|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, serum) |
ELISA, IH, WB |
Bovine, Rabbit, Chicken, Sheep, Human, Mouse, Rat |
immunogen = synthetic corresp. to C-terminal sequence of sheep 14-3-3ε |
14-3-3ε (C-terminal, 30kDa) |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, serum) |
ELISA, IH, WB |
Bovine, Rabbit, Chicken, Sheep, Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to acetylated N-terminal sequence of sheep 14-3-3ε |
14-3-3ε (N-terminal, 30kDa) |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Mouse
(liquid, PBS, unpurified serum) |
ELISA, WB |
Human |
immunogen = human YWHAE (aa1-256) with GST Tag |
14-3-3 protein epsilon (YWHAE) |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(lyophilized, affinity purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to a region of human 14-3-3η (YWHAH) |
14-3-3 protein eta(YWHAH) |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, serum) |
IH, WB |
Bovine, Rabbit, Chicken, Sheep, Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to acetylated N-terminal sequence of sheep 14-3-3 eta |
14-3-3 protein eta(YWHAH) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, glycerol, no preservative, affinity purified) |
IF, IH, IP, WB |
Human |
immunogen = recombinant human 14-3-3 sigma protein (26-30kD) |
14-3-3 protein σ; no cross with other isoforms of 14-3-3 |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, glycerol, no preservative, affinity purified) |
IF, IH, IP, WB |
Human |
immunogen = recombinant human 14-3-3 sigma protein (26-30kD) |
14-3-3 protein σ; no cross with other isoforms of 14-3-3 |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgY |
Chicken
(liquid, PBS, no preservative, affinity purified) |
IF, IH, IP, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to aa1-248 |
14-3-3 protein σ; (Stratifin) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, PBS, BSA, azide) |
IH, IP, WB |
Human, Rat |
immunogen = recombinant human 14-3-3 sigma protein (26-30kD) |
14-3-3 protein σ |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, glycerol, azide, affinity purified) |
IP, WB |
Human, Mouse, Rat |
immunogen = GST fusion protein corresponding to full-length human 14-3-3 sigma |
14-3-3 protein σ; no cross-reaction with other 14-3-3 isoforms |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, serum) |
WB |
Mammal (various), Bovine, Rabbit, Chicken, Sheep, Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to acetylated N-terminal sequence of sheep 14-3-3 sigma |
14-3-3 protein σ (SFN) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, PBS, glycerol, azide, purified) |
ELISA, IP, WB |
Bovine, Human, Mouse, Rat |
immunogen = recombinant 14-3-3 theta/tau (a single protein which is known by the alternative names 14-3-3θ and 14-3-3τ) |
14-3-3 proteinθ/τ; no cross-reaction with 14-3-3ζ |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
|
ELISA, WB |
Bovine, Human, Mouse, Rat |
- |
- |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(lyophilized, affinity purified) |
IH, WB |
Dog, Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to a region of human 14-3-3τ (YWHAQ) |
14-3-3τ (YWHAQ) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, glycerol, no preservative, affinity purified) |
IP, WB |
Human |
immunogen = recombinant protein of human 14-3-3 gamma (26-33kD) |
recognizes beta, gamma, sigma and zeta isoforms of 14-3-3 |
- |
|
|
Novus Biologicals |
monoclonal
IgG1,κ |
Mouse
(liquid, ascites) |
WB |
Human |
immunogen = an N-terminal fragment of human 14-3-3 gamma |
specific for human non-modified N-terminus 14-3-3 gamma; naturally occurring form of 14-3-3 gamma in which the N-terminus Met is removed and the Val is acetylated isnot recognized by this antibody |
- |
|
|
Novus Biologicals |
monoclonal
IgG2a |
Mouse
(liquid, ascites) |
WB |
Human |
immunogen = an N-terminal fragment of human 14-3-3 gamma where the N-terminal Val was acetylated |
specific for human 14-3-3 gamma; where the N-terminal Met is removed, resulting in an acetylated N-terminal Val. Unprocessed (non-modified) 14-3-3 gamma is not recognized by this antibody |
- |
|
|
US Biological |
monoclonal
IgG1 |
Mouse
(liquid, ascites, HEPES, NaCl, BSA, glycerol, azide) |
ELISA, IP, WB |
- |
immunogen = synthetic phospho-(Ser) 14-3-3 binding motif peptides (KLH coupled) |
wide range of peptides containing this phosphorylated 14-3-3 binding motif; no cross-reaction with corresponding non-phosphorylated sequences |
- |
|
|
US Biological |
polyclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
ELISA, IH, IP, WB |
- |
immunogen = synthetic phospho-(Ser) 14-3-3 binding motif peptides (KLH coupled) |
binds peptides and proteins containing a motif composed of phospho-Ser with proline at the +2 position and arginine or lysine at the -3 position; no cross-reaction with corresp. non-phosphorylated sequences or with other phospho-Thr/Ser/Tyr -containing motifs |
- |
|
|
US Biological |
monoclonal
IgG2a, κ |
Mouse
(liquid, PBS, azide, protein G affinity purified) |
ELISA, WB |
Human |
immunogen = recombinant human 14-3-3 epsilon (1-255aa) purified from E. coli |
wide range of peptides containing this phosphorylated 14-3-3 binding motif; no cross-reaction with corresponding non-phosphorylated sequences |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgY |
Chicken
(liquid, PBS, no preservative, affinity purfied) |
WB |
Bovine, Human, Mouse, Rat |
immunogen = recombinant protein from human YWHAE (aa1-255) |
14-3-3 protein epsilon (YWHAE) |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgY |
Chicken
(liquid, PBS, no preservative, affinity purified) |
WB |
Human, Mouse, Rat |
immunogen = recombinant protein corresp. to aa1-248 from human SFN |
14-3-3 protein σ; (Stratifin) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG |
Mouse
(liquid, PBS, BSA, glycerol, azide, affinity purified) |
IF, IH, WB |
Dog, Rabbit, Human, Monkey, Mouse, Rat |
- |
recognizes a ~31 kDa protein, corresponding to the apparent molecular weight of the beta and zeta isoforms of 14-3-3 |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, no preservative, no BSA, protein G purified) |
IC, IF, IP, WB |
Human, Rat |
immunogen = recombinant full length human 14-3-3 gamma protein |
14-3-3 protein (beta, gamma and zeta isoforms) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum, PBS, azide, BSA, thimerisol) |
IP, WB |
Human, Mouse, Rat |
immunogen = a mixture of 14-3-3 fusion proteins (beta, eta, tau, and sigma) |
14-3-3 isoforms |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin, affinity purified) |
IH, IP, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide with the sequence D K S E L V Q K A K L A E Q A E R Y D, mapping to the N-terminal domain of human 14-3-3 |
14-3-3 isoforms (alpha, beta, zeta, and theta) |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, HEPES, NaCl, glycerol, BSA, no preservative, protein A purified) |
ELISA, IP, WB |
- |
immunogen = synthetic phospho(Ser) 14-3-3 binding motif peptides (KLH coupled) |
binds peptides/ proteins containing phospho-Ser surrounded by Pro at the +2 position and Arg/Lys at the -3 position. No crossreactivity is observed with corresp. nonphosphorylated seq. |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
ELISA, WB |
Cow, Human, Mouse, Rat, Xenopus, C. elegans |
immunogen = 20 aa synthetic peptide derived from the N-terminus of the human 14-3-3 beta/alpha protein |
broadly reactive with members of the 14-3-3 family of proteins (~29–32kDa) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, HEPES, BSA, NaCl, no preservative, glycerol, affinity purified) |
WB |
Human, Rat |
immunogen = phosphopeptide corresp. to aa residues surrounding the phosphorylation site Ser58 of rat 14-3-3 protein |
specific for the ~29k 14-3-3 protein phosphorylated at Ser58 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, HEPES, BSA, NaCl, no preservative, glycerol, affinity purified) |
WB |
Human, Mouse, Rat, Xenopus |
immunogen = synthetic peptide corresp. to aa residues from the C-terminal region of human 14-3-3 protein, conj. to KLH |
14-3-3 protein |
- |
|
|
Abcam |
polyclonal
IgY |
Chicken
(liquid, PBS, no preservative, affinity purified) |
WB |
Human, Mouse, Rat |
immunogen = full length E coli-derived fusion protein |
14-3-3 protein |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, BSA, azide, protein G purified) |
IP, WB |
Human |
immunogen = recombinant full length human 14-3-3 gamma protein |
14-3-3 protein |
- |
|
|
Abcam |
polyclonal
IgG |
Sheep
(liquid, PBS, azide, protein G purified) |
WB |
Human |
immunogen = full length native 14-3-3 protein purified from human brain |
recognises the lower molecular weight subunit (26 kDa) of 14-3-3 |
- |
|
|
Abcam |
monoclonal
IgG2a |
Mouse
(liquid, supernatant, no preservative) |
WB |
Rat |
immunogen = synthetic peptide, corresp. to aa208-244 of rat 14-3-3 |
14-3-3 protein (33 kDa) |
- |
|
|
Abcam |
monoclonal
IgG |
Rabbit
(liquid, tris-glycine, NaCl, BSA, glycerol, azide) |
FC, IC, IH, IP, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to residues in the C-terminal of human 14-3-3 alpha + beta |
specific for 14-3-3 alpha + beta |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, no preservative, sucrose, affinity purified) |
IH, WB |
Human |
immunogen = synthetic peptide, corresp. to a region between aa71-120 of human 14-3-3η |
14-3-3eta (28kDa) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, NaCl, azide, glycerol, affinity purified) |
ELISA, IC, IF, IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide derived from internal of human 14-3-3 gamma |
detects endogenous levels of total 14-3-3 gamma protein |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, NaCl, azide, affinity purified) |
ELISA, IC, IF, IH, WB |
Human, Mouse |
immunogen = synthetic non-phosphopeptide derived from human 14-3-3 zeta + delta around the phosphorylation site of threonine 232 (S-D-TP-Q-G) |
detects endogenous levels of total 14-3-3 zeta + delta protein. |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, protein G purified) |
ELISA, IH, WB |
Human |
immunogen = synthetic peptide selected within aa 200~300 of human 14-3-3 beta, conj. KLH |
binds both transcript variants of 14-3-3 (alpha and beta) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, protein G purified) |
ELISA, IH, WB |
Human |
immunogen = synthetic peptide selected within aa 50~150 of human 14-3-3 beta (N-terminal), conj. KLH |
binds to both isoforms of 14-3-3 (alpha and beta) |
- |
|
|
Abcam |
monoclonal
IgG |
Rabbit
(liquid, Ig fract., tris-glycine, NaCl, BSA, glycerol, azide) |
FC, IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to residues in the N-terminal of human 14-3-3 alpha + beta |
specific for 14-3-3 alpha + beta |
- |
|
|
Abcam |
monoclonal
IgG |
Rabbit
(liquid, PBS, PMSF, glycerol, no preservative, protein G purified) |
IC, IH, WB |
Dog, Rabbit, Cow, Sheep, Pig, Human, Monkey, Mouse, Rat |
immunogen = lysed transformed amnion cells (AMA) (human) |
specific for 14-3-3 alpha + beta |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, HEPES, BSA, NaCl, no preservative, glycerol, protein A purified) |
ELISA, IH, IP, WB |
- |
immunogen = synthetic phospho (Ser) 14-3-3-binding-motif peptides |
binds peptides and proteins containing a motif composed of phospho-Ser with proline at the +2 position and arginine or lysine at the -3 position |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum) |
ELISA, IH, WB |
Rabbit, Chicken, Cow, Sheep, Human, Mouse, Rat |
immunogen = synthetic peptide from within N-terminus of 14-3-3 epsilon |
14-3-3epsilon; may not react with recombinant protein expressed in E. coli that is not N-acetylated |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified) |
ELISA, IC, IF, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide (human) from an internal region |
detects endogenous levels of total 14-3-3eta |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, no preservative, protein G purified) |
IP, WB |
Human |
immunogen = recombinant full length human 14-3-3 gamma protein |
14-3-3γ; no crossreactivity with other isoforms of 14-3-3 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, NaCl, glycerol, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
immunogen = synthetic phosphopeptide from human 14-3-3-zeta, around the phosphorylation site of serine 58 (R-S-pS-W-R) |
endogenous levels of 14-3-3 zeta only when phosphorylated at serine 58 (28kDa) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, protein G purified) |
ELISA, IH, WB |
Human |
immunogen = synthetic peptide selected within aa 200-300 of human 14-3-3 zeta (C-terminal) |
specific for 14-3-3 zeta + delta |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified) |
ELISA, IH, WB |
Human, Mouse, Rat |
immunogen = synthesized non-phosphopeptide derived from human 14-3-3 zeta around the phosphorylation site of serine 58 (R-S-Sp-W-R) |
endogenous levels of total 14-3-3 zeta protein |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, BSA, affinity purified) |
IH, IP, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide (unfortunately, the aa seq. is considered to be commercially sensitive) (human) (N-terminal) |
14-3-3beta |
- |
|
|
Abcam |
monoclonal
IgG2b, κ |
Mouse
(liquid, PBS, no preservative, glycerol, protein A purified) |
ELISA, WB |
Human, Mouse, Rat |
immunogen = recombinant full length human protein |
14-3-3beta |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, NaCl, azide, affinity purified) |
ELISA, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide from C-terminus of human 14-3-3 epsilon |
detects endogenous levels of total 14-3-3 epsilon protein |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum) |
ELISA, IH, WB |
Rabbit, Chicken, Cow, Sheep, Human, Mouse, Rat |
immunogen = synthetic peptide from C-terminus of 14-3-3 epsilon |
14-3-3epsilon |
- |
|
|
Abcam |
polyclonal
IgY |
Chicken
(liquid, PBS, no preservative, affinity purified) |
WB |
Cow, Human, Mouse, Rat |
immunogen = full length protein human 14-3-3 epsilon |
14-3-3epsilon |
- |
|
|
Abcam |
monoclonal
IgG2a, κ |
Mouse
(liquid, PBS, azide, protein G purified) |
ELISA, WB |
Human |
immunogen = recombinant full length human protein purified from E. coli |
14-3-3epsilon |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, no BSA, glycerol, no preservative protein G purified) |
IC, IF, IH, IP, WB |
Human |
immunogen = recombinant full length human 14-3-3σ |
specific to 14-3-3 sigma; shows no cross-reaction with other isoforms of 14-3-3 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole serum, azide) |
IH |
Human |
immunogen = synthetic peptide: S N E E G S E E K G P E V, corresp. to internal seq. aa69-81 of human 14-3-3 sigma |
specific to 14-3-3 sigma |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, NaCl, azide, glycerol, affinity purified) |
ELISA, WB |
Human, Mouse |
immunogen = synthetic peptide human 14-3-3 sigma |
detects endogenous levels of total 14-3-3 sigma protein |
- |
|
|
Abcam |
polyclonal
IgG |
Goat
(liquid, TBS, BSA, azide, affinity purified) |
ELISA, WB |
Human |
immunogen = synthetic peptide: D L H T L S E D S Y K D S T from the internal region of human 14-3-3 sigma protein seq. (NP_006133.1) |
14-3-3 sigma protein |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, BSA, glycerol, azide, protein G purified) |
IC, IF, IH, IP, WB |
Human, Mouse |
immunogen = recombinant full length protein (human) |
recognizes human 14-3-3 sigma protein, 26-30kD; no crossreaction with other isoforms of 14-3-3 |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, Tris-glycine, NaCl, glycerol, azide, protein G purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = recombinant full length human 14-3-3σ |
recognizes 14-3-3 sigma and does not crossreact with other 14-3-3 isoforms. It has been specifically tested against 14-3-3 gamma, beta, zeta, and epsilon |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, BSA, azide, protein G purified, prediluted) |
IH |
Human |
immunogen = recombinant human 14-3-3σ |
specific to 14-3-3 sigma |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, PBS, glycerol, azide, protein G purified) |
IP, WB |
Cow, Human, Mouse, Rat |
immunogen = recombinant full length protein (human) |
recognises 14-3-3-theta/tau isoform; no reaction with the 14-3-3 zeta isoform |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, azide, NaCl, affinity purified) |
ELISA, IC, IF |
Human, Mouse |
immunogen = synthetic phosphopeptide derived from human 14-3-3 Tau around the phosphorylation site of serine 232 (S-D-Sp-A-G) |
detects 14-3-3 Tau only when phosphorylated at serine 232 |
- |
|
|
Abcam |
polyclonal
IgG |
Mouse
(liquid, whole serum, glycerol, no preservative) |
WB |
Mouse |
immunogen = vector coding for a partial recombinant fusion protein, corresp. to aa51-151 of mouse 14-3-3 Tau |
detects 14-3-3 Tau |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, no preservative, glycerol, protein A purified) |
WB |
Human, Mouse, Rat |
immunogen = recombinant full length protein (human) |
14-3-3beta |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, no preservative, no BSA, protein G purified) |
IP, WB |
Human |
immunogen = recombinant full length human 14-3-3 gamma protein |
14-3-3γ; no crossreactivity with other isoforms of 14-3-3 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum, azide) |
ELISA, IH, WB |
Chicken, Cow, Sheep, Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to acetylated N- terminal seq. of sheep 14-3-3 Tau |
recognises 14-3-3 tau; may not react with recombinant proteins that are not N-acetylated |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, NaCl, azide, affinity purified) |
ELISA, IC, IF |
Human, Mouse |
immunogen = synthetic non-phosphopeptide derived from human 14-3-3 Tau near phosphorylation site of serine 232 (S-D-Sp-A-G) |
recognises 14-3-3 tau; may not react with recombinant proteins that are not N-acetylated |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, NaCl, azide, affinity purified) |
ELISA, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide derived from internal sequence of human 14-3-3 Tau |
detects endogenous levels of total 14-3-3 Tau protein |
- |
|
|
Abcam |
polyclonal
IgG |
Goat
(liquid, TBS, BSA, azide, affinity purified) |
ELISA, WB |
Human |
immunogen = Synthetic peptide: C-D D R K Q T I D N S Q, corresp. to internal seq. aa136-146 of human 14-3-3 Tau (NP_006817.1) |
detects endogenous levels of total 14-3-3 Tau protein |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, NaCl, azide, glycerol, affinity purified) |
ELISA, IH |
Human, Mouse |
immunogen = synthetic phosphopeptide derived from human 14-3-3 zeta + delta around the phosphorylation site of threonine 232 (S-D-Tp-Q-G) |
detects endogenous levels of 14-3-3 zeta + delta only when phosphorylated at threonine 232 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide: A G D D K K G I V D Q S Q Q A Y, corresp. to aa134 - 149 of human 14-3-3 zeta |
specific for 14-3-3 zeta (30kDa) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified) |
ELISA, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide from an internal region of human 14-3-3 zeta |
specific for 14-3-3 zeta (30kDa) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
ELISA, WB |
Cow, Human, Mouse, Rat, Xenopus, C. elegans |
immunogen = synthetic peptide derived from the N-terminus of human 14-3-3 alpha/beta protein |
broadly reactive with the ~29-32 kDa members of the 14-3-3 family of proteins |
- |
|
|
Abcam |
monoclonal
IgG |
Mouse
(liquid, PBS, no preservative, glycerol, PMSF, protein G purified) |
IC, WB |
Dog, Cow, Sheep, Pig, Human, Monkey, Mouse, Rat |
immunogen = tissue / cell preparation (lysed transformed human amnion cells) |
14-3-3beta/zeta |
- |
|
|
Abcam |
monoclonal
IgG2b, κ |
Mouse
(liquid, PBS, azide, sucrose, protein A purified) |
IP, WB |
Mouse, Rat |
immunogen = recombinant full length rat protein |
14-3-3beta |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, BSA, affinity purified, prediluted) |
IH |
Human, Mouse, Rat |
immunogen = synthetic peptide (unfortunately, the aa seq. is considered to be commercially sensitive) (human) (N-terminal) |
14-3-3beta |
- |
|
|
Abcam |
polyclonal
IgG2a, κ |
Rabbit
(liquid, PBS, no preservative, protein G purified) |
WB |
Human |
immunogen = recombinant fragment corresp. to aa71-170 of human 14-3-3 eta |
14-3-3eta |
- |
|
|
Abcam |
monoclonal
IgG2b, κ |
Mouse
(liquid, PBS, no preservative, protein G purified) |
WB |
Human |
immunogen = recombinant fragment corresp. to aa70-170 of human 14-3-3 gamma |
14-3-3γ |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum, no preservative) |
ELISA, WB |
Chicken, Cow, Sheep, Human, Mouse, Rat |
immunogen = synthetic peptide from the N-terminal area of sheep 14-3-3 gamma |
14-3-3 gamma protein |
- |
|
|
Abcam |
monoclonal
IgG, κ |
Mouse
(liquid, PBS, BSA, azide, protein G purified) |
IP, WB |
Human |
immunogen = recombinant full length human 14-3-3 gamma protein |
recognizes both zeta and gamma isoforms. This antibody shows no crossreactivity with other isoforms of 14-3-3 |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, glycerol, azide, PBS, conj. agarose) |
IP, WB |
Cow, Human, Mouse, Rat |
immunogen = recombinant full length protein (human) |
reacts specifically with the Tau + Theta isoform and does not react with 14-3-3 zeta |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, glycerol, azide, PBS, conj. HRP) |
IP, WB |
Cow, Human, Mouse, Rat |
immunogen = recombinant full length protein (human) |
reacts specifically with the Tau + Theta isoform and does not react with 14-3-3 zeta |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
WB |
Human, Mouse |
immunogen = recombinant frag., corresp. to N-terminal aa1-150 of 14-3-3 zeta |
specific for 14-3-3 zeta + delta |
- |
|
|
Abcam |
polyclonal
IgG |
Mouse
(liquid, whole serum, glycerol, no preservative) |
WB |
Mouse |
immunogen = recombinant fusion protein corresp. to aa51-150 of mouse 14-3-3 zeta |
specific for 14-3-3 zeta (30kDa) |
- |
|
|
Abcam |
monoclonal
IgG2a |
Mouse
(liquid, ascites) |
WB |
Sheep, Rat |
immunogen = tissue / cell preparation (sheep pineal gland) |
14-3-3 zeta |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified, prediluted) |
IH |
Human, Mouse, Rat |
immunogen = synthetic peptide: A G D D K K G I V D Q S Q Q A Y, corresp. to aa134 - 149 of human 14-3-3 zeta |
specific for 14-3-3 zeta |
- |
|
|
Abcam |
polyclonal
IgG |
Sheep
(liquid, BSA, azide, glycerol, TBS, affinity purified) |
WB |
Cow, Sheep, Human, Mouse, Rat |
immunogen = synthetic phosphopeptide corresp. to aa surrounding phospho Serine185 |
recognizes 14-3-3 phospho Serine185. It is specific for the phosphorylated form of beta and zeta 14-3-3 that are found endogenously phosphorylated only in brain tissue |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole serum, no preservative) |
IH, WB |
Mammal (various) |
immunogen = Synthetic peptide: K M K G D Y Y R Y L A E, corresp. to aa124-135 of 14-3-3 eta + gamma + zeta |
recognizes eta, gamma and zeta isoforms at 27kDa |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole serum, no preservative) |
WB |
Various vertebrate |
immunogen = HPLC purified synthetic peptide (K M K G D Y Y R Y L A E) corresp. to the zeta, gamma and eta isoforms of 14-3-3 |
recognizes eta, gamma and zeta isoforms |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole serum, no preservative) |
WB |
Rabbit, Chicken, Cow, Sheep, Human, Mouse, Rat |
immunogen = synthetic peptide from the N-terminal area of sheep 14-3-3 eta |
14-3-3eta |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, IF, WB |
Arabidopsis thaliana |
immunogen = epitope mapping near the C-terminus of Arabidopsis thaliana 14-3-3 |
14-3-3 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin) |
ELISA, IF, IP, WB |
Arabidopsis thaliana |
immunogen = epitope corresp. to aa 21-75 mapping within an internal region of Arabidopsis thaliana 14-3-3 |
14-3-3 and to a lesser extent 14-3-3-like proteins |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = raised against aa 1-246 representing full length human 14-3-3β |
14-3-3 |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = raised against aa 1-246 representing full length human 14-3-3β |
14-3-3 |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = raised against aa 1-246 representing full length human 14-3-3β |
14-3-3 protein family |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, FC, IF, IH, IP, WB |
Human, Mouse, Rat |
immunogen = epitope mapping at the N-terminus of human 14-3-3β |
detects members of 14-3-3 protein family |
- |
|
|
Santa Cruz |
monoclonal
IgG2b |
Mouse
(liquid, PBS, azide, gelatin) |
IP, WB |
Mouse, Rat |
immunogen = raised against recombinant rat 14-3-3β |
14-3-3β |
- |
|
|
Santa Cruz |
monoclonal
IgG2b |
Mouse
(liquid, PBS, azide, gelatin) |
IP, WB |
Human, Mouse, Rat |
immunogen = raised against recombinant human 14-3-3β |
14-3-3β |
- |