VGLUT1/2
Synaptic Systems
polyclonal
Rabbit
(lyophilized. affinity purified with RSA for stabilization)
-
Mammal (various), Chicken, Zebra fish
immunogen = Synthetic peptide SQPAYFEE VFGFEISK (aa 324-339 in rat VGLUT 1 and aa 332-347 in rat VGLUT 2) forced into a loop-shaped structure
epitope identical in VGLUT 1 and VGLUT 2, one mismatch in VGLUT 3
-
NMDA-Receptor 1
Synaptic Systems
polyclonal
Rabbit
(affinity purified Ig, PBS, RSA for stabilization, lyophilized)
-
Human, Mouse, Rat
Immunogen = synthetic peptide (aa 35-53 of rat NMDA R1)coupled to KLH
specific for NMDA-receptor 1
-
NMDA-Receptor 1
Synaptic Systems
polyclonal
Rabbit
(affinity purified Ig, PBS, RSA for stabilization, lyophilized)
-
Human, Mouse, Rat
Immunogen = synthetic peptide (aa 35-53 of rat NMDA R1)coupled to KLH
specific for NMDA-receptor 1
-
NMDA-Receptor 1 (M68)
Synaptic Systems
monoclonal
IgG2b
(ascites, PBS, lyophilized)
-
Human, Mouse, Rat
Immunogen = recomb. protein containing aa 660-811 of the NMDA R1 receptor
-
-
NMDA-Receptor 1 (M68)
Synaptic Systems
monoclonal
IgG2b
(affinity purified IgG, lyophilized)
-
Human, Mouse, Rat
Immunogen = recomb. protein containing aa 660-811 of the NMDA R1 receptor
-
-
VGLUT1 /BNPI
Synaptic Systems
polyclonal
Rabbit
(lyophilized.antiserum or affinity purified with RSA for stabilization)
-
Cow, Human, Mouse, Rat
immunogen = Strep-Tag® fusion protein of rat VGLUT 1 (aa 456 - 560)
specific to VGLUT1; highly recommended as markers for glutamatergic nerve terminals and give excellent results in ICC
-
NMDAε1 (C-17)
Santa Cruz
polyclonal
IgG
Goat
(liquid, affinity purified, PBS, gelatin, azide)
-
Human, Mouse, Rat
Immunogen = peptide mapping at the C-terminus of human NMDAε1
detects NMDAε1
-
NMDAε1 (D-18)
Santa Cruz
polyclonal
IgG
Goat
(liquid, affinity purified, PBS, gelatin, azide)
-
Human, Mouse, Rat
Immunogen = peptide mapping within an extracellular domain of NMDAε1 ofhuman origin
detects NMDAε1
-
NMDAε1 (H-54)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
Immunogen = aa 23-76 within an extracellular domain of human NMDAε1
detects NMDAε1
-
NMDAε1 (K-18)
Santa Cruz
polyclonal
IgG
Goat
(liquid, affinity purified, PBS, gelatin, azide)
-
Human, Mouse, Rat
Immunogen = peptide mapping within an internal region of NMDAε1 ofhuman origin
detects NMDAε1
-
NMDAε1 (N-20)
Santa Cruz
polyclonal
IgG
Goat
(liquid, affinity purified, PBS, gelatin, azide)
-
Human, Mouse, Rat
Immunogen = peptide mapping within an N-terminal extracellular domain of NMDAε1 ofhuman origin
detects NMDAε1
-
GluR-1 phos Ser831
Autogen Bioclear
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
Immunogen = short aa sequence containing phosphorylated Ser831 of human GluR-1
Ser831 phosphorylated GluR-1
-
GluR-1 phos Ser831
Autogen Bioclear
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
Immunogen = short aa sequence containing phosphorylated Ser831 of human GluR-1
Ser831 phosphorylated GluR-1
-
GluR-1 phos Ser845
Autogen Bioclear
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
Immunogen = short aa sequence containing phosphorylated Ser 845 of human GluR-1
Ser845 phosphorylated GluR-1
-
GluR-1 phos Ser845
Autogen Bioclear
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
Immunogen = short aa sequence containing phosphorylated Ser 845 of human GluR-1
Ser845 phosphorylated GluR-1
-
GluR-1 phos Ser863
Autogen Bioclear
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = short aa sequence containing phosphorylated Ser 863 of human GluR-1
Ser 863 phosphorylated GluR-1
-
GluR-1 phos Ser863
Autogen Bioclear
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = short aa sequence containing phosphorylated Ser 863 of human GluR-1
Ser 863 phosphorylated GluR-1
-
GluR-2
Autogen Bioclear
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = peptide mapping at the C-terminus of human GluR-2
GluR-2 and to a lesser extent GluR-3
-
GluR-2/3 phos Ser880/891
Autogen Bioclear
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
Immunogen = short aa sequence containing phosphorylated Ser880 and Ser891 of human GluR-2/3
Ser880 and Ser891 dually phosphorylated GluR-2 and GluR-3
-
NMDAε2
Autogen Bioclear
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
Immunogen = aa 27-76 mapping within an extracellular domain of human NMDAε 2
glutamate (NMDA) receptor subtype ε 2
-
NMDAε3
Autogen Bioclear
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = peptide mapping at the C-terminus of mouse NMDAε 3
glutamate (NMDA) receptor ε 3 subtype and to a lesser extent ε 4 subtype
-
NMDAε4
Autogen Bioclear
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = peptide mapping at the C-terminus of mouse NMDAε 4
glutamate (NMDA) receptor ε 4 subtype and to a lesser extent ε 3 subtype
-
GluR-2/3 phos Ser880/891
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
Immunogen = short aa sequence containing phosphorylated Ser880 and Ser891 of human GluR-2/3
reacts with Ser880 and Ser891 dually phosphorylated GluR-2 and GluR-3
-
GluR1
Upstate and Chemicon are Millipore
polyclonal
IgG
Rabbit
(liquid, protein-A purified, Tris-Glycine, saline, glycerol, azide)
-
Rat
Immunogen = KLH-conjugated, synthetic peptide (C-RDHTR VDWKRPK) corresp. to aa. 276-287 of rat glutamate receptor 1 (GluR1), with a cysteine added for conjugation
detects GluR1; ~106 kDa
-
GluR1 phos Ser845
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(liquid, Hepes, NaCl, BSA, glycerol, affinity purified)
-
Rat
Immunogen = synthetic peptide corresponding to aa surrounding the phosphoSer845 of rat GluR1
GluR1 phosphorylated Ser845 (100kDa)
-
GluR1 (EPR2148)
Upstate and Chemicon are Millipore
monoclonal
IgG
Rabbit
(liquid, unpurified, Tris-Glycine, saline, glycerol, azide, BSA)
-
Human, Mouse, Rat
Immunogen = phospho-specific peptide corresponding to residues surrounding Ser845 of human GluR1
recognizes GluR1 phosphorylated on Ser845; ~106 kDa
-
NMDAR2B (13)
BD Transduction
monoclonal
IgG2b
Mouse
(liquid, BSA, glycerol, azide)
-
Human, Mouse, Rat
Immunogen = aa 892-1051 of rat NMDAR2B
detects NMDAR2B
-
NMDAR1 (54.1)
BD Transduction
monoclonal
IgG2a
Mouse
(liquid, purified, aqueous buffered solution, azide)
-
Human, Monkey, Rat
Immunogen = rat NMDAR1 aa 660-811, near the C-terminus
detects NMDAR1; no cross with NMDAR2-5 receptors
-
NMDAR2A (5)
BD Transduction
monoclonal
IgG1
Mouse
(liquid, affinity purified, BSA, glycerol, azide)
-
Mouse, Rat
Immunogen = mouse NMDAR2A aa 1093-1214 at C-terminus
detects NMDAR2A
-
NR1 phos Ser896
Upstate and Chemicon are Millipore
polyclonal
IgG
Rabbit
(liquid, tris-glycine saline, BSA, azide, glycerol, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic peptide (K F K R R R pS S K D T S T) corresp. to aa 891-902 of rat NR1A coupled to thyroglobulin
detects NR1 phosphorylated on serine 896
-
NR1 phos Ser896/897 and pan
Upstate and Chemicon are Millipore
kit
IgG
Rabbit
(sampler kit: NR1 Ser896; NR1 Ser897; NR1 pan)
-
Human, Mouse, Rat
-
detects NR1
-
NR1 phos Ser897
Upstate and Chemicon are Millipore
polyclonal
IgG
Rabbit
(liquid, tris-glycine saline, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic peptide (K F K R R R S pS K D T S T) corresp. to aa 891-902 of rat NR1A, thyroglobulin conjugated
detects NR1 phosphorylated on serine 897 and NR1 dually phosphorylated on serine 896 and 897
-
NR1, C-term
Upstate and Chemicon are Millipore
polyclonal
IgG
Rabbit
(liquid, tris-glycine saline, azide, affinity purified)
-
Rabbit, Human, Monkey, Mouse, Rat
Immunogen = synthetic peptide (K R R A I E R E E G Q L Q L C S R H R E S) corresp. to the C-terminal of rat NR1 found in splice variant isoforms A, B, C and F
detects NR1 (120 kDa)
-
NR1, C-term splice var.
Upstate and Chemicon are Millipore
polyclonal
IgG
Rabbit
(liquid, tris-glycine saline, azide, Protein A purified)
-
Monkey, Mouse, Rat
Immunogen = synthetic peptide (K Q Y H P T D I T G P L N L S D P S V S T V V) corresp. to the C-terminus of rat NR1 splice variant isoform D
detects NR1 splice variants (120 kDa)
-
NR1, pan C-term
Upstate and Chemicon are Millipore
monoclonal
IgG
Mouse
(liquid, tris-glycine saline, glycerol, azide, Protein G purified)
-
Human, Mouse, Rat
Immunogen = His tag fusion protein corresp. to aa 834-938 of rat NR1A; the epitope maps to a region (834-864) common to all NR1 isoforms
detects NR1; should cross react with all NR1 isoforms (130 kDa)
-
GluR1
Anaspec
polyclonal
Rabbit
(liquid, affinity purified, PBS, BSA, azide)
-
Human, Mouse, Rat
immunogen = a synthetic peptide from the C-terminus of human GluR1 protein
reacts with GluR1
-
mGluR1
Anaspec
polyclonal
Rabbit
(liquid, affinity purified, PBS, BSA, azide)
-
Chicken, Human, Monkey, Mouse, Rat
immunogen = a synthetic peptide from the C-terminus of human mGluR1 protein
reacts with mGluR1
-
AMPA Receptor (GluR 2/3/4)
Cell Signaling
polyclonal
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, Protein A affinity purified)
-
Human, Mouse, Rat
Immunogen = a synthetic peptide corresp. to residues surrounding Ser720 of human GluR2
detects endogenous levels of total GluR 2/3/4 protein; may also detect GluR1
-
NMDAR1
Cell Signaling
polyclonal
Rabbit
(liquid, Hepes, saline, BSA, glycerol, Protein A purified)
-
Human, Mouse, Rat
Immunogen = a synthetic peptide corresponding to human NMDAR1
detects endogenous levels of total NMDAR1 protein
-
NMDAR2A
Cell Signaling
polyclonal
Rabbit
(liquid, Hepes, saline, BSA, glycerol, affinity purified)
-
Mouse, Rat
Immunogen = a synthetic peptide corresponding to human NMDAR2A
detects endogenous levels of total NMDAR2A protein
-
NMDAR2B
Cell Signaling
polyclonal
Rabbit
(liquid, HEPES, saline, BSA, glycerol, affinity purified)
-
Human, Mouse, Rat
Immunogen = a synthetic peptide corresponding to human NMDAR2B
detects endogenous levels of total NMDAR2B protein, 190 kDa by WB
-
NMDAR1 phos Ser896
Cell Signaling
polyclonal
Rabbit
(liquid, Hepes, NaCl, BSA, glycerol, Protein A purified)
-
Human, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to the residues around Ser896 of human NMDAR1
Detects overexpressed NMDAR1 only when phosphorylated at serine 896
-
NMDAR1 phos Ser897
Cell Signaling
polyclonal
Rabbit
(liquid, Hepes, NaCl, BSA, glycerol, Protein A purified)
-
Human, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues around Ser897 of human NMDAR1
Detects overexpressed NMDAR1 only when phosphorylated at serine 897
-
NMDAR1 phos Ser890
Cell Signaling
polyclonal
Rabbit
(liquid, Hepes, NaCl, BSA, glycerol, Protein A purified)
-
Human, Mouse, Rat
Immunogen = synthetic phospho-peptide corresponding to residues around serine 890 of human NMDAR1
Detects overexpressed and endogenous NMDAR1 only when phosphorylated at Ser890
-
GluR2 (1B5)
Novocastra
monoclonal
IgG2b
Mouse
(lyophilized supernatant, azide)
-
Human
Immunogen = recombinant prokaryotic fusion protein corresp. to a region of the extracellular N-terminal region of human GluR2
GluR2
-
GluR 1 /GluA 1
Synaptic Systems
polyclonal
IgG2b
Rabbit
(affinity purified IgG, PBS, rabbit serum albumin was added for stabilization, lyophilizedAlso available as monoclonal mouse, 182011)
-
Human, Mouse, Rat
Immunogen = recombinant protein of mouse GluA 1 (C-terminus; aa 828 - 906).
specific for GluA 1
-
GluR 1 /GluA 1 (160E5)
Synaptic Systems
monoclonal
IgG2b
Mouse
(affinity purified IgG, PBS, azide, lyophilizedAlso available as rabbit, affinity purified, 182003)
-
Human, Mouse, Rat
Immunogen = synthetic peptide (aa 895 - 907 in rat GluA 1) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue
specific for GluA 1
-
NMDAR2B, C-term
Sigma Aldrich
polyclonal
Rabbit
(lyophilized, NH4HCO3, affinity purified)
-
Human, Mouse, Rat
Immunogen = amino acids 984-1104 at C-terminus of NR2B
NMDAR2B (180kDa). No cross-reaction with NR2A aor NR2C subunits
-
NMDA R2B phos Ser1480
Pierce Thermo Scientific
polyclonal
IgG
Rabbit
(liquid, affinity purified, HEPES, saline, BSA, glycerol)
-
Rat
immunogen = synthetic phosphopeptide corresponding to a portion of rat NMDA NR2B Subunit around serine 1480
detects Phospho-NMDA NR2B Subunit (Ser1480)
-
NR2A
Upstate and Chemicon are Millipore
polyclonal
IgG
Rabbit
(liquid, purified, buffer containing storage buffer, Tris-glycine, NaCl, sodium azide and glycerol)
-
Mouse, Rat
Immunogen = 6His-tagged fusion protein corresponding to amino acids 1265-1464 of mouse NMDA receptor 2A (NR2A)
recognizes NR2A, Mw ~170 kDa
-
GluR1 (C3T)
Upstate and Chemicon are Millipore
monoclonal
IgG
Rabbit
(cultured supernantant in sodium azide)
-
Chimpanzee, Human, Mouse, Rat
Immunogen = peptide (TLPRNS GAGAS) corresp. to aa. 858-868 of mature human GluR1
recognizes GluR1, 106 kDa
-
GluR1 (RH95)
Chemicon and Upstate are Millipore
monoclonal
IgG2a ,κ
Mouse
(liquid, purified, buffer with Tris-glycine, saline, sodium azide)
-
Rat
Immunogen = Thyroglobulin conjugated linear peptide of rat GluR1 designed around the N-terminus
recognizes GluR1 at the N-terminus
-
GluR1
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(lyophilized from PBS, BSA, azide, affinity purified)
-
Equine, Human, Mouse, Rat
Immunogen = KLH-conjugated linear peptide corresponding to human Glutamate receptor 1 at the cytoplasmic domain
detects GluR1, cytoplasmic domain
-
NMDA NR1
Pierce Thermo Scientific
monoclonal
Mouse
(lyophilized, affinity purified)
-
Rat
Immunogen = fusion protein corresponding to amino acids 1-564 of rat NMDA NR1
detects NMDA NR1, ~100 kDa in WB; shows no cross-reactivity with any of the NR2 subunits
-
NMDA NR1
Pierce Thermo Scientific
polyclonal
Rabbit
(lyophilized, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic peptide corresponding to residues (849)R A E P D P K K K A T F R A(862) C of rat NR1 exon 21
detects NMDA NR1, ~120 kDa in WB
-
NMDA NR1
Pierce Thermo Scientific
polyclonal
Rabbit
(lyophilized, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic peptide corresponding to residues (195)N Y E N L D Q L S Y D N K R G P(210) C of Exon 5 of rat NMDA Receptor Type 1
detects NMDA NR1, ~120 kDa in WB
-
NMDAR2B phosphoTyr1472
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(liquid, Hepes, NaCl, BSA, glycerol, azide, affinity purified Ig)
-
Rat
Immunogen = synthetic peptide corresp. to aa surrounding phosphoTyr1472 of rat NMDAR 2B
NMDAR 2B phosphorylated Tyr1472 (~180 kDa)
-
GluR-1
Calbiochem EMD Biosciences
polyclonal
IgG
Rabbit
(liquid, phosphate buffer, gelatin, azide)
-
Rat
Immunogen = synthetic peptide (RTSDSRDH TRVDWKR) corresp. to aa271-285 of rat GluR1 (numbered from the signal peptide)
recognizes the ~105-107 kDa GluR1 protein. Also cross-reacts with a 50 kDa protein in rat brain extracts
-
GluR2 (6C4)
BD Pharmingen
monoclonal
IgG2a
Mouse
(liquid, buffer, azide)
-
Dog, Monkey, Rat
Immunogen = recombinant trpE fusion protein containing the putative N-terminal aa 175-430 of GluR2
GluR2
-
NMDAR NR2B phos Tyr1472
Phospho- solutions
polyclonal
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified)
-
Human, Mouse, Rat
Immunogen = phosphopeptide corresp. to aa residues surrounding the anti-phospho-Try1472 of NMDA Receptor NR2B
~180 NMDA Receptor NR2B-subunit phosphorylated at Tyr1472, also labels ~65k and ~115k.
-
NR2B
Chemicon and Upstate are Millipore
polyclonal
IgG
Rabbit
(liquid, tris-glycine saline, azide, Protein G purified)
-
Bovine, Dog, Human, Monkey, Mouse, Rat
Immunogen = peptide comprising the C-terminal 20 aa of mouse NR2B (K F N G S S N G H V Y E K L S S I E S D V)
NR2B
-
NR1 /NMDAR1 C-term
Chemicon and Upstate are Millipore
monoclonal
IgG
Mouse
(liquid, purified IgG, Tris-glycine, saline, azide)
-
Human, Mouse, Rat
Immunogen = His-tag fusion protein corresponding to residues 834-938 of rat NR1A; epitope maps to a region (a.a. 834-864) common to all NR1 isoforms
detects NMDAR1, 130 kDa by WB
-
NR2A
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(liquid, unpurified serum, azide)
-
Mouse, Rat
immunogen = GST-tagged recombinant protein corresponding to rat NR2A at the C-terminus
recognizes NR2A (~176 kDa) at the C-terminus
-
NR2A
Chemicon and Upstate are Millipore
polyclonal
IgG
Rabbit
(liquid, tris-glycine saline, glycerol, azide, Protein A purified)
-
Mouse, Rat
immunogen = 6His-tagged fusion protein corresp.to aa1265-1464 of mouse NMDA receptor 2A (NR2A).
NR2A (170 kDa)
-