|
|
B24.2(Bart De Strooper,K.U.Leuven, Belgium)PaperReference: Zhao, 2010 |
polyclonal
|
Rabbit
(liquid, supernatant) |
WB |
Mouse |
immunogen = the peptide KLDPSSQGAL QLPYDPEMEE (amino acids 306–325), coupled to KLH |
detects C-terminal fragments of PS-2 |
- |
|
|
anti-PS1-NT1(Paul Mathews, New York University, School of Medicine,Center for Dementia Research)produced by CovanceReference: Gu, 2002 |
monoclonal
IgG1 |
Mouse
(purified) |
IP, WB |
Primate (various), Human |
directed against residues 41-49 (RRSLGHPEP) of PS-1 |
recognizes full length PS-1 and the ~28 kDa amino-terminal fragment produced by in vivo proteolysis in L cells overexpressing PS1, but not proteins in mouse L cells overexpressing human PS2. NT1 recognized multiple bands migrating between 28 kDa and 35 kDa from human prefrontal cortex |
- |
|
|
anti-PS1-C(Hui Zheng, Huffington Center on Aging, Baylor College of Medicine)used in paper by Xia, 2002 |
polyclonal
|
Rabbit
|
WB |
- |
aa 454-467 of PS1 with glutamate residue added to N terminus to enhance solubility |
|
- |
|
|
B17(Bart De Strooper,K.U.Leuven, Belgium)Reference: Sato, 2007 |
polyclonal
|
Rabbit
|
IP |
Human, Mouse |
immunogen = peptide p46 (EGDPEAQR RVSKNSKC), situated in the hydrophilic loop domain of PS1Paper |
PS1, hydrophilic loop-specific |
- |
|
|
PS1-BI.3D7(Christian Haass,Adolf Butenandt Institute, University of Munich)used in paper by Steiner, 2002 |
monoclonal
|
|
- |
- |
fusion protein, C-terminal (aa 263-407) |
N terminus of PS-2 |
- |
|
|
PS2 (GL2)(Takeshi Iwatsubo, Department of Neuropathology & Neuroscience, Grad. School of Pharmaceutical Sciences, University of Tokyo)References:Zhao, 2010Yu, 2001 |
polyclonal
|
Rabbit
|
IH, WB |
Human |
immunogen = GST fused to amino acids 301–361 of human PS2 |
detects C-terminus of PS-2 |
- |
|
|
anti-αPS1 Loop(Gopal Thinakaran, Department of Neurobiology, University of Chicago)References:Veeraraghavalu, 2010Yu, 2001Luo, 2003 |
polyclonal
|
Rabbit
|
IH |
Human, Mouse |
immunogen = aa 320-375 of human PS1 |
reacts with epitopes in the hydrophilic loop domain (amino acids 263–407) of human and mouse PS1; no cross reactivity of PS1Loop antiserum with the human PS2 fusion protein |
- |
|
|
PS1NT(Gopal Thinakaran, Department of Neurobiology, University of Chicago)References: Veeraraghavalu, 2010O'Connor, 2008Ikeuchi, 2003 |
polyclonal
|
Rabbit
|
WB |
- |
(epitope contains residues 1–65 of the N-terminus of PS1) |
full-length PS1 and NH2-terminal fragment of PS1 |
- |
|
|
4627Dr. Dennis Selkoe,Harvard Univ. Medical School, Boston, MAReference: Sato, 2007 |
polyclonal
|
Rabbit
|
IP |
Human, Hamster, Mouse |
immunogen = synthetic peptide corresp. to the C-terminal 10 amino acids of PS1 (aa 457-467) Paper |
extreme C-terminal PS1, and PS1 CTF |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, ascites, no preservative) |
IC, IH, IP, WB |
Primate (various), Mouse, Rat |
immunogen = Presenilin-1, loop fusion protein, C-terminal (amino acids 263-378), human |
Presenilin-1, loop. |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, PBS, azide) |
IC, IF, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide (Human) of 13 amino acids from near the C-terminus of PEN2 |
detects Carboxy terminal end of PEN2 protein |
- |
|
|
Zymed/ Invitrogen |
polyclonal
|
Rabbit
(liquid, PBS, azide, affinity purified) |
IP, WB |
Human, Mouse |
immunogen = synthetic peptide derived from the C-terminal region of human Aph-1a |
Aph-1a (~23kDa) long isoform only |
- |
|
|
A B R - Thermo Scientific |
polyclonal
IgG |
Rabbit
(epitope affinity purified IgG, PBS, BSA, azide) |
WB |
Human, Mouse |
immunogen = synthetic peptide corr. to aa 688-708 of human nicastrin; one aa substitution between human and mouse sequence |
Nicastrin in tissues and cells; ~110 kDa in WB |
- |
|
|
A B R - Thermo Scientific |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide) |
WB |
Human, Mouse, Rat |
immunogen = a 18 amino acid peptide sequence near the internal region of human nicastrin |
detects Nicastrin |
- |
|
|
A B R - Thermo Scientific |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide) |
WB |
Human, Mouse, Rat |
immunogen = a 17 amino acid peptide sequence near the carboxy terminus of human nicastrin |
detects Nicastrin |
- |
|
|
A B R - Thermo Scientific |
polyclonal
|
Rabbit
(liquid, serum, azide) |
IP, WB |
Human |
immunogen = synthetic peptide corresp. to aa residues F Q I Y R P R W G A L G D Y L S F T I P L G T P of human Pen2 |
Pen-2 |
- |
|
|
A B R - Thermo Scientific |
polyclonal
|
Rabbit
(liquid, PBS, azide) |
WB |
Human, Mouse, Rat |
immunogen = a 13 amino acid peptide sequence near the amino terminus of human PEN2. |
detects Pen-2, N-terminal |
- |
|
|
A B R - Thermo Scientific |
polyclonal
IgG |
Rabbit
(IgG affinity purified, lyophilized) |
WB |
Human, Hamster, Mouse |
immunogen = a synthetic peptide, conjugated to KLH, corresponding to the 11 most N-terminal residues of human Pen-2 |
detects Pen-2 |
- |
|
|
A B R - Thermo Scientific |
polyclonal
|
Rabbit
(liquid, affinity purified, PBS, BSA, azide, glycerol) |
IF, IP, WB |
Human, Mouse |
immunogen = Synthetic peptide, KLH conjugated, corresponding to residues C(245)RRQEDSRVM VYSALRIPPED(265) of human APH-1aL/C-terminal |
recognizes APH-1 |
- |
|
|
Sigma Aldrich |
polyclonal
|
Rabbit
(buffered aqueous solution , IgG fract, PBS, azide) |
WB |
Human |
immunogen = synthetic peptide corr to C-terminus, aa 693-709 of human nicastrin, conj. to KLH |
detects Nicastrin |
- |
|
|
Santa Cruz |
monoclonal
IgG2b |
Mouse
(liquid, PBS, azide, protein stabilizer) |
IF, IP, WB |
Human |
immunogen = amino acids 1-80 of Presenilin 1 of human origin |
for detection of Presenilin 1 NTF subunit of human origin; no cross with Presenilin 2 or Presenilin 1 CTF subunit |
- |
|
|
Calbiochem EMD Biosciences |
polyclonal
IgG |
Rabbit
(affinity purified IgG, PBS, azide, gelatin) |
IH, WB |
Human, Mouse, Rat |
synthetic peptide (EEDSYDSFGEPS) corr. to aa 324-335 from the C-terminus of human PS2 |
detects ~20 kDa proteolytic fragment of presenilin-2 |
- |
|
|
Calbiochem EMD Biosciences |
polyclonal
IgG |
Rabbit
(liquid, purified, PBS, azide, gelatin or glycerol) |
IB, IF |
Mouse, Rat |
immunogen = aa 31-46 of N-terminus of mouse PS-1 |
recognizes the ~98 kDa presenilin-1 dimer. |
- |
|
|
Calbiochem EMD Biosciences |
polyclonal
IgG |
Rabbit
(liquid, purified, PBS, azide, gelatin) |
IF, WB |
Mouse, Rat |
immunogen = synthetic peptide corrsp. to aa 303-316 of the intracellular loop region of mouse PS-1 |
~98k Presenilin-1 dimer |
- |
|
|
Calbiochem EMD Biosciences |
polyclonal
IgG |
Goat
(liquid, undiluted serum, azide) |
ELISA, IH |
Human |
immunogen = synthetic peptide corresp. to aa 14-33 of human presenilin-1 |
presenilin-1 protein |
- |
|
|
Calbiochem EMD Biosciences |
polyclonal
IgG |
Rabbit
(liquid, undiluted serum, no preservative) |
IB, IF, IP |
Human, Monkey, Mouse, Rat |
immunogen = recomb. protein containing the loop domain (aa 263-407) of human presenilin 1 fusedto GST |
Recognizes full-lengthpresenilin 1 and its C-terminal fragments |
- |
|
|
A D I |
polyclonal
IgG |
Rabbit
(affinity purified with PBS, BSA and merthiolate) |
ELISA, IF, IH, IP, WB |
Human, Mouse, Rat, Xenopus |
immunogen = a 15 mer peptide (PS11-P) locating to the large hydrophilic loop of human or mouse Presenilin-1 |
Presenilin-1 |
- |
|
|
A D I |
polyclonal
|
Rabbit
(unpurified antiserum) |
ELISA, IF, IH, IP, WB |
Human, Mouse, Rat, Xenopus |
immunogen = a 15 mer peptide (PS11-P) locating to the large hydrophilic loop of human or mouse Presenilin-1 |
Presenilin-1 |
- |
|
|
A D I |
polyclonal
|
Goat
(unpurified antiserum) |
ELISA, IF, IH, IP, WB |
Human |
immunogen = a 20-aa peptide from human Presenilin-1, at the N-terminus, extracellular region |
Presenilin-1; no cross with PS2 or other known proteins |
- |
|
|
A D I |
polyclonal
IgG |
Rabbit
(affinity purified with PBS, BSA and merthiolate) |
ELISA, IF, IH, IP, WB |
Human, Mouse, Rat |
immunogen = a 18 mer peptide (PS21-P) locating near the amino terminus of Presenilin-2 |
Presenilin-2 |
- |
|
|
A D I |
polyclonal
|
Rabbit
(unpurified antiserum) |
ELISA, IF, IH, IP, WB |
Human, Mouse, Rat |
immunogen = a 18 mer peptide (PS21-P) locating near the amino terminus of Presenilin-2 |
Presenilin-2 |
- |
|
|
BioVision |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, PBS, glycerol, BSA, azide) |
WB |
Human, Mouse, Rat |
immunogen = synthetic peptide surrounding amino acid 296 of human Presenilin 1 |
Presenilin-1 |
- |
|
|
BioVision |
polyclonal
|
Rabbit
(liquid, PBS, glycerol, BSA, thimerosal, affinity purified) |
IH, IP, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide surrounding amino acid 333 of human presenilin 2 |
Presenilin-2 |
- |
|
|
Vinci Biochem |
polyclonal
IgG |
Rabbit
(epitope affinity purified IgG, PBS, BSA, azide) |
WB |
Human, Mouse |
immunogen = synthetic peptide corr. to aa 688-708 of human nicastrin; one aa substitution between human and mouse sequence |
Nicastrin in tissues and cells; ~110 kDa in WB |
- |
|
|
VWR International, Inc. |
monoclonal
IgG1 |
Mouse
(liquid Ig, purified, PBS, azide) |
IC, IH, IP, WB |
Primate (various), Human, Mouse, Rat |
aa 263-378 of Presenilin-1, loop fusion protein |
Presenilin-1, loop. |
- |
|
|
VWR International, Inc. |
polyclonal
IgG |
Rabbit
(affinity purified IgG, PBS, azide, gelatin) |
IH, WB |
Human, Mouse, Rat |
synthetic peptide corr. to aa 324-335 from the C-terminus of human PS2 |
~20 kDa proteolytic fragment of presenilin-2 |
- |
|
|
VWR International, Inc. |
monoclonal
IgG2a |
Rat
(liquid, supernatant, no preservative) |
ELISA, IH, IP, WB |
Human |
N-terminal of human PS-1 aa 21-80 fused to GST |
neuritic component of senile plaques as well as in neurofibrillary tangles |
- |
|
|
VWR International, Inc. |
polyclonal
|
Rabbit
(serum, liquid, PBS, azide) |
IC, IP, WB |
Human, Monkey, Hamster, Mouse |
aa 275-367 of human PS1 fused to C-terminus of maltose binding protein |
Presenilin 1, loop |
- |
|
|
BD Transduction |
monoclonal
IgG2a |
Mouse
(liquid, PBS, BSA, glycerol, azide) |
WB |
Dog, Chicken, Human, Mouse, Rat |
immunogen = peptide corresp. to aa 168-289 from human nicastrin |
nicastrin (~110 kDa) |
- |
|
|
Calbiochem EMD Biosciences |
polyclonal
IgG |
Rabbit
(liquid, undiluted serum, no preservative) |
IF, IP, WB |
Human, Monkey, Mouse, Rat |
immunogen = recomb. protein containing aa 1-65 of human presenilin 1 fused to GST |
Recognizes the presenilin 1 (1-65) holoprotein and its N-terminal fragments |
- |
|
|
None |
polyclonal
|
Rabbit
(serum, concentrated, no preservative) |
IP, WB |
Human |
immunogen = peptide (T E L P A P L S Y F N R K C) conj. to KLH |
presenilin 1 |
Discontinued |
|
|
None |
polyclonal
IgG |
Rabbit
(liquid, purified, PBS, no carrier, no preservative) |
IP, WB |
Human |
immunogen = aa 698-709 of nicastrin |
nicastrin protein |
Discontinued |
|
|
None |
monoclonal
IgG1 |
Mouse
(liquid, supernatant) |
IF, WB |
Human |
immunogen = GST-fusion containing the complete N-terminal fragment of human PS2 (residues 1-87) |
presenilin 2 |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(liquid, affinity purified) |
IH, WB |
Human |
immunogen = human Aph-1aL/S Loop region 92-115 with seq C K L L K K A D E G L A S L S E D G R S P I S I R |
Aph-1aL/S loop |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(liquid, affinity purified) |
IH, WB |
Human |
immunogen = human Aph-1b/C-terminal region sequence C Q D K N F L L Y N Q R S R Q D K N F L L Y N Q R S R of human Aph-1b. |
Aph-1b; some reactivity with Aph-1aL/S |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(liquid, affinity purified) |
IF, IP, WB |
Human, Mouse |
immunogen = C-terminal fragment of nicastrin corresp. to a peptide containing residues 691-709 [C]-N A K A D V L F I A P R E P G A V S Y, conj. to KLH |
nicastrin |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(liquid, affinity purified) |
IF, IP, WB |
Human, Mouse |
immunogen = N-terminal fragment of nicastrin corresp. to a peptide containing residues 103-124 (C F T R D L M E K L K G R T S R I A G L A V S L), coupled to KLH |
nicastrin |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(liquid, serum) |
IP, WB |
Human |
immunogen = N-terminal residues of Pen-2 and recognizes the residues E R V S N E E K L N L of Pen-2 |
Pen2 (12kDa) |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(liquid, serum) |
IP, WB |
Human |
immunogen = C-terminal residues of Pen-2 and recognizes the residues F Q I Y R P R W G A L G D Y L S F T I P L G T P of Pen-2 |
Pen2 |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(affinity purified) |
IF, IP, WB |
Human |
immunogen = cytoplasmic loop (or C-terminal fragment of PS1) corresp. to a peptide containing residues 368-390[C I L A G E D P E E R G V K L G L G D F I F Y S] |
Presenilin 1 |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(affinity purified) |
IF, IP, WB |
Human |
immunogen = peptide 1-25 of the human PS1 C M T E L P A P L S Y F Q N A Q M S E D N H L S, coupled to KLH |
presenilin 1 |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(liquid, BSA, glycerol, azide, affinity purified) |
IH, WB |
Human |
immunogen = human Aph-1b/loop region and recognizes the sequence C K L L K K A S E G L K S I N P G E T A P S M R |
Aph-1b/loop region |
Discontinued |
|
|
BioLegend |
polyclonal
|
Rabbit
(liquid, affinity purified) |
IH, WB |
Human |
immunogen = human Aph-1aL/ C-terminal region sequence C R R Q E D S R V M V Y S A L R I P P E D |
Aph-1aL |
Available from BioLegend |