Ribonucleotide Reductase (AD203)
Chemicon and Upstate are Millipore
monoclonal
IgG1
Mouse
(liquid, purified Ig, Tris, saline, azide)
-
Human
Immunogen = human ribonucleotide reductase
ribonucleotide reductase (M1 subunit)
-
PARP
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(liquid, Tris, saline, azide, protein-A purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide (GVDEVAKKK-C) corresp. to the region downstream from the Caspase-3 cleavage site of human PARP1, with terminal cysteine added for conj. to KLH
antibody recognizes both the 116 kDa intact PARP1 as well as the 85 kDa fragment
-
PARP-1
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(liquid, serum, azide)
-
Bovine, Human, Mouse, Rat
based on the sequence from the C-terminal portion of the bovine PARP automodification domain (aa. 509-524)
116 kDa PARP and the 85 kDa apoptosis-related cleavage fragment
-
PARP-1 (p116/p25) (Y17)
Chemicon and Upstate are Millipore
monoclonal
Rabbit
(liquid, Tris, saline, BSA, glycerol, azide, purified)
-
Human
immunogen = synthetic peptide corresp. to residues before the cleavage site of human PARP-1
antibody only recognizes full-length form (p116) of PARP-1
-
PARP-1 (cleaved p25) (E51)
Chemicon and Upstate are Millipore
monoclonal
Rabbit
(liquid, Tris, saline, BSA, glycerol, azide, purified)
-
Human, Mouse
immunogen = synthetic peptide corresp. to residues before the cleavage site of human PARP-1
antibody only recognizes the p25 cleaved-form of PARP-1
-
PARP (5A5)
None
monoclonal
IgG1 ,κ
Mouse
(affinity purified, PBS, azide, Biotin conj.)
-
Human, Mouse
immunogen = recomb. (partial), N-terminal 2/3 sequence of PARP
recognizes the N-terminal region of human and mouse PARP
discontinued
PARP (5A5)
BioLegend
monoclonal
IgG1 ,κ
Mouse
(affinity purified, PBS, azide)
-
Human, Mouse
immunogen = recomb. (partial), N-terminal 2/3 sequence of PARP
recognizes the N-terminal region of human and mouse PARP
-
TdT (SPM149)
Novus Biologicals
monoclonal
IgG2a
Mouse
(liquid, ascites, BSA, PBS, azide)
-
Human
immunogen = full length human protein
-
-
TdT
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, azide)
-
Human, Rat
immunogen = synthetic cyclic peptide (common to human terminal deoxynuleotidyl transferase (TdT) protein
TdT
-
TdT (SPM149)
Novus Biologicals
monoclonal
IgG2a
Mouse
(prediluted)
-
Human
immunogen = full length human protein
-
-
TdT (SEN28)
Novus Biologicals
monoclonal
IgG1 κ
Mouse
(prediluted)
-
Human
immunogen = full length native human protein (purified)
-
-
PARP (5A5)
Novus Biologicals
monoclonal
IgG1 ,κ
Mouse
(liquid, protein-G purified ascites, PBS, azide)
-
Human, Mouse
immunogen = recomb. fragment, human
PARP; detects a band of approximately 113 kDa
-
PARP (C-2-10)
Novus Biologicals
monoclonal
IgG1
Mouse
(protein-A purified, PBS, BSA, azide)
-
Human, Monkey, Hamster, Mouse, Rat
immunogen = purified calf thymus PARP; epitope is in the C-terminal part of the DNA binding domain of PARP
recognizes both intact (116 kDa) PARP and the 85 kDa cleavage fragment.
-
PARP (C-2-10)
Novus Biologicals
monoclonal
IgG1
Mouse
(ascites, azide)
-
Human, Monkey, Hamster, Mouse, Rat
immunogen = purified calf thymus PARP; epitope is in the C-terminal part of the DNA binding domain
recognizes both intact (116 kDa) PARP and the 85 kDa cleavage fragment.
-
PARP
Novus Biologicals
polyclonal
Rabbit
(serum, azide)
-
Bovine, Human, Mouse, Rat
immunogen = synthetic peptide based on sequence from C-terminal part of the bovine PARP auto modification domain (aa. 509-524)
both intact (116 kDa) PARP and the 85 kDa cleavage fragment.
-
PARP
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, protein-A, PBS, azide)
-
Human, Mouse, Rat
immunogen = synthetic peptide from the N-terminal region of human PARP
reacts with 116 kDa PARP
-
PARP
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, affinity purified, BSA, PBS, azide)
-
Human, Mouse, Rat
immunogen = synthetic peptide, human, N-terminal
PARP
-
PARP, cleaved
Novus Biologicals
polyclonal
IgG
Rabbit
(BSA, PBS, glycerol, azide)
-
Cow, Human
immunogen = synthetic peptide corresp. to N-terminus of cleavage site (214/215) of human PARP
recognizes the 85 kDa fragment of cleaved PARP and can be used as marker for detecting apoptotic cells
-
PARP, cleaved
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide)
-
Cow, Human
immunogen = synthetic peptide corresponding to N-terminus of cleavage site (214/215) of human PARP
detects 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.
-
PARP1 (3G4)
Novus Biologicals
monoclonal
IgG2a ,κ
Mouse
(liquid, purified, no preservative)
-
Human
immunogen = partial recombinant PARP1, aa. 1~100, with GST tag
PARP1 - poly (ADP-ribose) polymerase family, member 1
-
PARP2
Novus Biologicals
polyclonal
IgG
Goat
(liquid, sera, tris-saline, BSA, azide, purified)
-
Dog, Human, Mouse, Rat
immunogen = synthetic peptide: C-L D L F E V E K D G E K E, representing the internal region of the human protein according to NP_005475.1
PARP2
-
PARP2
Novus Biologicals
polyclonal
Rabbit
(liquid, serum, azide)
-
Human
immunogen = N-terminus of human PARP 2
Reacts with residues 43-59 [Q R Q E S K K M P V A G G K A N K] of the 62 kDa human PARP-2 protein
-
PARP4
Novus Biologicals
polyclonal
IgG
Goat
(liquid, tris-saline, BSA, azide, purified)
-
Dog, Human, Mouse, Rat
immunogen = Synthetic peptide, D T H E L K Q K R T D C, representing the internal region of the human protein; NP_006428.1
PARP
-
PARP4
Novus Biologicals
polyclonal
Mouse
(liquid, sera, glycerol, no preservative)
-
Human
immunogen = PARP4 (NP_006428, 31-141 aa) partial recombinant protein with GST tag
PARP4 - poly (ADP-ribose) polymerase family, member 4
-
PARP1
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, affinity purified antisera, Tris-saline, BSA, azide)
-
Human
immunogen = a region between residue 1 and 50 of human PARP1
PARP1 - poly (ADP-ribose) polymerase family, member 1
-
PARP1
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, affinity purified antisera, Tris-saline, BSA, azide)
-
Human
immunogen = a region between residue 50 and 100 of human PARP1
PARP1 - poly (ADP-ribose) polymerase family, member 1
-
TIP60/KAT5 (T4D2)
Abcam
monoclonal
IgG
Mouse
(liquid, ascites, no preservative)
-
Human
immunogen = Ni-NTA purified truncated recombinant Tip60 (tagged) expressed in E. coli strain BL21 (DE3)
TIP60
-
TIP60/KAT5
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
-
Human
immunogen = synthetic peptide derived from within residues 450 to the C-terminus of human Tip60, conj. to KLH
TIP60
-
TIP60 (pS86)/KAT5
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse
immunogen = synthetic phosphopeptide derived from human KAT5 / Tip60 around the phosphorylation site of serine 86 (P G Sp R P)
endogenous levels of KAT5 / Tip60 only when phosphorylated at serine 86
-
TIP60/KAT5
Abcam
polyclonal
IgG
Rabbit
(liquid, tris-glycine saline, glycerol, azide, protein A purified)
-
Human, S. cerevisiae
immunogen = synthetic peptide: E N K V G S P E K P L S D L G, corresp. to aa331-345 of yeast ESA1 encompassing a conserved sequence in MYST family members MOZ, YBF2/SAS 3, SAS 2, Tip60, and MOF
recognizes yeast ESA1 and other mammalian MYST family members
-
TIP60/KAT5
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
-
Human, Mouse
immunogen = synthesized non phosphopeptide derived from human KAT5/Tip60 around the phosphorylation site of serine 86 (P G Sp R P)
detects endogenous levels of total KAT5/Tip60
-
TIP60/KAT5
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, ammonium sulfate ppt)
-
Human
immunogen = synthetic peptide selected from the N- terminal region of human KAT5 / Tip60, conj. to KLH
detects endogenous levels of total KAT5/Tip60
-
TIP60/KAT5
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, protein G purified)
-
Human
immunogen = synthetic peptide selected from the C-terminal region of human Tip60 conjugated to KLH
KAT5/Tip60
-
TIP60 (2455C3a)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, azide, BSA)
-
Human
immunogen = recombinant protein corresp. to an internal region of human TIP60
TIP60
-
TIP60 (3F9G7)
Santa Cruz
monoclonal
IgG2b
Mouse
(liquid, PBS, azide, BSA)
-
Human
immunogen = recombinant protein corresp. to aa18-208 of human TIP60
TIP60
-
TIP60 (3557C4a)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, azide, BSA)
-
Human
immunogen = recombinant protein corresp. to an internal region of human TIP60
TIP60
-
TIP60 (H-93)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, BSA)
-
Human, Mouse, Rat
immunogen = epitope corresp. to aa421-513 of human TIP60
TIP60
-
TIP60 (T4D2)
Santa Cruz
monoclonal
IgG2b
Mouse
(liquid, PBS, azide, BSA)
-
Human
immunogen = recombinant human TIP60
TIP60
-
TIP60 (3F9G7)
Novus Biologicals
monoclonal
IgG2b
Mouse
(liquid, ascites, azide, purified)
-
Human
immunogen = Ni-NTA purified truncated recombinant TIP60-gst expressed in E. coli strain BL21 (DE3)
TIP60
-
TIP60/KAT5
Upstate and Chemicon are Millipore
polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, protein A purified)
-
Human, Mouse
immunogen = synthetic peptide corresp. to aa494-513 (R I D S K C L H F T P K D W S K R G K W) of human Tip60 , conj. KLH
recognizes Tip60, Mr 60 kDa. Additional proteins were detected at 66 kDa and 80 kDa in some human and mouse cell lines
-
TIP60/KAT5
US Biological
polyclonal
IgG
Rabbit
(liquid, Tris-glycine, NaCl, azide, protein A purified)
-
Human, Mouse
immunogen = synthetic peptide corresp. to aa494-513 (R I D S K C L H F T P K D W S K R G K W) of human Tip60 , conj. KLH
recognizes Tip60, Mr 60 kDa. Additional proteins were detected at 66 kDa and 80 kDa in some human and mouse cell lines
-
TIP60/KAT5 (8A260)
US Biological
monoclonal
Mouse
(liquid, ascites, PBS, azide, glycerol)
-
Human, Mouse
immunogen = Ni-NTA purified truncated recombinant TIP60-fusion protein expressed in E. coli strain BL21 (DE3)
recognizes Tip60, Mr 60 kDa. Additional proteins were detected at 66 kDa and 80 kDa in some human and mouse cell lines
-
Ribonucleotide reductase
Novus Biologicals
polyclonal
IgY
Chicken
(affinity purified antisera, PBS, no preservative)
-
Human
immunogen = a sequence from 1a.a. to 92a.a. from RRM2, a 389a.a. long protein
detects Ribonucleotide reductase
-
TIP60 (N-17)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, BSA, affinity purified)
-
Human, Mouse, Rat
immunogen = epitope mapping near the N-terminus of human TIP60
TIP60
-
TIP60 (K-17)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, BSA, affinity purified)
-
Human, Mouse, Rat
immunogen = epitope mapping near the C-terminus of human TIP60
TIP60
-
PARP [H-250]
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = aa. 764-1014 mapping at the C-terminus of human PARP
reacts with PARP-1 and to a lesser extent with PARP-2
-
PARP
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, BSA, azide)
-
Human, Mouse, Rat
immunogen = a synthetic peptide derived from the N-terminus of human PARP protein
detects PARP
-
P53r2 NT
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human, Mouse, Rat
immunogen = a synthetic peptide corresponding to amino acids near the N-terminus of human p53r2
detects P53r2
-
TdT
Anaspec
monoclonal
IgG2a
Mouse
(supernatant, PBS, BSA, azide)
-
Human
immunogen = a recombinant protein derived from the N-terminus of human TDT
detects TdT
-
PARP (C-2-10)
Calbiochem EMD Biosciences
monoclonal
IgG1
Mouse
(purified, PBS, BSA, azide)
-
Human, Monkey, Hamster, Mouse, Rat
C-terminus of PARP DNA-binding domain of purified calf thymus PARP
full length (115 kDa) PARP and the 85-90 kDa apoptosis-related cleavage fragment
-
PARP (F1-23)
Calbiochem EMD Biosciences
monoclonal
IgG
Mouse
(liquid, undiluted ascites, azide)
-
Bovine, Human
immunogen within the zinc finger II DNA-binding domain of purified calf thymus PARP
zinc finger II domain with the full length ~116 kDa PARP, and the ~24 kDa cleavage fragment
-
PARP
Calbiochem EMD Biosciences
polyclonal
IgG
Rabbit
(liquid, undiluted serum, azide)
-
Human
immunogen = synthetic peptide (KQLPGVKSE GKRKGDEVD) corresponding to amino acids 197-214 of the human PARP protein
detects uncleaved 110 kDa human PARP protein or the cleaved 29 kDa fragment
-
PARP
Calbiochem EMD Biosciences
polyclonal
IgG
Rabbit
(liquid, undiluted serum, azide)
-
Human
immunogen = synthetic peptide (GVDEVAKKKSKKEK) corresp. to aa 215-228 of the human PARP protein
detects uncleaved 110 kDa human PARP protein or the cleaved 85 kDa fragment
-
PARP
Calbiochem EMD Biosciences
polyclonal
IgG
Rabbit
(liquid, undiluted serum, azide)
-
Bovine, Human, Monkey, Hamster, Mouse, Rat
immunogen = synthetic peptide (SKGPVKEEGTNKSEKR) corresponding to amino acids 509-524 of the bovine PARP protein automodification domain
detects PARP protein
-
PARP-1
Calbiochem EMD Biosciences
polyclonal
IgG
Rabbit
(liquid, undiluted serum, azide)
-
Bovine, Human, Monkey, Gerbil, Mouse, Rat
immunogen = full-length PARP-1 protein purified from bovine thymus.
detects PARP-1 protein
-
PARP-1
Calbiochem EMD Biosciences
polyclonal
IgG
Rabbit
(purified, PBS, gelatin, azide)
-
Human
immunogen = a synthetic peptide (SESSDKLY RVEYAKSG) corresp. to amino acids at the N-terminus of human PARP
detects full-length (115 kDa) PARP and the 24-31 kDa apoptosis-related cleavage fragment
-
PAR
Calbiochem EMD Biosciences
polyclonal
IgG
Rabbit
(liquid, undiluted serum, azide)
-
Human
immunogen = Poly (ADP-Ribose) with methylated BSA
detects polymers ranging in size from 2Ð300 pADPr units; some cross-reactivity with BSA (~67 kDa).
-
PARP-1
Orbigen
polyclonal
Rabbit
(serum, affinity purified, PBS, azide)
-
Human
reacts with N-terminus of human PARP-1
-
TdT
DAKO
polyclonal
Rabbit
(serum, affinity purified, stabilizing protein,Tris-HCL, azide)
-
Cat, Chicken, Calf, Human, Mouse, Rat
immunogen = purified TdT isolated from calf thymus
recognizes TdT, nuclear staining; major peptide of 58-60 kDa on Western blots
-
TdT (HT-6, 4KB128, UCHT1)
DAKO
monoclonal
IgG1 κ
Mouse
(FITC, RPE and APC. Purified)
-
Human
-
Triple-Colour Reagent, Anti-Human TdT/FITC Anti-Human CD22/RPE Anti-Human CD3/APC; simultaneous detection and enumeration of T and B-lymphocyte precursor cells, B cells and T cells
-
TdT
DAKO
polyclonal
Rabbit
(serum, affinity purified, stabilizing protein,Tris-HCL, azide, ready to use together with Autostainer Link instruments)
-
Human
immunogen = purified TdT isolated from calf thymus
has been found to react with lower molecular weight forms of the enzyme
-
TdT
DAKO
polyclonal
Rabbit
(liquid, stabilizing protein,Tris-HCL, azide, ready to use together with Autostainer /Autostainer Plus instruments)
-
Human
immunogen = purified TdT isolated from calf thymus
has been found to react with lower molecular weight forms of the enzyme
-
TdT (SEN28)
Diagnostic Biosystems
monoclonal
IgG2a
Mouse
(pretitered, ready to use)
-
Human
immunogen = recomb. protein corresponding to the amino terminal region of the TdT molecule
stains TdT, 60kD peptide in human lymphoblasts; location nuclear
-
TdT (SEN28)
Diagnostic Biosystems
monoclonal
IgG2a
Mouse
(tissue culture supernatant, azide)
-
Human
immunogen = recomb. protein corresponding to the amino terminal region of the TdT molecule
stains TdT, 60kD peptide in human lymphoblasts; location nuclear
-