|
|
anti PINK1J.W. Pridgeon(Paper),Reference: Pridgeon, 2007 |
polyclonal
|
Rabbit
|
WB |
Human |
immunogen = amino acid residues 135–155 of human PINK1 |
specifically recognizes endogenous PINK1 as well as recombinant wild-type and mutant PINK1 proteins |
- |
|
|
anti PINK1D.S. Park, Reference: Haque, 2008 |
polyclonal
|
Rabbit
|
WB |
Human, Mouse |
immunogen = amino acid residues 125–149 of Pink1 |
recognizes endogenous and wild-type PINK1 |
- |
|
|
Michael J.Fox Foundattion, working with Epitomics,Inc.(Available free to researchers!)Cat# MJFF-1MJFF-2MJFF-3 |
monoclonal
|
Rabbit
|
WB |
Human, Mouse |
immunogen = the LRRK2 fragment available from Invitrogen (Amino acids 970-2527)Investigators from Eli Lilly and Company (Ryder JW and Merchant KM, Translational Science) and Johns Hopkins University (Li X, Dawson VL and Dawson TM), MJFF conducted preliminary studies to select the best performing hybridomas |
Recognizes LRRK2 |
- |
|
|
Michael J.Fox Foundattion, working with Epitomics,Inc.(Available free to researchers!)Cat# MJFF-4MJFF-5 |
monoclonal
|
Rabbit
|
WB |
Human, Mouse |
immunogen = the LRRK2 fragment available from Invitrogen (Amino acids 970-2527)Investigators from Eli Lilly and Company (Ryder JW and Merchant KM, Translational Science) and Johns Hopkins University (Li X, Dawson VL and Dawson TM), MJFF conducted preliminary studies to select the best performing hybridomas |
Recognizes LRRK2 |
- |
|
|
Molecular Genetics Laboratory and Core, Morris K. Udall Parkinson’sDisease Research Center of Excellence, Mayo Clinic, Jacksonville, FLantibody # Ab 04/11Reference: Melrose, 2007 |
polyclonal
|
Rabbit
(affinity purified) |
IH, WB |
Human, Mouse |
immunogen = synthetic peptide from human LRRK2 (within C-terminal amino acid residues 2507–2527) |
Recognizes 286 kDa LRRK2 |
- |
|
|
Molecular Genetics Laboratory and Core, Morris K. Udall Parkinson’sDisease Research Center of Excellence, Mayo Clinic, Jacksonville, FLantibody # PA0362Reference: Melrose, 2007 |
polyclonal
|
Rabbit
(affinity purified) |
IH, WB |
Human, Mouse |
immunogen = synthetic peptide from human LRRK2 (within C-terminal amino acid residues 2507–2527) |
Recognizes 286 kDa LRRK2 |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
IgG |
Sheep
(serum, lyophilized) |
IH |
Human |
immunogen = synthetic peptide from human LRRK2 protein |
Recognizes LRRK2 |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
IgG |
Rabbit
(serum, liquid) |
ELISA, WB |
Human, Mouse |
immunogen = synthetic peptide from rat LRRK2 |
Recognizes ~286 kDa LRRK2 |
- |
|
|
Zymed/ Invitrogen |
polyclonal
|
Rabbit
(affinity purified antiserum, PBS, azide) |
WB |
Human |
immunogen = synthetic peptide derived from the N-terminal region of the human PINK1 protein |
~63 kDa PINK1 |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(antisera, azide) |
WB |
- |
immunogen = a synthetic peptide made to the C-terminal region of the mouse Pink1 protein sequence (between residues 550-580) |
expect PINK1; immunogen has 100% homology with mouse and rat protein, 93% homology with human protein |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(antisera, BSA, TBS, glycerol, azide) |
IH, WB |
Human, Mouse, Rat |
immunogen = synthetic peptide (LVRALLQREA SKRPSARVAA N) corresp. to aa 484-504 of human PINK1 |
PINK1; detects a band of approximately 66 kDa |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(antisera, TBS, BSA, azide) |
WB |
- |
immunogen = synthetic peptide made to the human Pink1 protein sequence (between residues 175-250) |
theoretical molecular weight of human PNK1 is ~60 kDa |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(affinity purified antisera, Tris, azide) |
IH, WB |
Human |
immunogen = internal synthetic peptide made to the human LRRK2 protein sequence (betweenresidues 900-1000) |
specific for ~286 kDa LRRK2; 99% homology of immunogern with mouse |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(affinity purified antisera) |
IF, IH, WB |
Bovine, Human |
immunogen = C-terminal synthetic peptide made to the human LRRK2 protein sequence (betweenresidues 2500-2527) |
specific for ~286 kDa LRRK2; 87% homology of immunogern with rat |
- |
|
|
Novus Biologicals |
polyclonal
IgG |
Rabbit
(purified antisera) |
WB |
Human |
immunogen = synthetic peptide made residues 258-274 (YRKSKRGPK QLAPHPNI) of human PINK1 |
reacts with residues 258-274 of PINK1; tested on transfected lysates, endogenous protein detection is unknown |
- |
|
|
Novus Biologicals |
polyclonal
|
Rabbit
(affinity purified antisera, Tris, azide) |
WB |
Human |
immunogen = N-terminal region synthetic peptide made to the human Pink1 protein sequence (between residues 1-50) |
detectsPINK1 ~60 kDa by WB |
- |
|
|
Abcam |
monoclonal
IgG1, κ |
Mouse
(liquid, Protein-G purified, PBS, no preservative) |
WB |
Human |
immunogen = recombinant fragment corresponding to amino acids 2161-2260 of Human LRRK2 |
tested in WB against the recombinant fragment used as immunogen; we have no data on the detection of endogenous protein |
- |
