γ-tubulin (GTU-88)
Novus Biologicals
monoclonal
IgG1
Mouse
(liquid, ascites, azide)
-
Dog, Chicken, Cow, Human, Hamster, Mouse, Rat, Xenopus
immunogen = synthetic peptide corresp. to N-terminal aa 38-53 of γ-tubulin, conj. to KLH
γ-tubulin; location centrosomal
-
γ-Tubulin
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, purified, PBS, azide)
-
Human, Mouse, Rat
immunogen = synthetic peptide: D E Y H A A T R P D Y I S W G T Q E Q-K, with N-terminally added lysine corresp. to aa 433-451 of γ-Tubulin, conj. to KLH,
centrosome marker; recognizes 48 kDa γ-tubulin; does not react with α, β, ω or η tubulin
-
ε-Tubulin (TUB-11)
Novus Biologicals
monoclonal
IgG2a
Mouse
(liquid, protein-A purified, PBS, BSA, azide)
-
Chicken, Frog, Human, Mouse
immunogen = synthetic peptide conj. to KLH, corr. to aa 352-366 of human ε-Tubulin
recognizes 55 kDa ε-tubulin
-
Tubulin (YL1/2)
Novus Biologicals
monoclonal
IgG2a
Rat
(liquid, glycerol, azide)
-
Mammal (various), Allium, Slime molds, S. cerevisiae, S. pombe
immunogen = full length native protein (purified) (S. cerevisiae)
detects the tyrosinated form of the alpha-tubulin subunit
-
Tubulin (YOL1/34)
Novus Biologicals
monoclonal
IgG2a
Rat
(liquid, glycerol, azide)
-
Mammal (various), Allium, Slime molds, S. cerevisiae, S. pombe
immunogen = full length native protein (purified) (S. cerevisiae
detects an epitope on α-tubulin between the aa 414-422
-
γ-Tubulin (C-20)
Santa Cruz
polyclonal
IgG
Goat
(affinity purified, PBS, gelatin, azide; also FITC, AC or HRP conj.)
-
Human, Mouse, Rat, Xenopus
carboxy terminus of gamma tubulin of human origin
gamma tubulin only; no cross with other tubulins
-
βIII-Tubulin TuJ1
Sigma Aldrich
polyclonal
IgG
Rabbit
(liquid, affinity isolated, PBS, BSA, azide)
-
Human, Mouse, Rat
immunogen = synthetic peptide corr. to aa residues 441-450 of human β-tubulin III with N-terminal added cysteine, conjugated to KLH
~55 kDa β-Tubulin III, in WB detectable in human white blood cells or platelets
-
α-Tubulin (DM 1A)
Seikagaku
monoclonal
Mouse
(liquid, ascites fluid, azide)
-
Amphibian (various), Bovine, Chicken, Fungi, Yeast, Human, Mouse, Rat
immunogen = purified chick brain microtubules
specific for alpha tubulin
-
α-Tubulin (DM1A)
Upstate and Chemicon are Millipore
monoclonal
IgG1
Mouse
(liquid, protein G purified, PBS, BSA, tween-20, azide; conj. FITC and Alexa Fluor® 555)
-
Avian (various), Bovine, Pig, Human, Guinea Pig, Mouse, Rat
immunogen = purified human brain α-Tubulin
α-Tubulin
-
α-Tubulin (DM1A)
Upstate and Chemicon are Millipore
monoclonal
IgG1
Mouse
(liquid, protein G purified, PBS, BSA, tween-20, azide, conj. Alexa Fluor488 and FITC)
-
Avian (various), Bovine, Pig, Human, Guinea Pig, Rat
immunogen = purified human brain α-Tubulin
reacts with the aa seq. 426-450 on alpha-tubulin (A A L E K D Y E E V G V D S V E G E G E E E G E E)
-
α-Tubulin (DM1A)
Upstate and Chemicon are Millipore
monoclonal
IgG1
Mouse
(liquid, protein G purified, PBS, glycerol, azide)
-
Avian (various), Bovine, Pig, Human, Guinea Pig, Mouse, Rat
immunogen = purified human brain α-Tubulin
α-tubulin; epitope recognized by this antibody has been mapped to amino acids 426-450
-
βIII-Tubulin (TUJ1 1-15-79)
None
monoclonal
IgG
Rabbit
(liquid, PBS, thimerosal, purified)
-
Mammal (various)
immunogen = microtubules derived from rat brain
reactive to neuron specific class III β-tubulin (βIII), but does not identify β-tubulin found in glial cells
Discontinued
βIII-Tubulin
BioLegend
polyclonal
Rabbit
(affinity purified, PBS, thimerosal, glycerol)
-
Mammal (various)
immunogen = microtubules derived from rat brain; same epitope as clone TUJ1
reactive to neuron specific Beta-tubulin (Beta III), not Beta-tubulin found in glial cells.
Available from BioLegend
β-Tubulin (TUB 2.1)
Sigma Aldrich
monoclonal
IgG1
Mouse
(liquid, PBS, azide, purified)
-
Plant (various), Bovine, Rabbit, Chicken, Frog, Sea Urchin, Wheat, Human, Hamster, Mouse, Rat, Moth
immunogen = tubulin from rat brain (epitope in the carboxy-terminal part of β-tubulin between aa 281-446)
reacts with beta tubulin; recognizes all five isoforms of β-tubulin (β1-β5); reacts with the β-Lc and β-Sc fragments in the C-terminal part of β−tubulin in immunoblotting
-
Tubulin, Tyrosine (TUB-1A2)
Sigma Aldrich
monoclonal
IgG3
Mouse
(liquid, ascites, azide)
-
Bovine, Dog, Marsupial, Yeast, Monkey, Mouse, Xenopus
immunogen = peptide containing the carboxy terminal amino acids of α-tubulin
tyrosinated tubulin
-
α-Tubulin GLU
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(affinity purified Ig, liquid, PBS)
-
Mammal (various), Plant (various), Sea Urchin
immunogen = peptide corr. to the C-terminal of Glu tubulin
recognizes the detyrosinated form (Glutubulin) of the tubulin alpha chain
-
α-Tubulin (B-5-1-2)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
-
Various species
immunogen = Sarkosyl resistantribbons from sperm axonemes of Strongylocentrotus purpuratus(sea urchin) origin
alpha tubulin specific
-
α-Tubulin, acetylated (6-11B-1)
Santa Cruz
monoclonal
IgG2b
Mouse
(liquid, PBS, azide, gelatin)
-
Mammal (various), Zebra fish, Xenopus, Drosophila
immunogen = the outer arms of Strongylocentrotus purpuratus (sea urchin) spermaxonemes
alpha tubulin, 55 kDa
-
α Tubulin (YOL 1/34)
Chemicon and Upstate are Millipore
monoclonal
IgG2a
Rat
(liquid, purified, PBS, BSA, azide)
-
Mammal (various), Allium, Eukaryote, Yeast, Human, Slime molds
recognizes the aa seq. 414-422 on alpha-tubulin.
-
α-Tubulin (B-5-1-2)
Sigma Aldrich
monoclonal
IgG1
Mouse
(liquid, ascites, azide)
-
Bovine, Chicken, Sea Urchin, Chlamydomonas, Human, Monkey, Mouse, Rat
immunogen = Sarkosyl- resistant filaments from sea urchin sperm axonemes
recognizes the C-terminal end of the alpha-tubulin isoform
-
α-Tubulin, acetylated (6-11B-1)
Sigma Aldrich
monoclonal
IgG2b
Mouse
(liquid, ascites, azide)
-
Invertebrate (various), Plant (various), Bovine, Chicken, Frog, Pig, Human, Monkey, Hamster, Mouse, Rat
immunogen = acetylated tubulin from the outer arm of sea urchin
reacts with acetylated α-tubulin, epitope located on the α3 isoform
-
α-Tubulin, acetylated (6-11B-1)
Sigma Aldrich
monoclonal
IgG2b
Mouse
(liquid, PBS, azide, purified)
-
Invertebrate (various), Plant (various), Bovine, Chicken, Frog, Pig, Human, Hamster, Mouse, Rat
immunogen = acetylated tubulin from the outer arm of sea urchin
reacts with acetylated α-tubulin, epitope located on the α3 isoform
-
β-Tubulin (9F3)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, azide)
-
Bovine, Zebra fish, Human, Monkey, Mouse, Rat, Drosophila
immunogen = a synthetic peptide corresponding to the amino terminus of human β-tubulin
detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin
-
β-Tubulin (9F3)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, PBS, sodium azide, BSA, Alexa Fluor® 555 conj.)
-
Bovine, Zebra fish, Human, Monkey, Mouse, Rat, Drosophila
immunogen = a synthetic peptide corresponding to the amino terminus of human β-tubulin
recognizes endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin
-
β-Tubulin (9F3)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, PBS, sodium azide, BSA, Alexa Fluor® 647 conj.)
-
Bovine, Zebra fish, Human, Monkey, Mouse, Rat, Drosophila
immunogen = a synthetic peptide corresponding to the amino terminus of human β-tubulin
recognizes endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin
-
β-Tubulin (9F3)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, PBS, sodium azide, BSA, Alexa Fluor® 488 conj.)
-
Bovine, Zebra fish, Human, Monkey, Mouse, Rat, Drosophila
immunogen = a synthetic peptide corresponding to the amino terminus of human β-tubulin
recognizes endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin
-
β-Tubulin (9F3)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, saline, KCl, sodium phosphatedibasic, BSA, glycerol)
-
Bovine, Zebra fish, Human, Monkey, Mouse, Rat
immunogen = a synthetic peptide corresponding to the amino terminus of human β-tubulin
detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin
-
β-Tubulin
Cell Signaling
polyclonal
Rabbit
(liquid, HEPES, saline, BSA, glycerol)
-
Bovine, Zebra fish, Human, Monkey, Mouse, Rat
immunogen = a synthetic peptide corresponding to the sequence of human β-tubulin
detects endogenous levels of total β-tubulin protein, and does not cross-react with recombinant α-tubulin
-
β3-Tubulin (TU-20)
Cell Signaling
monoclonal
IgG1
Mouse
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Human, Mouse, Rat
immunogen = a synthetic peptide corresponding to the carboxy terminus of human β3-tubulin protein
Recognizes endogenous levels of total β3-tubulin protein and does not cross-react with tubulin isoforms expressed in non-neuronal cells. This clone is similar to TUJ1
-
Tubulin α
Anaspec
polyclonal
Rabbit
(liquid, affinity purified, PBS, BSA, azide)
-
Chicken, Frog, Cow, Pig, Human, Gerbil, Guinea Pig, Mouse, Rat
immunogen = a synthetic peptide derived from amino acids 426-450 of human tubulin α
detects Tubulin α
-
Tubulin γ
Anaspec
polyclonal
Rabbit
(liquid, affinity purified, PBS, BSA, azide)
-
Human
immunogen = a synthetic peptide corresponding to the amino acids 432-451 of human Tubulin-γ protein
detects Tubulin γ
-
Tubulin β
Anaspec
polyclonal
Rabbit
(liquid, affinity purified, PBS, BSA, azide)
-
Chicken, Frog, Sea Urchin, Yeast, Cow, Pig, Human, Gerbil, Guinea Pig, Mouse, Rat, Myxamoeba, Plasmodium
immunogen = a synthetic peptide derived from amino acids 416-430 of human tubulin β
detects Tubulin β
-
α-Tubulin (DM1A)
Sigma Aldrich
monoclonal
IgG1
Mouse
(protein-A purified, gel filtered, PBS, BSA, azide, FITC conj.)
-
Amphibian (various), Bovine, Goat, Chicken, Sea Urchin, Fungi, Yeast, Kangaroo, Human, Monkey, Gerbil, Hamster, Mouse, Rat, Nicotiana tabacum, Trypanosome
immunogen = purified chick brain microtubules (epitope in the C-terminal of α-tubulin)
detects the carboxy-terminal part of alpha-tubulin
-
γ-Tubulin (GTU-88)
Sigma Aldrich
monoclonal
IgG1
Mouse
(liquid, PBS, azide, purified)
-
Bovine, Dog, Chicken, Human, Hamster, Mouse, Rat, Xenopus
immunogen = synthetic γ-tubulin peptide (N-terminal aa 38-53) conj. to KLH
reacts with 48 kDa γ-tubulin, N-terminal epitope
-
α-Tubulin (B-5-1-2)
Sigma Aldrich
monoclonal
IgG1
Mouse
(liquid, purified IgG, PBS, azide)
-
Bovine, Chicken, Sea Urchin, Chlamydomonas, Human, Monkey, Mouse, Rat
immunogen = Sarkosyl-resistant filaments from sea urchin
recognizes an epitope located at the C-terminal end of alpha-tubulin
-
β-Tubulin (DM1A)
Calbiochem EMD Biosciences
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Chicken, Human, Gerbil, Mouse, Rat
immunogen = native chick brain microtubules
recognizes the ~60 kDa tubulin protein found in all eukaryotic cells
-
β-Tubulin (DM1B)
Calbiochem EMD Biosciences
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Chicken, Human, Gerbil, Mouse, Rat
immunogen = native chick brain microtubules
recognizes the ~60 kDa tubulin protein found in all eukaryotic cells
-
βIII-Tubulin (5G8)
Promega
monoclonal
IgG1
Mouse
(liquid, PBS, no preservatives)
-
Mammal (various)
immunogen = a peptide (EAQGPK) corresponding to the C-terminus of βIII tubulin
used for labeling neurons in tissue sections and cell culture; does not label nonneuronal cells (e.g., astrocytes)
-
βIII-Tubulin TuJ1
Sigma Aldrich
polyclonal
IgG
Rabbit
(liquid, affinity isolated, PBS, azide)
-
Human, Mouse, Rat
immunogen = synthetic peptide corr. to residues 151-165 of human β-Tubulin III.
~50 kDa β-Tubulin III
-
α/β-Tubulin
Cell Signaling
polyclonal
Rabbit
(liquid, affinity purified, HEPES, saline, BSA, glycerol)
-
Bovine, Zebra fish, Human, Monkey, Mouse, Rat
immunogen = a synthetic peptide corresponding to the sequence of human α- and β-tubulin
detects endogenous levels of both α- and β-tubulin total protein, and does cross-react with both recombinant α- and β-tubulin
-
βIII-Tubulin (TUJ1)
BioLegend
monoclonal
IgG2a
Mouse
(liquid, PBS, thimerosal, affinity purified, conj. AlexaFluor®488)
-
Mammal (various)
immunogen = microtubules derived from rat brain
reactive to neuron specific class III β-tubulin (βIII), but does not identify β-tubulin found in glial cells
Available from BioLegend
γ-Tubulin (GTU-88)
Sigma Aldrich
monoclonal
IgG1
Mouse
(liquid, ascites, azide)
-
Bovine, Dog, Chicken, Human, Hamster, Mouse, Rat, Xenopus
immunogen = synthetic γ-tubulin peptide (N-terminal aa 38-53) conjugated to KLH
reacts with 48 kDa γ-tubulin, N-terminal epitope
-
tubulin-β (E7)
DSHB U. Iowa
monoclonal
IgG1
Mouse
(supernatant or concentrate or ascites)
-
Human, Mouse, Xenopus, Kangaroo rat, Drosophila
immunogen = beta tubulin from E.Coli
detects β-tubulin
-
α-Tubulin (DM1A)
Sigma Aldrich
monoclonal
IgG1
Mouse
(liquid, ascites, azide)
-
Amphibian (various), Bovine, Fungi, Yeast, Human, Mouse
immunogen = purified chick brain tubulin
reacts with the alpha chain of tubulin
-
α-Tubulin (DM1A)
Lab Vision Thermo Scientific
monoclonal
IgG1 ,κ
Mouse
(liquid, ascites, protein G purified, PBS, BSA, azide; available without BSA and azide)
-
Chicken, Frog, Cow, Pig, Human, Gerbil, Guinea Pig, Mouse, Rat
immunogen = native chick brain microtubules; epitope aa426-450
57 kDa of α-tubulin and not β-tubublin
-
βIII-tubulin (TuJ-1)
R&D Systems
monoclonal
IgG2A
Mouse
(supernate, protein-A or G-purified, PBS, trehalose, filtered, lyophilized)
-
Mammal (various), Chicken
immunogen = microtubules derived from rat brain
neuron specific class III β tubulin; not other β-tubulin in glial cells. Stains neuronal cell bodies, dendrites, axons and axonal terminations
-
βIII-tubulin (TuJ-1)
R&D Systems
monoclonal
IgG2A
Mouse
(Protein A or G purified from hybridoma culture supernatant, PBS, BSA, biotinylated, lyophilized)
-
Mammal (various), Chicken
immunogen = microtubules derived from rat brain
detects mammalian and chicken neuronspecific βIII tubulin but not other βtubulin isotypes
-
α-Tubulin (DM1A)
Sigma Aldrich
monoclonal
IgG1
Mouse
(liquid, purified IgG, PBS, BSA, azideAlso available as ascites and as FITC conj.)
-
Bovine, Chicken, Human, Mouse
immunogen = purified chick brain tubulin (epitope at aa 426-430)
recognizes an epitope located at the C-terminal end of alpha-tubulin
-