eIF4A
Cell Signaling
polyclonal
Rabbit
(liquid, affinity purified)
-
Human, Monkey, Mouse, Rat
immunogen = synthetic peptide (KLH-coupled) derived from residues surrounding Phe52 of human eIF4A
detects endogenous levels of total eIF4A1, eIF4A2 and eIF4A3 proteins
-
eIF4A
Santa Cruz
polyclonal
IgG
Goat
(liquid, affinity purified, PBS, azide, gelatin)
-
S. cerevisiae
immunogen = peptide mapping near the N-terminus of eIF4A of Saccharomyces cerevisiaeorigin
detects eIF4A
-
eIF4AI/II
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = amino acids186-330 mapping within an internal region of eIF4AI of human origin
detects eIF4AI and eIF4AII
-
eIF4AI
Santa Cruz
polyclonal
IgG
Goat
(liquid, affinity purified, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = peptide mapping at the N-terminus of eIF4AI of human origin
detects eIF4AI
-
eIF4AII
Santa Cruz
polyclonal
IgG
Goat
(liquid, affinity purified, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = peptide mapping at the N-terminus of eIF4AII of human origin
detects 46 kDa eIF4AII
-
eIF4AIII (3F1)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
-
Human
immunogen = recombinantGST-tagged eIF4AIII protein of human origin
detects eIF4AIII
-
eIF4E
Cell Signaling
polyclonal
Rabbit
(liquid, affinity purified)
-
Zebra fish, Human, Monkey, Mouse, Rat
immunogen = peptide (KLH coupled) corresp. to residues surrounding Ser209 of eIF4E
detects endogenous levels of total eIF4E
-
eIF-4E
None
polyclonal
IgG
Rabbit
(affinity purified, PBS, glycerol, azide)
-
Human
immunogen = recombinant (partial), N-terminal
recognizes human eIF-4E
discontinued
4E-BP1 phos Thr37/46
Cell Signaling
polyclonal
Rabbit
(liquid, protein-A and affinity purified, HEPES, saline, BSA, glycerol)
-
Human, Monkey, Mouse, Rat
immunogen = synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1
detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46; may cross-react with 4E-BP2 and 4E-BP3 when phosphor. at equivalent sites
-
4E-BP1 phos Thr37/46 (236B4)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, protein-A and affinity purified, HEPES, saline, BSA, glycerol, azideAvailable as Alexa Fluor® 488 Conjugate, #2846)
-
Human, Monkey, Mouse, Rat, Drosophila
immunogen = synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1
detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46; may cross-react with 4E-BP2 and 4E-BP3 when phosphor. at equivalent sites
-
4E-BP1 total
Cell Signaling
polyclonal
Rabbit
(liquid, protein-A and affinity purified, HEPES, saline, BSA, glycerol)
-
Human, Monkey, Mouse, Rat
immunogen = synthetic peptide (KLH-coupled) corresponding to residues surrounding Ser112 of human 4E-BP1
detects endogenous levels of total 4E-BP1, independent of phosphorylation
-
eIF2α
Cell Signaling
polyclonal
Rabbit
(liquid, affinity purified, sodium HEPES, BSA, glycerol)
-
Human, Monkey, Mouse, Rat
immunogen = synthetic peptide (KLH-coupled) derived from the carboxy terminal sequence of eIF2α.
detects endogenous levels of of total eIF2α protein
-
eIF2α phos (119A11)
Cell Signaling
monoclonal
iGg
Rabbit
(liquid, affinity purified, sodium HEPES, BSA, glycerol)
-
Human, Monkey, Mouse, Rat, Drosophila
immunogen = synthetic peptide (KLH-coupled) derived from the carboxy terminal sequence of eIF2α.
detects endogenous eIF2alpha only when phosphorylated at Ser51; does not recognize eIF2alpha phosphorylated at other sites
-
eIF4E phos Ser209
Cell Signaling
polyclonal
Rabbit
(liquid, affinity purified, sodium HEPES, BSA, glycerol)
-
Human, Monkey, Mouse, Rat
immunogen = synthetic phospho peptide (KLH coupled) corresp. to residues surrounding Ser209 of eIF4E
detects endogenous levels of eIF4E only when phosphorylated at serine 209
-
4E-BP1 (11G12C11)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Human
immunogen = raised against a recombinant protein corresp. to the N-terminal 118 aa of human 4E-BP1
4E-BP1
-
4E-BP1 (P-1)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Pig, Human, Mouse, Rat
immunogen = raised against full length human 4E-BP1
4E-BP1
-
4E-BP1 (N-19)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = mapping at the N-terminus of human 4E-BP1
4E-BP1
-
4E-BP1 (C19)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = mapping at the C-terminus of mouse 4E-BP1
4E-BP1
-
4E-BP1 (R-113)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
immunogen = epitope corresp. to aa1-117 representing full length rat 4E-BP1
detection of translational repressor 4E-BP1 and, to a lesser extent, PHAS-2
-
4E-BP1 (phos Thr36)-R
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
immunogen = epitope corresp. to phosphorylated Thr 36 of human 4E-BP1
4E-BP1 phosphorylated at Thr36
-
4E-BP1 (phos Ser65)-R
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
immunogen = epitope corresp. to aa1-117 representing full length rat 4E-BP1
4E-BP1 phosphorylated at Ser65
-
4E-BP1 phos Ser65/Thr70 R
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
immunogen = epitope corresp.to a short aa seq. containing phosphorylated Ser 65 and Thr 70 of human 4E-BP1
4E-BP1 phosphorylated at Ser65 and Thr70
-
4E-BP1 (phos Thr70)-R
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
immunogen = epitope corresp. to phosphorylated Thr 70 of human 4E-BP1
4E-BP1 phosphorylated at Thr70
-
4E-BP1 (phos Thr70)-R
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
immunogen = epitope corresp. to phosphorylated Thr69 of human 4E-BP1
4E-BP1 phosphorylated at Thr69
-
4E-BP1/2/3 (H-40)-R
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
immunogen = epitope corresp. to aa29-68 mapping within an internal region of human 4E-BP1
detects 4E-BP1, 4E-BP2 and /or 4E-BP3
-
4E-BP1/2/3 (phos Thr36)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
immunogen = epitope corresp. to phosphorylated Thr 36 of human 4E-BP1/2/3
detects 4E-BP1, 4E-BP2 and /or 4E-BP3 phosphorylated at Thr 36
-
4E-BP1/2/3 (phos Thr45)-R
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
immunogen = epitope corresp. to phosphorylated Thr 45 of human 4E-BP1/2/3
detection of Thr 45 phosphorylated 4E-BP1/2/3
-
4E-BP1/2/3 (phos Thr45)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
immunogen = epitope corresp. to phosphorylated Thr 45of human 4E-BP1/2/3
detection of Thr 45 phosphorylated 4E-BP1/2/3
-
4E-BP1/2/3 (phos Thr45)-R
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
immunogen = epitope corresp. to phosphorylated Thr 45 of human 4E-BP1
detection of Thr 45 phosphorylated 4E-BP1 and correspondingly phosphorylated 4E-BP2 and 4E-BP3
-
elF4EBP1
Abcam
polyclonal
IgG
Rabbit
(liquid, Tris-citrate/phosphate, azide, affinity purified)
-
Pig, Human, Mouse, Rat
immunogen = synthetic peptide (human) - which represents a portion of human eIF-4EBP1 encoded in part by exons 2and 3
detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1
-
elF4EBP1 (11G12C11)
Abcam
monoclonal
IgG1
Mouse
(liquid, PBS, azide, protein A purified)
-
Human
immunogen = Ni-NTA purified truncated recombinant eIF4EBP1 expressed in E. coli strain BL21 (DE3)
specific for elF4EBP1
-
elF4EBP1 (Y329)
Abcam
monoclonal
IgG
Rabbit
(liquid, Tris-glycine saline, BSA, azide, glycerol)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to residues near the N-term of human eIF4EBP1 (aa16-33): P A T R R V V L G D G V Q L P P G D C
eIF4EBP1
-
elF4EBP1 (Y330)
Abcam
monoclonal
IgG
Rabbit
(liquid, Tris-glycine saline, BSA, azide, glycerol)
-
Human
immunogen = synthetic peptide corresp. to residues near the C-term of human 4E-BP1
recognises 4E-BP1 (eIF4E-binding protein) also known as PHAS
-
elF4EBP1 phos Ser64
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = Synthetic phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of serine 64 (R-N-Sp-P-V)
detects endogenous levels of eIF4EBP1 only when phosphorylated at serine 64
-
elF4EBP1 phos Thr36
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-Tp-P-G)
detects endogenous levels of eIF4EBP1 only when phosphorylated at threonine 36; may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites
-
elF4EBP1 phos Thr45
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-Tp-P-G)
detects endogenous levels of eIF4EBP1 only when phosphorylated at threonine 45; may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites
-
elF4EBP1
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic non phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of threonine 36 (S-T-Tp-P-G)
recognises endogenous levels of total eIF4EBP1 protein; may cross-react with 4E-BP2 and 4E-BP3
-
elF4EBP1
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic non phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of threonine 45 (S-T-Tp-P-G)
recognises endogenous levels of total eIF4EBP1 protein; may cross-react with 4E-BP2 and 4E-BP3
-
elF4EBP1
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic non-phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of Threonine 70 (A K T P P)
detects endogenous levels of total eIF4EBP1 protein
-
elF4EBP1
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic non-phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of serine 65 (R-N-Sp-P-V)
detects endogenous levels of total eIF4EBP1 protein
-
elF4EBP1
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human, Mouse
immunogen = 14 aa synthetic peptide from near the C-terminus of human eIF4EBP1
eIF4EBP1
-
elF4EBP1 phos Thr70
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic phosphopeptide derived from human eIF4EBP1 around the phosphorylation site threonine 70 (A-K-Tp-P-P)
detects endogenous levels of eIF4EBP1 only when phosphorylated at threonine 69
-
elF4EBP1 phos Thr70 (EPR654(2)AY)
Abcam
monoclonal
IgG
Rabbit
(liquid, BSA, supernatant, tris-glycine saline, glycerol, azide)
-
Human
immunogen = a phospho specific peptide corresp. to residues surrounding threonine 70 of human eIF4EBP1
detects eIF4EBP1 phosphorylated at threonine 70
-
elF4EBP1 phos Thr37 (EPR729(2)Y)
Abcam
monoclonal
IgG
Rabbit
(liquid, BSA, supernatant, tris-glycine saline, glycerol, azide)
-
Human, Mouse
immunogen = a phospho-peptide corresponding to residues surrounding threonine 37 of eIF4EBP1
detects eIF4EBP1 phosphorylated at threonine 37
-
elF4EBP1 phos Thr46
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, glycerol, azide, affinity purified)
-
Human, Mouse
immunogen = synthetic phosphopeptide derived from the region of human eIF4EBP1 that contains threonine 46; seq. is conserved in human, mouseand rat
recognises eIF4EBP1 that is phosphorylated at threonine 46
-
elF4EBP1 (554R16)
Abcam
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, BSA, azide, protein G purified)
-
Human, Mouse, Rat
immunogen = recombinant human eIF4EBP1 protein expressed in E. coli
specific for elF4EBP1
-
4E-BP1
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
-
detects endogenous levels of 4E-BP1 protein
-
4E-BP1
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
-
detects endogenous levels of 4E-BP1 protein
-
4E-BP1 (Y330)
Novus Biologicals
monoclonal
IgG
Rabbit
(liquid, ascites, tris-glycine, NaCl, BSA, glycerol, azide)
-
Human
immunogen = synthetic peptide corresp. to residues near the C-term of human4E-BP1
4E-BP1
-
4E-BP1 (Y329)
Novus Biologicals
monoclonal
IgG
Rabbit
(liquid, ascites, tris-glycine, NaCl, BSA, glycerol, azide)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to residues near the N-term of human eIF4EBP1 (aa16-33): P A T R R V V L G D G V Q L P P G D C
4E-BP1
-
4E-BP1
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, antiserum, PBS, azide)
-
Human, Mouse
immunogen = human 4E-BP1 / initiation factor 4E binding protein 1 peptide
4E-BP1
-
4E-BP1
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, antiserum, PBS, azide, glycerol, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized non-phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-Tp-P-G)
detects endogenous levels of total 4E-BP1 protein; may cross-react with 4E-BP2 and 4E-BP3
-
4E-BP1
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, antiserum, PBS, azide, glycerol, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized non-phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-Tp-P-G)
detects endogenous levels of total 4E-BP1 protein; may cross-react with 4E-BP2 and 4E-BP3
-
4E-BP1 (9E12D9)
Novus Biologicals
monoclonal
IgG
Mouse
(liquid, ascites, azide)
-
Human
immunogen = Ni-NTA purified truncated recombinant 4EBP1 expressed in E. coli strain BL21 (DE3)
4E-BP1
-
4E-BP1 phos Ser64
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human, Mouse, Rat
-
detects endogenous levels of 4E-BP1 phosphorylated at Ser64
-
4E-BP1 phos Thr69
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human, Mouse, Rat
-
detects endogenous levels of 4E-BP1 phosphorylated at Thr69
-
4E-BP1 phos Ser65 (EP353(2)Y)
Novus Biologicals
monoclonal
IgG
Rabbit
(liquid, ascites, tris-glycine, NaCl, BSA, glycerol, azide)
-
Human, Mouse
immunogen = phospho-specific peptide corresp. to residues surrounding serine 65 of human 4E-BP1
detects 4E-BP1 phosphorylated at serine 65
-
4E-BP1 phos Thr70 (EPR654(2)A)
Novus Biologicals
monoclonal
IgG
Rabbit
(liquid, ascites, azide)
-
Human
immunogen = phospho-specific peptide corresp. to residues surrounding threonine 70 of human 4E-BP1
detects 4E-BP1 phosphorylated at threonine 70
-
4E-BP1 phos Thr36
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, azide, glycerol, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-Tp-P-G)
detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 36; may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites
-
4E-BP1 phos Thr45
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, azide, glycerol, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-Tp-P-G)
detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 45; may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites
-
elF4EBP1 phos Thr37/46
EMD Biosciences, Inc.
polyclonal
IgG
Rabbit
(liquid, HEPES, BSA, NaCl, glycerol, no preservative)
-
Zebra fish, Human, Monkey, Mouse, Rat, Drosophila
immunogen = synthetic phosphopeptide corresp. to aa surrounding the Thr37 and Thr46 phosphorylation sites of mouse 4EBP1, coupled to KLH
recognizes the ~20 kDa 4EBP1 protein, phosphorylated at Thr37 and Thr46
-
elF4EBP1 phos Thr70
EMD Biosciences, Inc.
polyclonal
IgG
Rabbit
(liquid, HEPES, BSA, NaCl, glycerol, no preservative)
-
Human, Mouse, Rat, Drosophila
immunogen = synthetic phosphopeptide corresp. to aa surrounding the Thr70 phosphorylation sites of rat 4EBP1
recognizes the ~20 kDa 4EBP1 protein, phosphorylated at Thr70
-
elF4EBP1 binding protein
EMD Biosciences, Inc.
polyclonal
IgG
Rabbit
(liquid, BBS, EDTA, NaCl, no preservative)
-
Human, Mouse, Rat
immunogen = synthetic peptide (S P E D K R A G G E E S Q F E M D I) corresp. to aa101-118 of human PHAS-I, conjugated to KLH
recognizes the ~19-25 kDa multiple phosphorylation states of native and recombinant PHAS-I protein
-
4E-BP1
Anaspec
polyclonal
IgG
Rabbit
(liquid, purified, PBS, azide)
-
Human, Mouse
immunogen = 14 amino acid peptide from near the carboxy-terminus of human 4E-BP1
dertects 4E-BP1
-
EF1α (CBP-KK1)
Upstate and Chemicon are Millipore
monoclonal
IgG1 ,κ
Mouse
(liquid, purified, Tris-glycine, saline, azide)
-
Mammal (various), S. cerevisiae
immunogen = Crude calmodulin-binding proteins from Trypanosoma brucei isolated using calmodulin-affinity chromatography
recognizes EF-1α (Elongation factor-1 alpha), Mr 53 kDa
-
eIF3η (N-20)
Santa Cruz
polyclonal
IgG
Goat
(liquid, affinity purified, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = apeptide mapping at the N-terminus of eIF3η of human origin
detects eIF3η
-