|
|
Zymed/ Invitrogen |
polyclonal
Ig |
Rabbit
(antiserum, PBS, azide, affinity purified) |
IF, IH, WB |
Dog, Human, Mouse, Rat |
immunogen = synthetic peptide derived from the mid region of human ZO-1, which differs from dog, mouse, and rat by one conservative amino acid replacement |
reacts with the ~225 kDa ZO-1 protein; bands of unknown origin may be observed at lower molecular weights. |
- |
|
|
Zymed/ Invitrogen |
monoclonal
IgG1,κ |
Mouse
(ascites, PBS, azide, protein-A purifiedAlso available FITC conj; 33-9111) |
IF, WB |
Dog, Human |
immunogen = human recomb. ZO-1 fusion protein encompassing amino acids 334-634 |
reacts with the ZO-1α+ and ZO-1α- isoforms; is specific for ZO-1 proteins; cross-reactivity with the related ZO-2 protein has not been observed |
- |
|
|
Zymed/ Invitrogen |
monoclonal
IgG1,κ |
Mouse
(ascites, PBS, azide, protein-A purifiedAlso available FITC conj, 33-1511 andHRP conj, 33-1520) |
ELISA, IF, WB |
Dog, Human, Mouse, Rat |
immunogen = GST fusion protein consisting of the C-terminal (150 a.a.) of human occludin fused to GST |
reacts specifically with mammalian occludin |
- |
|
|
Zymed/ Invitrogen |
polyclonal
Ig |
Rabbit
(antiserum, PBS, azide, affinity purified) |
IF, IH, WB |
Human |
immunogen = synthetic peptide derived from the N-terminal region of the human, rat, mouse, dog, and orangutan occludin proteins |
reacts specifically with occludin |
- |
|
|
Zymed/ Invitrogen |
polyclonal
Ig |
Rabbit
(antiserum, PBS, azide, affinity purified) |
IF, IH, WB |
Dog, Human, Mouse, Rat |
immunogen = synthetic peptide derived from the C-terminal region of the human occludin protein |
reacts with occludin, ~65 kDa |
- |
|
|
Zymed/ Invitrogen |
polyclonal
Ig |
Rabbit
(antiserum, PBS, azide, affinity purified) |
IF, WB |
Dog, Human |
immunogen = GST-fusion protein containing the C-terminal region of human occludin protein |
reacts with occludin, ~65 kDa |
- |
|
|
Zymed/ Invitrogen |
polyclonal
Ig |
Rabbit
(antiserum, PBS, azide, affinity purified using a GST-Occludin coupled gel) |
ELISA, IF, IP, WB |
Dog, Human, Mouse, Rat |
immunogen = GST fusion protein consisting of the C-terminal 150 aa of human occludin fused to GST |
reacts specifically with mammalian occludin |
- |
|
|
Zymed/ Invitrogen |
polyclonal
Ig |
Rabbit
(antiserum, PBS, azide, affinity purified) |
ELISA, IH, IP, WB |
Dog, Human, Guinea Pig, Mouse, Rat |
immunogen = 69 kD fusion protein corresp. to aa 463-1109 of human ZO-1 cDNA; the sequence lies N-terminal to the 80 aa region (the α-motif) present in the a+ isoform but absent in the a- isoform due to alternative splicing |
reacts with the ZO-1α+ and ZO-1α- isoforms. Reactivity with dlg has not been characterized |
- |
|
|
Zymed/ Invitrogen |
polyclonal
Ig |
Rabbit
(antiserua, PBS, BSA, azide, purified) |
IH |
Human |
immunogen = peptide sequence from the N-terminal region of human ZO-1 |
staining of ZO-1, in membrane, and may display cytoplasmic staining in tumor cells |
- |
|
|
Zymed/ Invitrogen |
polyclonal
Ig |
Rabbit
(antiserum, PBS, azide, affinity purified) |
IF, IH, WB |
Dog, Human, Mouse, Rat |
immunogen = synthetic peptide derived from the N-terminal region of human, dog, mouse, and rat ZO-1 |
reacts with the ~225 kDa ZO-1 protein; bands of unknown origin may be observed at lower molecular weights. |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IC, IF, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide derived from within residues 1350 to the C-terminus of mouse Caspr, conjugated to KLH |
Caspr/NCP1 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, IF, WB |
Human, Mouse, Rat |
immunogen = epitope mapping near the N-terminus of human Caspr |
Caspr/NCP1 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, IF, WB |
Human, Mouse, Rat |
immunogen = epitope mapping near the C-terminus of human Caspr |
Caspr/NCP1 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, IF, WB |
Human, Mouse, Rat |
immunogen = epitope mapping near the N-terminus of human Caspr |
Caspr/NCP1 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, IF, WB |
Human, Mouse, Rat |
immunogen = epitope mapping within an internal region of human Caspr2 |
Caspr2 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = epitope corresp. to aa1251-1316 mapping near the C-terminus of human Caspr |
Caspr/NCP1 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, IF, WB |
Human |
immunogen = epitope mapping near the N-terminus of human Caspr2 |
Caspr2 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, IF, WB |
Human, Mouse, Rat |
immunogen = epitope mapping near the C-terminus of human Caspr2 |
Caspr2 |
- |
|
|
Novus Biologicals |
polyclonal
|
Mouse
(mouse antisera, glycerol, no preservative) |
ELISA, WB |
Human |
immunogen = OCLN (1 a.a. ~ 522 a.a) human protein |
reacts with occludin |
- |
|
|
Novus Biologicals |
monoclonal
IgG |
Rat
(ascites, azide) |
IH, IP, WB |
Dog, Porcine, Mouse, Rat |
immunogen = DOC-JR-plasma membranes from mouse liver |
reacts with ZO-1 (membrane associated, non-integralmembrane protein) |
- |
|
|
Invitrogen Corporation |
monoclonal
IgG1,κ |
Mouse
(affinity purified, liquid, PBS, azide) |
ELISA, IH, WB |
Human, Mouse, Rat |
immunogen = Synthetic peptide corresponding to a segment of the cytoplasmic loop of rat Connexin-26 |
monoclonal reacts strongly with Connexin-26, which has a predicted molecular weight of 26.5 kDa; anti-Cx26 appears to exhibit minimal cross reaction with Cx30 by western blotting |
- |
|
|
Invitrogen Corporation |
monoclonal
IgG2a,κ |
Mouse
(protein-A purified, liquid, PBS, azide) |
ELISA, IH, WB |
Human, Mouse, Rat |
immunogen = a 13 amino acid synthetic peptide derived from the C-terminus of the mouse Connexin 26 protein. This mouse sequence differs from the rat sequence by a single amino acid and from the human sequence by two (non-consecutive) amino acids |
monoclonal reacts with the Connexin 26 protein; no cross-reactivity with the closely related Connexin 30 protein has been observed. |
- |
|
|
Invitrogen Corporation |
monoclonal
IgG1 |
Mouse
(protein-A purified, liquid, PBS, azide) |
IF, WB |
Mouse, Rat |
immunogen = synthetic peptide derived from rat Connexin 43 |
monoclonal reacts specifically with both phosphorylated and non-phosphorylated forms of Connexin 43; does not react with the Connexin 43 dimer, but reacts with non phosphorylated forms migrating at ~41 kD and phosphorylated forms migrating between 43 kD and 45 kD |
- |
|
|
Invitrogen Corporation |
polyclonal
|
Rabbit
(antiserum, affinity purified, PBS, azide) |
IH, WB |
Mouse, Rat |
immunogen = a 13 amino acid synthetic peptide derived from the C-terminus of the mouse Connexin 26 protein. This mouse sequence differs from the rat sequence by a single amino acid and from the human sequence by two (non-consecutive) amino acids |
polyclonal detects the Connexin 26 protein; no cross-reactivity with the closely related Connexin 30 protein has been observed |
- |
|
|
Invitrogen Corporation |
polyclonal
|
Rabbit
(affinity purified, liquid, PBS, azide) |
ELISA, IF, IH, WB |
Mouse, Rat |
immunogen = peptide corresponding to a portion of the cytoplasmic loop of rat Connexin-26 |
polyclonal detects predominantly Connexin 26, 26 kDa; a few cross reactive bands were detected in the 45-50 kDa range |
- |
|
|
Invitrogen Corporation |
polyclonal
|
Rabbit
(affinity purified, liquid, HEPES, saline, BSA, glycerol) |
WB |
Rat |
immunogen = phosphopeptide corresp. to amino acid residues surrounding the phosphoserine 368 of rat connexin 43 |
polyclonal specific for the ~43 kDa connexin 43 protein phosphorylated at serine 368 |
- |
|
|
Invitrogen Corporation |
monoclonal
IgG1,κ |
Mouse
(affinity purified, liquid, PBS, azide) |
ELISA, EM, IF, IH, WB |
Dog, Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to a cytoplasmic sequence located near the C-terminus of rat Connexin 43 |
monoclonal CX-1B1 recognizes Connexin 43 only when the serine at residue 368 is unphosphorylated, but will recognize Connexin 43 if it is phosphorylated at sites besides Ser368 |
- |
|
|
Invitrogen Corporation |
monoclonal
IgG1,κ |
Mouse
(ascites, protein-A purified, liquid, PBS, BSA, azide, Alexa Fluor 488 conj.) |
ELISA, EM, IF, IH, WB |
Dog, Human, Mouse, Rat |
immunogen = synthetic peptide corresponding to a cytoplasmic sequence located near the C-terminus of rat Connexin 43 |
monoclonal CX-1B1 is specific for one of the unphosphorylated forms of Connexin 43; CX-1B1 recognizes Connexin 43 only when the serine at residue 368 is unphosphorylated. CX-1B1 will, however, recognize Connexin 43 if it is phosphorylated at sites besides Ser368. |
- |
|
|
Santa Cruz |
polyclonal
|
Rabbit
(purified, liquid, PBS, azide, gelatin) |
ELISA, IF, IH, IP, WB |
Human, Mouse |
immunogen = apeptide mapping near the C-terminus of connexin 26 of human origin |
polyclonal detects Connexin 26, 26 kDa |
- |
|
|
Santa Cruz |
polyclonal
|
Goat
(affinity purified, liquid, PBS, azide, gelatinAlso available as rabbit rabbit, sc-7261-R) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = a peptide mapping at the N-terminus of connexin 26 of human origin |
polyclonal for detection of connexin 26, and toa lesser extent, connexin 30 |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
Dog, Human, Mouse, Rat |
immunogen = aminoacids 241-254 within the cytoplasmic domain of connexin 43 of rat origin |
monoclonal CXN-6 is recommended for detection of connexin 43. |
- |
|
|
Santa Cruz |
monoclonal
IgM |
Mouse
(ascites, azide) |
IF, IH, IP, WB |
Cow, Human, Mouse, Rat |
immunogen = aminoacids 362-381 of connexin 43 of human origin |
monoclonal CXN-6 is recommended for detection of connexin 43. |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, affinity purified IgG, PBS, azide, gelatin.Also available sc-6560 AC, agarose conj.) |
ELISA, IF, IP, WB |
Dog, Zebra fish, Cow, Human, Mouse, Rat, Xenopus |
immunogen = a peptide mapping at the C-terminus of connexin 43 of human origin |
recommended for detection of connexin 43. |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid IgG, PBS, azide, gelatin) |
ELISA, IF, IP, WB |
Zebra fish, Human, Mouse, Rat, Xenopus |
immunogen = aminoacids 233-382 mapping at the C-terminus of connexin 43 of human origin |
recommended for detection of connexin 43. |
- |
|
|
Invitrogen Corporation |
polyclonal
|
Rabbit
(affinity purified, liquid, PBS, azide) |
ELISA, IH, WB |
Human, Mouse, Rat |
immunogen = peptide corresponding to a segment of the 3rd cytoplasmic domain (C-terminal portion) of rat connexin 43 |
polyclonal detects Connexin 43, 43 kDa; reactivity of this antibody with connexin 43 is independent of phosphorylation status |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(affinity purified, liquid, Tris, BSA, azide) |
ELISA, IC, IP, WB |
Bovine, Mouse, Rat |
immunogen = a 19 amino acid C-terminal peptide sequence within the cytoplasmic domain of mouse Cx43, coupled to KLH |
immunogenic peptide sequence is specific for Cx43 and no significant homology is seen with other connexins. The mouse Cx43 peptide sequence shows 100% conserved with rat and bovine, and 84% with chicken and human (16/19 aa). |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(purified, liquid, phosphate buffer, saline, azide) |
ELISA, IF, IH, IP, WB |
Human, Mouse, Rat |
immunogen = a 23 amino acid C-terminal peptide sequence within the cytoplasmic domain of mouse Cx43 |
immunogenic peptide sequence is specific for Cx43 and no significant homology is seen with other connexins. The mouse Cx43 peptide sequence is 100% homologous with rat and human Cx43 |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(affinity purified, liquid, Tris, BSA, azide) |
ELISA, IH, WB |
Mouse |
immunogen = a 19 amino acidpeptide sequence located near the C-terminal, second cytoplasmic domain of mouse Cx26; conjugated to KLH as a carrier protein |
specific for Connexin-26 |
- |