Cyclin B1
LifeSpan Biosciences, Inc.
polyclonal
Rabbit
(DEAE-column chromatography)
-
Human, Mouse, Rat
immunogen = cyclin B1
cyclin B1
-
Cyclin B1
LifeSpan Biosciences, Inc.
polyclonal
Rabbit
(protein A purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to aa 420-433 (Cys-S A L V Q D L A K A V A K V) from the C-terminus of human cyclin B1, coupled to KLH
cyclin B1
-
Cyclin B1
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(protein G purified)
-
Human, Hamster, Mouse, Rat
immunogen = hamster cyclin B1 protein
Cyclin B1 (62kDa)
-
Cyclin B1
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(protein G purified)
-
Human, Hamster, Mouse, Rat
immunogen = hamster cyclin B1 protein
Cyclin B1 (62kDa)
-
Cyclin B1
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(protein A purified)
-
Human, Hamster, Mouse, Rat
immunogen = human cyclin B1 protein
Cyclin B1 (62kDa)
-
Cyclin B1
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(protein A purified)
-
Human, Hamster, Mouse, Rat
immunogen = human cyclin B1 protein
Cyclin B1 (62kDa)
-
Cyclin B1
LifeSpan Biosciences, Inc.
polyclonal
Rabbit
(unpurified serum)
-
Various species, Human, Mouse, Rat
immunogen = full length GST fusion protein corresponding to the human gene
cyclin B1; no reaction with cyclin B2 in WB
-
Cyclin B1
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(protein G purified)
-
Human, Mouse
immunogen = recombinant human cyclin B1 protein
cyclin B1 (58kDa); no cross reaction with cyclin A or cyclin D
-
Cyclin B1
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(protein G purified)
-
Human, Hamster, Mouse, Rat
immunogen = hamster cyclin B1 protein
cyclin B1 (58kDa); no cross reaction with cyclin A or cyclin D
-
Cdk1 (A17.1.1)
Chemicon and Upstate are Millipore
monoclonal
IgG2a
Mouse
(liquid, PBS, BSA, azide, purified)
-
Chicken, Human, Mouse, Rat, Xenopus
immunogen = Xenopus p34cdc2
recognizes cdk1 (34 kDa) (cdk1/p34cdc2 protein kinase, a catalytic subunit of maturation promoting factor); epitope maps near the C-terminus of protein and its core is thought to be L G T P N N E V; shows no cross-reaction with cdk2/p32 and other cyclin dependent kinases (cdks)
-
Cdc2
Epitomics
monoclonal
IgG
Rabbit
(liquid Tris-glycine, glycerol, azide)
-
Various species, Human
Immunogen = synthetic peptide corresponding to resiudes of human Cdc2
Cdc2
-
cdc2 phos Tyr15
Chemicon and Upstate are Millipore
polyclonal
Ig
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified)
-
Rat
immunogen = synthetic peptide corresp. to aa surrounding the phospho Try15 of human, rat, mouse and Xenopus CDC2
CDC2, phosphoTyr15 ~35kDa (labeling of CDC2 in WB of rat brain is blocked by the phosphopeptide but not by the dephosphopeptide
-
cdc2 phos Tyr15
Chemicon and Upstate are Millipore
polyclonal
Ig
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified)
-
Rat
immunogen = synthetic peptide corresp. to aa surrounding the phosphoTry15 of human, rat, mouse and Xenopus CDC2
CDC2, phosphoTyr15 ~35kDa (labeling of CDC2 in WB of rat brain is blocked by the phosphopeptide but not by the dephosphopeptide
-
Cdk4 (DCS-35)
Chemicon and Upstate are Millipore
monoclonal
IgG1 ,κ
Mouse
(liquid, PBS, BSA, azide, purified)
-
Pig, Human, Mouse, Rat
immunogen = purified recombinant cdk4 protein.
p34 or cyclin-dependent kinase 4 (cdk4); no cross-reaction with other cdks
-
Cyclin B1 phos Ser147
LifeSpan Biosciences, Inc.
polyclonal
Rabbit
(protein A purified)
-
Human, Mouse, Rat
immunogen = synthetic phospho-Ser147 peptide corresp. to residues surrounding serine 147 of human cyclin B1 (KLH coupled)
detects endogenous cyclin B1 only when phosphorylated on serine147
-
Cyclin B1
LifeSpan Biosciences, Inc.
polyclonal
Rabbit
(protein A purified)
-
Human
immunogen = peptide corresponding to aa 420-433 (Cys-S A L V Q D L A K A V A K V) from the C-terminal of human cyclin B1, conj. to KLH
cyclin B1
-
Cyclin B1 (V152)
Chemicon and Upstate are Millipore
monoclonal
IgG1
Mouse
(liquid, ascites, no preservative)
-
Human, Mouse
immunogen = hamster Cyclin B1
Cyclin B1
-
Cyclin B1
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(liquid, serum, azide, delipidized, defibrinated)
-
Human, Mouse, Rat
immunogen = full length human Cyclin B1 fused to GST
Cyclin B1; no cross-reactivity with Cyclin B2 in WB
-
Ki-67 (Ki-S5)
Chemicon and Upstate are Millipore
monoclonal
IgG1
Mouse
(liquid, purified, phosfate buffer, azide)
-
Human
immunogen = nuclear protein preparation from human cell line U937.50
reacts with 345 kD and 395 kD Ki-67 nuclear antigen expressed on human proliferating cells
-
Ki-67
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(liquid, affinity purified Ig, PBS, BSA, azide)
-
Human, Rat
immunogen = synthetic peptide from human Ki67 protein
Ki67
-
Ki-67
LifeSpan Biosciences, Inc.
monoclonal
Mouse
-
Human
immunogen = nuclear protein preparation from human cell line U937.50
Ki-67
-
Ki-67
LifeSpan Biosciences, Inc.
monoclonal
Rabbit
(supernatant)
-
Dog, Porcine, Human, Mouse, Rat
immunogen = synthetic peptide derived from the human Ki-67 protein; epitope = C- terminus
Ki-67
-
Ki-67
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(protein A purified, FITC conj.)
-
Human
immunogen = synthetic peptide corresponding to a carboxy terminal portion of human Ki-67 protein
Ki-67
-
Ki-67
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(supernatant)
-
Human
immunogen = human tumor cell line
Ki-67
-
Ki-67
LifeSpan Biosciences, Inc.
polyclonal
Rabbit
(DEAE-column chromatography)
-
Human, Rat
immunogen = synthetic peptide from C-terminus of human Ki67
Ki-67
-
Ki-67
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(DEAE-column chromatography)
-
Human
immunogen = Ki-67 synthetic peptide
Ki-67
-
Ki-67
LifeSpan Biosciences, Inc.
polyclonal
Rabbit
(protein A purified)
-
Human, Rat
immunogen = synthetic peptide from the human Ki67 protein. epitope: middle of Ki67 protein
Ki-67
-
Ki-67
LifeSpan Biosciences, Inc.
polyclonal
Rabbit
(protein A purified)
-
Human, Rat
immunogen = synthetic peptide from the human Ki67 protein
Ki-67
-
Ki-67
LifeSpan Biosciences, Inc.
polyclonal
Rabbit
(DEAE-column chromatography)
-
Human
immunogen = synthetic peptide corresp. to a C-terminal segment of human Ki-67
Ki-67
-
Cyclin B1 (V152)
Cell Signaling
monoclonal
IgG1
Mouse
(HEPES, NaCl, BSA, azide, conj. to Alexa Fluor® 488)
-
Human
immunogen = peptide corresp. to a seq. from hamster cyclin B1
detects endogenous levels of cyclin B1 (60kDa) independent of phosphorylation
-
Cyclin B1 (V152)
Cell Signaling
monoclonal
IgG1
Mouse
(HEPES, NaCl, BSA, azide, conj. to Alexa Fluor® 647)
-
Human
immunogen = peptide corresp. to a seq. from hamster cyclin B1
detects endogenous levels of cyclin B1 (60kDa) independent of phosphorylation
-
Cyclin B1 (V152)
Cell Signaling
monoclonal
IgG1
Mouse
(HEPES, NaCl, BSA, azide, conj. to Alexa Fluor® 488)
-
Human
immunogen = peptide corresp. to a seq. from hamster cyclin B1
detects endogenous levels of cyclin B1 (60kDa) independent of phosphorylation
-
Cyclin B1
Cell Signaling
polyclonal
Rabbit
(HEPES, NaCl, BSA, glycerol, affinity purified)
-
Human, Monkey, Hamster, Mouse, Rat
immunogen = syntheic peptide corresp. to residues near the N- terminus of human cyclin B1, (KLH-coupled)
detects total levels of cyclin B1 (60kDa)
-
Cyclin B1[O.N.238]
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
immunogen = recombinant protein corresp. to human cyclin B1
recognizes 60kDa cyclin B1
-
Cyclin B1[H-20]
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human
immunogen = C-terminus of human cyclin B1
recognizes 60kDa cyclin B1
-
Cyclin B1[M-20]
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
immunogen = C-terminus of rat cyclin B1
recognizes 60kDa cyclin B1
-
Cyclin B1[V143.1]
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Hamster, Mouse
immunogen = hamster His-tagged cyclin B1 expressed by bacteria
recognizes 60kDa cyclin B1
-
Cyclin B1[V92.1]
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Human, Hamster, Mouse
immunogen = hamster cyclin B1
recognizes 60kDa cyclin B1
-
Cyclin B1[V63]
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Human, Hamster, Mouse
immunogen = hamster cyclin B1
recognizes 60kDa cyclin B1
-
Cyclin B2[2115C1]
Santa Cruz
monoclonal
IgG1
Mouse
-
Human
immunogen = recombinant protein corresp. to the N-terminal region of human cyclin B2
cyclin B2
-
Cyclin B2[A-2]
Santa Cruz
monoclonal
IgG1
Mouse
-
Human, Mouse, Rat
immunogen = aa 1-105 of human cyclin B2
cyclin B2
-
Cyclin B2[C-13]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide)
-
Human
immunogen = peptide mapping at the C-terminus of human cyclin B2
cyclin B2
-
Cyclin B2[H-105]
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
epitope = corresp. to aa 1-105 of cyclin B2
cyclin B2
-
Cyclin B2[K-16]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide)
-
Mammal (various), Human, Mouse, Rat
immunogen = peptide mapping at internal region of mouse cyclin B2
cyclin B2
-
Cyclin B2[N-20]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide)
-
Mammal (various), Human, Mouse, Rat
epitope = mapping at N-terminus of human cyclin B2
cyclin B2
-
Cyclin B[dC-20]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Drosophila
epitope = mapping within an internal region of drosophila melanogaster cyclin B
cyclin B2
-
Cyclin B[dN-17]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Drosophila
epitope = mapping at N-terminus of drosophila melanogaster cyclin B
cyclin B
-
Cyclin B[d-300]
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Drosophila
epitope = corresp. to aa 1-300 of drosophila melanogaster cyclin B
cyclin B
-
Cyclin B2[X29.2]
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat, Xenopus
immunogen = Xenopus cyclin B2
cyclin B2
-
Cyclin B2[X121.10]
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Xenopus
immunogen = Xenopus cyclin B2
cyclin B2
-
Cyclin B-like[aQ-15]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Arabidopsis thaliana
epitope = mapping near the C-terminus of Arabidopsis thaliana B-like cyclin
B-like cyclin
-
Cyclin B-like[aC-15]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Arabidopsis thaliana
epitope = mapping near the N-terminus of Arabidopsis thaliana B-like cyclin
B-like cyclin
-
Cyclin B1[D-11]
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Human
immunogen = full length cyclin B1 of human origin (aa 1-433)
recognizes 60kDa cyclin B1
-
Cyclin B1[GNS1]
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
immunogen = recombinant protein corresp. to human cyclin B1
recognizes 60kDa cyclin B1
-
Cyclin B1[V152]
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Human, Hamster, Mouse
immunogen = hamster His-tagged cyclin B1 expressed by bacteria
recognizes 60kDa cyclin B1
-
Ki-67 (SP6)
Lab Vision Thermo Scientific
monoclonal
IgG
Rabbit
(tissue culture supernatant with azide, orready-to-use for IHalso available FITC conj.)
-
Dog, Swine, Human, Mouse, Rat
immunogen = synthetic peptide derived from the human Ki-67 protein (epitope in C-terminus)
Ki67 nuclear protein, expressed in proliferating cells; preferentially expressed during late G1-, S-, M-, and G2-phases
-
Ki-67 (Ki-S5)
Lab Vision Thermo Scientific
monoclonal
IgG1 ,κ
Mouse
(supernatant, azide or predilute for IH(P))
-
Human
immunogen = nuclear preparation from U937cell line (epitope Formalin resistant)
Ki67 nuclear protein, expressed in proliferating cells
-
Ki-67
Lab Vision Thermo Scientific
polyclonal
IgG
Rabbit
(Ig fraction, affinity purified, PBS, BSA, azide)
-
Human, Rat
immunogen = 345kDa synthetic peptide from C-terminus of human Ki67
Ki67 nuclear protein, expressed in proliferating cells
-
Ki-67
Lab Vision Thermo Scientific
polyclonal
IgG
Rabbit
(serum IgG, protein-A purified, PBS, BSA, azide or predilute for IH(P))
-
Human, Rat
immunogen = synthetic peptide from middle of human Ki67 protein
Ki67 nuclear protein, expressed in proliferating cells
-
p21 [187]
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Human
immunogen = full length human p21
p21
-
p21 [AC8.3]
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Human
immunogen = His-tagged human p21 expressed in baculovirus
p21
-
p21 [DCS-60]
Santa Cruz
monoclonal
IgG2a
Mouse
(liquid, PBS, gelatin, azide)
-
Human
immunogen = full length human p21
p21; no cross with other mitotic inhibitors
-
p21 [O.N.488]
Santa Cruz
monoclonal
IgG2a
Mouse
(liquid, PBS, gelatin, azide)
-
Human
immunogen = full length human p21
p21; no cross with other mitotic inhibitors
-
p21 [DF10]
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Human
immunogen = aa 58-77 of human p21 WAF 1
p21
-
p21 [3G223]
Santa Cruz
monoclonal
IgG2a
Mouse
(liquid, PBS, gelatin, azide)
-
Human
immunogen = aa 58-77 of human p21 WAF 1
p21
-
p21 (H-164)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
immunogen = epitope corresp. to aa 1-164 representing full length p21 of human origin
p21; partially cross-reactive with the related mitotic inhibitory protein, p27
-
p21 (M-19)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Mammal (various), Cat, Human, Mouse, Rat
immunogen = epitope mapping at the C-terminus of mouse p21
p21; partially cross-reactive with the related mitotic inhibitory protein, p27
-
p21 (N-20)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide, affinity purified)
-
Human
immunogen = epitope mapping at the N-terminus of mouse p21
p21
-
p21 [SPM306]
Santa Cruz
monoclonal
IgG2a
Mouse
(liquid, PBS, gelatin, azide)
-
Human
immunogen = recombinant full length human p21
p21
-
p21 [SX118]
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Human, Mouse
immunogen = aa 146-164 of human p21
p21
-
p21 [WA-1]
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Human
immunogen = aa 146-164 of human p21
p21
-
p21 phos Ser146
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = short aa seq. containing phosphorylated Ser 146 of human p21
p21 phosphorylated Ser146
-
p21 phos Thr145
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
immunogen = short aa seq. containing phosphorylated Thr 145 of human p21
p21 phosphorylated Thr145
-
p21 (F-5)
Santa Cruz
monoclonal
IgG2b
Mouse
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
immunogen = aa 1-159 representing full length mouse p21
p21
-
p19ARF [5-C3-1]
Santa Cruz
monoclonal
IgG2b
Rat
(liquid, PBS, azide, gelatin)
-
Mouse
immunogen = mixture of two peptides corresp. to aa 54-75 and 156-169 of mouse p19 ARF; epitope mapping to aa 62-75
detects p19ARF
-
p19ARF [12-A1-1]
Santa Cruz
monoclonal
IgG2b
Rat
(liquid, PBS, azide, gelatin)
-
Mouse
immunogen = mixture of two peptides corresp. to aa 54-75 and 156-169 of mouse p19 ARF; epitope mapping to aa 54-62
detects p19ARF
-
p19ARF [G-19]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
-
Mouse, Rat
immunogen = epitope mapping at the N-terminus of mouse p19 ARF
detects p19ARF
-
p19ARF [M-60]
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Mouse
immunogen = epitope corresp. to aa 6-65 mapping near the N-terminus of mouse p19 ARF
detects p19ARF
-
p27Kip1 (G173-524)
BD Pharmingen
monoclonal
IgG1
Mouse
(aqueous, buffered, azide)
-
Human, Mouse
immunogen = full length mouse p27 recomb. protein
identifies p27
-
APC (Ab-5) (CF11)
Calbiochem EMD Biosciences
monoclonal
IgG2b
Mouse
(liquid, phosphate buffer, gelatin, azide)
-
Human, Mouse
immunogen = recomb. protein consisting of the N-terminal 226 aa of APC
various truncated and full lenghth forms of the APC protein have been observed in tumor cell lines and in lymphocytes
-
APC (Ab-7) (CC-1)
Calbiochem EMD Biosciences
monoclonal
IgG2b
Mouse
(liquid, phosphate buffer, gelatin, azide)
-
Human, Mouse, Rat
immunogen = recomb. protein consisting of aa 1-226 of APC
recognizes the ~312 kDa APC protein; stains the cell body as opposed to the myelinated processes, so is well suited for IHC and IF studies of oligodendrocytes
-
APC (Ab-2) (IE1)
Calbiochem EMD Biosciences
monoclonal
IgG1
Mouse
(lyophilized from a volatile buffer, BSA)
-
Human
immunogen = recomb. protein consisting of the C-terminal 306 amino acids of human APC
Recognizes full length p300 APC in normal cells and in peripheral mononuclear cells; will not detect truncated APC protein
-
APC (Ab-1) (FE9)
Calbiochem EMD Biosciences
monoclonal
IgG1
Mouse
(liquid, sodium phosphate buffer, gelatin, azide)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresponding to the N-terminal 35 amino acids of APC
Recognizes the ~300 kDa APC protein as well as a variety of truncated forms
-
Cyclin B1
LifeSpan Biosciences, Inc.
monoclonal
Rabbit
(supernatant)
-
Human
immunogen = synthetic peptide corresp. to residues in C-terminus of human cyclin B1
Cyclin B1
-
Rac1 (C11)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
(epitope mapping within the C-terminus of Rac-1 of human origin)
recognizes Rac1 p21 and, to a lesser extent, Rac3; no cross with Rac2
-
Rac1 (C14)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, gelatin, azideavailable as agarose conj SC-217 AC)
-
Human, Mouse, Rat
(epitope mapping at the C-terminus of Rac-1 of human origin)
recognizes Rac1 p21 and, to a lesser extent, Rac3; no cross with Rac2
-
Rac1 (T17)
Santa Cruz
polyclonal
IgG
Goat
(liquid, affinity purified, PBS, gelatin, azide)
-
Dog, Cow, Human, Mouse, Rat
(epitope mapping near the C-terminus of Rac-1 of human origin)
recognizes Rac1 p21, Rac2 p21 and Rac3
-
Cdk1/cdc2 (DW3)
Upstate and Chemicon are Millipore
monoclonal
IgG
Rabbit
(liquid, tris-glycine saline, glycerol, azide, protein A purified)
-
Mammal (various), Human
immunogen =His-tagged, full-length recombinant human cdk1/cdc2
cdk1/cdc2 kinase
-
Cdk1/cdc2
Upstate and Chemicon are Millipore
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Mammal (various)
immunogen = purified synthetic peptide corresp. to the C-terminal region of human cdc2
cdk1/cdc2 kinase
-
Cdk1/cdc2
Upstate and Chemicon are Millipore
polyclonal
IgG
Rabbit
(liquid, serum, azide)
-
Human, Mouse, Rat
immunogen = synthetic peptide ( C F L S K M L V Y D P A K R I S G K M A L K H P Y F) corresp. to aa 263-287 of cdk1/cdc2, KLH-conjugated
cdk1/cdc2
-
Cdk1/cdc2 kinase
Upstate and Chemicon are Millipore
polyclonal
IgG
Rabbit
(liquid, tris-glycine saline, azide, glycerol)
-
Amphibian (various), Chicken, Sheep, Human, Mouse, Rat
immunogen = peptide (E G V P S T A I R E I S L L K E G G C) corresp. to residues 42-57 of human cdc2 kinase, coupled to KLH
cdc2 kinase
-
Cdk1/cdc2 kinase
Upstate and Chemicon are Millipore
kit
Unknown Host
(Kinase assay)
-
-
-
measures phospho- transferase activity of cdk1/cdc2 Kinase in immuno precipitatesand column fractions
-
Cdk2 (AN4.3)
Chemicon and Upstate are Millipore
monoclonal
IgG2a
Mouse
(liquid, PBS, azide, purified)
-
Human, Mouse, Xenopus
immunogen = recombinant cdk2
cdk2; no cross-reaction with other cdks.
-
Cdk2 (AN4.3)
Upstate and Chemicon are Millipore
monoclonal
IgG
Mouse
(liquid, tris-glycine saline, azide, glycerol, purified)
-
Human, Mouse, Xenopus
immunogen = recombinant human cdk2
cdk2, does not react with other cdks
-
Cdk2 (AOW10)
Upstate and Chemicon are Millipore
monoclonal
IgG
Rabbit
(liquid, tris-glycine saline, glycerol, azide, protein A purified)
-
Human
immunogen = synthetic peptide corresp. to the sequence C-...T K P V... (aa 287-298) of human cdk2, with an N-terminal cysteine added for conjugation purposes, KLH-conjugated
p33cdk2
-
Cdk2
Upstate and Chemicon are Millipore
polyclonal
IgG
Rabbit
(liquid, tris-glycine saline, azide, glycerol)
-
Human
immunogen = peptide corresp. to aa 287-298 (C-Q D V T K P V P H L R L) of human cdk2
human p33cdk2 protein kinase
-
Cdk4
Upstate and Chemicon are Millipore
polyclonal
IgG
Rabbit
(liquid, tris-glycine, NaCl, azide, protein G purified)
-
Human
immunogen = peptide corresp. in sequence to epitopes contained within the C-terminal domain of human cdk4
cdk4
-
Cdk4
Upstate and Chemicon are Millipore
polyclonal
IgG
Rabbit
(tris-glycine saline, glycerol, azide, affinity purified)
-
Human, Mouse
immunogen = synthetic peptide corresp. to aa 291-303
cdk4
-
Cdk5 (DC17)
Upstate and Chemicon are Millipore
monoclonal
IgG2a
Mouse
(protein A, Tris-glycine, azide)
-
Human, Mouse, Rat, Drosophila
immunogen = recombinant human cdk5
33 kDa Cdk5
-
Cyclin B1
Upstate and Chemicon are Millipore
monoclonal
IgG2b
Mouse
(liquid, Tris, NaCl, azide, protein-G purified)
-
Human, Mouse
immunogen = multiple clones, raised against recombinant human cyclin B1
human cyclin B1; does not cross-react with cyclin A or D
-