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Antibodies

132 Results

Name Source Clonality &
Isotype
Host &
Formulation
Method Reactivity Immunogen/
Epitope
Specificity Availability
Posttranslational Modifications From labs (2)
Ubi-B1 and Ubi-B2(Fred W. van Leeuwen, Netherlands Institute forBrain Research, Amsterdam, Netherlands)Paper polyclonal
Rabbit
(See also R&D systems antibodies MAB703 andAF703)
IB, IC, IH Human immunogen = synthetic peptides covering YADLRED PDRQDHH PGSGAQ at the C-terminus of Ubiquitin+1 reacts with Ubiquitin+1 and not Ubiquitin -
(Sungmin Song, Department of Life Science, Kwangju Institute of Science and Technology, Kwangju, Korea)PaperReference: Ko, S, 2010 monoclonal
Rat
(liquid, affinity purified)
IC, IH, WB Human immunogen = synthetic peptide of the same amino acid sequence as previously described in van Leeuwen et al., 1998 reacts with Ubiquitin+1 and not Ubiquitin -
Posttranslational Modifications From manufacturers (30)
GeneTex polyclonal
IgG
Mouse
(liquid, antiserum, unpurified, glycerol)
ELISA, WB Human immunogen = PIAS3 (NP_006090, 453 a.a. ~ 551 a.a) partial recombinant protein with GST tag detects PIAS3 -
GeneTex polyclonal
IgG
Rabbit
(liquid, protein-A purified, PBS, sucrose)
IH, WB Human immunogen = synthetic peptide corresponding to a region of human PIAS3 detects PIAS3 -
Sigma Aldrich monoclonal
IgG1
Mouse
(liquid, buffered aqueous solution, PBS, azide)
ELISA, IC, WB Bovine, Dog, Chicken, Human, Mouse immunogen = synthetic peptidecorresp. to amino acids 591-604 of human PIAS3 detects PIAS3 -
Sigma Aldrich monoclonal
IgG2
Mouse
(buffered aqueous solution, purified Ig, PBS)
ELISA, WB Human immunogen = PIAS3 (NP_006090, 453 a.a. ~ 551 a.a) partial recombinant protein with GST tag detects PIAS3 -
R&D Systems polyclonal
IgG
Goat
(IgG fraction, affinity purified, PBS, trehalose, lyophilized)
WB Human immunogen = purified, E. coli-derived, recomb. human PIAS3, aa 1-619 detects endogenous human PIAS3 -
Santa Cruz polyclonal
IgG
Goat
(liquid, affinity purified, PBS, gelatin, azide)
ELISA, IF, WB Human, Mouse, Rat immunogen = peptide mapping at the C-terminus of PIAS 3 of mouse origin detects PIAS3 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, gelatin, azide)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = against amino acids451-619 mapping at the C-terminus of PIAS 3 of human origin detects PIAS3 -
Cell Signaling monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
IC, IF, IP, WB Human, Mouse, Rat immunogen = a synthetic peptide from the amino terminus of human SUMO-1 detects recombinant SUMO-1 and endogenous levels of sumoylated proteins (e.g. SUMO-1-RanGAP at 90 kD); does not detect recombinant SUMO-2 or SUMO-3 -
Cell Signaling monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
IH, IP, WB Human, Mouse, Rat immunogen = a synthetic peptide from the amino terminus of human SUMO-1 detects recombinant SUMO-1 and endogenous levels of sumoylated proteins (e.g. SUMO-1-RanGAP at 90 kD); does not detect recombinant SUMO-2 or SUMO-3 -
Cell Signaling monoclonal
IgG1
Mouse
(liquid, HEPES, saline, BSA, glycerol, azide)
WB Human, Mouse, Rat immunogen = a synthetic peptide corresp. to a sequence within human SUMO-1 that does not correspond to SUMO-2/3 detects recombinant SUMO-1 and endogenous levels of sumoylated proteins (e.g. SUMO-1-RanGAP at 90 kD); does not detect recombinant SUMO-2 or SUMO-3 -
Cell Signaling polyclonal
Rabbit
(liquid, affinity purified, HEPES, saline, BSA, glycerol)
IC, IF, IH, IP, WB Human, Monkey, Mouse, Rat immunogen = a synthetic peptide corresp. to a sequence within human SUMO-1 that does not correspond to SUMO-2/3 detects recombinant SUMO-1 and endogenous levels of sumoylated proteins (e.g. SUMO-1-RanGAP, 90kD) -
Cell Signaling monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
IC, IF, WB Human, Mouse, Rat immunogen = a synthetic peptide from the amino terminus of human SUMO-3 recognizes endogenous levels of SUMO-2/3. It does not cross-react with recombinant SUMO-1 -
AnaSpec polyclonal
Rabbit
(liquid, affinity purified, PBS, BSA, azide)
IH Human immunogen = a recombinant full length human ubiquitin protein detects Ubiquitin -
Sigma Aldrich polyclonal
Rabbit
(serum, PBS, predilute, no preservative, lyophilized)
IH, WB Various species immunogen = bovine red blood cell ubiquitin conj. to KLH ubiquitin -
Santa Cruz monoclonal
IgG2b
Mouse
(liquid, PBS, azide, gelatin)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = amino acids 1-76representing full length Ub of human origin. detects ubiquitin and polyubiquitin -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
ELISA, IP, WB Yeast, Cow, Human, Mouse, Rat immunogen = full length denatured ubiquitin of bovine origin detects ubiquitin, polyubiquitin and ubiquitin conjugated proteins -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = amino acids 1-76representing full length Ub of human origin. detects ubiquitin and polyubiquitin -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatinAlso available as agarose, HRP or FITC conj. and as Alexa Fluor®)
IF, IH, IP, WB Human, Mouse, Rat, Drosophila epitope corr. to aa 1-76 representing full length bovine Ubiquitin detects ubiquitin, poly-ubiquitin and ubiquitinated proteins -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
FC, IF, IH, IP, WB Cow, Human immunogen = Ub from cowerythrocytes conjugated to bovine gammaglobulin detects ubiquitin -
Santa Cruz monoclonal
IgG2a
Mouse
(liquid, azide)
IP, WB Non-specific immunogen = cow erythrocyteubiquitin detects ubiquitin -
Santa Cruz monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
ELISA, IF, IH, IP, WB Human immunogen = full length recombinantUb of human origin detects ubiquitin -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, ascites, azide)
IF, IH, IP, WB Cow, Human, Mouse, C. elegans, Drosophila immunogen = purified ubiquitinof cow origin detects ubiquitin -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, supernatant, IgG, azide)
IF, IH, WB Human, Mouse, Rat immunogen = Ubiquitin from red blood cells of cow origin detects ubiquitin, 8.5 kDa in WB -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, ascites, azide)
IH, WB Human immunogen = purified ubiquitin (bovine RBC) covalently coupled to KLH using glutaraldehyde ubiquitin, paired helical filaments (PHF) of AD, Pick body and Lewy body -
Dako polyclonal
Rabbit
(purified Ig fraction, saline, azide)
ELISA, IC, IEP, WB Baboon, Human, Wistar rat immunogen = Ubiquitin isolated from cow erythrocytes and conj. to chicken gamma globulins with glutaraldehyde periphery of senile plaques and of neurofibrillary tangles, Lewy bodies, Mallory bodies -
R&D Systems monoclonal
IgG2A
Mouse
(IgG fract, protein-G, PBS, filtered, lyophilized)
WB Human immunogen = KLH coupled synthetic peptide CADLRED PDRQDHH PGSGAQ corr. to the C-terminus of Ubiquitin+1 Ubiquitin+1; not Ubiquitin -
None monoclonal
IgG1
Mouse
(purified)
ELISA, WB Human, Various species, Yeast immunogen = denatured bovine ubiquitin recognizes ubiquitin, polyubiquitin, and ubiquitin-conjugated proteins Discontinued
None monoclonal
IgG1
Mouse
(ascites)
WB Human, Various species, Yeast immunogen = denatured bovine ubiquitin recognizes ubiquitin, polyubiquitin, and ubiquitin conjugated proteins Discontinued
None monoclonal
IgG1
Mouse
(ascites, azide)
ELISA, IH, WB Bovine, Human, Chicken, Drosophila, Nematode immunogen = purified KLH-conjugated ubiquitin ubiquitin; insensitive to formalin fixation, neurofibrillary tangles, Lewy bodies of PD and the Pick bodies in Pick's disease Discontinued
- monoclonal
IgM
Mouse
(liquid, ascites)
ELISA, IP, WB Chicken immunogen = a synthetic peptide containing Serine-O-GlcNAc detects O-GlcNAc (ß-O-linked N- acetylglucosamine) and Thr-O-GlcNAc but shows no cross reactivity with peptide determinants or other closely-related carbohydrate antigens Discontinued