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Antibodies

299 Results

Name Source Clonality &
Isotype
Host &
Formulation
Method Reactivity Immunogen/
Epitope
Specificity Availability
GSK-3α & GSK-3β From manufacturers (99)
Rockland polyclonal
Rabbit
(liquid, sterile filtered, K-phosphate saline, azide, affinity pure)
ELISA, IF, IP, WB Fish (various), Dog, Chicken, Frog, Zebra, Human, Rat Immunogen = synthetic peptide (R P R T T s F A E) corresponding to aa 4-12 of human GSK3β GSK3β (phosphorylated S9) Very weak reactivity against GSK3α. -
Stressgen Enzo Life Sciences polyclonal
Ig
Rabbit
(liquid, PBS, glycerol, BSA, azide, affinity purified)
ELISA, WB Human, Hamster, Mouse, Rat Immunogen = synthetic peptide containing human GSK-3β phosphorylated Ser9 GSK-3β phosphorylated at Ser9 -
US Biological polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
ELISA, WB Human, Hamster, Mouse, Rat Immunogen = synthetic phospho-peptide containing serine 9 of human GSK3β GSK3β phosphorylated -
Chemicon and Upstate are Millipore polyclonal
Rabbit
(liquid, PBS, gl;ycerol, azide, affinity purified)
IC, IP, WB Bovine, Human, Mouse, Rat Immunogen = synthetic peptide corresponding to aa 335-349 of rat GSK-3β conj to KLH GSK-3β (47kDa) -
LifeSpan Biosciences, Inc. monoclonal
Rabbit
(supernatant)
IC, IH, IP, WB Human immunogen = synthetic peptide corresp. to residues near the N-terminal residues of human GSK3 GSK3α -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(protein A purified)
ELISA, IP, WB Xenopus immunogen = recombinant Xenopus laevis protein recognizes two isoforms of glycogen synthase kinase, GSK-3α, a 51kD protein and GSK-3β, a 47kD protein -
LifeSpan Biosciences, Inc. polyclonal
Chicken
(DEAE-column chromatography)
ELISA, IH, WB Human, Rat immunogen = synthetic peptide GSK-3A peptide (aa89-100) GSK-3α -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(protein A column)
ELISA, IH, WB Zebra fish, Human, Mouse, Rat immunogen = synthetic phosphopeptide derived from the sequence of human GSK-3α (KLH coupled) detects endogenous levels of GSK-3 only when phosphorylated at Ser21 of GSK-3α or Ser9 of GSK-3β -
LifeSpan Biosciences, Inc. monoclonal
Mouse
ELISA, WB Human immunogen = synthetic peptide corresp. to a portion of GSK-3 (glycogen synthase kinase-3 ) phosphorylated at Ser21 detects endogenous levels of GSK-3 only when phosphorylated at Ser21 of GSK-3α -
LifeSpan Biosciences, Inc. monoclonal
Mouse
IP, WB Human, Mouse, Rat - GSK-3α -
LifeSpan Biosciences, Inc. monoclonal
Mouse
IP, WB Human, Mouse, Rat - GSK-3α -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(protein G purified)
IH, WB Bovine, Dog, Porcine, Rabbit, Sheep, Human, Monkey, Hamster, Mouse, Rat, Xenopus immunogen = recombinant Xenopus laevis GSK-3beta GSK-3α (~51 kD) and GSK-3β (~47kD) -
LifeSpan Biosciences, Inc. monoclonal
Rabbit
(protein A column)
IP, WB Human, Mouse, Rat immunogen = synthetic peptide (13aa) corresp. to aa471-483 (Q A P D A T P T L T N S S) of rat GSK3α (KLH coupled with glutaraldehyde) GSK3α (~51kDa) -
LifeSpan Biosciences, Inc. monoclonal
Rabbit
(protein A column)
IP, WB Human, Mouse, Rat immunogen = synthetic peptide (13aa) corresp. to aa471-483 (Q A P D A T P T L T N S S) of rat GSK3α (KLH coupled with glutaraldehyde) GSK3α (~51kDa) -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(protein A column)
IP, WB Human, Mouse, Rat immunogen = synthetic peptide (KLH-coupled) derived from the sequence of human GSK-3α detects endogenous levels of GSK-3α; not recombinant GSK-3β -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(protein A column)
IP, WB Human, Rat immunogen = synthetic peptide (13aa, Q A P D A T P T L T N S S) corresp. to aa471-483 of rat GSK-3α (KLH coupled) GSK-3α (~51kDa) -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(unpurified serum)
ELISA, WB Human, Mouse, Rat immunogen = synthetic phospho-peptide derived from a region of human GSK-3 / that contains tyrosine 279/216 GSK3α phosphorylated at Tyr279 and GSK3β phosphorylated at Tyr216 -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(protein G purified)
IC, WB Human, Mouse, Rat, S. pombe, Drosophila, Dictyostelium immunogen = synthetic peptide corresp. to aa203-219 (18aa, C K Q L L H G E P N V S Y I C S R Y) derived from a region of the catalytic domain (between subdomain VII and VIII) of the Drosophila GSK-3/ shaggy enzyme (ovalbumin coupled) GSK-3 -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(DEAE-column chromatography)
ELISA, IH Chicken, Human, Monkey, Mouse, Rat immunogen = synthetic peptide derived from C-terminal of human GSK-3 GSK-3 -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(DEAE-column chromatography)
ELISA, WB Human, Mouse, Rat immunogen = synthetic phospho-from human GSK3α/β containing phosphorylated tyrosine 216/279 GSK3α phosphorylated at Tyr279 (~51kD) and GSK3β phosphorylated at Tyr216 (~47kD) -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(DEAE-column chromatography)
ELISA Human immunogen = synthetic peptide, R A R T S S F A E P C, corresp. to the aa16-25 of human GSK3α GSK3α both phosphorylated and non-phosphorylated forms -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(DEAE-column chromatography)
ELISA Human immunogen = synthetic peptide, R A R T S pS F A E P C, corresp. to the aa16-25 of human GSK3α GSK3α, pS21 -
LifeSpan Biosciences, Inc. monoclonal
Mouse
WB Human, Mouse, Rat - GSK-3α -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(conj. HRP)
WB Human, Mouse, Rat - GSK-3α -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(conj. HRP)
WB Human, Mouse, Rat - GSK-3α -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(conj. HRP)
WB Human, Mouse, Rat - GSK-3α -
LifeSpan Biosciences, Inc. polyclonal
Chicken
(DEAE-column chromatography)
ELISA Human, Mouse, Rat immunogen = synthetic peptide GSK-3A peptide (aa89-100) GSK-3α -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(protein A purified)
WB Human, Rat immunogen = synthetic peptide C R A R T S [pS] F A E P G, corresp. to aa 16-26 of the human GSK3α KLH conj. phosphorylated GSK-3α (~51kD) and phosphorylated recombinant GSK-3β; not active dephosphorylated GSK-3α or GSK-3β -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(DEAE-column chromatography)
ELISA, WB Zebra fish, Human, Monkey, Mouse, Rat immunogen = synthetic phospho-peptide corresp. to residues surrounding Ser21 of human GSK-3α, KLH coupled detects endogenous levels of GSK-3α only when phosphorylated at Ser21 of GSK-3α -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(protein A purified)
WB Human, Rat immunogen = synthetic peptide containing R T S[pS] F A in which pS is phospho-serine corresp. to residue 21 of human GSK3a, KLH conj. detects endogenous levels of GSK-3α only when phosphorylated at Ser21 of GSK-3α -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(DEAE-column chromatography)
ELISA, IF, IH, IP, WB Human immunogen = synthetic peptide, R P R T T S F A E, corresp. to the aa 4-12 of human GSK-3β phosphorylated GSK-3β pS9; minimal reactivity against non-phosphorylated GSK3β -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(protein A purified)
ELISA, IC, IF, IH, WB Zebra fish, Human, Monkey, Mouse, Rat immunogen = synthetic phospho-peptide derived from the seq. of human GSK-3β, KLH coupled detects endogenous levels of GSK-3β only when phosphorylated at Ser9; not the phosphorylated form of GSK3α -
LifeSpan Biosciences, Inc. monoclonal
Rabbit
(supernatant)
FC, IC, IH, IP, WB Human immunogen = synthetic peptide corresp. to residues in the C-terminus of human GSK-3β GSK-3β -
LifeSpan Biosciences, Inc. polyclonal
Chicken
(DEAE-column chromatography)
ELISA, IC, IH, WB Human, Mouse, Rat immunogen = GSK-3β (aa21-36) synthetic peptide GSK-3β -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(unpurified serum)
ELISA, IH, IP, WB Human, Rat, Xenopus immunogen = recombinant Xenopus laevis GSK-3β protein GSK-3β -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(protein A column)
ELISA, IH, IP, WB Human, Rat, Xenopus immunogen = recombinant Xenopus laevis GSK-3β protein GSK-3β -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(protein G column)
ELISA, IH, IP, WB Human, Rat, Xenopus immunogen = recombinant GSK-3 GSK-3β -
LifeSpan Biosciences, Inc. monoclonal
Rabbit
(supernatant)
IC, IH, IP, WB Human immunogen = synthetic peptide corresp. to residues near the N-terminus of the human glycogen synthase GSK-3β -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(protein G column)
ELISA, IH, WB Human, Rat, Xenopus immunogen = recombinant GSK-3β GSK-3β -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(DEAE-column chromatography)
IP, WB Bovine, Human, Mouse, Rat immunogen = 15 residue synthetic peptide (C A H S F F D E L R D P N V K) GSK-3β rhGSK-3β (~47kDa) -
LifeSpan Biosciences, Inc. monoclonal
Mouse
ELISA, IP, WB Human - GSK-3β -
LifeSpan Biosciences, Inc. monoclonal
Mouse
ELISA, IP, WB Human - GSK-3β -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(DEAE-column chromatography)
IC, IP, WB Bovine, Human, Mouse, Rat immunogen = Rat GSK3β synthetic peptide (aa335-349), conjugated to KLH GSK-3β -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(protein G purified)
ELISA, IP, WB Human, Rat, Xenopus immunogen = recombinant Xenopus laevis GSK-3β GSK-3β -
LifeSpan Biosciences, Inc. monoclonal
Rabbit
(supernatant)
IC, IH, WB Human immunogen = synthetic peptide corresp. to residues in the C-term of human GSK-3 GSK-3β -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(DEAE-column chromatography)
ELISA, WB Human, Mouse, Rat immunogen = synthetic peptide corresp. to aa6-12 (R T T [pS] F A E) of human GSK3b, KLH coupled phosphorylated GSK-3β (pSer9) at 46kD -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(Protein G purified)
ELISA, WB Rat, Xenopus immunogen = recombinant GSK3β from Xenopus laevis GSK-3β -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(unpurified serum)
ELISA, IF, IH, IP, WB Human, Rat immunogen = synthetic phospho-peptide derived from a region of human GSK-3b that contains Serine 9 GSK-3β pSer9 -
LifeSpan Biosciences, Inc. monoclonal
Mouse
ELISA, WB Human - GSK-3β -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(HRP conj.)
ELISA, WB Human - GSK-3β -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(HRP conj.)
ELISA, WB Human immunogen = synthetic peptide corresp. to a portion of N-terminal GSK-3 GSK-3β -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(DEAE-column chromatography)
ELISA, WB Human, Hamster, Mouse, Rat immunogen = synthetic peptide human GSK3β containing phosphorylated Ser 9 phosphorylated GSK-3β (pSer9) at 46kD; detects a unknown protein at ~110kD -
LifeSpan Biosciences, Inc. monoclonal
Mouse
WB Human, Mouse, Rat - GSK-3β -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(protein A purified)
WB Bovine, Human, Mouse, Rat immunogen = synthetic peptide from kinase subdomain XI region of the rat GSK3β protein GSK-3β -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(protein A purified)
WB Bovine, Human, Mouse, Rat immunogen = synthetic peptide from kinase subdomain XI region of the rat GSK3β protein GSK-3β -
LifeSpan Biosciences, Inc. polyclonal
Chicken
(DEAE-column chromatography)
ELISA Human, Mouse, Rat immunogen = synthetic peptide corresp. to aa21-36 GSK-3β -
Upstate and Chemicon are Millipore polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified)
WB Human, Rat Immunogen = peptide corresp. to amino acid region encompassing the human and rat phospho-GSK-3β (Ser9) GSK3β phosphorylated Ser9, (47kDa) -
Upstate and Chemicon are Millipore monoclonal
IgG1κ
Mouse
(PBS, PEG, sucrose, azide, glycerol, purified)
ELISA, WB Human, Mouse, Rat Immunogen = synthetic peptide corresponding to aa 6-12 of human GSK-3β GSK-3β phosphorylated Ser9 (46kDa) -
Upstate and Chemicon are Millipore monoclonal
IgG1
Mouse
(liquid, Tween, PBS, BSA,, azide, Protein G purified, Alexa Fluor® 555 conj.)
FC, IC, IF, WB Various vertebrate Immunogen = synthetic peptide corresp. to aa 203-219 derived from the region of the catalytic domain of Drosophila GSK/shaggy enzyme, coupled to ovalbumin GSK3α (51 kDa ) or GSKβ (46 kDa ) -
Upstate and Chemicon are Millipore monoclonal
IgG1κ
Mouse
(frozen liquid, tris-glycine, NaCl, azide, Protein G purified)
IC, WB Human, Mouse, Rat, S. pombe, Dictyostelium Immunogen = synthetic peptide (18 residues)corresp. to aa 203-219 derived from a region of the catalytic domain of Drosophila GSK-3/shaggy enzyme, coupled to ovalbumin. GSK-3 -
Upstate and Chemicon are Millipore monoclonal
IgG1κ
Mouse
(frozen solution of Tris-glycine, NaCl, azide)
WB Human, Mouse, Rat, S. pombe, Dictyostelium Immunogen = synthetic peptide corresponding to aa 203-219 derived from a region of the catalytic domain of the Dropophila GSK/shaggy enzyme coupled to ovalbumin GSK-3 phosphorylated at Tyr279/216 -
Upstate and Chemicon are Millipore monoclonal
IgG
Rabbit
(liquid, tris-glycine, NaCl, glycerol, azide, Protein A purified)
IP, WB Human, Mouse, Rat Immunogen = synthetic peptide corresp. to aa 471-483 of rat GSK-3α: GSK-3α (51kDa) -
Upstate and Chemicon are Millipore polyclonal
IgG
Rabbit
(liquid, tris-glycine, naCl, azide, glycerol, Protein A purified)
IP, WB Human, Rat Immunogen - synthetic peptide corresp. to aa 471-483 of rat GSK3α GSK-3α -
Upstate and Chemicon are Millipore monoclonal
IgG1κ
Mouse
(liquid tris-glycine, NaCl, azide, glycerol, Protein G purified)
WB Human, Rat Immunogen = synthetic peptide containing ...RTS[pS]FA... in which pS is phosphoserine 21 of human GSK-3α GSK-3α phosphorylated (51kDa). Non-specific proteins may also be detected (66 and 120 kDa) -
Upstate and Chemicon are Millipore polyclonal
IgG
Rabbit
(liquid tris-glycine, NaCl, azide, BSA, glycerol, Protein A purified)
WB Human, Rat Immunogen = synthetic peptide corresponding to aa 16-26 of human phospho-GSK3α (Ser21) with an N-terminal cysteine added for conjugation. Phospho-GSK3α (Ser21, 51kDa) -
Upstate and Chemicon are Millipore polyclonal
IgG
Sheep
(liquid tris-glycine, NaCl, azide, glycerol, Protein G purified)
WB Human, Rat Immunogen = synthetic peptide corresponding to aa 16-26 of human phospho-GSK-3α (Ser21) with an N-terminal cysteine added for conjugation. GSK-3α phosphorylated at Ser21 -
Upstate and Chemicon are Millipore monoclonal
IgG
Rabbit
(liquid, tris-glycine, NaCl, glycerol, azide, Protein A purified)
IP, WB Human, Mouse Immunogen = N-terminal 6His-tagged recombinant full length human GSK3β expressed by baculovirus in Sf21 insect cells, purified GSK3α (51 kDa) and GSK3β (46 kDa) -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, PBS, no preservative)
DB, ELISA Human, Mouse, Rat Immunogen = synthetic peptide derived from the regions of GSK-3α and β that contain tyrosine 216/279 GSK3α and GSK3β -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, affinity purified, phosphate buffer, azide)
ELISA, WB Human immunogen = synthetic peptide R A R T S pS F A E P C, corresp. to aa 16-25 of human GSK3α 52 kDa GSK3α phospho S21 -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, affinity purified, phosphate buffer, azide)
ELISA, WB Human immunogen = synthetic peptide R A R T S S F A E P C, corresp. to aa 16-25 of human GSK3α 52 kDa GSK3α, phospho and non-phospho -
Novus Biologicals polyclonal
IgY
Chicken
(liquid, PBS, no preservative)
ELISA, IH, WB Human immunogen = synthetic peptide corr. to internal sequence (aa 89-100) of human GSK3 alpha GSK3 alpha, cytoplasmic -
Invitrogen Corporation polyclonal
Rabbit
(liquid Ig, PBS, BSA, glycerol, affinity purified, unconjugated, no preservative)
DB, ELISA, WB Human, Mouse, Rat immunogen = synthesized phospho peptide derived from a region of human GSK-3α/β that contains tyrosine 279/216. GSK3α phosphorylated at Tyr279 (~51kD) and GSK3β phosphorylated at Tyr216 (~47kD) -
Sigma Aldrich monoclonal
IgG1
Mouse
(liquid, ascites fluid, azide)
ELISA, IC, WB Rabbit, Human, Mouse, Rat Immunogen = recombinant rabbit GSK-3β GSK-3β. Not GSK-3α -
ECM Biosciences LLC monoclonal
IgG1
Mouse
(liquid, PBS, BSA, glycerol, azide, protein-A purified,)
ELISA, WB Rabbit, Human immunogen = phospho-GSK-3β (Tyr-216) syntheticpeptide (coupled to KLH) corresponding to amino acid residues aroundtyrosine 216 of human GSK-3β This antibody detects 46/50 kDa proteins correspondingto the apparent molecular mass of GSK-3β and GSK-3α -
A B R - Thermo Scientific polyclonal
IgG
Rabbit
(epitope affinity purified IgG, PBS, BSA, azide)
ELISA, IH, IP, WB Human, Mouse immunogen = synthetic peptide corresponding to human GSK3 beta detects GSK3 β -
A B R - Thermo Scientific polyclonal
IgG
Rabbit
(epitope affinity purified IgG, PBS, BSA, azide)
ELISA, IH, IP, WB Human, Mouse immunogen = synthetic phosphopeptide corresp. to residues around the phosphorylation site at Serine 9 of human GSK3 beta detects phospho-GSK3β from human and mouse samples -
A B R - Thermo Scientific monoclonal
IgG2a
Mouse
(protein-A affinity purified, PBS, azide)
ELISA, IH, IP, WB Human, Rat, Xenopus immunogen = purified recombinant Xenopus laevis protein detects GSK3 β -
A B R - Thermo Scientific polyclonal
IgG
Rabbit
(IgG, Dulbeccos’ PBS free of Mg2+ and Ca2+)
ELISA, WB Human, Mouse, Rat immunogen = synthetic phosphopeptide derived from regions of human GSK3α/β that contain tyrosine 279/216 respectively detects an ~51 kDa protein and an ~47 kDa protein from stimulated 3T3-L1 cells representing tyrosine 279 phospho. GSK3alpha and tyrosine 216 phospho. GSK3beta -
Zymed/ Invitrogen monoclonal
Mouse
(liquid, affinity purified, unconjugated)
ELISA, WB Human, Mouse, Rat - recognizes the expressed product of the GSK3B gene -
AnaSpec polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affintiy purified)
IH Chicken, Human, Monkey immunogen = a synthetic peptide from the C-terminus of human GSK-3 protein detects GSK-3 -
Cell Signaling monoclonal
IgG
Rabbit
(liquid, HEPES, BSA, glycerol, NaCl, azide)
IP, WB Human, Monkey, Hamster, Mouse, Rat Immunogen = a synthetic peptide corresponding to the sequence of human GSK-3α protein detects endogenous levels of total GSK-3α protein. The antibody does not cross-react with GSK-3β. -
Cell Signaling monoclonal
IgG
Rabbit
(liquid, HEPES, BSA, glycerol, NaCl)
FC, IF Mouse Immunogen = a synthetic peptide corresponding to the sequence of human GSK-3α protein detects endogenous levels of total GSK-3α protein; does not cross-react with GSK-3β. -
Cell Signaling monoclonal
IgG
Rabbit
(HEPES, NaCl, BSA, glycerol, azide)
IH, WB Human, Monkey, Mouse, Rat Immunogen = synthetic phospho-peptide corresp. to residues around Ser21 of human GSK-3α detects GSK-3α only when phosphorylated at serine 21; does not detect GSK-3β when phosphorylated at Ser9 -
Cell Signaling polyclonal
Rabbit
(HEPES, BSA, glycerol, NaCl, Protein A and affinity purified)
WB Human, Monkey, Mouse, Rat Immunogen = synthetic peptide derived from the sequecne of human GSK-3α detects GSK-3α (total). No cross with recombinant GSK-3β -
Cell Signaling monoclonal
IgG2b
Mouse
(PBS, Protein G purified)
ELISA Human Immunogen = synthetic phospho-peptide corresp. to residues surrounding serine 21 of human GSK-3α detects peptides derived from GSK-3α phosphorylated at serine 21 -
Cell Signaling monoclonal
IgG2b
Mouse
(HEPES, NaCl, BSA, glycerol, azide)
WB Zebra fish, Human, Monkey, Mouse, Rat Immunogen = synthetic phospho-peptide corresponding to residues surrounding Ser21 of human GSK-3α detects GSK-3α only when phosphorylated at serine 21 -
Cell Signaling monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
IH, IP, WB Human, Monkey, Mouse, Rat Immunogen = synthetic peptide derived from the sequence of human GSK-3β recognizes endogenous levels of GSK-3β protein -
Cell Signaling monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol)
IP, WB Human, Monkey, Mouse, Rat Immunogen = a synthetic phospho-peptide(KLH-coupled) corresponding to residues around Ser21 of human GSK-3a preferentially reacts with endogenous levels of GSK-3α when phosphorylated at Ser21 and also with GSK-3β when phosphorylated at Ser9 -
Cell Signaling polyclonal
Rabbit
(protein A, affinity purified, HEPES, BSA, glycerol)
IH, WB Zebra fish, Human, Monkey, Mouse, Rat Immunogen = synthetic phospho-peptide (KLH coupled) derived from the sequence of human GSK-3α reacts with endogenous levels of GSK-3 only when phosphorylated at Ser21 of GSK-3α, Ser9 of GSK-3β. -
Cell Signaling polyclonal
Rabbit
(liquid, affinity purified, HEPES, saline, BSA, glycerol)
WB Human immunogen = a synthetic phosphopeptide corresp. to residues surrounding Thr390 of human GSK-3β recognizes endogenous levels of human GSK-3β protein only when phosphorylated at Thr390 -
Cell Signaling monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
IP, WB Human, Mouse, Rat immunogen = a synthetic phosphopeptide corresponding to residues around Ser9 of human GSK-3β recognizes endogenous levels of GSK-3 only when phosphorylated at Ser9 of GSK-3β -
Cell Signaling polyclonal
Rabbit
(protein A, affinity purified, HEPES, BSA, glycerol)
IC, IH, WB Human, Monkey, Mouse, Rat immunogen = syntheticphospho-peptide (KLH coupled) derived from the sequence of human GSK-3β recognizes endogenous levels of GSK-3 only when phosphorylated at Ser9 of GSK-3β may cross weakly with the phosphorylated form of GSK-3α due to high sequence homology -
Cell Signaling monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
IC, IF, IH, WB Human, Monkey, Mouse, Rat immunogen = a synthetic phosphopeptide corresponding to residues around Ser9 of human GSK-3β recognizes endogenous levels of GSK-3 only when phosphorylated at Ser9 of GSK-3β may cross weakly with the phosphorylated form of GSK-3α due to high sequence homology -
BD Transduction monoclonal
IgG1
Mouse
(aqueous buffered solution, glycerol, BSA, azide)
IF, IH, IP, WB Dog, Chicken, Human, Mouse, Rat Immunogen = rat GSK-3β, aa 1-160 detects GSK3β, 46 kDa -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified;also available as rabbit, sc-11757-R)
IF, IH, WB Fish (various), Zebra, Human, Mouse, Rat, Xenopus Immunogen = short amino acid sequence containing phos-phorylated Ser9 of human GSK-3β Ser9 phosphorylated GSK-3β -
Chemicon and Upstate are Millipore monoclonal
IgG
Rabbit
(liquid, Tris-glycine, saline, gl;ycerol, azide, BSA)
IC, IH, IP, WB Human Immunogen = phospho-specific peptide corresponding to residues surrounding Ser9 of human GSK3 β detects only GSK3 β phosphorylated on Ser9 -
None polyclonal
Rabbit
(PBS, azide, glycerol, affinity purified)
WB Human, Mouse Immunogen = recombinant (partial), C-terminal human GSK3β GSK3β. Cross reactive with GSK3α discontinued
None polyclonal
Rabbit
(liquid PBS, no preservative, affinity purified)
DB, ELISA, WB Human, Mouse, Rat Immunogen = synthetic peptide derived from the regions of GSK-3β that contain tyrosine 216/279 GSK-3β phosphorylated Tyr279/216 (51kDa) Discontinued
None polyclonal
Rabbit
(liquid PBS, no preservative, affinity purified)
DB, ELISA, WB Human, Mouse, Rat Immunogen = sequence corresponding to GSK-3β surrounding serine 9 GSK-3β phosphorylated Ser9 (51kDa) Discontinued