|
|
Chemicon and Upstate are Millipore |
monoclonal
IgM |
Mouse
(liquid, purified Ig, phosphate buffer, azide) |
IC, IH |
Chicken, Human, Mouse, Rat |
immunogen = homogenate of white matter of corpus callosum from bovine brain |
Anti-O4 can be used in myelination, demyelination and remyelination studies and in regeneration experiments |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, protein-G purified Ig, phosphate buffer, saline, azide) |
ELISA, IH, WB |
Rat |
immunogen = cell line expressing a truncated form of NG2 |
recognizes the >280 kDa NG2 protein; reacts with native, recombinant and protein from cells which express NG2 |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, ascites fluid, no preservative) |
FC, WB |
Human |
immunogen = human bone marrow stromal cell line, SV40 transformed |
reacts with a 220-240 kDa surface protein whose N-terminal sequence is identical to the rat NG2 chondrotin sulfate proteoglycan molecule |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, supernatant, purified, Tris, fetal calf serum, azide) |
ELISA, IH, RIA, WB |
Bovine, Human, Rat |
immunogen = bovine myelin basic protein (epitope at aa 129-138) |
recognizes MBP |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG |
Mouse
(liquid, PBS, glycerol, azide, protein-A purified) |
FA, WB |
Human |
immunogen = synthetic peptide corresp. to the human myelin basic protein sequence phosphor. at Thr98 and coupled to tuberculin |
reacts with myelin basic protein phosphor. at Thr98, 33kDa; some preparations of MBP contain basal levels of phosphorylated MBP, which are detected |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG |
Mouse
(liquid, PBS, glycerol, HRP conj.) |
ELISA, FA, WB |
Human |
immunogen = synthetic peptide corresp. to the human myelin basic protein sequence phosphor. at Thr98 and coupled to tuberculin |
reacts with myelin basic protein phosphor. at Thr98, 33kDa; some preparations of MBP contain basal levels of phosphorylated MBP, which are detected |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1,κ |
Mouse
(liquid, TrisS, saline, azide, protein-G purified) |
FA, WB |
Various vertebrate |
immunogen = peptide containing the seqeunce PRpTPP which corresp. to the human myelin basic protein sequence phosphor. at Thr98 |
reacts with in-vitro phosphorylated myelin basic protein, ~18-23 kDa |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
ELISA, IH, WB |
Dog, Cow, Sheep, Pig, Human, Mouse, Rat |
immunogen = purified human brain CNPase |
reacts specifically with myelin CNPase; localizes both CNP1 (46kD) and CNP2 (48kD) bands |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, PBS, BSA, azide, protein A purified) |
IC, IF, IH, WB |
Dog, Goat, Rabbit, Cow, Sheep, Pig, Human, Monkey, Mouse, Rat |
immunogen = purified human CNPase |
CNPase |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
DB, ELISA, IC, IF, IH, IP, WB |
Dog, Cow, Sheep, Pig, Human, Mouse, Rat |
immunogen = purified full length native human protein |
reacts specifically with CNPase; localizes both CNP1 (46kD) and CNP2 (48kD) bands |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
WB |
Rabbit, Human, Mouse, Rat |
immunogen = synthetic peptide derived from within residues 350 to the C-terminus of human CNPase, conj. to KLH |
CNPase |
- |
|
|
Abcam |
polyclonal
IgY |
Chicken
(liquid, PBS, azide) |
IC, IF, WB |
Human, Mouse |
immunogen = synthetic peptide conj. to KLH |
CNPase |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
WB |
Human |
immunogen = synthetic peptide derived from within residues 200 - 300 of human CNPase, conj. to KLH |
CNPase, both isoforms (46 and 48 kDa) |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, BSA, azide, protein G purified, prediluted) |
IH |
Cat, Dog, Goat, Rabbit, Cow, Sheep, Pig, Human, Monkey, Mouse, Rat |
immunogen = full length purified human CNPase |
CNPase |
- |
|
|
Abcam |
polyclonal
IgY |
Chicken
(liquid, PBS, azide, protein L purified) |
IC, IF |
Human, Mouse |
immunogen = peptide common to human (NP_149124, NCBI) and mouse (P16330, NCBI) CNPase |
CNPase |
- |
|
|
Abcam |
monoclonal
IgG |
Mouse
(liquid, PBS, azide, protein A purified) |
WB |
Human, Mouse, Rat |
immunogen = synthetic peptide derived from within residues 350 to the C-terminus of human CNPase, conj. to KLH |
CNPase |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = epitope corresp. to aa121-420 mapping at the C-terminus of mouse CNPase |
CNPase, both isoforms 1 (46kDa) and 2 (48 kDa) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, IF, WB |
Mammal (various), Cow, Human, Mouse, Rat |
immunogen = epitope mapping near the N-terminus of human CNPase |
CNPase |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, IF, IP, WB |
Mouse, Rat |
immunogen = epitope mapping within an internal region of mouse CNPase |
CNPase |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, affinity purified) |
ELISA, IF, IH, WB |
Bovine, Cat, Dog, Goat, Porcine, Rabbit, Sheep, Human, Monkey, Mouse, Rat |
immunogen = purified human 2'-3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) |
recognizes proteins of 46kD (CNP1) and 48kD (CNP2), identified as two forms of CNPase |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, PBS, BSA, azide, affinity purified) |
IC, IH, WB |
Bovine, Dog, Porcine, Rabbit, Sheep, Human, Mouse, Rat |
immunogen = purified human 2'-3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) |
recognizes human CNPase, 48 and 46kD polypeptides |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, affinity purified) |
ELISA, IC, IH, WB |
Bovine, Dog, Rabbit, Sheep, Human, Rat |
immunogen = purified human brain CNPase (2'-3'-cyclic nucleotide 3'-phosphodiesterase) whole protein |
recognizes CNPase, 48 and 46kD polypeptides |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, IF, IP, WB |
Mammal (various), Cow, Human, Mouse, Rat |
immunogen = epitope mapping near the N-terminus of human CNPase |
CNPase |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, PBS, no preservative, ammonium sulfate ppt.) |
FC, IH, WB |
Bovine, Dog, Rabbit, Sheep, Pig, Human, Mouse, Rat |
immunogen = native protein |
CNPase; clearly differentiates oligodendrocytes and Schwann cells from meurons, astrocytes etc. |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
immunogen = synthetic protein |
CNPase |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgY |
Chicken
(liquid, PBS, azide, affinity purified) |
IC, IH |
Mouse |
immunogen = synthetic protein conj. KLH; seq. of the synthetic peptide was shared between the human (NP_149124, NCBI) and mouse (P16330, NCBI) |
CNPase |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
immunogen = synthetic protein |
CNPase |
- |
|
|
Cell Signaling |
polyclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol) |
WB |
Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to human CNPase |
endogenous levels of total CNPase |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, protein A purified) |
IC, IH, WB |
Bovine, Dog, Rabbit, Sheep, Pig, Human, Mouse, Rat |
immunogen = purified human brain CNPase |
CNPase, 48 (CNP2) and 46 kD (CNP1); clearly differentiates oligodendrocytes and Schwann cells from neurons, astrocytes, etc. |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, PBS, no preservative, protein A purified) |
IC, IH, WB |
Bovine, Dog, Rabbit, Sheep, Pig, Human, Mouse, Rat |
immunogen = purified human brain CNPase |
CNPase, 48 (CNP2) and 46 kD (CNP1), identified as two isoforms |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
IgY |
Chicken
(liquid, PBS, azide, affinity purified) |
IC, IH |
Human, Mouse |
immunogen = synthetic peptide from human/mouse CNPase |
CNPase |
- |
|
|
Novus Biologicals |
monoclonal
IgG1 |
Mouse
(liquid, ascites, PBS, azide, affinity purified) |
DB, ELISA, IC, IH, IP, WB |
Dog, Rabbit, Cow, Sheep, Pig, Human, Mouse, Rat |
immunogen = full length native protein (purified) (human) |
CNPase localizes both CNP1 (46kD) and CNP2 (48kD) |
- |
|
|
Novus Biologicals |
polyclonal
|
Chicken
(liquid, serum, PBS, azide, affinity purified) |
IC, IH |
Mouse |
immunogen = synthetic peptide was shared between the human (NP_149124, NCBI) and mouse (P16330, NCBI) sequences, conj. KLH |
CNPase |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(liquid, serum, PBS, azide, affinity purified) |
IH, WB |
Rabbit, Sheep, Human, Mouse, Rat |
immunogen = endogenous rabbit 2,3-cyclic nucleotide-3-phospho-diesterase |
CNPase |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(liquid antisera, PBS, azide, affinity purified) |
WB |
Human |
immunogen = residues 68-84 of the 46-48 (doublet) kDa human CNPase protein (epitope: RVIVDKYRD GTKMVSAD) |
detects 2039-cyclic nucleotide phosphodiesterase |
- |
|
|
Upstate and Chemicon are Millipore |
monoclonal
IgG1,κ |
Mouse
(liquid, protein-G purified, phosphate buffer, saline, BSA, no preservatives) |
WB |
Human, Mouse, Rat |
immunogen = peptide corresp. to the human myelin basic protein sequence containing a.a. Thr98 |
recognizes MBP; 18-20 kDa by WB |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, supernatant, Tris/HCL, fetal calf serum, azide) |
ELISA, IH |
Bovine, Rabbit, Sheep, Human, Guinea Pig, Rat |
immunogen = bovine myelin basic protein (epitope in the region of a.a. 119-131) |
recognizes MBP |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, culture supernatant, azide) |
ELISA, IH |
Various species, Human |
immunogen = human myelin basic protein (epitope in between a.a. 67-74: YGSLPQ) |
recognizes MBP |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG2b |
Mouse
(liquid, supernatant, azide) |
IH |
Various vertebrate, Human |
immunogen = human myelin basic protein (recognizes an epitope of MBP between 84-89, a.a. DENPVV) |
recognizes MBP |
- |
|
|
Upstate and Chemicon are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, protein-G purified, phosphate buffer, saline, azide, glycerol) |
IH, WB |
Mouse, Rat |
immunogen = HEK293 cells expressing a truncated integral membrane form of NG2 consisting of amino acids 1592-2222 |
NG2 |
- |
|
|
Abcam |
monoclonal
IgG2a |
Rat
(liquid, tissue culture supernatant, azide) |
ELISA, IH, RIA, WB |
Rabbit, Cow, Sheep, Human, Guinea Pig, Mouse, Rat |
immunogen = full length bovine myelin basic protein (epitope in the region of a.a. 82-87) |
reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Chicken
(liquid, affinity purified, PBS, azide) |
IC, IH |
Mouse |
immunogen = synthetic peptide corresp. to human/mouse PLP protein |
recognizes PLP [Proteolipid Protein] |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG2a |
Mouse
(liquid, protein-A purified, PBS, azide) |
IH, WB |
Mammal (various) |
immunogen = myelin proteolipid protein, synthetic peptide (CGRGTKF) corresp. to the C-terminal peptide |
recognizes myelin PLP; cross reactivity to DM20 |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(liquid, affinity purified, PBS, azide) |
WB |
Rat |
immunogen = Synthetic Olig-2 peptide from rat |
recognizes Olig-2, a band at ~32 kDa in rat brain extract |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(liquid, whole antisera, azide) |
IH |
Human, Rat |
immunogen = human myelin basic protein isolated from normal human brain |
reacts with human myelin basic protein and oligodendrocytes of white matter in brain and spinal cord; also stains peripheral nerves |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(liquid, affinity purified antisera, PBS, azide) |
FC, IH, WB |
Human, Mouse, Rat |
epitope at residues 49-62 of MBP, (DRGAPKR GSGKDSH) |
detects primarily the 22 kDa isoform of myelin basic protein |
- |
|
|
Upstate and Chemicon are Millipore |
monoclonal
IgG1,κ |
Mouse
(liquid, purified ascites, PBS, no preservatives) |
FC, IH, IP, WB |
Human |
immunogen = tissue / cell preparation (Human - a 375P cell crude extract) |
detects MCSP; cell surface marker specific for epidermal stem cells |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG2a |
Mouse
(liquid, protein-A purified, PBS, saline, azide) |
FC, IF, IP |
Human |
immunogen = human M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin-Sepharose™ |
recognizes mature chondroitin sulfate proteoglycan core glycoprotein of 250 kDa as well as precursor polypeptides of 210, 220 and 240 kDa |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(liquid, PBS, azide, protein-A affinity purified) |
ELISA, IC, IH, IP, WB |
Human, Monkey, Mouse, Rat |
immunogen = immunoaffinity purified NG2 Chondroitin Sulfate Proteoglycan from rat |
identifies both the intact proteoglycan and the core protein by Western blot and ELISA |
- |
|
|
R&D Systems |
monoclonal
IgM |
Mouse
(filtered solution in PBS, trehalose, lyophilized) |
FC, IH |
Chicken, Human, Mouse, Rat |
immunogen = white matter of corpus callosum from bovine brain |
recognizes Oligodendrocyte cell surface marker O1 |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
Ig |
Rabbit
(liquid, purified Ig, Tris, glycine) |
IC, IH, IP, WB |
Human, Mouse, Rat |
immunogen = recombinant mouse Olig-2 |
recognizes Oligodendrocyte marker Olig-2; recognizes the ~32 kDa Olig-2 protein by Western blot |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum, azide) |
IH |
Human, Rat |
immunogen = myelin basic protein isolated from normal human brain |
reacts with human myelin basic protein and oligodendrocytes of white matter in brain and spinal cord. Grey matter stains weakly. This antibody also stains peripheral nerves |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG3 |
Mouse
(liquid, purified, Potassium Phosphate, saline, azide) |
ELISA, IC, IH, WB |
Bovine, Rabbit, Chicken, Human, Mouse, Rat |
immunogen = Synaptic plasma membranes from bovine hippocampus |
recognizes Galactocerebroside (GalC), sulfatide, psychosine and other galactolipids. Cross-reacts with the sulfatide ester of GalC, but to a 16-fold lesser extent. No cross-reactivity with sphingosine, ceramide, mixed ganglioside or glucocerebroside. Binds specifically with oligodendrocytes and Schwann cellsGalC is a very useful, specific marker for oligodendroglial lineage |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, protein-A purified, phosphate buffer, saline, azide) |
IC, IH, WB |
Bovine, Dog, Rabbit, Sheep, Pig, Human, Mouse, Rat |
immunogen = purified human brain CNPase |
recognizes CNPase, 48 and 46 kD polypeptides by SDS-PAGE; differentiates clearly oligodendrocytes and Schwann cells from neurons, astrocytes, etc |
- |
|
|
AnaSpec |
polyclonal
|
Rabbit
(liquid, affinity purified, PBS, BSA, azide) |
IH |
Human |
immunogen = a Myelin basic protein isolated from a human brain |
reacts with Myelin Basic Protein |
- |
|
|
AnaSpec |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, PBS, azide) |
WB |
Pig, Human, Mouse, Rat |
immunogen = a synthetic peptide corresponding to the N-terminus of human myelin basic protein |
reacts with MBP-11 |
- |
|
|
AnaSpec |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, PBS, azide) |
WB |
Human, Mouse, Rat |
immunogen = a synthetic peptide corresponding to the amino acid sequence 139-151 of mouse proteolipid protein |
recognizes PLP |
- |
|
|
AnaSpec |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, PBS, azide) |
ELISA, WB |
Human, Mouse, Rat |
immunogen = a synthetic peptide corresponding to the amino acid sequence 178-191 of mouse proteolipid protein |
recognizes PLP |
- |
|
|
StemCell Technologies |
monoclonal
IgM |
Mouse
(liquid, purified Ig, phosphate buffer, azide) |
IF |
Mouse |
immunogen = homogenate of white matter of corpus callosum from bovine brain |
O4 can be used to detect oligo dendrocytes in as early as day 3 cellcultures of brains from mouse embryos |
- |
|
|
EMD Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, azide, undiluted ascites) |
ELISA, IC, IH, WB |
Bovine, Dog, Porcine, Sheep, Human, Mouse, Rat |
immunogen = purified, human myelin CNPase |
recognizes the ~46 and ~48 kDa subunits of the 94 kDa myelin CNPase protein |
- |
|
|
None |
monoclonal
IgG1 |
Mouse
(liquid, ascites, high titer) |
ELISA, IH, WB |
Bovine, Dog, Sheep, Pig, Human, Mouse, Rat |
- |
reacts with the 46 and 48 Mr subunit of the 94 Mr myelin CNPase dimer; detects developing and adult myelin, developing oligodendrocytes and Schwann cells |
Discontinued |