|
|
BD Pharmingen |
monoclonal
IgG2a,κ |
Mouse
(liquid, aqueous, buffered, azide, affinity purified) |
FC, IF, IH |
- |
immunogen = mouse PDGF Receptor α chain -human IgG1 fusion protein |
PDGFR α chain; binds to PDGF A and B chains |
- |
|
|
Biolegend |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, azide, affinity purified; conj. PE) |
FC |
Human |
immunogen = NIH-3T3 cells transfected with human PDGFRβ |
PDGFR β chain |
- |
|
|
Biolegend |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, azide, affinity purified, conj. Biotin) |
FC |
Human |
immunogen = NIH-3T3 cells transfected with human PDGFRα |
PDGFR α chain |
- |
|
|
Biolegend |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, azide, affinity purified, conj. PE) |
FC |
Human |
immunogen = NIH-3T3 cells transfected with human PDGFRα |
PDGFR α chain |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, purified Ig, phosphate buffer, glycerol) |
IH, IP |
Human, Mouse |
immunogen = human epidermal growth factor purified from urine |
reacts with EGF |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(liquid, PBS, stabilizers, Tween, ProClin, IHC Select®, ready to use) |
IH |
Human |
immunogen = synthetic peptide from C-terminus of human EGFR |
reacts with EGF receptor |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, purified Ig, phosphate buffer, saline, glycerol, azide) |
ELISA, IC, IH, WB |
Human |
immunogen = intracellular, activated EGFr |
Reacts strongly and specifically with the intracellular domain of the activated and phosphorylated EGFr; believed to recognize a phosphorylation-dependent conformational on the C-terminal domain |
- |
|
|
Upstate and Chemicon are Millipore |
polyclonal
|
Rabbit
(lyophilized powder) |
Neut, WB |
Mouse |
immunogen = receptor grade mouse EGF |
reacts with 6 kDa EGF |
- |
|
|
Upstate and Chemicon are Millipore |
monoclonal
IgG1,κ |
Mouse
(lyophilized, PBS, azide/PEG, sucrose) |
IP, WB |
Human, Mouse |
immunogen = peptide containing the Y1173 motif (NAE[pY1173]LRV) of the human EGF receptor |
recognizes the motif NAE[pY]LRV corresp. to the major auto phosphorylation site of the human EGF receptor; this antibody does not interact with the non- phosphorylated EGF receptor |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, azide) |
ELISA, FA, IP, RIA |
Human |
immunogen = native protein |
EGF (6kD) |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(lyophilized, affinity purified) |
ELISA, Neut, WB |
Human |
immunogen = recombinant protein |
EGF |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(lyophilized, affinity purified) |
IC, IP, Neut, WB |
Mouse |
- |
EGF (6kD) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG2b |
Mouse
(lyophilized, ion exchange chromatography) |
ELISA, IP, WB |
Human |
immunogen = recombinant protein |
EGF |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(lyophilized, unpurified serum) |
ELISA, IC, WB |
Human |
immunogen = synthetic peptide |
EGF; no reaction to IGF-1 (human), IGF-2 (rat), TGF-α (human), ACTH (human, 1-39), Calcitonin (human), PTH (bovine, 1-84), somatostatin and vasoactive intestinal peptide |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(lyophilized, purified) |
ELISA, IC, WB |
Human |
immunogen = synthetic peptide |
EGF; no reaction to IGF-1 (human), IGF-2 (rat), TGF-α (human), ACTH (human, 1-39), Calcitonin (human), PTH (bovine, 1-84), somatostatin and vasoactive intestinal peptide |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(purified) |
IC, WB |
Human |
immunogen = recombinant protein |
EGF; no reaction to TGF alpha, hIGF-1 and hIGF-2 |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, PBS) |
ELISA, FA, WB |
Human |
immunogen = recombinant human EGF protein |
EGF |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Goat
(lyophilized, affinity purified) |
ELISA, WB |
Mouse |
immunogen = recombinant mouse EGF protein |
EGF |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Goat
(lyophilized, affinity purified, conj. biotin) |
ELISA, WB |
Mouse |
immunogen = recombinant mouse EGF protein |
EGF |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgY |
Chicken
(liquid, PBS, no preservative, affinity purified) |
ELISA, WB |
Human |
immunogen = recombinant human EGF protein |
EGF |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Goat
(lyophilized, affinity purified, conj. biotin) |
ELISA, WB |
Mouse |
immunogen = recombinant mouse EGF protein |
EGF |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Goat
(lyophilized, affinity purified) |
ELISA, WB |
Mouse |
immunogen = recombinant mouse EGF protein |
EGF |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Goat
(lyophilized, affinity purified, conj. biotin) |
ELISA, WB |
Mouse |
immunogen = recombinant mouse EGF protein |
EGF |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(lyophilized, ion exchange chromatography, conj. biotin) |
ELISA, WB |
Human |
immunogen = recombinant human EGF protein |
EGF |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Sheep
(lyophilized, iaffinity purified) |
ELISA, WB |
Rat |
immunogen = recombinant rat EGF protein |
EGF |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(liquid, supernatant) |
ELISA, WB |
Human |
immunogen = native protein |
EGF; no reaction to TGFα, hIGF-1 and hIGF-2 |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(liquid, supernatant) |
ELISA, WB |
Human |
immunogen = native protein |
EGF; no reaction to TGFα, hIGF-1 and hIGF-2 |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(liquid, purified) |
ELISA, WB |
Human |
immunogen = native protein |
EGF; no reaction to TGFα, hIGF-1 and hIGF-2 |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Goat
(lyophilized, affinity purified, conj. biotin) |
ELISA, WB |
Mouse |
immunogen = recombinant mouse EGF protein |
EGF |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Goat
(liquid, PBS) |
ELISA, WB |
Mouse |
immunogen = recombinant mouse EGF protein |
EGF |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Goat
(liquid, PBS, conj. Biotin) |
ELISA, WB |
Mouse |
immunogen = recombinant mouse EGF protein |
EGF |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, PBS, conj. biotin) |
ELISA, WB |
Human |
immunogen = recombinant human EGF protein |
EGF |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, affinity purified) |
ELISA |
Human |
immunogen = recombinant protein |
EGF (natural and recombinant); no reaction with TGFβ, IL-1B, IL-8, MCP-1 and SAA |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, affinity purified) |
ELISA |
Human |
immunogen = recombinant protein |
EGF (natural and recombinant); no reaction with TGFβ, IL-1B, IL-8, MCP-1 and SAA |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, affinity purified) |
ELISA |
Human |
immunogen = recombinant protein |
EGF (natural and recombinant); no reaction with TGFβ, IL-1B, IL-8, MCP-1 and SAA |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, affinity purified) |
ELISA |
Human |
immunogen = recombinant protein |
EGF (natural and recombinant); no reaction with other cytokines TGFβ, IL-1B, IL-8, MCP-1 and SAA |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, affinity purified) |
ELISA |
Human |
immunogen = recombinant protein |
EGF (natural and recombinant); no reaction with other cytokines TGFβ, IL-1B, IL-8, MCP-1 and SAA |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, glycerol, affinity purified) |
ELISA |
Human |
immunogen = recombinant protein |
EGF (natural and recombinant); no reaction with other cytokines TGFβ, IL-1B, IL-8, MCP-1 and SAA |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, thimerosal, glycerol, affinity purified) |
ELISA |
Human |
immunogen = recombinant protein |
EGF (natural and recombinant); no reaction with other cytokines TGFβ, IL-1B, IL-8, MCP-1 and SAA |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(protein G purified) |
ELISA |
Human |
immunogen = recombinant human EGF protein |
EGF (natural and recombinant) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, thimerosal, glycerol, affinity purified, conj. biotin) |
ELISA |
Human |
immunogen = recombinant protein |
EGF (natural and recombinant) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, azide, affinity purified) |
ELISA |
Human |
immunogen = recombinant protein |
EGF |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(lyophilized, affinity purified) |
ELISA |
Human |
immunogen = recombinant protein |
EGF (natural and recombinant) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, Tris-HCl, affinity purified) |
ELISA |
Human |
immunogen = recombinant protein |
recombinant EGF (oxidized or reduced form) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, Tris-HCl, affinity purified) |
ELISA |
Mouse |
immunogen = recombinant protein |
recombinant EGF (oxidized or reduced form) |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
|
ELISA |
Human |
- |
EGF |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(conj. biotin) |
ELISA |
Human |
immunogen = recombinant protein |
EGF |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(azide, no preservative) |
RIA |
Human, Mouse, Rat |
immunogen = native protein |
EGF native |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(no preservative) |
RIA |
Mouse |
immunogen = native protein |
EGF native |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(liquid, PBS, azide, affinity purified) |
IH |
Human |
immunogen = synthetic peptide C-terminal of human EGFR |
EGFR |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(liquid, PBS, azide, affinity purified) |
IH |
Human |
immunogen = synthetic peptide C-terminal of human EGFR |
EGFR |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(liquid, serum) |
ELISA, IH, IP, WB |
Human |
immunogen = synthetic peptide, C S L D N P D Y Q Q D F F P K E and corresp. to aa1166-1180 of the EGFR near the C-terminus identical in human, mouse and rat EGFR |
EGFR |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(liquid, KPBS, azide, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide aa1189-1199 of human EGFR |
EGFR |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(liquid, KPBS, azide, affinity purified) |
ELISA, IH, WB |
Human |
immunogen = synthetic peptide aa1189-1199 of human EGFR |
EGFR |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, BSA, azide, glycerol, affinity purified) |
ELISA, FC, IF, IH, IP, WB |
Human |
immunogen = HC2 20 d2 cells; epitope maps to the extracellular domain of EGFR |
EGFR; recognizes the 170kD (wild type) and 145kD (VIII variant) forms |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(liquid, PBS, no preservative, no glycerol, affinity purified) |
ELISA, FC, IC, IH, IP, WB |
Human, Mouse, Rat |
immunogen = synthetic phosphopeptide corresp. to residues surrounding Tyr1068 of human EGF receptor, KLH coupled |
detects endogenous levels of EGF receptor only when phosphorylated at Tyr1068; no cross reaction other sites of tyrosine phosphorylation on EGF receptor; no cross-reaction with other tyrosine-phosphorylated proteins |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Sheep
(liquid, PBS, no preservative, glycerol, protein G purified) |
ELISA, IC, IF, IP, WB |
Chicken, Human, Mouse, Rat |
immunogen = recombinant 20aa residues representing a portion of the internal domain of EGFR proximal to the phosphorylation region |
EGFR |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(liquid, HEPES, NaCl, BSA, no preservative, no glycerol, affinity purified) |
FC, IF, IH, IP, WB |
Human, Mouse, Rat |
immunogen = synthetic phosphopeptide corresp. to residues surrounding Tyr1068 of human EGF receptor, KLH coupled |
detects endogenous levels of EGF receptor only when phosphorylated at Tyr1068; no cross reaction with other proteins of the ErbB family |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, BSA, no preservative, no glycerol, affinity purified) |
ELISA, IC, IF, IH, WB |
Human |
immunogen = synthetic phosphopeptide corresp. to residues surrounding Tyr1045 of human EGF receptor, KLH coupled |
detects levels of EGF receptor only when phosphorylated at Tyr1045; may cross-react with other activated EGF receptor |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, BSA, no preservative, glycerol, affinity purified) |
ELISA, FC, IC, IF, IP, WB |
Human, Mouse, Rat |
immunogen = synthetic phosphopeptide corresp. to residues surrounding Tyr845 of human EGF receptor, KLH coupled |
detects endogenous levels of EGF receptor only when phosphorylated at Tyr845; may cross-react with other activated EGF receptor family members (e.g., ErbB2) but does not cross-react with other tyrosine-phosphorylated proteins |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG |
Mouse
(liquid, HEPES, NaCl, BSA, azide, glycerol) |
FC, IC, IH, IP, WB |
Human |
immunogen = synthetic phosphopeptide corresp. to residues surrounding Tyr1068 of human EGF receptor, KLH coupled |
detects overexpressed levels of EGF receptor only when phosphorylated at Tyr1068; no cross reaction with other sites of tyrosine phosphorylation on the EGFR; no cross-react with other tyrosine-phosphorylated proteins |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(lyophilized, affinity purified) |
IC, IH, IP, Neut, WB |
Human |
immunogen = human A431 membrane proteins |
recognizes the external domain of EGF receptor on all human cells. Competes with EGF and TGF-α for binding on human cells. Recognizes both phosphorylated and non-phosphorylated human EGF receptors |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, PBS, BSA, azide, glycerol, purified) |
ELISA, IC, IH, IP, WB |
Human |
immunogen = tyrosine-phosphorylated epidermal growth factor receptor |
reacts strongly and specifically with the intracellular domain of the activated and phosphorylated EGFR in human cell lines; no reaction with other phosphorylated proteins |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, NaCl, azide) |
FC, IH, IP, WB |
Human |
immunogen = A431 vulva epidermoid carcinoma cell line |
recognizes the EGF binding site of the extracellular domain of human EGFR (epidermal growth factor receptor; HER-1) |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, BSA, no preservative, glycerol, affinity purified) |
ELISA, IC, IF, IH, WB |
Human, Mouse, Rat |
immunogen = synthetic phosphopeptide corresp. to residues surrounding Tyr992 of human EGF receptor, KLH coupled |
detects levels of EGF receptor only when phosphorylated at Tyr992 (175kDa); no cross-reaction with other phosphorylated receptor tyrosine kinases |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG |
Rabbit
(liquid, Tris-glycine, NaCl, BSA, azide, glycerol) |
ELISA, IF, IH, IP, WB |
Human |
immunogen = synthetic peptide corresp. to the aa residues including and surrounding tyrosine 1173 of the human EGF Receptor (EGFR) |
detects levels of EGF receptor only when phosphorylated at Tyr1173 |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG2a |
Rat
(liquid, PBS, ion exchange chromatography) |
ELISA, FC, IH, IP, WB |
Human |
immunogen = cells |
EGF receptor |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG2b, κ |
Mouse
(liquid, PBS, BSA, azide, affinity purified) |
ELISA, FC, IF, IH |
Equine, Human |
immunogen = A431 human epidermoid cells |
EGFR (170kD) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, PBS, BSA, azide, glycerol, affinity purified) |
IH, IP, WB |
Human |
immunogen = extracellular domain of human recombinant EGFR protein |
recognizes Epidermal Growth Factor Receptor (erbB, EGFR) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, PBS, glycerol, protein G purified) |
IH, IP, WB |
Human |
immunogen = extracellular domain of human recombinant EGFR protein; MW of antigen:170kD (wild type) and 145kD (vIII variant) |
recombinant EGFR protein; blocks the binding of EGF to EGFR |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IH, IP, WB |
Human |
immunogen = synthetic peptide from the extracellular domain of human EGFR; MW of antigen: Wild Type: 170kD, vIII Variant: 145kD |
Epidermal Growth Factor Receptor (erbB, EGFR) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG2a |
Rat
(liquid, PBS, azide, ion exchange chromatography) |
FC, IH, IP, WB |
Human |
immunogen = extracellular domain of human EGF-receptor from head and neck carcinoma |
Epidermal Growth Factor Receptor (erbB, EGFR) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(lyophilized, purified, conj. PE) |
ELISA, IC, IP, WB |
Dog, Human, Mouse |
immunogen = synthetic peptide containing the Y1173 motif (...N A E [pY 1173 ] L R V...) of the human EGF receptor, KLH-conjugated |
recognizes the motif N A E [pY] L R V corresp. to the major auto-phosphorylation site of EGFR; Does not recognize the non-phosphorylated EGFR or other unrelated phosphotyrosine proteins |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(lyophilized, purified, conj. PE) |
ELISA, IC, IP, WB |
Dog, Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to aa 1170-1176 (N A E pY L R V) of EGFR (epidermal growth factor receptor) phosphorylated at Tyr1173 |
recognizes the motif N A E [pY] L R V corresp. to the major auto-phosphorylation site of EGFR; Does not recognize the non-phosphorylated EGFR or other unrelated phosphotyrosine proteins |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(lyophilized, purified, conj. biotin) |
ELISA, IC, IP, WB |
Dog, Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to aa 1170-1176 (N A E pY L R V) of EGFR (epidermal growth factor receptor) phosphorylated at Tyr1173 |
recognizes the motif N A E [pY] L R V corresp. to the major auto-phosphorylation site of EGFR; Does not recognize the non-phosphorylated EGFR or other unrelated phosphotyrosine proteins |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(lyophilized, purified, conj. FITC) |
ELISA, IC, IP, WB |
Dog, Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to aa 1170-1176 (N A E pY L R V) of EGFR (epidermal growth factor receptor) phosphorylated at Tyr1173 |
recognizes the motif N A E [pY] L R V corresp. to the major auto-phosphorylation site of EGFR; Does not recognize the non-phosphorylated EGFR or other unrelated phosphotyrosine proteins |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
|
ELISA, IC, IP, WB |
Human, Mouse |
immunogen = synthetic peptide corresp. to aa 1170-1176 (N A E Y L R K) of EGFR (epidermal growth factor receptor) |
recognizes the motif N A E Y L R V corresp. to the major auto-phosphorylation site of EGFR; no cross-reaction with phosphorylated EGFR |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
|
ELISA, IC, IP, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to aa 1165-1185 of EGFR (epidermal growth factor receptor) |
recognizes the C-terminus (aa 1165-1186) of EGFR |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(liquid, HEPES, NaCl, BSA, no preservative, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
immunogen = synthetic phosphopeptide corresp. to residues surrounding Tyr1068 of human EGF receptor, KLH coupled |
detects endogenous levels of EGF receptor only when phosphorylated at Tyr1068; no cross reaction with other sites of tyrosine phosphorylation on EGFR; no cross-reaction with other tyrosine-phosphorylated proteins |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, sucrose, glycerol, conj. to PE) |
ELISA, IC, IP, WB |
Dog, Human, Mouse |
immunogen = synthetic peptide corresp. to a portion of human EGFR (epidermal growth factor receptor) phosphorylated at Ser1047 |
recognizes human EGFR phosphorylated at Ser1047; no cross-reaction with non-phosphorylated EGFR or other unrelated serine-phosphorylated proteins |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide) |
FC, IF, IH, IP |
Human |
immunogen = HC2 20 d2 cells |
EGFR |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Rabbit
(liquid, PBS, Tris-glycine, NaCl, BSA, glycerol, azide, purified) |
FC, IF, IP, WB |
Human |
immunogen = synthetic peptide corresp. to the C-terminal region of human EGF Receptor (EGFR) |
recognizes the C-terminal domain of EGFR and does not cross-react with any other ErbB family member |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Sheep
(liquid, protein G purified) |
IC, IP, WB |
Chicken, Human, Rat |
immunogen = 20 aa synthetic peptide of human EGF receptor; sequence is proximal to the phosphorylation region (near the N-terminal sequence) |
recognizes the internal domain of the receptor molecule and will block the phosphorylation but not EGF |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, BSA, azide, glycerol, affinity purified) |
ELISA, IP, WB |
Equine, Human |
immunogen = synthetic peptide corresp. to residues 985-996 (D V V D A D E Y L I P Q) of the human EGF-Receptor |
recognizes the 170kD (wild type) and 145kD (vIII variant) forms of human EGF receptor. The epitope maps to the cytoplasmic domain of EGFR. Reacts with both the unphosphorylated and phosphorylated form of EGFR. Does not react with erbB-2, erbB-3 or erbB-4. |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, BSA, azide, affinity purified) |
ELISA, IH, IP |
Equine, Human |
immunogen = synthetic peptide corresp. to residues 985-996 (D V V D A D E Y L I P Q) of the human EGF-Receptor |
recognizes the 170kD (wild type) and 145kD (vIII variant) forms of human EGF receptor. The epitope maps to the cytoplasmic domain of EGFR. Reacts with both the unphosphorylated and phosphorylated form of EGFR. Does not react with erbB-2, erbB-3 or erbB-4. |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Rat
(liquid, PBS, azide, ion exchange chromatography) |
IH, IP |
Human |
immunogen = squamous cell carcinoma |
EGFR |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG2b |
Rat
(liquid, PBS, azide, ion exchange chromatography) |
IH, IP |
Human |
immunogen = breast carcinoma cells |
EGFR; binds to epitope D |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG2a |
Mouse
(liquid, Tris-glycine, NaCl, azide, affinity purified) |
IC, IP, WB |
Human |
immunogen = A431 membrane proteins |
recognizes the sequence of Ala351-Asp364 of human EGF receptor, Mr 180kD |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
immunogen = synthetic phosphopeptide derived from the region of human EGFR that contains tyrosine 1173 |
EGFR phosphorylated at Tyr1173 |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Sheep
(liquid, PBS, azide, affinity purified) |
IH, WB |
Human, Rat |
immunogen = synthetic peptide (Arg-Glu-lle-Pro-Asp-Leu-Leu-Glu-Lys-Gly-Glu-Arg-Leu-Pro-Gln-Pro-Pro-lle-Cys) from the cytoplasmic domain of human c-erbB-2 (KLH) |
EGFR |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, PBS, BSA, azide, glycerol) |
ELISA, IP, WB |
Human |
immunogen = extracellular and cytoplasmic domains of recombinant human EGFR protein |
EGFR |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG2a |
Mouse
(liquid, PBS, BSA, azide, glycerol, affinity purified) |
ELISA, IP, WB |
Human |
immunogen = extracellular domain of human recombinant EGFR protein, purified |
EGFR; (Mr 170kD and 145kD, corresponding to the intact and vIII variant); no cross-reactivity with c-erbB-2, c-erbB-3 or c-erbB-4. Epitope maps to the extracellular domain of EGFR (cell membrane) |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
ELISA, IH, WB |
Human |
immunogen = synthetic peptide from C-terminus of human EGFR protein |
EGFR |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(lyophilized) |
ELISA, IP, WB |
Human, Mouse |
immunogen = synthetic peptide corresp. to EGFR (epidermal growth factor receptor) phosphorylated at Tyr1045, conj. to KLH |
detects EGF receptor only when phosphorylated at Tyr1045 |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(liquid, PBS, BSA, azide, purified) |
IH |
Human |
immunogen = synthetic peptide |
EGFR |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(liquid, PBS, BSA, azide) |
IH, IP, WB |
Human |
immunogen = recombinant protein |
extracellular domain of human recombinant EGFR protein |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(liquid, PBS, azide) |
ELISA, IP, WB |
Human, Mouse, Rat |
immunogen = synthetic protein |
synthetic peptide (A D E pY L I P) corresp. to the tyrosine phosphorylated site of 1016 of human EGFR |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG2a |
Rat
(liquid, PBS) |
FC, IH, IP |
Human |
immunogen = cells |
squamous cell carcinoma; binds to epitope C |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG2b |
Rat
(liquid, PBS, ion exchange chromatography) |
FC, IH, IP |
Human |
immunogen = cells |
Breast carcinoma cells; binds to epitope D |
- |