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Antibodies

298 Results

Name Source Clonality &
Isotype
Host &
Formulation
Method Reactivity Immunogen/
Epitope
Specificity Availability
Proteases From manufacturers (100)
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide based on the C- terminal end of the zinc finger domain of human Calpain 15 Calpain 15; no cross reaction with the other calpain family members -
Abcam polyclonal
IgG1
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide based on the N- terminal end of the large subunit (Domain I) of human Calpain 2 reacts with the propeptide domain, (Domain I) of Calpain 2, N-terminal from the activation cleavage site. It binds to reduced and non reduced Calpain 2, but does not recognize N-processed Calpain 2. Does not recognise the other calpain family members (calpain 1, calpain 94, ncl 2, ncl 3, etc.). -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide based on the C- terminal end of the large subunit (Domain III) of human Calpain 2 reacts with Domain III of Calpain 2, and detects both latent and N-processed (activated) Calpain 2. Does not recognise the other calpain family members (calpain 1, calpain 94, ncl 2, ncl 3, etc.). -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human Immunogen = synthetic peptide based on the calcium binding region of Domain III, in the large subunit of Human Calpain 2 reacts with Domain III of Calpain 2, and detects both latent and N-processed (activated) Calpain 2, binds to both reduced and non reduced forms. Does not recognise the other calpain family members (calpain 1, calpain 94, ncl 2, ncl 3, etc.). -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human Immunogen = synthetic peptide based on domain I of human Calpain 3 large subunit binds to Calpain 3 (reduced and non reduced), and recognizes both the latent and active forms of the protein. Does not recognise the other calpain family members (calpain 1, calpain 94, ncl 2, ncl 3, etc.). -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Rat Immunogen = synthetic peptide corresp. to the N-terminal end of the domain I from rat calpain 3 (large subunit) binds to the Domain I, large subinit of calpain 3, LP82, LP85, LP88 and LP90 calpain. Does not recognise the other calpain family members (calpain 1, calpain 94, ncl 2, ncl 3, etc.). -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human Immunogen = synthetic peptide based on the catalytic domain (domain-II) of human Calpain 3 large subunit Calpain 3. Does not recognise the other calpain family members (calpain 1, calpain 94, ncl 2, ncl 3, etc.). -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human Immunogen = synthetic peptide based on Insert 1, in domain-II of human Calpain 3 large subunit Calpain 3. Does not recognise the other calpain family members (calpain 1, calpain 94, ncl 2, ncl 3, etc.). May crossreact with rat 88 and 90 kD novel Calpains due to Domain II sequence similarities. -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human Immunogen = synthetic peptide based on catalytic domain (domain-II) of human Calpain 3 large subunit recognizes latent and active Calpain 3, recognizes all of the Calpain 3 isoforms; Does not cross react with the other calpain family members (calpain 1, calpain 94, ncl 2, ncl 3, etc.). -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human Immunogen = synthetic peptide based on domain III of human Calpain 3 large subunit Calpain 3, may cross react with rat 88 and 90kDa Calpain 3 forms.No cross reaction with the other calpain family members (calpain 1, calpain 94, ncl 2, ncl 3, etc.). -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human Immunogen = synthetic peptide of human Calpain 3 based on Insert 2, in domain-III of Calpain 3 large subunit recognises Domain III, (Insert 2), of Calpain 3 large subunit. No cross reaction with the other calpain family members (calpain 1, calpain 94, ncl 2, ncl 3, etc.). -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Mouse, Rat Immunogen = synthetic peptide based on a unique insert found in the EF hand (the calcium binding domain IV of LP85 calpain large subunit) of Rat Calpain 3 binds to Calpain 3, and recognizes both the latent and active forms; no cross reaction with the other calpain family members (calpain 1, calpain 94, ncl 2, ncl 3, etc.). -
Abcam monoclonal
IgG2a
Mouse
(liquid, supernatant, azide)
WB Rabbit, Chicken, Pig, Human, Mouse, Rat Immunogen = synthetic peptide: R L R N P W G Q V E W N G S W S, corresp. to aa355-370 of human Calpain 3 reacts with calpain 3 (94kD) plus pre-and post-autolysed forms of the ubiquitous calpains 1 and 2 (µ and m-calpains) -
Abcam monoclonal
IgG2b
Mouse
(liquid, supernatant, azide)
WB Dog, Rabbit, Sheep, Human, Hamster Immunogen = synthetic peptide: M P T V I S A S V A P R T A A E P R S, corresp. to aa1-19 of human Calpain 3 reacts with calpain 3 (94kD) plus pre-and post-autolysed forms of the ubiquitous calpains 1 and 2 (µ and m-calpains) -
Abcam monoclonal
IgG1
Mouse
(liquid, PBS, ascites, azide)
WB Cow, Human Immunogen = purified bovine skeletal muscle 28 kDa Calpain subunit Calpain 28 kDa subunit; no cross-reaction with the human 80 kDa m-Calpain subunit -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide based on the N- terminal end of the large subunit of human Calpain 5 reacts to the first 30 amino acids of the "propeptide domain," (Domain-I) of Calpain 5, N-terminal from the activation cleavage site. no cross reaction with the other calpain family members (Calpain 1, Calpain 2, Calpain 3, ncl 3, etc.). -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide based on the C- terminal end of the large subunit of human Calpain 5 binds to reduced and non-reduced Calpain 5, reacts to Domain-IV of Calpain 5, and detects both latent and N-processed (activated) Calpain 5.No cross reaction with the other calpain family members (Calpain 1, Calpain 2, Calpain 3, ncl 3, etc.). -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide based on Domain II in the large subunit of human Calpain 5 binds to Calpain 5, recognizing both the reduced and non reduced protein. No cross reaction with the other calpain family members (Calpain 1, Calpain 2, Calpain 3, ncl 3, etc.). -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide corresp. to the C-terminal end of the large subunit of human Calpain binds to Calpain 6, reacts to Domain-T of Calpain 6, and detects both latent and N-processed (activated) Calpain 6. No cross reaction with the other calpain family members (Calpain 1, Calpain 2, Calpain 3, ncl 3, etc.). -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide corresp. to the domain-I of the large subunit of human Calpain binds to Domain-l, large subunit of Calpain 6 but does not cross react with the other calpain family members (Calpain 1, Calpain 2, Calpain 3, ncl 3, etc.) -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide corresp. to the domain-II of the large subunit of human Calpain binds to Domain-II, large subunit of Calpain 6 but does not cross react with the other calpain family members (Calpain 1, Calpain 2, Calpain 3, ncl 3, etc.) -
Abcam polyclonal
IgG
Rabbit
(liquid, azide, glycerol, affinity purified)
WB Human, Mouse, Rat Immunogen = Synthetic peptide based on C-terminal Domain I of the large subunit of human calpain 7 Calpain 7. No cross reaction with the other calpain family members (calpain 1, calpain 2, Calpain 3, ncl 3, etc). -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human, Mouse, Rat Immunogen = Synthetic peptide based on N-terminal Domain I of the large subunit of human calpain 7 N-terminal end of Domain I of Calpain 7. No cross reaction with the other calpain family members (calpain 1, calpain 2, Calpain 3, ncl 3, etc). -
Abcam polyclonal
IgG
Rabbit
(liquid, azide, glycerol, affinity purified)
WB Human, Mouse, Rat Immunogen = Synthetic peptide based on Domain III of the large subunit of human calpain 7 reacts to the carboxy end of Domain III of Calpain 7. No cross reaction with the other calpain family members (calpain 1, calpain 2, Calpain 3, ncl 3, etc). -
Abcam polyclonal
IgG
Rabbit
(liquid, azide, PBS, glycerol, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide corresp. to the Domain N of the large subunit of human Calpain 7 reacts to the C-terminal end of Domain-N of Calpain 7 and recognizes latent and N-processed Calpain 7. No cross reaction with the other calpain family members (calpain 1, calpain 2, Calpain 3, ncl 3, etc). -
Abcam polyclonal
IgG
Rabbit
(liquid, azide, glycerol, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide (rat), based on the domain III of the large Calpain 8 subunit full length Calpain 8. No cross reaction with the other calpain family members (calpain 1, calpain 2, Calpain 3, ncl 3, etc). -
Abcam polyclonal
IgG
Rabbit
(liquid, azide, glycerol, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide (rat), based on the N-terminal end of the large subunit of Calpain 8 Calpain 8. No cross reaction with the other calpain family members (calpain 1, calpain 2, Calpain 3, ncl 3, etc). -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human Immunogen = synthetic peptide EF hand region (human) Calpain 9 -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide (human) based on Calpain 9 domain I in the large subunit binds to both reduced and non reduced Calpain 9 proteins. It recognizes latent but not N-truncated (active) Calpain 9. No cross reaction with the other calpain family members (m calpain, calpain 94, ncl 2, ncl 3, etc.) -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide (human) based on Calpain 9 Domain II (catalytic domain) in the large subunit binds to both reduced and non-reduced Calpain 9 proteins. It recognizes latent and N-truncated (active) Calpain 9. No cross reaction with the other calpain family members (m calpain, calpain 94, ncl 2, ncl 3, etc.) -
Abcam polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide (human) based on domain-IV in the large subunit recognizes latent and N-truncated (active) calpain 9, but not the truncated form lacking the domain-IV. No cross reaction with the other calpain family members (m calpain, calpain 94, ncl 2, ncl 3, etc.) -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS azide, protein G purified)
ELISA, WB Human, Rat Immunogen = synthetic peptide (10-30 aa in length) in the region of aa152-169 of human Calpain 5, KLH conjugated Calpain 5 (HtrA3) -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS azide, protein G purified)
ELISA, WB Human Immunogen = synthetic peptide within region aa400-500 of human Calpain 5 Calpain 5 (HtrA3) -
Abcam polyclonal
IgG
Rabbit
(liquid, azide, PBS, glycerol, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide located within aa250-300 of human Calpain 5(HtrA3) recognises both Isoform 1 (heart and skeletal muscle) and Isoform 2 (placenta and kidney) Calpain 5 -
Abcam polyclonal
IgG
Rabbit
(liquid, azide, NaCl, PBS, glycerol, affinity purified)
WB Human Immunogen = synthetic peptide based on the Kazal domain of human Calpain 5 (HtrA3) recognises both Isoform 1 (heart and skeletal muscle) and Isoform 2 (placenta and kidney) Calpain 5 (HtrA3) -
Abcam polyclonal
IgG
Rabbit
(liquid, azide, NaCl, PBS, glycerol, affinity purified)
WB Human Immunogen = synthetic peptide based on the PDZ domain of human Calpain 5 (HtrA3) recognizes the longer forms of HtrA3 (Calpain 5), and the shorter form more weakly -
Abcam polyclonal
IgG
Rabbit
(liquid, azide, NaCl, PBS, glycerol, affinity purified)
WB Human Immunogen = synthetic peptide based on the C-terminal end of human Calpain 5 (HtrA3) recognizes the longer forms of HtrA3 (Calpain 5), and not the shorter form -
Abcam polyclonal
IgG
Rabbit
(liquid, azide, NaCl, PBS, glycerol, affinity purified)
WB Human Immunogen = synthetic peptide based on the catalytic domain of human Calpain 5 (HtrA3) recognizes both isoforms of HtrA3 (Calpain 5) -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, PBS, glycerol, purified)
ELISA, IC, IH, WB Human Immunogen = purified human erythrocyte mu-calpain reacts with mu-calpain (Calpain I) large subunit -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, ascites, no preservative)
ELISA, IC, IH, IP, WB Bovine Immunogen = purified bovine myocardial mu-calpain reacts with the small subunit of human erythrocyte u-calpain or bovine myocardial m-calpain. Does not react with calpain large subunits -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, ascites)
ELISA, IC, IP, WB Human, Hamster, Mouse, Rat Immunogen = purified human erythrocyte Calpain-I large subunit calpain-I (mu-Calpain); no cross-reaction with human placental m-calpain (calpain II); ppts proteolytically active calpains. -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, ascites)
ELISA, IP, WB Bovine, Human, Mouse, Rat Immunogen = purified human placenta Calpain specific for the 80kD subunit of calpain I (mu-calpain). Two smaller proteins, presumed to be autolysis products, are also recognized. -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, ascites, azide)
IC, IP, WB Bovine, Porcine, Human, Rat Immunogen = purified bovine mu-calpain recognizes an epitope in domain II (aa 245-265) of mu-calpain (~80kDa); no cross-reaction with m-calpain, n-calpain, calmodulin or calpastatin -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, ascites, azide)
IC, IP, WB Bovine, Porcine, Human, Rat Immunogen = purified bovine mu-calpain recognizes an epitope in domain III (aa 465-520) of mu-calpain (~80kDa); no cross-reaction with m-calpain, n-calpain, calmodulin or calpastatin -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
ELISA, IP, WB Bovine, Human, Mouse, Rat Immunogen = purified bovine mu-calpain Calpain 1 (mu-calpain) -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, HEPES, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide derived from the human sequence of calpain 1 large subunit, KLH-coupled detects endogenous levels of total calpain 1 (large subunit) protein; detects full-length calpain 1 as well as calpain 1 autoproteolytically cleaved at leucine 28 -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(liquid, unpurified serum, PBS, glycerol, azide)
WB Human Immunogen = human Calpain I (large subunit) recognizes the large subunit (80kD) of human calpain I, not the smaller subunit -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(liquid, ascites, PBS, azide)
WB Bovine, Human Immunogen = purified 28kD calpain subunit from bovine skeletal muscle. recognizes 28kDa calpain subunit; no cross-reaction with the 80kD m-calpain subunit -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(liquid, PBS, BSA, thimersal, glycerol)
WB Bovine, Rabbit, Pig, Human, Mouse, Rat Immunogen = synthetic peptide surrounding aa700 of human Calpain I recognizes large subunit 80kDa Calpain 1; not the 28kD smaller subunit of Calpain 1 -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, unpurified serum)
ELISA, IC, IH, WB Human Immunogen = purified human placental m-calpain reacts with m-calpain (Calpain II) large subunit -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, BSA, thimerosal, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide mapping to the C-terminus of the domain III of human Calpain-2 large subunit recognizes the 80 kDa Calpain-2; no cross-reaction with other calpains -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, glycerol, azide, affinity purified)
ELISA, IH, IP, WB Human Immunogen = synthetic peptide based on Insert #2, in domain-II of Calpain-94 large subunit. Based on the human sequence. Reacts with the cysteine protease Calpain-94 (Calpain-3, nCANP, nCL-1, Calpain p94, muscle calpain). The rat 88 and 90kD novel calpains share the insert #1 and the antibody may cross-react with these forms. -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
ELISA, WB Human Immunogen = synthetic peptide corresp. to C-terminal residues of human CAPN3 (Calpain-3) Calpain-3 -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(liquid, PBS, BSA, glycerol, thimerosal, affinity purified)
WB Human Immunogen = synthetic peptide mapping to the domain I of human calpain-3 large subunit Calpain-3 (82-94 kDa and 60-62 kDa forms); no cross-reaction with other calpains -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(liquid, PBS, BSA, glycerol, thimerosal, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide mapping to the domain II of human calpain-5 large subunit Calpain-5 (73 kDa and 58 kDa forms as well as several smaller cleavage products); no cross-reaction with other calpains -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(liquid, PBS, BSA, glycerol, thimerosal, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide mapping to the domain I of human calpain-6 large subunit Calpain-6 (75 kDa and 58 kDa forms as well as several smaller cleavage products); no cross-reaction with other calpains -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(liquid, PBS, BSA, glycerol, azide, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide corresp. to aa residues in domain N (IV) of human Calpain-7 large subunit Calpain-7 (93 kDa and 58 kDa); no cross-reaction with other calpains -
LifeSpan Biosciences, Inc. polyclonal
IgY
Chicken
(liquid, PBS, no preservative, affinity purified)
ELISA, IH, WB Human Immunogen = recombinant protein Calpain inhibitor aa1-137 -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, ascites, glycerol, purified)
ELISA, IC, IH, WB Human Immunogen = human erythrocyte calpastatin purified Calpain inhibitor (calpastatin) -
LifeSpan Biosciences, Inc. monoclonal
IgG2a
Mouse
(liquid, ascites, PBS, azide)
IC, WB Bovine, Porcine, Human, Rat Immunogen = purified bovine skeletal muscle calpastatin recognizes an epitope in domain II (aa 295-501); Detects bands of ~150, ~125, ~90 and ~70kDa by WB, which have been suggested to correspond to protein isoforms translated from different start sites; no cross-reaction with calpains or calmodulin -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, ascites, PBS, azide)
IC, WB Bovine, Porcine, Human, Rat Immunogen = purified bovine skeletal muscle calpastatin recognizes an epitope in domain IV (aa 543-673); Detects bands of ~150, ~125, ~90 and ~70kDa by WB, which have been suggested to correspond to protein isoforms translated from different start sites; no cross-reaction with calpains or calmodulin -
Cell Signaling polyclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide)
IF, WB Human, Mouse, Rat Immunogen = synthetic peptide derived from the human sequence of calpain 1 (large subunit), KLH-coupled endogenous levels of total calpain 1 (large subunit) protein; detects full-length calpain 1 as well as calpain 1 autoproteolytically cleaved at leucine 28 -
Cell Signaling polyclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide, affinity purified)
FC, IF, IP, WB Human, Mouse, Rat Immunogen = synthetic peptide derived from the human sequence of calpain 2 (large subunit), KLH-coupled endogenous levels of total calpain 2 (large subunit) protein; detects full-length calpain 2 as well as calpain 2 autoproteolytically cleaved at serine 20; does not detect recombinant calpain 1 -
US Biological monoclonal
IgG1
Mouse
(liquid, ascites, PBS, azide)
WB Bovine, Human Immunogen = purified 28kD calpain subunit from bovine skeletal muscle detects a band of ~28kD of calpain by WB; no cross-reaction with the human 80kD m-calpain subunit -
US Biological monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide, affinity purified)
ELISA, FC Human Immunogen = recombinant human Calpain 7 calpain 7 -
US Biological monoclonal
IgG1
Mouse
(liquid, ascites, glycerol)
ELISA, IC, IP, WB Human, Hamster, Mouse, Rat Immunogen = Calpain-I large subunit from human erythrocyte calpain-I (mu-Calpain)no cross-reaction with human placental m-calpain (calpain II) -
US Biological monoclonal
IgG1
Mouse
(liquid, ascites, azide)
IC, WB Bovine, Pig, Human, Rat Immunogen = purified bovine µ-calpain epitope in domain III (aa 465-520) of µ-calpain 80kDa; no cross-reaction with m-calpain, n-calpain, calmodulin or calpastatin -
US Biological monoclonal
IgG1
Mouse
(liquid, ascites, azide)
IC, WB Bovine, Pig, Human, Rat Immunogen = purified bovine µ-calpain epitope in domain II (aa 245-265) of µ-calpain 80kDa; no cross-reaction with m-calpain, n-calpain, calmodulin or calpastatin -
US Biological polyclonal
IgG
Rabbit
(liquid, serum, PBS, BSA, azide)
WB Human Immunogen = human Calpain I (large subunit) recognizes the large subunit (80kD) of human calpain I; does not recognize the smaller subunit of calpain I -
US Biological monoclonal
IgG1
Mouse
(liquid, PBS, glycerol, ammonium sulfate ppt)
ELISA, IC, IH, WB Human Immunogen = human erythrocyte mu-calpain reacts with mu-calpain (Calpain I) large subunit -
US Biological polyclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic peptide derived from the human sequence of calpain 1 (large subunit) (KLH-coupled) detects endogenous levels of total calpain 1 (large subunit) protein; detects full-length calpain 1 as well as calpain 1 auto-proteolytically cleaved at leucine 28 -
US Biological monoclonal
IgG1
Mouse
ELISA, IP Bovine, Human, Mouse, Rat Immunogen = Calpain purified from human placenta specific for the 80kD subunit of calpain I (mu-calpain). Two smaller proteins, presumed to be autolysis products, are also recognized -
US Biological polyclonal
IgG
Rabbit
(liquid, no preservative, affinity purified)
ELISA, IC, IH, WB Human Immunogen = human placental m-calpain m-calpain (Calpain II) large subunit -
US Biological monoclonal
IgG1
Mouse
(liquid, ascites, no preservative)
ELISA, IC, IH, IP, WB Bovine, Human Immunogen = bovine myocardial mu-calpain reacts with the small subunit of human erthrocyte u-calpain or bovine myocardial m-calpain; not calpain large subunits -
US Biological polyclonal
IgG
Rabbit
(liquid, azide, affinity purified)
ELISA, IH, IP, WB Human Immunogen = synthetic peptide based on Insert #2, in domain-II of human Calpain-94 large subunit reactive with the cysteine protease Calpain-94 (Calpain-3, nCANP, nCL-1, Calpain p94, muscle calpain); may crossreact with rat 88 and 90kD novel calpains at the insert #1 -
Novus Biologicals monoclonal
IgG1
Mouse
(liquid ascites, azide)
IC, IF, WB Cow, Pig, Human, Rat immunogen = purified bovine skeletal muscle 80 kDa Mu Calpain subunit (amino acids 465-520) recognizes an epitope between amino acids 465-520 (domain III) of human Mu Calpain; in WB, detects an 80 kDa protein -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, antisera, PBS, BSA, azide, affinity purified)
IF, IP, WB Dog, Human, Mouse immunogen = synthetic peptide: R G E R T A F I K D Q S A L, corresp. to aa780-793 of human Furin Convertase detects Furin convertase from canine and mouse cells as well as transfected human Furin; does not detect endogenous Furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells nor from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissue -
Acris Antibodies polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified, unconjugated)
IC, IF, IP, Neut, WB Dog, Human, Mouse immunogen = synthetic peptide: R G E R T A F I K D Q S A L, corresp. to aa780-793 of human Furin Convertase detects Furin convertase from canine and mouse cells as well as transfected human Furin; does not detect endogenous Furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells nor from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissue -
Abcam polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, BSA, azide)
IC, IF, IP, WB Dog, Human, Mouse, Rat immunogen = synthetic peptide: R G E R T A F I K D Q S A L, corresp. to aa780-793 of human Furin detects furin convertase from canine and mouse cells as well as transfected human furin; does not detect endogenous furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells nor from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissue -
A B R - Thermo Scientific polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified IgG, unconjugated)
IC, IF, IP, WB Dog, Human, Mouse immunogen = synthetic peptide: R G E R T A F I K D Q S A L, corresp. to aa780-793 of human Furin Convertase detects Furin convertase from canine and mouse cells as well as transfected human Furin; does not detect endogenous Furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells nor from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissue -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
IF, IP, WB Human Immunogen = humanerythrocyte Calpastatin detects Calpastatin -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
IF, IP, WB Human Immunogen = humanerythrocyte Calpastatin detects Calpastatin, 126 kDa -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, IP, WB Mouse, Rat Immunogen = peptide mapping at the C-terminus of Calpastatin of rat origin detects Calpastatin, 126 kDa -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, IP, WB Human Immunogen = peptide mapping at the C-terminus of Calpastatin of human origin detects Calpastatin, 126 kDa -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
ELISA, IF, IH, IP, WB Human, Mouse, Rat Immunogen = amino acids409-708 mapping at the C-terminus of Calpastatin of human origin detects Calpastatin, 126 kDa -
Santa Cruz monoclonal
IgG2b
Mouse
(liquid, PBS, azide, protein stabilizer)
FC, IP, WB Human immunogen = raised against aa108-715 of human Furin Convertase Furin -
Abcam polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, sucrose, azide)
WB Human, Mouse, Rat immunogen = synthetic peptide based on the N-terminal end of mature human furin recognizes subtilisin-like serine protease furin -
Abcam polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, sucrose, azide)
WB Human, Mouse, Rat immunogen = synthetic peptide based on the Homo-B domain of human furin recognizes subtilisin-like serine protease furin -
Abcam polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, BSA, azide)
WB Rabbit, Cow, Sheep, Human, Mouse, Rat immunogen = synthetic peptide derived from within residues 100-200 of human Furin, conj. to KLH furin -
Abcam monoclonal
IgG1
Mouse
(liquid, PBS, no preservative, protein G purified)
IH, WB Human immunogen = recombinant human Furin furin -
EMD Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, ascites, PBS, azide)
IB, IC, WB Bovine, Human, Rat Immunogen = purified calpain-1 (µ form) from bovine skeletal muscle; epitope maps within aa 245-265 of calpain-1 domain II recognizes the ~80 kDa latent form of the large subunit of calpain-1 -
EMD Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, undiluted ascites, azide)
IB, IC, WB Bovine, Pig, Human, Rat Immunogen = purified calpain-1 (µ form) from bovine skeletal muscle; epitope maps within aa 465-520 of calpain-1 domain III recognizes the ~80 kDa latent form of the large subunit of calpain-1 -
EMD Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, PBS, azide, glycerol)
ELISA, IB, IP Bovine, Human Immunogen = purified calpain from human placenta recognizes the ~28-30 kDa subunit of µ-calpain (calpain-1) and m-calpain (calpain-2). Recognizes both native and denatured forms of the small subunit of calpain. -
EMD Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, ascites, PBS, azide)
IB, IC, IP Bovine, Porcine, Human, Rat Immunogen = purified calpain-2 from bovine skeletal muscle; epitope within aa502-699 of calpain 2 domains III/IV recognizes the ~80 kDa subunit of calpain-2 -
Abcam monoclonal
IgG2b
Mouse
(liquid, supernatant, azide)
IH Human immunogen = recombinant full length protein (human) Cathepsin B -
Abcam polyclonal
IgG
Rabbit
(liquid, tris-glycine saline, glycerol; protein A purified)
IH, WB Mouse, Rat immunogen = recombinant rat pro Cathepsin B1 recognizes Procathepsin B (~40kDa) and mature Cathepsin B (25kD, 26kD and 30kD -
Abcam polyclonal
IgG
Rabbit
ELISA, WB Human immunogen = full length native protein (purified): Cathepsin B from human liver recognises liver Cathepsin B; no cross-reaction with Cathepsin L or Cathepsin H. -
Abcam polyclonal
IgG
Sheep
(liquid, EACA, benzamidine, glycine buffered saline, EDTA)
WB Human immunogen = human Cathepsin B purified from liver recognises human Cathepsin B, a lysosomal cysteine protease of 29kD -
Abcam polyclonal
IgG
Sheep
(liquid, PBS, azide; conj. FITC)
IF Human immunogen = full length native purified protein from human placenta Cathepsin B -
Abcam polyclonal
IgG
Sheep
(liquid, PBS, no preservative; conj. HRP)
ELISA Human immunogen = full length native human purified Cathepsin B protein from placenta Cathepsin B -