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Antibodies

511 Results

Name Source Clonality &
Isotype
Host &
Formulation
Method Reactivity Immunogen/
Epitope
Specificity Availability
Actin Related From manufacturers (100)
Abcam monoclonal
IgG
Rabbit
(liquid, glycerol, supernatant, Tris-glycine saline, BSA, azide)
IC, IF, IH, WB Human, Rat immunogen = synthetic peptide corresp. to residues near the C-terminus of human β-Actin (cytoplasmic) β-Actin (42kDa) -
Abcam monoclonal
IgM
Mouse
(liquid, supernatant, tris-HCl, azide)
IC, IH, WB Rabbit, Chicken, Human, Mouse, Rat immunogen = purified rabbit striated muscle actin labels α-skeletal (α-sarcomeric) and α-cardiac muscle actins; not actin from fibroblasts (α-smooth muscle and β-cytoplasmic actin), aorta (α-smooth muscle), or chicken gizzard (γ-smooth muscle) -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
IC, IF, WB Human, Mouse, Rat immunogen = synthetic peptide corresp. to 16 aa near the C-terminus of human β-Actin β-Actin -
Abcam monoclonal
IgG1, κ
Mouse
(liquid, PBS, protein G purified)
IH, WB Human immunogen = recombinant full length protein, corresp. to aa 1-376 of human β-Actin β-Actin -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
IH, WB Cat, Dog, Rabbit, Chicken, Human, Guinea Pig, Mouse, Rat, Drosophila immunogen = synthetic peptide: A P R A V F P S, corresp. to aa 26-33 of β-Actin β-Actin -
Abcam monoclonal
IgG1, κ
Mouse
(liquid, ascites, azide)
IC, IF, IH, WB Mammal (various), Chicken immunogen = purified chicken full length native protein reacts with enteric smooth muscle γ-actin; binds to α-actin skeletal, vascular and cardiac smooth muscle actin; not cytoplasmic β- or γ-actin -
Abcam monoclonal
IgG2a, κ
Mouse
(liquid, unpurified bioractor fluid, azide)
IC, IF, IH, WB Avian (various), Various vertebrate, Human immunogen = purified human umbilical artery full length native protein muscle isoactins; not cytoplasmic actins -
Abcam monoclonal
IgG2b, κ
Mouse
(liquid, PBS, protein G purified)
IC, IF, WB Human immunogen = recombinant full length protein, corresp. to aa1-378 of human α- smooth muscle Actin α-smooth muscle actin -
Abcam monoclonal
IgG2a, κ
Mouse
(liquid, NaCl, green food dye, supernatant, azide, prediluted)
IH, WB Human immunogen = Actin isolated from human myocardium α-smooth muscle actin of blood vessels and parenchymal tissue of intestine, testis and ovary;not fibroblasts (β- and γ-cytoplasmic), striated muscle (α-saccomeric), and myocardium (α-myocardial) actins -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
ELISA, IH Human, Mouse immunogen = synthesized phosphopeptide derived from human β-Actin around the phosphorylation site of tyrosine 53/55 endogenous levels of β-Actin only when phosphorylated at tyrosine 53/55 -
Abcam monoclonal
IgG1, κ
Mouse
(liquid, tris-borate saline, supernatant, azide)
IH Human immunogen = SDS extracted protein fraction of human myocardium muscle specific α and γ actin isomers; not β and non-smooth muscle γ isomers -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
ELISA, WB Human, Mouse, Rat immunogen = synthetic peptide derived from human Actin α 1 endogenous levels of total actin α 1 protein (skeletal muscle) -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
IH Rabbit, Cow, Sheep, Human, Guinea Pig, Mouse, Rat immunogen = synthetic peptide of human N-terminal(unfortunately, the aa seq. is considered commercially sensitive) skeletal muscle actin -
Abcam polyclonal
IgG1, κ
Rabbit
(liquid, PBS, azide, ammonium sulfate ppt.)
IC, IF, IP, WB Chicken immunogen = a preparation derived from chicken gizzard smooth muscle actin all actin isoforms -
Abcam polyclonal
IgG
Goat
(liquid, tris buffered saline, BSA, azide, affinity purified)
ELISA, WB Dog, Human, Mouse, Rat immunogen = synthetic peptide: E E E D S T A L V C, corresp. to N- terminal aa 3-12 of human smooth muscle Actin smooth muscle actin -
Abcam monoclonal
IgG2b
Mouse
(liquid, PBS, BSA, azide, protein A purified)
IH Human immunogen = full length protein: Actin from chicken gizzard Actin isotypes α and γ of smooth muscle cells and myoepithelium but not skeletal or cardiac muscle cells; not reactive with other mesenchymal and all epithelial cells. Labels leiomyosarcomas and leiomyomas but not neoplasms such as carcinoma or lymphoma -
Abcam monoclonal
IgM
Mouse
(liquid, ascites, azide)
IC, IF, IH Rabbit, Snake, Carp, Cow, Sheep, Human, Guinea Pig, Rat immunogen = full length purified rabbit striated muscle actin α-skeletal and α-cardiac muscle actins; not smooth muscle tissue -
Abcam polyclonal
IgY
Chicken
(liquid, PBS, affinity purified)
WB Rabbit, Chicken, Cow, Human, Mouse, Rat immunogen = full length human β-Actin β-Actin -
Abcam monoclonal
IgM
Mouse
(liquid, PBS, azide, prediluted)
IH Rabbit, Snake, Carp, Cow, Sheep, Human, Guinea Pig, Rat, Xenopus immunogen = full length purified rabbit striated muscle actin α-skeletal and α-cardiac muscle actins; not smooth muscle tissue -
Abcam monoclonal
IgG1, κ
Mouse
(liquid, PBS, azide, affinity purified, conj. FITC)
IC, IF Dog, Rabbit, Chicken, Cow, Horse, Sheep, Pig, Human, Guinea Pig, Mouse, Rat, African green monkey, Chinese hamster immunogen = synthetic peptide derived from within residues 1-100 of human β-Actin, conj. to KLH β-Actin; no cross-reaction with adult cardiac, smooth, or skeletal muscle actin -
Abcam polyclonal
IgM, κ
Rabbit
(liquid, PBS, BSA, azide, affinity purified, prediluted)
IH Rabbit, Human, Guinea Pig, Rat immunogen = synthetic peptide derived from N-terminal of human α-skeletal muscle isoform of actin; acetylated at the N-terminus skeletal muscle actin; reacts with sarcomeric actins of normal tissues and neoplasms derived from such tissues (i.e. rhabdomyosarcomas); not smooth muscle actin -
Abcam monoclonal
IgG2a
Mouse
(liquid, supernatant, azide)
IH Human immunogen = synthetic N- terminal decapeptide of α-smooth muscle isoform of actin smooth muscle α-actin; smooth muscle cells in blood vessel walls, gut wall, myometrium and arrectores pili of skin also myoepithelial cells found in breast and salivary gland -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(liquid, ascites, azide)
ELISA, IC, IF, IH, WB Fish (various), Bovine, Cat, Dog, Porcine, Rabbit, Chicken, Carp, Sheep, Human, Guinea Pig, Mouse, Rat, Xenopus, Drosophila immunogen = synthetic peptide corresp. to aa1-14 [D D D I A A L V I D N G S G L] of Xenopus laevis β-Actin (KLH coupled). Epitope: N-terminal of the beta isoform of actin β-actin; no cross with adult cardiac or skeletal muscle or amoeba β-actin -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(liquid, ascites)
ELISA, IF, IH, WB Human immunogen = synthetic beta cytoplasmic actin N-terminal peptide (Ac-D D D I A A L V I D N G S G) coupled to KLH. Epitope: The N-terminal of the beta isoform of actin β-actin -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, PBS, Tween 20, ProClin, purified)
IH Human immunogen = chicken gizzard actin smooth muscle actin; cytoplasmic staining found in vascular walls, muscularis mucosae and propria in the gastrointestinal tract, myoepithelial cells in the salivary gland and breast, perifollicular dendritic cells in the spleen and Kupffer cells in the liver -
US Biological polyclonal
IgG
Rabbit
(liquid, serum, azide)
IF, IH Chicken, Rat immunogen = chicken back muscle actin bound to KLH actin -
US Biological monoclonal
IgG1, κ
Mouse
(liquid, ascites, azide)
ELISA, IB, IF, WB Plant (various), Various animal immunogen = purified chicken gizzard actin specific for the N-terminal two-thirds of the actin molecule (possibly near residues 50-70 in primary sequence). Reacts with every actin form tested -
US Biological monoclonal
IgM
Mouse
(liquid, ascites, thimerosal, glycerol)
IH, WB Rabbit immunogen = rabbit muscle actin α-actin -
US Biological polyclonal
IgG
Rabbit
(liquid, PBS, BSA, glycerol, azide, purified)
ELISA, IH Chicken, Human immunogen = actin isolated from chicken back muscle reacts with α, β, γ and δ-actin; no crossreactivity with other cytoskeletal proteins -
US Biological monoclonal
IgM
Mouse
(liquid, supernatant, azide)
IH, WB Bovine, Rabbit, Human, Xenopus immunogen = profilin actin complex from calf thymus specific for skeletal and non-muscle actin isoforms (43kDa) -
US Biological monoclonal
IgM
Mouse
(liquid, PBS, BSA, supernatant, azide)
IC, IF, IH, WB Chicken, Human, Mouse, Rat immunogen = chicken skeletal muscle cells specific for cytoskeletal actin -
US Biological polyclonal
IgG
Sheep
(lyophilized, affinity purified)
WB Human, Mouse, Rat immunogen = non-muscle actin purified from human platelets actin -
US Biological monoclonal
Mouse
(liquid, supernatant)
IH Human immunogen = human actin bundles antigen associated with actin bundles -
US Biological monoclonal
IgM
Mouse
(liquid, PBS, azide, supernatant)
IF Human immunogen = human actin bundles antigen associated with actin bundles -
US Biological monoclonal
IgG1, κ
Mouse
(liquid, PBS, glycerol, azide, protein A purified)
ELISA, IB, IC, WB - immunogen = purified chicken gizzard actin. epitope: located in the N-terminal two thirds of the actin molecule, possibly near aa 50-70 recognizes all six isoforms of vertebrate actin, globular (G) and fillimentous (F) forms -
US Biological monoclonal
IgG1
Mouse
(lyophilized, PBS, BSA, azide, protein G purified)
ELISA, IB, IH, WB Bovine, Rabbit, Chicken, Human immunogen = synthetic N-terminus decapeptide of cardiac isoform of actin recognizes fetal cardiac actin; it discriminates fetal (cardiac) α-actin from all other actin isoforms. Shows no crossreaction with other actin isoforms present in skeletal and smooth muscle -
US Biological monoclonal
IgG1, κ
Mouse
(liquid, PBS, glycerol, azide, protein G purified)
IF, IH Bovine, Dog, Equine, Porcine, Human, Mouse, Rat immunogen = purified actin recognizes α-smooth muscle as well as α-skeletal and α-cardiac (sarcomeric) isoforms of actin. Reacts with tumors arising from smooth muscle (leiomyosarcomas) as well as skeletal muscle (rhabdomyosarcomas) -
US Biological monoclonal
IgG1, κ
Mouse
(liquid, supernatant, azide)
IH Rabbit, Human, Rat immunogen = extract of human myocardium recognizes α-actin (42kDa) from skeletal, cardiac and smooth muscle and γ-actin from smooth muscle sources. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas). No reaction with β or non-smooth muscle γ-actin. -
US Biological monoclonal
IgG1
Mouse
(liquid, supernatant, azide, glycerol)
IH Rabbit, Human, Rat immunogen = SDS extract of human myocardium recognizes α-actin (42kDa) from skeletal, cardiac and smooth muscle and γ-actin from smooth muscle sources. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas). No reaction with β or non-smooth muscle γ-actin. -
US Biological monoclonal
IgM
Mouse
(liquid ascites, PBS, BSA, azide, purified)
IH Rabbit, Frog, Snake, Sheep, Human, Guinea Pig, Rat immunogen = purified rabbit striated muscle actin sarcomeric actin -
US Biological polyclonal
Rabbit
(liquid ascites, PBS, BSA, azide)
IH Bovine, Rabbit, Frog, Sheep, Human, Guinea Pig, Rat immunogen = synthetic peptide derived from N-terminus of α skeletal muscle isoform of actin skeletal muscle actin -
US Biological monoclonal
IgG2
Mouse
(liquid ascites)
IH, WB Rabbit, Chicken, Human, Monkey immunogen = chicken gizzard actin smooth muscle actin -
US Biological monoclonal
IgG2a
Mouse
(liquid ascites, PBS, BSA, azide)
IH Porcine, Human, Rat immunogen = α-smooth muscle actin N-terminal decapeptide α-smooth muscle actin -
US Biological polyclonal
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
IH Bovine, Rabbit, Chicken, Baboon, Human, Mouse, Rat immunogen = synthetic peptide from N-terminus of human α-smooth muscle isoform of actin α-smooth muscle actin -
US Biological monoclonal
IgG1
Mouse
(liquid, PBS, affinity purified)
ELISA, WB Human immunogen = human ACTA2 (aa1-378) with GST Tag. MWt 67.21kD (with tag) α-smooth muscle actin 2 -
US Biological monoclonal
IgG
Rabbit
(liquid, supernatant, Tris-glycine saline, azide, BSA, glycerol)
FC, IH, IP, WB Human, Mouse, Rat immunogen = synthetic peptide corresponding to N-terminus of human α--actin 3 α-smooth muscle actin 3 -
US Biological monoclonal
IgM
Mouse
(liquid, PBS, azide, glycerol, purified)
ELISA, FC, IH, WB Rabbit, Human immunogen = human monocytes and U937 cell line filamentous actin; bind to the N-terminal region of actin but not to the extreme N-terminal 40 aa -
US Biological polyclonal
IgY
Chicken
(liquid, PBS, affinity purified)
ELISA, WB Chicken, Human, Mouse, Rat immunogen = synthetic peptide corresp. to aa 80-150 Recognizes human Actin F Capping Protein α-1 -
US Biological monoclonal
IgM
Mouse
(liquid, PBS, azide, glycerol, purified)
ELISA, FC, IH, WB Rabbit, Human immunogen = human monocytes and U937 cell line filamentous actin; bind to the N-terminal region of actin but not to the extreme N-terminal 40 aa -
US Biological polyclonal
IgY
Chicken
(liquid, PBS, affinity purified)
ELISA, WB Human, Mouse, Rat immunogen = full-length actin F capping protein α-1 Recognizes human Actin F Capping Protein α-1 -
US Biological monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide, supernatant)
IH, WB Cat, Dog, Rabbit, Chicken, Human, Monkey, Guinea Pig, Mouse, Rat, Macaque immunogen = muscle actin, purified from human myocardium recognizes α-actin; not β-actin -
US Biological polyclonal
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified)
IH Bovine, Rabbit, Frog, Sheep, Human, Guinea Pig, Rat immunogen = synthetic peptide derived from N-terminus of α skeletal muscle isoform of actin skeletal muscle actin -
US Biological monoclonal
IgM
Mouse
(liquid, BSA, azide, purified)
IH Human immunogen = purified rabbit skeletal muscle actin recognizes α-skeletal and α-cardiac actins (the sarcomeric actins). No reactivity with smooth muscle actin is seen. -
US Biological monoclonal
IgM
Rabbit
(liquid, Tris-borate saline, azide, supernatant)
IH Bovine, Rabbit, Snake, Carp, Sheep, Human, Guinea Pig, Rat, Xenopus immunogen = purified rabbit striated muscle actin recognizes α-skeletal and α-cardiac muscle actins -
US Biological monoclonal
IgG2a, κ
Mouse
(liquid, ascites, PBS, BSA, azide, glycerol, protein A purified)
IF, IH Rabbit, Chicken, Baboon, Human, Mouse, Rat immunogen = N-terminal decapeptide of the α-smooth muscle isoform of actin; acetylated at the N-terminus recognizes α-smooth muscle; no crossreaction with actin from fibroblasts (β-and γ-cytoplasmic), striated muscle (a-sarcomeric), and myocardium (a-myocardial) -
US Biological monoclonal
IgG2a
Mouse
(lyophilized, PBS, BSA, azide, protein A purified)
ELISA, IF, IH, WB Bovine, Equine, Chicken, Human, Mouse, Rat immunogen = synthetic N-terminal decapeptide of the α-smooth muscle isoform of actin recognizes α-smooth muscle isoform of actin (43kDa) -
US Biological monoclonal
IgG2a
Mouse
(liquid ascites, PBS, BSA, azide, protein A purified)
IH, WB Bovine, Chicken, Human, Mouse, Rat immunogen = synthetic N-terminal decapeptide of the α-smooth muscle isoform of actin recognizes many types of α-smooth muscle cells, such as those present in vascular walls, intestinal muscularis mucosae and propria, myometrium, stroma of various tissues and is also positive for myoepithelial cells of various glands, notably salivary and mammary gland -
US Biological monoclonal
IgG2a
Mouse
(lyophilized, PBS, BSA, azide, protein A purified)
IF, IH, WB - immunogen = synthetic N-terminal decapeptide of the α-smooth muscle isoform of actin recognizes α-smooth muscle isoform of actin (43kDa) -
US Biological monoclonal
IgG2a
Mouse
(liquid, PBS, BSA, azide, protein A purified)
IF, IH, WB - immunogen = synthetic N-terminal decapeptide of the α-smooth muscle isoform of actin recognizes α-smooth muscle isoform of actin (43kDa) -
US Biological monoclonal
IgG2a
Mouse
(liquid, ascites, PBS, BSA, azide, protein A purified)
IH - immunogen = Actin synthetic peptide recognizes α-smooth muscle isoform of actin (43kDa); no cross reaction with skeletal or cardiac muscle actin, mesenchymal cells, epithelial cells, or other cytoskeletal components -
US Biological monoclonal
IgG1
Mouse
(liquid, ascites, azide, glycerol)
ELISA, IC, IF, IH, WB Bovine, Cat, Dog, Porcine, Rabbit, Chicken, Carp, Goldfish, Sheep, Human, Guinea Pig, Mouse, Rat, Xenopus, Drosophila immunogen = synthetic peptide corresponding to aa1-14 [D D D I A A L V I D N G S G L] of Xenopus laevis β-Actin (KLH) recognizes β-actin; no crossreaction with adult cardiac or skeletal muscle or amoeba β-actin -
US Biological polyclonal
IgG
Rabbit
(liquid, tris-citrate, phosphate buffer, azide, glycerol, affinity purified)
IC, IF, IP, WB Various species, Human, Monkey, Mouse, Rat, Drosophila immunogen = synthetic peptide from the N-terminus of human β-Actin β-actin -
US Biological polyclonal
IgY
Chicken
(liquid, PBS, no preservative, affinity purified)
IC, IH, WB Bovine, Rabbit, Chicken, Various species, Human, Mouse, Rat immunogen = synthetic peptide corresp. to aa 1-375 β-actin -
US Biological polyclonal
IgY
Chicken
(liquid, PBS, affinity purified)
IH, WB Bovine, Rabbit, Chicken, Human, Mouse, Rat immunogen = synthetic peptide corresp. to aa 151-320 β-actin -
US Biological monoclonal
IgG1, κ
Mouse
(liquid, PBS, affinity purified)
ELISA, WB Human immunogen = human β-actin (aa 1-376) with GST Tag; MW: 66.99kD (with tag) β-actin -
US Biological monoclonal
IgG2a
Mouse
(lyophilized, affinity purified)
ELISA, WB Fish (various), Goat, Rabbit, Chicken, Cow, Pig, Human, Hamster, Mouse immunogen = peptide (D D D I A A L V V D N G S G), conj. to KLH β-actin -
US Biological polyclonal
Rabbit
(liquid, PBS, azide, affinity purified)
ELISA, IF, IH, WB Human immunogen = synthetic peptide aa 359-368 of human β-Actin β-actin -
US Biological monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide)
ELISA, FC, IC, WB - - β-actin -
US Biological monoclonal
IgG2a
Mouse
(liquid, PBS, BSA, thimerosal, affinity purified)
ELISA, WB Fish (various), Goat, Rabbit, Chicken, Cow, Pig, Human, Hamster, Mouse immunogen = peptide (D D D I A A L V V D N G S G), conj. to KLH β-actin -
US Biological polyclonal
IgG
Sheep
(liquid, serum, glycerol)
IC, IH, WB Various species, Human, Mouse, Rat immunogen = synthetic peptide corresp. to the N-terminus of γ-Actin γ-actin -
US Biological monoclonal
IgG1, κ
Chicken
(liquid, PBS, BSA, azide, protein G purified)
IH, IP, WB Bovine, Dog, Porcine, Rabbit, Chicken, Human, Mouse, Rat, Slime molds, Dictyostelium immunogen = chicken gizzard actin recognizes all six isoforms of vertebrate actin, and two cytoplasmic actins (β, γ) -
MP Biomedicals monoclonal
IgG1, κ
Mouse
(liquid, filtered ascites)
IB, IF Non-specific immunogen = chicken gizzard actin recognizes an epitope that has been conserved in all actins ranging from human skeletal muscle to plants -
BD Transduction monoclonal
Ig1
Mouse
(supernatant or ascites, affinity purified, Alexa Fluor® 555 conj.)
IF Dog, Chicken, Human, Mouse, Rat immunogen = chicken gizzard muscle Actin reacts with all known isoforms of actin in vertebrate muscle and non-muscle cells -
BD Transduction monoclonal
Ig1
Mouse
(aqueous buffered solution, affinity purified, BSA, glycerol, azide)
IF, WB Dog, Chicken, Human, Mouse, Rat immunogen = chicken gizzard muscle Actin reacts with all known isoforms of actin in vertebrate muscle and non-muscle cells; 42 kDa actin by WB -
Sigma Aldrich monoclonal
IgG2a
Mouse
(PBS, BSA, azide, purified)
IF Bovine, Dog, Goat, Rabbit, Chicken, Viper, Carp, Snail, Sheep, Pig, Human, Guinea Pig, Hamster, Mouse, Rat, Xenopus immunogen = synthetic actin CN-terminal peptide, Ser-Gly-Pro-Ser-Ile- Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone 42 kDa actin; recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms -
Sigma Aldrich monoclonal
IgG2a
Mouse
(buffered aqueous solution, purified Ig, FITC conj.)
IF Bovine, Dog, Goat, Rabbit, Chicken, Viper, Carp, Snail, Various species, Sheep, Pig, Human, Guinea Pig, Hamster, Mouse, Rat, Xenopus immunogen = synthetic actin CN-terminal peptide, Ser-Gly-Pro-Ser-Ile- Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone 42 kDa actin; recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms -
Sigma Aldrich polyclonal
Rabbit
(liquid, IgG fraction, PBS, azide)
IB, IF Chicken, Human, Mouse, Rat immunogen = actin from chicken back useful for studying the intracellular distribution of actin structures and their functions in the contractile system; by IB, cross-reactivity with other proteins may be observed -
Sigma Aldrich polyclonal
Rabbit
(buffered aqueous solution, PBS, BSA, azide, affinity isolated)
IB, IC, IH Chicken, Frog, Human, Mouse, Rat immunogen = synthetic actin N-terminal nonapeptide, conj. to KLH useful for studying 42 kDa actin -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
ELISA, FC, WB Human immunogen = peptide mapping at the N-terminus of β-Actin of human origin detects beta actin, 43 kDa -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
IF, WB Various species immunogen = slightly modified synthetic peptide corresponding to β-cytoplasmic actin detects beta actin; may cross-react with all six known isoforms of Actin, including two cytoplasmic forms (β, λ); no cross reaction with adult cardiac, smooth or skeletal muscle actin -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin, glycerol, BSA, thimerosal)
WB Rabbit, Human, Mouse immunogen = peptide mapping at the N-terminus of β-Actin of human origin detects beta actin, 43 kDa -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin, glycerol, BSA, thimerosal)
WB Human, Mouse, Rat immunogen = peptide mapping near the C-terminus of β-Actin of rat origin detects the 15kDa C-terminal fragment and full-length beta Actin, 43kDa -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
IF, IP, WB Cow, Human, Mouse, Rat immunogen = cytoplasmic γ-Actin from cow brain tissue γ-Actin -
Novus Biologicals monoclonal
IgG1
Mouse
(liquid, protein-G purified, ascites, PBS, BSA, azide)
IF, IH, IP, WB Dog, Rabbit, Cow, Pig, Human, Mouse, Rat immunogen = chicken gizzard Actin reacts with all six known vertebrate isoforms of actin (MW 42kDa). It alsoreacts with two cytoplasmic actins (beta, lambda) which are highly homologous to oneanother but which differ from the muscle actins at about 25 amino acid residues -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, affinity purified, antisera, PBS, azide)
IF, IH, WB Rabbit, Chicken, Cow, Baboon, Human, Mouse, Rat immunogen = synthetic peptide corresponding to N-terminus of human alpha smooth muscle isoform of actin stains smooth muscle cells in vessel walls, gut wall, and myometrium -
Sigma Aldrich monoclonal
IgM
Mouse
(liquid, ascites, azide)
ELISA, IB, IH Bovine, Rabbit, Frog, Snake, Sheep, Human, Guinea Pig, Rat immunogen = rabbit striated muscle actin specific for the single isoform of α-skeletal and α-cardiac muscle actins -
Sigma Aldrich monoclonal
IgG2a
Mouse
(liquid, Tris, MgCl2, azide, glycerol, BSA, purified)
IB, IH Bovine, Dog, Goat, Rabbit, Chicken, Frog, Snake, Sheep, Human, Guinea Pig, Mouse, Rat immunogen = N-terminal synthetic decapeptide of α-smooth muscle actin specific for the single isoform of α-smooth muscle actin (also known as anti-α-Sm-1) -
Sigma Aldrich monoclonal
IgG2a
Mouse
(liquid, ascites, azide)
ELISA, IB, IF, IH Bovine, Dog, Goat, Rabbit, Chicken, Frog, Snake, Sheep, Human, Guinea Pig, Mouse, Rat immunogen = N-terminal synthetic decapeptide of α-smooth muscle actin specific for the single isoform of α-smooth muscle actin (also known as anti-α-Sm-1) -
Sigma Aldrich monoclonal
IgG2a
Mouse
(liquid, PBS, BSA, azide, purified, conj. FITC)
IH Bovine, Dog, Goat, Rabbit, Chicken, Frog, Snake, Sheep, Human, Guinea Pig, Mouse, Rat immunogen = N-terminal synthetic decapeptide of α-smooth muscle actin specific for the single isoform of α-smooth muscle actin (also known as anti-α-Sm-1) -
DSHB U. Iowa monoclonal
IgM
Mouse
IP, WB Chicken, Various species, Rat immunogen = cytoskeletal proteins from chicken gizzards reacts with ~43kDa actin -
Chemicon and Upstate are Millipore monoclonal
IgM
Mouse
(liquid, ascites, azide)
ELISA, IC, IH, RIA, WB Human immunogen = human actin recognizes actin -
Chemicon and Upstate are Millipore monoclonal
IgM
Mouse
(liquid, PBS, supernatant, azide)
IF, IH Human immunogen = chicken back muscle actin bound to KLH recognizes actin bundles in human fibroblasts and epithelial cells; not Lymphoid cells -
Chemicon and Upstate are Millipore monoclonal
IgG1κ
Mouse
(liquid, purified, phosphate buffer, azide)
ELISA, IC, IH, WB Non-specific immunogen = purified chicken gizzard actin (epitope near aa. 50-70) recognizes all six isoforms of vertabrate actin; reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments -
Chemicon and Upstate are Millipore polyclonal
Rabbit
(liquid, serum, azide)
IF, IH Chicken, Rat immunogen = chicken back muscle actin bound to KLH recognizes actin -
A B R - Thermo Scientific polyclonal
Rabbit
(liquid, Tris, BSA, azide)
IP, WB Human, Mouse immunogen = N-terminus of human gamma actin using the numbering given in Swiss-prot entry P63261 and is highly conserved with beta actin detects beta and gamma Actin in human and mouse samples -
A B R - Thermo Scientific monoclonal
IgG1
Mouse
(liquid, PBS, glycerol, azide, protein-A purified)
ELISA, IC, IH, WB Various species immunogen = chicken gizzard actin detects Actin from a wide range of species samples; recongizes all six isoforms of vertebrate actin, globular and fillimentous forms -
Abcam polyclonal
IgG
Rabbit
(liquid, azide, affinity purified)
IC, IF, IH, WB Human, Mouse, Rat immunogen = synthetic peptide derived from within residues 1-100 of human α-smooth muscle Actin α-smooth muscle actin -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified, prediluted)
IH Rabbit, Chicken, Cow, Baboon, Human, Mouse, Rat immunogen = human synthetic peptide corresp. to N-terminus of smooth muscle α-actin α-smooth muscle actin -
A B R - Thermo Scientific polyclonal
IgG
Rabbit
(liquid, PBS, glycerol)
WB Human, Mouse, Rat immunogen = synthetic peptide, KLH-coupled, corresp. to N-terminal residues of human beta actin β-Actin -
A B R - Thermo Scientific polyclonal
Rabbit
(liquid, PBS, BSA, azide, affinity purified antisera)
IH, WB Cat, Dog, Rabbit, Chicken, Human, Guinea Pig, Mouse, Rat, Drosophila immunogen = synthetic peptide corresp. to residues A(26)P R A V F P S(33) of beta actin β-Actin -