|
|
None |
polyclonal
|
Rabbit
(PBS, no preservatives, stabilizers or carriers, affinity purified) |
ELISA, IB |
- |
- |
specific for the soluble fragment resulting from the α-secretase cleavage of APP. It has no cross reactivity to sAPPβ β-amyloid or full length APP |
Discontinued |
|
|
BioLegend |
polyclonal
IgG |
Rabbit
(PBS, no preservatives, stabilizers or carriers, affinity purified) |
ELISA |
- |
- |
specific for the soluble fragment cleaved n-terminus to the β-secretase cleavage site of APP. It has negligible cross reactivity to sAPPα or full length APP |
Available from BioLegend |
|
|
BioLegend |
monoclonal
IgG1 |
Mouse
(purified, ascites, PBS, no preservatives or carrier proteins) |
ELISA, IH, WB |
Human |
Epitope lies within amino acids 61-77 of amyloid precursor protein |
APP |
Available from BioLegend |
|
|
BioLegend |
monoclonal
IgG1 |
Mouse
(purified, PBS, no preservatives or carrier proteins, biotinylated) |
ELISA, IH, WB |
Human |
Epitope lies within amino acids 573-596 of amyloid precursor protein |
APP |
Available from BioLegend |
|
|
BioLegend |
monoclonal
IgG1 |
Mouse
(liquid, protein G purified, no preservative) |
ELISA, IH, WB |
- |
- |
recognizes all three known forms of APP (amyloid precursor protein), β-APP695, β-APP751 and β-APP770 |
Available from BioLegend |
|
|
BioLegend |
monoclonal
IgG2b |
Mouse
(crude ascites) |
ELISA, IH, IP, WB |
Human, Mouse |
epitope within amino acids 18-23 of the Aβ peptide |
reactive to amino acids 17-24 of Aβ and to APP |
Available from BioLegend |
|
|
BioLegend |
monoclonal
IgG2b |
Mouse
(purified PBS thimerosal no BSA) |
ELISA, IH, IP, WB |
Human, Mouse |
amino acid residue 17-24 of beta Amyloid peptide |
reactive to aa 17-24 Aβ and to APP |
Available from BioLegend |
|
|
BioLegend |
monoclonal
IgG2a |
Rat
(liquid, raw ascites) |
IF, IP, WB |
Dog, Human, Chicken |
immunogen = bovine and mouse muscle neuronal nicotinic acetylcholine receptors |
α1 subunits of muscle AChRs; also binds to α3 and α5 subunits of (native only) human neuronal AChRs, so not for blotting |
Available from BioLegend |
|
|
BioLegend |
monoclonal
IgG2a |
Rat
(liquid, raw ascites) |
IF, IP |
Human, Mouse, Electrophorus, Torpedo |
immunogen = denatured Electro- phorus electricus acetylcholine receptors |
cytoplasmic surface of native and denatured muscle AChR α1 subunits within amino acids 371-386. Useful in studying AChR synthesis and assembly |
Available from BioLegend |
|
|
None |
monoclonal
IgG2aκ |
Rat
(liquid, raw ascites) |
IF, IP, WB |
Chicken |
immunogen = bacterially expressed large cytoplasmic domain of chicken neuronalnicotinic acetylcholine receptors (AChR) α3 subunits |
cytoplasmic surface of chicken neuronal (AChR) α3 subunits within amino acids 315-411, both native and denatured |
Discontinued |
|
|
None |
monoclonal
IgG1κ |
Rat
(liquid, raw ascites) |
IF, IP |
Dog, Human, Rodent (various), Chicken |
immunogen = denatured Electro- phorus electricus acetylcholine receptors |
α1 subunits of muscle AChRs; also binds to α3 and α5 subunits of human neuronal AChRs |
Discontinued |
|
|
BioLegend |
monoclonal
IgG1κ |
Rat
(liquid, raw ascites) |
IF, IP, WB |
Human, Rodent (various), Chicken |
immunogen = purified rat brain neuronal nicotinic acetylcholine receptors (AChR) |
extracellular surface of α4 AChR subunits; binds best to native but will work on immunoblots of denatured α4. |
Available from BioLegend |
|
|
None |
monoclonal
IgG1 |
Rat
(liquid, raw ascites) |
WB |
Human, Rat, Chicken |
immunogen = purified chicken brain neuronal nicotinic acetylcholine receptors (AChRs) |
binds to denatured but not native α5 subunits of neuronal AChRs, predicted MW = 53kD |
Discontinued |
|
|
BioLegend |
monoclonal
IgG1κ |
Rat
(liquid, raw ascites) |
IF, IP, WB |
Human, Rat, Chicken |
immunogen = bacterially expressed large cytoplasmic domain of chicken α7 neuronal nicotinic acetylcholine receptors |
cytoplasmic surface of chicken α7 acetylcholine receptors between amino acid 365-384. Binds to both native and denatured α7 subunits, but does not bind to α8 |
Available from BioLegend |
|
|
None |
monoclonal
IgG2cκ |
Rat
(liquid, raw ascites) |
IP |
Chicken |
immunogen = purified chicken brain α8 neuronal nicotinic acetylcholine receptors |
extracellular surface of α8 subunits of chicken neuronal nicotinic AChR. Clone 305 has a high affinity for native α8 but not the denatured protein |
Discontinued |
|
|
None |
monoclonal
IgG2b |
Rat
(liquid, raw ascites) |
IF, IP, WB |
Chicken |
immunogen = bacterially expressed large cytoplasmic domain fragment of chicken α8 neuronal nicotinic acetylcholine receptor subunits |
cytoplasmic surface of both native and denatured α8 subunits of AChRs between amino acids 323-342 |
Discontinued |
|
|
None |
monoclonal
IgG1κ |
Rat
(liquid, raw ascites) |
IP |
Bovine, Human, Rodent (various) |
immunogen = purified bovine muscle nicotinic acetylcholine receptors |
extracellular surface of muscle AChR β1-subunits; useful in studying specificities of autoantibodies in myasthenia gravis (MG) patients |
Discontinued |
|
|
BioLegend |
monoclonal
IgG2aκ |
Rat
(liquid, raw ascites) |
IF, IP, WB |
Human, Rodent (various), Chicken |
immunogen = purified neuronal nicotinic acetylcholine receptors from chicken brain |
extracellular surface of neuronal AChR β2 and native subunits; binds to denatured subunits but with lower affinity. |
Available from BioLegend |
|
|
BioLegend |
monoclonal
IgG2a |
Rat
(liquid, raw ascites) |
IF, IP, WB |
Bovine, Human |
immunogen = purified bovine muscle nicotinic acetylcholine receptors |
binds to the γ-subunit of bovine AChR |
Available from BioLegend |
|
|
None |
monoclonal
IgG1 |
Mouse
(liquid, supernatant, PBS, BSA, azide) |
IH |
Human |
immunogen = muscle actin purified from human myocardium |
reacts with a 42 kDa protein specific for actin present in skeletal, cardiac and smooth muscle; stains tumors of smooth muscle (leiomyosarcomas, leimyomas) and skeletal muscle (rhabdomyosarcomas) |
Discontinued |
|
|
None |
monoclonal
IgG2a |
Mouse
(liquid, ascites, PBS, BSA, azide) |
IH |
Human |
immunogen = α-smooth muscle actin N-terminal decapeptide |
specific for the α-smooth muscle actin isoform |
Discontinued |
|
|
None |
monoclonal
IgM |
Mouse
(liquid, ascites) |
IB, IF, WB |
Human, Rat, Chicken |
immunogen = preparation of human blood platelet proteins eluted by ATP from an F-actin affinity column |
43kDa actin-associated protein (kaptin); binds to polymerized actin in vitro and stains the leading edge of platelets, fibroblast lamellipodia, epithelial stereocilia and neurite growth cones. |
Discontinued |
|
|
None |
monoclonal
IgG1 |
Mouse
(liquid, affinity purified) |
IH |
Human |
- |
actin isotypes α and γ (42kD) of skeletal, cardiac and smooth muscle; nonreactive with other mesenchymal and all epithelial cells, with the exception of myo-epithelium; labels rhabdomyosarcomas, hemangiopericytomas, leiomyosarcomas and leiomyomas; no reaction with non-myogenic sarcomas |
Discontinued |
|
|
None |
monoclonal
IgG2b |
Mouse
(liquid, affinity purified, PBS, azide) |
IH |
Human |
- |
actin isotypes α and γ (42kD) of smooth muscle, but not skeletal or cardiac muscle cells; nonreactive with other mesenchymal and all epithelial cells, with the exception of myo-epithelium; labels leiomyosarcomas and leiomyomas; not carcinoma or lymphoma |
Discontinued |
|
|
BioLegend |
monoclonal
IgG2a |
Mouse
(liquid, supernatant, Tris/HCl, azide) |
IC, IH, WB |
Cow, Human, Rat, Chicken |
immunogen = N-terminal synthetic decapeptide of α-smooth muscle actin coupled to KLH |
smooth muxcle actin, N-terminal region |
Available from BioLegend |
|
|
BioLegend |
monoclonal
IgM |
Mouse
(liquid, supernatant, Tris/HCl, azide) |
IB, IC, IH, WB |
Human, Rabbit, Mouse, Rat, Chicken |
immunogen = purified striated muscle actin from rabbit. |
α-skeletal (α-sarcomeric) and α-cardiac muscle actins. No labelling with actin from fibroblasts (α- smooth muscle and β-cytoplasmic actin), aorta (α-smooth muscle actin), or chicken gizzard (γ-smooth muscle actin) |
Available from BioLegend |
|
|
None |
polyclonal
|
Rabbit
(serum) |
WB |
Human |
- |
recognizes Amyloid beta 1-40; some (minimal) cross-reactivity to Amyloid beta 1-42 |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(affinity purified, PBS, BSA, thimerosol) |
ELISA, IH, WB |
Dog, Human, Chicken |
- |
recognizes Amyloid beta 1-42 and 1-40 |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(serum) |
WB |
Human |
- |
recognizes Amyloid beta 1-42; some (minimal) cross-reactivity to Amyloid beta 1-40 |
Discontinued |
|
|
None |
monoclonal
IgG2a |
Mouse
(liquid, ascites) |
ELISA, IH, IP, WB |
Human |
immunogen = beta-amyloid (epitope at amino terminus,aa 1-11) |
recognizes the Amyloid beta (Aß) 1-11 of the human beta amyloid peptide; also recognizes full length APP, sAPP species excluding sAPPß, C99 and Aß |
Discontinued |
|
|
BioLegend |
monoclonal
IgG1 |
Mouse
(crude ascites) |
ELISA, IH, IP, WB |
Human |
the epitope lies within amino acids 3-8 of beta amyloid (EFRHDS) |
reactive to aa 1-16 of Aβ and to APP; reacts to the abnormally processed isoforms, as well as precursor forms |
Available from BioLegend |
|
|
BioLegend |
kit
|
Unknown Host
(Colorimetric sandwich: Capture Antibody Coated Plate; HRP Detection Antibody; TMB substrate) |
ELISA |
Human, Rodent (various) |
- |
specific for the x-42 isoform of beta amyloid; negligible cross reactivity with the x-40 isoform of beta amyloid |
Available from BioLegend |
|
|
BioLegend |
kit
|
Unknown Host
(Colorimetric sandwich ELISA Kit: Capture Antibody Coated Plate; HRP Detection Antibody; TMB substrate) |
ELISA |
Human, Rodent (various) |
- |
reactive to Amyloid β x-40; negligible cross reactivity with the x-42 isoform of beta amyloid |
Available from BioLegend |
|
|
BioLegend |
monoclonal
IgG1 |
Mouse
(biotinylated IgG, PBS, no preservatives or carrier proteins) |
ELISA, IH, IP, WB |
Human |
the epitope lies within amino acids 3-8 of beta amyloid (EFRHDS) |
reactive to aa 1-16 Amyloid β; reacts to the abnormally processed isoforms, as well as precursor forms |
Available from BioLegend |
|
|
None |
monoclonal
IgG1 |
Mouse
(supernatant; PBS, azide, BSA, concentrate or prediluted) |
IH |
Human |
Immunogen = intermediate filament of bovine spinal cord |
GFAP 52kD; stains normal glial cells as well as astrocytomas and ependymomas. It does not react with vimentin, neurofilament, cytokeratin, or desmin |
Discontinued |
|
|
None |
monoclonal
IgG1 |
Mouse
(affinity purified) |
IH |
Human |
- |
GFAP Labels astrocytomas, glioblastomas and gliomas but not other neoplasms |
Discontinued |
|
|
BioLegend Cat# SMI-21R |
monoclonal
IgG1 |
Mouse
(ascites) |
ELISA, IH, WB |
Dog, Human, Monkey |
- |
Reacts with GFAP in astrocytes and Bergmann glia in human, monkey and dog paraffin sections. No reactivity in rat, rabbit or mouse brain sections. Other species have not been examined. No reactivity was observed in liver or kidney paraffin sections. SMI-21 reacts with GFAP and does not cross-react with other intermediate filaments by immunohistochemistry or western blot. |
Available from BioLegend |
|
|
BioLegend |
monoclonal
IgG2b |
Mouse
(ascites, azide) |
ELISA, IC, IH, WB |
Human |
Derived from the Bigner-Engclones Mab1B4 |
GFAP in astrocytes and Bergman glia also detects a more selected group of astrocytomas than SMI 23, 24 and 25 |
Available from BioLegend |
|
|
BioLegend |
monoclonal
IgG2b |
Mouse
(ascites, azide) |
IC, IH, WB |
Human |
Derived from the Bigner-Engclones Mab2E1 |
GFAP in astrocytes and Bergman glia, it detects a more selected group of astrocytomas than mixtures of SMA 23, 24 and 25. |
Available from BioLegend |
|
|
BioLegend |
monoclonal
IgG2b |
Mouse
(ascites, azide) |
ELISA, IC, IH, WB |
Human |
Derived from the Bigner-Engclones Mab4A11 |
GFAP in astrocytes and Bergman glia, it detects a more selected group of astrocytomas than miztures of SMI 23, 24 and 25 |
Available from BioLegend |
|
|
BioLegend |
monoclonal
IgG2b, IgG1 |
Mouse
(ascites) |
ELISA, IB, IC, IH |
Cow, Dog, Sheep, Pig, Human, Guinea Pig, Mouse, Rat, Chicken |
- |
- |
Available from BioLegend |
|
|
BioLegend |
monoclonal
IgG2b |
Mouse
(ascites fluid) |
ELISA, IC, IH, WB |
Cow, Dog, Sheep, Pig, Human, Guinea Pig, Mouse, Rat, Chicken |
- |
IC stains astrocytes and astrocytic processes as well as Bergman glia. Both anaplastic and reactive astrocytes are stained. Not stained are metastatic tumors and brain tumors of non-astrocytic origin, such as medulloblastomas, meningiomas, choroid plexus papillomas and schwannomas. |
Available from BioLegend |
|
|
None |
polyclonal
|
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
ELISA, IH, IP, WB |
Mammal (various) |
Immunogen = synthetic phospho-peptide corresp. to aa residues surrounding the phospho Thr 202 and Tyr 204 of MAP kinase |
ERK phosporylated Thr202/Tyr204 |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(liquid, PBS, BSA, glycerol, azide, affinity purified) |
WB |
Human, Mouse, Rat |
Immunogen = synthetic phospho-peptide derived from a region of human MEK1 that contains threonine 292. |
MEK1 phosphorylated threonine 292 |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
WB |
Human, Mouse, Rat |
Immunogen = synthetic phospho-peptide derived from a region of human MEK1 that contains threonine 386. |
MEK phosphorylated at Thr386 |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified) |
ELISA, IH, IP, WB |
Human |
Immunogen = synthetic phospho-peptide corresp. to aa residues surrounding the phosphor Serine 218/222 of human MEK 1/2. |
MEK1/2 phosphorylated at Ser218/222 |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
WB |
Mouse, Rat |
Immunogen = synthetic phospho-peptide derived from a region of mouse MEK2 that contains threonine 394 |
MEK2 phosphorylated at Thr394 |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(liquid, PBS, BSA, azide, affinity purified) |
WB |
Human |
Immunogen = synthetic phospho-peptide derived from a region of human MEK2 that contains threonine 394 |
MEK2 phosphorylated at Thr394 |
Discontinued |
|
|
None |
monoclonal
IgG1 |
Mouse
(purified) |
ELISA, WB |
Human, Various species, Yeast |
immunogen = denatured bovine ubiquitin |
recognizes ubiquitin, polyubiquitin, and ubiquitin-conjugated proteins |
Discontinued |
|
|
None |
monoclonal
IgG1 |
Mouse
(ascites) |
WB |
Human, Various species, Yeast |
immunogen = denatured bovine ubiquitin |
recognizes ubiquitin, polyubiquitin, and ubiquitin conjugated proteins |
Discontinued |
|
|
None |
monoclonal
IgG1,κ |
Mouse
(ascites, azide) |
ELISA, IH, WB |
Bovine, Human, Chicken, Drosophila, Nematode |
immunogen = purified KLH-conjugated ubiquitin |
ubiquitin; insensitive to formalin fixation, neurofibrillary tangles, Lewy bodies of PD and the Pick bodies in Pick's disease |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(liquid, antiserum) |
WB |
Non-specific |
immunogen = purified full-length cre recombinase |
reacts with Cre Recombinase; highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It can be used to determine the success of gene manipulations in transgenic animals |
Discontinued |
|
|
BioLegend |
polyclonal
|
Rabbit
(liquid, purified, PBS, thimerosal.Also available as antiserum, PRB-101C) |
IP, WB |
Non-specific |
immunogen = the twelve amino acid sequence CYPYDVPDYASL |
recognizes the same hemagglutinin flu epitope YPYDVPDYA as the monoclonal antibody, HA.11 clone 16B12 |
Available from BioLegend |
|
|
BioLegend |
monoclonal
IgG1 |
Mouse
(liquid, purified, PBS, glycerol, thimerosal.Also available as ascites, MMS-101R, Biotin, BIOT-101L,FITC, FITC-101L,AFC, AFC-101P,Alexa Fluor® 488, A488-101L Alexa Fluor® 594, A594-101L) |
IF, IH, IP, WB |
- |
immunogen = the twelve amino acid peptide CYPYDVPDYASL |
recognizes the influenza hemagglutinin epitope (YPYDVPDYA) located in the middle of protein sequences as well as at the N- or C-terminus |
Available from BioLegend |
|
|
None |
polyclonal
|
Rabbit
(crude antiserum) |
IF, WB |
Human, Monkey, Hamster, Mouse, Xenopus |
immunogen = C-terminus of the human γ-tubulin molecule (H A A T R P D Y I S W G T Q) |
γ-tubulin |
Discontinued |
|
|
None |
monoclonal
IgG2b; |
Mouse
(ascites) |
IH, WB |
Bovine, Mouse, Rat |
immunogen = acetylated α-tubulin from the axoneme of sea urchin sperm flagella |
acetylated α-tubulin from primary cilia, centrioles, mitotic spindles, midbodies and to subsets of cytoplasmic microtubules in 3T3 and HeLa cells |
Discontinued |
|
|
BioLegend |
monoclonal
IgG2a |
Mouse
(liquid, PBS, thimerosal, affinity purified, conj. AlexaFluor®488) |
IH, IP, WB |
Mammal (various) |
immunogen = microtubules derived from rat brain |
reactive to neuron specific class III β-tubulin (βIII), but does not identify β-tubulin found in glial cells |
Available from BioLegend |
|
|
None |
monoclonal
IgG |
Rabbit
(liquid, PBS, thimerosal, purified) |
IF, IP, WB |
Mammal (various) |
immunogen = microtubules derived from rat brain |
reactive to neuron specific class III β-tubulin (βIII), but does not identify β-tubulin found in glial cells |
Discontinued |
|
|
BioLegend |
monoclonal
IgM |
Mouse
(liquid, ascites, azide) |
ELISA, IH, WB |
Mammal (various) |
- |
β-tubulin, depolymerized |
Available from BioLegend |
|
|
BioLegend |
monoclonal
IgG1 |
Mouse
(liquid, ascites, azide) |
ELISA, IH, WB |
Mammal (various) |
- |
β-tubulin, polymerized |
Available from BioLegend |
|
|
None |
monoclonal
IgG1 |
Mouse
(ascites) |
IH, WB |
Bovine, Mouse, Rat |
immunogen = Sarkosyl- resistant filaments from sea urchin sperm axonemes |
all α-tubulin isoforms |
Discontinued |
|
|
None |
monoclonal
IgG1 |
Mouse
(liquid, supernatant) |
IF, WB |
Human |
immunogen = GST-fusion containing the complete N-terminal fragment of human PS2 (residues 1-87) |
presenilin 2 |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(liquid, affinity purified) |
IH, WB |
Human |
immunogen = human Aph-1aL/S Loop region 92-115 with seq C K L L K K A D E G L A S L S E D G R S P I S I R |
Aph-1aL/S loop |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(liquid, affinity purified) |
IH, WB |
Human |
immunogen = human Aph-1b/C-terminal region sequence C Q D K N F L L Y N Q R S R Q D K N F L L Y N Q R S R of human Aph-1b. |
Aph-1b; some reactivity with Aph-1aL/S |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(liquid, affinity purified) |
IF, IP, WB |
Human, Mouse |
immunogen = C-terminal fragment of nicastrin corresp. to a peptide containing residues 691-709 [C]-N A K A D V L F I A P R E P G A V S Y, conj. to KLH |
nicastrin |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(liquid, affinity purified) |
IF, IP, WB |
Human, Mouse |
immunogen = N-terminal fragment of nicastrin corresp. to a peptide containing residues 103-124 (C F T R D L M E K L K G R T S R I A G L A V S L), coupled to KLH |
nicastrin |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(liquid, serum) |
IP, WB |
Human |
immunogen = N-terminal residues of Pen-2 and recognizes the residues E R V S N E E K L N L of Pen-2 |
Pen2 (12kDa) |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(liquid, serum) |
IP, WB |
Human |
immunogen = C-terminal residues of Pen-2 and recognizes the residues F Q I Y R P R W G A L G D Y L S F T I P L G T P of Pen-2 |
Pen2 |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(affinity purified) |
IF, IP, WB |
Human |
immunogen = cytoplasmic loop (or C-terminal fragment of PS1) corresp. to a peptide containing residues 368-390[C I L A G E D P E E R G V K L G L G D F I F Y S] |
Presenilin 1 |
Discontinued |
|
|
None |
polyclonal
|
Rabbit
(affinity purified) |
IF, IP, WB |
Human |
immunogen = peptide 1-25 of the human PS1 C M T E L P A P L S Y F Q N A Q M S E D N H L S, coupled to KLH |
presenilin 1 |
Discontinued |