|
|
Santa Cruz |
polyclonal
IgG |
Goat
(PBS, azide, gelatin, affinity purified) |
IF, WB |
Human, Mouse, Rat |
immunogen = a peptide mapping at the C-terminus of Sec8 of human origin |
Sec8 |
- |
|
|
AbD Serotec |
monoclonal
IgM |
Mouse
(lyophilized, purified) |
IH, WB |
Amphibian (various), Bovine, Pig, Human, Rat |
Immunogen = native, coated brain vesicles |
Clathrin; stains coated vesicles from various organs and tissues tested e.g. brain, mammary gland, ovaries. Reacts with cultured cell lines of HeLa, SV-40 and RVF-SMC |
- |
|
|
AbD Serotec |
monoclonal
IgM |
Mouse
(liquid, purified) |
IH, WB |
Human |
- |
Clathrin |
- |
|
|
Bethyl Labs |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, Tris, azide) |
IC, WB |
Human |
immunogen = synthetic peptide, which represented a portion of human SNX1 encoded within exon 15 |
detects Sorting Nexin 1 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, Tris, azide) |
IC, WB |
Human |
immunogen = synthetic peptide, which represented a portion of human SNX1 encoded within exon 15 |
detects Sorting Nexin 1 |
- |
|
|
Abcam |
polyclonal
IgY |
Chicken
(liquid, affinity purified, PBS, no preservative) |
WB |
Human |
immunogen = recomb. full length protein, corresp. to aa 1-435 of Human AP2M1 |
detects AP2, mu chain 1 |
- |
|
|
BD Transduction |
monoclonal
IgG1 |
Mouse
(aqueous, buffered, BSA, glycerol, azide, affinity purified) |
IF, IH, IP, WB |
Dog, Human, Mouse, Rat |
immunogen = mouse Adaptin γ, aa. 641-821 |
104 kDa adaptin γ |
- |
|
|
BD Transduction |
monoclonal
IgG1 |
Mouse
(aqueous, buffered, BSA, glycerol, azide, affinity purified) |
IF, IH, IP, WB |
Dog, Human, Mouse, Rat |
immunogen = Mouse Adaptin α [A] aa. 38-215 |
112 kDa adaptin α |
- |
|
|
Oxford Biomedical Research, Inc. |
monoclonal
IgG |
Mouse
(liquid, protein-A purified, Tris, NaCl) |
WB |
Human |
immunogen = human RAP |
specific for the 38 kDalow density lipoprotein receptor-associated protein (RAP). |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
ELISA, WB |
Human, Monkey, Mouse, Rat |
Immunogen = synthetic peptide corresp. to residues surrounding Ile67 of human Clathrin (KLH-coupled) |
detects endogenous levels of total Clathrin protein |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
ELISA, IC, IF, IP, WB |
Human, Monkey, Mouse, Rat |
Immunogen = synthetic peptide corresp. to residues surrounding Pro1663 of human Clathrin (KLH-coupled) |
detects endogenous levels of total Clathrin protein |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG2b, κ |
Mouse
(liquid, PBS, affinity purified) |
IF, IH, IP, WB |
Bovine, Human |
Immunogen = synthetic peptide derived from bovine clathrin light chain corresponding to the conserved region of the light chains (aa23-44) |
recognizes both bovine LCa and LCb |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgY |
Chicken
(liquid, PBS, no preservative, affinity purified) |
ELISA, IH, WB |
Human |
Immunogen = recombinant protein aa1-211 from human CLTB |
CLTA and CLTB |
- |
|
|
A B R - Thermo Scientific |
monoclonal
IgG2a |
Mouse
(ascites, PBS, azide, diluted) |
IF, IP, WB |
Amphibian (various), Bovine, Porcine, Primate (various), Chicken, Human, Mouse, Rat |
Immunogen = purified bovine brain adaptor complexes |
detects the products of both alpha-adaptin genes, alphaA and alphaC, as well as an alternatively spliced isoform of alphaA found in neurons; recognizes an ~100 kDa doublet representing the two isoforms of alpha-adaptin from pig brain extract |
- |
|
|
A B R - Thermo Scientific |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, purified) |
IF, IP, WB |
Bovine, Primate (various), Human, Hamster, Mouse, Rat |
Immunogen = purified adaptors |
detects the products of both alpha-adaptin genes, alphaA and alphaC, as well as an alternatively spliced isoform of alphaA found in neurons; recognizes an ~100 kDa doublet representing the two isoforms of alpha-adaptin in rat brain |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
IP, WB |
Human |
Immunogen = Clathrin HC corresp. to the globular N-terminal domain of human origin |
Clathrin heavy chain; not recommended for detection of native clathrin |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
IF, WB |
- |
Immunogen = sucrose purified clathrin coated vesicles of soybean origin |
Clathrin heavy chain |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, IF, IH, IP, WB |
Human, Mouse, Rat |
Immunogen = epitope mapping at the C-terminus of human Clathrin HC |
Clathrin heavy chain |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin) |
ChIP, ELISA, IF, IH, IP, WB |
Human, Mouse, Rat |
Immunogen = epitope corresp. to aa1321-1620 of Clathrin HC 1 |
Clathrin heavy chain |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, IF, IH, IP, WB |
Cow, Human, Mouse, Rat |
Immunogen = epitope mapping at the N-terminus of human Clathrin HC |
Clathrin heavy chain |
- |
|
|
Santa Cruz |
monoclonal
IgM |
Mouse
(liquid, PBS, azide, gelatin) |
IP, WB |
Human |
Immunogen = human Clathrin heavy chain |
Clathrin heavy chain |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
Various species, Human |
Immunogen = N-terminus of human Clathrin heavy chain |
Clathrin heavy chain |
- |
|
|
Santa Cruz |
monoclonal
IgG2b |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IH, IP, WB |
Zebra fish, Various species, Cow, Human, Mouse, Rat, Xenopus |
Immunogen = aa23-44 of the conserved regions of cow Clathrin light chain |
detection of both Clathrin LCa and LCb |
- |
|
|
Santa Cruz |
monoclonal
IgG2b |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
Various species |
Immunogen = aa23-44 of bovine Clathrin light chain |
Clathrin light chain |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin) |
ELISA, IF, WB |
Various species, Human, Mouse, Rat |
Immunogen = epitope mapping within an internal region of rat Clathrin LCB |
Clathrin light chain B and, to a lesser extent, light chain A |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin) |
ELISA, IF, WB |
Human, Mouse, Rat |
Immunogen = epitope mapping within an internal region of rat Clathrin LCB |
Clathrin light chain B and, to a lesser extent, light chain A |
- |
|
|
Santa Cruz |
monoclonal
IgG2b |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IH, IP, WB |
Zebra fish, Cow, Human, Mouse, Rat, Xenopus |
Immunogen = synthetic peptide corresponding to aa23-44 of cow Clathrin LC |
Clathrin light chain |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
Immunogen = epitope corresp. to aa51-105 mapping within an internal region of human Clathrin LCA |
Clathrin light chain A |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
Immunogen = epitope corresp. to aa41-100 mapping within an internal region of human Clathrin LCB |
Clathrin light chain B |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide) |
IC, IF, IH, IM, IP, WB |
Cow, Human, Mouse |
Immunogen = purified human brain clathrin heavy chain |
binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole serum, thimerosal) |
IC, IF, IH, WB |
Chicken, Zebra fish, Various species, Cow, Human, Mouse, Rat, Xenopus, C. elegans, Drosophila |
Immunogen = rat/human peptide sequence: K A G L L Q R A L E H F T D L Y D I K R, corresp. to aa619-638 of rat Clathrin heavy chain, conj. to diphtheria toxin |
clathrin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IC, IF, IH, IP, WB |
Chicken, Human, Hamster, Mouse, Rat |
Immunogen = synthetic peptide derived from within residues 1650 to the C-terminus of human Clathrin heavy chain conj. to KLH |
clathrin |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, PBS, no preservative, protein G purified) |
IC, IF, WB |
Nicotiana (tobacco), Human, Hamster, Arabidopsis thaliana |
Immunogen = sucrose purified clathrin coated vesicles (Soybean) |
clathrin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole serum, thimerosal) |
IH, WB |
Human, Rat |
Immunogen = synthetic peptide: K A G L L Q R A L E H F T D L Y D I K R, corresp. to aa619-638 of rat Clathrin heavy chain |
clathrin |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, PBS, Thimerosal, protein G purified) |
IC, IF, WB |
Cow, Human |
Immunogen = globular N-terminal domain of clathrin heavy chain |
reacts with clathrin heavy chains (CHC) |
- |
|
|
Abcam |
monoclonal
IgM |
Mouse
(liquid, PBS, BSA, azide, purified) |
IH, WB |
Various species |
Immunogen = coated vesicles (clathrin) of bovine brain |
clathrin heavy chains (CHC); detects coated vesicles from different organs and tissues; |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, PBS, BSA, azide, protein G purified) |
WB |
Human |
Immunogen = globular N-terminal domain of human clathrin heavy chain |
clathrin heavy chains (CHC); not native clathrin |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, no preservative, protein G purified) |
WB |
Human |
Immunogen = globular N-terminal domain of human clathrin heavy chain |
clathrin heavy chains (CHC); not native clathrin |
- |
|
|
Abcam |
monoclonal
IgM |
Mouse
(liquid, PBS, no preservative, protein A purified) |
WB |
Human |
Immunogen = human clathrin heavy chain |
clathrin heavy chains (CHC) |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, protein A purified) |
WB |
Cow, Human, Mouse, Rat |
Immunogen = full length native protein (purified) (Cow); epitope resides within aa207-264 of bovine CHC |
reacts with clathrin heavy chain (CHC17); no cross-reaction with the muscle isoform CHC22. |
- |
|
|
Abcam |
monoclonal
IgG2b |
Mouse
(liquid, PBS, BSA, azide, protein A purified) |
IC, IF, IH, IP, WB |
Cow, Human, Rat |
Immunogen = synthetic peptide: E E D P A A A F L A Q Q E S E I A G I E N D, corresp. to aa23-44 of the conserved regions of cow Clathrin light chain |
reacts with both LCa and LCb |
- |
|
|
Abcam |
monoclonal
IgG2b |
Mouse
(liquid, PBS, Thimerosal, protein A purified) |
IC, IF, IP, WB |
Mammal (various) |
Immunogen = synthetic peptide: E E D P A A A F L A Q Q E S E I A G I E N D, corresp. to aa23-44 of the conserved regions of cow Clathrin light chain |
Clathrin Light Chain (CLC) |
- |
|
|
Abcam |
monoclonal
IgG2b |
Mouse
(liquid, PBS, no preservative, protein A purified) |
IC, IF, IH, IP, WB |
Cow, Human, Rat |
Immunogen = synthetic peptide: E E D P A A A F L A Q Q E S E I A G I E N D, corresp. to aa23-44 of the conserved regions of cow Clathrin light chain |
reacts with both LCa and LCb |
- |
|
|
Abcam |
polyclonal
IgY |
Chicken
(liquid, PBS, no preservative, affinity purified) |
ELISA, WB |
Human |
Immunogen = full length human clathrin protein |
clathrin light chain |
- |
|
|
Abcam |
monoclonal
IgG2b |
Mouse
(liquid, PBS, azide, protein A purified) |
IC, IF, IP, WB |
Mammal (various) |
Immunogen = synthetic peptide, corresp. to aa23-44 of cow Clathrin light chain |
Clathrin Light Chains (CLC), LCa and LCb; not heavy chain binding |
- |
|
|
Abcam |
monoclonal
IgG2b |
Mouse
(liquid, PBS, BSA, azide, protein A purified, prediluted) |
IH |
Cow, Human |
Immunogen = synthetic peptide: E E D P A A A F L A Q Q E S E I A G I E N D, corresp. to amino acids 23-44 of Cow Clathrin light chain |
Clathrin Light Chain (CLC) |
- |
|
|
Abcam |
monoclonal
IgM |
Mouse
(liquid, PBS, BSA, azide, ammonium sulfate ppt) |
IC, IF, IH, WB |
Amphibian (various), Bovine, Human, Rat |
Immunogen = coated vesicles (clathrin) of bovine brain |
reacts with the clathrin heavy chain |
- |
|
|
Abcam |
polyclonal
IgG |
Goat
(liquid, whole serum, azide) |
IC |
Various species, Cow, Human |
Immunogen = full length native protein (purified) (cow) |
clathrin |
- |
|
|
Abcam |
monoclonal
IgG2a |
Mouse
(liquid, ascites, azide) |
IC, IF, WB |
Cow, Human, Rat |
Immunogen = purified adaptors |
reacts with the 105 and 110 alpha-subunits of adaptor complex AP2 |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide) |
Block, IF, IH, IP, WB |
Primate (various), Cow, Human, Hamster, Mouse, Rat |
Immunogen = purified adaptors |
detects assembly polypeptide 2 (AP2); recognizes the products of both α-adaptin genes, alpha A and alpha C as well as an alternatively spliced isoform of alpha A found in neurons |
- |
|
|
US Biological |
monoclonal
IgG2a |
Mouse
(liquid, ascites, PBS, azide) |
IF, IP, WB |
Amphibian (various), Bovine, Porcine, Primate (various), Chicken, Human, Mouse, Rat |
Immunogen = purified bovine brain adaptor complexes |
recognizes the products of both -adaptin genes, -A and -C as well as an alternatively spliced isoform of -A found in neurons. |
- |
|
|
US Biological |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide) |
Block, IF, IP, WB |
Bovine, Primate (various), Human, Mouse, Rat |
Immunogen = purified alpha-adaptors |
detects assembly polypeptide 2 (AP2) ; recognizes the products of both -adaptin genes, -A and -C as well as an alternatively spliced isoform of -A found in neurons. |
- |
|
|
US Biological |
monoclonal
IgG2a |
Mouse
(liquid, ascites, PBS, azide) |
IC, IP, WB |
Bovine, Chicken, Frog, Pig, Human, Monkey, Mouse, Rat |
Immunogen = purified adaptors |
recognizes the products of both -adaptin genes, -A and -C as well as an alternatively spliced isoform of -A found in neurons. |
- |
|
|
US Biological |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, purified) |
FA, IC, IP, WB |
Bovine, Human, Monkey, Mouse, Rat |
Immunogen = purified adaptors |
recognizes the products of both -adaptin genes, -A and -C as well as an alternatively spliced isoform of -A found in neurons. |
- |
|
|
Abcam |
monoclonal
IgG2b |
Mouse
(liquid, ascites, azide) |
EM, IC, IF, IP, WB |
Cow, Human |
Immunogen = full length protein (cow) |
reacts with polypeptides of approximately 100 kDa in bovine liver, human heart fibroblasts, and Madin-Darby bovine kidney cultured cells (MDBK), but not with any components in the 110-115 kDa range from these sources, or from rat pheochromocytoma cultured cells (PC12), neuroblastoma or astrocytes. |
- |
|
|
Abcam |
monoclonal
IgG2a |
Mouse
(liquid, ascites, PBS, azide) |
IC, IF, IP, WB |
Amphibian (various), Primate (various), Chicken, Cow, Pig, Human, Mouse, Rat |
Immunogen = purified bovine brain adaptor complexes |
detects α-Adaptin; recognizes the products of both α-adaptin genes, alpha A and alpha C as well as an alternatively spliced isoform of alpha A found in neurons |
- |
|
|
Abcam |
polyclonal
IgG |
Goat
(liquid, azide, PBS, affinity purified) |
EM, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide: C T A P T S K P S S A G G E L, corresp. to aa 646 - 659 of human AP1G1 |
recognizes a band representing AP1G1 at just below 97kD that is blocked by the immunising peptide in HeLa extract |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum, thimerosal) |
IH, IP, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide: I R L R E L I R T I R T A R T Q A E E R, corresp. to N-terminal aa4-23 of γ Adaptin |
gamma Adaptin |
- |
|
|
Abcam |
polyclonal
IgG1 |
Rabbit
(liquid, whole serum, thimerosal, filtered) |
IH, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide: K K G E I F E L K A E L N S D K K E K, corresp. to N-terminal aa10-29 of AP1 and 2 (β) |
β 1+2 Adaptin |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
IC, IF, WB |
Cow, Human, Rat |
Immunogen = AP2 adaptor from bovine brain |
β 1+2 Adaptin |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Goat
(liquid, azide, KPBS, affinity purified) |
ELISA, IF, IH, WB |
Dog, Chicken, Human, Mouse, Rat |
Immunogen = synthetic peptide |
aa 646-659 of gamma-adaptin (AP1G1) |
- |
|
|
Santa Cruz |
monoclonal
IgG2a |
Mouse
(liquid, PBS, gelatin, azide) |
IP, WB |
Human, Rat |
Immunogen = AP2 polypeptides from cow brain |
reacts with the 105 and 110 α-subunits of adaptor complex AP2 |
- |
|
|
Santa Cruz |
monoclonal
IgG2a |
Mouse
(liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
Immunogen = raised against aa678-977 of mouse Adaptin 2 |
α-Adaptin 2; may cross react with α-Adaptin 1 |
- |
|
|
Santa Cruz |
monoclonal
IgG2a |
Mouse
(liquid, PBS, gelatin, azide) |
IF, IP, WB |
Human, Mouse, Rat |
Immunogen = purified cow brain adaptor complexes |
detection α-Adaptin 1 and 2, as well as an alternatively spliced isoform of α-Adaptin 1 found in neurons |
- |
|
|
Santa Cruz |
monoclonal
IgG2a |
Mouse
(liquid, PBS, gelatin, azide) |
IF, IP, WB |
Cow, Human, Mouse, Rat |
Immunogen = purified cow brain adaptor complexes |
detection α-Adaptin 1 and 2, as well as an alternatively spliced isoform of α-Adaptin 1 found in neurons |
- |
|
|
Santa Cruz |
monoclonal
IgG2a |
Mouse
(liquid, PBS, gelatin, azide) |
ELISA, IF, IH, IP, WB |
Human, Mouse, Rat |
Immunogen = aa678-977 (deletion at 707-728) of α-Adaptin 2 |
detection α-Adaptin 1 and 2 |
- |
|
|
Santa Cruz |
monoclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
Immunogen = epitope corresp. to aa678-977 (deletion at 707-728) of mouse α-Adaptin 2 |
detection α-Adaptin 1 and 2 |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, gelatin, azide) |
IF, IP, WB |
Cow, Human, Rat |
Immunogen = β-Adaptin of cow origin |
β-Adaptin |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, gelatin, azide, affinity purified) |
ELISA, IF, IP, WB |
Mouse, Rat |
Immunogen = peptide mapping at the C-terminus of mouse α-Adaptin |
α-Adaptin 2; may cross-react with α-Adaptin 1 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, gelatin, azide, affinity purified) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
Immunogen = epitope mapping at the N-terminus of human β-Adaptin |
β-Adaptin |
- |
|
|
Santa Cruz |
monoclonal
IgG2a |
Mouse
(liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
Immunogen = aa650-949 of human β-Adaptin |
β-Adaptin |
- |
|
|
Santa Cruz |
monoclonal
IgG2b |
Mouse
(liquid, PBS, gelatin, azide) |
ELISA, IP, WB |
Cow, Human, Rat |
Immunogen = full length γ-Adaptin of cow origin |
γ-Adaptin (100kDa); no cross-reaction with any components in the 110-115 kDa Adaptins |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
Cow, Human, Rat |
Immunogen = epitope corresp. to aa650-949 mapping at the C-terminus of human β-Adaptin |
β-Adaptin |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, gelatin, azide, affinity purified) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
Immunogen = epitope mapping at the N-terminus of human γ1-Adaptin |
γ1-Adaptin |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide, affinity purified) |
ELISA, IF, IH, IP, WB |
Human, Mouse, Rat |
Immunogen = epitope corresp. to aa653-821 mapping at the C-terminus of mouse γ1-Adaptin |
γ1-Adaptin |
- |
|
|
US Biological |
monoclonal
IgM |
Mouse
(lyophilized, PBS, azide, ammonium sulfate ppt) |
IH, WB |
Amphibian (various), Bovine, Human, Rat |
Immunogen = coated vesicles (clathrin) of bovine brain |
reacts with the clathrin heavy chain |
- |
|
|
US Biological |
monoclonal
IgM |
Mouse
(lyophilized, BSA, PBS, azide, ammonium sulfate ppt) |
IH, WB |
Amphibian (various), Bovine, Human, Rat |
Immunogen = coated vesicles (clathrin) of bovine brain |
reacts with the clathrin heavy chain |
- |
|
|
US Biological |
monoclonal
IgM |
Mouse
(lyophilized, gel filtration) |
IH, WB |
Amphibian (various), Bovine, Porcine, Human, Rat |
Immunogen = coated vesicles (clathrin) of bovine brain |
reacts with the clathrin heavy chain |
- |
|
|
US Biological |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, purified) |
IC, IP, WB |
Bovine, Human, Mouse |
Immunogen = purified human brain clathrin (heavy chain) |
clathrin heavy chain; ~180kDa by WB |
- |
|
|
US Biological |
polyclonal
|
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
ELISA, WB |
Bovine, Human, Mouse |
Immunogen = synthetic peptide corresp. to residues surrounding IIe67 of human Clathrin (KLH-coupled) |
detects endogenous levels of total Clathrin protein |
- |
|
|
US Biological |
polyclonal
|
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
ELISA, IC, IF, IP, WB |
Bovine, Human, Mouse |
Immunogen = synthetic peptide corresp. to residues surrounding Pro1663 of human Clathrin (KLH-coupled) |
detects endogenous levels of total Clathrin protein |
- |
|
|
US Biological |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, BSA, azide, glycerol, protein G affinity purified) |
WB |
Human |
Immunogen = globular N-terminal domain of clathrin heavy chain |
clathrin heavy chain; not native clathrin |
- |
|
|
US Biological |
monoclonal
IgG2b, κ |
Mouse
(liquid, PBS, BSA, azide, glycerol, protein A affinity purified) |
IF, IH, IP, WB |
Bovine, Human |
Immunogen = synthetic peptide derived from bovine clathrin light chain (aa23-44). |
recognizes LCa and LCb (25-29kD). |
- |
|
|
US Biological |
polyclonal
IgY |
Chicken
(liquid, PBS, no preservative, glycerol free, affinity purified) |
ELISA, WB |
Human |
Immunogen = synthetic peptide corresp. to aa1-211 of human clathrin |
Clathrin, Light Chain, isoform a |
- |
|
|
Cell Signaling |
polyclonal
|
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
WB |
Bovine, Human, Monkey, Mouse, Rat |
Immunogen = synthetic peptide corresp. to residues surrounding Ile67 of human clathrin (KLH-coupled) |
detects endogenous levels of total clathrin protein |
- |
|
|
Cell Signaling |
polyclonal
|
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
IC, IF, IP, WB |
Bovine, Human, Monkey, Mouse, Rat |
Immunogen = synthetic peptide corresp. to residues surrounding Pro1663 of human Clathrin (KLH-coupled) |
detects endogenous levels of total clathrin protein |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgM |
Mouse
(lyophilized, PBS, BSA, azide, purified by gel filtration) |
IH, WB |
Amphibian (various), Bovine, Pig, Human, Rat |
Immunogen = coated vesicles (clathrin) of bovine brain |
reacts with the clathrin heavy chain |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(liquid, serum, PBS, BSA, azide, purified by gel filtration) |
WB |
Human, Rat |
Immunogen = synthetic peptide |
Clathrin light chain a and b variants (CLC); recognizes a doublet at ~35-40 kDa |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
IF, IH, IP, WB |
Human, Mouse, Rat |
Immunogen = amino acids1-145 representing full length Tom20 of human origin |
detectsTom20 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
ELISA, IF, IH, IP, WB |
Human, Mouse, Rat |
Immunogen = amino acids62-361 of Tom40 of human origin |
detectsTom40 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, gelatin, azide, affinity purified) |
ELISA, IF, WB |
Human, Mouse, Rat |
Immunogen = peptide mapping near the C-terminus of Tom70 of human origin |
detectsTom70 |
- |
|
|
Novus Biologicals |
monoclonal
IgG1 |
Mouse
(liquid, ascites, PBS, azide) |
IF, IP, WB |
Primate (various), Cow, Human, Mouse, Rat |
Immunogen = purified adaptors |
detects assembly polypeptide 2 (AP2); recognizes the products of both α-adaptin genes, alpha A and alpha C as well as an alternatively spliced isoform of alpha A found in neurons. |
- |
|
|
Novus Biologicals |
monoclonal
IgG2 |
Mouse
(liquid, ascites, PBS, azide) |
IF, IP, WB |
Amphibian (various), Primate (various), Chicken, Cow, Pig, Human, Mouse, Rat |
Immunogen = purified bovine brain adaptor complexes |
detects the products of both α-adaptin genes, alpha A and alpha C as well as an alternatively spliced isoform of alpha A found in neurons |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, BSA, azide) |
WB |
Human |
immunogen = recombinant protein corresp. to an internal region of human EEA1 |
EEA1 |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, BSA, azide) |
IF, IP, WB |
Human, Rat |
immunogen = recombinant protein corresp. aa82-1411 of human EEA1 |
EEA1 |
- |
|
|
BD Transduction |
monoclonal
IgG1 |
Mouse
(aqueous, buffered, BSA, azide, glycerol, affinity purified, FITC conj.) |
IF |
Dog, Chicken, Human, Rat |
immunogen = Human EEA1, aa. 3-281 |
180 kDa EEA1 |
- |
|
|
Abcam |
monoclonal
IgG2a, κ |
Mouse
(liquid, PBS, azide, protein G purified) |
WB |
Human, Mouse |
immunogen = synthetic peptide derived from within residues 1350 to the C-terminus of human EEA1 |
EEA1 (160 and 100kDa) |
- |