|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(liquid, serum, azide) |
IH |
Chicken, Lizard, Human, Monkey, Mouse, Rat |
immunogen = GABA- gluteraldehyde- BSA |
specifically stains GABA-ergic neurons. Stellate and basket cells of the cerebellum, as well as their processes are intensely stained; also GABA-ergic nerve terminals in the granular layer of cerebellum |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(PBS, azide, affinity purified) |
EM, IH |
Mammal (various) |
immunogen = GABA prepared with glutaraldehyde, conj. KLH |
GABA |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Guinea Pig
(serum) |
IH |
Mammal (various) |
immunogen = GABA prepared with glutaraldehyde, conj. KLH |
GABA |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
|
Rabbit
(PB, thimerosal, purified) |
IH |
Rat |
immunogen = GABA (no gluteraldehyde) |
GABA (gamma-aminobutyrate) |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(purified) |
ELISA, IH, WB |
Mammal (various) |
immunogen = GABA-coupled to BSA with glutaraldehyde |
GABA (gamma-aminobutyrate); no cross reaction with other amino acids |
- |
|
|
Sigma Aldrich |
monoclonal
IgG2a |
Mouse
(buffered aqueous solution, protein-A purified Ig, PBS, azide) |
IH, WB |
Mammal (various) |
immunogen = purified rat brain GAD 65 |
recognizes the lower molecular weight isoform of the two GAD isoforms identified in brain, GAD 65. Can be used for immunochemical localization of GABA secreting neurons |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
IgG |
Rabbit
(liquid, affinity purified Ig, PBS, azide) |
WB |
Mouse, Rat |
immunogen = recombinant protein from amino acids that are specific to the GAD67 protein |
Reacts with the 67kDa isoform of Glutamate Decarboxylase (GAD67) |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
IgG |
Rabbit
(liquid, affinity purified Ig, PBS, BSA, azide) |
WB |
Rat |
immunogen = synthetic peptide |
Reacts with the 67kDa isoform of Glutamate Decarboxylase (GAD67) |
- |
|
|
R&D Systems |
polyclonal
IgG |
Goat
(filtered solution, PBS, trehalose, lyophilized) |
ELISA, IH, WB |
Human |
immunogen = E. coli-derived rhGAD1(aa 2-97) |
recognizes GAD1 |
- |
|
|
R&D Systems |
polyclonal
IgG |
Goat
(filtered solution, PBS, BSA, biotin, lyophilized) |
WB |
Human |
immunogen = E. coli-derived rhGAD1(aa 2-97) |
recognizes GAD1 |
- |
|
|
R&D Systems |
monoclonal
IgG1 |
Mouse
(filtered solution, PBS, trehalose, lyophilized) |
IC, WB |
Human |
immunogen = E. coli-derived rhGAD1(aa 1-97) |
recognizes GAD1 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = peptide mapping at the C-terminus of GAD-67 of human origin |
recognizes GAD-67 and GAD-65 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, gelatin, azide) |
ELISA, IF, IP, WB |
Human, Mouse, Rat |
immunogen = amino acids 1-101 of GAD-67 of human origin |
recognizes GAD-67 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, PBS, gelatin, azide) |
ELISA, IF, WB |
Human, Mouse, Rat |
immunogen = peptide mapping at the N-terminus of GAD-67 of human origin |
recognizes GAD-67 |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum, thimerosal) |
ELISA, IC, IH |
- |
immunogen = GABA-glutaraldehyde-lysine conjugate |
GABA (gamma-aminobutyrate) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum, no preservative) |
IH, IM |
Rat |
immunogen = chemical / small molecule conj. to KLH via glutaraldehyde |
GABA (gamma-aminobutyrate) |
- |
|
|
Abcam |
polyclonal
IgG |
Guinea Pig
(liquid, whole antiserum, no preservative) |
IC, IF, IH |
- |
immunogen = chemical / small molecule conj. to KLH via glutaraldehyde |
GABA (gamma-aminobutyrate) |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum, azide) |
ELISA, IC, IF, IH |
Rat |
immunogen = chemical / small molecule conj. to BSA via glutaraldehyde |
GABA (gamma-aminobutyrate); no reaction with glutamate, aspartate, beta alanine, tyrosine, taurine, glycine and alanine based on a competitive inhibition ELISA |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, whole antiserum) |
ELISA, IH |
- |
immunogen = chemical / small molecule by a glutaraldehyde linker |
GABA (gamma-aminobutyrate) |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
DB, ELISA |
- |
immunogen = purified GABA conj. to BSA |
GABA (gamma-aminobutyrate); No cross-reaction with L-a-aminobutyric acid, L-glutamic acid, L-aspartic acid, glycine, d-aminovaleric acid, L-threonine, L-glutamine, taurine, putrescine, L-alanine and carnosine |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
DB |
- |
immunogen = GABA conj. to BSA |
GABA (gamma-aminobutyrate) |
- |
|
|
Abcam |
polyclonal
IgY |
Chicken
(liquid, PBS, goat serum, thimerosal) |
IC |
- |
immunogen = 4-aminobutyrate cross-linked to purifed fraction V BSA with glutaraldehyde |
GABA (gamma-aminobutyrate); no cross-reaction with L-alanine, D/L-aspartate, agmatine, D/L-arginine, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, glycine, L-histidine, L-isoleucine, L-lysine, L-leucine, L-methionine, L-asparagine, L-ornithine, L-proline, L-serine, taurine, L-threonine, L-valine, L-tryptophan, L-tyrosine |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
FC, IC, IH, IP, WB |
Human, Mouse, Rat |
immunogen = fusion protein of MBP with the aa seq. representing the cytosolic loop of the rat GABA A receptor β-3 subunit |
detects GABA A receptor β-3 subunit; no known reactivity or homology with other GABA subunits |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(unpurified neat rabbit serum) |
WB |
Human, Mouse, Rat |
immunogen = N-terminal peptide of GABA-A receptor alpha 6 subunit |
reacts with the 57 kDa alpha 6 subunit; no known reactivity with other GABA subunits |
- |
|
|
Synaptic Systems |
monoclonal
IgG3 |
Mouse
(IgG, affinity purified, PBS, azide, lyophilized; also available fluorescence labelled, 131011C3) |
IC, IH, IP, WB |
Human, Monkey, Mouse, Rat |
Immunogen = synthetic peptide AEPPVEGDIHYQR (aa residues 75 - 87 in rat) coupled to KLH |
specific for mammalian VGAT; Antigen identical in rat and Macaca fascicularis, 1 mismatch in mouse and human |
- |
|
|
Synaptic Systems |
polyclonal
|
Rabbit
(serum, RSA, lyophilized; also available affinity purified, 131 003) |
IC, IH, IP, WB |
Human, Monkey, Mouse, Rat |
Immunogen = synthetic peptide AEPPVEGDIHYQR (aa residues 75 - 87 in rat) coupled to KLH |
Specific for the mammalian vesicular GABA transporter; best and most specific marker for GABAergic synapses |
- |
|
|
Synaptic Systems |
polyclonal
|
Rabbit
(afffinity purified serum, RSA, lyophilized; also available fluorescence labelled) |
IB, IC, IH |
Human, Mouse, Rat |
Epitope = Synthetic peptide HSLEGLIEA YRTNAED (aa 510 - 525 in rat) coupled to KLH |
Epitope identical in rat and mouse; can be used for labeling of recycling synaptic vesicles at inhibitory nerve terminals by adding it to living neurons. |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(affinity purified antisera, PBS, azide) |
WB |
Human, Mouse, Rat |
immunogen = synthetic peptide corresp. to amino acids 1-20 located at the N-terminus of mouse VGAT |
detects VGAT |
- |
|
|
AnaSpec |
polyclonal
|
Rabbit
(liquid, affinity purified, PBS, BSA, azide) |
IH |
Human, Rat |
immunogen = a synthetic peptide derived from the C-terminus of human GAD65 protein |
detects GAD65 |
- |
|
|
AnaSpec |
polyclonal
|
Rabbit
(liquid, affinity purified, PBS, BSA, azide) |
IH |
Human, Mouse, Rat |
immunogen = a synthetic peptide near the N-terminus of mouse GAD67 protein |
detects GAD67 |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG2a |
Mouse
(phosphate buffer, protein-A purified Ig, azide) |
IH, WB |
Human, Mouse, Rat |
immunogen = recombinant fusion protein containing the unique N terminal regions of GAD67 not shared by GAD65 protein |
Reacts with the 67kDa isoform of Glutamate Decarboxylase (GAD67) |
- |
|
|
Synaptic Systems |
monoclonal
IgG1 |
Mouse
(purified IgG, lyophilized) |
ELISA, IC, IH, IP, WB |
Human, Mouse, Rat |
immunogen = purified recombinant doublet of E-Domain (aa 307 - 735) (epitope: C6 domain located in the first half of the E-domain) |
detects all splice variants that contain a complete E-domain including the C6 domain |
- |
|
|
Synaptic Systems |
polyclonal
|
Rabbit
(affinity purified with the immunogen, lyophilized) |
IP |
Human, Monkey, Mouse, Rat |
Immunogen = synthetic peptide AEPPVEGDIHYQR (aa residues 75 - 87 in rat) coupled to KLH |
Specific for vGAT |
- |