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Antibodies

7 Results

Name Source Clonality &
Isotype		 Host &
Formulation		 Method Reactivity Immunogen/
Epitope		 Specificity Availability
Microglial Markers From manufacturers (6)
Abcam monoclonal
IgG2b		 Mouse
(liquid, PBS, BSA, azide, affinity purified; conj. FITC)		 FC Human, Rat immunogen = information is considered commercially sensitive allograft inflammatory factor-1 (AIF1) or ionized calcium-binding adaptor molecule 1 (Iba1) -
Abcam monoclonal
IgG2b		 Mouse
(liquid, BSA, PBS, azide, protein G purified)		 IH Human immunogen = recombinant full length protein (human) allograft inflammatory factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) -
Abcam polyclonal
IgG		 Goat
(liquid, tris-saline, BSA, azide, affinity purified)		 IF, IH, WB Human, Rat immunogen = synthetic peptide: T G P P A K K A I S E L P, corresp. to aa 81-93 of human AIF 1 Allograft Inflammatory Factor-1 (AIF1) or ionized calcium-binding adaptor molecule 1 (Iba1); recognise the isoforms represented by NP_001614 and NP_116573 but not NP_004838 -
Biocare Medical polyclonal
 Rabbit
(liquid, concentrated, buffer with protein carrier and preservative.Also available prediluted, ready to use as PP290 AA)		 IH Human, Mouse, Rat Immunogen = Iba1 detects Iba-1 in cytoplasm of microglia and macrophages -
Wako Chemicals polyclonal
 Rabbit
(sera, affinity purified, TBS, no preservatives or stabilizers)		 WB Human, Mouse, Rat Immunogen = synthetic peptide corresponding to the C-terminus of Iba-1 (Ionized calcium binding adaptor molecule 1) Microglia and macrophages, but no cross reactivity with neurons and astrocytes -
Wako Chemicals polyclonal
 Rabbit
(sera, affinity purified, TBS, no preservatives or stabilizers)		 IC Human, Mouse, Rat Immunogen = synthetic peptide corresponding to the C-terminus of Iba-1 (Ionized calcium binding adaptor molecule 1) Microglia and macrophages, but no cross reactivity with neurons or astrocytes -
Mitochondrial Proteins, Microglial Markers From manufacturers (1)
Abcam
Cat# ab109497		 monoclonal
IgG		 Rabbit
(Tissue culture supernatant. PBS 49 percent, sodium azide 0.01 percent, glycerol 50 percent, BSA 0.05 percent.)		 FC, IC, IH, IP, WB Human, Mouse A synthetic peptide corresponding to residues at the C-terminus of human peripheral benzodiazepine receptor (PBR)/translocator protein (TSPO). This antibody recognizes PBR, also known as TSPO, a mitochondrial transporter that is upregulated in activated microglia. Confirmed available December 2014.