsin B
Calbiochem EMD Biosciences
polyclonal
IgG
Rabbit
(liquid, PBS, azide, purified)
-
Human
immunogen = purified cathepsin B from human liver
cathepsin B; not cathepsin D, H or L
-
Cathepsin B
Cell Technology
kit
Unknown Host
(substrate-based assay, cell permeable, fluorogenic, lyophilized)
-
-
-
-
-
Cathepsin B
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human
immunogen = Synthetic peptide corresponding to aa 92-108 of human cathepsin B.
recognizes the precursor(~45kDa) and both mature forms of cathepsin B (~28kDa, 30kDa)
-
Cathepsin B
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(liquid, Tris, azide)
-
Mouse, Rat
immunogen = recomb. rat procathepsin B 1
recognizes procathepsin B (40kDa) and mature cathepsin B (25kDa, 26kDa and 30kDa)
-
Tau (CP27)
Formerly available through Dr. Peter Davies. For updated information, contact antibodies@alzforum.org.
monoclonal
IgG2b
Mouse
-
Human
Human tau 130-150
-
-
Tau (CP9)
Formerly available through Dr. Peter Davies. For updated information, contact antibodies@alzforum.org.
monoclonal
IgG2b
Mouse
-
Human, Mouse, Rat
epitope around phospho Threonine 231
tau phospho. at Thr231
-
Tau (RTA-1)
(Taizo Taniguchi, DirectorBehavioral and Medical Science Research Consortium, Hyogo, Japan)
monoclonal
Mouse
(supernate, gel filtered)
-
-
raised against human tau (epitope = first repeat of MBD, aa 257-274)
binds CHO-R3tau and CHO-R4tau; inhibits PHF formation in human tau, completly blocks in vitro 3RMBD and 4RMBD aggregation
-
Tau (TG-5)
Formerly available through Dr. Peter Davies. For updated information, contact antibodies@alzforum.org.
monoclonal
IgG2b
Mouse
-
Human, Mouse
-
tau
-
Tau phos Ser214 (CP3)
Formerly available through Dr. Peter Davies. For updated information, contact antibodies@alzforum.org.
monoclonal
Mouse
-
Human, Mouse
immunogen = amino acids around phosphorylated Ser214
recognizes human and murine tau phosphorylated at S214
-
Tau phos Ser409 (PG5)
Formerly available through Dr. Peter Davies. For updated information, contact antibodies@alzforum.org.
monoclonal
IgG3
Mouse
-
Human, Mouse
immunogen = the synthetic phosphopeptides KSPRHLS(P)NV S(P)STGS(P)ID and KSPRHLS(P)NV SS(P)TGSID
recognizes the PKA-dependent phosphorylation of ser409 in tau; immunoreactivity with neurofibrillary pathology in both early and advanced Alzheimer’s disease, but not in normal brain tissue; reacts with only PHF and the tau peptide containing phospho ser409
-
Tau phos Thr231 (2E12)
Formerly available through Dr. Peter Davies. For updated information, contact antibodies@alzforum.org.
monoclonal
Mouse
-
Human, Mouse
epitope around phospho Threonine 231
highly specific for tau phospho. at Thr231
-
Tau phos Ser235 (MC6)
MC6(Gregory Jicha, Departments of Pathology and Neurpscience, Albert Einstein College of Medicine)References:Vingtdeux, 2010Planel, 2008
monoclonal
Mouse
-
Human
immunogen = affinity purified PHF (epitope around phosphor. Ser235)
recognizes tau phosphor. at Ser235; not reactive to non-phosphor. tau.paper Jincha, et al. 1997
-
Tau, truncated at Asp421 (C3)
TauC3(Vincent L. Cryns, Cell Death Regulation Laboratory, Division of Endocrinology,Metabolism, and Molecular Medicine, Northwestern University, Chicago, IL)Research paperReference: Alonso, 2010
monoclonal
IgG1
Mouse
(purified IgG, HEPES, saline, glycerol.)
-
Human
immunogen = KLH- C S S T G S I D M V D, corresp. to residues 412-421 at the C-terminus of tau truncated at Asp421
recognizes tau when truncated at Asp421; no reaction with full-length tau, or with tau truncated at Glu391 or Ala429
-
Tau (Tau-1)
Tau-1(L.I. Binder, Northwestern Univ.)PaperReference: Alonso, 2010
monoclonal
IgG2a
Mouse
-
Mouse, Rat
detects Tau that is not phosphorylized at at Ser198/Ser199/Ser202
-
Tau410
134d(Inge Grundke-Iqbal, Department of Neurochemistry, New YorkState Institute for Basic Research in Developmental Disabilities)research PaperReference: Alonso, 2010
polyclonal
Rabbit
(antiserum)
-
Human
immunogen = recombinant human Tau410
recognizes tau in a phosphorylation independent manner
-
Tau
111e(Khalid Iqbal, Department of Neurochemistry, New YorkState Institute for Basic Research in Developmental Disabilities)research PaperReference: Alonso, 2010
polyclonal
Rabbit
(antiserum)
-
Human
-
recognizes total tau and hyperphosphorylated tau from AD brain; no cross with bovine tau
-
Tau, total
92e(Inge Grundke-Iqbal, Department of Neurochemistry, New YorkState Institute for Basic Research in Developmental Disabilities)research PaperReference: Alonso, 2010
polyclonal
Rabbit
(antiserum)
-
Human
-
recognizes both phosphorylated and non phosphorylated tau
-
p62 protein
Affiniti - Biomol
polyclonal
IgM,κ
Rabbit
(liquid, antiserum)
-
Human
immunogen = a peptide derived from residues 387-436, comprising the UBA domain of p62
detects human 62 kD (p62) protein, found in various neurodegenerative conditions, including tauopathies (Alzheimer’s disease, Picks disease, and frontotemporal dementia) and synucleinopathies (Parkinson’s disease, dementia with Lewy body disease and multiple system atrophy)
-
Tau, phospho 181P
Innogenetics
kit
Unknown Host
(solid-phase ELISA, uses monoclonal HT7 for capture, AT270 biotinylated for incubation, streptavidin; working range: 25 to 150 pg/mL)
-
Human
specific for tau phosphorylated at threonine 181, antigen in cerebrospinal fluid (CSF)
-
Tau pS396
Biosource Invitrogen
polyclonal
Rabbit
(affinity purified Ig, Dulbecco's PBS, BSA, glycerol, unconjugated)
-
Human
chemically synthesized phosphopeptide derived from theregion of human Tau that contains serine 396.
detects tau phosphorylated at serine 396; no cross with non phosphorylated tau
-
Tau pS356
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, Dulbecco’s PBS, BSA, azide, glycerol, unconjugated)
-
Human
immunogen = chemically synthesized phosphopeptide derived from the region of human Tau that contains serine 396
tau phosphorylated at serine 396
-
Tau pS199/202
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated)
-
Human
tau-derived peptide that is phospho. at serines 199 and 202
tau phosphorylated at serines 199 and 202
-
Tau pS199
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated)
-
Human
immunogen = a chemically synthesized phosphopeptide derived from the region ofhuman Tau that contains serine 199
tau phosphorylated at serine 199
-
Tau pS202
Biosource Invitrogen
polyclonal
Rabbit
(affinity purified Ig)
-
Human
immunogen = human tau-derived synthetic peptide that is phosphorylated at serine 202
tau phosphorylated at serine 202
-
Tau pS262
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol)
-
Human
human tau-derived peptide that is phosphorylated at serine 262
tau phosphorylated at serine 262
-
Tau pS409
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated)
-
Human
human tau-derived peptide that is phosphorylated at serine 409
tau phosphorylated at serine 409
-
Tau pT212
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated)
-
Human
human tau-derived peptide that is phosphorylated at threonine 212
tau, tau phosphor. at threonine 212
-
Tau pT217
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated)
-
Human
human tau-derived peptide that is phosphorylated at threonine 217
tau phosphorylated at threonine 217
-
Tau pT231
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated)
-
Human
human tau-derived peptide that is phosphorylated at threonine 231
tau phosphorylated at threonine 231
-
Tau pS404
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated)
-
Human
human tau-derived peptide that is phosphorylated at serine 404
tau phosphorylated at serine 404
-
Tau pS400
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated)
-
Human
human tau-derived peptide that is phosphorylated at serine 400
tau phosphorylated at serine 400
-
Tau TAUY5
Biosource Invitrogen
polyclonal
Rabbit
(liquid, purified Ig, PBS, azide)
-
Human
immunogen = synthetic peptide, corresp. to aa residues 186-201 of human tau protein isoform 6 (GEPPKSGDRSG-pY-SSPG), conj. to KLH via an N-terminal cysteine residue
detects both non-phosphor. and p56lck -phosphor. tau
-
Tau TAUY7
Biosource Invitrogen
polyclonal
Rabbit
(liquid, purified Ig, PBS, azide)
-
Human
immunogen = synthetic peptide, corresp. to aa residues 384-397 of human tau protein isoform 6 (AKTDHGAEIV-pY-KSP), conj. to KLH via an N-terminal cysteine residue
detects both non-phosphor. and p56lck -phosphor. tau
-
Tau TAUY8
Biosource Invitrogen
polyclonal
Rabbit
(liquid, purified Ig, PBS, azide)
-
Human
immunogen = synthetic peptide, corresp. to aa residues 384-397 of human tau protein isoform 8 (A K T D H G A E I V -pY- K S P), conjugated to KLH
detects both non-phosphor. and p56lck -phosphor. tau
-