|
|
Calbiochem EMD Biosciences |
polyclonal
IgG |
Goat
(liquid, neat serum, no preservative) |
ELISA, IH |
Human |
immunogen = synthetic peptide (Y E V H H Q K L V F F A E D V) corresp. to aa 44-63 of human APP |
APP (~110-135 kDa) |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, supernatant, azide) |
IC, IH, IP, WB |
Cow, Pig, Human, Monkey |
immunogen = synthetic peptide corresp. to aa 1-17 of the mature human Amyloid Precursor Protein, conjugated to KLH. |
reacts with β-amyloid peptide and secreted APP (alpha-secretase cleaved); has very weak recognition of full length APP and we do not recommend it for this purpose; does however strongly recognize alpha-secretase cleaved soluble APP as well as b-amyloid peptide (40 and 42) |
- |
|
|
Zymed/ Invitrogen |
polyclonal
|
Rabbit
(liquid, affinity purified, PBS, azide) |
IH, IP, WB |
Human, Mouse |
immunogen = synthetic peptide derived from the C-terminal region of the human and mouse amyloid-β precursor protein (APP695) |
APP full length (~100kDa) and C99 C-terminal cleavage frag (~10-12kDa); cross-react with APLP2 |
- |
|
|
Zymed/ Invitrogen |
polyclonal
|
Rabbit
(affinity purified, PBS, azide) |
ELISA, IF, IH, WB |
Pig, Human, Mouse, Rat |
Immunogen = synthetic peptide of 22 aa from the C-terminus of human β-APP |
All three forms of full length APP and N-terminal truncated forms of β-APP. Detects C-terminal membrane fragment of β-APP that remains after α -or β-secretase cleavage |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, purified) |
IF, IH, WB |
Fish (various), Dog, Pig, Human, Monkey, Mouse, Rat |
immunogen = purified rhN-terminal epitope (aa 66-81) on the pre-A4 molecule, fusion protein |
human pre-A4 APP; recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa; cross reacts with APLP2 |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol) |
WB |
Human, Mouse, Rat |
Immunogen = synthetic phospho-peptide corresp. to residues around Thr389 of human p70 S6 kinase |
detects endogenous levels of p70 S6 kinase only when phosphorylated at threonine 389; also detects p85 S6 kinase when phosphorylated at the analogous site (Thr412), and possibly S6KII phosphorylated at Thr401 |
- |
|
|
Cell Signaling |
polyclonal
|
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide) |
WB |
Human, Monkey, Mouse, Rat |
Immunogen = synthetic phospho-peptide corresp. to residues around Thr389 of human p70 S6 kinase |
detects endogenous levels of p70 S6 kinase only when phosphorylated at threonine 389; also detects p85 S6 kinase when phosphorylated at the analogous site (Thr412), and possibly S6KII phosphorylated at Thr401 |
- |
|
|
IC16(Claus U. Pietrzik,Institute of Physiological Chemistry and Pathobiochemistry, MolecularNeurodegeneration, Johannes-Gutenberg-University Mainz, Mainz, Germany)paper)Reference: Richter L, 2010 |
monoclonal
|
Mouse
|
ELISA, IB |
Human |
immunogen = synthetic human Ab peptide 1–16, cross-linked to keyhole limpet hemocyanin |
recognizes residues 1-16 of Amyloid β. Hybridoma cells were generated by standard procedures and screened for recognition of HSA cross-linked to Ab 1-16by ELISA; recognition of synthetic Ab 1–42 ona standardized dot blot procedure was confirmed and the epitope mapped to Ab 1–8 using a peptide array spanning the entire sequence of Ab |
- |
|
|
BAP-17(Manfred Brockhaus, F. Hoffmann-La Roche Ltd., Basel,Paper) |
monoclonal
IgG1 |
Mouse
(purified from spent hybridoma medium and conjugated to horseradish peroxidase) |
ELISA, FA |
Human |
immunogen = the peptide CMVGGVV conjugatedto keyhole limpet hemocyanin |
specific for Aβ40; no cross-reactivity observed with Ab39, Ab42 or Ab43. Ab41 showed a signal accounting for about10% of the cognate Ab40 signal |
- |
|
|
BAP-15(Manfred Brockhaus, F. Hoffmann-La Roche Ltd., Basel,Paper)Reference: Richter L, 2010 |
monoclonal
|
Mouse
(purified from spent hybridoma medium and conjugated to horseradish peroxidase) |
ELISA, FA |
Human |
immunogen = the peptide CGGVVIA conjugatedto keyhole limpet hemocyanin |
specific for Aβ42; no cross-reactivity observed with Ab39, Ab40 or Ab43. Ab41 showed a signal accounting for about10% of the cognate Ab42 signal |
- |
|
|
BAN-50(N Suzuki,Takada Chemical Industries)References:Wang S, 2010Fukumoto, 2010Suzuki, 1994 |
monoclonal
|
Mouse
|
ELISA, WB |
Human, Mouse |
raised against Aβ 1-16 (epitope at aa 1-10) |
amyloid-beta |
- |
|
|
1E8(Paul Cutler,SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Harlow, Essex, UKpaper: Cutler, et al, l997) |
monoclonal
|
Mouse
|
ELISA, IB, IH, IP |
Bovine, Human |
immunogen = the derived peptide sequence aa. 13–22, (epitope at aa. 18-22, VFFAE) of beta amyloid |
reacts with the N terminal of amyloid-beta; recognizes Aβ[1–40], Aβ[1–42] and Aβ[1–43] |
- |
|
|
Cell Signaling |
polyclonal
|
Rabbit
(liquid, protein-A purified, HEPES, NaCl, BSA, glycerol) |
FC, IF, IH, IP, WB |
Bovine, Human, Monkey, Mouse, Rat |
immunogen = synthetic peptide corresponding to residues surrounding Lys29 of human COX IV |
detects endogenous levels of total COX IV protein |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(liquid, protein-A purified, HEPES, NaCl, BSA, glycerol, azide.Also available Biotinylated, cat# 6014HRP conj., cat# 5247Alexa Fluor® 488 conj., cat# 4853) |
FC, IF, IH, IP, WB |
Bovine, Zebra fish, Pig, Human, Monkey, Rat |
immunogen = synthetic peptide corresponding to residues surrounding Lys29 of human COX IV |
detects endogenous levels of total COX IV protein |
- |
|
|
Abcam |
polyclonal
IgG |
Rabbit
(liquid, affinity purified, BSA, PBS, azide) |
IF, IH, WB |
Dog, Human, Rat |
immunogen = synthetic peptide derived from a region of human cofilin that contains serine 3; sequence is conserved in mouse and rat |
detects cofilin protein phosphorylized at Serine 3 |
- |
|
|
Cell Signaling |
monoclonal
IgG1 |
Mouse
(mono: liquid, HEPES, BSA, glycerol, azide;poly: liquid, protein-A affinity purified, HEPES, saline, BSA, glycerol, azide;) |
IF |
Bovine, Pig, Human, Mouse, Rat |
Immunogen = a synthetic phosphopeptide corresponding to residues surrounding Ser19 of human myosin light chain 2 |
detect endogenous levels of myosin light chain 2 (smooth muscle) only when phosphorylated at serine 19. These antibodies do not cross-react with the cardiac isoform of myosin light chain 2 |
- |
|
|
Cell Signaling |
monoclonal
IgG |
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide) |
IC, IF, IH, WB |
Human, Monkey, Mouse, Rat |
immunogen = a synthetic phosphopeptide corresponding to residues around Ser9 of human GSK-3β |
recognizes endogenous levels of GSK-3 only when phosphorylated at Ser9 of GSK-3β may cross weakly with the phosphorylated form of GSK-3α due to high sequence homology |
- |
|
|
BD Transduction |
monoclonal
IgG1 |
Mouse
(aqueous buffered solution, glycerol, BSA, azide) |
IF, IH, IP, WB |
Dog, Chicken, Human, Mouse, Rat |
Immunogen = rat GSK-3β, aa 1-160 |
detects GSK3β, 46 kDa |
- |
|
|
Pierce Thermo Scientific |
unknown
|
Unknown Host
|
WB |
Human |
- |
specific for HSP70-1 |
- |
|
|
A B R - Thermo Scientific |
monoclonal
|
Mouse
(liquid, protein-G affinity purified, PBS, azide) |
ELISA, FC, IC, IH, IP, WB |
Bovine, Dog, Ovine, Porcine, Rabbit, Chicken, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat, C. elegans, Drosophila |
immunogen = human Hsp70 purified from HeLa cells, corresponding to amino acids 436-503 |
specific for HSP-70; does not cross-react with Hsc70 |
- |
|
|
A B R - Thermo Scientific |
monoclonal
IgM |
Mouse
(liquid, purified, PBS, azide) |
IF, IH, WB |
Avian (various), Amphibian (various), Fish (various), Yeast, Human, Mouse, Rat, Drosophila |
immunogen = human recombinant HSP70 over expressed in E. coli |
detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70 and, following heat shock, HSP72 |
- |
|
|
A B R - Thermo Scientific |
monoclonal
IgG1 |
Mouse
(liquid, purified, PBS, azide) |
EMSA, IF, IH, IP, WB |
Avian (various), Amphibian (various), Fish (various), Yeast, Human, Mouse, Rat, Drosophila |
immunogen = human recombinant HSP70 over expressed in E. coli |
detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70 and, following heat shock, HSP72 |
- |
|
|
A B R - Thermo Scientific |
monoclonal
IgG3 |
Mouse
(liquid, protein-G affinity purified, PBS, azide) |
IC, IP, WB |
Dog, Primate (various), Human, Mouse |
immunogen = Synthetic peptide corresponding to residues G(615) S G S S G T G E Q K E D Q K E E K Q(633) of mouse mtHSP70 |
detects mitochondrial heat shock protein 70 kDa (mtHSP70) |
- |
|
|
BD Transduction |
monoclonal
IgG1 |
Mouse
(aqueous, buffered solution, BSA, azide, glycerol) |
IF, WB |
Human, Mouse, Rat |
immunogen = human Hsp60 aa. 1-200 |
detects Hsp60 |
- |
|
|
Cell Signaling |
monoclonal
IgG1 |
Mouse
(liquid, HEPES, NaCl, BSA, glycerol, azide) |
FC, IH, IP, WB |
Human, Monkey, Mouse, Rat |
Immunogen = synthetic peptide derived from the sequences of rat p44/42 MAP kinase |
Recognizes endogenous levels of total p44/42 MAP kinase (ERK1/2) protein. Does not cross-react with JNK/SAPK or p38 MAP kinase. |
- |
|
|
Cell Signaling |
monoclonal
IgG1 |
Mouse
(liquid, BSA, HEPES, NaCl, glycerol, azide.See also Cat# 4374, Alexa Fluor® 488 Conjugate;Cat# 4375, Alexa Fluor® 647 Conjugate;) |
FC, IC, IF, IP, WB |
Bovine, Mink, Zebra fish, Pig, Human, Monkey, Hamster, Mouse, Rat, Drosophila |
Immunogen = synthetic phospho-peptide corresp. to residues surrounding Thr202/Tyr204 of human p44 MAP kinase |
Detects endogenous levels pf p44/42 MAP kinase (ERK1/2) dually phosphorylated at Thr202 and tyr204. Does not cross-react with the corresp. phospho-residues of SAPK/JNK or p38 MAP kinase |
- |
|
|
Acris Antibodies |
monoclonal
IgG1 |
Rat
(liquid, supernatant, Tris/HCl, foetal calf serum, azide) |
ELISA, IH, IP, WB |
Bovine, Human |
immunogen = purified Human Articular Cartilage Aggrecan |
recognizes epitopes within the N-terminal G1-IGD-G2 region of Aggrecan, that has been identified as a cluster 1 isoform |
- |
|
|
Pierce Thermo Scientific |
polyclonal
|
Rabbit
(liquid, affinity purified, Tris-glycine, saline, azide) |
IH, WB |
Primate (various), Human, Hamster, Mouse |
Immunogen = synthetic peptide corresponding to a region within residues 1-100 of human DRP1 (16986) or residues 500-600 of human DRP1 (16987) |
detects Dynamin related protein 1 |
- |
|
|
Santa Cruz |
monoclonal
IgG1 |
Mouse
(liquid, PBS, azide, gelatin) |
ELISA, IF, IH, IP, WB |
Human, Mouse |
immunogen = amino acids 1093-1208 near the C-terminus of BAF170 of human origin |
detects BAF170 |
- |
|
|
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner |
monoclonal
|
|
IH, WB |
- |
immunogen = bovine brain Neurofilament, 150kD |
NF 150 & 200 kD, COOH terminal tail domain, axonspecific, phosphatase insensitive |
- |
|
|
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner |
monoclonal
|
|
IH, WB |
- |
immunogen = chicken brain |
150 & 200 kD, phosphatase sensitive |
- |
|
|
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner |
monoclonal
|
|
IH, WB |
- |
immunogen = bovine brain Neurofilament, 150kD |
NF 150 & 200 kD, COOH terminal tail domain, axonspecific, phosphatase insensitive |
- |
|
|
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner |
monoclonal
|
|
IH, WB |
- |
immunogen = bovine brain Neurofilament, 150kD |
NF 150 & 200 kD, COOH terminal tail domain, axonspecific, phosphatase insensitive |
- |
|
|
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner |
monoclonal
|
|
IH, WB |
- |
immunogen = bovine brain Neurofilament, 150kD |
NF 150 & 200 kD, COOH terminal tail domain, axonspecific |
- |
|
|
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner |
monoclonal
|
|
IH, WB |
- |
immunogen = bovine brain Neurofilament, 150kD |
NF 150 & 200 kD, COOH terminal tail domain, axon& perikarya |
- |
|
|
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner |
monoclonal
|
|
IH, WB |
- |
immunogen = bovine brain Neurofilament, 150kD |
NF 150 & 200 kD, COOH terminal tail domain, axon& perikarya |
- |
|
|
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner |
monoclonal
|
|
IH, WB |
- |
immunogen = bovine brain Neurofilament, 150kD |
NF 150 & 200 kD, COOH terminal tail domain, axonspecific, phosphatase insensitive |
- |
|
|
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner |
monoclonal
|
|
IH, WB |
- |
immunogen = chicken brain Neurofilament |
NF 150 kD and GFAP, amino terminal rod, phosphatase insensitive,does not stain astrocytes |
- |
|
|
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner |
monoclonal
|
|
IH, WB |
- |
immunogen = chicken brain |
NF 200 kD, axon specific, phosphatase sensitive |
- |
|
|
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner |
monoclonal
|
|
IH, WB |
- |
immunogen = bovine brain Neurofilament, 200kD |
NF 200 kD, COOH terminal tail domain, phosphatase sensitive |
- |
|
|
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner |
monoclonal
|
|
IH, WB |
- |
immunogen = bovine brain Neurofilament, 200kD |
NF 200 kD, cell bodies positive, phosphatase insensitive |
- |
|
|
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner |
monoclonal
|
|
IH, WB |
- |
immunogen = bovine brain Neurofilament, 200kD |
NF 200 kD, cell bodies positive, phosphatase insensitive |
- |
|
|
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner |
monoclonal
|
|
IH, WB |
- |
immunogen = bovine brain Neurofilament, 200kD |
NF 200 kD, phosphatase sensitive |
- |
|
|
RMO24(Virginia Lee, U of Penn, CNDR, Philadelphia, PA)used in paper by Giasson, 2002 |
monoclonal
|
|
- |
- |
|
phosphorylated NF heavy subunit |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Rabbit
(liquid, PBS, azide, gelatin) |
ELISA, IF, IH, IP, WB |
Human, Mouse, Rat |
immunogen = peptide corresp. to amino acids 1-145 representing full length Tom20 of human origin |
recognizes Tom20 |
- |
|
|
Santa Cruz |
polyclonal
IgG |
Goat
(liquid, affinity purified, PBS, azide, gelatin) |
ELISA, IF, WB |
Human, Mouse |
immunogen = apeptide mapping within an internal region of OPA1 (dynamin family) of human origin. |
for detection of Optic atrophy 1 gene protein |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
IgG1 |
Rabbit
(liquid, raw rabbit serum) |
WB |
Human, Mouse, Rat |
- |
recognizes mitofilin, mitochondrion inner membrane protein |
- |
|
|
BD Transduction |
monoclonal
IgG1,κ |
Mouse
(aquaeous buffered solution, BSA, glycerol, azide, FITC conj.) |
IF |
Dog, Human, Mouse, Rat |
immunogen = Rat GM130, aa. 869-982 |
recognizes GM130, 130 kDa |
- |
|
|
R&D Systems |
monoclonal
IgG2a |
Mouse
(filtered solution, protein-G affinity purified, PBS, trehalose, lyophilized) |
IH, WB |
Human |
immunogen = purified, E. coli-derived, recombinant human High Temperature Requirement Protein-2 (rhHtrA2; aa 134-458) |
detects the mitochondria-processed form of endogenous human HtrA2/Omi. |
- |
|
|
BD Transduction |
monoclonal
IgG2a |
Mouse
(Aqueous buffered solution, affinity purified, BSA, glycrol, azide) |
IF, WB |
Human, Mouse, Rat |
immunogen = rat Tim23 aa. 5-126. |
detects Tim23, 23 kDa by WB |
- |
|
|
Sigma Aldrich |
polyclonal
|
Rabbit
(buffered aqueous solution, affinity isolated, PBS, azide) |
IB, IF |
Mouse, Rat |
immunogen = a synthetic peptide corresponding to amino acid residues 404-415 of mouse LAMP2 with N-terminal added cysteine, conjugated to KLH |
detects LAMP-2 |
- |
|
|
R&D Systems |
polyclonal
IgG |
Rabbit
(filtered solution, affinity purified, PBS, trehalose, lyophilized) |
IC, WB |
Human, Mouse |
immunogen = E. coliderived recombinant human HTRA2/Omi Ala134Glu45 |
detects full length and mitochondria processed HTRA2/Omi |
- |
|
|
BD Transduction |
monoclonal
IgG1,κ |
Mouse
(aquaeous buffered solution, BSA, glycerol, azide, affinity purified) |
IF, WB |
Dog, Human, Mouse, Rat |
immunogen = Rat GM130, aa. 869-982 |
recognizes GM130, 130 kDa |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG1 |
Mouse
(liquid, PBS, glycrol, azide, Protein G purified) |
IP, WB |
Human |
Immunogen = peptide corresp. to aa 551-599 of human Sirt1 |
SIRT1(120kDa by WB). An additional unidentified band was detected (100 kDa). |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG |
Rabbit
(liquid, Tris-Glycine, saline, BSA, glycrol, azide) |
IC, IH, IP, WB |
Human |
Immunogen = synthetic peptide corresponding to residues near C-terminus and before Ala-rich domain of human Sir2 |
recognizes SIRT1(~110 kDa by WB); does not cross-react with other sirtuin family members. |
- |
|
|
Chemicon and Upstate are Millipore |
monoclonal
IgG,κ |
Mouse
(liquid, purified, Tris-Glycine, saline, azide) |
IC, IH, IP, WB |
Human, Mouse |
Immunogen = His-tagged recombinant protein corresponding to human SIRT1; mouse sequence is 100% identical to immunogen |
recognizes SIRT1 |
- |
|
|
Chemicon and Upstate are Millipore |
polyclonal
IgG |
Rabbit
(liquid, purified, Tris-Glycine, saline, azide, glycerol) |
IC, IP, WB |
Human, Mouse |
Immunogen = His-tagged fusion protein corresponding to amino acids 1-131 of mouse Sir2α |
recognizes SIRT1, ~110 kDa |
- |
|
|
Ab708Li Gan, Gladstone Institute of Neurological DiseaseAssistant Professor of NeurologyUniversity of California, San FranciscoReference: Min SW, 2010 |
polyclonal
IgG2b |
Rabbit
|
WB |
Human, Rat |
immunogen = a synthetic tau peptide (amino acids 160–182 for 2N4R tau isoform) containing acetylated lysines at positions 163 and 174 and 180 |
Ab708 specifically recognizes tau acetylated by p300 under cell-free conditions. In HEK293T cells transfected with tau, overexpression of p300 markedly elevatedthe levels of ac-tau detected with Ab708 while the increase in the levels of t-tau was modest, suggesting that Ab708 preferentially recognizes p300-induced ac-tau in cultured cells. Ab708 recognizes human tau acetylated at positions 163, 174, or 180 and possibly other acetylated lysines on tau, but not nonacetylated tau |
- |
|
|
9ABLi Gan, Gladstone Institute of Neurological DiseaseAssistant Professor of NeurologyUniversity of California, San FranciscoReference: Min SW, 2010 |
polyclonal
|
Rabbit
|
WB |
Human, Mouse |
immunogen = the microtubule-binding region aa 264–287 (ENLKHQPGGG VQIINKLDLSNV), which is 100% conserved between mouse and human |
9AB recognizes recombinant tau acetylated by GST-p300, but not tau incubated with GST alone, suggesting that 9AB does not cross-react with non-ac-tau. 9AB also preferentially recognizes p300-induced ac-tau in cultured cells. In mouse brains, 9AB detected low levels of ac-tau. Does not recognize nonacetylated recombinant tau |
- |
|
|
Peter Davies, Department of Pathology, Albert Einstein College of MedicineReference: Zempel H, 2010 |
monoclonal
IgG1 |
Mouse
|
IH |
Human, Mouse |
epitope at aa102–140 |
recognizes total tau |
- |
|
|
Abcam |
monoclonal
IgG1 |
Mouse
(liquid, IgG fraction, PBS, azide.For BSA and azide free, see ab80579) |
IH, WB |
Human |
immunogen = purified bovine microtubule associated proteins |
specific to 45-68 kDa proteins identified as tau proteins; no cross with tubulin or other MAPs; phospho. as well as non-phospho. forms of tau |
- |
|
|
Biosource Invitrogen |
polyclonal
|
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated) |
IH, WB |
Human |
human tau-derived peptide that is phosphorylated at threonine 205 |
tau phosphorylated at threonine 205 |
- |
|
|
Biosource Invitrogen |
polyclonal
|
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated) |
WB |
Human |
human tau-derived peptide that is phosphorylated at serine 422 |
tau phosphorylated at serine 422 |
- |
|
|
Pierce Thermo Scientific |
monoclonal
IgG1κ |
Mouse
(supernatant, affinity purified, no carrier or preservativeAlso available biotin conj., MN-1050B) |
IH, WB |
Human |
immunogen == partially purified human PHF tau, aa. around phosphorylated Thr181 |
PHF tau, tamgles and neurofilaments; no cross with recomb. unphospho tau; weak cross with normal tau |
- |
|
|
Dako |
polyclonal
Ig |
Rabbit
(serum, purified Ig fraction, azide) |
IC, IH, IP, WB |
Human, Mouse, Rat |
immunogen = C-terminal part of human tau protein expressed in E. coli; aa. 243-441, contains the four repeated sequences involved in microtubule binding (MTB) |
reacts with tau protein and with tau from Alzheimer paired helical filaments; also MAP2; well-suited for gel immunoprecipitation techniques |
- |
|
|
Biosource Invitrogen |
polyclonal
|
Rabbit
(affinity purified Ig, Dulbecco's PBS, BSA, glycerol, unconjugated) |
WB |
Human |
phospho peptide derived from the region of human Tau that contains serine 214 |
detects tau phosphorylated at serine 204; no cross with non phosphorylated tau |
- |
|
|
Clontech, a Takara Bio company |
monoclonal
IgG2a |
Mouse
(liquid, affinity purified, PBS, filter-sterilized) |
IC, IP, WB |
Mammal (various), Microorganism (various) |
immunogen = Aequorea victoria green fluorescentprotein (EGFP) |
recognizes native and denatured forms of wild-type GFP, GFPuv, EGFP, destabilized EGFP variants, EBFP, EYFP, ECFP, AcGFP, and both N- and C-terminal fusion proteins containing these GFP variants in bacterial and mammalian cell lysates |
- |
|
|
Clontech, a Takara Bio company |
monoclonal
IgG1,κ |
Mouse
(liquid, affinity purified, Tris-HCl, azide) |
IC, WB |
Mammal (various), Microorganism (various) |
immunogen = recomb. full-lengthAequorea victoria green fluorescentprotein (rGFP) |
reacts with wild-type GFP, GFPuv, EGFP, destablizedEGFP variants, EBFP, EYFP, ECFP andboth N- and C-terminal fusion proteinscontaining these GFP variants |
- |
|
|
R&D Systems |
monoclonal
IgG2A |
Mouse
(supernate, protein-A or G-purified, PBS, trehalose, filtered, lyophilized) |
IC, WB |
Mammal (various), Chicken |
immunogen = microtubules derived from rat brain |
neuron specific class III β tubulin; not other β-tubulin in glial cells. Stains neuronal cell bodies, dendrites, axons and axonal terminations |
- |
|
|
R&D Systems |
monoclonal
IgG2A |
Mouse
(Protein A or G purified from hybridoma culture supernatant, PBS, BSA, biotinylated, lyophilized) |
IC, WB |
Mammal (various), Chicken |
immunogen = microtubules derived from rat brain |
detects mammalian and chicken neuronspecific βIII tubulin but not other βtubulin isotypes |
- |