|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum, PBS, BSA, azide) |
IP, WB |
Human |
Immunogen = two synthetic peptides, conjugated to KLH, derived from within residues 1 - 50 and 450-610 of human SIRT1 |
SIRT1 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IF, WB |
Mouse |
Immunogen = synthetic peptide: A T R Q E L T D V N Y P S D K S (with C-terminal added lysine), corresp. to C terminal aa 722-737 of mouse SIRT1 |
SIR2 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide, affinity purified) |
WB |
Human |
Immunogen = peptide mapping to the C-terminal domain of human SIRT1 |
SIRT1 |
- |
|
|
ABGENT |
polyclonal
Ig |
Rabbit
(liquid, PBS, azide, Protein G purified) |
ELISA |
Human |
Immunogen = synthetic peptide (10-30 aa in length) at the C-term of last 100 aa of human Sirt1, KLH coupled |
SIRT1 |
- |
|
|
Biomol Enzo Life Sciences |
polyclonal
|
Rabbit
(liquid, immuno-depleted antiserum, PBS) |
WB |
Human |
Immunogen = recombinant human SIRT1 |
SIRT1 (recombinant and native). No cross-reactivity with recombinant human SIRT2, SIRT3 or SIRT5. |
- |
|
|
AMS Biotechnology |
polyclonal
Ig |
Rabbit
(liquid, PBS, azide, Protein G purified) |
ELISA |
Human |
Immunogen = synthetic peptide (10-30 aa in length) at the C-term of last 100 aa of human Sirt1, KLH coupled |
SIRT1 |
- |
|
|
Autogen Bioclear |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
IF, IP, WB |
Human, Mouse, Rat |
Immunogen = amino acids 448-747 of human SIRT1 |
SIRT1 |
- |
|
|
Delta Biolabs |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide, affinity purified) |
WB |
Human |
Immunogen = peptide mapping to the C-terminal domain of human SIRT1 |
SIRT1 |
- |
|
|
GeneTex |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IF, WB |
Mouse |
Immunogen = synthetic peptide: A T R Q E L T D V N Y P S D K S (with C-terminal added lysine), corresp. to C terminal aa 722-737 of mouse SIRT1 |
SIR2 |
- |
|
|
GeneTex |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum, PBS, BSA, azide) |
IP, WB |
Human |
Immunogen = two synthetic peptides, conjugated to KLH, derived from within residues 1 - 50 and 450-610 of human SIRT1 |
SIRT1 |
- |
|
|
GeneTex |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide, affinity purified) |
WB |
Human |
Immunogen = peptide mapping to the C-terminal domain of human SIRT1 |
SIRT1 |
- |
|
|
IBL |
polyclonal
IgG |
Rabbit
(purified, BSA, PBS, azide, lyophilized) |
IH, IP, WB |
Human |
Immunogen = Synthetic peptides for part of C Terminal of human Sir2/SIRT1(LEDEPDV PERAGG) |
Sir2/SIRT1 |
- |
|
|
I B L America |
polyclonal
IgG |
Rabbit
(affinity purified, BSA, PBS, azide, lyophilized) |
IH, IP, WB |
Human |
Immunogen = Synthetic peptides for part of C Terminal of human Sir2/SIRT1(LEDEPDV PERAGG) |
Sir2/SIRT1 |
- |
|
|
Insight Biotechnology |
polyclonal
IgG |
Rabbit
(PBS, azide, gelatin) |
IF, IP, WB |
Human, Mouse, Rat |
immunogen = aa 448-747 of SIRT1 of human origin |
SIRT1 |
- |
|
|
Insight Biotechnology |
polyclonal
IgG |
Goat
(PBS, azide, gelatin, affinity purified) |
IF, IP, WB |
Human, Mouse, Rat |
immunogen = peptide mapping within an internal region of SIRT1 of human origin |
SIRT1 |
- |
|
|
Insight Biotechnology |
polyclonal
IgG |
Goat
(PBS, azide, gelatin, affinity purified) |
IF, WB |
Human |
immunogen = peptide mapping near the C-terminus of SIRT1 of human origin |
SIRT1 |
- |
|
|
Epitomics |
monoclonal
IgG |
Rabbit
(liquid, tris-glycine saline, glycerol, azide, BSA) |
IC, IH, IP, WB |
Human |
immunogen = synthetic peptide corresp. to residues in C-terminus of human Sir2/SIRT1 |
SIRT1; no cross-reaction with other sirtuin family members |
- |
|
|
Epitomics |
monoclonal
IgG |
Rabbit
(liquid, tris-glycine saline, glycerol, BSA, azide) |
FC, IC, IH, IP, WB |
Human |
immunogen = synthetic peptide corresp. to residues near N-terminus and before Ala-rich domain of human Sir2 |
Sir2/SIRT1 |
- |
|
|
Insight Biotechnology |
monoclonal
IgG |
Rabbit
(liquid, tris-glycine saline, glycerol, azide, BSA) |
IC, IH, IP, WB |
Human |
immunogen = synthetic peptide corresp. to residues in C-terminus of human Sir2/SIRT1 |
SIRT1; no cross-reaction with other sirtuin family members |
- |
|
|
Insight Biotechnology |
monoclonal
IgG |
Rabbit
(liquid, tris-glycine saline, glycerol, BSA, azide) |
FC, IC, IH, IP, WB |
Human |
immunogen = synthetic peptide corresp. to residues near N-terminus and before Ala-rich domain of human Sir2 |
Sir2/SIRT1 |
- |
|
|
Insight Biotechnology |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide, affinity purified) |
WB |
Human |
immunogen = peptide mapping to the C-terminal domain of human SIRT1 |
SIRT1 |
- |
|
|
Sigma Aldrich |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide) |
ELISA, WB |
Mouse |
immunogen = synthetic peptide corresp. to aa. 722-737 at the C-terminus of mouse Sir2 with C-terminal added lysine |
SIRT1 |
- |
|
|
Interchim |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
IF, WB |
Mouse |
Immunogen = synthetic peptide: A T R Q E L T D V N Y P S D K S (with C-terminal added lysine), corresp. to C terminal aa 722-737 of mouse SIRT1 |
SIR2 |
- |
|
|
Interchim |
polyclonal
|
Rabbit
|
ELISA |
Human |
Immunogen = within aa 646-660 |
- |
- |
|
|
Acris Antibodies |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide, affinity purified) |
WB |
Human |
Immunogen = peptide mapping to the C-terminal domain of human SIRT1 |
SIRT1 |
- |
|
|
MBL International |
polyclonal
IgG |
Rabbit
(DEAE purified, phosphate buffer, glycerol) |
IP |
Human |
Immunogen = recombinantfull length SIRT |
detects endogenous levels of SIRT1 protein; does not cross-react with type I and II HDAC proteins. |
- |
|
|
Orbigen |
polyclonal
|
Rabbit
(liquid, PBS, azide, protein-G purified) |
ELISA |
Human |
Immunogen = KLH conjugated synthetic peptide comprised of aa 646 - 660 [PPNRYIFHGAEVYSD] of human SIRT1 |
SIRT1 |
- |
|
|
tebu-bio |
polyclonal
|
Rabbit
(liquid, immuno-depleted antiserum, PBS) |
WB |
Human |
Immunogen = recombinant human SIRT1 |
SIRT1 (recombinant and native). No cross-reactivity with recombinant human SIRT2, SIRT3 or SIRT5. |
- |
|
|
tebu-bio |
polyclonal
IgG |
Goat
(PBS, azide, gelatin, affinity purified) |
IF, WB |
Human |
immunogen = peptide mapping near the C-terminus of SIRT1 of human origin |
SIRT1 |
- |
|
|
tebu-bio |
polyclonal
IgG |
Goat
(PBS, azide, gelatin, affinity purified) |
IF, IP, WB |
Human, Mouse, Rat |
immunogen = peptide mapping within an internal region of SIRT1 of human origin |
SIRT1 |
- |
|
|
tebu-bio |
polyclonal
IgG |
Rabbit
(PBS, azide, gelatin) |
IF, IP, WB |
Human, Mouse, Rat |
immunogen = aa 448-747 of SIRT1 of human origin |
SIRT1 |
- |
|
|
Biomol Enzo Life Sciences |
kit
|
Unknown Host
(complete assay system designed to measure the lysyldeacetylase activity of recombinant human SIRT1) |
IF |
- |
- |
SIRT1, recombinant |
- |
|
|
tebu-bio |
kit
|
Unknown Host
(complete assay system designed to measure the lysyldeacetylase activity of recombinant human SIRT1) |
IF |
- |
- |
SIRT1, recombinant |
- |
|
|
US Biological |
monoclonal
IgG1 |
Mouse
(liquid, PBS, protein G affinity purified) |
ELISA, IP, WB |
Human |
immunogen = synthetic peptide corresponding to aa 551–599 of human silent information regulator 2 homolog (Sirt1) protein, ovalbumin-conj. |
Sirt1 (120kDa) |
- |
|
|
US Biological |
polyclonal
IgG |
Rabbit
(liquid, serum) |
WB |
Human |
immunogen = a mixture of two peptides: 1. G A D R E A A S S P A G E P (aa 22-32) 2. M E N P D L K N V G S S T G E K (aa 595-610), KLH-conjugated; GenBank# NP_036370 |
Sirt1 |
- |
|
|
US Biological |
monoclonal
IgG1 |
Mouse
(liquid, PBS, glycerol, azide, affinity purified) |
IP, WB |
Human |
immunogen = synthetic peptide corresponding to aa551-599 of human Sirt1, ovalbumin-conjugated |
Sirt1 (120kDa and unidentified band at 100kDa) |
- |
|
|
Austral Biologicals |
monoclonal
IgG1 |
Rabbit
(liquid, IgG fraction, Protein G purified) |
ELISA, IB, IP |
Human |
Immunogen = synthetic peptide corresp. to aa 551-599 of human Sirt1, ovalbumin conj. |
120 kDa SIRT1 |
- |
|
|
Abcam |
monoclonal
IgG |
Rabbit
(liquid, tris-glycine, BSA, glycerol, azide) |
IC, IH, IP, WB |
Human |
immunogen = synthetic peptide corresp. to residues in C-terminus of human Sir2/SIRT1 |
SIRT1; no cross reaction with sirtuin family members |
- |
|
|
Abcam |
monoclonal
IgG |
Rabbit
(liquid, tris-glycine saline, BSA, glycerol, azide) |
FC, IC, IH, IP, WB |
Human |
immunogen = synthetic peptide corresponding to residues near N-terminus and before Ala-rich domain of human Sir2. |
SIRT1; no cross-reaction with other sirtuin family members |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
WB |
Mouse |
immunogen = synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of mouse SIRT1 |
SIRT1 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(PBS, azide, gelatin, affinity purified) |
IF, WB |
Human |
immunogen = peptide mapping near the C-terminus of SIRT1 of human origin |
SIRT1 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(PBS, azide, gelatin, affinity purified) |
IF, IP, WB |
Human, Mouse, Rat |
immunogen = peptide mapping within an internal region of SIRT1 of human origin |
SIRT1 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(PBS, azide, gelatin) |
IF, IP, WB |
Human, Mouse, Rat |
immunogen = aa 448-747 of SIRT1 of human origin |
SIRT1 |
- |
|
|
Novus Biologicals |
monoclonal
IgG1,κ |
Mouse
(liquid, purified IgG, no preservative) |
WB |
Human |
Immunogen = partial recomb. SIRT1 (456 aa. ~ 555 aa.) with GST tag |
SIRT1 |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, TBS, BSA, azide, affinity purified) |
IP, WB |
Human |
Immunogen = peptide mapping to a region between residue 25 and 75 of human SIRT1 |
Sirt1, nuclear |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, TBS, BSA, azide, affinity purified) |
IP, WB |
Human |
Immunogen = peptide mapping to a region between residue 700 and the C-terminus (residue 747) of human SIRT1 |
Sirt1, nuclear |
- |
|
|
Novus Biologicals |
polyclonal
|
Mouse
(liquid, antisera, glycerol, no preservative) |
IP, WB |
Human |
Immunogen = partial recomb. SIRT1 |
SIRT1 |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, PBS, glycrol, azide, Protein G purified) |
IP, WB |
Human |
Immunogen = peptide corresp. to aa 551-599 of human Sirt1 |
SIRT1(120kDa by WB). An additional unidentified band was detected (100 kDa). |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG |
Rabbit
(liquid, Tris-Glycine, saline, BSA, glycrol, azide) |
IC, IH, IP, WB |
Human |
Immunogen = synthetic peptide corresponding to residues near C-terminus and before Ala-rich domain of human Sir2 |
recognizes SIRT1(~110 kDa by WB); does not cross-react with other sirtuin family members. |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG,κ |
Mouse
(liquid, purified, Tris-Glycine, saline, azide) |
IC, IH, IP, WB |
Human, Mouse |
Immunogen = His-tagged recombinant protein corresponding to human SIRT1; mouse sequence is 100% identical to immunogen |
recognizes SIRT1 |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
IgG |
Rabbit
(liquid, purified, Tris-Glycine, saline, azide, glycerol) |
IC, IP, WB |
Human, Mouse |
Immunogen = His-tagged fusion protein corresponding to amino acids 1-131 of mouse Sir2α |
recognizes SIRT1, ~110 kDa |
- |
|
|
None |
polyclonal
|
Rabbit
(liquid, affinity purified, PBS, azide, gelatin) |
IP, WB |
Human |
immunogen = peptide mapping to the carboxy terminal domain of human SIRT1 |
recognizes human SIRT1 protein |
Discontinued |