|
|
Biogenesis AdB Serotec |
polyclonal
|
Rabbit
(serum, lyophilized) |
RID, WB |
- |
immunogen = purified bovine ubiquitin |
ubiquitin |
- |
|
|
Biotrend |
polyclonal
|
Rabbit
(purified IgG, PBS) |
IH |
Cow, Human, Mouse, Rat |
immunogen = ubiquitin from bovine erythrocytes |
ubiquitin |
- |
|
|
Biotrend |
polyclonal
|
Rabbit
(serum, lyophilized) |
RID, WB |
- |
immunogen = purified bovine ubiquitin |
ubiquitin |
- |
|
|
Biotrend |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
IH, EM, WB |
Human |
immunogen = purified ubiquitin covalently coupled to KLH using glutaraldehyde |
Ubiquitin |
- |
|
|
Kamiya |
monoclonal
IgM |
Mouse
(liquid, ascites, azide) |
IH, WB |
Human |
immunogen = recomb. human ubiquitin |
ubiquitin |
- |
|
|
R&D Systems |
polyclonal
IgG |
Rabbit
(affinity purified, filtered, PBS, trehalose, lyophilized) |
IH, WB |
Human |
immunogen = KLH coupled synthetic peptide CADLRED PDRQDHH PGSGAQ corr. to the C-terminus of Ubiquitin+1 |
rhUbiquitin+1 |
- |
|
|
R&D Systems |
monoclonal
IgG2B |
Mouse
(IgG fract, protein-G, PBS, filtered, lyophilized) |
IH, WB |
Human |
immunogen = recombinant Ubiquitin+1 containing a His10 tag at the amino terminal |
Ubiquitin and Ubiquitin+1 |
- |
|
|
RDI /Fitzgerald |
monoclonal
IgG1 |
Mouse
|
IH, FC, WB |
Human, Mouse, Rat, Various species |
aa 1-46 of the amino terminus of ubiquitinof bovine origin |
ubiquitin, poly-ubiquitin and ubiquitinated proteins |
- |
|
|
Sigma Aldrich |
monoclonal
IgG1 |
Mouse
(liquid, supernate, purified, PBS, azide) |
WB, ELISA |
Human, Mouse, Rabbit |
immunogen = human and mouse Ubiquitin Activating Enzyme |
E1 (Ubiquitin Activating Enzyme) |
- |
|
|
Sigma Aldrich |
monoclonal
IgG2a |
Mouse
(liquid, ascites, azide) |
WB, ELISA |
Human, Mouse, Rat, Bovine |
immunogen = purified bovine ubiquitin conj. to KLH |
ubiquitin |
- |
|
|
Stressgen Enzo Life Sciences |
polyclonal
|
Rabbit
(whole serum) |
IH, IP, WB |
Sheep, Human, Monkey, Hamster, Mouse, Rat, Drosophila, Fish (various), Bovine, Dog, Rabbit, Chicken, Frog, E. Coli, Yeast |
immunogen = ubiquitin purified from bovine red blood cells, conj. to KLH |
free ubiquitin and ubiquinated proteins; will immuno- precipitate BSA-conjugated ubiquitin |
- |
|
|
Stressgen Enzo Life Sciences |
monoclonal
IgG1 |
Mouse
(protein-A, Ig, PBS, glycerol) |
IH, WB |
- |
immunogen = crude poly ubiquitin lysozyme |
ubiquitinated proteins; not free ubiquitin |
- |
|
|
Zymed/ Invitrogen |
monoclonal
IgG1κ |
Mouse
(liquid, PBS, azide) |
IF, IH, IP, WB, ELISA |
Human, Mouse, C. elegans, Drosophila, Bovine, Chicken |
immunogen = purified bovine ubiquitin conj. to carrier protein |
ubiquitin, both conjugated and unconjugated; reacts with single chain 8.5 kDa protein |
- |
|
|
R&D Systems |
monoclonal
IgG2B |
Mouse
(supernatant, IgG fract, protein-G, PBS, filtered, trehalose, lyophilized) |
WB, ELISA |
Human |
immunogen = recomb. human Ubiquitin+1 |
Ubiquitin+1; not Ubiquitin |
- |
|
|
R&D Systems |
monoclonal
IgG2B |
Mouse
(supernatant, IgG fract, protein-G, Biotin conj, filtered, PBS, BSA, lyophilized) |
WB, ELISA |
Human |
immunogen = purified, E.Coli derived, recomb. human Ubiquitin+1(epitope in the C-terminal segment unique to Ubiquitin+1) |
Ubiquitin+1; not Ubiquitin |
- |
|
|
Covance Research Products, Inc (now BioLegend) |
polyclonal
IgG1 |
Rabbit
(serum) |
IF, WB |
Human |
immunogen = glutaraldehyde cross linked ubiquitin |
recognizes ubiquitin |
- |
|
|
Biomeda |
kit
|
Rabbit
(Tris, carriers, azide, prediluted -staining kit) |
IH |
Human, Mouse, Rat, Bovine, Equine |
immunogen = ubiquitin purified from bovine erythrocytes and conjugated to phycoerythrin-R |
8.5kDa ubiquitin |
- |
|
|
Biomeda |
polyclonal
|
Rabbit
(Tris, carriers, azide, concentrated) |
IH |
Human, Mouse, Rat, Bovine, Equine |
immunogen = ubiquitin purified from bovine erythrocytes and conjugated to phycoerythrin-R |
8.5kDa ubiquitin; nuclear, cytoplasmic and cell membrane |
- |
|
|
Biomeda |
polyclonal
|
Rabbit
(Tris, carriers, azide, buffered, prediluted) |
IH |
Human, Mouse, Rat, Bovine, Equine |
immunogen = ubiquitin purified from bovine erythrocytes and conjugated to phycoerythrin-R |
8.5kDa ubiquitin |
- |
|
|
Biogenesis AdB Serotec |
polyclonal
|
Rabbit
(purified IgG, PBS, stabilizers) |
IH |
Cow, Human, Mouse, Rat |
immunogen = ubiquitin from bovine erythrocytes |
ubiquitin |
- |
|
|
Fitzgerald |
polyclonal
|
Rabbit
(serum, azide) |
IH, WB |
- |
immunogen = bovine ubiquitin |
ubiquitin, especially Lewy bodies in Parkinson disease. |
- |
|
|
RDI /Fitzgerald |
monoclonal
IgG1 |
Mouse
(ascites, affinity purified, azide) |
FC, WB |
Human, Bovine |
immunogen = purified bovine erythrocyte ubiquitin |
human erythrocyte ubiquitin and bovine erythrocyte ubiquitin. Detect a ubiquitin band migrated to MW of 5.5 Kda bySDS-PAGE |
- |
|
|
RDI /Fitzgerald |
monoclonal
IgG1 |
Mouse
(liquid, ascites, affinity purified, azide) |
IH, FC, WB |
Human, Bovine |
immunogen = purified bovine erythrocyte ubiquitin |
human erythrocyte ubiquitin and bovine erythrocyte ubiquitin. Detect a ubiquitin band migrated to MW of 5.5 Kda bySDS-PAGE |
- |
|
|
RDI /Fitzgerald |
monoclonal
IgG1 |
Mouse
(supernate, azide, lyophilized) |
IH, FC, WB |
- |
immunogen = ubiquitin conjugated with glutaraldehyde cross linked to KLH |
reacts to brain sections (tangles) from AD patients. Cytoplasmic filamentous inclusions and dot-like immuno reactivity |
- |
|
|
RDI /Fitzgerald |
polyclonal
|
Rabbit
(serum, azide, lyophilized) |
IH, WB |
Cow, Human, Mouse, Rat |
immunogen = ubiquitin conjugated with glutaraldehyde cross linked to KLH |
reacts to brain sections (tangles) from AD patients. Cytoplasmic filamentous inclusions and dot-like immuno reactivity |
- |
|
|
RDI /Fitzgerald |
polyclonal
IgG |
Goat
|
IH, WB |
Human, Mouse, Rat |
(aa 2-20 of the carboxy terminus of ubiquitin of human origin) |
ubiquitin, neurofibrillary tangles in Alzheimer disease, Lewy bodies |
- |
|
|
Stressgen Enzo Life Sciences |
monoclonal
IgG |
Mouse
(Ig, protein-G, PBS, PMSF) |
WB, ELISA |
Sheep, Human, Hamster, Mouse, Rat, Drosophila, Bovine, Dog, Rabbit, Chicken, Yeast, Guinea Pig |
immunogen = natural source bovine ubiquitin |
both mono and poly ubiquinated proteins |
- |
|
|
EnCor Biotechnology, Inc, |
monoclonal
IgG1κ |
Mouse
(ascites, purified, BSA, KLH) |
IF, IH, WB |
Human, Drosophila, Bovine, Chicken, C. elegans |
purified ubiquitin conj. with glutaraldehyde to keyhole limpet hemocyanin |
Neurofibrillary tangles strongly and specifically; also recognizes ubiquitinated inclusion bodiess such as lewey and pick bodies |
- |
|
|
RDI /Fitzgerald |
polyclonal
|
Rabbit
(serum, prediluted, lyophilized) |
IH, WB |
Human |
immunogen = ubiquitin from bovine red blood cells conjugated to KLH |
detects neurofibrillary tangles in Alzheimer's disease, Lewy bodies in Lewy Body disease; also dot-like structures in the white matter of normal aging brain |
- |
|
|
Signet Covance |
monoclonal
IgG1κ |
Mouse
(ascites) |
IH, IP, WB, ELISA |
- |
|
residues 50-65 of ubiquitin in PHF, not free ubiquitin. Highly reactive on formalin fixed tissues. |
- |
|
|
Signet Covance |
monoclonal
IgG1κ |
Mouse
(ascites) |
IH, IP, WB, ELISA |
- |
|
residues 64-76 of ubiquitin and ubiquitin in PHF. Formalin fixation eliminates reactivity. |
- |
|
|
Stressgen Enzo Life Sciences |
monoclonal
IgG1 |
Mouse
(protein-A, PBS, no preserves, lyophilized) |
IH, WB |
Human, Bovine |
immunogen = purified bovine erythrocyte ubiquitin |
~10 kDa ubiquitin; clone recognizes a different epitope than product SPA-202 |
- |
|
|
Stressgen Enzo Life Sciences |
monoclonal
IgG1 |
Mouse
(protein-A, PBS, no preserves, lyophilized) |
WB |
Human, Bovine |
immunogen = purified bovine erythrocyte ubiquitin |
~10 kDa ubiquitin; clone recognizes a different epitope than product SPA-201 |
- |
|
|
Zymed/ Invitrogen |
monoclonal
IgG1κ |
Mouse
(liquid, purified ascites, PBS, BSA, azide, predilute) |
IH |
Human |
immunogen = bovine ubiquitin |
ubiquitin; reacts with single chain 8.5 kDa protein |
- |
|
|
Biogenesis AdB Serotec |
polyclonal
|
Sheep
(neat serum, filtered, azide) |
ELISA, IH, WB |
Various species |
immunogen = ubiquitin from bovine RBCs |
Ubiquitin |
- |
|
|
Biotrend |
polyclonal
|
Sheep
(neat serum, filtered, azide) |
ELISA, IH, WB |
Various species |
immunogen = ubiquitin from bovine RBCs |
Ubiquitin |
- |
|
|
BD Pharmingen |
monoclonal
IgG1 |
Mouse
(ascites, PBS, azide) |
WB |
Human |
immunogen = bovine erythrocyte ubiquitin was coupled to ovalbumin with glutaraldehyde |
recognizes human ubiquitinated proteins |
- |
|
|
MBL International |
monoclonal
IgG1 |
Mouse
(affinity purified, lyophilized, PBS, no preserves) |
IH, WB |
Bovine, Human, Mouse |
immunogen = purified bovine erythrocyte ubiquitin |
reacts with erythrocyte ubiquitin |
- |
|
|
MBL International |
monoclonal
IgG1 |
Mouse
(affinity purified, lyophilized, PBS, no preserves) |
WB |
Bovine, Human, Mouse |
immunogen = purified bovine erythrocyte ubiquitin |
reacts with erythrocyte ubiquitin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum) |
ELISA, IF, IH, IP, WB |
Chicken, Cow, Human, C. elegans, Drosophila |
immunogen = full length native protein purified and conjugated to KLH by a Glutaraldehyde linker |
recognizes ubiquitinated inclusion bodies |
- |
|
|
Abcam |
monoclonal
IgG1,κ |
Mouse
(supernate, no preservative, lyophilized) |
ELISA, IH, WB |
- |
|
reacts specifically with 8.5 kDa protein and ubiquitinated proteins |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, protein-A purified) |
IF, IH |
Cow, Human, Mouse, Rat |
immunogen = ubiquitin isolated from cow erythrocytes and conjugated to chicken gammaglobulins |
ubiquitin; also stains with the periphery of senile plaques and neurofibrillay tangles in Alzheimer disease |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum, PBS, lactose, no preservative) |
IH, WB |
Cow, S. cerevisiae |
immunogen = bovine red blood cell ubiquitin conjugated to KLH |
ubiquitin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum, tris, carriers, azide) |
ELISA, IH, IP, WB |
Cow, Horse, Human, Mouse, Rat |
immunogen = ubiquitin purified from bovine erythrocytes and conjugated to phycoerythrin-R |
reacts with physiologically occurring conjugates of ubiquitin and intracellular proteins; specifically recognizes ubiquitinated cytoplasmic inclusion bodies |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, IgG fraction, PBS, azide) |
ELISA, FC, IH, WB |
Cow, Human |
immunogen = ubiquitin from bovine erythrocytes conjugated to bovine gamma globulin |
ubiquitin |
- |
|
|
Abcam |
polyclonal
IgG |
Sheep
(liquid, whole antiserum) |
DB, IH, WB |
Rabbit, Sheep, Human |
immunogen = Ubiquitin from Sigma (Product number U6253) that was coupled to chicken gammaglobulin |
both free ubiquitin and ubiquitin protein conjugates; labels tangles and Lewy bodies |
- |
|
|
Abcam |
monoclonal
IgG1,κ |
Mouse
(liquid, ascites, azide) |
IH, IP, WB |
Chicken, Cow, Human, Mouse, C. elegans, Arabidopsis thaliana, Drosophila |
immunogen = full length native protein purified and conjugated to KLH by a Glutaraldehyde linker |
recognizes polyubiquitin chains much more strongly than mono ubiquitinated molecules or free ubiquitin; specifically recognizes ubiquitinated cytoplasmic inclusion bodies |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole serum, no preservative) |
IH, IP, WB |
Various species |
immunogen = full length native protein, purified from bovine red blood cells |
free ubiquitin as well as ubiquinated proteins |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, PBS, BSA, azide) |
IH |
Human |
immunogen = recomb. full length human protein |
ubiquitin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, protein-G purified, PBS, azide) |
ELISA, IH, WB |
Human |
immunogen = KLH-conjugated synthetic peptide selected within aa 1-100 of human Ubiquitin |
ubiquitin, aminoterminal end |
- |
|
|
Abcam |
monoclonal
IgG |
Rabbit
(liquid, IgG fraction, glycerol, BSA, Tris, azide) |
IH, WB |
Human, Mouse, Rat |
immunogen = KLH-synthetic peptide corrsp. to residues in the C-terminal of human Ubiquitin |
ubiquitin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum, green food dye, azide, predilute) |
IH |
Cow, Horse, Human, Mouse, Rat |
immunogen = ubiquitin purified from bovine erythrocytes and conjugated to phycoerythrin-R |
ubiquitin; periphery of senile plaques, neurofibrillary tangles in AD brain, Lewy bodies in Parkinson's; mallory bodies in alcoholic liver disease |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, KPO4-saline, azide, purified) |
ELISA, WB |
Yeast |
immunogen = ubiquitin coupled to rabbit IgG |
ubiquitin |
- |
|
|
Abcam |
polyclonal
IgG |
Sheep
(liquid, PBS, azide, purified) |
ELISA |
Cow |
immunogen = purified ubiquitin purified from bovine red blood cells |
ubiquitin |
- |
|
|
Abcam |
polyclonal
IgG |
Sheep
(liquid, PBS, azide, purified) |
ELISA |
Cow |
immunogen = highly purified ubiquitin from bovine erythrocytes |
ubiquitin |
- |
|
|
Abcam |
polyclonal
IgY |
Chicken
(liquid, PBS, azide, affinity purified) |
WB |
Human |
immunogen = recombinant full length human protein |
ubiquitin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, tris-citrate/phosphate, azide, affinity purified) |
WB |
Cow, Human |
immunogen = commercially sensitive |
epitope = maps to a region between residues 1-50 of human ubiquitin; Swiss-Prot entry P62988 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, tris-citrate/phosphate, azide, affinity purified) |
WB |
Plant (various) |
immunogen = commercially sensitive |
ubiquitin |
- |
|
|
Abcam |
polyclonal
IgG |
Sheep
(liquid, glycerol, PBS, MgCl, ZnCl, azide, protein G purified) |
ELISA, IA, WB |
Cow |
immunogen = full length native protein from rbc's (cow) |
ubiquitin |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, PBS, glycerol, protein G purified) |
ELISA, IP, WB |
Human |
immunogen = synthetic peptide (human) conjugated to KLH |
ubiquitin +1 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, protein A purified) |
IP, WB |
Human |
immunogen = synthetic peptide to human C- terminal ubiquitin +1 conjugated to KLH |
ubiquitin +1 |
- |
|
|
Biogenesis AdB Serotec |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
EM, IH, WB |
Human |
immunogen = purified ubiquitin covalently coupled to KLH using glutaraldehyde |
Ubiquitin; tangles in AD brain, Lewy bodies, Pick bodies |
- |
|
|
Biotrend |
polyclonal
|
Rabbit
(pre-titer) |
IH |
Mammal (various), Human |
ubiquitin isolated from cow euythrocytes |
periphery of senile plaques and neurofibrillay tangles in AD |
- |
|
|
Biotrend |
polyclonal
|
Rabbit
(serum Ig, purified, azide) |
IH |
Mammal (various), Human |
ubiquitin isolated from cow euythrocytes |
periphery of senile plaques and neurofibrillay tangles in AD |
- |
|
|
EnCor Biotechnology, Inc, |
polyclonal
Ig |
Rabbit
(crude serum) |
IF, WB |
- |
glutaraldehyde cross linked ubiquitin in rabbit |
Ubiquitinated inclusion bodies i.e.neurofibrillary tangles of AD, Lewy bodies, Pick bodies |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, ascites, no preservative) |
ELISA, WB |
- |
immunogen = full length denatured protein (purified) (Cow) |
ubiquitin |
- |
|
|
Abcam |
monoclonal
IgG |
Mouse
(liquid, HEPES, BSA, NaCl, glycerol, protein A purified) |
IH, WB |
Primate (various), Cow, Human, Mouse, Rat |
immunogen = full length ubiquitin of bovine origin |
ubiquitin |
- |
|
|
AbD Serotec |
monoclonal
IgG1 |
Mouse
(liquid, IgG fraction, PBS, azide, protein G purified) |
FC, IH |
Bovine, Human |
immunogen = ubiquitin from bovine erythrocytes conj. to bovine gamma globulin |
ubiquitin |
- |
|
|
AbD Serotec |
monoclonal
IgG1 |
Mouse
(liquid, IgG fraction, PBS, azide, protein G purified) |
ELISA, FC, IH, WB |
Bovine, Human |
immunogen = ubiquitin from bovine erythrocytes conj. to bovine gamma globulin |
ubiquitin |
- |
|
|
AbD Serotec |
polyclonal
|
Sheep
(liquid, PBS, glycerol, MgCl2, ZnCl2, azide, protein G purified) |
ELISA |
Bovine, Human |
immunogen = native ubiquitin from erythrocytes |
ubiquitin |
- |
|
|
AbD Serotec |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
IH, WB |
Bovine |
immunogen = synthetic peptide corresp. to part of the native molecule conjugated to KLH |
ubiquitin (76 aa, 8.5 kDa); stains tangle material in Alzheimer brain; also stains Lewy bodies (in Parkinson's disease) and Pick bodies (in Pick's disease). |
- |
|
|
AbD Serotec |
polyclonal
IgG1 |
Sheep
(liquid, PBS, azide, NH2SO4 frac.) |
ELISA |
Bovine, Various species |
immunogen = native, purified >= 90% from bovine erythrocytes |
ubiquitin (76 aa, 8.5 kDa); stains tangle material in Alzheimer brain; also stains Lewy bodies (in Parkinson's disease) and Pick bodies (in Pick's disease). |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin) |
IF, IH, IP, WB |
Bovine, Human, Mouse, Rat |
epitope corr. to aa 1-76 of full length human Ubiquitin |
reactive with ubiquitin, poly-ubiquitin and ubiquitinated proteins |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(liquid, protein-A purified, PBS, glycerol, azide) |
IP, WB |
Human |
immunogen = full length, highly purified, UbcH10 protein |
UbcH10 |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(liquid, protein-A purified, PBS, glycerol, azide) |
IP, WB |
Human |
immunogen = full length, highly purified, UbcH2 protein |
UbcH2 |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(liquid, protein-A purified, PBS, glycerol, azide) |
IP, WB |
Human |
immunogen = full length, highly purified, UbcH7 protein |
UbcH7 |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(liquid, protein-A purified, PBS, glycerol, azide) |
IP, WB |
Human |
immunogen = full length, highly purified, UbcH6 protein |
UbcH6 |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Sheep
(serum, no preservative, lyophilized) |
IH, WB |
Rabbit, Sheep, Human |
immunogen = ubiquitin from bovine red blood cells coupled to chicken gamma globulin |
free ubiquitin and ubiquitin-protein conjugates, tangles and Lewy bodies |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Chicken
(liquid, affinity purified IgG, PBS) |
ELISA, IC, IH, WB |
Human |
immunogen = full length human ubiquitin |
Ubiquitin |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, purified IgG, PBS, azide) |
IH, IP, WB |
Human, Mouse |
immunogen = Ubiquitin Activation Enzyme E-1 |
Ubiquitin Activation Enzyme E-1; antibody does not inhibit the enzymatic activity of E1 |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(liquid, protein-A purified, PBS, glycerol, azide) |
WB |
Mammal (various) |
immunogen = full length, highly purified, UbcH1 protein |
UbcH1 |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(liquid, serum, azide) |
IH, WB |
Human, Mouse, Rat |
immunogen = whole PGP9.5 (ubiquitin C-terminal hydrolase) purified from human brain |
PGP9.5 in neurons and nerve fibers at all levels of the central and peripheral nervous system |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(liquid, serum, azide) |
IH, WB |
Human, Mouse, Rat |
immunogen = whole PGP9.5 (ubiquitin C-terminal hydrolase) purified from human brain |
PGP9.5 in neurons and nerve fibers at all levels of the central and peripheral nervous system |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(liquid, serum, no preservative) |
ELISA, WB |
Human |
immunogen = human ubiquitin- proteosome from erythrocytes |
ubiquitin-proteosome. Reacts with 8-10 bands between 25-35 kDa |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Goat
(liquid, serum, thimerosal) |
ELISA, WB |
Human |
immunogen = synthetic peptide from the N-terminal of human UCH-L1 |
Ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1), 27 kDa by WB |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(liquid, protein-A purified, PBS, glycerol, azide) |
IP, WB |
Human |
immunogen = full length, highly purified, UbcH9 protein |
UbcH9 |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(serum, azide) |
IH, WB |
- |
immunogen = purified ubiquitin covalently coupled to KLH using glutaraldehyde |
ubiqiutin in neurofibrillary tangles, neurites in senile plaques. No reactivity seen to tau, neurofilament or MAP. |
- |
|
|
Biomol Enzo Life Sciences |
monoclonal
IgM |
Mouse
(liquid, purified Ig, PBS, azide) |
IH, WB |
- |
immunogen = poly-ubiquitinylated- lysozyme |
recognises onlypoly ubiquitinylated proteins and not mono ubiquitinylated proteins or free ubiquitin |
- |
|
|
Biomol Enzo Life Sciences |
monoclonal
IgG1 |
Mouse
(liquid, purified Ig, PBS saline, azide) |
ELISA, IF, IP, WB |
Non-specific |
immunogen = crude preparation of poly ubiquitinylated- lysozyme |
recognises mono andpoly ubiquitinylated proteins but not free ubiquitin |
- |
|
|
Novus Biologicals |
monoclonal
IgG1,κ |
Mouse
(lyophilized, supernatant, no preservative) |
ELISA, IH, IP, WB |
Bovine |
- |
ubiquitin proteins (8.5kDa); cellular and nuclear |
- |
|
|
Novus Biologicals |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide) |
FC, IH |
Cow, Human |
immunogen = ubiquitin from bovine erythrocytes conjugated to bovine gamma globulin |
ubiquitin |
- |
|
|
Novus Biologicals |
monoclonal
IgG1,κ |
Mouse
(liquid, ascites, azide) |
ELISA, IH, WB |
Bovine, Chicken, Arabidopsis, Human, Mouse, C. elegans, Drosophila |
immunogen = purified ubiquitin conjugated to KLH with glutaraldehyde |
recognizes ubiquitinated cytoplasmic inclusion bodies |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, KPBS, azide, purified) |
ELISA, WB |
Yeast |
immunogen = ubiquitin coupled to rabbit IgG |
ubiquitin |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(liquid, tris-citrate/phosphate, azide, affinity purified) |
WB |
Plant (various) |
immunogen = synthetic peptide mapping to a region between residues 1-50 of soybean Ubiquitin; Swiss-Prot entry P03993 |
ubiquitin |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(liquid, tris-citrate/phosphate, azide) |
WB |
Bovine, Human |
immunogen = synthetic peptide mapping to a region between residues 1-50 of human Ubiquitin; Swiss-Prot entry P62988. |
ubiquitin |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(liquid, sera) |
ELISA, IF, IH, WB |
Bovine, Chicken, Human, Mouse, C. elegans, Drosophila |
immunogen = gluteraldehyde cross-linked ubiquitin. |
recognizes ubiquitinated inclusion bodies and both the mono- and polyubiquitin forms; also aggresomes |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, protein-A purified) |
IF, IH |
Cow, Human, Mouse, Rat |
immunogen = ubiquitin isolated from cow erythrocytes and conjugated to chicken gammaglobulins |
ubiquitin; also stains with the periphery of senile plaques and neurofibrillay tangles in Alzheimer disease |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, protein-A purified, sucrose, PBS, no preservative) |
IH, WB |
Human |
immunogen = synthetic peptide: corresp. to a region of PIAS3 (Human) |
detects PIAS3 |
- |
|
|
ABGENT |
polyclonal
IgG |
Rabbit
(liquid, protein-G, PBS, azide) |
ELISA, IH, WB |
Human |
immunogen = KLH conjugated synthetic peptide selected from the N-terminal region of human PIAS3 |
detects PIAS3 |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(antisera, affinity purified, PBS, azide) |
IH, WB |
Human, Mouse, Rat |
- |
detects endogenous levels of PIAS3 protein |
- |