|
|
Roche Applied Science |
polyclonal
|
Sheep
(IgG waspurified and and specificIgG was isolated by immunosorption. Fab fragments wereobtained by papain digestion, purified, PBS, BSA, lyophilized) |
IF, IH |
- |
immunogen = digoxigenin |
used for the detection of digoxigenin- labeled compounds. |
- |
|
|
Sigma Aldrich |
monoclonal
IgG1 |
Mouse
(purified Ig, buffered aqueous glycerol solution, PBS, BSA, preservative, Cy3™) |
IF |
Various species, Mouse, Rat |
- |
Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent; useful in detection of FLAG fusion proteins expressed in murine host |
- |
|
|
Sigma Aldrich |
monoclonal
IgG1 |
Mouse
(High Sensitivity, M2 coated 96-well plates) |
ELISA |
Various species |
- |
Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Suitable for screening for expression, study of protein:protein interactions and ELISA assays |
- |
|
|
Invitrogen Corporation |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide) |
ELISA, IH, IP, WB |
- |
immunogen = the c-myc epitope (recognizes the sequence:-Glu -Gln -Lys -Leu -Ile -Ser -Glu -Glu -Asp -Leu- ) |
for detection of recomb. proteins containing the c-myc epitope |
- |
|
|
Sigma Aldrich |
polyclonal
|
Rabbit
(agarose affinity gel, PBS, azide) |
IP |
- |
immunogen = synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein (Glu-Gln-Lys-Leu-Ile- Ser-Glu-Glu-Asp-Leu) |
N-terminal or C-terminal c-Myc tagged fusion proteins |
- |
|
|
Sigma Aldrich |
polyclonal
|
Rabbit
(buffered aqueous solution, PBS, BSA, azide) |
IB, IC, IP |
- |
- |
N-terminal or C-terminal c-Myc tagged fusion proteins |
- |
|
|
Sigma Aldrich |
polyclonal
|
Rabbit
(prepared by the two-step glutaraldehyde method; buffered aqueous solution, PBS, thimerosal, Peroxidase) |
IB |
- |
- |
N-terminal or C-terminal c-Myc tagged fusion proteins |
- |
|
|
Sigma Aldrich |
monoclonal
IgG1 |
Mouse
(liquid, purified, PBS suspension, azide, agarose; also available as purified Ig, H3663; glycerol, A5477;ascites, H9658;biotin, B9183;FITC, H7411;Peroxidase, H6533;TRITC, H9037) |
IP |
- |
immunogen = synthetic peptide corresp. to aa residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA), conjugated to KLH |
Reacts with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells |
- |
|
|
Sigma Aldrich |
monoclonal
IgG2a |
Mouse
(liquid, purified, PBS suspension, azide, agarose;) |
IP |
- |
immunogen = recombinant polyHistidine tagged fusion protein |
recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors |
- |
|
|
Bethyl Labs |
polyclonal
|
Rabbit
(affinity isolated, PBS, azide;also available as affinity purified and HRP-conj., A190-102P) |
ELISA, IC, WB |
- |
immunogen = ECS, Enterokinase Cleavage Site (xxxDDDDK) conjugated to KLH |
reacts with FLAGTM |
- |
|
|
Bethyl Labs |
polyclonal
|
Rabbit
(phosphate, saline, azide) |
ELISA, IC, WB |
- |
immunogen = ECS, Enterokinase Cleavage Site (xxxDDDDK) conjugated to KLH |
reacts with FLAGTM |
- |
|
|
Bethyl Labs |
polyclonal
|
Rabbit
(liquid, affinity purified, phosphate, saline, azide;also available HRP conj., A150-109P) |
ELISA, IC, IH, WB |
- |
- |
reacts specifically with KLH-biotin and BSAbiotin.There is no reaction with free KLH or BSA. |
- |
|
|
QIAGEN Inc. |
monoclonal
IgG |
Mouse
(liquid, stabilized stock solution, Biotin conj.) |
- |
- |
- |
detects five consecutive histidine residues. Negligible cross with crude E. coli, yeast, mammalian, or insect cell lysates |
- |
|
|
Calbiochem EMD Biosciences |
unknown
|
Unknown Host
(liquid slurry, PBS, azide, agarose conj., suspension, ready to use) |
- |
- |
- |
covalently coupled to the agarose support and does not leach. The protein A will bind IgG from biological fluids, but with different efficiencies, depending on the species. In general, Protein A works well for purification of IgG of large mammals, while Protein G PLUS and a mixture of Protein G PLUS with Protein A are best for rodent IgG purifications |
- |
|
|
Calbiochem EMD Biosciences |
unknown
|
Unknown Host
(liquid slurry, PBS, azide, agarose suspension, ready to use) |
IP |
- |
- |
designed for IP applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification |
- |
|
|
Calbiochem EMD Biosciences |
unknown
|
Unknown Host
(liquid slurry, PBS, azide, agarose conj., suspension, ready to use) |
- |
- |
- |
designed for purification of IgG from biological fluids The protein is covalently coupled to the agarose support and does not leach. The protein A will bind IgG from biological fluids, but with different efficiencies, depending on the species. In general, Protein A works well for purification of IgG of large mammals, while Protein G PLUS and a mixture of Protein G PLUS with Protein A are best for rodent IgG purifications |
- |
|
|
Calbiochem EMD Biosciences |
unknown
|
Unknown Host
(liquid slurry, PBS, azide, agarose conj., suspension, ready to use) |
IP |
- |
- |
has multiple Ig binding sites genetically engineered for higher binding capacity of mouse IgG1 or IgG3 as well as IgG from rabbit, rat, and other species. Mouse IgG2a or IgG2b bind moderately to Protein G PLUS |
- |
|
|
Invitrogen Corporation |
polyclonal
IgG |
Rabbit
(antiserum, affinity purified, PBS, azide,) |
ELISA, WB |
- |
Immunogen = recombi.t GST protein from Schistosoma japonicumPAD KG2 |
Specific for the ~26 kDa GST protein. Useful for detecting GST and GST-fusion proteins transcribed from pGEX and similar vectors |
- |
|
|
Roche Applied Science |
monoclonal
IgG1,κ |
Mouse
(purified by gel filtration, phosphate buffer, saline, MIT, lyophilized) |
ELISA, IB |
- |
immunogen = digoxigenin |
reacts with free and bound digoxigenin |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, ascites, no preservative) |
ELISA, IC, IP, WB |
Various vertebrate |
immunogen = Synthetic peptide (Cys)-DYKDDDK, coupled to ovalbumin |
Reacts with both N-terminal and C-terminal fusion proteins and does not require Ca++ for binding. Reacts better in most cases than M2 or M4 FLAG clones |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatinalso available as:sc-459 FITC,sc-459 B, sc-459 AF405,sc-459 AF459,sc-459 AF647) |
IF, IP, WB |
Human |
Immunogen = a sequence of GSTof Schistosoma japonicum origin |
recognizes 26 kDa glutathione S-transferase (GST) of Schistosoma japonicum origin and GST fusion proteins |
- |
|
|
MBL International |
polyclonal
IgG |
Rabbit
(liquid, PBS, glycerol, BSA, azide, purified) |
IP, WB |
Non-specific |
- |
reacts with wild-type GFP, and its variants EGFP and EBFP, etc. |
- |
|
|
Sigma Aldrich |
monoclonal
IgG2a |
Mouse
(liquid, ascites;also available asPeridoxase, A7058;Alkaline Phosphatase, A5588) |
DB, ELISA, IP, WB |
- |
immunogen = recombinant polyHistidine tagged fusion protein |
recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(gelatin, PBS, azide, agarose, conj) |
IF, IH, IP, WB |
Human, Mouse, Rat |
immunogen = peptide corresp. to aa 408-439 within the C-terminal domain of c-Myc of human origin |
detects c-Myc p67 |
- |
|
|
Upstate and Chemicon are Millipore |
monoclonal
IgG3 |
Mouse
(liquid, protein A purified, PBS, azide) |
IB, IC, IP |
- |
immunogen = KLH-conjugated, synthetic peptidecorresponding to amino acids 98-106 (YPYDVPDYA)of influenza hemaglutinin |
Recognizes HA tagged proteinsoverexpressed in cells, including both amino orcarboxy-termini of targeted proteins in transfectedmammalian cells |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1,κ |
Mouse
(liquid, ascites, azide, unpurified, no preservatives) |
ELISA, IC, IF, WB |
Non-specific |
immunogen = Cre recombinase (aa 77 to 343) fusion protein |
recognizes Cre recombinase, ~38 kDa |
- |
|
|
ATCC |
monoclonal
IgG1 |
Mouse
(frozen suspension) |
- |
Human |
immunogen = synthetic peptide derived using the sequence of the human c-myc gene product |
detects c-Myc |
- |
|
|
Roche Applied Science |
monoclonal
IgG1 |
Rat
(purified from bioreactor supernatants and lyophilized.Also available asBiotin, 12158167001Peroxidase, 12013819001) |
DB, ELISA, IC, IP, WB |
- |
immunogen = synthetic peptide (residues 76-111 of X47 hemaglutinin 1) coupled to keywhole limpet hemocyanin(KLH) |
for the detection of native and recombinant “epitope tagged” HA proteins; recognizes the HA peptide sequence (YPYDVPDYA) derived from the influenza hemagglutinin protein |
- |
|
|
Biolegend |
monoclonal
IgG1,κ |
Mouse
(affinity purified, PBS, azide) |
ELISA, IC, IF, IH, WB |
Non-specific |
immunogen = amino acids 408-439, C-terminal region of human c-myc |
reacts with human and fusion proteins in all species |
- |
|
|
Upstate and Chemicon are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, glycerol, Tris-glycine, saline, azide, protein G purified) |
EM, IC, IP, WB |
Non-specific |
immunogen = synthetic peptide corresponding to amino acids 408-437 of the Leucine zipper region of human Myc |
recognizes Myc and recombinant proteins containing the Myc epitope tag |
- |
|
|
Invitrogen Corporation |
monoclonal
IgG2a |
Mouse
(liquid, purified, PBS, HRP conj.) |
ELISA, IH, IP, WB |
- |
immunogen = the V5 epitope (recognizes the sequence:Gly-Lys-Pro-Ile- Pro- Asn-Pro-Leu-Leu-Gly- Leu-Asp-Ser-Thr- ) |
detects recombinant proteins containing the 14 amino acid V5 epitope. |
- |
|
|
Invitrogen Corporation |
monoclonal
IgG2a |
Mouse
(liquid, purified, PBS, azide) |
ELISA, IH, IP, WB |
- |
immunogen = the V5 epitope (recognizes the sequence:Gly-Lys-Pro-Ile- Pro- Asn-Pro-Leu-Leu-Gly- Leu-Asp-Ser-Thr- ) |
detects recombinant proteins containing the 14 amino acid V5 epitope. |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, Tris HCl, glycerol, no preservative, affinity purified) |
IC, IF, IH, IP, WB |
Non-specific |
- |
reacts with GFP |
- |
|
|
AbD Serotec |
monoclonal
IgG1 |
Rat
(liquid, affinity purified IgG, PBS, azide) |
IH, IP, WB |
Human |
immunogen = residues 408-439 corresp. to the C-terminal region of human c-myc |
recognises the myc epitope which is frequently used to tag proteins, and is the same epitope recognised by clone 9E10 |
- |
|
|
Roche Applied Science |
polyclonal
|
Sheep
(lyophilized. sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. phosphate buffer, saline, MIT) |
ELISA, IC, IH, WB |
- |
immunogen = digoxigenin |
100% reactivity with digoxigenin and digoxin, but no cross-reactivity with other steroids, such as human estrogens or androgens |
- |
|
|
Roche Applied Science |
polyclonal
|
Sheep
(stabilized solution. IgG waspurified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer) |
DB, ELISA, IC, IH, NB, SB, WB |
- |
immunogen = digoxigenin |
100% reactivity with digoxigenin and digoxin, but no cross-reactivity with other steroids, such as human estrogens or androgens |
- |
|
|
Santa Cruz |
monoclonal
IgG2a |
Rat
(liquid, PBS, azide, gelatinAlso available as phycoerythrin (sc-53516 PE) or fluorescein (sc-53516 FITC)conjugates for flow cytometry) |
FC, IF, IP, WB |
Various species |
Immunogen = HA peptide derived from influenza hemaglutinin |
for detection of proteins containing theHA tag |
- |
|
|
Santa Cruz |
monoclonal
IgG2a |
Mouse
(liquid, PBS, azide, gelatin.also available as: sc-7392 AC;sc-7392 B;sc-7392 PE;sc-7392 HRP;sc-7392 FITC;sc-7392 TRITC;Alexa Fluor®s and TransCruz reagent for ChIP, sc-7392 X) |
ELISA, IF, IP, WB |
Various species |
Immunogen = an internal region of the influenza hemaglutinin (HA) protein |
for detection of proteins containing theHA tag |
- |
|
|
Santa Cruz |
monoclonal
IgG3 |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
Various species |
Immunogen = a synthetic peptide derived from the human influenza hemaglutinin |
for detection of proteins containing theHA tag |
- |
|
|
Santa Cruz |
monoclonal
IgG3 |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
Various species |
Immunogen = synthetic peptide derived from influenza hemaglutinin |
for detection of proteins containing theHA tag |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(liquid, antisera, phosphate, saline, azide, affinity purifued) |
ELISA, WB |
Human, Mouse, Rat |
immunogen = keyhole limpet haemocyanin conjugated Enterokinase cleavage site peptide DYKDDDDK |
specific for the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp- Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(whole antisera, phosphate, saline, azide) |
EM, IC, IH, IP, WB |
- |
immunogen = highly purified recombinant full length protein made in E. coli. The antibody is directed against the entire GFP molecule |
reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(whole antisera, phosphate, saline, azide) |
IC, IF, IH, IP, WB |
- |
immunogen = GFP fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria |
reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Goat
(liquid, antisera, affinity purified, PBS, azide,) |
ELISA, IC, IP, WB |
- |
Immunogen = GST protein from Schistosoma japonicum |
recognizes Glutathione-S-Transferase (GST) |
- |
|
|
Novus Biologicals |
monoclonal
IgG1 |
Mouse
(protein-G purified ascites, PBS, azide, Biotin conj.) |
ELISA, WB |
- |
immunogen = PAX6 transcription factor linked to histidine tag |
recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags; against all His tagged proteins so far tested |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(liquid, antisera, PBS, Pro-Clean 400, HRP conj, affinity purified) |
ELISA, IC, IH, WB |
- |
Immunogen = Goat IgG whole molecule |
- |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(affinity purified, PBS, azide) |
ELISA, IC, IP, WB |
Non-specific |
immunogen = synthetic peptide representing amino acid residues 95 to 108 of RNA polymerase alpha subunit of simion virus 5 conjugated to KLH |
recognizes the V5 cleavage site |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(affinity purified, PBS, azide) |
ELISA, IC, IH, WB |
Non-specific |
immunogen = c-myc (EQKLISEEDL) conjugated to KLH |
recognizes c-myc |
- |
|
|
Sigma Aldrich |
monoclonal
IgG1 |
Mouse
(liquid, purified IgG, PBS, BSA, azide) |
ELISA, IB, IC, IP |
- |
immunogen = synthetic peptide corresp. to aa residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA), conjugated to KLH |
Reacts with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells |
- |
|
|
Sigma Aldrich |
monoclonal
IgG1 |
Mouse
(ascites fluid, azide) |
ELISA, IB, IC, IP |
- |
immunogen = synthetic peptide corresp. to aa residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA), conjugated to KLH |
Reacts with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells |
- |
|
|
Novus Biologicals |
monoclonal
IgG1, κ |
Mouse
(liquid, ascites, glycerol, BSA, PBS, Thimerosal, purified, conj. biotin) |
ELISA, IH, SB, WB |
Non-specific |
- |
reacts with free and bound digoxigenin |
- |
|
|
Novus Biologicals |
monoclonal
IgG1, κ |
Mouse
(liquid, ascites, no preservative) |
ELISA, IH, WB |
Non-specific |
- |
reacts with free and bound digoxigenin |
- |
|
|
Novus Biologicals |
monoclonal
IgG1, κ |
Mouse
(liquid, purified ascites, PBS, BSA, glycerol, thimerosal, conj. HRP) |
ELISA, IH, WB |
Non-specific |
- |
reacts with free and bound digoxigenin |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Sheep
(liquid, antiserum, PBS, azide, protein G purified) |
ELISA, IH, WB |
- |
immunogen = digoxin-3-CMO conjugated to KLH |
digoxigenin; cross reacts with digoxin and digitoxin; negligible reactivity has been observed with Ouabain |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, ascites, BSA, glycerol, PBS, no preservative) |
ELISA, IH, SB, WB |
Non-specific |
- |
reacts with free and bound digoxigenin |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, BSA, glycerol, PBS, thimerosal, conj. biotin) |
ELISA, IH, SB, WB |
Non-specific |
- |
reacts with free and bound digoxigenin |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, purified ascites, PBS, BSA, glycerol, thimerosal, conj. HRP) |
ELISA, IH, SB, WB |
Non-specific |
- |
reacts with free and bound digoxigenin |
- |
|
|
Abcam |
polyclonal
IgG |
Sheep
(liquid, antiserum, PBS, azide, protein G purified) |
ELISA, IH, WB |
- |
immunogen = digoxin-3-CMO conjugated to KLH |
Digoxigenin |
- |
|
|
Abcam |
polyclonal
IgG |
Goat
(liquid, PBS, azide, ion exchange chromatography) |
ELISA, WB |
- |
immunogen = digoxigenin conj. to KLH |
Digoxigenin and digoxin; no cross-reaction with the carrier protein KLH or other steroids |
- |
|
|
Abcam |
polyclonal
IgY |
Chicken
(liquid, BSA, thimerosal, Hepes-saline, affinity purified, conj. HRP) |
ELISA, WB |
- |
immunogen = digoxigenin conj. to KLH |
Digoxigenin |
- |
|
|
Abcam |
polyclonal
IgY |
Chicken
(liquid, PBS, azide, affinity purified) |
WB |
- |
immunogen = digoxigenin conj. to KLH |
Digoxigenin |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
IF, IH, WB |
Non-specific |
immunogen = Digoxigenin |
reacts with free and bound digoxigenin |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
IF, IH, WB |
Non-specific |
immunogen = Digoxigenin |
reacts with free and bound digoxigenin |
- |
|
|
LifeSpan Biosciences, Inc. |
polyclonal
IgG |
Sheep
(liquid, BSA, PBS, azide, affinity purified, modified Biomagnetic particle (BMP)) |
RIA |
- |
immunogen = Digoxigenin |
Digoxigenin |
- |
|
|
Santa Cruz |
monoclonal
IgG2b |
Mouse
(liquid, affinity purified,) |
ELISA, IF, IH, WB |
- |
immunogen = full length biotin |
permits the formation of antibody-Biotin complexes, thus enhancing the sensitivity of the detection system; non cross-reactive with the free carrier protein |
- |
|
|
Santa Cruz |
monoclonal
IgG2a |
Mouse
(liquid, affinity purified,) |
WB |
- |
immunogen = biotinylated denatured Ovalbumin |
permits the formation of antibody-Biotin complexes, thus enhancing the sensitivity of the detection system |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, affinity purified,) |
WB |
- |
immunogen = biotin |
detection of both free and carrier protein bound biotin; also recommended for detection of biocytin |
- |
|
|
Santa Cruz |
monoclonal
IgG2b |
Mouse
(liquid, ascites, azide, affinity purified) |
ELISA, IF, IH, WB |
- |
immunogen = full length biotin |
permits the formation of antibody-Biotin complexes, thus enhancing the sensitivity of the detection system; non cross-reactive with the free carrier protein |
- |
|
|
Santa Cruz |
monoclonal
IgG2a |
Mouse
(liquid, PBS, gelatin, azide) |
WB |
- |
immunogen = biotinylated denatured Ovalbumin |
permits the formation of antibody-Biotin complexes, thus enhancing the sensitivity of the detection system |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, affinity purified) |
IF, IP, WB |
- |
immunogen = biotin conj. KLH |
detection of biotin; permits the formation of antibody-Biotin complexes, thus enhancing the sensitivity of the detection system |
- |
|
|
Santa Cruz |
monoclonal
IgG3 |
Mouse
(liquid, ascites, azide, BSA) |
- |
- |
immunogen = biotin labeled DNA and biotin-labeled alkaline phosphatase |
detection of biotin; permits the formation of antibody-Biotin complexes, thus enhancing the sensitivity of the detection system; non cross-reactive with Biotin, Digoxigenin, or BSA |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(IgG, PBS, gelatin, azide;agarose conjugate for IP, sc-8334 AC,HRP conjugate for WB, sc-8334 HRP,biotin conjugate, sc-8334 B) |
ELISA, IF, IP, WB |
Non-specific |
immunogen = peptide of amino acids 1-238representing full length GFP of Aequorea victoria origin |
detection of GFP |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin.also available as: sc-805 AC;sc-805 HRP;;sc-805 FITC;sc-805 TRITC;biotin: sc-805 B;Alexa Fluor®ssc-805 AF405,sc-805AF488,sc-805 AF647and TransCruz reagent for ChIP, sc-805 X) |
IF, IP, WB |
Non-specific |
Immunogen = a peptide mapping within aninternal region of the influenza hemagglutinin (HA) protein |
for detection of proteins containing theHA tag |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1κ |
Mouse
(liquid, protein-G purified mouse immunoglobulin, PBS, azide) |
WB |
Various vertebrate |
immunogen = full length GFP protein |
reacts with the 28 kDa GFP protein |
- |
|
|
AnaSpec |
monoclonal
IgG1,κ |
Mouse
(liquid, affintiy purified, PBS, BSA, azide) |
FC, WB |
Non-specific |
Immunogen = asynthetic peptides with biotin conjugated to BSA |
recognizes Bag-1 |
- |
|
|
Cell Signaling |
monoclonal
IgG1 |
Mouse
(liquid, sodium HEPES, saline, BSA, glycerol, azideAvailable as Alexa Fluor® 488 Conjugate, #2350 and as Alexa Fluor® 647 Conjugate, #3444) |
FC, IC, IF, IH, WB |
Non-specific |
immunogen = synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA) |
detects recombinant proteins containing the HA epitope tag; recognizes the HA-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells |
- |
|
|
Sigma Aldrich |
monoclonal
IgG1 |
Mouse
(purified Ig, buffered aqueous glycerol solution, TBS, glycerol, stabilizer, preservative, AP conj.also available as HRP conj., A8592 or biotin labeled, F9291) |
DB, ELISA, WB |
Various species, Mouse, Rat |
- |
Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent; useful in detection of FLAG fusion proteins expressed in murine host |
- |
|
|
AnaSpec |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, PBS, azide) |
WB |
Aequorea victoria |
immunogen = a synthetic peptide corresponding to an internal sequence of green-fluorescent protein (GFP) isolated from Aequorea victoria |
detects GFP |
- |
|
|
AnaSpec |
polyclonal
IgY |
Chicken
(liquid, affinity purified, PBS, azide) |
ELISA, IH, IP, WB |
Aequorea victoria |
immunogen = full length GFP highly purified from Aequorea victoria |
detects GFP |
- |
|
|
AnaSpec |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, PBS, azide) |
IH, IP, WB |
Aequorea victoria |
immunogen = highly purified green-fluorescent protein (GFP) isolated directly from Aequorea victoria |
detects GFP |
- |
|
|
AnaSpec |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, PBS, azide) |
WB |
Aequorea victoria |
immunogen = a synthetic peptide corresponding to the C-terminus of green-fluorescent protein (GFP) isolated from Aequorea victoria |
detects GFP |
- |
|
|
AnaSpec |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, PBS, azide) |
WB |
Aequorea victoria |
immunogen = a synthetic peptide corresponding to the N-terminus of green-fluorescent protein (GFP) isolated from Aequorea victoria |
detects GFP |
- |
|
|
Sigma Aldrich |
monoclonal
IgG1 |
Rabbit
(ascites fluid, azide) |
ELISA, IB, IF |
Various species |
immunogen = the FLAG™ peptide sequenceconjugated to KLH |
reacts with N-terminaland C-terminal FLAG fusion proteins. |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, purified, PBS, azide, gelatin.also available as: sc-803 AC for IP;sc-803 FITC for IF) |
IF, IP, WB |
Non-specific |
Immunogen = a peptide mapping to the polyhistidine domains of pET and Xpress polyhistidine expression vectors |
for detection of fusion proteins encoded by prokaryotic pET, pRSET A, B, C and pTrc His A, B, C; baculovirus pBlue Bac His A, B, C and mammalian pEBV His A, B, C expression vectors |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, PBS, azide, gelatin) |
ELISA, IP, WB |
Non-specific |
Immunogen = a peptide mapping ofHis-probe |
for detection of fusion proteins encoded by pET expression vectors with polyhistidine sequence domains |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
WB |
Non-specific |
Immunogen = a His-tagged recombinant protein |
for detection of fusion proteins encoded by polyhistidine expression vectors |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatinAvailable as biotin conjugate, sc-8036 B;as sc-8036 HRP, for WB;as sc-8036 AC, for IP) |
ELISA, IP, WB |
Non-specific |
Immunogen = a His-tagged recombinant protein |
for detection of fusion proteins encoded by polyhistidine expression vectors |
- |
|
|
Santa Cruz |
monoclonal
IgG2b |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
Non-specific |
Immunogen = a 6x His-tagged polypeptide |
for detection of fusion proteins encoded by polyhistidine expression vectors |
- |
|
|
Santa Cruz |
monoclonal
IgG2b |
Mouse
(liquid, PBS, azide, gelatin) |
WB |
Non-specific |
Immunogen = a 6x His-tagged homooligopeptide |
for detection of linear HHH HHH origin |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
IF, WB |
Non-specific |
Immunogen = a 6x His-tagged homooligopeptide |
for detection of linear HHH HHH origin |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
IF, IP, WB |
Non-specific |
Immunogen = a polypeptide containing a 6X polyhistidine tag |
for detection of fusion proteins encoded by polyhistidine expression vectors |
- |
|
|
AnaSpec |
monoclonal
IgG2a |
Mouse
(liquid, PBS, BSA, azide) |
IH, IP, WB |
Non-specific |
immunogen = a synthetic GST peptide |
detects GST |
- |
|
|
AnaSpec |
monoclonal
IgG2a |
Mouse
(liquid, PBS, BSA, azide) |
IH |
Non-specific |
immunogen = a synthetic GST peptide |
detects GST |
- |
|
|
AnaSpec |
monoclonal
IgG2a |
Mouse
(liquid, PBS, BSA, azide) |
IH |
Non-specific |
immunogen = a synthetic GST peptide |
detects GST |
- |
|
|
AnaSpec |
monoclonal
IgG2a |
Mouse
(liquid, PBS, BSA, azide) |
IH, IP, WB |
Non-specific |
immunogen = a synthetic GST peptide |
detects GST |
- |
|
|
AnaSpec |
monoclonal
IgG2a |
Mouse
(liquid, PBS, BSA, azide) |
IH, IP, WB |
Non-specific |
immunogen = a synthetic GST peptide |
detects GST |
- |
|
|
AnaSpec |
monoclonal
IgG2a |
Mouse
(liquid, PBS, BSA, azide) |
IH, IP, WB |
Non-specific |
immunogen = a synthetic GST peptide |
detects GST |
- |
|
|
AnaSpec |
monoclonal
IgG2a |
Mouse
(liquid, PBS, BSA, azide) |
IH, IP, WB |
Non-specific |
immunogen = a synthetic GST peptide |
detects GST |
- |
|
|
AnaSpec |
monoclonal
IgG2a |
Mouse
(liquid, PBS, BSA, azide) |
IH, IP, WB |
Non-specific |
immunogen = a synthetic GST peptide |
detects GST |
- |