|
|
QED Bioscience |
monoclonal
IgG2a |
Mouse
(liquid, protein-G purified, TBS, no stabilizers or preservatives) |
ELISA, IH |
- |
immunogen = 8-hydroxy guanosine (oh8G)-BSA and -casein conjugates |
recognizes markers of oxidative damage to DNA and RNA |
- |
|
|
Calbiochem EMD Biosciences |
polyclonal
IgG |
Rabbit
(liquid, serum, diluted in glycerol, no preservative) |
ELISA, IH, WB |
Various species |
immunogen = HNE-treated KLH in which 1:1 amino acid-HNE Michael adducts werechemically reduced |
recognizes, specifically,chemically reduced amino acid-(4)-HNE adducts; not non-reduced adducts and reduced modifications arising frommalondialdehyde (MDA) or structurally-related alkenals such as trans-2-noneal |
- |
|
|
Lab Frontier |
monoclonal
IgG2b,κ |
Mouse
(liquid IgG, PBS, glycerol) |
ELISA, IP, WB |
Human |
immunogen = recomb. human protein purified from E.coli |
~10 kDa TRX-1 |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
IgG |
Rabbit
(liquid, Tris, NaCl, azide, glycerol, affinity purified) |
IC, WB |
Human, Rat |
immunogen = peptide corresp. to aa 137-150 (C-PHRIKAT NNKGKEK) of human thioredoxin reductase 1 |
~58 kDa thioredoxin reductase 1 |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(liquid, purified, azide) |
IH, WB |
Human, Mouse |
immunogen = nitrated KLH |
Reacts with nitrotyrosine |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(liquid, antiserum, azide) |
ELISA, IH |
Non-specific |
immunogen = NO2- Tyrosine- Glutaraldehyde- BSA |
NO2-Tyrosine |
- |
|
|
Chemicon and Upstate are Millipore |
kit
|
Unknown Host
(chemiluminescent detection, 96-well plates, nitrated BSA standard, nitrotyrosine antibody) |
ELISA |
Non-specific |
- |
for the quantitation of tyrosine nitration |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG2b |
Mouse
(liquid, purified Ig, azide) |
IH, WB |
Non-specific |
immunogen = nitrated KLH |
reacts with nitrotyrosine |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG2b,κ |
Mouse
(liquid, protein-G purified, Tris, NaCl, azide) |
IC, IH, IP, WB |
Non-specific |
immunogen = nitrated KLH |
recognizes nitrated protein; MW for nitrated-BSA = 85 kDa |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
|
Mouse
(gel immobilized, PBS, azide, cross-linked to protein G agarose) |
IP |
Non-specific |
immunogen = nitrated KLH |
- |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG2b,κ |
Mouse
(liquid, Tris, NaCl, merthiolate, glycerol, HRP conj.) |
ELISA, WB |
Non-specific |
immunogen = nitrated KLH |
recognizes nitrated protein |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG2b,κ |
Mouse
(liquid, protein-G purified, Cy5.5 conj.) |
WB |
Various vertebrate |
immunogen = nitrated KLH |
recognizes nitrated protein |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG2b,κ |
Mouse
(liquid, protein-G purified, Alexa Fluor® 488 conj.) |
FC, WB |
Various vertebrate |
immunogen = nitrated KLH |
recognizes nitrotyrosine |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG2b,κ |
Mouse
(liquid, protein-G purified, Alexa Fluor® 555 conj.) |
FC, WB |
Various vertebrate |
immunogen = nitrated KLH |
recognizes nitrated protein |
- |
|
|
Stressgen Enzo Life Sciences |
monoclonal
IgG1 |
Mouse
(liquid, affinity purified, PBS, azide, glycerol) |
IH, WB |
Bovine, Dog, Rabbit, Chicken, Sheep, Pig, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat, Xenopus |
immunogen = Synthetic peptide derived fromsequence near the C-terminusof rat PDI, conjugated to KLH |
detects PDI |
- |
|
|
MBL International |
monoclonal
IgG1 |
Mouse
(liquid IgG, PBS, glycerol, no preservatives) |
IP, WB |
Human, Mouse |
immunogen = human recombinant VDUP-1/Txnip |
VDUP-1 (50 kDa) |
- |
|
|
MBL International |
monoclonal
IgG2b |
Mouse
(liquid IgG, PBS, glycerol, no preservatives) |
IP, WB |
Human, Mouse |
immunogen = human recombinant VDUP-1/Txnip |
VDUP-1 (50 kDa) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, affinity purified, PBS, azide, gelatin) |
ELISA, IF, WB |
Human, Mouse, Rat |
immunogen = peptide mapping near the N-terminus of Sulfiredoxin of human origin |
Sulfiredoxin |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, affinity purified, PBS, azide, gelatin) |
ELISA, IF, WB |
Human, Mouse, Rat |
immunogen = a peptide mapping within an internal region of Sulfiredoxin of human origin |
Sulfiredoxin, 13 kDa |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, glycerol, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide, human |
TXNRD2 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, no preservative, protein-A purified) |
WB |
Human, Mouse, Rat |
immunogen = recombinant full length protein, human |
TXNRD2, 56 kDa |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide, human, C terminal |
TXNRD2, 56 kDa |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, ready to use) |
IH |
Human, Rat |
immunogen = synthetic peptide, human, C terminal |
TXNRD2 |
- |
|
|
Abcam |
monoclonal
IgG1,κ |
Mouse
(liquid, PBS, glycerol, no preservative, protein-G purified) |
ELISA, IP, WB |
Human |
immunogen = recombinant full length protein, human |
TXNRD1 |
- |
|
|
Abcam |
monoclonal
IgG1,κ |
Mouse
(liquid, PBS, glycerol, no preservative, protein-G purified) |
ELISA, IP |
Human |
immunogen = recombinant full length protein, human |
TXNRD1 |
- |
|
|
Zymed/ Invitrogen |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
IP, WB |
Bovine, Human |
immunogen = synthetic peptide derived from the C-terminal region of human and rat VDUP-1/Txnip |
VDUP-1 (50 kDa) |
- |
|
|
Zymed/ Invitrogen |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
WB |
Bovine, Human |
immunogen = synthetic peptide derived from the N-terminal region of human VDUP-1/Txnip |
VDUP-1 (50 kDa), N-terminal region |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatinAlso available as agarose, sc-20146AC) |
IF, IP, WB |
Human, Mouse, Rat |
immunogen = amino acids 1-105,representing full length Trx of human origin |
recognizes 12 kDaTrx1 (Thioredoxin) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, no preservative, affinity purified) |
IC, IF, WB |
Human, Mouse, Rat |
immunogen = recombinant full length peptide, human |
TXNRD1 |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, Tris, BSA, EDTA, azide) |
IC, IH, IP, WB |
Non-specific |
immunogen = nitrated KLH |
reacts with proteins which are nitrated |
- |
|
|
OXIS International, Inc. |
monoclonal
IgG1 |
Mouse
(TBS, BSA, lyophilized) |
IH, WB |
Various species |
immunogen = 4-HNE-modified keyhole limpet hemocyanine |
4HNE; shows almost negligible reactivity With proteins that were treated with other aldehydes such as: 2-nonenal, 2-hexenal, 1-hexenal, 4-hydroxy-2-hexenal, formaldehyde or glutaraldehyde |
- |
|
|
OXIS International, Inc. |
monoclonal
IgG1 |
Mouse
(TBS, BSA, lyophilized) |
IH, WB |
Various species |
immunogen = 4-HNE-modified keyhole limpet hemocyanine |
4HNE; shows almost negligible reactivity With proteins that were treated with other aldehydes such as: 2-nonenal, 2-hexenal, 1-hexenal, 4-hydroxy-2-hexenal, formaldehyde or glutaraldehyde |
- |
|
|
OXIS International, Inc. |
kit
|
Unknown Host
(Colorimetric assay; for tissue homogenates, or cell lysates) |
- |
Various species |
based on the reaction of a chromogenic reagent, N-methyl-2 phenylindole (R1), with MDA and 4-hydroxyalkenals |
The LPO-586 method is designed to assay either MDA alone (in hydrochloric acid ) or MDA in combination with 4-hydroxyalkenals (in methanesulfonic acid); minimum interference from other aldehydes |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Goat
(liquid, antisera, azide) |
ELISA, WB |
Non-specific |
immunogen = 4- hydroxynonenal conjugate |
specific for 4-HNE |
- |
|
|
Abcam |
polyclonal
IgG |
Mouse
(liquid, whole serum, glycerol, no preservative) |
WB |
Rat |
immunogen = recombinant fusion protein corresp. to aa. 471-570 of Rat NOX2/gp91phox |
recognizes NOX2/gp91phox, ~65 kDa |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, BSA, PBS, azide) |
WB |
Human |
immunogen = synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human NOX2/gp91phox |
recognizes NOX2/gp91phox, ~65 kDa |
- |
|
|
Novus Biologicals |
monoclonal
IgG2a |
Mouse
(liquid, ascites, PBS, glycerol, azide) |
IH, IP, WB |
Dog, Human, Mouse, Rat |
immunogen = 3-(4-hydroxy-3-nitrophenyl acetamido) propionic acid conjugated to BSA |
recognizes 3-nitrotyrosine moieties |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(whole rabbit antisera, no preservative) |
IC, IH, IP, WB |
Cow, Human, Monkey, Mouse, Rat |
immunogen = purified full-length liver protein |
detects Protein disulfide isomerase (PDI) protein |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, antisera) |
WB |
Non-specific |
immunogen = 4- hydroxynonenal conjugate |
detection of 4-Hydroxynonenal |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, TBS, no preservative) |
IH, WB |
Non-specific |
immunogen = 4- hydroxynonenal conjugate |
higher affinity for the 4-Hydroxy-2-Noneal (4-HNE) histidine adduct than the 4-HNE lysine or 4-HNE cysteine adducts. Shows almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal, formaldehyde or glutaraldehyde. |
- |
|
|
Abcam |
polyclonal
IgG |
Goat
(liquid, PBS, azide, NaCl, affinity purified) |
ELISA, WB |
Non-specific |
immunogen = 4- hydroxynonenal conj. to KLH |
specifically binds to HNE modified proteins |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, NaCl, EDTA, affinity purified) |
ELISA |
Non-specific |
immunogen = 4- hydroxynonenal conj. to BC |
specifically binds to HNE modified proteins |
- |
|
|
Abcam |
polyclonal
IgG |
Goat
(liquid, PBS, azide, NaCl, EDTA, thimerosal, affinity purified, conj. HRP) |
ELISA, WB |
Non-specific |
immunogen = 4- hydroxynonenal conj. to KLH |
specifically binds to HNE modified proteins |
- |
|
|
Abcam |
polyclonal
IgG |
Goat
(liquid, PBS, azide, NaCl, EDTA, azide, affinity purified, conj. biotin) |
ELISA, WB |
Non-specific |
immunogen = 4- hydroxynonenal conj. to KLH |
specifically binds to HNE modified LDL, and other HNE modified proteins |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum, azide) |
ELISA |
Non-specific |
immunogen = 4- hydroxynonenal conj. to BSA |
recognises 4 Hydroxynonenal. Reactive with 4 HNE conjugated BSA; minimal cross reactivity with other aldehyde BSA conjugates |
- |