|
|
Novus Biologicals |
monoclonal
IgG2a, κ |
Mouse
(liquid, ascites, no preservative, purified) |
ELISA, WB |
Human |
immunogen = AKT3 (AAD29089, 100-189 aa) partial recombinant protein with GST tag |
Akt3, recombinant protein |
- |
|
|
Novus Biologicals |
monoclonal
(2D2B11H5) |
Mouse
(liquid, ascites, PBS) |
ELISA, WB |
Human |
Immunogen = Ni-NTA purified truncated recombinant Akt3 expressed in E. Coli strain BL21 (DE3) |
Akt3 |
- |
|
|
Novus Biologicals |
monoclonal
IgG2a, κ |
Mouse
(liquid, ascites, no preservative, purified) |
ELISA, IF, WB |
Human |
immunogen = AKT3 (AAD29089, 100-189 aa) partial recombinant protein with GST tag |
Akt3, recombinant protein |
- |
|
|
Novus Biologicals |
monoclonal
IgG, κ |
Mouse
(liquid, ascites, no preservative, purified) |
ELISA, WB |
Human |
immunogen = AKT3 (AAD29089, 100-189 aa) partial recombinant protein with GST tag |
Akt3, recombinant protein |
- |
|
|
Novus Biologicals |
monoclonal
IgG2a, κ |
Mouse
(liquid, ascites, no preservative, purified) |
ELISA, WB |
Human |
immunogen = AKT3 (AAD29089, 100-189 aa) partial recombinant protein with GST tag |
Akt3, recombinant protein |
- |
|
|
Novus Biologicals |
polyclonal
|
Mouse
(liquid, serum, glycerol, no preservative) |
ELISA, WB |
Human |
immunogen = AKT3 (AAD29089, 100-189 aa) partial recombinant protein with GST tag |
Akt3 |
- |
|
|
Novus Biologicals |
monoclonal
IgG2a, κ |
Mouse
(liquid, ascites, no preservative, purified) |
ELISA, WB |
Human |
immunogen = AKT3 (AAD29089, 100-189 aa) partial recombinant protein with GST tag |
Akt3, recombinant protein |
- |
|
|
Novus Biologicals |
polyclonal
|
Mouse
(liquid, serum, glycerol, no preservative) |
ELISA, WB |
Human |
immunogen = AKT1 (AAH00479, 381-480 aa) partial recombinant protein with GST tag. |
AKT1 |
- |
|
|
Novus Biologicals |
polyclonal
|
Mouse
(liquid, serum, glycerol, no preservative) |
ELISA, WB |
Human |
immunogen = AKT2 (AAA58364, 100-189 aa) partial recombinant protein with GST tag |
AKT2 |
- |
|
|
Novus Biologicals |
monoclonal
IgG1, κ |
Mouse
(liquid, ascites, PBS, no preservative) |
ELISA, WB |
Human |
immunogen = AKT2 (AAA58364, 100-189 aa) partial recombinant protein with GST tag |
AKT2 |
- |
|
|
Novus Biologicals |
monoclonal
IgG1, κ |
Mouse
(liquid, ascites, PBS, no preservative) |
ELISA, WB |
Human |
immunogen = AKT2 (AAA58364, 100-189 aa) partial recombinant protein with GST tag |
AKT2, recombinant protein |
- |
|
|
Novus Biologicals |
monoclonal
IgG mix κ |
Mouse
(liquid, ascites, PBS, no preservative) |
ELISA, WB |
Human |
immunogen = AKT2 (AAA58364, 100-189 aa) partial recombinant protein with GST tag |
AKT2, recombinant protein |
- |
|
|
Novus Biologicals |
monoclonal
IgG1, κ |
Mouse
(liquid, ascites, PBS, no preservative) |
ELISA, WB |
Human |
immunogen = AKT2 (AAA58364, 100-189 aa) partial recombinant protein with GST tag |
AKT2, recombinant protein |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, azide) |
ELISA, IH, WB |
Human |
Immunogen = synthetic phosphopeptide corresp. to residues surrounding Serine 124 of human AKT1 |
AKT1 phosporylated at Serine 124 |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, azide) |
ELISA, WB |
Human |
Immunogen = synthetic phosphopeptide corresp. to residues surrounding Tyr326 of human AKT1 |
AKT1 phosporylated at Tyr326 |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, azide) |
DB, ELISA |
Human |
Immunogen = synthetic phosphopeptide corresp. to residues surrounding Serine 474 of human AKT2 |
AKT2 phosporylated at Serine 474 |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, azide) |
ELISA, WB |
Human |
Immunogen = synthetic phosphopeptide corresp. to residues surrounding Tyr326 of human AKT1 |
AKT1 phosporylated at Tyr326 |
- |
|
|
Novus Biologicals |
monoclonal
IgG |
Rabbit
(liquid, ascites, PBS, glycerol, BSA, azide, affinity purified) |
IF, WB |
Human, Mouse |
Immunogen = synthetic peptide derived from the region of human Akt that contains Ser473 |
Akt1, Akt2, Akt3 |
- |
|
|
BD Pharmingen |
monoclonal
IgG1 |
Mouse
(liquid buffered solution, azide) |
IP, WB |
Human, Mouse, Rat |
Immunogen = synthetic phospho-peptide corresponding to residues 464-479 of Akt1 phosphorylated at serine 473 |
Akt phosphorylated at Ser473 (Akt1) and Ser472 (Akt3). May also recognize Akt2 phosporylated at Ser474 but has not been tested. |
- |
|
|
BD Pharmingen |
monoclonal
IgG2A |
Mouse
(liquid, BSA, glycerol, azide, supernate or ascites, affinity purified) |
IF, WB |
Dog, Human, Mouse, Rat, Drosophila |
Immunogen = human Akt1 aa 1-480 |
Akt/PKBα (59kDa) |
- |
|
|
BD Pharmingen |
monoclonal
IgG1, κ |
Mouse
(liquid, BSA, glycerol, azide, supernate or ascites, affinity purified) |
IF, WB |
Dog, Human, Mouse, Rat |
Immunogen = human Akt1 aa 1-480 |
Akt/PKBα (59kDa) regardless of phosphorylation status |
- |
|
|
BD Pharmingen |
monoclonal
IgG1 |
Mouse
(liquid, BSA, glycerol, azide, supernate or ascites, affinity purified) |
IF, WB |
Dog, Human, Mouse, Rat |
Immunogen = human Akt1 aa 71-184 |
Akt/PKBα (59kDa) |
- |
|
|
BD Pharmingen |
monoclonal
IgG1,κ |
Mouse
(liquid, affinity purified, buffered, azide) |
WB |
Human, Mouse |
immunogen = Phosphorylated peptide of the region including the threonine 308 of human and mouseAkt1 |
recognizes Akt phosphorylated at Threonine 308 |
- |
|
|
BD Pharmingen |
monoclonal
IgG1,κ |
Mouse
(liquid, affinity purified, BSA, buffered, azide, conj. PE) |
WB |
Human, Mouse |
immunogen = phosphorylated peptide of the region including the threonine 308 of human and mouseAkt1 |
recognizes Akt phosphorylated at Threonine 308 |
- |
|
|
BD Pharmingen |
kit
|
Unknown Host
(antibodies to: Bad, GSK-3b, Insulin Receptor b, IRS-1, p70s6k, PI3-Kinase, PKBa/Akt, PKB Kinase/PDK1, PP2A Catalytic a) |
IF, WB |
Dog, Chicken, Human, Mouse, Rat |
- |
- |
- |
|
|
BD Pharmingen |
kit
|
Unknown Host
(capture bead A4: phospho Akt1 Ser473) |
IA |
Human, Mouse |
- |
Akt1, phos Ser473 |
- |
|
|
BD Pharmingen |
kit
|
Unknown Host
(capture bead A4: phospho Akt1 Thr308) |
IA |
Human |
- |
Akt1, phos Thr308 |
- |
|
|
BD Pharmingen |
kit
|
Unknown Host
(total Akt1 capture bead A4) |
FC, IA |
Human, Mouse, Rat |
- |
total Akt1 |
- |
|
|
Promega |
polyclonal
IgG |
Rabbit
(liquid, PBS, gentamicin, affinity purified) |
IC, IH, WB |
- |
Immunogen = peptide from the singly phosphorylated Ser473 region from the C-terminus of Akt-1 protein |
Ser473 phosphorylated isoforms of Akt; no cross-reaction with nonphosphorylated Akt |
- |
|
|
ProSci |
polyclonal
IgY |
Chicken
(liquid, PBS, no preservative) |
IH, WB |
Bovine, Human, Mouse, Rat, Xenopus |
Immunogen = aa2-200 of Akt1 |
Akt1 |
- |
|
|
ProSci |
polyclonal
IgY |
Chicken
(liquid, PBS, no preservative) |
ELISA, IH, WB |
Human, Mouse, Rat |
Immunogen = amino acid 106-122 of Akt1 |
Akt1 |
- |
|
|
ProSci |
polyclonal
IgY |
Chicken
(liquid, PBS, no preservative) |
ELISA, IH, WB |
Human, Mouse, Rat |
Immunogen = aa108-124 of Akt2 |
Akt2 |
- |
|
|
ProSci |
polyclonal
IgY |
Chicken
(liquid, PBS, no preservative) |
ELISA, IH, WB |
Human, Mouse, Rat |
Immunogen = amino acids 124-144 of Akt3 |
Akt3 |
- |
|
|
ProSci |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
WB |
Human, Mouse, Rat |
Immunogen = 16 amino acid peptide from near the N-terminus of human Akt1 |
Akt1 |
- |
|
|
ProSci |
polyclonal
|
Rabbit
(liquid, PBS, glycerol, BSA, affinity purified) |
WB |
Human, Mouse |
Immunogen = synthesized phosphopeptide derived from the region of human Akt1 that contains threonine 308 |
Akt1 (100% homologous), expect Akt2 (100% homologous) and Akt3 (92% homologous) |
- |
|
|
ProSci |
polyclonal
IgG |
Rabbit
(lyophilized, PBS, sucrose, no preservative, affinity purified) |
WB |
Human, Mouse |
Immunogen = synthetic peptide corresp. to a region of human AKT1 |
Akt1 |
- |
|
|
ProSci |
polyclonal
IgG |
Rabbit
(lyophilized, PBS, sucrose, no preservative, protein A/G affinity purified) |
WB |
Human, Mouse |
Immunogen = synthetic peptide corresp. to a region of human AKT1 |
Akt1 |
- |
|
|
ProSci |
polyclonal
IgY |
Chicken
(liquid, PBS, no preservative, affinity purified, biotinylated) |
WB |
Bovine, Human, Mouse, Rat, Xenopus |
Immunogen = aa2-200 of Akt1 |
Akt1, detects RAC-alpha serine/threonine-protein kinase |
- |
|
|
ProSci |
polyclonal
IgY |
Chicken
(liquid, PBS, no preservative, affinity purified, biotinylated) |
ELISA, WB |
Human |
Immunogen = aa108-124 of Akt2 |
Akt2, detects RAC-beta serine/threonine-protein kinase |
- |
|
|
ProSci |
polyclonal
IgY |
Goat
(liquid, TBS, BSA, azide, purified) |
ELISA, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide at an internal region of AKT3 |
Akt3 |
- |
|
|
R&D Systems |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
IH, WB |
Human, Mouse, Rat |
Immunogen = synthetic phospho-peptide corresponding to residues surrounding T308 of human, rat and mouse Akt1 |
Phospho-Akt (T308), phospho-Akt2 (T309), phospho-Akt3 (T305) |
- |
|
|
R&D Systems |
kit
|
Unknown Host
(mouse anti-phospho Akt (pan) (S473), rabbit anti-phospho Akt (pan) (S473)) |
ELISA, IA |
Human, Mouse, Rat |
- |
endogenous and recombinant Akt phosphorylated at Ser473 |
- |
|
|
R&D Systems |
kit
|
Unknown Host
(rabbit anti-AktpS473, mouse anti-total Akt) |
ELISA |
Human, Mouse, Rat |
- |
Phospho-Akt (S473) |
- |
|
|
R&D Systems |
kit
|
Unknown Host
(capture antibody = anti-phopho-Akt (pan) (S473); detection antibody = mouse anti-phopho-Akt (pan) (S473)) |
ELISA |
Human, Mouse, Rat |
- |
Phospho-Akt (S473) |
- |
|
|
R&D Systems |
monoclonal
IgG2b |
Mouse
(liquid, PBS, azide, conj. to allophycocyanin (APC), FITC or phyoerythrin (PE)) |
FC |
Human, Mouse, Rat |
Immunogen = E. coli derived recombinant human v-akt murine thymoma viral oncogene homolog 1 (aa 2-480) |
Akt1, Akt2, Akt3 |
- |
|
|
R&D Systems |
kit
|
Unknown Host
(lyophilized, PBS, azide, affinity purified, conj. FITC or PE) |
FC |
Human, Mouse, Rat |
- |
Phospho-Akt1 (S473), phospho-Akt2 (S474), phospho-Akt3 (S472) |
- |
|
|
R&D Systems |
monoclonal
IgG2a |
Mouse
(lyophilized, PBS, trehalose, Protein G purified) |
WB |
Human, Mouse, Rat |
Immunogen = purified E. coli derived full-length (480aa)recombinant human Akt1 |
Akt1; not recombinant Akt2 or Akt3 in WB |
- |
|
|
R&D Systems |
monoclonal
IgG1 |
Mouse
(liquid, filtered, PBS, trehalosle, protein G affinity purified) |
FC, IH, WB |
Human, Mouse, Rat |
Immunogen = Sf21-derived recombinant human Akt2 |
endogenous Akt2; not rhAkt1 or rhAkt3 |
- |
|
|
R&D Systems |
kit
|
Unknown Host
(capture antibody = anti-phospho-Akt1 (S473); detection antibody = biotinylated anti-phopho-Akt1 (S473)) |
ELISA |
Human, Mouse |
- |
measures natural and recombinant phospho-Akt (S473) |
- |
|
|
R&D Systems |
polyclonal
IgG |
Rabbit
(lyophilized, PBS, trehalose, affinity purified) |
WB |
Human, Mouse, Rat |
Immunogen = E. coli derived recombinant human Akt1 |
Akt1; not recombinant Akt2 or Akt3 in WB |
- |
|
|
R&D Systems |
monoclonal
IgG2a |
Mouse
(lyophilized, PBS, trehalose, protein G purified) |
FC |
Human |
Immunogen = purified, E. coli-derived full-length recombinant human Akt3 (aa1-479) |
Akt3; no cross reactivity with rhAkt1 or rhAkt2 |
- |
|
|
R&D Systems |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, protein G affinity purified, conj. PE) |
FC |
Human |
- |
Akt2 |
- |
|
|
R&D Systems |
monoclonal
IgG3 |
Mouse
(lyophilized, PBS, trehalose, protein G purified) |
WB |
Human, Mouse, Rat |
Immunogen = Sf21-derived recombinant human Akt2 |
rhAkt2 in ELISA and endogenous Akt2 in WB; not rhAkt1 or rhAkt3 |
- |
|
|
R&D Systems |
monoclonal
IgG1 |
Mouse
(lyophilized, PBS, trehalose, affinity purified) |
FC, IC, WB |
Human |
Immunogen = E. coli derived recombinant human Akt1 |
Akt1; not rhAkt2 or rhAkt3 |
- |
|
|
R&D Systems |
kit
|
Unknown Host
(capture antibody = phospho-Akt1 (S473); detection antibody = phospho-Akt1 (S473)) |
ELISA |
Human, Mouse |
- |
natural and recombinant phosphorylated Akt1 Ser473; minimal cross-reactivity with phosphorylated Akt2 and akt3 |
- |
|
|
R&D Systems |
kit
|
Unknown Host
(capture antibody = total Akt1; detection antibody = biotinylated total Akt1) |
ELISA |
Human, Mouse, Rat |
- |
total Akt1 |
- |
|
|
R&D Systems |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide; conj. Allophycocyanin (APC), FITC or phycoerythrin (PE)) |
FC |
Human |
Immunogen = E. coli derived recombinant human Akt1 |
Akt1 |
- |
|
|
R&D Systems |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, protein G affinity purified, conj. phycoerythrin (PE) or Allophycocyanin (APC)) |
FC |
Human |
- |
Akt2 |
- |
|
|
R&D Systems |
polyclonal
IgG |
Sheep
(lyophilized, PBS, trehalose, affinity purified) |
WB |
Human, Mouse, Rat |
Immunogen = E. coli derived recombinant human Akt1, aa1-149 |
Akt1; not rhAkt2 or rhAkt3 |
- |
|
|
R&D Systems |
polyclonal
IgG |
Goat
(lyophilized, PBS, trehalose, affinity purified) |
IH, WB |
Human, Mouse, Rat |
Immunogen = Sf21-derived recombinant human Akt2 |
endogenous Akt2 also rhAkt2; no reactivity with rhAkt1 or Akt3 |
- |
|
|
Upstate and Chemicon are Millipore |
monoclonal
IgG |
Mouse
(Tris-glycine, NaCl, azide, glycerol, Protein G purified) |
FC, IP, WB |
Human, Mouse, Rat |
Immunogen = GST-fusion protein corresponding to residues 1-149 of human Akt-1 |
Akt1/PKBα >Akt2/PKBβ >Akt3/PKBγ (weak to none) |
- |
|
|
Upstate and Chemicon are Millipore |
monoclonal
IgG |
Mouse
(frozen, PBS, Protein G purified) |
IP, WB |
Human, Mouse, Rat |
Immunogen = GST-fusion protein corresponding to aa residues 1-149 of human Akt1 |
Akt1/PKBα > Akt2/PKBβ (30%) > Akt3/PKBγ (weak to none) |
- |
|
|
Upstate and Chemicon are Millipore |
monoclonal
IgG |
Mouse
(liquid, PBS, azide, Protein G purified, agarose conj.) |
IP, WB |
Human, Rat |
Immunogen = GST-fusion protein corresponding to aa residues 1-149 of human Akt1 |
Akt1/PKBα > Akt2/PKBβ (30%) > Akt3/PKBγ (weak to none) |
- |
|
|
Upstate and Chemicon are Millipore |
monoclonal
IgG |
Rabbit
(liquid, tris-glycine, NaCl, azide, glycerol, Protein A purified) |
IP, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide corresponding to the C-terminal residues 466-480 of rat Akt1 |
Akt1/PKBα. Slight cross-reactivity with Akt2 |
- |
|
|
Upstate and Chemicon are Millipore |
monoclonal
IgG |
Mouse
(liquid, Protein G purified, PBS, Tween, azide, Alexa Fluor® 488 conjugate) |
FC, IC, IF, WB |
Human, Mouse, Rat |
Immunogen = GST-fusion protein corresponding to aa residues 1-149 of human Akt1 |
Akt1/PKBα > Akt2/PKBβ (30%) > Akt3/PKBγ (weak to none) |
- |
|
|
Upstate and Chemicon are Millipore |
monoclonal
IgG |
Mouse
(liquid, Protein G purified, PBS, Tween, azide, Alexa Fluor® 555 conjugate) |
FC, IC, WB |
Human, Mouse, Rat |
Immunogen = GST-fusion protein corresponding to aa residues 1-149 of human Akt1 |
Akt1/PKBα > Akt2/PKBβ (30%) > Akt3/PKBγ (weak to none) |
- |
|
|
Upstate and Chemicon are Millipore |
polyclonal
IgG |
Rabbit
(liquid, tris-glycine, NaCl, azide, glycerol, Protein A purified) |
IP, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide corresponding to the C-terminal residues 466-480 of rat Akt1 |
Akt1/PKBα |
- |
|
|
Upstate and Chemicon are Millipore |
polyclonal
IgG |
Rabbit
(liquid, tris-glycine, NaCl, azide, glycerol, affinity purified) |
IP, WB |
Human, Mouse |
Immunogen = synthetic peptide corresponding to aa 88-199 of human Akt1/PKBα Pleckstrin Homology Domain |
Akt1/PKBα |
- |
|
|
Upstate and Chemicon are Millipore |
kit
|
Unknown Host
(capture ab = monoclonal mAkt-1; detection ab = rabbit anti-Akt1) |
ELISA |
Human, Mouse, Rat |
- |
Akt1 |
- |
|
|
Upstate and Chemicon are Millipore |
kit
|
Unknown Host
(anti-Akt/PKB clone SKB1 Cat# 05-591; radioactive) |
IP |
- |
- |
Akt/PKB: Akt1/PKBα |
- |
|
|
Upstate and Chemicon are Millipore |
kit
|
Rabbit
(KinEASE™ FP Fluorescein Green Assay; STK ab Cat#35-002) |
- |
- |
- |
Akt1/PKBα |
- |
|
|
Upstate and Chemicon are Millipore |
kit
|
Rabbit
(KinEASE™ FP-645nm FarRed Assay; STK ab Cat#35-002) |
- |
- |
- |
Akt1/PKBα |
- |
|
|
Upstate and Chemicon are Millipore |
monoclonal
IgG |
Rabbit
(liquid, tris-glycine, NaCl, glycerol, azide, protein A purified) |
IP, WB |
Human, Rat |
Immunogen = synthetic peptide corresp. to aa 455-469 of human Akt2/PKBβ |
Akt2/PKBβ |
- |
|
|
Upstate and Chemicon are Millipore |
polyclonal
IgG |
Rabbit
(liquid, tris-glycine, NaCl, glycerol, azide, Protein A purified) |
IP, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide corresp. to aa 455-469 of human Akt2/PKBβ |
Akt2/PKBβDoes not cross react with Akt1 or Akt3 |
- |
|
|
Upstate and Chemicon are Millipore |
kit
|
Unknown Host
(KinEASE™ FP Fluorescein Green Assay; STK ab Cat#35-002) |
- |
- |
- |
Akt2/PKBβ |
- |
|
|
Upstate and Chemicon are Millipore |
kit
|
Unknown Host
(KinEASE™ FP-645nm FarRed Assay; STK ab Cat#35-002) |
- |
- |
- |
Akt2/PKBβ |
- |
|
|
Upstate and Chemicon are Millipore |
monoclonal
IgG2a |
Mouse
(liquid, tris-glycine, NaCl, glycerol, azide, Protein A purified) |
IP, WB |
Human |
Immunogen = synthetic peptide corresp. to aa 119-131 of human Akt3/PKBγ |
Akt3/PKBγnot Akt1 or Akt2 |
- |
|
|
Upstate and Chemicon are Millipore |
polyclonal
IgG |
Rabbit
(liquid, tris-glycine, NaCl, glycerol, azide, Protein A purified) |
IP, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide corresp. to aa 119-131 of human Akt3/PKBγ |
Akt3/PKBγ |
- |
|
|
Upstate and Chemicon are Millipore |
kit
|
Unknown Host
(KinEASE™ FP Fluorescein Green Assay; STK ab Cat#35-002) |
- |
- |
- |
Akt3/PKBγ |
- |
|
|
Upstate and Chemicon are Millipore |
kit
|
Unknown Host
(KinEASE™ FP-645nm FarRed Assay; STK ab Cat#35-002) |
- |
- |
- |
Akt3/PKBγ |
- |
|
|
Rockland |
polyclonal
|
Sheep
(liquid, sterile filtered, KPBS, azide, affinity purified) |
ELISA, IF, IH, IP, WB |
Chicken, Human, Mouse, Rat |
Immunogen = synthetic peptide corresp. to the internal region of Akt1 protein |
Akt (55-60kDa) |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, gelatin, azide) |
ELISA, FC, IF, IP, WB |
Human, Mouse, Rat |
Immunogen = aa345-480 of human Akt1 |
Akt1, and to a lesser extent, Akt2 and Akt3 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
Immunogen = short aa sequence containing phosphorylated Ser473 of human Akt1 |
Akt1/2/3 phosphorylated at Ser473 |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, gelatin, azide, available as agarose for IP) |
IF, IH, IP, WB |
Human |
Immunogen = peptide mapping at the C-terminus of human Akt2 |
Akt2 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, gelatin, azide, affinity pure) |
IF, IP, WB |
Human, Mouse, Rat |
Immunogen = peptide mapping near the C-terminus of human Akt3 |
Akt3 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, gelatin, azide, affinity pure) |
IF, IP, WB |
Human, Mouse, Rat |
Immunogen = peptide mapping near the C-terminus of mouse Akt3 |
Akt3 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, gelatin, azide, affinity purified) |
FC, IF, IP, WB |
Mammal (various), Cow, Human, Mouse, Rat, Xenopus |
Immunogen = peptide mapping at the C-terminus of human Akt1 |
Akt1, and to a lesser extent, Akt2 and Akt3 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, gelatin, azide, affinity purified) |
IF, IP, WB |
Mammal (various), Cow, Human, Mouse, Rat |
Immunogen = peptide mapping near the C-terminus of human Akt1 |
Akt1; no cross-reactivity with Akt2 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
IF, IP, WB |
Human, Mouse, Rat, Xenopus |
Immunogen = aa345-480 of human Akt1/2 |
Akt1, Akt2 and Akt3 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
ELISA, IF, IH, IP, WB |
Human, Mouse, Rat |
Immunogen = short aa seq. containing phosphorylated Thr308 of human Akt2 |
Akt1/2/3 phosphorylated at Thr308 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, gelatin, azide, affinity pure) |
IF, WB |
Mammal (various), Dog, Human, Mouse, Rat |
Immunogen = peptide mapping at the C-terminus of human Akt2 |
Akt2; no cross-reactivity with Akt1 |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, gelatin, azide, PEG, sucrose) |
WB |
Human, Mouse, Rat |
Immunogen = aa466-480 of human Akt1 |
Akt1 |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, gelatin, azide, PEG, sucrose) |
IF, WB |
Human, Rat |
Immunogen = aa107-122 of human Akt1 |
Akt1 |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, gelatin, azide, PEG, sucrose) |
WB |
Human |
Immunogen = synthetic peptide of human Akt1 |
Akt1; may cross-react with Akt2 and Akt3 |
- |
|
|
Santa Cruz |
monoclonal
IgM |
Mouse
(liquid, PBS, gelatin, azide, PEG, sucrose) |
WB |
Human |
Immunogen = peptide corresp. to the activation loop of of human Akt1 |
Akt1 |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, gelatin, azide, PEG, sucrose) |
WB |
Human |
Immunogen = synthetic peptide of human Akt1 |
Akt1; may cross-react with Akt2 and Akt3 |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
Immunogen = aa345-480 of human Akt1 |
Akt1 |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
WB |
Human, Mouse, Rat, Xenopus |
Immunogen = aa461-477 of human Akt1 |
Akt1 |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, gelatin, azide, PEG, sucrose) |
WB |
Human, Mouse, Rat |
Immunogen = synthetic phospho-peptide corresp. to aa residues surrounding Serine 473 of human Akt1 |
Akt1 phosphorylated at Ser473 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
IF, IH, IP, WB |
Human, Mouse, Rat |
Immunogen = peptide corresp. to phosphorylated Ser473 of human Akt1 |
Akt1 phosphorylated at Ser473 and corresp. to phosphorylated Akt2 and Akt3 |
- |