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Antibodies

1284 Results

Name Source Clonality &
Isotype
Host &
Formulation
Method Reactivity Immunogen/
Epitope
Specificity Availability
MAPKs From manufacturers (100)
tebu-bio polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified, also available as rabbit sc-16564-R)
IF, WB Human, Mouse, Rat Immunogen = short amino acid sequence containing phosphorylated Thr 218 and Tyr 220 of human ERK 5 ERK 5 phosphorylated at Thr 218/Tyr 220 -
tebu-bio polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
IF, WB Human, Mouse, Rat Immunogen = peptide mapping at the C-terminus of human ERK 6 ERK 6 -
tebu-bio polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
IF, WB Human, Mouse, Rat Immunogen = peptide mapping at the N-terminus of human ERK 6 ERK 6 -
tebu-bio monoclonal
IgG2a
Mouse
(liquid, PBS, gelatin, azide, available conj. phycoerythrin sc-7838 PE, agarose sc-7838 AC, fluorescein sc-7383 FITC or rhodamine sc-7383 TRITC)
IF, IH, IP, WB Human, Mouse, Rat Epitope corresponding to a sequence containing phosphorylated Tyr-204 of human ERK ERK1 and ERK2 phosphorylated at Tyr-204 -
tebu-bio polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified, available conj. to phycoerythrin sc-7976 PE or as rabbit ab sc-7976-R)
FC, IF, IP, WB Zebra fish, Human, Mouse, Rat, Xenopus, C. elegans, Drosophila Immunogen = short amino acid sequence containing phosphorylated Tyr 204 of human ERK ERK phosphorylated Tyr204 -
tebu-bio polyclonal
IgG
Goat
(liquid, PBS, BSA, gelatin, azide, affinity purified)
IF, WB Human, Mouse, Rat Immunogen = short aa sequence containing phosphorylated Thr185 and Tyr 187 of human ERK1/2 ERK1 phosphorylated at Tyr-202/204 and ERK2 phosphorylated at Tyr-185/187 -
tebu-bio kit
Mouse
ELISA Human, Mouse, Rat - measurs the relative amount of ERK1/2 (Thr202/Tyr204) phosphorylationand screens the effects of various treatments, inhibitors or activators -
Sigma Aldrich monoclonal
IgG1
Mouse
(liquid, PBS, azide)
DB, ELISA, IB, IH Human, Mouse, Rat immunogen = synthetic peptide seq containing the 11 aa H T G F L T E Y V A T, corresp. to the non-phosphorylated form of ERK-activation loop, conj. to KLH MAP kinase (ERK-1 (44 kDa) and ERK-2 (42 kDa) containing threonine residue; not MAPK, JNK or p38-MAPK -
Sigma Aldrich monoclonal
IgG1
Mouse
(liquid, PBS, azide)
ELISA, IB, IC Human, Rat immunogen = synthetic peptide containing 11 aa H T G F L T E Y V A T, corresp. to the non-phosphorylated form of ERK-activation loop, conj. to KLH non-phosphorylated, non-activated form of MAP kinase (ERK1, 44kDa and ERK2, 42 kDa); weak reaction seen with mono-phosphorylated thr or tyr, but not with double-phosphorylated peptides of MAPK. No reaction with JNK or p38 MAPK -
Sigma Aldrich kit
Unknown Host
ELISA Human, Mouse, Rat - quantitation of ERK1/2 doubly phosphorylated at thr185 and tyr187 in cell lysates -
Sigma Aldrich monoclonal
IgG1
Mouse
(liquid, PBS, azide)
DB, ELISA, IB, IC Human, Rat immunogen = synthetic peptide containing 11 aa H T G F L T E pY V A T, corresp. to the phosphorylated form of ERK-activation loop, conj. to KLH monophosphorylated tyrosine form of MAP kinase (ERK1 and ERK2) and JNK; no cross-reaction with non-phosphorylated, the doubly phosphorylated and the mono-phosphorylated threonine forms of the MAPK molecule and the doubly phosphorylated form of JNK or p38 MAPK -
Sigma Aldrich monoclonal
IgG1
Mouse
(liquid, azide, purified)
WB Bovine, Yeast, Human, Hamster, Mouse, Rat, Xenopus, C. elegans immunogen = synthetic peptide H T G F L pT E pY V A T corresp. to the phospho. form of the ERK activation loop active dually-phospho. ERK2 (42kDa) and ERK1 (44 kDa) -
Sigma Aldrich monoclonal
IgG1
Mouse
(aqueous solution, PBS, azide, purified, KLH conj)
ELISA, IC, IH, IP, WB Bovine, Yeast, Human, Hamster, Mouse, Rat, Xenopus, C. elegans immunogen = synthetic peptide H T G F L pT E pY V A T corresp. to the phospho. form of the ERK activation loop active dually-phospho. ERK2 (42kDa) and ERK1 (44 kDa) -
Sigma Aldrich monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide, purified, FITC conj.)
IF Bovine, Yeast, Human, Hamster, Mouse, Rat, Xenopus, C. elegans, Drosophila immunogen = synthetic peptide H T G F L pT E pY V A T corresp. to the phospho. form of the ERK activation loop active dually-phospho. ERK2 (42kDa) and ERK1 (44 kDa) -
Zymed/ Invitrogen polyclonal
Rabbit
(liquid, PBS, BSA, azide, concentrate)
IH - immunogen = synthetic phosphopeptide corresp. to the region surrounding the phosphorylated Thr202 and Tyr204 residues of ERK1/2 (p44/p42 MAP kinase) ERK1/2 phos Thr202/Tyr204 -
Zymed/ Invitrogen polyclonal
Rabbit
(liquid, pre-diluted, PBS, BSA, azide; also available as concentrate, 18-2389)
IH - immunogen = synthetic phosphopeptide corresp. to the region surrounding the phosphorylated Thr202 and Tyr204 residues of ERK1/2 (p44/p42 MAP kinase) ERK1/2 phos Thr202/Tyr204 -
Zymed/ Invitrogen polyclonal
Rabbit
(liquid, PBS, azide, affinity purified)
ELISA, IH, IP, WB Human, Mouse, Rat immunogen = synthetic peptide corresp. to a sequence near the C-terminus of rat ERK2 ERK2; minimal cross with ERK1 -
Zymed/ Invitrogen monoclonal
IgG1
Mouse
(liquid, ascites, PBS, azide, purified)
IP, WB - immunogen = recomb. ERK2 ERK2; minimal or no reactivity with ERK1 -
Novus Biologicals polyclonal
IgG
Goat
(liquid, tris-saline, BSA, azide, purified)
ELISA, WB Bovine, Chicken, Chimpanzee, Human, Mouse, Xenopus immunogen = synthetic peptide C A A G P E M V R G Q V F, corresp. to the internal region of the protein; NP_002736; NP_620407 ERK2/MAPK1 -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide)
ELISA, IH, IP, WB Human, Mouse, Rat immunogen = synthetic peptide corresponding to the C-terminal of rat ERK1 protein. ERK1 -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, PBS, BSA, glycerol, azide)
FC, IH, WB Human immunogen = synthetic peptide derived from the region of human ERK 1 + 2 that contains threonine 202/185 and tyrosine 204/187 ERK1 + 2 phospho Thr185/Tyr202 -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, no preservative)
WB Human, Mouse, Rat immunogen = synthetic phospho- peptide corresp. to residues Thr202 /Tyr204 of human p44 MAP kinase (Erk1), conj. KLH ERK1 phosphorylated at Thr202 and Tyr204 and ERK1 phosphorylated at Thr183 and Thr185. -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, glycerol, PBS, BSA, azide, affinity purified)
WB Cow, Human, Mouse, Rat immunogen = synthetic peptide, corresp. to C- terminal aa 317-339 of human ERK1 + ERK2 ERK1 + ERK2 -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
IH Human, Mouse, Rat Immunogen = synthetic peptide to C-terminal of rat ERK1(unfortunately, the amino acid sequence is considered to be commercially sensitive) ERK1 -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, BSA, azide, affinity purified)
IF, WB Human Immunogen = synthetic peptide derived from the region of human ERK 1 + 2 containing thr 202/185 and tyr 204/187 ERK1 and ERK2 (phospho T185 + T202) -
Abcam monoclonal
IgG
Rabbit
(liquid, protein-A, BSA, Tris, glycerol, azide)
FC, IC, IH, IP, WB Human, Mouse, Rat Immunogen = synthetic peptide corresp. to residues near the C terminal of human ERK2 reacts with an epitope located in the C terminal region of ERK2 -
Abcam monoclonal
IgG
Rabbit
(liquid, protein-A, BSA, glycerol, Tris, azide)
FC, IC, IP, WB Human, Mouse, Rat Immunogen = synthetic peptide, corresp. to residues in the C-terminus of Human ERK5 ERK5 -
Abcam polyclonal
IgG
Goat
(liquid, tris buffered saline, BSA, azide, affinity purified)
ELISA, WB Human Immunogen = synthetic peptide: C A A G P E M V R G Q V F corresp. to aa 9-19 of human ERK2 ERK2 / MAPK1 -
Abcam monoclonal
IgG
Rabbit
(liquid, tris-glycine saline, BSA, glycerol, azide)
WB Human, Mouse, Rat immunogen = synthetic peptide corresponding to residues in C-terminus of human Erk1 detects Erk1 and is predicted to react with Erk1c based on sequence homology -
Abcam monoclonal
IgG
Rabbit
(liquid, tris-glycine saline, glycerol, azide, protein A purified)
FC, IC, IH, IP, WB Human, Mouse immunogen = synthetic peptide corresponding to N- terminus of human ERK1 ERK1; not other MAP kinase -
Abcam polyclonal
IgG
Sheep
(liquid, tris-glycine saline, glycerol, azide, affinity purified)
IP, WB Human, Mouse, Rat immunogen = synthetic peptide: C T G F L T E [pY] V A T, corresp. to aa 174-183 of human ERK1 + ERK2 recognises phosphorylated Erk1 and Erk2 -
Abcam polyclonal
IgY
Chicken
(liquid, PBS, no preservative, affinity purified)
WB Human, Mouse, Rat immunogen = synthetic peptide, corresp. to aa 2-140 of human ERK5 ERK5 -
Abcam polyclonal
IgY
Chicken
(liquid, PBS, no preservative, affinity purified)
WB Human immunogen = corresponds to aa 341-570 of human ERK5 ERK5 -
Abcam polyclonal
IgY
Chicken
(liquid, PBS, no preservative, affinity purified)
ELISA Human immunogen = fusion protein: G G E P R R T E G V G P G V P G E V, corresp. to aa 11-28 of human ERK1 ERK1 -
Abcam monoclonal
IgG2b, κ
Mouse
(liquid, PBS, glycerol, no preservative)
WB Human, Mouse, Rat immunogen =synthetic peptide corresp. to residues around Thr202/Tyr204 of human ERK1, KLH coupled ERK1+2 -
Abcam polyclonal
IgG
Sheep
(liquid, PBS, azide)
IP Human, Rat immunogen = recombinant p42/p44 MAP kinase protein ERK1+2 -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, no preservative, affinity purified)
ELISA, IP, WB Human immunogen = synthetic peptide human p42 ERK1 C-terminal peptide, conj. to KLH p42 MAPK (ERK2), no cross-reaction with p44 MAPK (ERK2) -
Abcam polyclonal
IgG
Goat
(liquid, BSA, TBS, azide, affinity purified)
ELISA, WB Human Immunogen = synthetic peptide: C-G G E P R R T E G V G P, corresp. to N terminal aa 11-22 of human ERK1 ERK1; no cross reactivity is expected with ERK2 -
GeneTex polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
IH Human, Mouse, Rat Immunogen = synthetic peptide to C-terminal of rat ERK1(unfortunately, the aa sequence is considered to be commercially sensitive) ERK1 -
Sigma Aldrich monoclonal
IgG1
Mouse
(buffered aqueous solution, PBS, azide, purified)
ELISA, IC, WB Human, Rat immunogen = synthetic peptide H T G F L pT E pY V A T corresp. to the phospho. form of the ERK activation loop active monophospho. (Thr 183) and doubly phospho. forms of ERK2 (42kDa) and ERK1 (44 kDa) -
Biosource Invitrogen monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide, affinity purified)
ELISA, WB Human, Mouse, Rat immunogen = chemically synthesized peptide derived from the ERK2 sequence, containingphosphorylated residues corresponding to threonine 185 and tyrosine 187 (threonine 202and tyrosine 204 in ERK1) phosphorylated ERK1 and ERK2 -
Sigma Aldrich monoclonal
IgG2a
Mouse
(liquid, PBS, azide, protein G purified)
IP, WB Human, Mouse, Rat immunogen = purified recombinant mouse MAPK2 MAPK2 (42kDa); may weakly react with MAPK1 (ERK1) and only binds denatured MAP kinase -
Sigma Aldrich polyclonal
IgG
Rabbit
(liquid, tris-glycine saline, azide, protein G purified)
IP, WB Human, Mouse, Rat immunogen = purified recombinant mouse MAP kinase 2 MAP kinase 2 (42 kDa, ERK2). No cross reaction with MAP kinase (ERK1) or interference with kinase activity. -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
IF, WB Human, Mouse, Rat Immunogen = short aa sequence containing phosphorylated Thr177 of human ERK1/2 ERK and ERK2 phosphorylated at Thr177 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
IF, WB Human, Mouse, Rat Immunogem= short aa sequence containing phosphorylated Thr 177 and Thr 160 of human ERK2 ERK 1 and ERK2 phosphorylated Thr177 and Thr 160 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, BSA, gelatin, azide, affinity purified)
IF, WB Human, Mouse, Rat Immunogen = short aa sequence containing phosphorylated Thr185 and Tyr 187 of human ERK1/2 ERK1 phosphorylated at Tyr-202/204 and ERK2 phosphorylated at Tyr-185/187 -
Santa Cruz monoclonal
IgG2a
Mouse
(liquid, PBS, azide, available conjugated to phycoerythrin sc-1647 PE, agarose sc-1647 AC or HRP sc-1647 HRP)
FC, IF, IH, IP, WB Human, Mouse, Rat Immunogen = epitope mapping at the C-terminus of human ERK 2 MAP kinase p42 ERK 2 p42 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide, affinity purified, also available conjugated to phycoerythrinsc-155 PE)
FC, IF, IP, WB Human, Mouse, Rat Immunogen = peptide mapping subdomain XI (r) of rat ERK3 ERK3 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide, affinity purified, available as phycoerythrin conj. sc-156 PE)
FC, IF, IH, IP, WB Human, Mouse, Rat Immunogen = peptide mapping within an internal region of rat ERK3 ERK3 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide, available as phycoerythrin conjugate sc-10787 PE)
FC, IF, IP, WB Human, Mouse, Rat Immunogen = amino acids 565-721 mapping at the C-terminus of human ERK3 ERK3 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
IF, WB Human, Mouse, Rat Immunogen = shortamino acid sequence containing phosphorylated Ser 189 of ERK 3 of human origin ERK3 phosphorylated at Ser 189 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide, available as phycoerythrin conjugate sc-5626 PE)
FC, IF, IP, WB Human, Mouse, Rat Immunogen = amino acids 416-815 mapping at the C-terminus of human ERK 5 ERK5 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified, available as phycoerythrin conj. sc-1284 PE or agarose conj. sc-1284 AC)
FC, IF, IP, WB Human, Mouse, Rat Immunogen = peptide mapping at the C-terminus of human ERK 5 ERK 5 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
IF, IP, WB Human, Mouse, Rat Immunogen = peptide mapping at the N-terminus of human ERK 5 ERK 5 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
IF, IP, WB Human, Mouse, Rat Immunogen = peptide mapping near the C-terminus of human ERK 5 ERK 5 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified, also available as rabbit sc-16564-R)
IF, WB Human, Mouse, Rat Immunogen = short amino acid sequence containing phosphorylated Thr 218 and Tyr 220 of human ERK 5 ERK 5 phosphorylated at Thr 218/Tyr 220 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
IF, WB Human, Mouse, Rat Immunogen = peptide mapping at the C-terminus of human ERK 6 ERK 6 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
IF, WB Human, Mouse, Rat Immunogen = peptide mapping at the N-terminus of human ERK 6 ERK 6 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified, available conj. to phycoerythrin sc-7976 PE or as rabbit ab sc-7976-R)
FC, IF, IP, WB Zebra fish, Human, Mouse, Rat, Xenopus, C. elegans, Drosophila Immunogen = short amino acid sequence containing phosphorylated Tyr 204 of human ERK ERK phosphorylated Tyr204 -
Accurate polyclonal
Rabbit
(IgG fraction)
- - - - -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, buffered solution, BSA, azide)
IC, IH, IP, WB Bovine, Dog, Chicken, Human, Mouse, Rat, Xenopus Immunogen = synthetic peptide corresponding to positions 325-345 of rat ERK1 ERK1 -
Chemicon and Upstate are Millipore monoclonal
IgG
Rabbit
(liquid, Tris-glycine, NaCl, glygerol, azide, protein A purified)
IP, WB Mouse, Rat immunogen = synthetic peptide corresponding to rat Erk1, amino acids 346-380 (CGGPFTFDME LDDLPKERLK ELIFQETARFQ PGAPEAP) Recognizes MAP Kinase 1/Erk1 (44kDa) -
Chemicon and Upstate are Millipore kit
Unknown Host
(derived from a bovine source; Fluorescence Polarization using a phosphorylated peptide labeled with a green dye)
IF - - ERK1 -
Chemicon and Upstate are Millipore polyclonal
Rabbit
(liquid, borate buffered saline, affinity purified)
ELISA, IC, IH, IP, WB Chicken, Mollusk, Sea star, Sea Urchin, Sheep, Human, Mouse, Rat, Xenopus Immunogen = synthetic peptide aa. 333-367 of rat ERK1, conjugated to KLH Recognizes ERK1 and ERK2 (~42 kDa and ~44 kDa, respectively). -
Chemicon and Upstate are Millipore polyclonal
IgG
Rabbit
(liquid, PBS, azide, conj. to protein A agarose by dimethyl-pimelimidate)
IP, ISP Chicken, Sea star, Human, Mouse immunogen = synthetic peptide of 38 residues (CGGPFTFDM ELDDLPKERL KELIFQETAR FQGAPEAP) to the C-terminal of rat Kinase2/ERK2 ERK1/2 MAP kinase -
Chemicon and Upstate are Millipore polyclonal
IgG
Rabbit
(liquid, PBS, BSA, Tween, azide, affinity purified, Alexa Flour® 488-conj.)
FC, IC, IF, WB Chicken, Human, Mouse, Rat immunogen = 38 residue synthetic peptide (CGGPFTFDM ELDDLPKERL KELIFQETAR FQGAPEAP) corresp. to the C-terminal 35 amino acids of the rat 44kDa MAP Kinase 2/Erk2, coupled to KLH Recognizes MAPK1/Erk1 (44kDa) and MAPK2/Erk2 (42kDa) -
Chemicon and Upstate are Millipore polyclonal
IgG
Rabbit
(liquid, affinity purified, Alexa Flour® 555-conj.)
FC, IC, IF, WB Chicken, Human, Mouse, Rat immunogen = 38 residue synthetic peptide (CGGPFTFDM ELDDLPKERL KELIFQETAR FQGAPEAP) corresp. to the C-terminal 35 amino acids of the rat 44kDa MAP Kinase 2/Erk2, coupled to KLH Recognizes MAPK1/Erk1 (44kDa) and MAPK2/Erk2 (42kDa) -
Chemicon and Upstate are Millipore kit
Rabbit
(measures the phospho transferase activity of MAP Kinase in immuno precipitates and column fractions)
IP - - ERK1/2 -
Chemicon and Upstate are Millipore kit
Rabbit
(measures phosphotransferase activity in an immunocomplex formed between the MAPK R2 antibody and MAPK (p44mpk). This precipitated enzyme is used to phosphorylate MBP substrate)
IP Chicken, Sea star, Human, Mouse - ERK1/2 -
Chemicon and Upstate are Millipore monoclonal
IgG
Rabbit
(liquid, Tris-glycine, NaCl, glycerol, azide, protein A purified)
WB Human, Rat immunogen = peptide corresp. to aa surrounding the pT E pY motif of human MAP Kinase 1/2 (Erk1/2), with phospho Thr185 and Tyr 187 recognizes phospho-MAP kinase 1 (ERK1, 44kDa) and phospho-MAP kinase 2 (ERK2, 42kDa) -
Chemicon and Upstate are Millipore polyclonal
IgG
Sheep
(liquid, Tris-glycine, NaCl, glycerol, azide, affinity purified)
IP, WB Human, Mouse, Rat immunogen = synthetic peptide (CTGFLTE pYVAT) corresp. to residues 174-183 of human MAP Kinase recognizes phosphorylated Erk1 and Erk2 -
Chemicon and Upstate are Millipore polyclonal
IgG
Rabbit
(liquid, tris-glycine, NaCl, glycerol, azide, protein A purified)
WB Human, Rat immunogen = synthetic peptide corresp. to aa surrounding the pTEpY motif of human phospho-MAP Kinase 1/2 (Erk1/2), coupled to KLH Recognizes phospho-MAP Kinase 1 (Erk1), 44 kDa and phospho-MAP Kinase 2 (Erk2) 42 kDa -
Chemicon and Upstate are Millipore polyclonal
IgG
Rabbit
(frozen solution, tris-glycine, NaCl, EDTA, affinity purified)
IC, IP, WB Avian (various), Echinoderm, Sheep, Human, Mouse, Rat, Xenopus immunogen = 38 residue synthetic peptide (CGGPFTFDM ELDDLPKERL KELIFQETAR FQGAPEAP) corresp. to the C-terminal 35 amino acids of the rat 44kDa MAP Kinase 2/Erk2 Recognizes MAPK1/Erk1 (44kDa) and MAPK2/Erk2 (42kDa) -
Chemicon and Upstate are Millipore polyclonal
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified)
WB Human, Mouse, Rat Immunogen = synthetic phosphopeptide corresp. to aa residues surrounding the phosphorylated Thr202/Tyr204 of ERK1 Recognizes the activated form of ERK1 and ERK2 (phospho at Thr202 and Tyr204 inhuman ERK1 and Thr185 and Tyr187 in human ERK2) -
Chemicon and Upstate are Millipore polyclonal
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified)
WB Human, Mouse, Rat, Xenopus Immunogen = synthetic phosphopeptide corresp. to aa residues surrounding the phosphorylated Thr202/Tyr204 of ERK1 and Thr185 and Tyr187 in human ERK2 Recognizes the activated form of ERK1 and ERK2 (phospho at Thr202 and Tyr204 inhuman ERK1 and Thr185 and Tyr187 in human ERK2) -
Chemicon and Upstate are Millipore monoclonal
IgG2a
Mouse
(frozen solution, PBS, azide, purified)
IP, WB Avian (various), Human, Mouse, Rat immunogen = HPLC purified recombinant MAP kinase 2 recognizes MAP Kinase encoded by the mapk gene. -
Chemicon and Upstate are Millipore polyclonal
IgG
Rabbit
(frozen solution, tris-glycine, NaCl, azide, protein G purified)
IP, WB Human, Mouse, Rat immunogen = recombinant mouse ERK2 recognizes MAP Kinase encoded by the mapk gene -
Chemicon and Upstate are Millipore kit
Unknown Host
(derived from bovine source, Fluorescence Polarization, phosphorylated peptide labeled with a FarRed dye)
IF - - ERK2 -
Chemicon and Upstate are Millipore kit
Unknown Host
(derived from bovine source, Fluorescence Polarization, phosphorylated peptide labeled with a green dye)
IF, IP - - ERK2 -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, PBS, azide, glycerol, purified)
ELISA, IC, IP, WB Avian (various), Dog, Human, Mouse, Rat Immunogen = phosphopeptide containing the pTEpY motif of Erk1 and Erk2, conjugated to KLH Recognizes phosphorylated threonine and tyrosine of Erk1 and Erk2. Does not cross-react with activated SAP Kinase 1 or 2 -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(PBS, azide, affinity purified)
IH Human Immunogen = synthetic peptide corresp. to the internal domain of human ERK-1 protein, KLH conj. ERK-1 -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(PBS, azide, affinity purified)
IH Human Immunogen = synthetic peptide corresp. to the internal domain of human ERK-1 protein, KLH conj. ERK-1 -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(protein A purified)
ELISA, IC, IF, IH, WB Zebra fish, Human, Hamster, Mouse, Rat Immunogen = synthetic peptide derived from the seq. of rat p42 MAP kinase, KLH conj. detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. In some cell types, recognizes p42 MAPK more readily than p44 MAPK; not JNK /SAPK nor p38 MAP kinase -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(DEAE-column chromatography)
ELISA, IH, IP, WB Human, Mouse, Rat Immunogen = synthetic peptide 15aa (aa 339-353) corresp. to a C-terminal segment of rat ERK1 Erk1/Erk2 -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
ELISA, IC, IP, WB Chicken, Frog, Sheep, Human, Mouse, Rat, Drosophila Immunogen = synthetic peptide corresp. to aa. 333-367 of rat Erk1 (KLH coupled) Erk1/Erk2 -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(ascites)
ELISA, FC, IC, IF, IH, IP, WB Zebra fish, Human, Hamster, Mouse, Rat Immunogen = synthetic phospho-peptide corresp. to residues around Thr202/Tyr204 of human p44 MAP kinase, (KLH coupled) detects endogenous levels of p44 and p42 MAP kinase (Erk1 and Erk2) dually phosphorylated at thr202 and tyr204; no crossreaction with the corresp. phosphorylated residues of either SAPK/JNK or p38 MAP kinase -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(unpurified serum, FITC conj.)
ELISA, FC, WB Human Immunogen = synthetic phospho-peptide derived from the region of ERK1/2 that contains thr202/185 and tyr204/187, based on the human seq. ERK1, endogenous and active forms -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(DEAE-column chromatography)
IC, IH, WB Human, Rat Immunogen = synthetic phospho-peptide derived from the region of human ERK1/2 pERK1 (~42kD) and pERK2 (~44kD) -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(DEAE-column chromatography)
IH, IP, WB Bovine, Chicken, Sheep, Human, Mouse, Rat, Xenopus, Mussel, Drosophila Immunogen = a 35 residue synthetic peptide, corresp. to aa 333-367 ERK-1 and ERK-2 -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(DEAE-column chromatography)
ELISA, IP, WB Rat Immunogen = synthetic peptide (aa324-345) corresp. to a segment near the C-terminus of rat ERK1 ERK1 and ERK2 -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(affinity purified)
ELISA, IP, WB Human, Mouse, Rat Immunogen = synthetic peptide corresponding to the F L pT E pY V A motif of phosphorylated ERK1/2 (MAPK2/3) recognizes the F L pT E pY V A motif of activated ERK1 and ERK2 when phosphorylated at both threonine and the tyrosine. No cross-reaction with non-phosphorylated forms of the protein and does not react with activated JNK or p38. -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(affinity purified)
ELISA, IP, WB Human Immunogen = synthetic peptide corresponding to the F L pT E pY V A motif of phosphorylated ERK1/2 (MAPK2/3) recognizes the F L pT E pY V A motif of activated ERK1 and ERK2 when phosphorylated at both threonine and the tyrosine. No cross-reaction with non-phosphorylated forms of the protein and does not react with activated JNK or p38. -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(HEPES, NaCl, BSA, glycerol, protein A and affinity purified)
IH, WB Human, Rat Immunogen = synthetic peptide detects levels of p42 and p44 Erk1 and Erk2 when phosphorylated at Thr202 and Tyr204 -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(affinity purified)
ELISA, IP, WB Bovine, Rabbit, Human, Mouse, Rat Immunogen = synthetic peptide derived from the C-terminal end of human p44 ERK ERK1 -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
IH, IP, WB Human, Mouse, Rat - ERK1 -
LifeSpan Biosciences, Inc. polyclonal
Goat
(affinity purified)
ELISA, WB Human Immunogen = synthetic peptide corresp. to the N- terminus of ERK1 / MAPK3: peptide sequence (G G E P R R T E G V G P) ERK-1; no cross-reactivity expected with ERK2 -
LifeSpan Biosciences, Inc. polyclonal
Chicken
(affinity purified)
ELISA, WB Human Immunogen = synthetic peptide ERK-1 -
LifeSpan Biosciences, Inc. monoclonal
Rabbit
(supernatant)
ELISA, FC, IC, IP, WB Human Immunogen = synthetic phosphopeptide corresp. to residues near the N-terminus of human ERK1 ERK1 -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(unpurified serum)
ELISA, WB Bovine, Human, Mouse, Rat immunogen = synthetic peptide derived from aa 317-339 within the C-terminal half of the human ERK1 protein ERK1 -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(unpurified serum)
ELISA, WB Human immunogen = synthetic phosphopeptide derived from the region of human ERK1&2 that contains threonine 202/185 and tyrosine 204/187 ERK1/2 when phosphorylated on threonine 202/185 and tyrosine 204/187 -