O4 (81 /mAB O4)
Chemicon and Upstate are Millipore
monoclonal
IgM
Mouse
(liquid, purified Ig, phosphate buffer, azide)
-
Chicken, Human, Mouse, Rat
immunogen = homogenate of white matter of corpus callosum from bovine brain
Anti-O4 can be used in myelination, demyelination and remyelination studies and in regeneration experiments
-
myelin CNPase (SMI91)
None
monoclonal
IgG1
Mouse
(liquid, ascites, high titer)
-
Bovine, Dog, Sheep, Pig, Human, Mouse, Rat
-
reacts with the 46 and 48 Mr subunit of the 94 Mr myelin CNPase dimer; detects developing and adult myelin, developing oligodendrocytes and Schwann cells
Discontinued
NG2, Chondroitin Sulfate Proteoglycan
Chemicon and Upstate are Millipore
monoclonal
IgG1
Mouse
(liquid, protein-G purified Ig, phosphate buffer, saline, azide)
-
Rat
immunogen = cell line expressing a truncated form of NG2
recognizes the >280 kDa NG2 protein; reacts with native, recombinant and protein from cells which express NG2
-
NG2, Chondroitin Sulfate Proteoglycan (7.1)
Chemicon and Upstate are Millipore
monoclonal
IgG1
Mouse
(liquid, ascites fluid, no preservative)
-
Human
immunogen = human bone marrow stromal cell line, SV40 transformed
reacts with a 220-240 kDa surface protein whose N-terminal sequence is identical to the rat NG2 chondrotin sulfate proteoglycan molecule
-
MBP (1)
Chemicon and Upstate are Millipore
monoclonal
IgG1
Mouse
(liquid, supernatant, purified, Tris, fetal calf serum, azide)
-
Bovine, Human, Rat
immunogen = bovine myelin basic protein (epitope at aa 129-138)
recognizes MBP
-
MBP phospho (P12)
Chemicon and Upstate are Millipore
monoclonal
IgG
Mouse
(liquid, PBS, glycerol, azide, protein-A purified)
-
Human
immunogen = synthetic peptide corresp. to the human myelin basic protein sequence phosphor. at Thr98 and coupled to tuberculin
reacts with myelin basic protein phosphor. at Thr98, 33kDa; some preparations of MBP contain basal levels of phosphorylated MBP, which are detected
-
MBP phospho (P12)
Chemicon and Upstate are Millipore
monoclonal
IgG
Mouse
(liquid, PBS, glycerol, HRP conj.)
-
Human
immunogen = synthetic peptide corresp. to the human myelin basic protein sequence phosphor. at Thr98 and coupled to tuberculin
reacts with myelin basic protein phosphor. at Thr98, 33kDa; some preparations of MBP contain basal levels of phosphorylated MBP, which are detected
-
MBP phospho (BK403)
Chemicon and Upstate are Millipore
monoclonal
IgG1 ,κ
Mouse
(liquid, TrisS, saline, azide, protein-G purified)
-
Various vertebrate
immunogen = peptide containing the seqeunce PRpTPP which corresp. to the human myelin basic protein sequence phosphor. at Thr98
reacts with in-vitro phosphorylated myelin basic protein, ~18-23 kDa
-
myelin CNPase (SMI-91)
Abcam
monoclonal
IgG1
Mouse
(liquid, ascites, azide)
-
Dog, Cow, Sheep, Pig, Human, Mouse, Rat
immunogen = purified human brain CNPase
reacts specifically with myelin CNPase; localizes both CNP1 (46kD) and CNP2 (48kD) bands
-
CNPase (11-5B)
Abcam
monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide, protein A purified)
-
Dog, Goat, Rabbit, Cow, Sheep, Pig, Human, Monkey, Mouse, Rat
immunogen = purified human CNPase
CNPase
-
CNPase (11-5B)
Abcam
monoclonal
IgG1
Mouse
(liquid, ascites, azide)
-
Dog, Cow, Sheep, Pig, Human, Mouse, Rat
immunogen = purified full length native human protein
reacts specifically with CNPase; localizes both CNP1 (46kD) and CNP2 (48kD) bands
-
CNPase
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
-
Rabbit, Human, Mouse, Rat
immunogen = synthetic peptide derived from within residues 350 to the C-terminus of human CNPase, conj. to KLH
CNPase
-
CNPase
Abcam
polyclonal
IgY
Chicken
(liquid, PBS, azide)
-
Human, Mouse
immunogen = synthetic peptide conj. to KLH
CNPase
-
CNPase
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
-
Human
immunogen = synthetic peptide derived from within residues 200 - 300 of human CNPase, conj. to KLH
CNPase, both isoforms (46 and 48 kDa)
-
CNPase (SPM238)
Abcam
monoclonal
IgG1 , κ
Mouse
(liquid, PBS, BSA, azide, protein G purified, prediluted)
-
Cat, Dog, Goat, Rabbit, Cow, Sheep, Pig, Human, Monkey, Mouse, Rat
immunogen = full length purified human CNPase
CNPase
-
CNPase
Abcam
polyclonal
IgY
Chicken
(liquid, PBS, azide, protein L purified)
-
Human, Mouse
immunogen = peptide common to human (NP_149124, NCBI) and mouse (P16330, NCBI) CNPase
CNPase
-
CNPase (mABcam 44289)
Abcam
monoclonal
IgG
Mouse
(liquid, PBS, azide, protein A purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide derived from within residues 350 to the C-terminus of human CNPase, conj. to KLH
CNPase
-
CNPase (M-300)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = epitope corresp. to aa121-420 mapping at the C-terminus of mouse CNPase
CNPase, both isoforms 1 (46kDa) and 2 (48 kDa)
-
CNPase (N-19)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
-
Mammal (various), Cow, Human, Mouse, Rat
immunogen = epitope mapping near the N-terminus of human CNPase
CNPase
-
CNPase (K-14)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
-
Mouse, Rat
immunogen = epitope mapping within an internal region of mouse CNPase
CNPase
-
CNPase
LifeSpan Biosciences, Inc.
monoclonal
IgG1
Mouse
(liquid, PBS, azide, affinity purified)
-
Bovine, Cat, Dog, Goat, Porcine, Rabbit, Sheep, Human, Monkey, Mouse, Rat
immunogen = purified human 2'-3'-cyclic nucleotide 3'-phosphodiesterase (CNPase)
recognizes proteins of 46kD (CNP1) and 48kD (CNP2), identified as two forms of CNPase
-
CNPase
LifeSpan Biosciences, Inc.
monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide, affinity purified)
-
Bovine, Dog, Porcine, Rabbit, Sheep, Human, Mouse, Rat
immunogen = purified human 2'-3'-cyclic nucleotide 3'-phosphodiesterase (CNPase)
recognizes human CNPase, 48 and 46kD polypeptides
-
CNPase
LifeSpan Biosciences, Inc.
monoclonal
IgG1
Mouse
(liquid, PBS, azide, affinity purified)
-
Bovine, Dog, Rabbit, Sheep, Human, Rat
immunogen = purified human brain CNPase (2'-3'-cyclic nucleotide 3'-phosphodiesterase) whole protein
recognizes CNPase, 48 and 46kD polypeptides
-
CNPase (E-18)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
-
Mammal (various), Cow, Human, Mouse, Rat
immunogen = epitope mapping near the N-terminus of human CNPase
CNPase
-
CNPase (41/7)
LifeSpan Biosciences, Inc.
monoclonal
IgG1
Mouse
(liquid, PBS, no preservative, ammonium sulfate ppt.)
-
Bovine, Dog, Rabbit, Sheep, Pig, Human, Mouse, Rat
immunogen = native protein
CNPase; clearly differentiates oligodendrocytes and Schwann cells from meurons, astrocytes etc.
-
CNPase
LifeSpan Biosciences, Inc.
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic protein
CNPase
-
CNPase
LifeSpan Biosciences, Inc.
polyclonal
IgY
Chicken
(liquid, PBS, azide, affinity purified)
-
Mouse
immunogen = synthetic protein conj. KLH; seq. of the synthetic peptide was shared between the human (NP_149124, NCBI) and mouse (P16330, NCBI)
CNPase
-
CNPase
LifeSpan Biosciences, Inc.
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic protein
CNPase
-
CNPase
Cell Signaling
polyclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to human CNPase
endogenous levels of total CNPase
-
CNPase (11-5B)
Chemicon and Upstate are Millipore
monoclonal
IgG1
Mouse
(liquid, PBS, azide, protein A purified)
-
Bovine, Dog, Rabbit, Sheep, Pig, Human, Mouse, Rat
immunogen = purified human brain CNPase
CNPase, 48 (CNP2) and 46 kD (CNP1); clearly differentiates oligodendrocytes and Schwann cells from neurons, astrocytes, etc.
-
CNPase (11-5B)
Chemicon and Upstate are Millipore
monoclonal
IgG1
Mouse
(liquid, PBS, no preservative, protein A purified)
-
Bovine, Dog, Rabbit, Sheep, Pig, Human, Mouse, Rat
immunogen = purified human brain CNPase
CNPase, 48 (CNP2) and 46 kD (CNP1), identified as two isoforms
-
CNPase
Chemicon and Upstate are Millipore
polyclonal
IgY
Chicken
(liquid, PBS, azide, affinity purified)
-
Human, Mouse
immunogen = synthetic peptide from human/mouse CNPase
CNPase
-
CNPase (11-58)
Novus Biologicals
monoclonal
IgG1
Mouse
(liquid, ascites, PBS, azide, affinity purified)
-
Dog, Rabbit, Cow, Sheep, Pig, Human, Mouse, Rat
immunogen = full length native protein (purified) (human)
CNPase localizes both CNP1 (46kD) and CNP2 (48kD)
-
CNPase
Novus Biologicals
polyclonal
Chicken
(liquid, serum, PBS, azide, affinity purified)
-
Mouse
immunogen = synthetic peptide was shared between the human (NP_149124, NCBI) and mouse (P16330, NCBI) sequences, conj. KLH
CNPase
-
CNPase
Novus Biologicals
polyclonal
Rabbit
(liquid, serum, PBS, azide, affinity purified)
-
Rabbit, Sheep, Human, Mouse, Rat
immunogen = endogenous rabbit 2,3-cyclic nucleotide-3-phospho-diesterase
CNPase
-
CNPase
Novus Biologicals
polyclonal
Rabbit
(liquid antisera, PBS, azide, affinity purified)
-
Human
immunogen = residues 68-84 of the 46-48 (doublet) kDa human CNPase protein (epitope: RVIVDKYRD GTKMVSAD)
detects 2039-cyclic nucleotide phosphodiesterase
-
MBP (SKB3)
Upstate and Chemicon are Millipore
monoclonal
IgG1 ,κ
Mouse
(liquid, protein-G purified, phosphate buffer, saline, BSA, no preservatives)
-
Human, Mouse, Rat
immunogen = peptide corresp. to the human myelin basic protein sequence containing a.a. Thr98
recognizes MBP; 18-20 kDa by WB
-
MBP (2)
Chemicon and Upstate are Millipore
monoclonal
IgG1
Mouse
(liquid, supernatant, Tris/HCL, fetal calf serum, azide)
-
Bovine, Rabbit, Sheep, Human, Guinea Pig, Rat
immunogen = bovine myelin basic protein (epitope in the region of a.a. 119-131)
recognizes MBP
-
MBP (26)
Chemicon and Upstate are Millipore
monoclonal
IgG1
Mouse
(liquid, culture supernatant, azide)
-
Various species, Human
immunogen = human myelin basic protein (epitope in between a.a. 67-74: YGSLPQ)
recognizes MBP
-
MBP
Chemicon and Upstate are Millipore
monoclonal
IgG2b
Mouse
(liquid, supernatant, azide)
-
Various vertebrate, Human
immunogen = human myelin basic protein (recognizes an epitope of MBP between 84-89, a.a. DENPVV)
recognizes MBP
-
NG2, Chondroitin Sulfate Proteoglycan (132.38)
Upstate and Chemicon are Millipore
monoclonal
IgG1
Mouse
(liquid, protein-G purified, phosphate buffer, saline, azide, glycerol)
-
Mouse, Rat
immunogen = HEK293 cells expressing a truncated integral membrane form of NG2 consisting of amino acids 1592-2222
NG2
-
MBP (12)
Abcam
monoclonal
IgG2a
Rat
(liquid, tissue culture supernatant, azide)
-
Rabbit, Cow, Sheep, Human, Guinea Pig, Mouse, Rat
immunogen = full length bovine myelin basic protein (epitope in the region of a.a. 82-87)
reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP
-
PLP
Chemicon and Upstate are Millipore
polyclonal
Chicken
(liquid, affinity purified, PBS, azide)
-
Mouse
immunogen = synthetic peptide corresp. to human/mouse PLP protein
recognizes PLP [Proteolipid Protein]
-
Myelin PLP, C-term (PLPC1)
Chemicon and Upstate are Millipore
monoclonal
IgG2a
Mouse
(liquid, protein-A purified, PBS, azide)
-
Mammal (various)
immunogen = myelin proteolipid protein, synthetic peptide (CGRGTKF) corresp. to the C-terminal peptide
recognizes myelin PLP; cross reactivity to DM20
-
Olig-2
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(liquid, affinity purified, PBS, azide)
-
Rat
immunogen = Synthetic Olig-2 peptide from rat
recognizes Olig-2, a band at ~32 kDa in rat brain extract
-
MBP
Novus Biologicals
polyclonal
Rabbit
(liquid, whole antisera, azide)
-
Human, Rat
immunogen = human myelin basic protein isolated from normal human brain
reacts with human myelin basic protein and oligodendrocytes of white matter in brain and spinal cord; also stains peripheral nerves
-
MBP
Novus Biologicals
polyclonal
Rabbit
(liquid, affinity purified antisera, PBS, azide)
-
Human, Mouse, Rat
epitope at residues 49-62 of MBP, (DRGAPKR GSGKDSH)
detects primarily the 22 kDa isoform of myelin basic protein
-
MCSP /NG2 (LHM 2)
Upstate and Chemicon are Millipore
monoclonal
IgG1 ,κ
Mouse
(liquid, purified ascites, PBS, no preservatives)
-
Human
immunogen = tissue / cell preparation (Human - a 375P cell crude extract)
detects MCSP; cell surface marker specific for epidermal stem cells
-
Chondroitin Sulfate Proteoglycan (9.2.27)
Chemicon and Upstate are Millipore
monoclonal
IgG2a
Mouse
(liquid, protein-A purified, PBS, saline, azide)
-
Human
immunogen = human M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin-Sepharose™
recognizes mature chondroitin sulfate proteoglycan core glycoprotein of 250 kDa as well as precursor polypeptides of 210, 220 and 240 kDa
-
NG2, Chondroitin Sulfate Proteoglycan
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(liquid, PBS, azide, protein-A affinity purified)
-
Human, Monkey, Mouse, Rat
immunogen = immunoaffinity purified NG2 Chondroitin Sulfate Proteoglycan from rat
identifies both the intact proteoglycan and the core protein by Western blot and ELISA
-
O1 (O1)
R&D Systems
monoclonal
IgM
Mouse
(filtered solution in PBS, trehalose, lyophilized)
-
Chicken, Human, Mouse, Rat
immunogen = white matter of corpus callosum from bovine brain
recognizes Oligodendrocyte cell surface marker O1
-
Olig-2
Chemicon and Upstate are Millipore
polyclonal
Ig
Rabbit
(liquid, purified Ig, Tris, glycine)
-
Human, Mouse, Rat
immunogen = recombinant mouse Olig-2
recognizes Oligodendrocyte marker Olig-2; recognizes the ~32 kDa Olig-2 protein by Western blot
-
MBP
Abcam
polyclonal
IgG
Rabbit
(liquid, whole antiserum, azide)
-
Human, Rat
immunogen = myelin basic protein isolated from normal human brain
reacts with human myelin basic protein and oligodendrocytes of white matter in brain and spinal cord. Grey matter stains weakly. This antibody also stains peripheral nerves
-
Galactocerebroside (mGalC)
Chemicon and Upstate are Millipore
monoclonal
IgG3
Mouse
(liquid, purified, Potassium Phosphate, saline, azide)
-
Bovine, Rabbit, Chicken, Human, Mouse, Rat
immunogen = Synaptic plasma membranes from bovine hippocampus
recognizes Galactocerebroside (GalC), sulfatide, psychosine and other galactolipids. Cross-reacts with the sulfatide ester of GalC, but to a 16-fold lesser extent. No cross-reactivity with sphingosine, ceramide, mixed ganglioside or glucocerebroside. Binds specifically with oligodendrocytes and Schwann cellsGalC is a very useful, specific marker for oligodendroglial lineage
-
CNPase (11-5B)
Chemicon and Upstate are Millipore
monoclonal
IgG1
Mouse
(liquid, protein-A purified, phosphate buffer, saline, azide)
-
Bovine, Dog, Rabbit, Sheep, Pig, Human, Mouse, Rat
immunogen = purified human brain CNPase
recognizes CNPase, 48 and 46 kD polypeptides by SDS-PAGE; differentiates clearly oligodendrocytes and Schwann cells from neurons, astrocytes, etc
-
Myelin Basic Protein
Anaspec
polyclonal
Rabbit
(liquid, affinity purified, PBS, BSA, azide)
-
Human
immunogen = a Myelin basic protein isolated from a human brain
reacts with Myelin Basic Protein
-
MBP-11
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Pig, Human, Mouse, Rat
immunogen = a synthetic peptide corresponding to the N-terminus of human myelin basic protein
reacts with MBP-11
-
Proteolipid protein /PLP
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human, Mouse, Rat
immunogen = a synthetic peptide corresponding to the amino acid sequence 139-151 of mouse proteolipid protein
recognizes PLP
-
Proteolipid protein /PLP
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human, Mouse, Rat
immunogen = a synthetic peptide corresponding to the amino acid sequence 178-191 of mouse proteolipid protein
recognizes PLP
-
O4 (81)
StemCell Technologies
monoclonal
IgM
Mouse
(liquid, purified Ig, phosphate buffer, azide)
-
Mouse
immunogen = homogenate of white matter of corpus callosum from bovine brain
O4 can be used to detect oligo dendrocytes in as early as day 3 cellcultures of brains from mouse embryos
-
myelin CNPase (SMI-91)
EMD Biosciences, Inc.
monoclonal
IgG1
Mouse
(liquid, azide, undiluted ascites)
-
Bovine, Dog, Porcine, Sheep, Human, Mouse, Rat
immunogen = purified, human myelin CNPase
recognizes the ~46 and ~48 kDa subunits of the 94 kDa myelin CNPase protein
-