|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin) |
FC, IF, IP, WB |
Human, Mouse, Rat |
immunogen = amino acids610-723 mapping at the C-terminus of human HSP 90β |
detection of HSP 90α and HSP 90β |
- |
|
|
BD Transduction |
monoclonal
IgG1 |
Mouse
(aqueous, buffered, BSA, azide, glycerol) |
WB |
Dog, Chicken, Human, Mouse, Rat |
immunogen = human Hsp75/TRAP1 (epitope is withinaa residues 571-685) |
detects ~75 kDa TRAP1 |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(protein G purified) |
ELISA, FC, IC, IF, IH, IP, WB |
Bovine, Dog, Rabbit, Chicken, Carp, Sheep, Pig, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat, C. elegans, Drosophila |
immunogen = recombinant hsp70/hsc70 mapping in the region of aa 436-503 |
hsp70; no cross-reactivity to hsc70 (hsp73) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(protein A purified) |
FC, IF, IH, IP, WB |
Human |
- |
hsp70 |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(protein G purified) |
FC, IF, IH, IP, WB |
Plant (various), Bovine, Dog, Porcine, Rabbit, Whale, Chicken, Carp, Rainbow trout, Sheep, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat, Xenopus, C. elegans, Drosophila |
immunogen = recombinant hsp70/hsc70 |
recognizes 72 and 73kD proteins corresp. to the molecular mass of inducible hsp and hsc70 |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(protein A purified) |
FC, IF, IH, IP, WB |
Bovine, Human, Monkey |
immunogen = HSP70 protein from HeLa cells |
recognizes both the constitutive (HSP73) and inducible (HSP72) forms of HSP70 |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(ascites) |
IC, IH, IP, WB |
Avian (various), Amphibian (various), Fish (various), Yeast, Human, Mouse, Drosophila |
immunogen = recombinant human HSP70 (heat shock protein 70) |
recognizes several members of the HSP70 gene family including HSP70, HSC70, p75, and HSP72; by WB detects bands at ~70kD to ~78kDa |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
|
FC, IH, IP, WB |
Human |
immunogen = HSP70 from HeLa cells |
reacts with both the constitutive (HSP73) and inducible (HSP72) forms of HSP70 |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(unpurified serum) |
IH, IP, WB |
Human |
immunogen = purified heat shock protein (DnaK) from E.coli |
reacts with both the constitutive (HSP73) and inducible (HSP72) forms of HSP70 |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(affinity purified) |
ELISA, IH, WB |
Human, Monkey, Mouse, Rat |
immunogen = synthetic peptide (KLH-coupled) corresp. to human HSP70 |
endogenous levels of total HSP70 protein (HSP70-Hom, HSP70-1); no crossreaction with other HSPs |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(ascites) |
IH, IP, WB |
Avian (various), Fish (various), Frog, Wheat, Yeast, Human, Mouse, Drosophila |
immunogen = human HSP70 |
recognizes HSP70, HSC70, and following heat shock, HSP72; detects ~70 to ~72kDa bands in WB |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(ascites) |
IC, IP, WB |
Avian (various), Fish (various), Frog, Wheat, Human, Mouse, Drosophila |
immunogen = recombinant HSP70 (heat shock protein 70) |
recognizes HSP70, HSC70, GRP78, and following heat shock, HSP72; detects ~70 to ~78kDa bands in WB |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(unpurified serum) |
IH, IP, WB |
Human, Mouse, Rat |
immunogen = purified heat shock protein (DnaK) from E.coli |
reacts with both the constitutive (HSP73) and inducible (HSP72) forms of HSP70 |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(DEAE-column chromatography) |
ELISA, IH, WB |
Human |
immunogen = full-length human hsp70 protein |
recognizes both the inducible form (hsp70) and the constitutive form (hsc70) of the hsp70 protein |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(protein G purified) |
ELISA, WB |
Human |
immunogen = recombinant HSP 70 M. tuberculosis |
Hsp70 |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(protein G purified) |
ELISA, WB |
Human |
immunogen = Heat Shock Protein 70 (HSP70) |
Hsp70 |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(protein G purified) |
ELISA, WB |
Human |
immunogen = Heat Shock Protein 70 (HSP70) |
Hsp70 |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(protein A purified) |
ELISA, WB |
Bovine, Cat, Dog, Goat, Human |
immunogen = Recombinant human HSP70 |
Hsp70 |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(ascites) |
IP, WB |
Human |
immunogen = recombinant human HSP70 |
Hsp70 |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(unpurified serum) |
IC, WB |
Human, Rat |
immunogen = purified heat shock protein (DnaK) from E.coli |
HSP 72 and HSP 73 HSP70 |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(unpurified serum) |
IP, WB |
Bovine, Dog, Porcine, Rabbit, Chicken, Rainbow trout, Sheep, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat, Drosophila |
immunogen = recombinant rat Hsp70 protein fragment (residues 446-641) |
HSP70/Hsc70 |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Rat
(ascites) |
WB |
Yeast, Human, Mouse, Trypanosome, Drosophila |
immunogen = denatured Drosophila HSP70 (heat shock protein 70) (aa 437-479) |
HSP70, HSC70, GRP78, and following heat shock, HSP72 |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(unpurified serum) |
WB |
Carp, Rainbow trout |
immunogen = recombinant chinook salmon Hsp70 protein |
detects ~70kD and ~73kD proteins, corresp. to the apparent molecular mass of Hsp70 (Hsp72) and Hsc70 (Hsp73) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(DEAE-column chromatography) |
WB |
Fish (various), Plant (various), Bovine, Dog, Porcine, Rabbit, Chicken, Sheep, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat, Xenopus, Mussel |
immunogen = purified Hsc70 and Hsp70 isolated from human HeLa cells |
detects 72 and 73kD proteins, corresp. to the apparent molecular masses of the inducible hsp70 and constitutive hsc70 |
- |
|
|
Novus Biologicals |
polyclonal
|
Sheep
(antisera, BSA, azide) |
WB |
Bovine, Human, Mouse |
immunogen = C-terminal fragment of the human HspBP1 protein |
specific for HspBP1 |
- |
|
|
AnaSpec |
monoclonal
IgG2a |
Mouse
(liquid, ascites,protein-A purified, PBS, BSA, azide) |
FC, IF, IH, IP, WB |
Cow, Human, Monkey |
immunogen = a HSP70 protein derived from HeLa cells |
detects Hsp70 |
- |
|
|
AnaSpec |
polyclonal
IgG2a |
Rabbit
(liquid, protein-A purified, PBS, BSA, azide) |
IF, IH, IP, WB |
Human, Mouse, Rat |
immunogen = a synthetic peptide corresponding to the N-terminus of amino acids 2-12 mouse heat shock protein 90α |
detects Hsp90α |
- |
|
|
AnaSpec |
polyclonal
IgG2a |
Rabbit
(liquid, affinity purified, PBS, BSA, azide) |
IH, WB |
Human, Mouse, Rat |
immunogen = a synthetic peptide corresponding to amino acids 31-50 of human HSP60 protein |
detects Hsp60 |
- |
|
|
AnaSpec |
polyclonal
IgG2a |
Rabbit
(liquid, affinity purified, PBS, BSA, azide) |
IH, WB |
Human, Mouse, Rat |
immunogen = a synthetic peptide corresponding to amino acids 31-50 of human HSP60 protein |
detects Hsp60 |
- |
|
|
AnaSpec |
polyclonal
IgG2a |
Rabbit
(liquid, protein-A purified, PBS, BSA, azide) |
IF, IH, IP, WB |
Human, Mouse, Rat |
immunogen = a synthetic peptide corresponding to the N-terminus of amino acids 2-13 mouse heat shock protein 90β |
detects hsp90β |
- |
|
|
Stressgen Enzo Life Sciences |
monoclonal
IgG2a |
Mouse
(liquid, PBS, azide, glycerol, protein-G purified) |
WB |
Rabbit, Human, Mouse, Rat |
immunogen = chicken Hsp70/Hsp90 complex |
detects HSP70 |
- |
|
|
Stressgen Enzo Life Sciences |
monoclonal
IgG1 |
Mouse
(liquid IgG, PBS, azide, glycerol, affinity purifiedAlso available as AP conj., ADI-SPA-820APBiotin conj., ADI-SPA-820B,DyLight™ 488 conjugate, ADI-SPA-810-488) |
ELISA, IH, WB |
Fish (various), Bovine, Dog, Rabbit, Chicken, Sheep, Pig, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat, Beluga, C. elegans, Drosophila |
epitope is inthe region of amino acid residues 436-503 of human Hsp70 |
detects ~70 kDa HSP70 |
- |
|
|
Stressgen Enzo Life Sciences |
monoclonal
IgG1 |
Mouse
(liquid PBS, glycerol, azide, protein G affinity purified) |
FC, WB |
Fish (various), Plant (various), Bovine, Dog, Rabbit, Chicken, Sheep, Pig, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat, Beluga, Xenopus |
Immunogen = purified Hsc70 and Hsp70 isolated from human HeLa cells |
detects inducable Hsp70 and constitutive Hsc70 |
- |
|
|
Stressgen Enzo Life Sciences |
monoclonal
IgG1 |
Mouse
(liquid, PBS, glycerol, azide, protein G affinity purified) |
WB |
Human |
Immunogen = native human Hsp70 protein |
detects human Hsp70 |
- |
|
|
Stressgen Enzo Life Sciences |
monoclonal
IgG1 |
Mouse
(liquid, PBS, glycerol, azide, protein G affinity purified) |
WB |
Human |
Immunogen = native human Hsp70 protein |
detects human and bacteria Hsp70 |
- |
|
|
Stressgen Enzo Life Sciences |
monoclonal
IgG1 |
Mouse
(liquid, PBS, glycerol, azide, protein G affinity purified) |
WB |
Human |
Immunogen = native human Hsp70 protein |
detects human Hsp70 |
- |
|
|
Stressgen Enzo Life Sciences |
monoclonal
IgG1 |
Mouse
(liquid, PBS, glycerol, azide, protein G affinity purified) |
WB |
Human |
Immunogen = native human Hsp70 protein |
detects human and bacteria Hsp70 |
- |
|
|
Stressgen Enzo Life Sciences |
polyclonal
|
Rabbit
(liquid, affinity purified) |
IP, WB |
Yeast, S. cerevisiae |
immunogen = 15 residue synthetic peptide, DDDNEDSM EIDDDLD, based on the S. cerevisiae Hsp104 (residues 894-908), conjugated to KLH |
recognizes Hsp104 |
- |
|
|
Stressgen Enzo Life Sciences |
polyclonal
|
Rabbit
(liquid, PBS, azide, glycerol, protein-A purified) |
WB |
Plant (various), Bovine, Dog, Rabbit, Chicken, Yeast, Sheep, Pig, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat |
immunogen = synthetic peptide derived from the sequence of rat Hsp70 |
recognizes HSP70 |
- |
|
|
Stressgen Enzo Life Sciences |
polyclonal
|
Rabbit
(liquid, PBS, azide, glycerol, affinity purified) |
IP, WB |
Bovine, Dog, Whale, Coral, Pig, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat, Beluga, C. elegans |
immunogen = recomb. human Hsp70 protein |
recognizes ~70 kDa HSP70 |
- |
|
|
Stressgen Enzo Life Sciences |
monoclonal
IgG1 |
Mouse
(liquid Tris, saline, MgCl2, glycerol, purified, AP conjugatedAlso available as R-PE conj., ADI-SPA-820PE, orDyLight™ 488 conjugate, ADI-SPA-820-488) |
WB |
Fish (various), Plant (various), Bovine, Dog, Rabbit, Chicken, Sheep, Pig, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat, Beluga, Xenopus, Mussel, C. elegans |
Immunogen = purified Hsc70 and Hsp70 isolated from human HeLa cells |
detects inducable Hsp70 and constitutive Hsc70 |
- |
|
|
Pierce Thermo Scientific |
unknown
|
Unknown Host
|
WB |
Human |
- |
specific for HSP70-1 |
- |
|
|
A B R - Thermo Scientific |
monoclonal
|
Mouse
(liquid, protein-G affinity purified, PBS, azide) |
ELISA, FC, IC, IH, IP, WB |
Bovine, Dog, Ovine, Porcine, Rabbit, Chicken, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat, C. elegans, Drosophila |
immunogen = human Hsp70 purified from HeLa cells, corresponding to amino acids 436-503 |
specific for HSP-70; does not cross-react with Hsc70 |
- |
|
|
A B R - Thermo Scientific |
monoclonal
IgM |
Mouse
(liquid, purified, PBS, azide) |
IF, IH, WB |
Avian (various), Amphibian (various), Fish (various), Yeast, Human, Mouse, Rat, Drosophila |
immunogen = human recombinant HSP70 over expressed in E. coli |
detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70 and, following heat shock, HSP72 |
- |
|
|
A B R - Thermo Scientific |
monoclonal
IgG1 |
Mouse
(liquid, purified, PBS, azide) |
EMSA, IF, IH, IP, WB |
Avian (various), Amphibian (various), Fish (various), Yeast, Human, Mouse, Rat, Drosophila |
immunogen = human recombinant HSP70 over expressed in E. coli |
detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70 and, following heat shock, HSP72 |
- |
|
|
A B R - Thermo Scientific |
monoclonal
IgG3 |
Mouse
(liquid, protein-G affinity purified, PBS, azide) |
IC, IP, WB |
Dog, Primate (various), Human, Mouse |
immunogen = Synthetic peptide corresponding to residues G(615) S G S S G T G E Q K E D Q K E E K Q(633) of mouse mtHSP70 |
detects mitochondrial heat shock protein 70 kDa (mtHSP70) |
- |
|
|
BD Transduction |
monoclonal
IgG1 |
Mouse
(aqueous, buffered solution, BSA, azide, glycerol) |
IF, WB |
Human, Mouse, Rat |
immunogen = human Hsp60 aa. 1-200 |
detects Hsp60 |
- |
|
|
Synaptic Systems |
monoclonal
IgG1 |
Mouse
(purified IgG, lyophilized) |
IC, WB |
Cow, Human, Hamster, Mouse, Rat |
immunogen = purified bovine ATPase (epitope between aa 391-546) |
detects Hsp70, specific for uncoating ATPase |
- |
|
|
Pierce Thermo Scientific |
monoclonal
IgG2a |
Rat
(liquid, Protein G purified, PBS) |
ELISA, FC, IC, IH, IP, WB |
Bovine, Dog, Ovine, Porcine, Rabbit, Chicken, Various species, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat |
immunogn = full length, native Hsc70 protein purified from sodium arsenite treated heat-resistant variants of Chinese hamster cells |
specific for Hsc70 |
- |
|
|
Stressgen Enzo Life Sciences |
monoclonal
IgG2a |
Rat
(liquid, PBS, azide, glycerol, affinity purified) |
ELISA, FC, IC, IF, IH, IP, WB |
Bovine, Dog, Chicken, Turtle, Sheep, Pig, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat |
immunogen = native hamster Hsc70 (Hsp73) |
recognizes ~73 kDa Hsc70 |
- |