|
|
DSHB U. Iowa |
monoclonal
IgG1,κ |
Mouse
|
IH |
Drosophila |
immunogen = homogenates of adult drosophilia fly heads |
recognizes Futsch protein in axons |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, PBS,BSA, azide) |
IH, IP, WB |
Rabbit, Chicken, Pig, Human, Guinea Pig, Mouse, Rat |
Immunogen = purified Chicken red blood cell membranes |
240 kDa α-II-spectrin/fodrin of all mammalian nonerythroid cells and chicken alpha-spectrin. Antibody will all recognize caspase (150kDa, 120kDa) and calpain cleavage (150kDa) products of alpha fodrin |
- |
|
|
Santa Cruz |
monoclonal
IgG2a |
Mouse
(liquid IgG, PBS, azide, gelatin; Available as agarose conjugate for IP, sc-17839 AC) |
IF, IP, WB |
Human, Mouse, Rat |
immunogen = amino acids 1-300 of Arc of human origin |
55 kDa Activity- regulated cytoskeleton- associated protein |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG2a |
Rabbit
(liquid, PBS, BSA, azide) |
IH |
Human |
immunogen = peptide corresp. to the C-terminus of human ARC |
Activity- regulated cytoskeleton- associated protein (Arc) |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG2a |
Rabbit
(liquid, PBS, azide) |
WB |
Human, Mouse, Rat |
immunogen = peptide corresp. to aa2-18 of human ARC |
Activity- regulated cytoskeleton- associated protein (Arc) |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG2a |
Rabbit
(liquid, PBS, BSA, glycerol, thimerosal, affinity) |
WB |
Human, Mouse, Rat |
immunogen = synthetic peptide mapping to the N-terminus of human ARC |
Activity- regulated cytoskeleton- associated protein (Arc) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = epitope mapping at the C-terminus of Arc of rat origin |
Activity- regulated cytoskeleton- associated protein (Arc) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, IF, WB |
Human, Mouse, Rat |
immunogen = epitope mapping at the N-terminus of Arc of rat origin |
Activity- regulated cytoskeleton- associated protein (Arc) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, IF, WB |
Human, Mouse, Rat |
immunogen = epitope mapping within an internal region of Arc of human origin |
Activity- regulated cytoskeleton- associated protein (Arc) |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin) |
ELISA, IF, IH, IP, WB |
Human, Mouse, Rat |
immunogen = epitope corresp. to aa1-300 of Arc (activity-regulated cytoskeleton-associated protein) of human origin |
Activity- regulated cytoskeleton- associated protein (Arc) |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG2a |
Mouse
(liquid, PBS, azide, purified) |
ELISA, FC, IC, IH, IP, RIA, WB |
Bovine, Dog, Porcine, Rabbit, Human, Mouse, Rat |
Immunogen = purified bovine brain fodrin |
reacts with the intracellular fodrin (240 kDa) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, azide, antiserum) |
IC, IF, WB |
Mammal (various), Avian (various) |
Immunogen = full length native protein (purified spectrin α and β chain from chicken) |
detect spectrin and spectrin-like molecules |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
IC, IF, WB |
Human |
Immunogen = Full length purified native human erythroid spectrin |
localizes the 220 kDa (beta) and 240 kDa (alpha) bands of human erythrocyte spectrin as well as a number of polypeptides in the region of 170, 150, 85, and 45 kDa |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, ascites, azide) |
IEP |
Human |
Immunogen = SDS-PAGE purified spectrin (α and β) from freshly prepared human erythrocyte ghosts |
detect spectrin and spectrin-like molecules |
- |
|
|
Abcam |
monoclonal
IgM |
Mouse
(liquid, PBS, azide) |
ELISA |
Human |
Immunogen = native β spectrin |
Spectrin (α + β) |
- |
|
|
Abcam |
monoclonal
IgG2b |
Mouse
(liquid, supernatant, azide) |
IH |
Human |
Immunogen = human red blood cell membrane 'ghosts' |
recognizes the β chain of spectrin in human red blood cells and muscle |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, ppt supernatant) |
ELISA, IC, IF, IH, WB |
Human, Mouse, Rat |
Immunogen = recombinant α I spectrin (erythroid spectrin) SH3 domain |
alpha I spectrin SH3 domain; no crossreaction with α II (nonerythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In NIH 3T3 cells, reacts with a 200 kDa protein which is a candidate marker of endocytic vesicles called macropinosomes |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, ascites) |
IF, WB |
Human, Mouse, Rat |
Immunogen = purified human erythrocyte β I spectrin |
spectrin from erythrocytes, brain and muscle cells; specifically detects the two forms of spectrin, β-1 epsilon-1, present in erythrocytes, and β-1 ε-2, present in nerve and striated muscle cells; no cross-reaction with α-2 spectrin or either of the fodrin subunits |
- |
|
|
Abcam |
monoclonal
IgG2b |
Mouse
(liquid, PBS, supernatant, azide) |
ELISA, IC, IF, IH, IP, WB |
Human, Mouse, Rat, African green monkey, Chinese hamster |
Immunogen = recombinant α Fodrin (non-erythroid spectrin) SH3 domain (human) |
α-II spectrin SH3 domain; nocrossreaction with α-I (erythroid) spectrin SH3 domain |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(lyophilized, PBS, BSA, azide, affinity purified) |
IC, IH, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide corresp. to a sequence at the C-terminus of human SSH3BP1 |
SSH3BP1 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, TBS, BSA, azide, affinity purified) |
IP, WB |
Human |
Immunogen = synthetic peptide corresp. to a region between aa325-375 of human SSH3BP1 (NP_005461.2) |
SSH3BP1 |
- |
|
|
Abcam |
monoclonal
IgG |
Rabbit
(liquid, BSA, supernatant, azide, glycerol, Tris-glycine saline) |
IC, WB |
Human, Mouse, Rat |
Immunogen = synthetic peptide corresp. to residues in human α Fodrin |
Alpha Fodrin |
- |
|
|
Abcam |
monoclonal
IgG2b |
Mouse
(liquid, supernatant, PBS, azide, Ammonium sulfate ppt) |
IC, IF, IH, WB |
Human, Mouse, Rat |
Immunogen = recombinant full length protein (human) |
SSH3BP1 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, glycerol, affinity purified) |
ELISA |
Human |
Immunogen = synthetic peptide corresp. to residues in human α Fodrin |
detects endogenous levels of fragment of activated Alpha Fodrin resulting from cleavage adjacent to Asp1185 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, glycerol, affinity purified) |
IH, WB |
Human |
Immunogen = synthetic peptide surrounding aa1188 in human α Fodrin |
recognizes the 240 kDa full length and 150 kDa N-terminal fragment of alpha Fodrin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, Tris citrate/phosphate, azide, affinity purified) |
WB |
Human, Mouse |
Immunogen = synthetic peptide from a region between residues 1000-1050 in human α Fodrin, (NP_003118.1) |
recognizes the 240 kDa full length and 150 kDa N-terminal fragment of alpha Fodrin |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, Tris citrate/phosphate, azide, affinity purified) |
WB |
Human, Mouse |
Immunogen = synthetic peptide from a region between residues 2422-2472 in human α Fodrin, (NP_003118.1) |
recognizes the 240 kDa full length and 150 kDa N-terminal fragment of alpha Fodrin |
- |
|
|
Abcam |
monoclonal
IgG3, κ |
Mouse
(liquid, PBS, no preservative, protein G purified) |
WB |
Human |
Immunogen = recombinant fragment corresp. to aa643-721 of human SPTBN2 |
SPTBN2 (Spectrin beta non erythrocytic 2) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid,Tris-citrate /phosphate, azide, affinity purified) |
IH, IP, WB |
Human |
Immunogen = synthetic peptide corresp. to a region between aa 2300 and 239 of Human SPTBN2 (C-terminal) |
SPTBN2 (Spectrin beta non erythrocytic 2) plays an important role in neuronal membrane skeleton. It is highly expressed in Purkinje cells and stabilizes the glutamate transporter EAAT4 at the surface of the plasma membrane |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid,Tris-citrate /phosphate, azide, affinity purified) |
IH, IP |
Human |
Immunogen = synthetic peptide corresp. to a region between aa 2150 and 2200 of human SPTBN2 |
SPTBN2 (Spectrin beta non erythrocytic 2) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, TBS, BSA, azide, affinity purified) |
IP, WB |
Human, Mouse |
Immunogen = synthetic peptide from a region between residue 2100-2150 of human SPTBN1 (NP_003119.2) |
SPTBN1 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, glycerol, affinity purified) |
ELISA, WB |
Human, Mouse |
Immunogen = synthetic phospho-peptide (p-tyrosine 435) corresp. to C- terminal residues of human SSH3BP1 |
SSH3BP1 phosphorylated at Tyr435 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, TBS, BSA, azide, affinity purified) |
IH |
Human |
Immunogen = synthetic peptide corresp. to a region between aa325-375 of human SSH3BP1 (NP_005461.2) |
SSH3BP1 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, azide, affinity purified) |
ELISA, WB |
Human, Mouse |
Immunogen = synthetic peptide corresp. to C-terminal residues of human SSH3BP1 |
SSH3BP1 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, Tris citrate/phosphate, azide, affinity purified) |
IP |
Human |
Immunogen = synthetic peptide corresp. to a region between residue 150-200 of human SSH3BP1 (NP_005461.2) |
SSH3BP1 |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(liquid, Tris-citrate/phosphate buffer, azide, affinity purified) |
IH, IP |
Human, Mouse, Rat |
Immunogen = maps to a region between aa2150-2200 of human spectrin, β, non-erythrocytic 2 (spinocerebellar ataxia 5 ) using the numbering given in entry NP_008877.1 |
SPTBN2 (Spectrin beta non erythrocytic 2) |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(liquid, Tris-citrate/phosphate buffer, azide, affinity purified) |
IH, IP, WB |
Human, Mouse, Rat |
Immunogen = maps to a C-terminal region between aa2300-2390 of human spectrin, β, non-erythrocytic 2 (spinocerebellar ataxia 5) using the numbering given in entry NP_008877.1 |
SPTBN2 (Spectrin beta non erythrocytic 2) |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(liquid, Tris-citrate/phosphate buffer, azide, affinity purified) |
IH, IP |
Human, Mouse, Rat |
Immunogen = maps to a region between aa2150-2200 of human spectrin, β, non-erythrocytic 2 (spinocerebellar ataxia 5) using the numbering given in entry NP_008877.1 |
SPTBN2 (Spectrin beta non erythrocytic 2) |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(liquid, Tris-citrate/phosphate buffer, azide, affinity purified) |
IH, IP, WB |
Human, Mouse, Rat |
Immunogen = maps to a C-terminal region between aa2300-2390 of human spectrin, β, non-erythrocytic 2 (spinocerebellar ataxia 5) using the numbering given in entry NP_008877.1 |
SPTBN2 (Spectrin beta non erythrocytic 2) |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, TBS, BSA, azide, affinity purified) |
IP, WB |
Human, Mouse |
Immunogen = maps to a region between aa2100-2150 of human SPTBN1 (NP_003119.2) (GeneID 6711) |
SPTBN1 |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, TBS, BSA, azide, affinity purified) |
IH |
Human |
Immunogen = maps to a region between aa2150-2200 of human SPTBN1 (NP_003119.2) (GeneID 6711) |
SPTBN1 |
- |
|
|
Novus Biologicals |
monoclonal
IgM |
Mouse
(liquid, ascites, azide) |
IF, IH, WB |
Human |
- |
localizes the 220 kDa (beta) and 240 kDa (alpha) bands of human erythrocyte spectrin as well as a number of polypeptides in the region of 170, 150, 85, and 45 kDa |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(lyophilized, antiserum) |
IF, WB |
Bovine, Rabbit, Human, Monkey, Hamster, Mouse |
Immunogen = native, from RBCs |
reacts with spectrin from erythrocytes, lymphocytes, macrophages, granulocytes, fibroblasts, epithelial cells, glial cells and neuronal cells |
- |
|
|
Novus Biologicals |
monoclonal
IgM |
Mouse
(liquid, PBS, azide, purified) |
ELISA, IH |
Human |
Immunogen = native human β spectrin |
recognises α (240 kD) and β (220 kD) subunits of human spectrin |
- |
|
|
Novus Biologicals |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, BSA, azide, protein G purified) |
IH |
Cow, Human, Rat |
immunogen = full length native protein (purified) (cow) |
recognizes MAP1b |
- |
|
|
Novus Biologicals |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
IH, WB |
Cat, Chicken, Cow, Human, Hamster, Mouse, Rat |
immunogen = rat brain microtubule associated proteins |
recognizes MAP1b; does not react with tubulin of other microtubule associated proteins |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, Tris-citrate buffer, azide, affinity purified) |
IP |
Human |
immunogen = maps to a region between aa900-950 of human microtubule-associated protein 1B [NP_005900.2 (GeneID 4131)] |
recognizes MAP1b |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, Tris-citrate buffer, azide, affinity purified) |
IP |
Human |
immunogen = maps to a region between aa2050-2100 of human microtubule-associated protein 1B [NP_005900.2 (GeneID 4131)] |
recognizes MAP1b |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, PBS, protein G purified) |
IF, IH |
Bovine, Human, Rat |
immunogen = purified bovine brain MAPs |
recognizes MAP1b; no reaction with tubulin, tau or other microtubule associated proteins (MAPs) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
IgG1 |
Mouse
(liquid, PBS, BSA, azide, protein G purified) |
IF, IH, IP, WB |
Bovine, Human, Rat |
immunogen = purified bovine brain MAPs |
recognizes MAP1b; no reaction with tubulin, tau or other microtubule associated proteins (MAPs) |
- |
|
|
LifeSpan Biosciences, Inc. |
monoclonal
|
Mouse
(liquid, PBS, BSA, azide, affinity purified) |
IF, IH |
Bovine, Human, Rat |
immunogen = purified bovine brain MAPs |
recognizes MAP1b; no reaction with tubulin, tau or other microtubule associated proteins (MAPs) |
- |
|
|
Santa Cruz |
polyclonal
IgG2a |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, IF, WB |
Human, Mouse, Rat |
immunogen = mapping near the C-terminus of human MAP-1B |
recognizes MAP-1A and MAP-1B |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IH, WB |
Human, Mouse, Rat |
immunogen = rat brain microtubule associated proteins |
recognizes MAP1b; does not react with tubulin of other microtubule associated proteins |
- |
|
|
Santa Cruz |
polyclonal
|
Rabbit
(liquid, PBS, azide, gelatin) |
ELISA, IF, IH, IP, WB |
Human, Mouse, Rat |
immunogen = corresp. to aa2221-2350 mapping near the C-terminus of human MAP-1B |
recognizes MAP-1B |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = mapping near the N-terminus of human MAP-1B |
recognizes MAP-1B |
- |
|
|
Santa Cruz |
monoclonal
IgG2a |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
Rat |
immunogen = taxol-stabilized microtubules derived from rat brain |
recognizes MAP1b |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, PBS, BSA, azide, protein G purified) |
IC, IF, IH |
Cow, Human, Mouse, Rat |
immunogen = purified bovine brain MAPs |
recognizes MAP1B |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
IC, IF, WB |
Cat, Chicken, Cow, Human, Hamster, Mouse, Rat |
immunogen = rat brain microtubule associated proteins |
recognizes MAP1B; no reaction with tubulin of other microtubule associated proteins |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(lyophilized, BSA, Na acetate, azide, affinity purified) |
IH, WB |
Rat |
immunogen = rat brain microtubule associated proteins |
MAP1a and MAP1b |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, BSA, azide, protein G purified, prediluted) |
IH |
Cow, Human, Rat |
immunogen = full length native protein (purified) (Cow) |
recognizes MAP1B |
- |
|
|
DSHB U. Iowa |
monoclonal
IgG2b,κ |
Mouse
|
IB |
Mammal (various), Avian (various), Amphibian (various) |
Immunogen = adult chicken pectoralis myosin, 200 kDa MHC peptide |
reacts with all sarcomere myosin |
- |
|
|
Cell Signaling |
polyclonal
|
Rabbit
(liquid, HEPES, saline, BSA, glycerol, affinity purified) |
WB |
Bovine, Human, Monkey, Hamster, Mouse, Rat |
immunogen = synthetic peptide (KLH-coupled)corresp. to residues surrounding Ser3 of human cofilin |
detects endogenous levels of total cofilin protein, 19 kDa by WB; may also recognize cofilin 2, the muscle isoform |
- |
|
|
Cell Signaling |
polyclonal
|
Rabbit
(liquid, HEPES, saline, BSA, glycerol, affinity purified) |
WB |
Bovine, Human, Monkey, Mouse, Rat |
immunogen = synthetic peptide (KLH-coupled)corresp. to residues surrounding Asp59 of human cofilin |
detects endogenous levels of total cofilin protein, 19 kDa by WB |
- |
|
|
Cell Signaling |
polyclonal
IgG |
Rabbit
(liquid, HEPES, saline, BSA, glycerol, affinity purified) |
WB |
Bovine, Human, Monkey, Mouse, Rat |
immunogen = synthetic phospho-peptide (KLH-coupled)corresp. to residues surrounding Ser3 of human cofilin |
recognizes endogenous levels of cofilin only when phosphor. at serine 3, 19 kDa by WB; may cross-react with phosphor. cofilin 2, the muscle isoform |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide, affinity purified) |
IF, WB |
Bovine, Human, Monkey, Mouse, Rat |
immunogen = synthetic phospho-peptide (KLH-coupled)corresp. to residues surrounding Ser3 of human cofilin |
recognizes endogenous levels of cofilin only when phosphor. at serine 3; 19 kDa by WB |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(liquid, serum, PBS, azide) |
IH, WB |
Mammal (various) |
Immunogen = brain spectrin isolated from enriched synaptic/axonal membranes from perfused mouse brain |
Detects brain spectrin (240/235) in the plasma membrane and cytoplasm of axons and presynaptic elements of neurons, where it is associated with microtubules, synaptic vesicles and mitochondria. No reactivity to erthrocyte subtype found in cell bodies and dendrites but not in axons |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = epitope corresp. to aa1-166 representing full length of human cofilin |
Cofilin 1 and Cofilin 2 and to a lesser extent, Destrin |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = epitope corresp. to phosphorylated Ser 3 of human cofilin |
detection of Ser 3 phosphorylated Cofilin |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin, affinity purified) |
IF, IP, WB |
Human, Mouse, Rat |
immunogen = short amino acid seq. containing phosphorylated Ser 3 of mouse Cofilin |
detection of Ser 3 phosphorylated Cofilin |
- |
|
|
Santa Cruz |
monoclonal
IgG2b |
Mouse
(liquid, PBS, azide, gelatin) |
ELISA, IP, WB |
Cow, Pig, Human, Mouse, Rat |
immunogen = full lenth human cofilin 1 |
Cofilin 1 |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
ELISA, IF, IH, IP, WB |
Human |
immunogen = recombinant human cofilin 2 |
Cofilin 2 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin) |
ELISA, IF, IH, IP, WB |
Mammal (various), Pig, Human, Mouse, Rat |
immunogen = epitope mapping near the C-terminus of human Cofilin 2 |
Cofilin 2, muscle isoform; no cross-reaction with Cofilin 1, non-muscle isoform |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, IF, IH, IP, WB |
Mammal (various), Cow, Pig, Human, Mouse, Rat |
immunogen = epitope mapping near the N-terminus of human Cofilin 1 |
Cofilin 1, non-muscle isoform; no cross-reaction with Cofilin 2, muscle isoform; may cross react with ADF |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, azide, gelatin, affinity purified) |
ELISA, IF, IP, WB |
Cow, Pig, Human, Mouse, Rat |
immunogen = peptide mapping at the C-terminus of human cofilin 1 |
Cofilin 1, non-muscle isoform; no cross-reaction with Cofilin 2, muscle isoform |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, glycerol, affinity purified) |
WB |
Dog, Human, Mouse, Rat |
immunogen = synthetic phosphopeptide derived from a region of human cofilin that contains serine 3 |
cofilin phosphorylated at Ser3 |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, TBS, azide, glycerol, protein A purified) |
WB |
Chicken, Mouse, Rat |
immunogen = synthetic peptide corresp. to aa136-152(C-Q V N G L D D I K D R S T L G E K) of human Cofilin 2 with N-terminal cysteine for KLH conj. |
recognizes human Cofilin 2, Mr 21kD. |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, TBS, azide, glycerol, protein A purified) |
ELISA, WB |
Chicken, Human, Mouse |
immunogen = synthetic peptide corresponding to aa1-10 (M A [pS] G V T V N D E-C) of human phospho-Cofilin 2, with a C-terminal cysteine added for KLH conj. |
recognizes phospho-Cofilin 2, Mr 21kD |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Rabbit
(liquid, TBS, BSA, azide, affinity purified) |
ELISA, WB |
Dog, Sheep, Pig, Mouse, Rat |
immunogen = synthetic peptide corresp. to C- terminus of Cofilin 2 (muscle): peptide sequence (D I K D R S T L G E K) |
recognizes human Cofilin 2, Mr 21kD. |
- |
|
|
AnaSpec |
polyclonal
|
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
ELISA, IH, WB |
Human |
Immunogen = a synthetic peptide corresponding to the C-terminus of human ARC protein |
detects ARC (Apoptosis Repressor with CARD) |
- |
|
|
AnaSpec |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
ELISA, WB |
Human, Mouse, Rat |
Immunogen = a peptide corresp. to amino acids 2-18 of ARC of human origin |
detects ARC (Apoptosis Repressor with CARD) |
- |
|
|
Novus Biologicals |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, no preservative) |
ELISA, IF, IH, WB |
Human |
immunogen = CFL2 (NP_068733, aa57-167 a.a) partial recombinant protein with GST tag. |
Cofilin 2 |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(liquid, PBS, no preservative) |
IF, IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide: C-D I K D R S T L G E K, representing the C-terminus of the human protein according to NP_068733.1; NP_619579.1 |
detects endogenous levels of Cofilin protein |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Goat
(liquid, TBS, BSA, azide) |
ELISA, WB |
Dog, Sheep, Pig, Human, Mouse, Rat |
immunogen = synthetic peptide: C-D I K D R S T L G E K, representing the C-terminus of the human protein according to NP_068733.1; NP_619579.1 |
recognize both reported isoforms (NP_068733 and NP_619579) |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, affinity purified) |
IH, WB |
Human, Mouse, Rat |
- |
Cofilin phos ser3 |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, glycerol, affinity purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthesized non-phosphopeptide derived from human cofilin around the phosphorylation site of serine 3 (M-A-SP-G-V) |
cofilin (specific to a.a. 1022) detects endogenous levels of total cofilin protein |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, glycerol, affinity purified) |
IF, WB |
Dog, Chicken, Pig, Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to aa154-166 of human cofilin with N-terminal cysteine added, conjugated to KLH |
cofilin (19kDa) |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(lyophilized, whole antiserum, no preservative) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide from the phospho Y140/S156 of human Cofilin-1 (Cofilin, non-muscle isoform) |
Cofilin 1 |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(lyophilized, whole antiserum, no preservative) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide from the phospho Y140/S156 of human Cofilin-1 (Cofilin, non-muscle isoform) |
Cofilin 1 |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(lyophilized, whole antiserum, no preservative) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide from the internal part of human Cofilin-1 (Cofilin, non-muscle isoform) |
Cofilin 1 |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(lyophilized, no preservative) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide from the internal part of human Cofilin-1 (Cofilin, non-muscle isoform) |
Cofilin 1 |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(lyophilized, no preservative, purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide from the phospho Y140/S156 of human Cofilin-1 (Cofilin, non-muscle isoform) |
Cofilin 1 |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(lyophilized, no preservative, purified) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide from the phospho Y140/S156 of human Cofilin-1 (Cofilin, non-muscle isoform) |
Cofilin 1 |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, PBS, no preservative, affinity purified) |
IH |
Human, Mouse, Rat |
- |
detects endogenous levels of Cofilin 1 protein |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, PBS, no preservative, affinity purified) |
IH |
Human, Mouse, Rat |
- |
detects endogenous levels of p-COFILN1 protein |
- |
|
|
Novus Biologicals |
kit
|
Unknown Host
(dual abs: 1) rabbit polyclonal phospho-CFLI Y88 and 2) mouse monoclonal CFL1) |
- |
Human |
- |
reacts with cofilin 1 (non-muscle) |
- |
|
|
Novus Biologicals |
kit
|
Unknown Host
(dual abs: 1) rabbit polyclonal phospho-CFLI Y139 and 2) mouse monoclonal CFL1) |
- |
Human |
- |
reacts with cofilin 1 (non-muscle) |
- |
|
|
Novus Biologicals |
monoclonal
IgG1, κ |
Mouse
(liquid, PBS, azide, affinity purified) |
ELISA, WB |
Human |
immunogen = recombinant human Cofilin 1 protein |
Cofilin 1 (non-muscle) |
- |
|
|
Novus Biologicals |
monoclonal
IgG2a, κ |
Mouse
(liquid, PBS, no preservative) |
ELISA, IF, IH, WB |
Human |
immunogen = CFL1 (AAH11005, aa1-167) full length recombinant protein with GST tag |
Cofilin 1 (non-muscle) |
- |
|
|
Novus Biologicals |
kit
|
Unknown Host
(dual abs: 1) rabbit polyclonal phospho-CFLI S3 and 2) mouse monoclonal CFL1) |
- |
Human |
- |
reacts with cofilin 1 (non-muscle) |
- |