β-Actin (EP1123Y)
Abcam
monoclonal
IgG
Rabbit
(liquid, glycerol, supernatant, Tris-glycine saline, BSA, azide)
-
Human, Rat
immunogen = synthetic peptide corresp. to residues near the C-terminus of human β-Actin (cytoplasmic)
β-Actin (42kDa)
-
α-Actin, sarcomeric (Alpha Sr-1)
Abcam
monoclonal
IgM
Mouse
(liquid, supernatant, tris-HCl, azide)
-
Rabbit, Chicken, Human, Mouse, Rat
immunogen = purified rabbit striated muscle actin
labels α-skeletal (α-sarcomeric) and α-cardiac muscle actins; not actin from fibroblasts (α-smooth muscle and β-cytoplasmic actin), aorta (α-smooth muscle), or chicken gizzard (γ-smooth muscle)
-
β-Actin
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to 16 aa near the C-terminus of human β-Actin
β-Actin
-
β-Actin
Abcam
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, protein G purified)
-
Human
immunogen = recombinant full length protein, corresp. to aa 1-376 of human β-Actin
β-Actin
-
β-Actin
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
-
Cat, Dog, Rabbit, Chicken, Human, Guinea Pig, Mouse, Rat, Drosophila
immunogen = synthetic peptide: A P R A V F P S, corresp. to aa 26-33 of β-Actin
β-Actin
-
Actin, smooth muscle (B4)
Abcam
monoclonal
IgG1 , κ
Mouse
(liquid, ascites, azide)
-
Mammal (various), Chicken
immunogen = purified chicken full length native protein
reacts with enteric smooth muscle γ-actin; binds to α-actin skeletal, vascular and cardiac smooth muscle actin; not cytoplasmic β- or γ-actin
-
Actin, smooth muscle (HUC1-1)
Abcam
monoclonal
IgG2a , κ
Mouse
(liquid, unpurified bioractor fluid, azide)
-
Avian (various), Various vertebrate, Human
immunogen = purified human umbilical artery full length native protein
muscle isoactins; not cytoplasmic actins
-
α-Actin, smooth muscle
Abcam
monoclonal
IgG2b , κ
Mouse
(liquid, PBS, protein G purified)
-
Human
immunogen = recombinant full length protein, corresp. to aa1-378 of human α- smooth muscle Actin
α-smooth muscle actin
-
α-Actin, smooth muscle (1A4)
Abcam
monoclonal
IgG2a , κ
Mouse
(liquid, NaCl, green food dye, supernatant, azide, prediluted)
-
Human
immunogen = Actin isolated from human myocardium
α-smooth muscle actin of blood vessels and parenchymal tissue of intestine, testis and ovary;not fibroblasts (β- and γ-cytoplasmic), striated muscle (α-saccomeric), and myocardium (α-myocardial) actins
-
β-Actin phos Tyr53/55
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse
immunogen = synthesized phosphopeptide derived from human β-Actin around the phosphorylation site of tyrosine 53/55
endogenous levels of β-Actin only when phosphorylated at tyrosine 53/55
-
Actin, muscle (HHF35)
Abcam
monoclonal
IgG1 , κ
Mouse
(liquid, tris-borate saline, supernatant, azide)
-
Human
immunogen = SDS extracted protein fraction of human myocardium
muscle specific α and γ actin isomers; not β and non-smooth muscle γ isomers
-
Actin, skeletal muscle
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide derived from human Actin α 1
endogenous levels of total actin α 1 protein (skeletal muscle)
-
Actin, skeletal muscle
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
-
Rabbit, Cow, Sheep, Human, Guinea Pig, Mouse, Rat
immunogen = synthetic peptide of human N-terminal(unfortunately, the aa seq. is considered commercially sensitive)
skeletal muscle actin
-
Actin, smooth muscle
Abcam
polyclonal
IgG1 , κ
Rabbit
(liquid, PBS, azide, ammonium sulfate ppt.)
-
Chicken
immunogen = a preparation derived from chicken gizzard smooth muscle actin
all actin isoforms
-
Actin, smooth muscle
Abcam
polyclonal
IgG
Goat
(liquid, tris buffered saline, BSA, azide, affinity purified)
-
Dog, Human, Mouse, Rat
immunogen = synthetic peptide: E E E D S T A L V C, corresp. to N- terminal aa 3-12 of human smooth muscle Actin
smooth muscle actin
-
Actin, smooth muscle (CGA7)
Abcam
monoclonal
IgG2b
Mouse
(liquid, PBS, BSA, azide, protein A purified)
-
Human
immunogen = full length protein: Actin from chicken gizzard
Actin isotypes α and γ of smooth muscle cells and myoepithelium but not skeletal or cardiac muscle cells; not reactive with other mesenchymal and all epithelial cells. Labels leiomyosarcomas and leiomyomas but not neoplasms such as carcinoma or lymphoma
-
α-Actin, sarcomeric (5C5)
Abcam
monoclonal
IgM
Mouse
(liquid, ascites, azide)
-
Rabbit, Snake, Carp, Cow, Sheep, Human, Guinea Pig, Rat
immunogen = full length purified rabbit striated muscle actin
α-skeletal and α-cardiac muscle actins; not smooth muscle tissue
-
β-Actin
Abcam
polyclonal
IgY
Chicken
(liquid, PBS, affinity purified)
-
Rabbit, Chicken, Cow, Human, Mouse, Rat
immunogen = full length human β-Actin
β-Actin
-
α-Actin, sarcomeric (5C5)
Abcam
monoclonal
IgM
Mouse
(liquid, PBS, azide, prediluted)
-
Rabbit, Snake, Carp, Cow, Sheep, Human, Guinea Pig, Rat, Xenopus
immunogen = full length purified rabbit striated muscle actin
α-skeletal and α-cardiac muscle actins; not smooth muscle tissue
-
β-Actin (mAbcam 8226)
Abcam
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, azide, affinity purified, conj. FITC)
-
Dog, Rabbit, Chicken, Cow, Horse, Sheep, Pig, Human, Guinea Pig, Mouse, Rat, African green monkey, Chinese hamster
immunogen = synthetic peptide derived from within residues 1-100 of human β-Actin, conj. to KLH
β-Actin; no cross-reaction with adult cardiac, smooth, or skeletal muscle actin
-
Actin, skeletal muscle
Abcam
polyclonal
IgM, κ
Rabbit
(liquid, PBS, BSA, azide, affinity purified, prediluted)
-
Rabbit, Human, Guinea Pig, Rat
immunogen = synthetic peptide derived from N-terminal of human α-skeletal muscle isoform of actin; acetylated at the N-terminus
skeletal muscle actin; reacts with sarcomeric actins of normal tissues and neoplasms derived from such tissues (i.e. rhabdomyosarcomas); not smooth muscle actin
-
Actin, smooth muscle (alpha sm-1)
Abcam
monoclonal
IgG2a
Mouse
(liquid, supernatant, azide)
-
Human
immunogen = synthetic N- terminal decapeptide of α-smooth muscle isoform of actin
smooth muscle α-actin; smooth muscle cells in blood vessel walls, gut wall, myometrium and arrectores pili of skin also myoepithelial cells found in breast and salivary gland
-
β-Actin
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(liquid, ascites, azide)
-
Fish (various), Bovine, Cat, Dog, Porcine, Rabbit, Chicken, Carp, Sheep, Human, Guinea Pig, Mouse, Rat, Xenopus, Drosophila
immunogen = synthetic peptide corresp. to aa1-14 [D D D I A A L V I D N G S G L] of Xenopus laevis β-Actin (KLH coupled). Epitope: N-terminal of the beta isoform of actin
β-actin; no cross with adult cardiac or skeletal muscle or amoeba β-actin
-
β-Actin
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(liquid, ascites)
-
Human
immunogen = synthetic beta cytoplasmic actin N-terminal peptide (Ac-D D D I A A L V I D N G S G) coupled to KLH. Epitope: The N-terminal of the beta isoform of actin
β-actin
-
Actin, smooth muscle (CGA7)
Chemicon and Upstate are Millipore
monoclonal
IgG1
Mouse
(liquid, PBS, Tween 20, ProClin, purified)
-
Human
immunogen = chicken gizzard actin
smooth muscle actin; cytoplasmic staining found in vascular walls, muscularis mucosae and propria in the gastrointestinal tract, myoepithelial cells in the salivary gland and breast, perifollicular dendritic cells in the spleen and Kupffer cells in the liver
-
Actin-associated protein (2E4)
None
monoclonal
IgM
Mouse
(liquid, ascites)
-
Human, Rat, Chicken
immunogen = preparation of human blood platelet proteins eluted by ATP from an F-actin affinity column
43kDa actin-associated protein (kaptin); binds to polymerized actin in vitro and stains the leading edge of platelets, fibroblast lamellipodia, epithelial stereocilia and neurite growth cones.
Discontinued
Actin, muscle (HHF35)
None
monoclonal
IgG1
Mouse
(liquid, affinity purified)
-
Human
-
actin isotypes α and γ (42kD) of skeletal, cardiac and smooth muscle; nonreactive with other mesenchymal and all epithelial cells, with the exception of myo-epithelium; labels rhabdomyosarcomas, hemangiopericytomas, leiomyosarcomas and leiomyomas; no reaction with non-myogenic sarcomas
Discontinued
Actin, smooth muscle (CGA7)
None
monoclonal
IgG2b
Mouse
(liquid, affinity purified, PBS, azide)
-
Human
-
actin isotypes α and γ (42kD) of smooth muscle, but not skeletal or cardiac muscle cells; nonreactive with other mesenchymal and all epithelial cells, with the exception of myo-epithelium; labels leiomyosarcomas and leiomyomas; not carcinoma or lymphoma
Discontinued
Actin, smooth muscle (1A4)
BioLegend
monoclonal
IgG2a
Mouse
(liquid, supernatant, Tris/HCl, azide)
-
Cow, Human, Rat, Chicken
immunogen = N-terminal synthetic decapeptide of α-smooth muscle actin coupled to KLH
smooth muxcle actin, N-terminal region
Available from BioLegend
Actin, sarcomeric (alpha Sr-1)
BioLegend
monoclonal
IgM
Mouse
(liquid, supernatant, Tris/HCl, azide)
-
Human, Rabbit, Mouse, Rat, Chicken
immunogen = purified striated muscle actin from rabbit.
α-skeletal (α-sarcomeric) and α-cardiac muscle actins. No labelling with actin from fibroblasts (α- smooth muscle and β-cytoplasmic actin), aorta (α-smooth muscle actin), or chicken gizzard (γ-smooth muscle actin)
Available from BioLegend
Actin, smooth muscle (1A4)
None
monoclonal
IgG2a
Mouse
(liquid, ascites, PBS, BSA, azide)
-
Human
immunogen = α-smooth muscle actin N-terminal decapeptide
specific for the α-smooth muscle actin isoform
Discontinued
Actin, muscle (HHF35)
None
monoclonal
IgG1
Mouse
(liquid, supernatant, PBS, BSA, azide)
-
Human
immunogen = muscle actin purified from human myocardium
reacts with a 42 kDa protein specific for actin present in skeletal, cardiac and smooth muscle; stains tumors of smooth muscle (leiomyosarcomas, leimyomas) and skeletal muscle (rhabdomyosarcomas)
Discontinued
Actin
US Biological
polyclonal
IgG
Rabbit
(liquid, serum, azide)
-
Chicken, Rat
immunogen = chicken back muscle actin bound to KLH
actin
-
Actin (2Q1055)
US Biological
monoclonal
IgG1 , κ
Mouse
(liquid, ascites, azide)
-
Plant (various), Various animal
immunogen = purified chicken gizzard actin
specific for the N-terminal two-thirds of the actin molecule (possibly near residues 50-70 in primary sequence). Reacts with every actin form tested
-
Actin (3G1)
US Biological
monoclonal
IgM
Mouse
(liquid, ascites, thimerosal, glycerol)
-
Rabbit
immunogen = rabbit muscle actin
α-actin
-
Actin
US Biological
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, glycerol, azide, purified)
-
Chicken, Human
immunogen = actin isolated from chicken back muscle
reacts with α, β, γ and δ-actin; no crossreactivity with other cytoskeletal proteins
-
Actin (5F100)
US Biological
monoclonal
IgM
Mouse
(liquid, supernatant, azide)
-
Bovine, Rabbit, Human, Xenopus
immunogen = profilin actin complex from calf thymus
specific for skeletal and non-muscle actin isoforms (43kDa)
-
Actin (5F130)
US Biological
monoclonal
IgM
Mouse
(liquid, PBS, BSA, supernatant, azide)
-
Chicken, Human, Mouse, Rat
immunogen = chicken skeletal muscle cells
specific for cytoskeletal actin
-
Actin
US Biological
polyclonal
IgG
Sheep
(lyophilized, affinity purified)
-
Human, Mouse, Rat
immunogen = non-muscle actin purified from human platelets
actin
-
Actin Bundles (1.B.11)
US Biological
monoclonal
Mouse
(liquid, supernatant)
-
Human
immunogen = human actin bundles
antigen associated with actin bundles
-
Actin Bundles (2Q1054)
US Biological
monoclonal
IgM
Mouse
(liquid, PBS, azide, supernatant)
-
Human
immunogen = human actin bundles
antigen associated with actin bundles
-
Actin, pan (2Q1055)
US Biological
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, glycerol, azide, protein A purified)
-
-
immunogen = purified chicken gizzard actin. epitope: located in the N-terminal two thirds of the actin molecule, possibly near aa 50-70
recognizes all six isoforms of vertebrate actin, globular (G) and fillimentous (F) forms
-
Actin, cardiac (5F152)
US Biological
monoclonal
IgG1
Mouse
(lyophilized, PBS, BSA, azide, protein G purified)
-
Bovine, Rabbit, Chicken, Human
immunogen = synthetic N-terminus decapeptide of cardiac isoform of actin
recognizes fetal cardiac actin; it discriminates fetal (cardiac) α-actin from all other actin isoforms. Shows no crossreaction with other actin isoforms present in skeletal and smooth muscle
-
Actin, muscle (3F10)
US Biological
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, glycerol, azide, protein G purified)
-
Bovine, Dog, Equine, Porcine, Human, Mouse, Rat
immunogen = purified actin
recognizes α-smooth muscle as well as α-skeletal and α-cardiac (sarcomeric) isoforms of actin. Reacts with tumors arising from smooth muscle (leiomyosarcomas) as well as skeletal muscle (rhabdomyosarcomas)
-
Actin, muscle (0.N.3)
US Biological
monoclonal
IgG1 , κ
Mouse
(liquid, supernatant, azide)
-
Rabbit, Human, Rat
immunogen = extract of human myocardium
recognizes α-actin (42kDa) from skeletal, cardiac and smooth muscle and γ-actin from smooth muscle sources. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas). No reaction with β or non-smooth muscle γ-actin.
-
Actin, muscle (5G2)
US Biological
monoclonal
IgG1
Mouse
(liquid, supernatant, azide, glycerol)
-
Rabbit, Human, Rat
immunogen = SDS extract of human myocardium
recognizes α-actin (42kDa) from skeletal, cardiac and smooth muscle and γ-actin from smooth muscle sources. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas). No reaction with β or non-smooth muscle γ-actin.
-
Actin, sarcomeric (6A4)
US Biological
monoclonal
IgM
Mouse
(liquid ascites, PBS, BSA, azide, purified)
-
Rabbit, Frog, Snake, Sheep, Human, Guinea Pig, Rat
immunogen = purified rabbit striated muscle actin
sarcomeric actin
-
Actin, skeletal muscle
US Biological
polyclonal
Rabbit
(liquid ascites, PBS, BSA, azide)
-
Bovine, Rabbit, Frog, Sheep, Human, Guinea Pig, Rat
immunogen = synthetic peptide derived from N-terminus of α skeletal muscle isoform of actin
skeletal muscle actin
-
Actin, smooth muscle (2Q1058)
US Biological
monoclonal
IgG2
Mouse
(liquid ascites)
-
Rabbit, Chicken, Human, Monkey
immunogen = chicken gizzard actin
smooth muscle actin
-
Actin, smooth muscle (3H517)
US Biological
monoclonal
IgG2a
Mouse
(liquid ascites, PBS, BSA, azide)
-
Porcine, Human, Rat
immunogen = α-smooth muscle actin N-terminal decapeptide
α-smooth muscle actin
-
Actin, smooth muscle
US Biological
polyclonal
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
-
Bovine, Rabbit, Chicken, Baboon, Human, Mouse, Rat
immunogen = synthetic peptide from N-terminus of human α-smooth muscle isoform of actin
α-smooth muscle actin
-
Actin, smooth muscle (5E2)
US Biological
monoclonal
IgG1
Mouse
(liquid, PBS, affinity purified)
-
Human
immunogen = human ACTA2 (aa1-378) with GST Tag. MWt 67.21kD (with tag)
α-smooth muscle actin 2
-
Actin, smooth muscle (6k6)
US Biological
monoclonal
IgG
Rabbit
(liquid, supernatant, Tris-glycine saline, azide, BSA, glycerol)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresponding to N-terminus of human α--actin 3
α-smooth muscle actin 3
-
Actin, F (4i374)
US Biological
monoclonal
IgM
Mouse
(liquid, PBS, azide, glycerol, purified)
-
Rabbit, Human
immunogen = human monocytes and U937 cell line
filamentous actin; bind to the N-terminal region of actin but not to the extreme N-terminal 40 aa
-
Actin, F, capping protein α-1
US Biological
polyclonal
IgY
Chicken
(liquid, PBS, affinity purified)
-
Chicken, Human, Mouse, Rat
immunogen = synthetic peptide corresp. to aa 80-150
Recognizes human Actin F Capping Protein α-1
-
Actin, F (8.S2.1)
US Biological
monoclonal
IgM
Mouse
(liquid, PBS, azide, glycerol, purified)
-
Rabbit, Human
immunogen = human monocytes and U937 cell line
filamentous actin; bind to the N-terminal region of actin but not to the extreme N-terminal 40 aa
-
Actin, F, capping protein α-1
US Biological
polyclonal
IgY
Chicken
(liquid, PBS, affinity purified)
-
Human, Mouse, Rat
immunogen = full-length actin F capping protein α-1
Recognizes human Actin F Capping Protein α-1
-
Actin, muscle (3H521)
US Biological
monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide, supernatant)
-
Cat, Dog, Rabbit, Chicken, Human, Monkey, Guinea Pig, Mouse, Rat, Macaque
immunogen = muscle actin, purified from human myocardium
recognizes α-actin; not β-actin
-
Actin, skeletal muscle
US Biological
polyclonal
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified)
-
Bovine, Rabbit, Frog, Sheep, Human, Guinea Pig, Rat
immunogen = synthetic peptide derived from N-terminus of α skeletal muscle isoform of actin
skeletal muscle actin
-
α-Actin, sarcomeric (3H516)
US Biological
monoclonal
IgM
Mouse
(liquid, BSA, azide, purified)
-
Human
immunogen = purified rabbit skeletal muscle actin
recognizes α-skeletal and α-cardiac actins (the sarcomeric actins). No reactivity with smooth muscle actin is seen.
-
α-Actin, sarcomeric (2704i)
US Biological
monoclonal
IgM
Rabbit
(liquid, Tris-borate saline, azide, supernatant)
-
Bovine, Rabbit, Snake, Carp, Sheep, Human, Guinea Pig, Rat, Xenopus
immunogen = purified rabbit striated muscle actin
recognizes α-skeletal and α-cardiac muscle actins
-
α-Actin, smooth muscle (0.N.5)
US Biological
monoclonal
IgG2a , κ
Mouse
(liquid, ascites, PBS, BSA, azide, glycerol, protein A purified)
-
Rabbit, Chicken, Baboon, Human, Mouse, Rat
immunogen = N-terminal decapeptide of the α-smooth muscle isoform of actin; acetylated at the N-terminus
recognizes α-smooth muscle; no crossreaction with actin from fibroblasts (β-and γ-cytoplasmic), striated muscle (a-sarcomeric), and myocardium (a-myocardial)
-
α-Actin, smooth muscle (1.BB.760)
US Biological
monoclonal
IgG2a
Mouse
(lyophilized, PBS, BSA, azide, protein A purified)
-
Bovine, Equine, Chicken, Human, Mouse, Rat
immunogen = synthetic N-terminal decapeptide of the α-smooth muscle isoform of actin
recognizes α-smooth muscle isoform of actin (43kDa)
-
α-Actin, smooth muscle (2Q1017)
US Biological
monoclonal
IgG2a
Mouse
(liquid ascites, PBS, BSA, azide, protein A purified)
-
Bovine, Chicken, Human, Mouse, Rat
immunogen = synthetic N-terminal decapeptide of the α-smooth muscle isoform of actin
recognizes many types of α-smooth muscle cells, such as those present in vascular walls, intestinal muscularis mucosae and propria, myometrium, stroma of various tissues and is also positive for myoepithelial cells of various glands, notably salivary and mammary gland
-
α-Actin, smooth muscle (5F160)
US Biological
monoclonal
IgG2a
Mouse
(lyophilized, PBS, BSA, azide, protein A purified)
-
-
immunogen = synthetic N-terminal decapeptide of the α-smooth muscle isoform of actin
recognizes α-smooth muscle isoform of actin (43kDa)
-
α-Actin, smooth muscle (5F160)
US Biological
monoclonal
IgG2a
Mouse
(liquid, PBS, BSA, azide, protein A purified)
-
-
immunogen = synthetic N-terminal decapeptide of the α-smooth muscle isoform of actin
recognizes α-smooth muscle isoform of actin (43kDa)
-
α-Actin, smooth muscle (6A3)
US Biological
monoclonal
IgG2a
Mouse
(liquid, ascites, PBS, BSA, azide, protein A purified)
-
-
immunogen = Actin synthetic peptide
recognizes α-smooth muscle isoform of actin (43kDa); no cross reaction with skeletal or cardiac muscle actin, mesenchymal cells, epithelial cells, or other cytoskeletal components
-
β-Actin (2A2.1)
US Biological
monoclonal
IgG1
Mouse
(liquid, ascites, azide, glycerol)
-
Bovine, Cat, Dog, Porcine, Rabbit, Chicken, Carp, Goldfish, Sheep, Human, Guinea Pig, Mouse, Rat, Xenopus, Drosophila
immunogen = synthetic peptide corresponding to aa1-14 [D D D I A A L V I D N G S G L] of Xenopus laevis β-Actin (KLH)
recognizes β-actin; no crossreaction with adult cardiac or skeletal muscle or amoeba β-actin
-
β-Actin
US Biological
polyclonal
IgG
Rabbit
(liquid, tris-citrate, phosphate buffer, azide, glycerol, affinity purified)
-
Various species, Human, Monkey, Mouse, Rat, Drosophila
immunogen = synthetic peptide from the N-terminus of human β-Actin
β-actin
-
β-Actin
US Biological
polyclonal
IgY
Chicken
(liquid, PBS, no preservative, affinity purified)
-
Bovine, Rabbit, Chicken, Various species, Human, Mouse, Rat
immunogen = synthetic peptide corresp. to aa 1-375
β-actin
-
β-Actin
US Biological
polyclonal
IgY
Chicken
(liquid, PBS, affinity purified)
-
Bovine, Rabbit, Chicken, Human, Mouse, Rat
immunogen = synthetic peptide corresp. to aa 151-320
β-actin
-
β-Actin (5E3)
US Biological
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, affinity purified)
-
Human
immunogen = human β-actin (aa 1-376) with GST Tag; MW: 66.99kD (with tag)
β-actin
-
β-Actin (8A102)
US Biological
monoclonal
IgG2a
Mouse
(lyophilized, affinity purified)
-
Fish (various), Goat, Rabbit, Chicken, Cow, Pig, Human, Hamster, Mouse
immunogen = peptide (D D D I A A L V V D N G S G), conj. to KLH
β-actin
-
β-Actin
US Biological
polyclonal
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human
immunogen = synthetic peptide aa 359-368 of human β-Actin
β-actin
-
β-Actin (8C5)
US Biological
monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide)
-
-
-
β-actin
-
β-Actin (8A103)
US Biological
monoclonal
IgG2a
Mouse
(liquid, PBS, BSA, thimerosal, affinity purified)
-
Fish (various), Goat, Rabbit, Chicken, Cow, Pig, Human, Hamster, Mouse
immunogen = peptide (D D D I A A L V V D N G S G), conj. to KLH
β-actin
-
γ-Actin
US Biological
polyclonal
IgG
Sheep
(liquid, serum, glycerol)
-
Various species, Human, Mouse, Rat
immunogen = synthetic peptide corresp. to the N-terminus of γ-Actin
γ-actin
-
Actin, pan (3F12)
US Biological
monoclonal
IgG1 , κ
Chicken
(liquid, PBS, BSA, azide, protein G purified)
-
Bovine, Dog, Porcine, Rabbit, Chicken, Human, Mouse, Rat, Slime molds, Dictyostelium
immunogen = chicken gizzard actin
recognizes all six isoforms of vertebrate actin, and two cytoplasmic actins (β, γ)
-
Actin (C4)
MP Biomedicals
monoclonal
IgG1 , κ
Mouse
(liquid, filtered ascites)
-
Non-specific
immunogen = chicken gizzard actin
recognizes an epitope that has been conserved in all actins ranging from human skeletal muscle to plants
-
Actin (C4/actin)
BD Transduction
monoclonal
Ig1 ,κ
Mouse
(supernatant or ascites, affinity purified, Alexa Fluor® 555 conj.)
-
Dog, Chicken, Human, Mouse, Rat
immunogen = chicken gizzard muscle Actin
reacts with all known isoforms of actin in vertebrate muscle and non-muscle cells
-
Actin (C4/actin)
BD Transduction
monoclonal
Ig1 ,κ
Mouse
(aqueous buffered solution, affinity purified, BSA, glycerol, azide)
-
Dog, Chicken, Human, Mouse, Rat
immunogen = chicken gizzard muscle Actin
reacts with all known isoforms of actin in vertebrate muscle and non-muscle cells; 42 kDa actin by WB
-
Actin-Cy3 (AC-40)
Sigma Aldrich
monoclonal
IgG2a
Mouse
(PBS, BSA, azide, purified)
-
Bovine, Dog, Goat, Rabbit, Chicken, Viper, Carp, Snail, Sheep, Pig, Human, Guinea Pig, Hamster, Mouse, Rat, Xenopus
immunogen = synthetic actin CN-terminal peptide, Ser-Gly-Pro-Ser-Ile- Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone
42 kDa actin; recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms
-
Actin-FITC (AC-40)
Sigma Aldrich
monoclonal
IgG2a
Mouse
(buffered aqueous solution, purified Ig, FITC conj.)
-
Bovine, Dog, Goat, Rabbit, Chicken, Viper, Carp, Snail, Various species, Sheep, Pig, Human, Guinea Pig, Hamster, Mouse, Rat, Xenopus
immunogen = synthetic actin CN-terminal peptide, Ser-Gly-Pro-Ser-Ile- Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone
42 kDa actin; recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms
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Actin
Sigma Aldrich
polyclonal
Rabbit
(liquid, IgG fraction, PBS, azide)
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Chicken, Human, Mouse, Rat
immunogen = actin from chicken back
useful for studying the intracellular distribution of actin structures and their functions in the contractile system; by IB, cross-reactivity with other proteins may be observed
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Actin
Sigma Aldrich
polyclonal
Rabbit
(buffered aqueous solution, PBS, BSA, azide, affinity isolated)
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Chicken, Frog, Human, Mouse, Rat
immunogen = synthetic actin N-terminal nonapeptide, conj. to KLH
useful for studying 42 kDa actin
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β-Actin (4E8H3)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
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Human
immunogen = peptide mapping at the N-terminus of β-Actin of human origin
detects beta actin, 43 kDa
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β-Actin (AC-15)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
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Various species
immunogen = slightly modified synthetic peptide corresponding to β-cytoplasmic actin
detects beta actin; may cross-react with all six known isoforms of Actin, including two cytoplasmic forms (β, λ); no cross reaction with adult cardiac, smooth or skeletal muscle actin
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β-Actin (N-21)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin, glycerol, BSA, thimerosal)
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Rabbit, Human, Mouse
immunogen = peptide mapping at the N-terminus of β-Actin of human origin
detects beta actin, 43 kDa
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β-Actin (R-22)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin, glycerol, BSA, thimerosal)
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Human, Mouse, Rat
immunogen = peptide mapping near the C-terminus of β-Actin of rat origin
detects the 15kDa C-terminal fragment and full-length beta Actin, 43kDa
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γ-Actin (1-17)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
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Cow, Human, Mouse, Rat
immunogen = cytoplasmic γ-Actin from cow brain tissue
γ-Actin
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pan-Actin (ACTN05 (C4))
Novus Biologicals
monoclonal
IgG1 ,κ
Mouse
(liquid, protein-G purified, ascites, PBS, BSA, azide)
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Dog, Rabbit, Cow, Pig, Human, Mouse, Rat
immunogen = chicken gizzard Actin
reacts with all six known vertebrate isoforms of actin (MW 42kDa). It alsoreacts with two cytoplasmic actins (beta, lambda) which are highly homologous to oneanother but which differ from the muscle actins at about 25 amino acid residues
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α-Actin
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, affinity purified, antisera, PBS, azide)
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Rabbit, Chicken, Cow, Baboon, Human, Mouse, Rat
immunogen = synthetic peptide corresponding to N-terminus of human alpha smooth muscle isoform of actin
stains smooth muscle cells in vessel walls, gut wall, and myometrium
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α-Actin, skeletal and muscle (5C5)
Sigma Aldrich
monoclonal
IgM
Mouse
(liquid, ascites, azide)
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Bovine, Rabbit, Frog, Snake, Sheep, Human, Guinea Pig, Rat
immunogen = rabbit striated muscle actin
specific for the single isoform of α-skeletal and α-cardiac muscle actins
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α-Actin, smooth muscle (1A4)
Sigma Aldrich
monoclonal
IgG2a
Mouse
(liquid, Tris, MgCl2 , azide, glycerol, BSA, purified)
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Bovine, Dog, Goat, Rabbit, Chicken, Frog, Snake, Sheep, Human, Guinea Pig, Mouse, Rat
immunogen = N-terminal synthetic decapeptide of α-smooth muscle actin
specific for the single isoform of α-smooth muscle actin (also known as anti-α-Sm-1)
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α-Actin, smooth muscle (1A4)
Sigma Aldrich
monoclonal
IgG2a
Mouse
(liquid, ascites, azide)
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Bovine, Dog, Goat, Rabbit, Chicken, Frog, Snake, Sheep, Human, Guinea Pig, Mouse, Rat
immunogen = N-terminal synthetic decapeptide of α-smooth muscle actin
specific for the single isoform of α-smooth muscle actin (also known as anti-α-Sm-1)
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α-Actin, smooth muscle, FITC (1A4)
Sigma Aldrich
monoclonal
IgG2a
Mouse
(liquid, PBS, BSA, azide, purified, conj. FITC)
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Bovine, Dog, Goat, Rabbit, Chicken, Frog, Snake, Sheep, Human, Guinea Pig, Mouse, Rat
immunogen = N-terminal synthetic decapeptide of α-smooth muscle actin
specific for the single isoform of α-smooth muscle actin (also known as anti-α-Sm-1)
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Actin
DSHB U. Iowa
monoclonal
IgM
Mouse
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Chicken, Various species, Rat
immunogen = cytoskeletal proteins from chicken gizzards
reacts with ~43kDa actin
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Actin (10A5)
Chemicon and Upstate are Millipore
monoclonal
IgM
Mouse
(liquid, ascites, azide)
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Human
immunogen = human actin
recognizes actin
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Actin (420-8)
Chemicon and Upstate are Millipore
monoclonal
IgM
Mouse
(liquid, PBS, supernatant, azide)
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Human
immunogen = chicken back muscle actin bound to KLH
recognizes actin bundles in human fibroblasts and epithelial cells; not Lymphoid cells
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Actin (C4)
Chemicon and Upstate are Millipore
monoclonal
IgG1 κ
Mouse
(liquid, purified, phosphate buffer, azide)
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Non-specific
immunogen = purified chicken gizzard actin (epitope near aa. 50-70)
recognizes all six isoforms of vertabrate actin; reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments
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