|
|
Autogen Bioclear |
monoclonal
IgG1 |
Mouse
(lyophilized, supernatant, PBS, azide, sucrose) |
ELISA, WB |
Dog, Human, Mouse |
immunogen = peptide corresp. to C-terminus aa 466-480 conj. to KLH |
Akt |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, KPBS, azide, affinity purified) |
ELISA, IH, WB |
Chicken, Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to C-terminus aa 460-480 of human, mouse, rat and chicken AKT proteins conj. to KLH |
Akt1 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, glycerol, azide, affinity purified) |
ELISA, IF, IH, WB |
Human |
Immunogen = synthetic peptide: C H F P Q F Sp Y S A S corresp. to aa460-480 of human AKT, conjugated to KLH |
Akt when phosphorylated at Ser473; minimal reactivity against non-phosphorylated AKT |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, proclin, affinity purified) |
ELISA, IP, WB |
Human |
Immunogen = synthetic peptide corresp. to human AKT1 phosphorylated at serine 473 |
Akt pSer473 (60kDa) |
- |
|
|
Autogen Bioclear |
monoclonal
IgG1 |
Mouse
(lyophilized, supernatant, PBS, azide, sucrose) |
ELISA, FC, IH, WB |
Dog, Human, Mouse, Rat |
immunogen = phosphopeptide Ser473 (P Q F Sp Y S A) conj. to KLH |
Akt phosphorylated at Ser473 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG1 |
Mouse
(lyophilized, supernatant, PBS, azide, sucrose, biotinylated) |
ELISA, IH, WB |
Dog, Human, Mouse, Rat |
immunogen = phosphopeptide Ser473 (P Q F Sp Y S A) conj. to KLH |
Akt phosphorylated at Ser473 |
- |
|
|
Autogen Bioclear |
polyclonal
|
Rabbit
(liquid, PBS, azide, affinity purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = 16 aa synthetic peptide from near the N-terminus of human Akt1 |
Akt1 |
- |
|
|
Autogen Bioclear |
monoclonal
IgM |
Mouse
(liquid, ascites, PBS, gelatin, azide) |
IH, WB |
Human |
immunogen = synthetic peptide V A N S L K Q R A P G E D P M D Y K (aa106-123) of human Akt2 |
Akt2 |
- |
|
|
Autogen Bioclear |
polyclonal
|
Rabbit
(liquid, PBS, azide) |
IH, IP, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to C-terminus of human AKT2 |
Akt2 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(lliquid, PBS, glycerol, azide, affinity purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide aa surrounding serine 474 of human AKT2 |
Akt2 phos Ser474 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(lliquid, PBS, glycerol, azide, affinity purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide aa surrounding serine 474 of human AKT2 (Q F Sp Y S) |
endogenous levels of Akt2 only when phosphorylated at Ser474 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(lliquid, PBS, glycerol, azide, affinity purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthesized non-phosphopeptide derived from human Akt2 around the phosphorylation site of serine 474 (Q-F-Sp-Y-S) |
endogenous levels of total Akt2 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, BSA, protein G purified) |
IH, WB |
Dog, Chicken, Cow, Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to aa residues 119-136 (C S P T S Q I D N I G E E E M D A S) of human Akt3, GenBank no. gi/ 4574744/gb/ AAD24196.1/ AF135794_1 |
Akt3 |
- |
|
|
Autogen Bioclear |
polyclonal
|
Rabbit
(liquid, PBS, azide) |
IH, WB |
Human, Mouse |
immunogen = synthetic peptide corresp. to the N-terminus of human Akt3 |
Akt3 |
- |
|
|
Autogen Bioclear |
polyclonal
|
Rabbit
(liquid, PBS, azide, affinity purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = 16 aa synthetic peptide located near the human Akt1 N-terminus |
Akt1 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG1 |
Mouse
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
ELISA, FC, IC, IF, IP |
Human, Hamster, Mouse, Rat |
immunogen = recombinant protein corresp. to aa140-480 of human Akt1 |
endogenous levels of Akt1; no crossreaction with other related proteins |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, glycerol, affinity purified) |
IF, WB |
Human |
immunogen = synthesized non-phosphopeptide derived from human Akt around the phosphorylation site of threonine 308 (M-K-Tp-F-C) |
endogenous levels of Akt1 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
IH, WB |
Human |
immunogen = synthesized non-phosphopeptide derived from human Akt2 around the phosphorylation site of serine 474 (Q-F-Sp-Y-S) |
Akt2 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS) |
WB |
Human, Mouse, Rat |
immunogen = synthetic peptide 16aa peptide located near the human Akt1 N-terminus |
Akt1 (48kDa) |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, KPBS, azide, affinity purified) |
ELISA, WB |
Human |
Immunogen = synthetic peptide corresp. to aa 302-311 of Akt (D G A T M K Tp F C G) |
Akt phosphorylated at T308 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG |
Human
(liquid, PBS, BSA, azide, glycerol, protein G purified) |
ELISA, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide derived from aa sequence 464-477 of human Akt1 |
Akt1 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide, purified) |
IH, WB |
Mouse, Rat |
immunogen = synthetic peptide |
Akt1 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide, purified) |
WB |
Human, Mouse, Rat |
immunogen = synthetic peptide |
Akt1 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide, purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide |
Akt2 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
|
WB |
Zebra fish, Cow, Human, Mouse, Rat |
- |
AKT1, AKT2 and AKT3 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide) |
ELISA, WB |
Zebra fish, Cow, Human, Mouse, Rat |
immunigen = synthetic peptide surrounding aa70 of the human Akt2 |
AKT1, AKT2 and AKT3 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
IH, WB |
Human |
immunogen = synthesized phosphopeptide derived from human Akt around the phosphorylation site of serine 473 (Q-F-Sp-Y-S). |
detects endogenous levels of Akt1 only when phosphorylated at Ser473 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, glycerol, affinity purified) |
IH, WB |
Human |
immunogen = synthesized non-phosphopeptide derived from human Akt around the phosphorylation site of serine 473 (Q-F-Sp-Y-S) |
endogenous levels of total Akt1 protein |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, glycerol, protein G purified) |
WB |
Human, Mouse, Rat |
immunogen = synthetic peptide corresponding to C-terminal residues of human Akt, (KLH coupled) |
Akt1 |
- |
|
|
Autogen Bioclear |
polyclonal
|
Rabbit
(liquid, serum, PBS, azide, glycerol, unpurified) |
ELISA, IF, IH, IP, WB |
Chicken, Human, Mouse, Rat |
immunogen = synthetic peptide, C R P H F P Q F S Y S A S G T A, corresp. to the C-terminus (460-480) of human, rat and mouse and chicken AKT proteins (KLH) |
Akt1 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
IH, WB |
Human |
immunogen = synthesized phosphopeptide derived from human Akt around the phosphorylation site of threonine 308 (M-K-Tp-F-C). |
detects endogenous levels of Akt1 only when phosphorylated at Threonine 308 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(lyophilized, PBS, sucrose, protein A/G purified) |
WB |
Dog, Human, Mouse |
immunogen = synthetic peptide corresp. to a region of human AKT1 |
Akt1 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG1 |
Mouse
(liquid, PBS, glycerol, no preservative, protein G purified) |
ELISA, FC, IC, IF, IP |
Human, Hamster, Mouse, Rat |
immunogen = recombinant protein corresp. to aa140-480 of human Akt1 |
endogenous levels of Akt1; no crossreaction with other related proteins |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Mouse
(PBS, azide, gelatin) |
IH, WB |
Human |
Immunogen = synthesized phosphopeptide derived from human Akt2 around the phosphorylation site of serine 474 (Q-F-Sp-Y-S) |
detects endogenous levels of Akt2 only when phosphorylated at serine 474 |
- |
|
|
Autogen Bioclear |
polyclonal
|
Rabbit
(liquid, KPBS, azide) |
ELISA, IF, IH, IP, WB |
Chicken, Human, Mouse, Rat |
immunogen = synthetic peptide, C R P H F P Q F S Y S A S G T A, corresp. to the C-terminus (460-480) of human, rat, mouse and chicken AKT proteins (KLH) |
Akt1/2/3 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(lyophilized, PBS, sucrose, affinity purified) |
ELISA, WB |
Dog, Human, Mouse |
immunogen = synthetic peptide corresp. to a region of human AKT1 |
Akt1 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Goat
(liquid, KPBS, azide, affinity purified) |
ELISA, IH, IP, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide corresp. to aa 455-468 (of 481) of human AKT2 conj. to KLH |
AKT1, AKT2 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IH, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide from N-terminus of human Akt1 |
AKT1, AKT2 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG |
Rabbit
(liquid, Tris-glycine saline, azide, glycerol, BSA) |
IF, IH, IP |
Bovine, Human, Mouse, Rat |
Immunogen = synthetic peptide corresp. to residues surrounding Ser473 of human Akt1 |
detects Akt1 and Akt3 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG |
Rabbit
(liquid, tris-glycine saline, azide, glycerol) |
IF, IH, WB |
Human, Mouse |
Immunogen = phospho specific peptide corresp. to residues surrounding Ser473 of human Akt1 |
detects Akt1 when phosphorylated at Serine 473 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG |
Rabbit
(liquid, Tris-glycine saline, azide, glycerol, BSA) |
FC, IC, IH, IP, WB |
Bovine, Human, Mouse, Rat |
Immunogen = synthetic peptide corresp. to residues in C-terminus of human Akt1 |
Akt1 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, glycerol, affinity purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide |
Akt1 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(lyophilized, PBS, sucrose, affinity purified) |
IH |
Dog, Human, Rat |
immunogen = synthetic peptide corresp. to a region of human AKT1 |
Akt1 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, HEPES, BSA, NaCl, azide, glycerol, purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to a region of human AKT1 |
Akt1 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, HEPES, BSA, NaCl, azide, glycerol, purified) |
WB |
Human, Mouse, Rat |
immunogen = synthetic peptide |
Akt1 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, glycerol, affinity purified) |
IH, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide corresp. to residues surrounding human Ser473 of Akt1 |
detects Akt1 when phosphorylated at Serine 473 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, glycerol, affinity purified) |
IH, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide corresp. to residues surrounding human Ser473 of Akt1 |
detects Akt1 when phosphorylated at Threonine 308 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IH |
Human, Mouse, Rat |
Immunogen = synthetic peptide corresp. to the C-terminus of AKT1/PKB alpha of human origin at the phosphorylated site of Serine 473 |
detects Akt1 when phosphorylated at Serine 473 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IH |
Human, Mouse, Rat |
Immunogen = synthetic peptide corresp. to the C-terminus of AKT1/PKB alpha of human origin at the phosphorylated site of Serine 473 |
detects Akt1 when phosphorylated at Serine 473 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Goat
(liquid, HEPES, NaCl, BSA, glycerol, azide, purified) |
WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide |
AKT2 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Goat
(liquid, PBS, glycerol, azide, affinity purified) |
IH, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide |
AKT2 phosphorylated at Ser474 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Goat
(liquid, TBS, BSA, azide, affinity purified) |
ELISA, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide internal region of AKT3: peptide sequence (C S P T S Q I D N I G E E E M) |
Akt3 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, glycerol, thimerosal, affinity purified) |
IH, IP, WB |
Cow, Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to residue surrounding aa80 of human Akt |
detects Akt1, Akt2 and Akt3 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, BSA, azide, protein A/G purified) |
IP, WB |
Human |
immunogen = recombinant full length human PRAS40 expressed in E. coli |
AKT1S1(40kD) |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide, affinity purified) |
IH, IP, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide mapping to the C-terminal domain of human Akt1 |
Akt1 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG2b |
Mouse
(liquid, ascites) |
ELISA, WB |
Human |
immunogen = Ni-NTA purified truncated recombinant Akt2 expressed in E. coli strain BL21 (DE3) |
Akt2 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, purified) |
WB |
Human |
Immunogen = synthetic peptide of human Akt2 |
AKT2 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, glycerol, affinity purified) |
FC, IP, WB |
Mouse |
immunogen = synthetic peptide mapping to the C-terminal domain of human Akt1 |
detects endogenous levels of total Akt1, Akt2 and Akt3 proteins; no cross-reaction with other related kinases |
- |
|
|
Autogen Bioclear |
monoclonal
IgG3 |
Mouse
(liquid, HEPES, NaCl, glycerol, affinity purified) |
WB |
Human, Mouse, Rat |
- |
Akt1 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
IP, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to residues of human Akt3 |
endogenous levels of Akt3; no crossreaction with recombinant Akt1 or Akt2 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, glycerol, affinity purified) |
WB |
Human, Mouse |
Immunogen = synthetic phosphopeptide derived from the sequence of human Akt 1 pSer473 |
Akt pSer473 |
- |
|
|
Autogen Bioclear |
polyclonal
|
Rabbit
|
WB |
Human, Mouse |
- |
Akt1 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
IF, WB |
Human, Mouse |
Immunogen = synthetic phosphopeptide derived from the region of human Akt that contains serine 473 (P Q F pS Y S A) |
Akt pSer473 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG |
Mouse
(liquid, PBS, azide, gelatin, protein G purified) |
IP, WB |
Human, Mouse |
immunogen = synthetic peptide containing phosphorylated serines at aa residues 473 of human Akt1 |
Akt1 phosphorylated at Ser473 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG2b |
Mouse
(liquid, HEPES, NaCl, BSA, glycerol, azide, protein G purified) |
ELISA, IH, IP, WB |
Human, Mouse, Rat |
immunogen = synthetic phosphopeptide corresp. to residues around Ser473 of mouse Akt (KLH coupled) |
Akt1 phosphorylated at Ser473; no detection of Akt phosphorylated at other sites or related kinases such as PKC and p70 S6 kinase |
- |
|
|
Autogen Bioclear |
monoclonal
IgG2a |
Mouse
(liquid, lysis buffer, glycerol, azide, purified) |
ELISA, IP, WB |
Human, Mouse, Rat |
immunogen = synthetic phosphopeptide corresp. to residues around Ser473 of human Akt (KLH coupled) |
Akt1 phosphorylated at Ser473; will also bind some nonphospho-Akt |
- |
|
|
Autogen Bioclear |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, affinity purified) |
IP, WB |
Chicken, Frog, Human, Mouse |
immunogen = synthetic peptide containing phosphorylated serines at aa residues 473 of human Akt1 |
Akt1 phosphorylated at Ser473 |
- |
|
|
Autogen Bioclear |
polyclonal
|
Rabbit
(liquid, PBS, azide) |
IF, WB |
Human, Mouse |
immunogen = synthetic phosphopeptide derived from the region of human Akt that contains serine 473 (P Q F pS Y S A) |
Akt1 phos Ser473 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified) |
ELISA, IH, WB |
Human, Mouse |
immunogen = synthetic phosphopeptide derived from the region of human Akt that contains serine 473 |
Akt1, 2, and 3 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG |
Rabbit
(liquid, Tris-glycine saline, glycerol, azide, BSA) |
WB |
Human, Mouse |
immunogen = synthetic phosphopeptide corresp. to residues surrounding Threonine 308 of human Akt1 |
Akt1 phosphorylated at Thr308 |
- |
|
|
Autogen Bioclear |
polyclonal
|
Rabbit
(liquid, PBS, azide) |
WB |
Human, Mouse |
immunogen = synthetic phosphopeptide corresp. to human Akt1 at the phosphorylation site of threonine 308 (T M K pT F C G) |
Akt1 phosphorylated at Thr308 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, thimerosal, glycerol, affinity purified) |
IP, WB |
Human, Mouse, Rat |
Immunogen = synthetic phosphopeptide derived from the sequence of human Akt 1 pSer473 |
detects Akt1 when phosphorylated at Ser473 and Akt2 and Akt3 when phosphorylated at equivalent sites |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, neat serum, unpurified) |
ELISA, WB |
Human, Mouse, Rat |
immunogen = 11 aa synthetic peptide seq. near the N-terminus regulatory domain of rat Akt3, KLH coupled |
Akt3; no crossreaction with Akt1, Akt2 or other kinases |
- |
|
|
Autogen Bioclear |
monoclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
FC, IF, IH, IP, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide (KLH-coupled) at the C-terminal sequence of mouse Akt |
endogenous levels of total Akt protein |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, unpurified serum, PBS, azide, glycerol) |
ELISA, WB |
Rat |
immunogen = synthetic peptide corresp. to ~C-terminal 16aa peptide of rat AKT1 |
Akt1 |
- |
|
|
Autogen Bioclear |
polyclonal
|
Rabbit
(liquid, unpurified serum, PBS, glycerol, azide) |
ELISA, WB |
Rat |
immunogen = synthetic peptide corresp. to rat AKT1 (~C-terminus, 15 aa) containing a phosphorylated Ser472, coupled to KLH |
Akt1 phos Ser472 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, sucrose, protein G purified) |
WB |
Dog, Human, Mouse |
Immunogen = synthetic peptide corresp. to a region of human AKT1 |
Akt1 |
- |
|
|
Autogen Bioclear |
polyclonal
|
Rabbit
(liquid, PBS, azide, glycerol, affinity purified) |
WB |
Human, Mouse |
immunogen = recombinant protein derived from E. coli-expressed mouse Akt alpha (aa 375-479) |
Akt1 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
WB |
Chicken, Cow, Human, Mouse, Rat |
immunogen = synthesized phosphopeptide derived from human Akt around the phosphorylation site of threonine 308 (T M-K-Tp-F-C G) |
Akt1 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
FC, IF, WB |
Human, Mouse, Rat |
immunogen = synthetic phosphopeptide corresp. to residues around Thr308 of mouse Akt (KLH-coupled) |
detects endogenous levels of Akt only when phosphorylated at Thr308 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
IF, WB |
Drosophila |
immunogen = synthetic phospho peptide corresp. to aa residues around Ser505 of drosophila Akt, KLH coupled |
detects endogenous levels of Akt1 when phos at Ser505; not Akt when phosphorylated at other sites; not related kinases such as PKC or p70 S |
- |
|
|
Autogen Bioclear |
monoclonal
|
Rabbit
(liquid, supernatant, HEPES, NaCl, BSA, glycerol, azide) |
ELISA, IF, IH |
Human, Mouse, Rat |
immunogen = synthetic phosphopeptide corresp. to residues around Thr308 of mouse Akt (KLH-coupled) |
detects endogenous Akt1 only when phosphorylated at Ser473 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG1 |
Mouse
(liquid, PBS, glycerol, protein G purified, conj. biotinylated) |
IP |
Human, Hamster, Mouse, Rat |
immunogen = recombinant protein corresp. to aa140-480 of human Akt1 |
detects endogenous levels of Akt. Does not cross-react with other related proteins |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, HEPES, BSA, azide, NaCl, glycerol, affinity purified) |
ELISA, FC, IF, IP, WB |
Chicken, Human, Hamster, Mouse, Rat |
immunogen = synthetic phosphopeptide corresp. to residues around Ser473 of mouse Akt (KLH coupled). |
detects endogenous levels of Akt1 only when phosphorylated at Ser473. Recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues. Does not recognize Akt phosphorylated at other sites. |
- |
|
|
Autogen Bioclear |
monoclonal
IgG1, κ |
Mouse
(lyophilized, purified) |
ELISA, WB |
Dog, Human, Mouse, Rat |
immunogen = synthetic peptide seq. (aa466-480) located at the C-terminus of Akt (KLH coupled) |
Akt1 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG1, κ |
Mouse
(lyophilized, purified) |
ELISA, WB |
Human, Mouse |
immunogen = synthetic peptide corresp. to C-terminal, Q F S Y S A, human Akt (KLH) |
recognizes Akt dephosphorylated at Ser473. Does not cross-react with Akt phosphorylated at Ser473 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified) |
ELISA, WB |
Human, Mouse, Rat |
immunogen = 16-aa peptide near the C-terminus of rat AKT2 (rat/mouse/human 481 aa) with an added N-terminal cysteine, conjugated to KLH |
Akt2; no cross reaction with Akt1 or Akt3 or other kinases |
- |
|
|
Autogen Bioclear |
monoclonal
IgG |
Mouse
(liquid, PBS, BSA, azide, protein A/G purified) |
WB |
Human |
immunogen = synthetic peptide derived from the sequence of human PRAS40 (KLH-coupled) |
detects endogenous levels of total PRAS40 protein |
- |
|
|
Autogen Bioclear |
polyclonal
|
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, no preservative, affinity purified) |
FC, IH, IP, WB |
Human, Hamster, Mouse, Rat |
immunogen = synthetic phosphopeptide corresp. to residues around Thr 308 of mouse AKT1 (KLH) |
Akt1 phosphorylated at Thr308. Does not detect Akt phosphorylated at other sites or related kinases such as PKC and p70 S6 kinase |
- |
|
|
Autogen Bioclear |
monoclonal
IgG1, κ |
Mouse
(lyophilized, purified) |
ELISA, WB |
Dog, Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to a portion of human Akt phosphorylated at Ser473 |
Akt1 phosphorylated at Ser473 |
- |
|
|
Autogen Bioclear |
polyclonal
|
Rabbit
(liquid, PBS, azide, affinity purified) |
ELISA, WB |
Chicken, Human, Mouse, Rat |
immunogen = synthetic peptide derived from the C-terminal region of the mouse Akt (protein kinase B) protein, phosphorylated at Ser473 |
Akt1 phos Ser473 |
- |
|
|
Autogen Bioclear |
polyclonal
IgY |
Chicken
(liquid, PBS, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide: N S L K Q R A P G E D P M D Y K C, corresp. to aa108-124 of human AKT2 |
Akt2 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG2b |
Mouse
(liquid, BBS, no preservative, purified) |
ELISA, WB |
Human, Mouse |
- |
Akt1 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG2a |
Mouse
(liquid, BBS, no preservative, purified) |
ELISA, IP, WB |
Human |
- |
Akt |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, glycerol, affinity purified) |
ELISA, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to ~C-terminal 16aa peptide of rat AKT1 |
Akt1; may have some reactivity with the phosphorylated form of AKT1 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, BSA, protein G purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide corresponding to aa 119-136 of human Akt3 (protein kinase B; PKB ) |
Akt3 (~50kD) |
- |
|
|
Autogen Bioclear |
monoclonal
IgG2b |
Mouse
(liquid, ascites) |
ELISA, WB |
Human |
immunogen = Ni-NTA purified truncated recombinant Akt2 expressed in E. coli strain BL21 (DE3) |
Akt2 |
- |
|
|
Autogen Bioclear |
monoclonal
IgG2a |
Mouse
|
ELISA, WB |
Human |
immunogen = synthetic peptide corresp. to a portion of Akt2 |
Akt2 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, BSA, affinity purified) |
WB |
Human, Mouse |
immunogen = synthetic phosphopeptide derived from the region of human PRAS40 that contains threonine 246 |
Akt1 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, glycerol, affinity purified) |
WB |
Human, Mouse |
Immunogen = synthetic phosphopeptide derived from the C-terminus region of human Akt1 |
Akt1; cross with human Akt2 and Akt3 |
- |