|
|
AnaSpec |
polyclonal
|
Rabbit
(liquid PBS, azide, affinity purified) |
ELISA, WB |
Human |
Immunogen = synthetic peptide corresponding to the C-terminus of human AKT2/PBK β (differs from corresponding mouse and rat sequence by a single amino acid) |
AKT2 |
- |
|
|
AnaSpec |
polyclonal
|
Rabbit
(liquid PBS, azide, affinity purified) |
WB |
Human, Mouse |
Immunogen = synthetic phosphopeptide corresponding to human AKT1 at the phosphorylation site of threonine 308 |
detects AKT |
- |
|
|
AnaSpec |
polyclonal
|
Rabbit
(liquid immunoaffinity purified IgG, 50 μg in 250 μl of 1X PBS containing 0.05% sodium azide) |
WB |
Human, Mouse |
Immunogen = synthetic phosphopeptide derived from the region of human AKT that contains serine 473 (PQFpSYSA) |
detects AKT1 phos Ser473 |
- |
|
|
AnaSpec |
polyclonal
IgG |
Rabbit
(liquid, MOPS, BSA, glycerol, affinity purified) |
IH, WB |
Zebra fish |
Immunogen = synthetic peptide derived from the intermediate region of zebrafish Akt3 (Genebank XP_00192252) |
Akt3 |
- |
|
|
AnaSpec |
polyclonal
IgG |
Rabbit
(liquid, MOPS, BSA, glycerol, affinity purified) |
ELISA, IH, WB |
Zebra fish |
Immunogen = synthetic peptide derived from the C-terminus of zebrafish Akt3 (Genebank XP_00192252) |
Akt3 |
- |
|
|
AnaSpec |
polyclonal
|
Rabbit
(liquid PBS, azide, affinity purified) |
ELISA, WB |
Human, Mouse |
Immunogen = synthetic peptide corresponding to the N-terminus of AKT3/PBK γ |
detects Akt3 |
- |
|
|
Cell Signaling |
monoclonal
IgG2a |
Mouse
(liquid, HEPES, NaCl, BSA, glycerol, azide) |
IP |
Human, Hamster, Mouse, Rat |
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of human Akt |
binds preferentially to Akt phosphorylated at Ser473; also binds to some non-phospho-Akt |
- |
|
|
Cell Signaling |
kit
|
Rabbit
(includes: Phospho-Akt (Thr308) (C31E5E) Rabbit mAb #2965 and Akt (pan) (C67E7) Rabbit mAb #4691) |
FC, IC, IF, IH, WB |
Human, Monkey, Hamster, Mouse, Rat, Drosophila |
- |
assess protein activation status |
- |
|
|
Cell Signaling |
kit
|
Rabbit
(includes: Phospho-Akt (Thr308) (C31E5E) Rabbit mAb # 2965, Phospho-Akt (Ser473) (D9E) XP™ Rabbit mAb # 4060 and Akt (pan) (C67E7) Rabbit mAb # 4691) |
FC, WB |
Dog, Chicken, Zebra fish, Human, Hamster, Mouse, Rat, Xenopus, Drosophila |
immunogen = synthetic phospho-peptide (KLH-coupled) corresp. to residues surrounding either Ser473, Thr308 or from the C-terminal sequence of mouse Akt |
detect endogenous levels of Akt only when phosphorylated at either Ser473 or Thr308; Also detects endogenous levels of total Akt1/2/3 independent of their phosphorylation state; no cross-reaction with related kinases. |
- |
|
|
Cell Signaling |
monoclonal
IgG2b |
Mouse
(liquid, HEPES, NaCl, BSA, glycerol, azide) |
IP, WB |
Human, Hamster, Mouse, Rat |
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt |
reacts with endogenous Akt only when phosphorylated at Ser473; no detection of Akt phosphorylated at other sites or related kinases such as PKC and p70 S6 kinase |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide, biotinylated) |
FC, WB |
Bovine, Zebra fish, Human, Hamster, Mouse, Rat, Drosophila |
immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of humanAkt (KLH-coupled) |
reacts with endogenous Akt only when phosphorylated at Ser473 |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(HEPES, NaCl, BSA, glycerol, azide) |
FC, IC, IF, IP, WB |
Human, Mouse, Rat |
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt |
reacts with endogenous levels of Akt only when phosphorylated at Ser473 |
- |
|
|
Cell Signaling |
kit
|
Rabbit
(HEPES, NaCl, BSA, glycerol, Protein A and affinity purified) |
WB |
Chicken, Human, Hamster, Mouse, Rat |
Akt pS473 (#9271) = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt.Akt (#9272) = synthetic peptide derived from the C-terminal seq of mouse Akt. |
detects Akt1 when phosphorylated at Ser473 and Akt2 and Akt3 when phosphorylated at equivalent sites.Akt detects total Akt1, Akt2 and Akt3. |
- |
|
|
Cell Signaling |
kit
|
Rabbit
(ready-to-use, Protein A and affinity purified) |
IH |
Human |
AktpSer473 (#9277) = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt |
detects Akt1 when phosphorylated at Ser473 and Akt2 and Akt3 when phosphorylated at equivalent sites. |
- |
|
|
Cell Signaling |
kit
|
Rabbit
(multi antibody sampler kit; HEPES, saline, BSA, glycerol, azide) |
IC, IF, IH, IP, WB |
Chicken, Human, Monkey, Mouse, Rat |
|
various antibodies for phospho.Akt pathway: phospho Akt,pan Akt,phospho c-Raf,phospho GSK-3β,phospho PTEN,phospho PDK1 |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(HEPES, NaCl, BSA, glycerol, azide) |
IP, WB |
Human, Mouse, Rat |
Immunogen = synthetic phospho-peptide (KLH-coupled) corresp. to residues aroundThr308 of mouse Akt. |
reacts with Akt phosphor. at Thr308 only |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(HEPES, NaCl, BSA, glycerol, azide, biotinylated) |
IP, WB |
Human |
Immunogen = synthetic phospho-peptide (KLH-coupled) corresp. to residues aroundThr308 of mouse Akt |
reacts with Akt phosphorylated at Thr308 only |
- |
|
|
Cell Signaling |
monoclonal
IgG1 |
Mouse
(liquid, HEPES, NaCl, BSA, glycerol, azide) |
WB |
Human, Monkey, Mouse, Rat |
Immunogen = synthetic phospho-peptide corresp. to residues aroundThr308 of mouse Akt (KLH-coupled) |
reacts with endogenous levels of Akt only when phosphorylated at Thr308 |
- |
|
|
Cell Signaling |
polyclonal
|
Rabbit
(HEPES, NaCl, BSA, glycerol, Protein A and affinity purified) |
FC, IC, IF, IH, IP, WB |
Human, Hamster, Mouse, Rat |
Immunogen = synthetic phopho-peptide corresp. to residues around Thr308 of mouse Akt |
reacts with Akt phos Thr308 only. Does not detect Akt phosphorylated at other sites or related kinases |
- |
|
|
Cell Signaling |
kit
|
Unknown Host
(capture ab = Akt rabbit #9272; detection ab = monoclonal Akt pThr308 #5103 PathScan®) |
ELISA |
Human, Mouse |
- |
detects Akt when phosphorylated at Thr308 |
- |
|
|
Cell Signaling |
polyclonal
|
Rabbit
(HEPES, NaCl, BSA, glycerol, Protein A and affinity purified) |
IP, WB |
Human, Mouse, Rat |
Immunogen = synthetic phopho-peptide corresp. to residues around Thr451 of Akt2 |
reacts with endogenous levels of Akt1 only when phosphorylated at Thr450; also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues; not Akt phosphorylated at other sites |
- |
|
|
Cell Signaling |
polyclonal
|
Rabbit
(HEPES, NaCl, BSA, glycerol, azide, protein A/affinity purified) |
WB |
Human, Mouse, Rat |
Immunogen = synthetic phospho-peptide corresp. to residues surrounding Tyr326 of Akt (KLH coupled) |
reacts with Akt only when phosphorylated at Tyr326; no cross-reaction with Akt phosphorylated at other sites |
- |
|
|
Cell Signaling |
kit
|
Rabbit
(includes: Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb #4858 and Akt (pan) (C67E7) Rabbit mAb #4691PTEN (D4.3) Rabbit mAb #9188) |
IH |
Human, Mouse |
immunogen = synthetic phospho-peptides corresp. to residues surrounding the phosphorylation site(s) of interest, and synthetic peptides derived from the C-terminal seq. of mouse Akt or from the C-terminal seq. of human PTEN |
paraffin-embedded tissues or cells with antibodies directed against proteins involved in Akt signaling |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, conj. AlexaFluor®488) |
FC |
Human |
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt |
detects endogenous levels of Akt only when phosphorylated at Ser473 |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, conj. AlexaFluor®647) |
FC |
Human |
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt |
detects endogenous levels of Akt only when phosphorylated at Ser473 |
- |
|
|
Cell Signaling |
monoclonal
IgG2b |
Mouse
(liquid, HEPES, NaCl, BSA, glycerol, azide, biotinylated) |
IP |
Human, Hamster, Mouse, Rat |
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt |
detects endogenous Akt only when phosphorylated at Ser473; no detection of Akt phosphorylated at other sites or related kinases such as PKC and p70 S6 kinase |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide) |
IH |
Human, Mouse, Rat |
immunogen = synthetic phospho-peptide corresp. to residues surrounding Ser473 of mouse Akt (KLH-coupled) |
detects Akt only when phosphorylated at Ser473 and Akt2 and Akt3 only when phosphorylated at equivalent sites |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, conj. AlexaFluor®488) |
FC |
Human, Mouse, Rat |
immunogen = synthetic phospho-peptide corresp. to residues surrounding Ser473 of humanAkt (KLH-coupled) |
detects Akt only when phosphorylated at Ser473 |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, conj. AlexaFluor®647) |
FC |
Human, Mouse, Rat |
immunogen = synthetic phospho-peptide corresp. to residues surrounding Ser473 of humanAkt (KLH-coupled) |
detects Akt only when phosphorylated at Ser473 |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide) |
IP |
Bovine, Dog, Chicken, Zebra fish, Human, Hamster, Mouse, Rat, Drosophila |
immunogen = synthetic phospho-peptide corresp. to residues surrounding Ser473 of humanAkt (KLH-coupled) |
detects endogenous levels of Akt only when phosphorylated at Ser473 |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide) |
IH |
Human, Mouse, Rat |
Immunogen = synthetic phospho-peptide corresp. to residues aroundThr308 of mouse Akt (KLH-coupled) |
detects endogenous levels of Akt only when phosphorylated at Thr308 |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide) |
FC, IC, IF, WB |
Human, Monkey, Mouse, Rat |
Immunogen = synthetic phospho-peptide corresp. to residues aroundThr308 of mouse Akt (KLH-coupled) |
reacts with endogenous levels of Akt only when phosphorylated at Thr308 |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide; conj. AlexaFluor®488) |
FC |
Human, Monkey, Mouse, Rat |
Immunogen = synthetic phospho-peptide corresp. to residues aroundThr308 of mouse Akt (KLH-coupled) |
detects endogenous levels of Akt only when phosphorylated at Thr308 |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide; conj. AlexaFluor®647) |
FC |
Human, Monkey, Mouse, Rat |
Immunogen = synthetic phospho-peptide corresp. to residues aroundThr308 of mouse Akt (KLH-coupled) |
detects endogenous levels of Akt only when phosphorylated at Thr308 |
- |
|
|
Biodesign Meridian LifeScience |
monoclonal
IgG1 |
Mouse
(lyophilized, supernatant, PBS, PEG, sucrose, azide, purified) |
ELISA, WB |
Dog, Human, Mouse, Rat |
Immunogen = synthetic peptide corresp. to C-terminus (aa 466-480) coupled to KLH |
AKT/PKB |
- |
|
|
Biodesign Meridian LifeScience |
polyclonal
|
Rabbit
(liquid, serum, KPS, azide) |
ELISA, IF, IH, IP, WB |
Chicken, Human, Mouse, Rat |
Immunogen = synthetic peptide (C R P H F P Q F S Y S A S G T A) corresp. to the C-terminus (aa 460-480) of human, rat, mouse and chicken AKT proteins coupled to KLH |
AKT |
- |
|
|
Biodesign Meridian LifeScience |
polyclonal
|
Rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
IH, WB |
Human, Mouse, Rat |
Immunogen = synthetic non-phosphopeptide derived from human Akt around the phosphorylation site of threonine 308 (M-K-Tp-F-C) |
detects total Akt protein (aa 306-310) |
- |
|
|
Biodesign Meridian LifeScience |
polyclonal
|
Rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
IH, WB |
Human, Mouse, Rat |
Immunogen = synthetic non-phosphopeptide derived from human Akt around the phosphorylation site of serine 473 (Q-F-Sp-Y-S) |
detects total Akt protein (aa 471-475) |
- |
|
|
Biodesign Meridian LifeScience |
polyclonal
|
Rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
IH, WB |
Human |
Immunogen = synthetic phosphopeptide derived from human Akt around the phosphorylation site of serine 473 (Q-F-Sp-Y-S) |
detects Akt only when phosphorylated at Ser473 |
- |
|
|
Biodesign Meridian LifeScience |
polyclonal
|
Rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
IH, WB |
Human, Mouse, Rat |
Immunogen = synthetic phosphopeptide derived from human Akt around the phosphorylation site of threonine 308 (M-K-Tp-F-C) |
detects Akt only when phosphorylated at Thr308 |
- |
|
|
Biodesign Meridian LifeScience |
polyclonal
|
Rabbit
(liquid, PBS, azide, affinity purified) |
ELISA, IF, IH, WB |
Human |
Immunogen = synthetic peptide corresponding to the C-terminus aa 460-480 of human, mouse, rat, chicken AKT proteins coupled to KLH |
AKT phosphorylated Ser473. Minimal reactivity against non-phosphorylated AKT |
- |
|
|
Biodesign Meridian LifeScience |
polyclonal
|
Rabbit
(liquid, PBS, azide, affinity purified) |
ELISA, WB |
Human |
Immunogen = synthetic peptide corresponding to aa 302-311 of human AKT protein coupled to KLH |
AKT phosphorylated T308. Minimal reactivity against non-phosphorylated AKT |
- |
|
|
Biodesign Meridian LifeScience |
polyclonal
|
Rabbit
(liquid,affinity purified, PBS, azide) |
ELISA, WB |
Human |
Immunogen = synthetic peptide corresponding to aa 455-468 of human AKT2 protein coupled to KLH |
AKT2 (phosphorylated and unphosphorylated) |
- |
|
|
Biodesign Meridian LifeScience |
polyclonal
|
Rabbit
(liquid,affinity purified, PBS, azide) |
IH, WB |
Human, Mouse, Rat |
Immunogen = synthetic non-phosphopeptide derived from human Akt2 around the phosphorylation site of serine 474 (Q-F-Sp-Y-S) |
detects total Akt2 protein (amino acids 472-476) |
- |
|
|
Biodesign Meridian LifeScience |
polyclonal
|
Rabbit
(liquid,affinity purified, PBS, azide) |
IH, WB |
- |
Immunogen = synthetic phosphopeptide derived from human Akt2 around the phosphorylation site of serine 474 (Q-F-Sp-Y-S) |
detects AKT2 only when phosphorylated at serine 474 |
- |
|
|
Cell Signaling |
polyclonal
|
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, Protein A and affinity purified) |
FC, IC, IF, IP, WB |
Bovine, Dog, Pig, Human, Hamster, Mouse, Rat, Drosophila |
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt |
reacts with Akt when phosphorylated at Ser473; also reacts with Akt2 and Akt3 when phosphorylated at corresponding residues. Does not recognize Akt phosphorylated at other sites, nor does it recognize phosphorylated forms of related kinases such as PKC or p70 S6 kinase |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide) |
FC, IC, IF, IH, IP, WB |
Bovine, Zebra fish, Human, Monkey, Hamster, Mouse, Rat, Drosophila |
immunogen = synthetic phospho-peptide corresp. to residues surrounding Ser473 of humanAkt |
reacts with Akt only when phosphorylated at Ser473 |
- |
|
|
AbD Serotec |
monoclonal
Fab bivalent |
Mouse
(purified) |
ELISA, WB |
Human |
Immunogen = human Akt1 (val106-Ala480, MW 77.8kDa) fusion protein; contains the complete kinase domain (aa 150 to 408) but not the N-terminal PH domain |
- |
- |
|
|
Biotrend |
polyclonal
IgG |
Rabbit
(liquid, IgG fraction, affinity purified) |
IF, IH |
Human, Mouse, Rat |
- |
- |
- |
|
|
Stressgen Enzo Life Sciences |
polyclonal
|
Rabbit
(liquid, PBS, glycerol, azide, protein A purified) |
WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide derived from the sequence of human Akt aa 88-100 |
recognizes Akt/PKB (~59kDa); detects both phosphorylated and unphosphorylated PKB |
- |
|
|
Stressgen Enzo Life Sciences |
polyclonal
Ig |
Rabbit
(liquid, Dulbecco's PBS, glycerol, BSA, azide, affinity purified) |
IF, WB |
Human, Mouse |
Immunogen = phospho-peptide sequence conserved between mouse and rat Akt1 and Akt2 |
Akt1 phosphorylated at Thr308. No reactivity with non-phosphorylated Akt/PKB |
- |
|
|
Stressgen Enzo Life Sciences |
polyclonal
Ig |
Rabbit
(liquid, tris-acetate, azide, affinity purified) |
ELISA, WB |
Bovine, Dog, Rabbit, Chicken, Sheep, Pig, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat, Xenopus |
Immunogen = synthetic peptide (A S I R E), derived from seq. near the C-terminus of human Akt2 aa 477-481, conjugated to KLH |
Akt2/PKBβ(~65kDa). Not PKBα (Akt1) or PKBγ (Akt3) |
- |
|
|
Stressgen Enzo Life Sciences |
polyclonal
Ig |
Rabbit
(liquid, affinity purified) |
WB |
Bovine, Chicken, Human, Mouse, Rat |
Immunogen = synthetic phospho-peptide derived from the C-terminus of Akt |
Akt1, Akt2, Akt 3 phosphorylated at Ser473. No reactivity with non-phosphorylated Akt/PKB |
- |
|
|
Stressgen Enzo Life Sciences |
polyclonal
|
Rabbit
(liquid, PBS, azide, glycerol, protein A affinity purified) |
WB |
Human, Mouse, Rat |
immunogen = synthetic peptide derived from the C-terminus of human Akt |
recognizes Akt1/2 (~56kDa) |
- |
|
|
Stressgen Enzo Life Sciences |
kit
|
(colorimetric kit: microtiter plate, conjugate, antibody, assay buffer 32, wash buffer concentrate, standard, TMB substrate, cell lysis buffer 5, stop solution 2) |
ELISA |
Human, Mouse, Rat |
- |
detects Akt1/2 phospho-Ser473; recognizes Akt3 with low affinity |
- |
|
|
Stressgen Enzo Life Sciences |
kit
|
(non-radioactive Akt kinase activity assay: antibody, microtiter plate, conjugate, antibody dilution buffer, wash buffer concentrate, TMB substrate, stop solution 2) |
ELISA |
Non-specific |
- |
measurement of Akt in partially purified, purified, or crude enzyme preparations from any species |
- |
|
|
Alexis Enzo Life Sciences |
monoclonal
IgG1 |
Mouse
(liquid, PBS, glycerol, azide, PEG, sucrose) |
IH, WB |
Dog, Human, Rat |
Immunogen = synthetic peptide corresp. to human Akt1 (Protein Kinase Bα; PKBα) |
Akt1/PKBα |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(liquid, ascites) |
ELISA, WB |
Human |
immunogen = Ni-NTA purified truncated recombinant Akt3 expressed in E. coli strain BL21 (DE3) |
Akt3 |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(liquid, ascites) |
ELISA, WB |
Human |
immunogen = Ni-NTA purified truncated recombinant Akt3 expressed in E. coli strain BL21 (DE3) |
Akt3 |
- |
|
|
Alexis Enzo Life Sciences |
monoclonal
IgG2a |
Mouse
(lyophilized, PBS, glycerol, azide, PEG, sucrose, purified) |
IH, WB |
Human |
Immunogen = synthetic peptide corresp. to human Akt2 |
Akt2 |
- |
|
|
Alexis Enzo Life Sciences |
monoclonal
IgG1 |
Mouse
(lyophilized supernatant, azide, PEG, sucrose, purified) |
WB |
Dog, Human, Mouse, Rat |
Immunogen = synthetic peptide corresp. to C-terminal (PQFpSYSA) of human Akt (protein kinase B; PKB) phosphorylated at Ser473 |
Ser473-phosphorylated Akt; no cross-reaction with dephosphorylated Akt or unrelated serine-phosphorylated proteins. |
- |
|
|
Alexis Enzo Life Sciences |
monoclonal
IgG1 |
Mouse
(liquid PBS, BSA, azide, Protein G purified) |
WB |
Human |
Immunogen = synthetic peptide corresp. to aa 119-136 of human Akt3. |
detects Akt3, ~50 kDa by WB |
- |
|
|
Alexis Enzo Life Sciences |
monoclonal
IgG1 |
Mouse
(lyophilized supernatant, azide, PEG, sucrose, purified) |
WB |
Dog, Human, Mouse, Rat |
Immunogen = synthetic peptide corresp. to C-terminal human Akt |
recognizes a c-terminal epitope (aa 466-480) of Akt/PKB (phosphorylated and non-phosphorylated) |
- |
|
|
Alexis Enzo Life Sciences |
polyclonal
|
Rabbit
(liquid PBS, BSA, azide, affinity purified) |
WB |
Mouse |
Immunogen = synthetic phospho-peptide corresp. to aa 302-314 of mouse Akt (PKB) |
Akt/PBK (phosphorylated and non-phosphorylated) |
- |
|
|
Alexis Enzo Life Sciences |
polyclonal
|
Rabbit
(liquid, neat serum, azide) |
ELISA, IP, WB |
Mouse |
Immunogen = recombinant mouse Akt |
Recognizes mouse Akt; no cross with other species |
- |
|
|
Alexis Enzo Life Sciences |
monoclonal
IgG1 |
Mouse
(lyophilized, PBS, azide, PEG, sucrose, purified) |
IH, WB |
Dog, Human, Mouse, Rat |
Immunogen = synthetic peptide corresp. to C-terminal (QFpSYS A) of human Akt (protein Kinase B; PKB) dephosphorylated at Ser472/473/474 |
Akt dephosphorylated at Ser473; no cross-reaction with akt phosphorylated at Ser473 |
- |
|
|
Alexis Enzo Life Sciences |
monoclonal
IgG1 |
Mouse
(liquid, PBS, BSA, azide, protein A purified) |
ELISA, IP, WB |
Dog, Human, Hamster, Mouse, Rat |
Immunogen = recombinant mouse Akt (protein kinase B; PKB) |
Detects Akt1 (PKB-α) and Akt2 (PKB-β) strong than Akt3 (PKB-γ). Detects also phosphorylated forms of Akt |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(lyophilized, purified) |
ELISA, WB |
Dog, Human, Mouse, Rat |
immunogen = synthetic peptide sequence (aa466-480) located at the C-terminus of Akt (KLH coupled) |
Akt1 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, gelatin, azide) |
ChIP, IF, IH, IP, WB |
Human, Mouse, Rat, Xenopus |
Immunogen = peptide mapping at the N-terminus of human Akt1 |
Akt1 and Akt2 |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol) |
WB |
Human, Mouse, Rat |
Immunogen = synthetic phospho-peptide corresp. to residues around Thr389 of human p70 S6 kinase |
detects endogenous levels of p70 S6 kinase only when phosphorylated at threonine 389; also detects p85 S6 kinase when phosphorylated at the analogous site (Thr412), and possibly S6KII phosphorylated at Thr401 |
- |
|
|
Cell Signaling |
polyclonal
|
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide) |
WB |
Human, Monkey, Mouse, Rat |
Immunogen = synthetic phospho-peptide corresp. to residues around Thr389 of human p70 S6 kinase |
detects endogenous levels of p70 S6 kinase only when phosphorylated at threonine 389; also detects p85 S6 kinase when phosphorylated at the analogous site (Thr412), and possibly S6KII phosphorylated at Thr401 |
- |
|
|
None |
monoclonal
IgM, κ |
Mouse
(liquid, PBS, azide, affinity purified) |
IF, WB |
Human |
Immunogen = peptide corresp. to the activation loop of Akt |
Akt/PKBα |
discontinued |
|
|
None |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, glycerol, affinity purified) |
IF, WB |
Human |
Immunogen = recombinant (partial), C-terminal human Akt |
Akt |
discontinued |
|
|
None |
polyclonal
|
Rabbit
(PBS, no preservative, affinity purified) |
WB |
Human, Mouse |
Immunogen = synthetic phospho-peptide derived from a region of PKB/Akt containing threonine 308 |
Akt/PKB containing threonine308 (65kDa) |
Discontinued |
|
|
None |
monoclonal
|
Rabbit
(liquid, PBS, glycerol, BSA, azide, protein A purified) |
IH, WB |
Human, Mouse |
Immunogen = synthetic phospho-peptide derived from region of human Akt containing serine 473. |
Akt phosphorylated at Ser473 (65kDa) |
Discontinued |