|
|
US Biological |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purifiedavailable without azide, cat# 18700-15A) |
IP, WB |
Human, Mouse, Rat |
Immunogen = GST fusion protein containing aa976-1094 of mouse IRS2 |
Recognizes mouse IRS-2 (170-185kD) |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid antisera, Tris-citrate or Tris-phosphate, azide) |
IP |
Human |
(epitope maps to a region between residue 750 and 800 of human insulin receptor) |
directed against mature 17kDa human IL-1beta |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid,, PBS, glycerol, azide, affinity purified) |
ELISA, IC, IF, IH, IP, WB |
Human, Mouse, Rat |
Immunogen = synthetic non-phosphopeptide derived from human IRS1 around the phosphorylation site of serine 307 (T-E-S-I-T) |
detects endogenous levels of total IRS1 protein |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
Immunogen = synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of serine 636 or serine 639 (M-Sp -P-K -Sp) |
detects endogenous levels of IRS1 only when phosphorylated at Serine 636 or 639 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, TBS, no preservative, affinity purified) |
ELISA, IH, IP, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide corresponding to the epitope, D G Y M P, with a single phosphorylation site, Tyr612, from human IRS1 |
IRS1 when phosphorylated at Tyr 612 |
- |
|
|
Abcam |
monoclonal
IgG |
Rabbit
(liquid, tissue culture supernatant, Tris glycine saline, azide, glycerol, BSA) |
IC, IF, IH, IP, WB |
Human |
Immunogen = synthetic peptide corresp. to residues near the C-terminus of human IRS-1 |
IRS1 (170kDa) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
Immunogen = synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of serine 1101 (H-S-Sp-E-T) |
detects endogenous levels of IRS1 only when phosphorylated at serine 1101 |
- |
|
|
Abcam |
monoclonal
IgG |
Rabbit
(liquid, tissue culture supernatant, Tris glycine saline, azide, glycerol, BSA) |
IC, IF, IH, IP, WB |
Human |
Immunogen = synthetic peptide corresp. to residues near the N-terminus of human IRS-2 |
IRS2 (170kDa) |
- |
|
|
Abcam |
monoclonal
IgG |
Rabbit
(liquid, tissue culture supernatant, Tris glycine saline, azide, glycerol, BSA) |
FC, IH, IP, WB |
Human |
Immunogen = synthetic peptide corresp. to residues near the C-terminus of human IRS-4 |
IRS4 (Insulin receptor substrate 4) (133kDa) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, TBS, no preservative, affinity purified) |
ELISA, IH, IP, WB |
Human, Mouse, Rat |
Immunogen = synthetic phosphopeptide corresp. to the epitope A T Sp A, with a single phosphorylation site, Ser 312, from human IRS1 |
detects endogenous levels of IRS1 only when phosphorylated at serine 312 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide derived from a region of IRS 1 that contains serine 616 |
detects levels of IRS1 only when phosphorylated at serine 616 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
ELISA, IP, WB |
Human, Mouse |
Immunogen = synthetic peptide (Y K A Sp S P A E S S), corresp. to aa727-736 of human IRS2 at the phosphorylated site of Serine 731 |
IRS2 phosphorylated at ser731; not other phosphorylated sites or non-phosphorylated IRS 1 or IRS 2 |
- |
|
|
Abcam |
monoclonal
IgG |
Rabbit
(liquid, tissue culture supernatant, Tris glycine saline, azide, glycerol, BSA) |
IH, IP, WB |
Human |
Immunogen = synthetic peptide corresp. to residues surrounding Ser1203 of human IRS2 |
IRS2 (170kDa) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified) |
WB |
Chicken, Human, Mouse, Rat |
Immunogen = synthetic peptide derived from a region of IRS 1 that contains tyrosine 612 |
IRS1 when phosphorylated at Tyr 612; IRS 2 (85% homologous) has not been tested |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum, azide) |
IC, IF, IP, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide corresp. to the C-terminal 14 residues (Rat) conj. to KLH |
IRS1 protein |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, BSA, affinity purified) |
IC, IF, WB |
Human, Mouse |
Immunogen = synthetic peptide derived from within residues 1300 to the C-terminus of mouse IRS2, conj. to KLH |
IRS2 (170kDa) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, BSA, affinity purified) |
IC, IF, WB |
Human, Mouse |
Immunogen = synthetic peptide derived from within residues 1050-1150 of mouse IRS2, conj. to KLH |
IRS2 (170kDa) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified) |
ELISA, IH |
Human, Mouse, Rat |
Immunogen = synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 307 (T-E-Sp-I-T) |
detects levels of IRS1 only when phosphorylated at serine 307 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified) |
WB |
Pig, Human, Mouse, Rat |
Immunogen = synthetic peptide (human) derived from a region of IRS 1 that contains serine 312 |
detects levels of IRS1 only when phosphorylated at serine 307 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified) |
ELISA, IH |
Human, Mouse, Rat |
Immunogen = synthesized phosphopeptide derived from human IRS1 around the phosphoryation site of serine 323. |
detects endogenous levels of IRS1 when phosphorylated at serine 323 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified) |
ELISA, IH |
Human, Mouse, Rat |
Immunogen = synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of serine 612 (P-M-Sp-P-G). |
detects endogenous levels of IRS1 only when phosphorylated at serine 616 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified) |
ELISA, IH |
Human, Mouse, Rat |
Immunogen = synthesized phosphopeptide derived from human IRS1 around the phosphorylation site of serine 636 (P-M-Sp-P-K). |
detects IRS-1 only when phosphorylated at serine 636 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified) |
ELISA, IH |
Human, Mouse, Rat |
Immunogen = synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 639 (P-K-Sp-V-S). |
detects IRS-1 only when phosphorylated at serine 639; in rat and mouse the phosphorylation site is at serine 635 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified) |
ELISA, IH |
Human, Mouse, Rat |
Immunogen = synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 794 (S-T-Sp-S-G). |
detects IRS-1 only when phosphorylated at serine 794 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, glycerol, azide, affinity purified) |
WB |
Human |
Immunogen = synthetic peptide (human) derived from a region of IRS-1 that contains tyrosine 941 |
IRS1 when phosphorylated at Tyr 941 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
ELISA, IP, WB |
Human |
Immunogen = synthetic peptide: I T A T S P A S, corresp. to aa308-315 of human IRS-1;in mouse and rat, the synthetic peptide is a portion of aa303-310 |
IRS1 protein |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
ELISA, IH |
Human, Mouse, Rat |
Immunogen = synthesized non- phosphopeptide derived from human IRS1 around the phosphorylation site of serine 636 |
detects endogenous levels of total IRS1 protein |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified) |
ELISA, IH |
Human, Mouse, Rat |
immunogen = synthesized non-phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 312 (A-T-Sp-O-S) |
IRS-1 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
ELISA, IH |
Human, Mouse, Rat |
Immunogen = synthesized non- phosphopeptide derived from human IRS1 around the phosphorylation site of serine 639 (P-K-Sp-V-S) |
detects endogenous levels of total IRS1 protein |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
ELISA, IH |
Human, Mouse, Rat |
Immunogen = synthesized non- phosphopeptide derived from human IRS1 around the phosphorylation site of serine 312 (A-T-Sp-O-S) |
specific for IRS-1 protein |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
ELISA, IH |
Human, Mouse, Rat |
Immunogen = synthetic non- phosphopeptide derived from human IRS1 around the phosphorylation site of serine 307 (T-E-Sp-I-T) |
specific for IRS-1 protein |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
ELISA, IH |
Human, Mouse, Rat |
Immunogen = synthetic non- phosphopeptide derived from human IRS1 around the phosphorylation site of serine 636 or serine 639 (M-S-P-K-S) |
detects endogenous levels of total IRS1 protein |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
ELISA, IH |
Human, Mouse, Rat |
Immunogen = synthetic non-phosphopeptide derived from human IRS1 around the phosphorylation site of serine 323 |
IRS1 protein |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
ELISA, IH |
Human |
Immunogen = synthetic non-phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 794 (S-T-Sp-S-G) |
IRS1 protein |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, TBS, azide, BSA, affinity purified) |
IH |
Human |
Immunogen = synthetic peptide corresp. to a region between residues 1288-1338 of human IRS2 (NP_003740.2) |
IRS2 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, KPBS, BSA, azide, affinity purified) |
WB |
Pig, Human, Mouse, Rat |
Immunogen = synthetic phospho peptide: G L T R R S R T E Sp I T A T S P A S corresp. to aa298-316 of human IRS-1, conj. to KLH. |
detects IRS-1 only when phosphorylated at serine 307 (180kDa) |
- |
|
|
Abcam |
polyclonal
IgG |
Sheep
(liquid, HEPES, NaCl, azide, affinity purified) |
WB |
Chicken, Human, Mouse, Rat |
Immunogen = synthetic peptide: C F R K R T H Sp A G T corresp. to aa521-530 of human IRS1, conjugated to KLH. |
detects IRS-1 only when phosphorylated at serine 522 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, glycerol, azide, affinity purified) |
WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide derived from a region of mouse IRS-1 that contains tyrosine 1173 (corresp. to tyrosine 1179 of the human seq.) |
IRS1 when phosphorylated at Tyr 1179; IRS-3 (50% homologous) also may react, but has not been tested |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
WB |
Chicken, Human |
Immunogen = synthetic peptide derived from a region of human IRS-1 that contains tyrosine 1229 |
IRS1 when phosphorylated at Tyr 1229 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide derived from a region of IRS 1 that contains tyrosine 896 |
IRS1 when phosphorylated at Tyr 896 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, glycerol, thimerosal, affinity purified) |
IH, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide mapping to the C- terminus of human IRS1 |
recognizes IRS1; no reaction to IRS2, IRS3 and IRS4 |
- |
|
|
Abcam |
polyclonal
IgG |
Sheep
(liquid, HEPES, NaCl, azide, affinity purified) |
WB |
Chicken, Pig, Human, Mouse, Rat |
Immunogen = synthetic peptide: C S I S F Q K Q P E D R Q corresp. to C-terminal aa1231-1242 of human IRS1, conj. to KLH |
recognizes IRS1(200kDa) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, BSA, Tris, azide, affinity purified, prediluted) |
IH |
Chicken, Human, Mouse, Rat |
Immunogen = synthetic peptide: S E D L S A Y A S I S F Q K Q P E D R Q, corresp. to amino acids 1223-1242 of human IRS1 |
recognizes IRS1 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole serum, no preservative) |
IP, WB |
Human, Hamster, Mouse, Rat |
Immunogen = enzyme tagged protein, corresp. to C-terminal aa1122-1321 of mouse IRS2 |
IRS2 (170kDa); no cross with IRS-1 |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, no preservative, protein G purified) |
WB |
Human |
Immunogen = recombinant fragment, corresp. to aa850-951 of human IRS4 |
IRS4 (insulin receptor substrate 4) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, glycerol, azide) |
WB |
Human |
Immunogen = synthetic peptide (human) |
IRS4 (insulin receptor substrate 4) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
WB |
Mouse |
Immunogen = synthetic peptide: E S I T A T Sp P A S M V G G K, corresp. to aa301-315 of human IRS-1. |
detects IRS-1 only when phosphorylated at serine 307 (180kDa) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
WB |
Human |
Immunogen = phosphopeptide derived from a region of human IRS1 that contains tyrosine 1229 |
IRS1 when phosphorylated at Tyr 1229 |
- |
|
|
Abcam |
monoclonal
IgG |
Rabbit
(liquid, tissue culture supernatant, glycerol, BSA, NaCl, Tris glycine) |
WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide corresp. to residues near the C-terminus of human IRS1, phosphorylated at tyrosine 896 |
IRS1 when phosphorylated at Tyr 896 (170kDa) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, BSA, PBS, azide, affinity purified) |
WB |
Human, Mouse, Rat, African green monkey |
Immunogen = synthetic peptide: E S I T A T S P A S M V G G K, corresp. to aa301-315 of human IRS-1 |
recognizes IRS1 (~170 kDa) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, BSA, PBS, azide, affinity purified) |
WB |
Chicken, Human, Mouse, Rat |
Immunogen = synthetic peptide: L H T D D G Y M P M S P G V, corresp. to aa602-615 of human IRS-1 |
recognizes IRS1 (~170 kDa) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, BSA, PBS, azide, affinity purified) |
WB |
Human, Mouse, Rat, Xenopus |
Immunogen = synthetic peptide: D Y M P M S P K S V S A P Q Q I, corresp. to aa 631-646 of human IRS 1 |
recognizes IRS1 (~170 kDa) |
- |
|
|
EMD Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, PBS, glycerol, affinity purified) |
IB, IP, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide (Y Y A S I N F Q K Q P E D R Q) corresp. to aa1221-1235 of rat insulin receptor substrate-1 (IRS-1), conjugated to KLH |
recognizes the ~160-185 kDa IRS-1 protein |
- |
|
|
EMD Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, undiluted serum, no preservative) |
IB, IP, WB |
Human, Hamster, Mouse, Rat |
Immunogen = recombinant protein containing aa1122-1321 of mouse IRS-2 fused to GST |
recognizes the 180 kDa IRS-2 protein |
- |
|
|
EMD Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, PhosphoDetect™) |
IB, WB |
Human |
Immunogen = synthetic phosphopeptide surrounding the Ser616 phosphorylation site of human IRS-1 |
recognizes the ~165-180 kDa IRS-1 protein phosphorylated at Ser616 |
- |
|
|
EMD Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, PhosphoDetect™) |
IB, WB |
Human, Mouse, Rat |
Immunogen = synthetic phosphopeptide (S I T A T pS P A S M V G G K) corresp. to aa301-315 surrounding the Ser307 phosphorylation site of mouse IRS-1 |
recognizes the ~165-180 kDa IRS-1 protein phosphorylated at Ser307 |
- |
|
|
EMD Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, BSA, azide, PhosphoDetect™) |
IB, WB |
Human, Rat |
Immunogen = synthetic phosphopeptide corresp. to aa surrounding the Tyr896 phosphorylation site of human IRS-1 |
recognizes the ~165-180 kDa IRS-1 protein phosphorylated at Tyr896 |
- |
|
|
EMD Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, PBS, PhosphoDetect™) |
IB, WB |
Human |
Immunogen = synthetic phosphopeptide [K K K S P G E pY V N I E F G (C)] corresp. to aa 891-902 surrounding the Tyr896 phosphorylation site of human insulin receptor substrate-1 (hIRS-1) |
recognizes IRS-1 protein phosphorylated at Tyr896 |
- |
|
|
AnaSpec |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, PBS, azide) |
ELISA, IP, WB |
Bovine, Pig, Human, Mouse, Rat |
immunogen = a synthetic peptide (ITATpSPAS) corresponding to amino acids 308-315 of human IRS-1 at the phosphorylated site of Serine 312 |
detects IRS-1 |
- |
|
|
AnaSpec |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, PBS, azide) |
ELISA, WB |
Human, Mouse, Rat |
immunogen = a synthetic peptide (YKASpSPAESS) corresponding to amino acids 727-736 of human IRS-2 at the phosphorylated site of Serine 731 |
detects IRS-2 phosSer731 |
- |
|
|
AnaSpec |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, PBS, azide) |
ELISA, WB |
Human |
immunogen = a synthetic peptide corresponding to human IRS-1 to pair the phosphorylated site of Serine 312 (TATSPAS) |
detects IRS-1 |
- |
|
|
AnaSpec |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, PBS, azide) |
ELISA, WB |
Human, Mouse, Rat |
immunogen = a synthetic peptide (YKASpSPAESS) corresponding to amino acids 727-736 of human IRS-2 at the phosphorylated site of Serine 731 |
detects IRS-2 phosSer731 |
- |
|
|
AnaSpec |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, PBS, azide) |
ELISA, WB |
Human |
immunogen = a synthetic peptide corresponding to amino acids 727-736 surrounding serine 731 (KASSPAE) of human IRS-2 |
detects IRS-2 |
- |
|
|
AnaSpec |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, PBS, azide) |
ELISA, IC, WB |
Human, Mouse |
immunogen = a 16 amino acid peptide from near the center of human IRS-1 |
detects IRS-1 |
- |
|
|
AnaSpec |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, PBS, azide) |
IH |
Human, Mouse, Rat |
immunogen = a a synthetic peptide corresponding to amino acids 1223-1242 of human IRS-1 protein |
detects IRS-1 |
- |
|
|
EMD Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, BSA, azide, PhosphoDetect™) |
IB, WB |
Human, Mouse, Rat |
Immunogen = synthetic phosphopeptide corresp. to aa surrounding the Tyr612 phosphorylation site of human IRS-1 |
recognizes the ~165-180 kDa IRS-1 protein phosphorylated at Tyr612 |
- |