human nucleii (235-1)
Chemicon and Upstate are Millipore
monoclonal
IgG1
Mouse
(liquid, supernatant, PBS, no presrvative, protein A purified)
-
Primate (various), Human
-
Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells
-
Mitochondria, surface (113-1)
Chemicon and Upstate are Millipore
monoclonal
IgG1
Mouse
(liquid, supernatant, purified, concentrate, PBS, azide)
-
Human
epitope on surface of intact mitochondria
recognizes a 60kDa nonglycosylated protein component of mitochondria found in human cells
-
Mitochondria
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(liquid, serum, purified, azide)
-
Human, Monkey
immunogen = Presenilin 1 peptide conjugated to KLH
specific to mitochondria; recognizes a 62 kD protein of immunoblots of subcellular fractions enriched in mitochondrial fractions
-
OxPhos Complex I, 39 kDa subunit (20C11)
Molecular Probes Invitrogen
monoclonal
IgG1
Mouse
(lyophilized)
-
Bovine, Human, Mouse, Rat
immunogen = OxPhos Complex I, isolated from bovine heart,bovine liver, and human heart
recognizes Complex I (NADH-ubiquinoloxidoreductase), 39 kDa subunit.
-
Lamp-1 (1D4B)
BioLegend
monoclonal
IgG2b , κ
Rat
(liquid, affinity purified, PBS, azide)
-
Mouse
immunogen = mouse embryo 3T3 cells
LAMP-1
-
Lamp-1 (1D4B)
BioLegend
monoclonal
IgG2b , κ
Rat
(liquid, affinity purified, PBS, azide, conj. FITC)
-
Mouse
immunogen = mouse embryo 3T3 cells
LAMP-1
-
Lamp-1 (1D4B)
BioLegend
monoclonal
IgG2b , κ
Rat
(liquid, affinity purified, PBS, azide, conj. Biotin)
-
Mouse
immunogen = mouse embryo 3T3 cells
LAMP-1
-
Lamp-1 (1D4B)
BioLegend
monoclonal
IgG2b , κ
Rat
(liquid, affinity purified, PBS, azide, conj. Alexa Fluor® 488)
-
Mouse
immunogen = mouse embryo 3T3 cells
LAMP-1
-
Lamp-1 (1D4B)
BioLegend
monoclonal
IgG2b , κ
Rat
(liquid, affinity purified, PBS, azide, conj. Alexa Fluor® 647)
-
Mouse
immunogen = mouse embryo 3T3 cells
LAMP-1
-
Mac-3 /LAMP-2 (M3/84)
BioLegend
monoclonal
IgG1 , κ
Rat
(liquid, PBS, azide, affinity purified)
-
Mouse
immunogen = membrane glycoproteins from C57BL/6 mouse peritoneal exudate cells
recognizes Mac-3 in macrophages, some myeloid cell lines
-
Lamp-1
DSHB U. Iowa
monoclonal
IgG2a
Rat
(liquid, affinity purified, PBS, azide)
-
Mouse
immunogen = Lamp-1 from NIH/3T3 mouse embryo fibroblast tissue culture cell membranes (epitope in N-terminus)
110 kDa Lamp-1; labels lysosome membranes
-
LAMP1
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, antisera, Tris, glycine, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide: CKRSHAGYQT , corresp. to amino acids 407-417 of Human LAMP1
detects LAMP1
-
LAMP2 -Lysosomal Marker (GL2A7)
Novus Biologicals
monoclonal
IgG
Rat
(liquid, ascites, PBS, glycerol, azide, protein-G purified)
-
Human, Mouse, Rat
immunogen = purified preparation of mouse liver lysosomal membranes
detects LAMP2; will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin
-
GM130 (35/GM130)
BD Pharmingen
monoclonal
IgG1 ,κ
Mouse
(aquaeous buffered solution, BSA, stabilizer, azide, affinity purified, Alexa Fluor® 488)
-
Dog, Human, Mouse, Rat
immunogen = rat C-terminal GM130 recombinant protein
recognizes GM130, regardless of phosphoryl. status
-
GM130 (NN 2C10/1)
Abcam
monoclonal
IgG2a
Mouse
(liquid, PBS, protein A purified)
-
Rat
immunogen = recombinant full length rat protein
recognizes GM130
-
GM130
Abcam
polyclonal
IgG
Rabbit
(liquid, BSA, azide, glycerol, Tris-glycine, citric acid, EDTA, NaCl)
-
Human
immunogen = synthetic peptide (human)
GM130
-
GM130
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
-
Drosophila
immunogen = synthetic peptide derived from within residues 750 to the C-terminus of fruit fly (Drosophila melanogaster) GM130, conjugated to KLH
GM130
-
GM130 (EP892Y)
Abcam
monoclonal
IgG
Rabbit
(liquid, PBS, azide, glycerol, Tris-glycine saline, EDTA, citric acid)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to residues near the N-term of human GM130
GM130
-
GM130
Abcam
polyclonal
IgG
Rabbit
(liquid, BSA, PBS, azide, BBS, EDTA, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide derived from within residues 950 to the C-terminus of rat GM130
GM130
-
GM130 (EP818Y)
Abcam
monoclonal
IgG
Rabbit
(liquid, supernatant, BSA, azide, glycerol, Tris-glycine saline)
-
Human
immunogen = synthetic peptide corresp. to residues near the N-term of human GM130
GM130
-
GM130
Abcam
polyclonal
IgG
Rabbit
(liquid, BSA, PBS, azide, affinity purified)
-
Drosophila
immunogen = synthetic peptide derived from within residues 1-100 of fruit fly (Drosophila melanogaster) GM130, conj. to KLH
GM130
-
GM130 (C-19)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
-
Human, Mouse, Rat
immunogen = epitope mapping at the C-terminus of human GM130
GM130
-
GM130 (H-7)
Santa Cruz
monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = raised against aa191-255 mapping within an internal region of human GM130
GM130
-
GM130 (B-10)
Santa Cruz
monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = raised against aa191-255 mapping within an internal region
GM130
-
GM130 (H-65)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin, affinity purified)
-
Human, Mouse, Rat
immunogen = epitope corresp. to aa191-255 mapping within an internal region of human GM130
GM130
-
GM130 (NN 2C10/1)
Santa Cruz
monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
-
Rat
immunogen = recombinant rat GM130
GM130
-
GM130 (4i375)
Santa Cruz
monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
-
Rat
immunogen = recombinant rat GM130
GM130
-
GM130 (P-15)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
-
Mouse, Rat
immunogen = epitope mapping within an internal region of rat GM130
GM130
-
GM130 (P-20)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
-
Human
immunogen = epitope mapping within an internal region of human GM130
GM130
-
GM130 (S-18)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
-
Mouse, Rat
immunogen = epitope mapping near the N-terminus of rat GM130
GM130
-
GM130 phos Ser25
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
-
Human, Mouse, Rat
immunogen = epitope corresp. to a short aa seq. containing phosphorylated Ser of human GM130
GM130 phosphorylated at Ser25
-
GM130 (EP892Y)
Novus Biologicals
monoclonal
IgG
Rabbit
(liquid, ascites, azide, glycerol, Tris-glycine saline, EDTA, citric acid)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to residues near the N-term of human GM130
GM130
-
GM130 (NN2C10/1)
Novus Biologicals
monoclonal
IgG2a
Mouse
(liquid, PBS, purified)
-
Human, Mouse, Rat
immunogen = recombinant full length protein (rat)
GM130
-
LAMP-1
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human, Mouse, Rat
immunogen = a 15 amino acid peptide from near the center of human LAMP-1
detects LAMP-1
-
LAMP-2
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human, Mouse
immunogen = a 17 amino acid peptide from near the center of human LAMP-2
detects LAMP-2
-
GM130
Cell Signaling
polyclonal
Rabbit
(liquid, HEPES, saline, BSA, glycerol)
-
Human, Monkey
immunogen = a synthetic peptide corresponding to the amino-terminal sequence of human GM130
detects endogenous levels of total GM130 protein
-
LAMP-1 (C54H11)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Human, Monkey, Mouse
immunogen = a synthetic peptide surrounding Ser140 of human LAMP1
detects endogenous levels of total LAMP1 protein
-
Lamp-2 (H4B4/CD107b)
DSHB U. Iowa
monoclonal
IgG1
Mouse
(liquid, available as supernatant, concentrate, ascites)
-
Human
immunogen = human Lamp-2
Lamp-2
-
GM130
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, BSA, azide)
-
Human
immunogen = synthetic peptide corresp. to residues G(a.a. 368)QVMESVRQ LQMERDK(a.a. 383) of human GM130
detects GM130; an approx. 135 kDa band
-
GM130
Novus Biologicals
polyclonal
IgG
Mouse
(liquid, antisera, no preservativeAlso available purified, H00002801-B01P)
-
Human
immunogen = GOLGA2 (1 a.a. ~ 345 a.a) full-length human protein
detects GOLGA2/GM130; reactive against immunizing recombinant protein, transfected lysate and tissue lysate for WB
-
Giantin (6D4)
Novus Biologicals
monoclonal
IgG1 ,κ
Mouse
(liquid, purified IgG, PBS, no preservative)
-
Human
immunogen = GOLGB1 (NP_004478, 3 a.a. ~ 92 a.a) partial recomb. protein with GST tag
detects giantin; reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control
-
Giantin (G1/133)
Alexis Enzo Life Sciences
monoclonal
IgG1
Mouse
(affinity purified, gel filtrated (FPLC), lyophilized)
-
Mammal (various), Avian (various), Human, Monkey
immunogen = Giantin from Caco-2 cell Golgi membrane fraction
Recognizes giantin
-
Lamp-2 /lgp96
Zymed/ Invitrogen
polyclonal
Ig
Rabbit
(serum, affinity purified, PBS, azide)
-
Rat
immunogen = synthetic peptide derived from the cytosolic region of the rat Lamp-2 (lgp96) protein
detects the ~96 kDa rat Lamp-2 (lgp96)
-
Lamp-2 /CD107b (ABL-93)
BD Pharmingen
monoclonal
IgG2a , κ
Rat
(liquid, affinity purified, aqueous, buffered, azide, FITC conj.)
-
Mouse
immunogen = glycoproteins purified from BALB/c mouse embryo 3T3 cell line
detects LAMP-2
-
GM130 (35/GM130)
BD Transduction
monoclonal
IgG1 ,κ
Mouse
(aquaeous buffered solution, BSA, protein stabilizer, azide, Alexa Fluor® 555 conj.)
-
Dog, Human, Mouse, Rat
immunogen = Rat GM130, aa. 869-982
recognizes GM130, 130 kDa
-
GM130 (35/GM130)
BD Transduction
monoclonal
IgG1 ,κ
Mouse
(aquaeous buffered solution, BSA, protein stabilizer, azide, Alexa Fluor® 488 conj.)
-
Dog, Human, Mouse, Rat
immunogen = Rat GM130, aa. 869-982
recognizes GM130, 130 kDa
-
GM130 (35/GM130)
BD Transduction
monoclonal
IgG1 ,κ
Mouse
(aquaeous buffered solution, BSA, protein stabilizer, azide, Alexa Fluor® 647 conj.)
-
Dog, Human, Mouse, Rat
immunogen = Rat GM130, aa. 869-982
recognizes GM130, 130 kDa
-
Lamp-2
DSHB U. Iowa
monoclonal
IgG2a ,κ
Rat
(liquid, affinity purified, PBS, azide)
-
Mouse
immunogen = Lysosomal membrane glycoprotein B (lgp-B), from mouse liver homogenate
110 kDa Lamp-2; labels presumptive lysosomes and late endosomes
-
LAMP1, N-terminal
ABGENT
polyclonal
Ig
Rabbit
(liquid, PBS, azide, Protein A affinity purified)
-
Human, Mouse
Immunogen = KLH conjugated synthetic peptide between 131~161 amino acids from the N-terminal region of human LAMP1
recognizes LAMP1, 44.8 kDa by WB
-
LAMP1 (H4A3)
Abcam
monoclonal
IgG1
Mouse
(liquid, IgG fraction, borate, no preservativeAlso available as Phycoerythrine conj. ab25367)
-
Human, Monkey, Mouse
-
recognizes LAMP1, ~48 kDa by WB
-
Tom20 (FL-145)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = peptide corresp. to amino acids 1-145 representing full length Tom20 of human origin
recognizes Tom20
-
Opa1 (V-20)
Santa Cruz
polyclonal
IgG
Goat
(liquid, affinity purified, PBS, azide, gelatin)
-
Human, Mouse
immunogen = apeptide mapping within an internal region of OPA1 (dynamin family) of human origin.
for detection of Optic atrophy 1 gene protein
-
Mitofilin
Chemicon and Upstate are Millipore
polyclonal
IgG1
Rabbit
(liquid, raw rabbit serum)
-
Human, Mouse, Rat
-
recognizes mitofilin, mitochondrion inner membrane protein
-
GM130 (35/GM130)
BD Transduction
monoclonal
IgG1 ,κ
Mouse
(aquaeous buffered solution, BSA, glycerol, azide, FITC conj.)
-
Dog, Human, Mouse, Rat
immunogen = Rat GM130, aa. 869-982
recognizes GM130, 130 kDa
-
Omi/HtrA2 (229926)
R&D Systems
monoclonal
IgG2a
Mouse
(filtered solution, protein-G affinity purified, PBS, trehalose, lyophilized)
-
Human
immunogen = purified, E. coli-derived, recombinant human High Temperature Requirement Protein-2 (rhHtrA2; aa 134-458)
detects the mitochondria-processed form of endogenous human HtrA2/Omi.
-
Tim23 (32/Tim23)
BD Transduction
monoclonal
IgG2a
Mouse
(Aqueous buffered solution, affinity purified, BSA, glycrol, azide)
-
Human, Mouse, Rat
immunogen = rat Tim23 aa. 5-126.
detects Tim23, 23 kDa by WB
-
Lamp-2
Sigma Aldrich
polyclonal
Rabbit
(buffered aqueous solution, affinity isolated, PBS, azide)
-
Mouse, Rat
immunogen = a synthetic peptide corresponding to amino acid residues 404-415 of mouse LAMP2 with N-terminal added cysteine, conjugated to KLH
detects LAMP-2
-
Omi/HtrA2
R&D Systems
polyclonal
IgG
Rabbit
(filtered solution, affinity purified, PBS, trehalose, lyophilized)
-
Human, Mouse
immunogen = E. coliderived recombinant human HTRA2/Omi Ala134Glu45
detects full length and mitochondria processed HTRA2/Omi
-
GM130 (35/GM130)
BD Transduction
monoclonal
IgG1 ,κ
Mouse
(aquaeous buffered solution, BSA, glycerol, azide, affinity purified)
-
Dog, Human, Mouse, Rat
immunogen = Rat GM130, aa. 869-982
recognizes GM130, 130 kDa
-
Lamp-2 /CD107b (H4B4)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin.Also available as sc-18822 PE or sc-18822 FITC conjugates for FC, and Alexa Fluor® for FC or IF)
-
Human
immunogen = adherentspleen cells of human origin
detects LAMP-2
-