|
|
Sigma Aldrich |
polyclonal
IgG |
Rabbit
(whole antiserum, delipidized, azide) |
IC, WB |
- |
immunogen = tubulin purified from 13 day old chicken embryos |
specific for tubulin and may contain antibodies to high and low molecular weight MAP's |
- |
|
|
Sigma Aldrich |
polyclonal
IgG |
Rabbit
(liquid, affinity isolated, PBS, BSA, azide) |
IC, WB |
Human, Chicken |
immunogen = synthetic peptide corr. to theN-terminal region of human γ-tubulin (aa 38-53, with C-terminally added lysine) |
specific for γ-tubulin; epitope not found in α-, β-, δ- and ε-tubulins |
- |
|
|
Sigma Aldrich |
polyclonal
IgG |
Rabbit
(liquid, IgG fraction, PBS, azide) |
IC, WB |
Human, Mouse, Xenopus |
immunogen = synthetic peptide corr. to the C-terminus of human γ-tubulin (aa 433-451, with N-terminally added lysine) conjugated to KLH |
specific for γ-tubulin and not found in other members of the tubulin family |
- |
|
|
Sigma Aldrich |
polyclonal
IgG |
Rabbit
(liquid, IgG fraction, PBS, azide) |
IC, WB |
Human, Rat, Xenopus |
immunogen = synthetic peptide corr. to the C-terminus of xenopus γ-tubulin (aa 437-451, with N-terminally added cysteine- glycine) conjugated to KLH |
specific for γ-tubulin and not found in other members of the tubulin family |
- |
|
|
Sigma Aldrich |
monoclonal
IgM |
Mouse
(liquid, PBS, azide) |
IC, WB, ELISA |
Human |
immunogen = human recomb. δ-tubulin |
~55 kDa δ-tubulin |
- |
|
|
Sigma Aldrich |
monoclonal
IgG2a |
Mouse
(liquid, PBS, BSA, azide) |
WB, ELISA |
Human, Mouse, Chicken, Frog |
immunogen = synthetic peptide (corr. to aa 352-366) in the C-terminal region of human ε-tubulin, conjugated to KLH |
C-terminal region of ε-tubulin |
- |
|
|
BD Pharmingen |
monoclonal
IgM |
Mouse
(aqueous, buffered, azide; affinity purified from ascites or supernatant) |
IH, WB |
Bovine, Human, Mouse, Rat |
|
β-Tubulin; no cross with α–tubulin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum, treated to remove lipoproteins, azide) |
IF, WB |
Chicken |
immunogen = protein purified from 13 day old chicken embryos |
tubulin; has not been adsorbed on MAPs and therefore may contain antibodies to high and low molecular weight MAPs |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, protein-A purified, PBS, BSA, azide, FITC conj.) |
IF |
Amphibian (various), Goat, Chicken, Sea Urchin, Fungi, Cow, Human, Monkey, Gerbil, Hamster, Mouse, Rat |
immunogen = Purified chick brain microtubules (epitope in the carboxy-terminal part of α-tubulin) |
α-Tubulin |
- |
|
|
Abcam |
monoclonal
IgG2b |
Mouse
(liquid, raw ascites, azide) |
ELISA, WB |
Cow, Human, Rat |
immunogen = synthesized peptide corr. to the C-term. sequence of human β-tubulin isotype III, coupled to BSA |
specifically recognizes an epitope located on human β-tubulin, isotype III |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, raw ascites, azide) |
IF, WB |
Cow, Human, Mouse, Rat |
immunogen = purified rat brain tubulin |
55 kDa β-tubulin |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, Cy3 conj, gel filtered, BSA, PBS, azide) |
IC, IF, IH |
Rabbit, Frog, Wheat, Cow, Human, Hamster, Mouse, Rat, Moth |
immunogen = purified rat brain tubulin (epitope in the C-terminal part of β-tubulin, between aa 281-446) |
reacts with the chymotryptic beta-Lc and beta-Sc tubulin fragments and does not seem to exhibit mouse brain β-tubulin isoform-related specificity |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, protein-A purified, FITC conj, gel filtered, BSA, PBS, azide) |
IC, IF, IH |
Rabbit, Frog, Wheat, Cow, Human, Hamster, Mouse, Rat, Moth |
immunogen = purified rat brain tubulin (epitope in the C-terminal part of β-tubulin, between aa 281-446) |
reacts with the chymotryptic beta-Lc and beta-Sc tubulin fragments and does not seem to exhibit mouse brain β-tubulin isoform-related specificity |
- |
|
|
Abcam |
monoclonal
IgG2a |
Mouse
(liquid, protein-A purified, PBS, BSA, azide) |
ELISA, WB |
Chicken, Frog, Human, Mouse |
immunogen = synthetic peptide conj. to KLH, corr. to aa 352-366 of human ε-Tubulin |
recognizes 55 kDa ε-tubulin |
- |
|
|
Abcam |
monoclonal
IgG2a |
Rat
(liquid, IgG fraction, PBS, glycerol, azide) |
ELISA, IF, IH, RIA, WB |
Mammal (various), Allium, Slime molds, S. cerevisiae, S. pombe |
immunogen = full length S. cerevisiae native protein, purified (epitope between aa 414-422) |
has a higher affinity for fixed microtubules (formaldehyde or glutaraldehyde) than native microtubules when performing IHC |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, ascites fluid, azide) |
ELISA, IH, WB |
Chicken, Cow, Human, Hamster, Mouse, Rat |
immunogen = synthetic peptide corr. to the C-terminal sequence, coupled to BSA |
recognizes 55 kDa β-tubulin, isotype IV; does not react with other tubulin isotypes |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, raw ascites, azide) |
IC, IF, WB |
Mammal (various), Chicken, Sea Urchin |
immunogen = Sarkosyl-resistant ribbons from sea urchin sperm axonemes |
recognizes the two major beta tubulin isotypes |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, supernate, BSA, PBS, azide, predilute) |
IH |
Human |
immunogen = synthetic peptide: ESESQGPK, human |
βIII-tubulin |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, ascites, purified, PBS, azide) |
ELISA, IC, IF, IP, WB |
Mammal (various), Various species |
immunogen = fraction of tubulin purified from pig brain by two cycles of polymerization- depolymerization (epitope at aa 65-97 on N-terminal structural domain) |
~55 kDa alpha tubulin |
- |
|
|
Abcam |
monoclonal
IgM |
Mouse
(liquid, ascites, purified, PBS, azide) |
IC, IP, WB |
Plant (various), Chicken, Protozoa, Cow, Pig, Human, Hamster, Mouse, Rat |
immunogen = full length native pig protein, purified |
binds tubulin under denaturing and non-denaturing conditions |
- |
|
|
Abcam |
monoclonal
IgM |
Mouse
(liquid, ascites, purified, PBS, azide) |
ELISA, IC, IP, WB |
Plant (various), Chicken, Carp, Protozoa, Cow, Pig, Human, Hamster, Mouse, Rat, S. cerevisiae, S. pombe |
immunogen = Beta-subunits of pig tubulin, purified |
N-terminal structural domain of β-tubulin of wide variety of species; all charge variants of tubulin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide, affinity purified) |
WB |
Human |
immunogen = synthetic peptide mapping near the N-terminus of human beta-tubulin |
β-Tubulin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IC, IF, WB |
Chicken, Human, Mouse, Rat, Chinese hamster |
immunogen = synthetic peptide derived from within residues 1-100 of human beta Tubulin, conj. to KLH |
β-Tubulin (50kDa) |
- |
|
|
Abcam |
polyclonal
IgG |
Goat
|
IC, IF, WB |
Human |
immunogen = synthetic peptide derived from within residues 1-100 of human beta-Tubulin |
β-Tubulin (~50kDa) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, glycerol, BSA, no preservative; conj. to HRP) |
WB |
Human |
immunogen = = synthetic peptide derived from within residues 1-100 of human beta Tubulin, conjugated to KLH |
β-Tubulin (50kDa) |
- |
|
|
Abcam |
monoclonal
IgG2b |
Mouse
(liquid, raw ascites, azide) |
IF, WB |
Chicken, Cow, Pig, Human, Mouse, Rat |
immunogen = chemically synthesized peptide corr. to the carboxyl-terminal sequence of human β-tubulin isotype II coupled to BSA |
recognizes isotypes I and II of β-Tubulin |
- |
|
|
Abcam |
monoclonal
IgM |
Mouse
(liquid, PBS, azide, purified) |
IC, WB |
- |
immunogen = microtubule proteins from porcine brain |
recognizes recognizes α, β-tubulin heterodimer, a basic intracellularstructural unit of microtubules |
- |
|
|
Abcam |
monoclonal
IgG1,κ |
Mouse
(liquid, PBS, glycerol, no preservative) |
ELISA, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to C- terminal aa of human α-tubulin, conjugated to KLH |
α-tubulin |
- |
|
|
Abcam |
monoclonal
IgG2a |
Mouse
(liquid, protein A purified) |
IF, WB |
- |
immunogen = rat brain tubulin |
α-tubulin |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide) |
IC, IF |
Pig, Human, Mouse, Rat |
immunogen = fraction of tubulin purified from pig brain by two cycles of polymerization-depolymerization |
recognizes the defined epitope (aa 65-97) on N-terminal structural domain of α-tubulin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IC, IF, WB |
Chicken, Cow, Human, Mouse, Rat |
immunogen =synthetic peptide derived from within residues 1-100 of human α- Tubulin, conjugated to KLH |
α-Tubulin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
|
IC, IF, WB |
Chicken, Human, Mouse, Chinese hamster, Drosophila |
immunogen = synthetic peptide derived from within residues 400 to the C-terminus of human α- tubulin, conjugated to KLH |
α- tubulin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IH, IP, WB |
Cow, Pig, Human, Gerbil, Guinea Pig, Mouse, Rat, Xenopus |
immunogen = synthetic peptide, A L E K D Y E E V G V D S V E G E G E E E G E E Y, corresp. to C- terminal aa 426-450 of human α- Tubulin |
α- Tubulin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, no preservative) |
WB |
Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to C-terminalresidues of human α-tubulin, conjugated to KLH, |
α-tubulin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IH |
Chicken, Cow, Pig, Human, Gerbil, Guinea Pig, Mouse, Rat, Xenopus |
immunogen = synthetic peptide A A L E K D Y E E V G V D S V E G E G E E E G E E , corresp. to aa 426-450 of human α-Tubulin |
α-Tubulin |
- |
|
|
Abcam |
monoclonal
IgG2b |
Mouse
(liquid, ascites, no preservative) |
IC, WB |
Sea Urchin, Human, Mouse, Rat |
immunogen =tissue / cell preparation - acetylated α-tubulin from the axoneme ofsea urchin sperm flagella |
Acetylated α-Tubulin |
- |
|
|
Abcam |
monoclonal
IgG1,κ |
Mouse
(liquid, PBS, azide, protein G purified) |
EM, IF, IH, IP, WB |
Dog, Chicken, Cow, Pig, Human, Gerbil, Guinea Pig, Mouse, Rat, Xenopus |
immunogen = purified full length native protein of chicken α-Tubulin (extracted from brain). |
α-Tubulin |
- |
|
|
Abcam |
polyclonal
IgY |
Chicken
(;;iquid, PBS, azide, affinity purified) |
IC, IF, IH, WB |
Human, Rat |
immunogen = 4 different synthetic peptides corresp. to regions of the βIII tubulin, conjugated to KLH |
βIII-Tubulin |
- |
|
|
Abcam |
monoclonal
IgG2a |
Mouse
(liquid, PBS,, trehalose, no preservative, protein G purified) |
IC, IF, IH, WB |
Human, Mouse, Rat |
immunogen = tissue/cell preparation (microtubules derived from rat brain) |
beta III Tubulin - Neuronal Marker |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, glycerol, thimerosal, no preservative, affinity purified) |
WB |
Mammal (various) |
immunogen = commercially sensitive |
neuron specific class III ß-tubulin (ßIII); not ß-tubulin found in glial cells |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, raw ascites, azide) |
ELISA, IC, IH, WB |
Dog, Chicken, Cow, Human, Hamster, Mouse, Rat, Xenopus |
immunogen = synthetic peptide corr. to the C-terminal sequence of β-tubulin isotype I, coupled to BSA |
recognizes βI-Tubulin; no reactivity with other tubulin isotypes is observed |
- |
|
|
Abcam |
monoclonal
IgM |
Mouse
(liquid, ascites, azide) |
IF, IH, WB |
- |
immunogen = tissue preparation from rat brain |
β-tubulin |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, protein-A purified, PBS, BSA, azide) |
ELISA, IC, IP, WB |
Dog, Rabbit, Chicken, Sea Urchin, Dinoflagellate, Cow, Human, Hamster, Mouse, Rat, Chlamydomonas reinhardtii |
immunogen = sea urchin (Lytechinus pictus) sperm axonemal proteins (aa 427-432 in sea urchin β-2 tubulin) |
all mouse brain β-tubulin isoforms (beta and beta subpopulations), and sea urchin β2-tubulin |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, azide) |
EM, IF, IH, IP, WB |
Chicken, Cow, Pig, Human, Gerbil, Guinea Pig, Mouse, Rat, Xenopus |
immunogen = native chicken brain microtubules; epitope = aa 426-450 |
β-Tubulin |
- |
|
|
Abcam |
monoclonal
IgG1,κ |
Mouse
(liquid, PBS, azide, protein G purified) |
IH, IP, WB |
Chicken |
immunogen = native chick brain microtubules; epitope maps to aa 416-430 of β- Tubulin |
β-tubulin (54kDa); no cross with α-tubulin |
- |
|
|
Abcam |
monoclonal
IgM |
Mouse
(liquid, PBS, azide, purified) |
IC, WB |
- |
immunogen = purified full length native protein of pig brain microtubule protein MTP-1 |
β-Tubulin; epitope maps to aa 345-430 of C-terminal |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IH, IP, WB |
Chicken, Sea Urchin, Fungi, Cow, Pig, Human, Gerbil, Guinea Pig, Mouse, Rat, S. cerevisiae |
immunogen = synthetic peptide: N D L V S E Y Q Q Y Q D A T A, corresp. to aa 416-430 of human β-Tubulin |
β-Tubulin |
- |
|
|
Abcam |
polyclonal
IgY |
Chicken
(liquid, PBS, affinity purified) |
WB |
Cow |
immunogen =full length β- tubulin (cow) |
β- tubulin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IH |
Chicken, Sea Urchin, Fungi, Cow, Human, Gerbil, Guinea Pig, Mouse, Rat, S. cerevisiae |
immunogen = synthetic peptide: N D L V S E Y Q Q Y Q D A T A, corresp. to aa 416-430 of human β-Tubulin |
β-Tubulin |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, ascites fluid, azide) |
ELISA, IH, WB |
Dog, Chicken, Cow, Human, Hamster, Mouse, Rat, Xenopus |
immunogen = synthetic peptide conjugated to KLH, corr. to the N-terminal aa 38-53 of γ-Tubulin |
recognizes 48 kDa γ-tubulin |
- |
|
|
Abcam |
monoclonal
IgG2b |
Mouse
(liquid, PBS, azide, protein A purified) |
IC, WB |
Plant (various), Protozoa, Pig, Human, Mouse, Rat |
immunogen =synthetic peptide: E Y H A A T R P D Y I S W G T Q conj to KLH, corresp. to aa 434-449 of γ-tubulin |
γ-tubulin; epitope located in the C-terminal region |
- |
|
|
Abcam |
monoclonal
IgG2b |
Mouse
(liquid, PBS, azide, purified; cpnj. Dyomics® 547) |
IC |
Plant (various), Chicken, Protozoa, Pig, Human, Mouse, Rat, Xenopus |
immunogen = synthetic peptide E Y H A A T R P D Y I S W G T Q conj. to KLH, corresp. to aa 434/449 of γ- tubulin |
γ- tubulin; epitope maps at C-terminal |
- |
|
|
Abcam |
monoclonal
IgG2b |
Mouse
(liquid, PBS, azide, protein A purified; conj. Dyomics® 647) |
IC |
Plant (various), Chicken, Protozoa, Pig, Human, Mouse, Rat, Xenopus |
immunogen = synthetic peptide: E Y H A A T R P D Y I S W G T Q conjugated to KLH, corresp. to aa 434/449 of human γ-tubulin |
γ-tubulin; epitope maps in the C-terminal region |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
IF, WB |
Human |
immunogen = recombinant fragment human |
γ-Tubulin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IC, IF, WB |
Dog, Zebra fish, Human, Mouse, Rat, Xenopus |
immunogen = synthetic peptide derived from within residues 1 - 100 of human γ-Tubulin, conjugated to KLH |
γ-Tubulin (seq. found in 1 and 2); no cross-reaction with α- and β- tubulin |
- |
|
|
Abcam |
monoclonal
IgG2a |
Rat
(liquid, PBS, glycerol, azide) |
ELISA, IC, IF, IP, RIA, WB |
Allium, Slime molds, S. cerevisiae, S. pombe |
immunogen = full length native protein (purified) (S. cerevisiae) |
tubulin; detects the tyrosinated form of the α-tubulin subunit; recognizes a short aa sequence Glu-Glu-Phe(OH) which can be inserted into the C-terminal domain of fusion proteins |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, IgG fraction, azide) |
IC, IF, WB |
Human |
immunogen = synthetic peptide conjugated to KLH (epitope: E E F A T E G T D R K D V F F Y K, aa 38-53) |
recognizes 48 kDa γ-tubulin; does not react with alpha or beta tubulin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, gel filtration, BSA, PBS, azide, Cy3 conj) |
IF |
Chicken, Human |
immunogen = synthetic peptide conjugated to KLH (epitope: E E F A T E G T D R K D V F F Y, aa 38-53) |
recognizes 48 kDa γ-tubulin; does not react with alpha, beta, omega or eta tubulin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, IgG fraction, PBS, azide) |
IF, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide: D E Y H A A T R P D Y I S W G T Q E Q K, with N-terminally added Lysine, conjugated to KLH, corr. to aa 433-451 of γ-Tubulin |
recognizes 48 kDa γ-tubulin; does not react with alpha, beta, omega or eta tubulin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, antiserum, IgG fraction, purified, PBS, azide) |
IF, WB |
Fish (various), Zebra, Human, Mouse, Rat, Xenopus |
immunogen = synthetic peptide: A A T R P D Y I S W G T Q E Q C G, with N-terminally added cysteine-glycine, conj. to KLH, corr. to aa 437-451 of Xenopus laevis γ-Tubulin |
recognizes 48 kDa γ-tubulin; does not react with alpha, beta, omega or eta tubulin |
- |
|
|
Covance Research Products, Inc (now BioLegend) |
monoclonal
IgG2a,κ |
Mouse
(purified) |
WB |
Mammal (various) |
immunogen = peptide sequence C G E E E G E |
55 kDa detyrosinated α-tubulin |
- |
|
|
AbD Serotec |
monoclonal
IgM |
Mouse
(lyophilized, PBS, no preservative) |
IF, IH, WB |
Bovine, Human, Hamster, Mouse, Rat |
immunogen = native brain tubulin |
WB specific for tubulin. IF it reacts with alpha and beta microtubules, but not with microfilaments or intermediate filaments |
- |
|
|
AbD Serotec |
polyclonal
|
Rabbit
(liquid, PBS, azide, protein A purified) |
ELISA, WB |
Pig |
immunogen = purified porcine brain tubulin |
tubulin |
- |
|
|
Sigma Aldrich |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
ELISA, IC, IH, WB |
Bovine, Dog, Chicken, Human, Hamster, Mouse, Rat, Xenopus |
immunogen = synthetic peptide corr. to the C-terminal sequence of β-tubulin isotype I coupled to BSA |
specifically recognizes an epitope located in the C-terminal sequence of β-tubulin isotype I |
- |
|
|
Sigma Aldrich |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
ELISA, IH, WB |
Bovine, Chicken, Human, Hamster, Mouse, Rat |
immunogen = synthetic peptide corr. to the C-terminal sequence of β-tubulin isotype IV coupled to BSA |
specifically recognizes an epitope located in the C-terminal sequence of β-tubulin isotype IV; no reactivity with other tubulin isotypes is observed |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, conj. to FITC) |
IC |
Human |
immunogen = synthetic peptide: corresp.to aa 441-448 (ESESQGPK) of β III tubulin conj. to maleimide activated KLH via cysteine added to the N-terminus of the neurone specific peptide |
class III β-tubulin specific for neurones |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, ascites, purified, PBS, azide) |
IC, IH, IP, WB |
Mammal (various), Chicken, Carp, Cow, Pig, Baboon, Human, Hamster, Mouse, Rat |
immunogen = synthetic peptide: ESESQGPK conj. to malemide activated KLH (aa 441-448 of human tubulin) |
binds to class III beta-tubulin, specific for neurones |
- |
|
|
Abcam |
monoclonal
IgG3 |
Mouse
(liquid, PBS, thimerosal, protein A purified) |
IF, WB |
Mammal (various) |
immunogen = synthetic peptide, corresp. to aa CEEEEGEDEA at the C-terminus of human βII Tubulin |
βII Tubulin |
- |
|
|
Abcam |
monoclonal
IgG3 |
Mouse
(liquid, protein A purified) |
IF, WB |
Mammal (various) |
immunogen = synthetic peptide, CEEEEGEDEA, found at the C-terminus of βII tubulin |
βII tubulin |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Rat
(protein G purified) |
ELISA, IH, IP, RIA, WB |
Mammal (various), Avian (various), Yeast, Human |
immunogen = yeast tubulin |
α-Tubulin |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Rat
(protein G purified) |
ELISA, IH, RIA, WB |
Mammal (various), Avian (various), Yeast, Human |
immunogen = yeast tubulin |
α-Tubulin |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(DEAE-column chromatography) |
ELISA, IF, WB |
Various species, Human |
immunogen = synthetic peptide corresp. to the position α- of tubulin (65-79) |
α-Tubulin (50kD) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(Protein A purified) |
IF, IP, WB |
Human, Mouse, Rat |
immunogen = sarkosyl-resistant filament from sea urchin sperm axonemes |
α-Tubulin (50.5kD), all forms |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(affinity purified) |
ELISA, IH |
Bovine, Porcine, Chicken, Frog, Human, Gerbil, Guinea Pig, Mouse, Rat |
immunogen = synthetic peptide derived from aa426-450 of human α- tubulin |
α-Tubulin |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(affinity purified) |
ELISA, IF, IH, WB |
Human |
immunogen = synthetic peptide corresp. to aa427-441 of human α- tubulin |
α-Tubulin |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(protein G purified) |
IC, WB |
Avian (various), Bovine, Porcine, Human, Guinea Pig, Mouse, Rat |
immunogen = purified human brain α-Tubulin mapping to aa426-450 (A A L E K D Y E E V G V D S V E G E G E E E G E E) |
α-Tubulin (50-60kD) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(protein A purified) |
IF, WB |
Human |
immunogen = acetylated α-tubulin from the axoneme of sea urchin sperm flagella |
α-Tubulin (~55kD) acetylated form |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(protein G purified) |
IH, WB |
Various species, Human |
immunogen = β-Tubulin |
β-Tubulin |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(unpurified serum) |
WB |
C. elegans |
immunogen = recombinant protein (domain residues 160-421) |
α-Tubulin |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Chicken
(affinity purified) |
WB |
Bovine |
immunogen = synthetic peptide of β-Tubulin |
β-Tubulin |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(Ion exchange chromatography) |
ELISA, IC, IH, IP, WB |
Bovine, Porcine, Human, Monkey, Mouse, Rat |
immunogen = synthetic peptide corresp. to aa 443-450 (E S E S Q G P K) of human class III β-Tubulin coupled to KLH |
recognizes the C-terminus of the β-III isoform of tubulin; not in glial cells |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(ascites) |
ELISA, IC, IH, IP, WB |
Human, Mouse |
immunogen = mouse Tubulin β-III peptide C-E S E S Q G P K |
β-III isoform of tubulin |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(ascites) |
ELISA, IC, IH, WB |
Human |
immunogen = synthetic peptide corresp. to aa 441-448 of human III-β-tubulin |
β-III isoform of tubulin |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(DEAE-column chromatography) |
IH, WB |
Bovine, Porcine, Baboon, Human, Mouse, Rat, Syrian hamster |
immunogen = synthetic peptide corresp. to an epitope within the C-terminal region of human class III-β-tubulin |
β-III isoform of tubulin |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(IgG fractionation) |
IH, WB |
Pig, Human, Hamster, Mouse, Rat |
immunogen = synthetic peptide made to the N-terminal region of human β-Tubulin (within residues 1-100). Swiss-Prot P07437 |
β-Tubulin |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Rabbit
(DEAE-column chromatography) |
ELISA, IH, IP, WB |
Bovine, Porcine, Chicken, Frog, Sea Urchin, Fungi, Yeast, Human, Gerbil, Guinea Pig, Mouse, Rat, Myxamoeba, Plasmodium |
immunogen = synthetic peptide derived from aa416-430 of human β-Tubulin |
β-Tubulin |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
|
Chicken
(affinity purified) |
WB |
Human, Mouse, Rat |
immunogen = synthetic peptide |
β-Tubulin |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, protein-A purified, PBS, azide) |
IC, IF, IP, RIA, WB |
Chicken, Sea Urchin, Cow, Human, Mouse, Rat, Chlamydomonas reinhardtii, African green monkey, Kangaroo rat |
immunogen = sarkosyl-resistant filaments from sea urchin (epitope at C-terminal end of the α-tubulin isoform |
~50 kDa α-Tubulin |
- |
|
|
Biogenesis AdB Serotec |
monoclonal
IgM |
Mouse
(lyophilized, PBS, ascites) |
IF, IH, WB |
Bovine, Human, Hamster, Mouse, Rat |
immunogen = native, from brain |
IF recognizes alpha and beta microtubules but not with microfilaments or intermediate filaments |
- |
|
|
Biogenesis AdB Serotec |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, protein A purified) |
ELISA, WB |
Pig |
immunogen = native, from brain |
tubulin |
- |
|
|
Biogenesis AdB Serotec |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide) |
ELISA, WB |
Pig, Human, Mouse, Rat |
immunogen = purified porcine brain tubulin |
tubulin |
- |
|
|
Biogenesis AdB Serotec |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, protein A purified) |
ELISA, WB |
Pig |
immunogen = native, from brain |
tubulin |
- |
|
|
Biotrend |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, protein A purifed) |
ELISA, WB |
Pig |
immunogen = native, from brain |
tubulin |
- |
|
|
Biotrend |
monoclonal
IgM |
Mouse
(lyophilized, PBS, ascites) |
IF, IH, WB |
Bovine, Various species, Human, Hamster, Mouse, Rat |
|
IF reacts with alpha and beta microtubules but not with microfilaments or intermediate filaments |
- |
|
|
Covance Research Products, Inc (now BioLegend) |
monoclonal
IgG2a,κ |
Mouse
(ascites) |
WB |
Mammal (various) |
immunogen = peptide sequence C G E E E G E |
55 kDa detyrosinated α-tubulin |
- |
|
|
MP Biomedicals |
monoclonal
IgG1 |
Mouse
(liquid, ascites) |
IB, IF |
Bovine, Chicken, Human, Mouse |
immunogen = purified rat brain tubulin |
β-tubulin |
- |
|
|
MP Biomedicals |
monoclonal
IgG1 |
Mouse
(liquid, ascites, PBS, BSA) |
IH, WB |
Amphibian (various), Bovine, Chicken, Fungi, Yeast, Human, Mouse |
immunogen = alpha-tubulin isolated from chick brain microtubules |
α-Tubulin |
- |
|
|
MP Biomedicals |
monoclonal
IgG1, κ |
Mouse
(liquid, ascites, PBS, BSA; preservative depends on lot number) |
IH, WB |
Chicken, Human |
immunogen = β-tubulin isolated from chick brain microtubules |
β-Tubulin in fibroblasts, microtubules and mitotic spindles |
- |
|
|
MP Biomedicals |
polyclonal
|
Rabbit
(antiserum, azide) |
IEP, IF, WB |
Chicken |
immunogen = purified tubulin (α & β chains) from chick embryo brain; serum may contain antibodies to high and low molecular weight MAP's in addition to the antibodies to tubulin |
Tubulin and microtubule |
- |