|
|
Cell Signaling |
polyclonal
|
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
IH, IP, WB |
Human, Monkey, Mouse, Rat, Xenopus, Drosophila |
immunogen = synthetic peptide (KLH-coupled) corresp. to residues surrounding Asp244 of human beta-actin |
detects endogenous levels of total actin (all isoforms); also detected actin fragment (30kDa) cleaved at glutamate 107 |
- |
|
|
Cell Signaling |
monoclonal
|
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
FC, IC, IF, IH, WB |
Human, Monkey, Mouse, Rat |
immunogen = synthetic peptide (KLH-coupled) corresp. to residues near the N-terminus of human beta-actin |
detects endogenous levels of total β-actin; may cross react with γ-actin; no cross reaction with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms |
- |
|
|
Cell Signaling |
polyclonal
|
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
IH, IP, WB |
Bovine, Mink, Chicken, Human, Monkey, Hamster, Mouse, Rat, Xenopus |
immunogen = synthetic peptide (KLH-coupled) corresp. to residues near the N-terminus of human beta-actin |
detects endogenous levels of β-actin; may cross react with γ-actin; no cross reaction with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms |
- |
|
|
BD Pharmingen |
polyclonal
|
Rabbit
(aqueous buffered solution, azide) |
IF, IH, WB |
Human |
immunogen = human β-actin 240-245 Peptide |
recognizes the 32 kD cleaved fragment of human actin and does not recognize intact actin |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Chicken
(DEAE-column chromatography) |
IC, IH, WB |
Human |
immunogen = synthetic peptide corresp. to aa1-375 |
β-actin |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(affinity purified) |
IC, WB |
Human, Mouse, Rat |
immunogen = 16 aa peptide from near the N-terminus of human β-actin |
β-actin |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(liquid, ascites) |
IC, IF |
Bovine, Cat, Dog, Porcine, Rabbit, Chicken, Carp, Sheep, Human, Guinea Pig, Mouse, Rat, Xenopus |
immunogen = synthetic peptide(D D D I A A L V I D N G S G L) coupled to KLH, corresp. to aa 1-14 of Xenopus laevis beta isoform of actin |
β-actin; does not react with adult cardiac or skeletal muscle |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgY |
Chicken
(DEAE-column chromatography) |
IH, WB |
Bovine, Rabbit, Chicken, Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to aa 151-320 |
β-actin |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(protein G purified) |
WB |
Human, Mouse, Rat |
immunogen = aa 2-16 (C D D D I A A L V I D N G S G) of actin protein |
β-actin |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(DEAE-column chromatography) |
IC, IF, IP, WB |
Fish (various), Bovine, Cat, Dog, Rabbit, Chicken, Yeast, Sheep, Human, Mouse, Rat, Xenopus, Chinese hamster |
immunogen = synthetic peptide corresp. to aa 1-100 of human β-Actin |
β-actin; does not react with adult cardiac, smooth or skeletal muscle actin. |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(liquid, serum, no preservative) |
IC, IH, WB |
Human, Mouse, Rat |
immunogen = K-Y E L P D peptide representing the last five aa of the C-terminus of the 32 kDa Fractin fragment of beta-Actin |
32kDa Fractin fragment but not full length beta actin |
- |
|
|
A B R - Thermo Scientific |
monoclonal
IgG |
Mouse
(liquid, ascites, azide) |
ELISA, WB |
Bovine, Zebra fish, Arabidopsis, Yeast, Sheep, Human, Mouse, Rat, Xenopus, Drosophila |
immunogen = purified Arabidopsis actin protein |
actin |
- |
|
|
A B R - Thermo Scientific |
polyclonal
|
Rabbit
(liquid, Tris, BSA, azide, affinity purified antisera) |
IP, WB |
Human, Mouse |
immunogen = N-terminus of human beta actin using the numbering given in Swiss-prot entry P60709 and is highly conserved with gamma actin |
40 kDa Actin |
- |
|
|
A B R - Thermo Scientific |
polyclonal
IgG |
Rabbit
(liquid, prediluted) |
IH, IP |
Human |
immunogen = synthetic peptide corresponding to the N-terminal residues of human beta actin |
β-Actin |
- |
|
|
A B R - Thermo Scientific |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified IgG) |
WB |
Human |
immunogen = recomb., full length human beta Actin |
β-Actin |
- |
|
|
A B R - Thermo Scientific |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified IgG) |
IH |
Human |
immunogen = synthetic peptide corresponding to the N-terminal residues of human beta actin |
β-Actin |
- |
|
|
Sigma Aldrich |
polyclonal
IgG |
Rabbit
(liquid, IgG fraction, PBS, azide) |
IB, IF, IH |
Various species |
immunogen = N-terminal actin peptide (amino acid residues 20-33) attached to a multiple antigen peptide (MAP) backbone |
shows a broad reactivity among actin isoforms and across a range of organisms; specifically stains typical stress fibers in cultured cells using an indirect IF labeling technique and can be used as a probe of the N-terminal region of actin |
- |
|
|
Sigma Aldrich |
monoclonal
IgG2a |
Mouse
(liquid, ascites) |
ELISA, IC, IH, WB |
Various species |
immunogen = synthetic actin C-terminal peptide, Ser-Gly-Pro-Ser-Ile- Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone |
42 kDa actin; recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms |
- |
|
|
Rockland |
polyclonal
|
Rabbit
(liquid, antiserum, sterile filtered, affinity purified, phosphate buffer, saline, azide) |
ELISA, IH, WB |
Human |
- |
β-actin |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
FC, IF, IH, IP, WB |
Human, Mouse, Rat, Xenopus |
immunogen = peptide mapping at the C-terminus of human actin |
broad range of Actin isoforms |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ChIP, FC, IF, IP, WB |
Mammal (various), Dog, Zebra fish, Cow, Horse, Pig, Human, Mouse, Rat, Xenopus, C. elegans, S. cerevisiae |
immunogen = peptide mapping at the C-terminus of human actin |
broad range of Actin isoforms |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin, affinity purified) |
FC, IF, IP, WB |
Human, Mouse, Rat |
immunogen = aa180-375 mapping at the C-terminus of human actin |
broad range of Actin isoforms |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin) |
FC, IF, IP, WB |
Zebra fish, Human, Mouse, Rat, Xenopus, C. elegans, Drosophila |
immunogen = aa76-375 mapping at the C-terminus of human actin |
broad range of Actin isoforms |
- |
|
|
Santa Cruz |
monoclonal
IgM |
Mouse
(liquid, PBS, azide, gelatin) |
FC, IF, IP, WB |
Rabbit, Human, Mouse, Rat |
immunogen = monocytes and U-937 histolytic lymphoma of human origin |
Actin; no cross-reaction with the extreme N-terminal 40 aa |
- |
|
|
Santa Cruz |
monoclonal
IgM |
Mouse
(liquid, PBS, azide, gelatin) |
IF |
Human |
immunogen = K-562 human erythroleukemia cell line |
cytoplasmic Actin bundles |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IH, WB |
Chicken, Human, Mouse, Rat |
immunogen = purified chicken gizzard Actin |
Actin |
- |
|
|
Santa Cruz |
monoclonal
IgM |
Mouse
(liquid, PBS, azide, gelatin) |
FC, IF, IP, WB |
Human, Mouse, Rat |
immunogen = monocytes and U-937 histolytic lymphoma of human origin |
Actin; no cross-reaction with the N-terminal 40 aa |
- |
|
|
Santa Cruz |
monoclonal
IgM |
Mouse
(liquid, PBS, azide, gelatin) |
FC, IF, IP, WB |
Human, Mouse, Rat |
immunogen = monocytes and U-937 histolytic lymphoma of human origin |
Actin; no cross-reaction with the N-terminal 40 aa |
- |
|
|
Santa Cruz |
monoclonal
IgM |
Mouse
(liquid, supernatant, azide) |
IF, WB |
Chicken |
immunogen = skeletal muscle-cell preparation of chicken origin |
Actin |
- |
|
|
Santa Cruz |
monoclonal
IgM |
Mouse
(liquid, supernatant, azide) |
IF, WB |
Chicken |
immunogen = skeletal muscle-cell preparation of chicken origin |
Actin |
- |
|
|
Santa Cruz |
monoclonal
IgG2a |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IH |
Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to the N-terminal aa1-10 of human Actin |
Actin |
- |
|
|
Santa Cruz |
monoclonal
IgM |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IH |
Rabbit, Human, Mouse, Rat |
immunogen = purified rabbit actin |
α-Actin; not Actin from fibroblasts (α-smooth muscle and β-cytoplasmic), aorta (α-smooth mucle) or chicken gizzard (γ-smooth muscle) |
- |
|
|
Santa Cruz |
monoclonal
IgG2a |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
Human, Mouse, Rat |
immunogen = aa1-10 mapping at the N-terminus of smooth muscle α-Actin of human origin |
α-Actin; no cross reaction with Actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric) and myocardium (β-myocardial) |
- |
|
|
Santa Cruz |
monoclonal
IgG2a |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
Human, Mouse, Rat |
immunogen = aa1-10 mapping at the N-terminus of human smooth muscle α-Actin |
α-Actin (smooth muscle) |
- |
|
|
Santa Cruz |
monoclonal
IgG2a |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
Human, Mouse, Rat |
immunogen = aa1-10 mapping at the N-terminus of smooth muscle α-Actin of human origin |
α-Actin (smooth muscle) |
- |
|
|
Santa Cruz |
monoclonal
IgM |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IH, WB |
Rabbit, Human, Mouse, Rat, Xenopus |
- |
α-skeletal and α-cardiac Actin; not α-Actin from smooth muscle tissue |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IH, IP, WB |
Chicken, Human, Mouse, Rat |
immunogen = purified chicken gizzard Actin |
pan-Actin |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
Cow, Human, Mouse, Rat |
immunogen = cytoplasmic γ-Actin from cow brain tissue |
γ-Actin |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
Cow, Human, Mouse, Rat |
immunogen = cytoplasmic γ-Actin from cow brain tissue |
γ-Actin |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
Cow, Human, Mouse, Rat |
immunogen = cytoplasmic γ-Actin from cow brain tissue |
γ-Actin |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IH, IP, WB |
Mammal (various), Avian (various) |
immunogen = chicken gizzard actin |
α-smooth muscle, α-skeletal and α-cardiac (sarcomeric) isoforms of Actin; no cross-reaction with cytoplasmic actins |
- |
|
|
Santa Cruz |
monoclonal
IgG2b |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
Chicken, Human, Rat |
immunogen = chicken gizzard actin |
α- and γ- Actin isotypes of smooth muscle cells; no cross-reaction with skeletal or cardiac cells |
- |
|
|
Santa Cruz |
monoclonal
IgG2a |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IH, IP, WB |
Mammal (various), Avian (various) |
immunogen = human vascular muscle Actin |
all muscle actins; no cross-reaction with cytoplasmic actins |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IH, IP, WB |
Human |
immunogen = human myocardium Actin |
muscle α and γ actin isomers; no cross-reaction with β-actin isomers |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
IF, IP, WB |
Human, Mouse, Rat |
immunogen = peptide mapping near the C-terminus of human Arp2 |
actin related protein 2 (Arp2) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
IF, IP, WB |
Human, Mouse, Rat |
immunogen = peptide mapping near the N-terminus of human Arp2 |
actin related protein 2 (Arp2) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin, affinity purified) |
IF, IH, IP, WB |
Human, Mouse, Rat |
immunogen = peptide corresp. to aa 311-394 mapping at the C-terminus of Arp2 |
actin related protein 2 (Arp2) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
WB |
S. cerevisiae |
immunogen = peptide mapping near the N-terminus of Saccharomyces cerevisiae Arp2 |
actin related protein 2 (Arp2) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
WB |
S. cerevisiae |
immunogen = peptide mapping near the N-terminus of Saccharomyces cerevisiae Arp2 |
actin related protein 2 (Arp2) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
IF, IH, IP, WB |
Mammal (various), Cow, Human, Mouse, Rat |
immunogen = peptide mapping near the C-terminus of human Arp3 |
actin related protein 3 (Arp3) |
- |
|
|
Santa Cruz |
monoclonal
IgG2a |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
Human, Mouse, Rat |
immunogen = aa 1-110 of human Arp3 |
actin related protein 3 (Arp3) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
IF, IP, WB |
Mammal (various), Cow, Human, Mouse, Rat |
immunogen = peptide mapping near the N-terminus of human Arp3 |
actin related protein 3 (Arp3) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin) |
IF, IH, IP, WB |
Human, Mouse, Rat |
immunogen = aa 1-110 mapping at the N-terminus of human Arp3 |
actin related protein 3 (Arp3) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
S. cerevisiae |
immunogen = aa 1-152 peptide mapping at the N-terminus of Saccharomyces cerevisiae Arp3 |
actin related protein 3 (Arp3) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
IP, WB |
S. cerevisiae |
immunogen = peptide mapping at the N-terminus of Saccharomyces cerevisiae Arp3 |
actin related protein 3 (Arp3) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
WB |
S. cerevisiae |
immunogen = peptide mapping at the C-terminus of Saccharomyces cerevisiae Arp3 |
actin related protein 3 (Arp3) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
WB |
S. cerevisiae |
immunogen = peptide mapping at the N-terminus of Saccharomyces cerevisiae Arp9 |
actin related protein 9 (Arp9) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
WB |
S. cerevisiae |
immunogen = peptide mapping at the C-terminus of Saccharomyces cerevisiae Arp9 |
actin related protein 9 (Arp9) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
WB |
S. cerevisiae |
immunogen = peptide mapping at the N-terminus of Saccharomyces cerevisiae Arp7 |
actin related protein 7 (Arp7) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
WB |
S. cerevisiae |
immunogen = peptide mapping at the C-terminus of Saccharomyces cerevisiae Arp7 |
actin related protein 7 (Arp7) |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid,PBS, BSA, azide, protein G purified) |
IC, IF, IH, IP, WB |
Dog, Rabbit, Chicken, Cow, Pig, Human, Mouse, Rat, Dictyostelium |
immunogen = chicken gizzard actin |
reacts with all six known vertebrate isoforms of actin; also reacts with two cytoplasmic actins (β, λ) |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, ascites, azide) |
IC, IF, IH, WB |
Rabbit, Chicken, Cow, Sheep, Pig, Human, Mouse, Rat |
immunogen = chicken gizzard actin |
pan-actin |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, glycerol, azide, protein A purified) |
ELISA, IC, IH, WB |
Mammal (various), Avian (various), Bovine, Cat, Dog, Goat, Porcine, Rabbit, Rodent (various), Chicken, Frog, E. Coli, Protozoa, Yeast, Various species, Donkey, Horse, Sheep, Human, Guinea Pig, Hamster, Mouse, Rat, Xenopus, C. elegans, Macaque, Rhesus, Drosophila |
immunogen = chicken gizzard actin; Epitope appears to be located in the N-terminal two thirds of the actin molecule, possibly near aa 50-70 |
pan-actin that recognizes all six isoforms of vertebrate actin, globular (G) and fillimentous (F) forms; labels myotubes and stains myoblasts and fibroblasts. |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid,PBS, BSA, glycerol, azide) |
ELISA, IH |
Chicken, Human |
immunogen = actin isolated from chicken back muscle |
α actin; crossreacts with β, γ and δ actin; no crossreactivity with other cytoskeletal proteins |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified) |
ELISA, IH |
Human, Mouse |
immunogen = synthesized non phosphopeptide derived from human Actin pan (α/β/γ) around the phosphorylation site of tyrosine 53/55 |
endogenous levels of total actin protein |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified) |
ELISA, IH |
Human, Mouse, Rat |
immunogen = synthetic peptide derived from human Actin |
endogenous levels of total actin protein |
- |
|
|
Abcam |
monoclonal
IgG2b |
Rat
(liquid, PBS, azide, protein A purified) |
EM, IC, IF |
Rat, Drosophila |
immunogen = flight muscle extract from Lethocerus indicus (Waterbug) |
Actin in flight and non-flight muscles of Lethocerus and Drosophila; reacts with actin in flight muscles of all other insect species tested |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, BSA, azide, protein G purified) |
IC, IF, IH |
Dog, Horse, Pig, Human, Mouse, Rat |
immunogen = full length native protein (purified) |
reacts with α-smooth muscle, α-skeletal andα-cardiac (sarcomeric) isoform of actin. It is a pan muscle actin and reacts with tumors arising from smooth muscle (leiomyosarcomas) as well as skeletal muscle (rhabdomyosarcomas) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, ammonium sulfate ppt) |
IC, IF, WB |
Invertebrate (various), Chicken, Various vertebrate |
immunogen = purified full length native Actin protein from chicken gizzards |
reacts with F and G actins, from both muscle and non-muscle tissues |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, PBS, BSA, glycerol, azide, protein G purified) |
IC, IF, IH |
Dog, Chicken, Pig, Human, Mouse, Rat |
immunogen = full length purified human Actin |
reacts with α- smooth muscle as well as α-skeletal and α-cardiac (sarcomeric) isoform of actin. Reacts with tumors arising from smooth muscle (leiomyosarcomas) as well as skeletal muscle (rhabdomyosarcomas) |
- |
|
|
Abcam |
monoclonal
IgM |
Mouse
(liquid, ascites, thimerosal) |
IH, WB |
Rabbit, Human |
immunogen = rabbit muscle |
α-actin crossreactive with actin in eukaryotic cells including β, γ and δ no reactivity with other cytoskeletal components |
- |
|
|
Abcam |
monoclonal
IgG2a |
Mouse
(liquid, ascites, azide; for conj. PBS, BSA, azide, protein A or G purified) |
DB, ELISA, IC, ID, IF, IH, WB |
Dog, Goat, Rabbit, Chicken, Carp, Cow, Sheep, Pig, Human, Guinea Pig, Hamster, Mouse, Rat |
immunogen = synthetic peptide: S G P S I V H R K C F, corresp. to C- terminal aa 365-375 of human Actin, attached to Multiple Antigen Peptide (MAP) backbone |
epitope located on the C-terminal end of actin, conserved in all actin isoforms; no reaction with the epitope on the N-terminal end |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, ascites, azide) |
ID, WB |
Rabbit, Cow, Pig, Human |
immunogen = full length purified native cytosolic actin from Amoeba proteus |
actin epitope present in gizzard, skeletal and cardiac muscle |
- |
|
|
Abcam |
monoclonal
IgG2a, κ |
Mouse
(liquid, ascites, azide) |
IH, WB |
Rabbit, Snake, Cow, Sheep, Human, Guinea Pig, Rat |
immunogen = N-terminal decapeptide of α-smooth muscle actin for 1A4 and with purified rabbit striated muscle actin for 5C5 |
actin from muscle cells |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified) |
ELISA, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide derived from human α-sarcomeric Actin |
endogenous levels of total γ-Actin (45kDa) |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
DB, ELISA, IC, IF, IH, IM, WB |
Fish (various), Cat, Dog, Rabbit, Chicken, Cow, Opossum, Sheep, Pig, Human, Guinea Pig, Hamster, Mouse, Rat, Drosophila |
immunogen = synthetic peptide: D D D I A A L V I D N G S G L corresp. to aa1-14 of Xenopus laevis β-Actin, conj. to KLH |
β-Actin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
ELISA, IC, IF, IH, WB |
Chicken, Cow, Pig, Human, Mouse, Rat |
immunogen = human synthetic peptide corresp. to N-terminus of smooth muscle α-actin |
α-smooth muscle actin;not β- and γ-cytoplasmic, striated muscle, and myocardium actins |
- |
|
|
Abcam |
monoclonal
IgG2a, κ |
Mouse
(liquid, supernatant, tris-borate, NaCl, azide) |
ELISA, IC, IF, IH, WB |
Rabbit, Pig, Baboon, Human |
immunogen = N-terminal decapeptide of α-smooth muscle actin |
α-smooth muscle actin;not fibroblasts (β- and γ-cytoplasmic), striated muscle (α-saccomeric), and myocardium (α-myocardial) actins |
- |
|
|
Abcam |
monoclonal
IgG2a, κ |
Mouse
(liquid, affinity purified, conj. FITC) |
FC, IH, WB |
Zebra fish, Human |
immunogen = N-terminal decapeptide of α-smooth muscle actin |
α-smooth muscle actin;not fibroblasts (β- and γ-cytoplasmic), striated muscle (α-saccomeric), and myocardium (α-myocardial) actins |
- |
|
|
Abcam |
monoclonal
IgG2a, κ |
Mouse
(liquid, PBS, BSA, protien A affinity purified, conj. FITC) |
FC, IH, WB |
Rabbit, Zebra fish, Cow, Baboon, Human, Mouse, Rat |
immunogen = synthetic peptide: M C E E E D S T A L acetylated at the N-terminus, corresp. to aa 1-10 of α-smooth muscle Actin |
α-smooth muscle actin;not fibroblasts (β- and γ-cytoplasmic), striated muscle (α-saccomeric), and myocardium (α-myocardial) actins |
- |
|
|
Abcam |
monoclonal
IgG |
Rabbit
(liquid, Tris-glycine saline, BSA, glycerol, azide) |
FC, IH, IP, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide: corresp. to human N-terminus α-smooth muscle Actin |
α-smooth muscle actin |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, PBS, BSA, azide, conj. FITC) |
IF, IH |
Fish (various), Cat, Dog, Rabbit, Chicken, Cow, Sheep, Pig, Human, Guinea Pig, Mouse, Rat, Drosophila |
immunogen = synthetic peptide: D D D I A A L V I D N G S G L corresp. to aa1-14 of Xenopus laevis β-Actin, conj. to KLH |
β-Actin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, BSA, Tris citrate/phosphate, azide, affinity purified) |
IP, WB |
Human, Mouse |
immunogen = details of the immunogen are not available |
β and γ actin; epitope N-terminus of human γ actin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IC, IF, WB |
Fish (various), Dog, Rabbit, Chicken, Zebra fish, Cow, Sheep, Human, Mouse, Rat, Xenopus, Rhesus, Chinese hamster |
immunogen = synthetic peptide derived from within residues 1-100 of human β-Actin |
β-Actin |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, azide, affinity purified) |
FC, IC, IF, WB |
Dog, Rabbit, Chicken, Cow, Horse, Sheep, Pig, Human, Guinea Pig, Mouse, Rat, African green monkey, Chinese hamster |
immunogen = synthetic peptide derived from within residues 1-100 of human β-Actin |
β-Actin; not adult cardiac, smooth or skeletal muscle actin |
- |
|
|
Abcam |
monoclonal
IgG |
Mouse
(liquid, PBS, azide, affinity purified, conj. HRP) |
WB |
Dog, Rabbit, Chicken, Various species, Cow, Horse, Sheep, Pig, Human, Guinea Pig, Mouse, Rat, S. cerevisiae, African green monkey, Chinese hamster, Drosophila |
immunogen = synthetic peptide derived from within residues 1-100 of human β-Actin, conj. to KLH |
β-Actin |
- |
|
|
Abcam |
monoclonal
IgG3, κ |
Mouse
(liquid, Tris-HCl, NaCl, Na citrate. azide, protein A purified) |
WB |
Cat, Dog, Rabbit, Chicken, Cow, Pig, Human, Mouse, Rat, Xenopus, S. cerevisiae, S. pombe, Chinese hamster, Drosophila |
immunogen = synthetic peptide derived from within residues 1-100 of human β-Actin, conj. to KLH |
β-Actin (42kDa) |
- |
|
|
Abcam |
monoclonal
IgM |
Mouse
(liquid, PBS, azide, purified) |
ELISA, FC, IH, WB |
Rabbit, Human, Rat |
immunogen = human monocytes and U397 cell line; epitope = located between residues 120 and 226 |
filamentous actin (F-actin); binds to the N-terminal region of actin, but not to the extreme N-terminal 40 aa. Stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IC, IF, WB |
Chicken, Cow, Human, Mouse, Rat, Xenopus, Drosophila |
immunogen = synthetic peptide derived from within residues 350 to the C-terminus of human Actin, conj. to KLH |
β and λ actin; probably recognises all the other known forms of human actin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified, prediluted) |
IH |
Rabbit, Chicken, Cow, Baboon, Human, Mouse, Rat |
immunogen = synthetic peptide of human N-terminal(unfortunately, the aa seq. is considered commercially sensitive) |
smooth muscle actin |
- |
|
|
Abcam |
monoclonal
IgG |
Rabbit
(liquid, tris-glycine saline, supernatant, BSA, glycerol, azide) |
FC, IC, IF, IH, IP, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to residues near the N-terminus of human Actin |
muscle actin |
- |
|
|
Abcam |
monoclonal
IgG2a, κ |
Mouse
(liquid, PBS, BSA, azide, protein A purified) |
IH |
Rabbit, Chicken, Cow, Baboon, Human, Mouse, Rat |
immunogen = synthetic peptide: M C E E E D S T A L, corresp. to N-terminal aa 1-10 of α-smooth muscle actin |
α-smooth muscle actin;not fibroblasts (β- and γ-cytoplasmic), striated muscle (α-saccomeric), and myocardium (α-myocardial) actins |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, PBS, thimerosal, protein A purified, conj. HRP) |
WB |
Cat, Dog, Rabbit, Chicken, Frog, Carp, Sea Urchin, Various species, Cow, Sheep, Pig, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat, Xenopus, Drosophila |
immunogen = synthetic peptide: D D D I A A L V I D N G S G L corresp. 100% to a sequence from: Xenopus borealis (Kenyan clawed frog), Lytechinus pictus (Painted sea urchin) and Engystomops pustulosus (Tungara frog), conj. to KLH |
β-Actin; not adult cardiac and skeletal muscle β-Actin |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, tris-borate saline, supernatant, azide) |
IH |
Human |
immunogen = SDS extracted protein fraction of human myocardium |
muscle specific α and γ actin isomers; not β isomers |
- |
|
|
Abcam |
polyclonal
IgY |
Chicken
(liquid, PBS, affinity purified) |
IC, WB |
Rabbit, Chicken, Various species, Cow, Human, Mouse, Rat |
immunogen = aa 151-320 of human β-Actin |
β-Actin |
- |
|
|
Abcam |
polyclonal
IgY |
Chicken
(liquid, PBS, BSA, azide, affinity purified) |
IC, IF, WB |
Fish (various), Dog, Rabbit, Chicken, Cow, Human, Mouse, Rat, Xenopus, Chinese hamster |
immunogen = synthetic peptide derived from within residues 1-100 of human β-Actin |
β-Actin (40kDa); no cross-reaction with adult cardiac, smooth or skeletal muscle actin |
- |
|
|
Abcam |
monoclonal
IgG |
Mouse
(liquid, supernatant, tris-borate, NaCl, azide) |
IH, WB |
Human, Mouse, Rat |
immunogen = purified rabbit striated muscle actin |
α-skeletal and α-cardiac muscle actins; not smooth muscle tissue |
- |
|
|
Abcam |
polyclonal
IgG |
Goat
(liquid, PBS, azide, affinity purified) |
IC, IF, WB |
Fish (various), Dog, Rabbit, Chicken, Various species, Cow, Human, Monkey, Mouse, Rat, Chinese hamster |
immunogen = synthetic peptide derived from within residues 1-100 of human β-Actin, conj. to KLH |
β-Actin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, KPBS, azide, affinity purified) |
ELISA, IH, WB |
Various species |
immunogen = synthetic peptide: T F Q Q M W I S K Q, corresp. to aa 359-368 of β-Actin |
β-Actin |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide) |
EM, IC, IF, IH, WB |
Cat, Dog, Rabbit, Chicken, Carp, Cow, Sheep, Pig, Human, Guinea Pig, Mouse, Rat, Drosophila |
immunogen = synthetic peptide: D D D I A A L V I D N G S G L, conj. KLH |
β-Actin |
- |