|
|
Cell Signaling |
polyclonal
|
Rabbit
(liquid, affinity purified) |
WB |
Human, Monkey, Mouse, Rat |
immunogen = synthetic peptide (KLH-coupled) derived from residues surrounding Phe52 of human eIF4A |
detects endogenous levels of total eIF4A1, eIF4A2 and eIF4A3 proteins |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, affinity purified, PBS, azide, gelatin) |
WB |
S. cerevisiae |
immunogen = peptide mapping near the N-terminus of eIF4A of Saccharomyces cerevisiaeorigin |
detects eIF4A |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
Human, Mouse, Rat |
immunogen = amino acids186-330 mapping within an internal region of eIF4AI of human origin |
detects eIF4AI and eIF4AII |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, affinity purified, PBS, azide, gelatin) |
IF, WB |
Human, Mouse, Rat |
immunogen = peptide mapping at the N-terminus of eIF4AI of human origin |
detects eIF4AI |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, affinity purified, PBS, azide, gelatin) |
IF, IP, WB |
Human, Mouse, Rat |
immunogen = peptide mapping at the N-terminus of eIF4AII of human origin |
detects 46 kDa eIF4AII |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
Human |
immunogen = recombinantGST-tagged eIF4AIII protein of human origin |
detects eIF4AIII |
- |
|
|
Cell Signaling |
polyclonal
|
Rabbit
(liquid, affinity purified) |
IH, WB |
Zebra fish, Human, Monkey, Mouse, Rat |
immunogen = peptide (KLH coupled) corresp. to residues surrounding Ser209 of eIF4E |
detects endogenous levels of total eIF4E |
- |
|
|
Cell Signaling |
polyclonal
|
Rabbit
(liquid, protein-A and affinity purified, HEPES, saline, BSA, glycerol) |
WB |
Human, Monkey, Mouse, Rat |
immunogen = synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1 |
detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46; may cross-react with 4E-BP2 and 4E-BP3 when phosphor. at equivalent sites |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(liquid, protein-A and affinity purified, HEPES, saline, BSA, glycerol, azideAvailable as Alexa Fluor® 488 Conjugate, #2846) |
IC, IF, IH, WB |
Human, Monkey, Mouse, Rat, Drosophila |
immunogen = synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1 |
detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46; may cross-react with 4E-BP2 and 4E-BP3 when phosphor. at equivalent sites |
- |
|
|
Cell Signaling |
polyclonal
|
Rabbit
(liquid, protein-A and affinity purified, HEPES, saline, BSA, glycerol) |
IP, WB |
Human, Monkey, Mouse, Rat |
immunogen = synthetic peptide (KLH-coupled) corresponding to residues surrounding Ser112 of human 4E-BP1 |
detects endogenous levels of total 4E-BP1, independent of phosphorylation |
- |
|
|
Cell Signaling |
polyclonal
|
Rabbit
(liquid, affinity purified, sodium HEPES, BSA, glycerol) |
WB |
Human, Monkey, Mouse, Rat |
immunogen = synthetic peptide (KLH-coupled) derived from the carboxy terminal sequence of eIF2α. |
detects endogenous levels of of total eIF2α protein |
- |
|
|
Cell Signaling |
monoclonal
iGg |
Rabbit
(liquid, affinity purified, sodium HEPES, BSA, glycerol) |
IH, IP, WB |
Human, Monkey, Mouse, Rat, Drosophila |
immunogen = synthetic peptide (KLH-coupled) derived from the carboxy terminal sequence of eIF2α. |
detects endogenous eIF2alpha only when phosphorylated at Ser51; does not recognize eIF2alpha phosphorylated at other sites |
- |
|
|
Cell Signaling |
polyclonal
|
Rabbit
(liquid, affinity purified, sodium HEPES, BSA, glycerol) |
WB |
Human, Monkey, Mouse, Rat |
immunogen = synthetic phospho peptide (KLH coupled) corresp. to residues surrounding Ser209 of eIF4E |
detects endogenous levels of eIF4E only when phosphorylated at serine 209 |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, gelatin, azide) |
IF, IH, IP, WB |
Human |
immunogen = raised against a recombinant protein corresp. to the N-terminal 118 aa of human 4E-BP1 |
4E-BP1 |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
Pig, Human, Mouse, Rat |
immunogen = raised against full length human 4E-BP1 |
4E-BP1 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, gelatin, azide, affinity purified) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = mapping at the N-terminus of human 4E-BP1 |
4E-BP1 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, gelatin, azide, affinity purified) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = mapping at the C-terminus of mouse 4E-BP1 |
4E-BP1 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = epitope corresp. to aa1-117 representing full length rat 4E-BP1 |
detection of translational repressor 4E-BP1 and, to a lesser extent, PHAS-2 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = epitope corresp. to phosphorylated Thr 36 of human 4E-BP1 |
4E-BP1 phosphorylated at Thr36 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = epitope corresp. to aa1-117 representing full length rat 4E-BP1 |
4E-BP1 phosphorylated at Ser65 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = epitope corresp.to a short aa seq. containing phosphorylated Ser 65 and Thr 70 of human 4E-BP1 |
4E-BP1 phosphorylated at Ser65 and Thr70 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = epitope corresp. to phosphorylated Thr 70 of human 4E-BP1 |
4E-BP1 phosphorylated at Thr70 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = epitope corresp. to phosphorylated Thr69 of human 4E-BP1 |
4E-BP1 phosphorylated at Thr69 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = epitope corresp. to aa29-68 mapping within an internal region of human 4E-BP1 |
detects 4E-BP1, 4E-BP2 and /or 4E-BP3 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
IF, IH, IP, WB |
Human, Mouse, Rat |
immunogen = epitope corresp. to phosphorylated Thr 36 of human 4E-BP1/2/3 |
detects 4E-BP1, 4E-BP2 and /or 4E-BP3 phosphorylated at Thr 36 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
ELISA, IF, WB |
Human, Mouse, Rat |
immunogen = epitope corresp. to phosphorylated Thr 45 of human 4E-BP1/2/3 |
detection of Thr 45 phosphorylated 4E-BP1/2/3 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
IF, IH, IP, WB |
Human, Mouse, Rat |
immunogen = epitope corresp. to phosphorylated Thr 45of human 4E-BP1/2/3 |
detection of Thr 45 phosphorylated 4E-BP1/2/3 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = epitope corresp. to phosphorylated Thr 45 of human 4E-BP1 |
detection of Thr 45 phosphorylated 4E-BP1 and correspondingly phosphorylated 4E-BP2 and 4E-BP3 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, Tris-citrate/phosphate, azide, affinity purified) |
EMSA, WB |
Pig, Human, Mouse, Rat |
immunogen = synthetic peptide (human) - which represents a portion of human eIF-4EBP1 encoded in part by exons 2and 3 |
detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1 |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, protein A purified) |
ELISA, IH, WB |
Human |
immunogen = Ni-NTA purified truncated recombinant eIF4EBP1 expressed in E. coli strain BL21 (DE3) |
specific for elF4EBP1 |
- |
|
|
Abcam |
monoclonal
IgG |
Rabbit
(liquid, Tris-glycine saline, BSA, azide, glycerol) |
FC, IC, IH, IP, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to residues near the N-term of human eIF4EBP1 (aa16-33): P A T R R V V L G D G V Q L P P G D C |
eIF4EBP1 |
- |
|
|
Abcam |
monoclonal
IgG |
Rabbit
(liquid, Tris-glycine saline, BSA, azide, glycerol) |
FC, IH, IP, WB |
Human |
immunogen = synthetic peptide corresp. to residues near the C-term of human 4E-BP1 |
recognises 4E-BP1 (eIF4E-binding protein) also known as PHAS |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
immunogen = Synthetic phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of serine 64 (R-N-Sp-P-V) |
detects endogenous levels of eIF4EBP1 only when phosphorylated at serine 64 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
immunogen = synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-Tp-P-G) |
detects endogenous levels of eIF4EBP1 only when phosphorylated at threonine 36; may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
immunogen = synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-Tp-P-G) |
detects endogenous levels of eIF4EBP1 only when phosphorylated at threonine 45; may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
immunogen = synthetic non phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of threonine 36 (S-T-Tp-P-G) |
recognises endogenous levels of total eIF4EBP1 protein; may cross-react with 4E-BP2 and 4E-BP3 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
immunogen = synthetic non phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of threonine 45 (S-T-Tp-P-G) |
recognises endogenous levels of total eIF4EBP1 protein; may cross-react with 4E-BP2 and 4E-BP3 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
immunogen = synthetic non-phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of Threonine 70 (A K T P P) |
detects endogenous levels of total eIF4EBP1 protein |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
immunogen = synthetic non-phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of serine 65 (R-N-Sp-P-V) |
detects endogenous levels of total eIF4EBP1 protein |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
IC, IF, WB |
Human, Mouse |
immunogen = 14 aa synthetic peptide from near the C-terminus of human eIF4EBP1 |
eIF4EBP1 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified) |
ELISA, IH |
Human, Mouse, Rat |
immunogen = synthetic phosphopeptide derived from human eIF4EBP1 around the phosphorylation site threonine 70 (A-K-Tp-P-P) |
detects endogenous levels of eIF4EBP1 only when phosphorylated at threonine 69 |
- |
|
|
Abcam |
monoclonal
IgG |
Rabbit
(liquid, BSA, supernatant, tris-glycine saline, glycerol, azide) |
IH, WB |
Human |
immunogen = a phospho specific peptide corresp. to residues surrounding threonine 70 of human eIF4EBP1 |
detects eIF4EBP1 phosphorylated at threonine 70 |
- |
|
|
Abcam |
monoclonal
IgG |
Rabbit
(liquid, BSA, supernatant, tris-glycine saline, glycerol, azide) |
WB |
Human, Mouse |
immunogen = a phospho-peptide corresponding to residues surrounding threonine 37 of eIF4EBP1 |
detects eIF4EBP1 phosphorylated at threonine 37 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, glycerol, azide, affinity purified) |
WB |
Human, Mouse |
immunogen = synthetic phosphopeptide derived from the region of human eIF4EBP1 that contains threonine 46; seq. is conserved in human, mouseand rat |
recognises eIF4EBP1 that is phosphorylated at threonine 46 |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, BSA, azide, protein G purified) |
WB |
Human, Mouse, Rat |
immunogen = recombinant human eIF4EBP1 protein expressed in E. coli |
specific for elF4EBP1 |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
- |
detects endogenous levels of 4E-BP1 protein |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
- |
detects endogenous levels of 4E-BP1 protein |
- |
|
|
Novus Biologicals |
monoclonal
IgG |
Rabbit
(liquid, ascites, tris-glycine, NaCl, BSA, glycerol, azide) |
FC, IH, IP, WB |
Human |
immunogen = synthetic peptide corresp. to residues near the C-term of human4E-BP1 |
4E-BP1 |
- |
|
|
Novus Biologicals |
monoclonal
IgG |
Rabbit
(liquid, ascites, tris-glycine, NaCl, BSA, glycerol, azide) |
FC, IC, IH, IP, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to residues near the N-term of human eIF4EBP1 (aa16-33): P A T R R V V L G D G V Q L P P G D C |
4E-BP1 |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, antiserum, PBS, azide) |
WB |
Human, Mouse |
immunogen = human 4E-BP1 / initiation factor 4E binding protein 1 peptide |
4E-BP1 |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, antiserum, PBS, azide, glycerol, affinity purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthesized non-phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-Tp-P-G) |
detects endogenous levels of total 4E-BP1 protein; may cross-react with 4E-BP2 and 4E-BP3 |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, antiserum, PBS, azide, glycerol, affinity purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthesized non-phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-Tp-P-G) |
detects endogenous levels of total 4E-BP1 protein; may cross-react with 4E-BP2 and 4E-BP3 |
- |
|
|
Novus Biologicals |
monoclonal
IgG |
Mouse
(liquid, ascites, azide) |
ELISA, WB |
Human |
immunogen = Ni-NTA purified truncated recombinant 4EBP1 expressed in E. coli strain BL21 (DE3) |
4E-BP1 |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
- |
detects endogenous levels of 4E-BP1 phosphorylated at Ser64 |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
ELISA, IH |
Human, Mouse, Rat |
- |
detects endogenous levels of 4E-BP1 phosphorylated at Thr69 |
- |
|
|
Novus Biologicals |
monoclonal
IgG |
Rabbit
(liquid, ascites, tris-glycine, NaCl, BSA, glycerol, azide) |
WB |
Human, Mouse |
immunogen = phospho-specific peptide corresp. to residues surrounding serine 65 of human 4E-BP1 |
detects 4E-BP1 phosphorylated at serine 65 |
- |
|
|
Novus Biologicals |
monoclonal
IgG |
Rabbit
(liquid, ascites, azide) |
IH, WB |
Human |
immunogen = phospho-specific peptide corresp. to residues surrounding threonine 70 of human 4E-BP1 |
detects 4E-BP1 phosphorylated at threonine 70 |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, PBS, NaCl, azide, glycerol, affinity purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-Tp-P-G) |
detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 36; may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, PBS, NaCl, azide, glycerol, affinity purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-Tp-P-G) |
detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 45; may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites |
- |
|
|
EMD Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, HEPES, BSA, NaCl, glycerol, no preservative) |
IB, WB |
Zebra fish, Human, Monkey, Mouse, Rat, Drosophila |
immunogen = synthetic phosphopeptide corresp. to aa surrounding the Thr37 and Thr46 phosphorylation sites of mouse 4EBP1, coupled to KLH |
recognizes the ~20 kDa 4EBP1 protein, phosphorylated at Thr37 and Thr46 |
- |
|
|
EMD Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, HEPES, BSA, NaCl, glycerol, no preservative) |
IB, IP, WB |
Human, Mouse, Rat, Drosophila |
immunogen = synthetic phosphopeptide corresp. to aa surrounding the Thr70 phosphorylation sites of rat 4EBP1 |
recognizes the ~20 kDa 4EBP1 protein, phosphorylated at Thr70 |
- |
|
|
EMD Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, BBS, EDTA, NaCl, no preservative) |
EMSA, IB, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide (S P E D K R A G G E E S Q F E M D I) corresp. to aa101-118 of human PHAS-I, conjugated to KLH |
recognizes the ~19-25 kDa multiple phosphorylation states of native and recombinant PHAS-I protein |
- |
|
|
AnaSpec |
polyclonal
IgG |
Rabbit
(liquid, purified, PBS, azide) |
ELISA, IC, WB |
Human, Mouse |
immunogen = 14 amino acid peptide from near the carboxy-terminus of human 4E-BP1 |
dertects 4E-BP1 |
- |
|
|
Upstate and Chemicon are Millipore |
monoclonal
IgG1,κ |
Mouse
(liquid, purified, Tris-glycine, saline, azide) |
IP, WB |
Mammal (various), S. cerevisiae |
immunogen = Crude calmodulin-binding proteins from Trypanosoma brucei isolated using calmodulin-affinity chromatography |
recognizes EF-1α (Elongation factor-1 alpha), Mr 53 kDa |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, affinity purified, PBS, azide, gelatin) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = apeptide mapping at the N-terminus of eIF3η of human origin |
detects eIF3η |
- |
|
|
None |
polyclonal
IgG |
Rabbit
(affinity purified, PBS, glycerol, azide) |
WB |
Human |
immunogen = recombinant (partial), N-terminal |
recognizes human eIF-4E |
discontinued |