|
|
Santa Cruz |
monoclonal
IgG2b |
Mouse
(liquid, PBS, gelatin, azide) |
IF, IH, IP, WB |
Human |
aa. 269-451 at the C-terminus of γ tubulin of human origin |
specific for gamma tubulin |
- |
|
|
Santa Cruz |
monoclonal
IgG2a |
Mouse
(liquid, PBS, gelatin, azide) |
IF, IH, WB |
Human, Mouse, Rat |
aa. 269-451 at the C-terminus of γ tubulin of human origin |
specific to gamma tubulin |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(affinity purified IgG, PBS, gelatin, azide) |
IF, WB |
Corn (zea mays), Arabidopsis thaliana, Nicotiana tabacum |
immunogen = peptide mapping at the N-terminus of Arabidopsis thaliana gamma tubulin |
gamma tubulin |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
IF, IP, WB |
Human, Mouse, Rat |
recombinant protein corresponding to aa 269-451 mapping at the C-terminus of human γ-Tubulin |
γ-Tubulin |
- |
|
|
AbD Serotec |
monoclonal
IgG1 |
Mouse
(lliquid, PBS, azide, affinity purified) |
IH, WB |
Rabbit, Pig, Human, Hamster, Mouse |
immunogen = native brain tubulin |
specific for alpha tubulin and recognises an antigenic determinant represented by aa residues 65-79 |
- |
|
|
AbD Serotec |
monoclonal
IgG2a |
Rat
(liquid, supernate, protein-G purified, PBS, azide) |
ELISA, IH, IP, RIA, WB |
Mammal (various), Avian (various), Yeast |
immunogen = yeast tubulin |
specific to Tyr-Tubulin; Gly-Gly-Phe epitope gives some cross with other proteins, including E. coli rec A and oxidized actin |
- |
|
|
AbD Serotec |
monoclonal
IgG2a |
Rat
(liquid, supernate, TRIS/HCl, azide) |
ELISA, IP, RIA |
Mammal (various), Avian (various), Yeast |
immunogen = C-terminus of yeast tubulin |
specific to Tyr-Tubulin; Gly-Gly-Phe epitope gives some cross with other proteins, including E. coli rec A and oxidized actin |
- |
|
|
AbD Serotec |
polyclonal
IgG1 |
Rabbit
(liquid IgG, BSA, PBS, purified, ready to use, azide) |
IH |
Bovine, Pig, Baboon, Human, Hamster, Mouse, Rat |
immunogen = synthetic peptide corresp. to an epitope within the C-terminal region of human class III beta tubulin |
class III beta-tubulin |
- |
|
|
AbD Serotec |
monoclonal
IgM |
Rabbit
(liquid, PBS, azide, purified) |
ELISA, IH, WB |
Plant (various), Pig, Human, Mouse |
immunogen = tubulin beta from porcine brain |
beta-tubulin (N-terminal), 50kDa |
- |
|
|
AbD Serotec |
monoclonal
IgG1 |
Mouse
(liquid IgG, DEAE purified, PBS, azide) |
IH, WB |
Bovine, Porcine, Human, Mouse, Rat, Syrian hamster |
immunogen = synthetic peptide corr. to an epitope within C-terminal region of human class III β-tubulin. |
recognises class III β-tubulin which is reported to be restricted to neuronal tissue |
- |
|
|
AbD Serotec |
monoclonal
IgG1 |
Mouse
(liquid IgG, DEAE purified, PBS, azide, FITC) |
IF |
Cow, Pig, Baboon, Human, Mouse, Rat, Syrian hamster |
immunogen = synthetic peptide corr. to an epitope within C-terminal region of human class III β-tubulin. |
recognises class III β-tubulin which is reported to be restricted to neuronal tissue |
- |
|
|
AbD Serotec |
polyclonal
|
Rabbit
(liquid, purified) |
IB, IH |
Human |
- |
tubulin delta 2 |
- |
|
|
Sigma Aldrich |
monoclonal
IgG2b |
Mouse
(liquid, ascites, azide) |
IF, WB |
Bovine, Chicken, Pig, Human, Mouse, Rat |
immunogen = synthetic peptide corr. to the C-terminal sequence of human β-tubulin isotype II coupled to BSA |
specifically recognizes an epitope located on the isotypes I and II of human β-tubulin.;no reactivity with other tubulin isotypes is observed |
- |
|
|
Sigma Aldrich |
polyclonal
IgG1 |
Rabbit
(affinity purified, gel filtered, PBS, BSA, azide, Cy3 conj.) |
IC |
Chicken, Human |
immunogen = synthetic peptide corr. to the N-terminal region of human γ-tubulin (aa 38-53, with C-terminally added lysine) conjugated to KLH |
γ-tubulin; not other members of the tubulin family |
- |
|
|
Sigma Aldrich |
polyclonal
IgG |
Rabbit
(liquid, affinity isolated, PBS, BSA, azide) |
IB |
Drosophila |
immunogen = synthetic peptide corr. to the C-terminus of Drosophila γ-tubulin (aa 441-457 with N-terminal added cysteine-glycine) |
This sequence is highly specific for Drosophila γ-tubulin and not found in γ-tubulin of other species or other members of the tubulin family such as α-, β-, δ- and ε-tubulins |
- |
|
|
Sigma Aldrich |
polyclonal
IgG |
Rabbit
(liquid, affinity isolated, PBS, azide) |
IB |
C. elegans |
immunogen = synthetic peptide corr. to the C-terminus of C. elegans γ-tubulin (aa 428-444 with N-terminal added cysteine) |
This sequence is highly specific for C. elegans γ-tubulin and not found in γ-tubulin of other species or other members of the tubulin family such as α-, β-, δ- and ε-tubulins |
- |
|
|
Sigma Aldrich |
polyclonal
IgG |
Rabbit
(serum, IgG fraction, azide) |
IC, WB |
Human |
immunogen = synthetic peptide corr. to theN-terminal region of human γ-tubulin (aa 38-53, with C-terminally added lysine) |
specific for N-terminal region of γ-tubulin; epitope not found in α- and β-tubulins |
- |
|
|
Zymed/ Invitrogen |
monoclonal
IgG1,κ |
Mouse
(ascites, protein-A purified, PBS, azide) |
IF, WB |
Human, Mouse, Rat |
immunogen = Sarkosyl-resistant filament from sea urchin sperm axonemes |
recognizes all forms of α-tubulin |
- |
|
|
Zymed/ Invitrogen |
monoclonal
IgG1 |
Mouse
(diluted ascites fluid, azide) |
ELISA, IF, WB |
Various species |
(epitope at aa 65-79) |
α-tubulin, N-terminal structural domain |
- |
|
|
Zymed/ Invitrogen |
monoclonal
IgG1,κ |
Mouse
(ascites, PBS, BSA, azide) |
IH |
Chicken, Human |
immunogen = purified chick brain microtubles |
α-tubulin, cytoplasmic |
- |
|
|
Zymed/ Invitrogen |
monoclonal
IgG2b |
Mouse
(ascites, protein-A purified, PBS, azide) |
IF, WB |
Human, Mouse, Rat |
immunogen = acetylated α-tubulin from the axoneme of sea urchin sperm flagella |
recognizes 55 kDa acetylated form of α-tubulin |
- |
|
|
Zymed/ Invitrogen |
monoclonal
IgG1,κ |
Mouse
(ascites, protein-A purified, PBS, azide) |
IF, WB |
Human, Mouse, Rat, C. elegans |
immunogen = tubulin from sea urchin (S. purpuratus) sperm |
recognizes 50 kDa β-tubulin; binds to the two major and one of the minor β-tubulin isotypes. |
- |
|
|
Zymed/ Invitrogen |
monoclonal
IgG1,κ |
Mouse
(ascites, PBS, BSA, azide) |
IH |
Bovine, Human, Rat |
immunogen = purified rat brain microtubules |
β-tubulin, cytoplasmic |
- |
|
|
Sigma Aldrich |
monoclonal
IgM |
Mouse
(liquid, IgG, PBS, BSA, azide) |
ELISA, WB |
Bovine, Rabbit, Chicken, Sea Urchin, Dinoflagellate, Human, Monkey, Mouse, Rat |
immunogen = purified sea urchin sperm axonemal proteins (epitope in C-terminal region of α- and β-tubulins, the glutamylated motif at amino acid 445-457 of α-tubulin) |
polyglutamylated C-terminal region of α- and β-tubulin |
- |
|
|
AbD Serotec |
polyclonal
IgG |
Rabbit
(liquid, PBS, affinity purified) |
IH, WB |
Mammal (various), Plant (various), Human |
immunogen = synthetic peptide corresp. to the C-terminal portion of detyrosinated tubulin |
recognizes the de-tyrosinated form of the tubulin alpha chain (Glu tubulin). |
- |
|
|
Santa Cruz |
monoclonal
IgG2b |
Mouse
(liquid, PBS, gelatin, azide; also HRP conj. for WB) |
IF, IH, IP, WB |
Human, Mouse, Rat |
aa. 210-444 of β tubulin of human origin |
beta tubulin specific |
- |
|
|
Novus Biologicals |
monoclonal
IgG3 |
Mouse
(liquid, ascites fluid, azide) |
IF, WB |
Bovine, Dog, Marsupial, Yeast, Mouse, Xenopus, African green monkey |
immunogen = synthetic peptide containing the C-terminal aa of α tubulin |
recognizes ~55 kDa tyrosine Tubulin |
- |
|
|
Novus Biologicals |
monoclonal
IgM |
Mouse
(liquid, affinity purified, PBS, BSA, azide) |
IC, WB |
Rabbit, Chicken, Sea Urchin, Cow, Human, Mouse, Rat, Macaque |
immunogen = purified sea urchin --Lytechinus pictus-- sperm axonemal proteins (epitope localized in the C-terminal region of α- and β- tubulins, the glutamylated motif at aa 445-457 of α-tubulin) |
recognizes polyglutamylated tubulin motif at aa 445-457 |
- |
|
|
Biotrend |
monoclonal
IgG1,κ |
Mouse
(supernate) |
IH |
Chicken |
immunogen = native chicken brain microtubules; epitope maps between aa 426-450 |
alpha-tubulin (57kD); no cross reaction with beta-tubulin |
- |
|
|
Biotrend |
monoclonal
IgG1,κ |
Mouse
(supernate, purified, azide) |
IH |
Chicken |
immunogen = native chicken brain microtubules; epitope maps between aa426-450 |
alpha tubulin (54kD); no cross reaction with alpha-tubulin |
- |
|
|
Biotrend |
polyclonal
|
Rabbit
(IgG fraction, affinity purified) |
ELISA, IC, WB |
Human, Mouse, Rat |
- |
beta-tubulin III |
- |
|
|
Biotrend |
monoclonal
IgG1 |
Mouse
(Ig fraction, affinity purified) |
ELISA, IC, WB |
Mouse, Rat |
|
beta-tubulin |
- |
|
|
Biotrend |
polyclonal
|
Rabbit
(Ig fraction, affinity purified) |
ELISA, IC, WB |
Chicken, Human, Mouse, Rat |
|
beta-tubulin phos Ser172 |
- |
|
|
Biotrend |
polyclonal
|
Rabbit
(Ig fraction, affinity purified) |
ELISA, IC, WB |
Chicken, Human, Mouse, Rat |
- |
beta-tubulin (aa168-177) |
- |
|
|
Biotrend |
polyclonal
|
Rabbit
(Ig fraction, affinity purified) |
ELISA, IC, WB |
Human, Mouse, Rat |
|
beta-tubulin, CT |
- |
|
|
Biogenesis AdB Serotec |
polyclonal
IgG |
Rabbit
(liquid, purified) |
IF, WB |
Human |
- |
- |
- |
|
|
Biogenesis AdB Serotec |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, affinity purified, RTU) |
IH |
Bovine, Pig, Baboon, Human, Hamster, Mouse, Rat |
immunogen = beta-III isoform of tubulin, C-terminal sequence |
50 kDa class III beta tubulin |
- |
|
|
Biogenesis AdB Serotec |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, purified, conj. FITC) |
IF |
Bovine, Pig, Baboon, Human, Hamster, Mouse, Rat |
immunogen = beta-III isoform of tubulin, C-terminal sequence |
50 kDa class III beta tubulin |
- |
|
|
Biogenesis AdB Serotec |
monoclonal
IgG1 |
Mouse
(liquid, purified Ig, PBS, azide) |
IH, WB |
Bovine, Pig, Baboon, Hamster, Mouse, Rat |
immunogen = beta-III isoform of tubulin, C-terminal sequence |
50 kDa class III beta tubulin; neuron specific and not in glial cells |
- |
|
|
Biodesign Meridian LifeScience |
monoclonal
IgG1 |
Mouse
(protein-G purified, ascites, PBS, azide) |
ELISA, IF, IH, WB |
Plant (various), Yeast, Various species |
immunogen = alpha tubulin, N-terminal domain (epitope within aa 65-97 of alpha tubulin) |
all alpha-tubulin isoforms |
- |
|
|
Biodesign Meridian LifeScience |
monoclonal
IgG3 |
Mouse
(affinity purified, PBS, BSA, azide) |
IC, WB |
- |
(epitope in carboxyl region of the protein) |
β tubulin; recognizes different epitope from cat# M01123M |
- |
|
|
Biodesign Meridian LifeScience |
monoclonal
IgG3 |
Mouse
(PBS, BSA, EDTA, azide) |
IC, WB |
- |
epitope on beta-tubulin |
β tubulin; recognizes different epitope from cat# M01124M |
- |
|
|
Biodesign Meridian LifeScience |
monoclonal
IgM |
Mouse
(liquid, PBS, azide) |
ELISA, IH, WB |
Plant (various), Pig, Human, Mouse |
immunogen = tubulin beta from porcine brain |
recognises an epitope within the N-terminal region (NT) of tubulin beta (50kDa) |
- |
|
|
Abcam |
monoclonal
IgG3 |
Mouse
(liquid, ascites fluid, azide) |
IF, WB |
Bovine, Dog, Yeast, Mouse, Xenopus, African green monkey |
immunogen = Synthetic peptide containing the carboxy terminal aa's of α tubulin |
recognizes ~55 kDa tyrosine Tubulin |
- |
|
|
Abcam |
monoclonal
IgM |
Mouse
(liquid, protein-A purified, PBS, BSA, azide) |
ELISA, IC, WB |
Rabbit, Chicken, Sea Urchin, Cow, Human, Mouse, Rat, Macaque |
immunogen = purified sea urchin --Lytechinus pictus-- sperm axonemal proteins (epitope localized in the C-terminal region of alpha and beta tubulins, the glutamylated motif at aa 445-457 of alpha tubulin) |
recognizes polyglutamylated Tubulin motif at amino acid 445-457 |
- |
|
|
Abcam |
monoclonal
IgG2b |
Mouse
(liquid, ascites fluid, azide) |
DB, ELISA, IH, RIA, WB |
Chicken, Sea Urchin, Cow, Pig, Human, Hamster, Mouse, Xenopus, Chlamydomonas reinhardtii |
immunogen = acetylated tubulin from the outer arm of Strongylo. purpuratus (sea urchin) |
recognizes Tubulin acetyl K40 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, afinity purified) |
IC, IF, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide derived from within residues 350 to the C-terminus of human neuron specific βIII tubulin, conjugated to KLH |
β III tubulin |
- |
|
|
Sigma Aldrich |
monoclonal
IgG2b |
Mouse
(liquid, purified Ig, PBS, azide) |
ELISA, IB, IC, IH |
Bovine, Human, Mouse, Rat |
immunogen = synthetic peptide corr. to the C-terminal sequence of human β-tubulin isotype III coupled to BSA |
recognizes isotype III of β-tubulin; does not bind tubulin in chicken brain preparation or chicken fribroblasts. |
- |
|
|
Sigma Aldrich |
monoclonal
IgG2b |
Mouse
(liquid, ascites, azide) |
ELISA, IB |
Bovine, Human, Rat |
immunogen = synthetic peptide corr. to the C-terminal sequence of human β-tubulin isotype III coupled to BSA |
specifically recognizes an epitope located on isotype III of β-tubulin; can stimulate microtubule assembly. No cross with other tubulin isotypes. |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG2a |
Mouse
(liquid, ascites, no preservative) |
IH, WB |
Mammal (various) |
immunogen = synthetic peptide (GEEEGE) |
α-tubulin, detyrosinated |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG |
Mouse
(liquid, purified, PBS, azide) |
IH, WB |
Rat |
- |
β III tubulin; clone is similar to the monoclonal antibody Tuj1 |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, ascites, PBS; BSA, azide) |
ELISA, IC, IH, WB |
Avian (various), Bovine, Sheep, Pig, Human, Monkey, Mouse, Rat |
immunogen = synthetic peptide corresp. to C-terminus aa 443-450 (E S E S Q G P K) of human class III β-tubulin conjugated to KLH |
reacts with the C-terminus of the neuron specific beta III isoform of tubulin, but not in glial cells |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, DEAE purified, PBS, BSA, azide) |
ELISA, IC, IH, IP, WB |
Bovine, Pig, Human, Monkey, Mouse, Rat |
immunogen = synthetic peptide corresponding to aa 443-450 (E S E S Q G P K) of human classIII beta-tubulin conj. to KLH. |
reacts with the C-terminus of the beta-III isoform of tubulin, known to be neuron specific; not beta tubulin in glial cells. |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(liquid, affinity purified, PBS, azide) |
WB |
Various species, Mouse |
immunogen = synthetic peptide from human beta III tubulin |
β III tubulin (~50kDa) |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
IgY |
Chicken
(liquid, affinity purified, PBS, azide) |
IC, IH |
Human, Mouse, Rat |
immunogen = synthetic peptide from human/rat beta III tubulin; Four different antibodies were combined to make this product |
β III tubulin; epitope = similar to TuJ1 antigen |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG3 |
Mouse
(liquid, ascites, PBS, no preservative) |
IH, WB |
Mammal (various) |
immunogen = synthetic peptide (E E E G E D E A) |
β II tubulin; a marker for radial glia populations, fibers and neural stem/ progenitor cells in developing brain |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG2a |
Mouse
(liquid, protein G purified, Tris-glycine saline, azide) |
IC, WB |
Bovine, Various species, Human, Mouse, Rat |
immunogen = peptide corresp. to aa 436-450 of neuronal specific β III tubulin; (C-M Y E D E E E S E A Q G P K) |
neuronal specific βIII tubulin; epitope maps to E A Q G P K |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgM |
Mouse
(ascites, azide) |
IH |
Bovine, Human, Rat |
immunogen = rat tubulin |
β Tubulin |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, protein G purified, PBS, BSA, tween-20, azide, conj. Alexa Fluor488 and FITC) |
FC, IC, IF, WB |
Bovine, Various species, Human, Mouse, Rat |
|
β-tubulin isotypes I, II, III, IVa, and IVb; epitope maps to aa 412-430 |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, protein G purified, PBS, BSA, tween-20, azide, conj. Alexa Fluor555 and FITC) |
FC, IC, IF, WB |
Bovine, Various species, Human, Mouse, Rat |
|
β-tubulin isotypes I, II, III, IVa, and IVb; epitope maps to aa 412-430 |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, protein G purified, PBS, azide) |
WB |
Bovine, Various species, Human, Mouse, Rat |
immunogen = purified bovine brain tubulin; epitope has been mapped to aa 412-430 |
β-tubulin isotypes I, II, III, IVa, and IVb; no cross-reactiviry with α- or γ-tubulin |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(affinity purified Ig, liquid, PBS) |
EM, IH, WB |
Mammal (various), Plant (various), Sea Urchin |
immunogen = peptide corr. to the C-terminal of delta 2 tubulin |
recognizes the delta 2 form of the tubulin alpha chain |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG2a |
Rat
(liquid, PBS, azide, purified) |
ELISA, IH, IP, RIA, WB |
Human |
immunogen = yeast tubulin; a linear seq. requiring an aromatic residue at the C-terminus, with the two adjacent aa being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin) |
alpha subunit of tubulin-specificity binding to so called Tyr-Tubulin |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(antisera, liquid, azide) |
EM, IB, IF, IH, WB |
Fish (various), Cat, Chicken, Gerbil, Guinea Pig |
immunogen = tubulin purified from chicken embryo brains |
specific for tubulin by counter IEP; may react to low and high molecular weight MAP’s |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG2b |
Mouse
(purified, liquid, PBS, azide) |
IC, IF, WB |
Various species |
immunogen = tubulin purified from polycephalum myxamoebae |
β tubulin; good for staining of microtubuli |
- |
|
|
AbD Serotec |
monoclonal
IgG2a |
Rat
(liquid, supernate, protein-G purified, azide) |
ELISA, IH, RIA, WB |
Mammal (various), Avian (various), Yeast |
immunogen = yeast tubulin |
recognises the alpha subunit of tubulin; reactivity pattern similar to clone YL1/2 (MCA77S) |
- |
|
|
Biolegend |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, PBS, azide, glycerol) |
IF, WB |
Human, Mouse |
immunogen = peptide conjugated to KLH |
recognizes 48 kDa γ-tubulin |
- |
|
|
Biolegend |
monoclonal
IgG1,κ |
Mouse
(liquid, affinity purified, PBS, azide) |
IH, WB |
Non-specific |
immunogen = fraction of tubulin purified from pig brain by two cycles of polymerization depolymerization |
recognizes α-tubulin |
- |
|
|
Biolegend |
monoclonal
IgM,κ |
Mouse
(liquid, affinity purified, PBS, azide, Alexa Fluor® 488) |
IF, WB |
Various species, Human, Mouse, Rat |
immunogen = recombinant full length human α-tubulin |
recognizes α-tubulin |
- |
|
|
Biolegend |
monoclonal
IgM,κ |
Mouse
(liquid, affinity purified, PBS, azide, Biotin conj.) |
IF, WB |
Various species, Human, Mouse, Rat |
immunogen = recombinant full length human α-tubulin |
recognizes α-tubulin |
- |
|
|
Biolegend |
monoclonal
IgM,κ |
Mouse
(liquid, affinity purified, PBS, azide) |
IF, WB |
Various species, Human, Mouse, Rat |
immunogen = recombinant full length human α-tubulin |
recognizes α-tubulin |
- |
|
|
Sigma Aldrich |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
IC, WB |
Mammal (various), Chicken, Ascidian, Nematode, Sea Urchin |
immunogen = Sarkosyl-resistant ribbons from sea urchin sperm axonemes |
reacts with beta tubulin; recognizes the two majorβ-tubulin isotypes and one of the minor β-tubulinisotypes |
- |
|
|
Sigma Aldrich |
monoclonal
IgG1 |
Mouse
(liquid, PBS, BSA, azide) |
ELISA, IC, IP, WB |
Bovine, Dog, Rabbit, Chicken, Sea Urchin, Human, Monkey, Hamster, Mouse, Rat |
immunogen = sea urchin sperm axonemal proteins (epitope in the C-terminal region of β-tubulin,aa 427-432 in sea urchin β2-tubulin) |
reacts with beta tubulin; all mouse brain β-tubulin isoforms (β and β' subpopulations), and sea urchin β2-tubulin |
- |
|
|
Sigma Aldrich |
monoclonal
IgG1 |
Mouse
(purified, PBS, BSA, azide, Cy3 conj.) |
IF |
Bovine, Rabbit, Frog, Wheat, Human, Hamster, Mouse, Rat, Moth |
immunogen = purified rat brain tubulin (epitope in the carboxy-terminal part of β-tubulin between aa 281-446) |
reacts with the chymotryptic β-Lc and β-Sc tubulin fragments and doesnot seem to exhibit mouse brain beta tubulin isoform related specificity |
- |
|
|
Sigma Aldrich |
monoclonal
IgG1 |
Mouse
(protein-A purified, gel filtered, PBS, BSA, azide, FITC conj.) |
IC, IH |
Bovine, Rabbit, Frog, Wheat, Human, Hamster, Mouse, Rat, Moth |
immunogen = purified rat brain tubulin (epitope in the carboxy-terminal part of β-tubulin between aa 281-446) |
reacts with the chymotryptic β-Lc and β-Sc tubulin fragments and doesnot seem to exhibit mouse brain beta tubulin isoform related specificity |
- |
|
|
Sigma Aldrich |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
IF, WB |
Bovine, Rabbit, Chicken, Frog, Wheat, Human, Hamster, Mouse, Rat, Moth |
immunogen = purified rat brain tubulin |
reacts with β-Lc and β-Sc fragments in the carboxy-terminal part of beta tubulin in immunoblotting. The antibody may be used to localize β-tubulin in cultured cells or tissue sections |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(IgG, PBS, gelatin, azide) |
IF, IH, IP, WB |
Zebra fish, Human, Mouse, Rat, Xenopus, C. elegans, Drosophila |
recomb. protein corresp. to aa 210-444 mapping at the C-terminus of human β-Tubulin |
beta Tubulin |
- |
|
|
Novus Biologicals |
monoclonal
IgG1,κ |
Mouse
(liquid, PBS, azide) |
EM, IF, IH, WB |
Chicken, Cow, Pig, Human, Gerbil, Guinea Pig, Mouse, Rat, Xenopus |
immunogen = native chicken brain microtubules; epitope = aa 426-450 |
57 kDa protein identified as α-tubulin |
- |
|
|
Novus Biologicals |
monoclonal
IgG1 |
Mouse
(liquid, PBS, BSA, azide) |
IF |
Amphibian (various), Goat, Chicken, Sea Urchin, Nicotiana (tobacco), Fungi, Yeast, Cow, Human, Monkey, Gerbil, Hamster, Mouse, Rat, Trypanosome, Kangaroo rat |
immunogen = purified chick brain microtubules |
α-tubulin (C-terminal) |
- |
|
|
Novus Biologicals |
monoclonal
IgM |
Mouse
(liquid, PBS, azide) |
IC, IP, WB |
Plant (various), Chicken, Protozoa, Cow, Pig, Human, Hamster, Mouse, Rat |
immunogen = full length purified native protein from pig α-tubulin |
α-tubulin (denaturing and non-denaturing conditions) |
- |
|
|
Novus Biologicals |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, purified) |
IC |
- |
immunogen = synthetic peptide: corresp. to aa (C)441-448 (E S E S Q G P K) of β III Tubulin conj. to maleimide activated KLH via cysteine added to the N- terminus of the neurone specific peptide |
recognizes the peptide sequence E S E S Q G P K (aa 441-448) of human class III beta tubulin specific for neurones |
- |
|
|
Novus Biologicals |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide) |
IH, IP, WB |
Chicken, Carp, Various animal, Cow, Pig, Baboon, Human, Hamster, Mouse, Rat |
immunogen = synthetic peptide: corresp. to aa (C)441-448 (E S E S Q G P K) of human β III Tubulin |
class III beta tubulin specific for neurones |
- |
|
|
Novus Biologicals |
polyclonal
|
Chicken
(liquid, PBS, PBS, azide, affinity purified) |
IC, IH |
Mouse |
immunogen = four different affinity-purified anti-peptide antibodies were combined to make this product |
βIII Tubulin |
- |
|
|
Novus Biologicals |
monoclonal
IgG2b |
Mouse
(liquid, raw ascites, azide) |
ELISA, WB |
Cow, Human, Rat |
immunogen = synthesized peptide corr. to the C-term. seq. of human β-tubulin isotype III, coupled to BSA |
specifically recognizes an epitope located on human β-tubulin, isotype III |
- |
|
|
Novus Biologicals |
monoclonal
IgM |
Mouse
(liquid, PBS, azide, purified) |
IC, WB |
- |
immunogen = full length native protein (purified) of brain microtubule protein MTP-1 (Pig) |
reacts with an epitope located within aa 345-430 of C-terminal domain of β-tubulin subunit |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, sera, PBS, azide, purified) |
WB |
Human |
immunogen = synthetic peptide made to the N-terminal region of human βtubulin (within residues 1-100); Swiss-Prot P07437 |
βTubulin |
- |
|
|
Novus Biologicals |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
ELISA, IH, WB |
Dog, Chicken, Cow, Human, Hamster, Mouse, Rat, Xenopus |
immunogen = synthetic peptide corresp. to N-terminal aa 38-53 of γ-tubulin, conj. to KLH |
γ-tubulin; location centrosomal |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, purified, PBS, azide) |
IF, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide: D E Y H A A T R P D Y I S W G T Q E Q-K, with N-terminally added lysine corresp. to aa 433-451 of γ-Tubulin, conj. to KLH, |
centrosome marker; recognizes 48 kDa γ-tubulin; does not react with α, β, ω or η tubulin |
- |
|
|
Novus Biologicals |
monoclonal
IgG2a |
Mouse
(liquid, protein-A purified, PBS, BSA, azide) |
ELISA, WB |
Chicken, Frog, Human, Mouse |
immunogen = synthetic peptide conj. to KLH, corr. to aa 352-366 of human ε-Tubulin |
recognizes 55 kDa ε-tubulin |
- |
|
|
Novus Biologicals |
monoclonal
IgG2a |
Rat
(liquid, glycerol, azide) |
ELISA, IC, IF, IH, IP, RID, WB |
Mammal (various), Allium, Slime molds, S. cerevisiae, S. pombe |
immunogen = full length native protein (purified) (S. cerevisiae) |
detects the tyrosinated form of the alpha-tubulin subunit |
- |
|
|
Novus Biologicals |
monoclonal
IgG2a |
Rat
(liquid, glycerol, azide) |
ELISA, IF, IH, RID, WB |
Mammal (various), Allium, Slime molds, S. cerevisiae, S. pombe |
immunogen = full length native protein (purified) (S. cerevisiae |
detects an epitope on α-tubulin between the aa 414-422 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(affinity purified, PBS, gelatin, azide; also FITC, AC or HRP conj.) |
IF, IH, IP, WB |
Human, Mouse, Rat, Xenopus |
carboxy terminus of gamma tubulin of human origin |
gamma tubulin only; no cross with other tubulins |
- |
|
|
Sigma Aldrich |
polyclonal
IgG |
Rabbit
(liquid, affinity isolated, PBS, BSA, azide) |
IF, IP, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide corr. to aa residues 441-450 of human β-tubulin III with N-terminal added cysteine, conjugated to KLH |
~55 kDa β-Tubulin III, in WB detectable in human white blood cells or platelets |
- |
|
|
Seikagaku |
monoclonal
|
Mouse
(liquid, ascites fluid, azide) |
IB, IF, IP |
Amphibian (various), Bovine, Chicken, Fungi, Yeast, Human, Mouse, Rat |
immunogen = purified chick brain microtubules |
specific for alpha tubulin |
- |
|
|
Upstate and Chemicon are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, protein G purified, PBS, BSA, tween-20, azide; conj. FITC and Alexa Fluor® 555) |
IF |
Avian (various), Bovine, Pig, Human, Guinea Pig, Mouse, Rat |
immunogen = purified human brain α-Tubulin |
α-Tubulin |
- |
|
|
Upstate and Chemicon are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, protein G purified, PBS, BSA, tween-20, azide, conj. Alexa Fluor488 and FITC) |
FC, IC, IF, WB |
Avian (various), Bovine, Pig, Human, Guinea Pig, Rat |
immunogen = purified human brain α-Tubulin |
reacts with the aa seq. 426-450 on alpha-tubulin (A A L E K D Y E E V G V D S V E G E G E E E G E E) |
- |
|
|
Upstate and Chemicon are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, protein G purified, PBS, glycerol, azide) |
FC, IC, IF, WB |
Avian (various), Bovine, Pig, Human, Guinea Pig, Mouse, Rat |
immunogen = purified human brain α-Tubulin |
α-tubulin; epitope recognized by this antibody has been mapped to amino acids 426-450 |
- |
|
|
Sigma Aldrich |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, purified) |
IB, IEP, IF, IH, WB |
Plant (various), Bovine, Rabbit, Chicken, Frog, Sea Urchin, Wheat, Human, Hamster, Mouse, Rat, Moth |
immunogen = tubulin from rat brain (epitope in the carboxy-terminal part of β-tubulin between aa 281-446) |
reacts with beta tubulin; recognizes all five isoforms of β-tubulin (β1-β5); reacts with the β-Lc and β-Sc fragments in the C-terminal part of β−tubulin in immunoblotting |
- |
|
|
Sigma Aldrich |
monoclonal
IgG3 |
Mouse
(liquid, ascites, azide) |
IC, WB |
Bovine, Dog, Marsupial, Yeast, Monkey, Mouse, Xenopus |
immunogen = peptide containing the carboxy terminal amino acids of α-tubulin |
tyrosinated tubulin |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(affinity purified Ig, liquid, PBS) |
IH, WB |
Mammal (various), Plant (various), Sea Urchin |
immunogen = peptide corr. to the C-terminal of Glu tubulin |
recognizes the detyrosinated form (Glutubulin) of the tubulin alpha chain |
- |