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Antibodies

29333 Results

Name Source Clonality &
Isotype
Host &
Formulation
Method Reactivity Immunogen/
Epitope
Specificity Availability
APP, APLP, AICD From labs (6)
anti-APP 369(Sam Gandy,Mount Sinai Alzheimer's Disease Research Center, NYC)References:Lane RF, 2010Vieira SI, 2010Pavlov PF, 2010Li H, 2010Gandy, 2010Zhang, 2009Liu, Y, 2009Hoe, 2009Tampellini, 2007White, 2006Puglielli, 2001Li, 2003 polyclonal
Rabbit
IH, IP Human, Mouse immunogen = entire cytoplasmic tail of APP(paper) C-terminal APP -
anti-APP C7(Dr. Dennis Selkoe,Harvard Univ. Medical School, Boston, MA)Reference: Puglielli, 2001,by Yu, 2001 polyclonal
Rabbit
IH - last 20 residues of APP   -
anti-APP C9(Dr. Dennis Selkoe,Harvard Univ. Medical School, Boston, MA)References:Muresan, 2009Sato, 2007 polyclonal
Rabbit
IP, WB - synthetic peptide corresp. to APP732-751, (APP751 numbering)   -
anti-APP α-1(Dr. Dennis Selkoe,Harvard Univ. Medical School, Boston, MA polyclonal
Rabbit
IH, WB Human synthetic peptide of residues 649-664 APP -
R1736(Dr. Dennis Selkoe,Harvard Univ. Medical School, Boston, MA)Reference: Maier, 2008, polyclonal
Rabbit
(antiserum)
IP, WB Rodent (various), Human immunogen = synthetic peptide of amino acids 595–611 of APP APP epitope corresponds to positions -2 to +15 of human Abeta, and it presents 90%(15 out of 17 amino acids) of sequence homology with the hamster sequence which is identical to other rodents such as mouse -
anti-APP C8(Dr. Dennis Selkoe,Harvard Univ. Medical School, Boston, MA)References: Davis, 2010Senechal, 2008Wang, 2007 polyclonal
Rabbit
DB, IC, IH, WB Mammal (various), Human residues 676-695, comprising the 20 C-terminal amino acids of human βAPP cytoplasmic domain.Paper, Selkoe et al. recognizes full length APP and all CTFs -
APP, APLP, AICD From manufacturers (3)
Biosource Invitrogen polyclonal
Goat
(liquid, whole serum, azide, unconj.)
ELISA, WB Human immunogen = synthetic peptide (G Y E N P T Y K F F E Q M Q N) corresp. to aa sequence 681-695 of C terminus of human APP C-terminus of APP(100kDa), may cross-react with APLP2 -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, PBS, azide, purified)
IF, IH, WB Fish (various), Dog, Pig, Human, Monkey, Mouse, Rat immunogen = purified rhN-terminal epitope (aa 66-81) on the pre-A4 molecule, fusion protein human pre-A4 APP; recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa; cross reacts with APLP2 -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(lyophilized, ascites)
IH, WB Human, Rat immunogen = C-terminal fragment APP643-695/Jonas full-length APP and selectively with the cytoplasmic C-terminal fragment of APP 643-695 -
Actin Related From manufacturers (1)
Sigma Aldrich monoclonal
IgG1
Mouse
(aqueous solution, purified, PBS, azide)
EM, IB, IC, IH Bovine, Cat, Dog, Chicken, Carp, Sheep, Pig, Human, Guinea Pig, Mouse, Rat, Drosophila immunogen = slightly modified β-cytoplasmic actin N-terminal peptide, Ac-D D D I A A L V I D N G S G L, KLH conj. detects 42 kDa beta-actin -
Amyloid-β From labs (1)
anti-amyloid beta 21F12(Frederique Bard,Elan Pharmaceuticals, So. San Francisco, CA Paper: Johnson-Wood, et al, PNAS February 18, 1997)References:Siderowf, 2010Okereke, 2009Serneels, 2009Brody, 2008Holmes, 2008Shankar, 2008Roberson, 2007Salehi, 2006 monoclonal
Unknown Host
IH, WB - immunogen = synthetic peptide fragment derived from different regions of amyloid-beta and coupled to a carrier protein amyloid-beta; recognizes amino acids 33-42; negligible crossreactivity (1–40 -
Amyloid-β From manufacturers (3)
Innogenetics kit
Unknown Host
(well standardized multiparameter bead-based immunoassay for the simultaneous quantification of human ß-amyloid in plasma using xMAP® (registered trademark of Luminex Corp)technology)
ELISA Human Uses 3D6 antibody;3D6 selectively binds Aß peptides with a free aminogroup at Asp1 (epitope 1–5) for simultaneous quantification of different forms of β-amyloid in blood, with check for matrix interference -
Synaptic Systems monoclonal
IgG2b
Mouse
(affinity purified, buffered, azide, lyophilized)
IH, WB Human, Mouse, Rat Balb/c mice were immunized with a KLH-coupled pyroglutamate-Abeta-peptide with the peptide pE F R H D-C.After preparation of the lymph nodes, cells were fused with the myeloma cell line P3-963-Ag8 reactive to N-terminal truncated Abeta 3-X starting with pyroglutamate (pGlu, pE). Does not cross-react with N-terminal truncated Abeta 11-X starting with pGlu. -
Synaptic Systems monoclonal
IgG1
Mouse
(affinity purified, buffered, azide, lyophilized)
IH, WB Human, Mouse, Rat Balb/c mice were immunized with a KLH-coupled pyroglutamate-Abeta-peptide with the peptide pEFRHD-C.After preparation of the lymph nodes, cells were fused with the myeloma cell line P3-963-Ag8 reactive to N-terminal truncated Abeta 3-X starting with pyroglutamate (pGlu, pE). Does not cross-react with N-terminal truncated Abeta 11-X starting with pGlu. -
Astrocyte Markers From manufacturers (1)
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(ascites, azide)
IC, IH, WB Bovine, Rabbit, Chicken, Pig, Human, Mouse, Rat purified GFAP from porcine spinal cord. recognizes ~50 kDa band; astrocytes and Bergmann glia cells, glioma and glial cell derived tumors -
CD11b, Integrins From manufacturers (6)
Chemicon and Upstate are Millipore monoclonal
IgG2b
Rat
(liquid, purified, PBS, azideAlso available Phycoerythrin conjugated, CBL1313P)
FC, IF, IH, IP Mouse immunogn = B10 mouse spleen cells enriched with T cells specific to CD11b; recognizes a differentiation antigen situated on mononuclear phagocytes and granulocytes; the expression of this antigen (Mac 1) increases during monocyte maturation -
Chemicon and Upstate are Millipore monoclonal
IgG2a
Mouse
(liquid, protein-A affinity purified, PBS, azide.Also available protein-G purified, PBS, no preservatives, CBL1512Z, or FITC conjugated, CBL1512F)
FC, IH, IP Rat immunogn = rat peritoneal macrophages recognizes most macrophages (including resident peritoneal and activated macrophages), Kupffer cells, but only about 35% of alveolar macrophages; also labels dendritic cells extensively, granulocytes and cells with the morphology of microglia in brain -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, purified, PBS, BSA, azide.Also available Phycoerythrin conjugated, CBL145P)
FC, IH, IP, IS Baboon, Human, Monkey immunogn = synovial cells and human monocytes reacts with the integrin heterodimer of 165 kDa which comprises the CD11b receptor -
Chemicon and Upstate are Millipore monoclonal
IgG2b
Rat
(liquid, protein-G purified, PBS, no preservatives)
FC, IF, IH, IP, IS Human, Mouse immunogn = T cell enriched splenocytes from B10 mice recognizes the murine alphaM integrin chain; stains polymorphonuclear cells, monocytes and NK cells -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, ascites, protein-A affinity purified, PBS, azide.)
IC, IH Rat immunogn = spleen cell homogenate with FCA recognizes a membrane antigen on rat macrophages, monocytes, dendritic cells and granulocytes; recognizes small ramified (micro) glial cells in the central nervous system -
Chemicon and Upstate are Millipore monoclonal
IgG2a
Mouse
(liquid, purified, PBS, azide)
FC, IC, IH, IP, IS Human immunogn = leukemic cells of CML specific to CD11b; reacts with the CR3-receptor of inactivated C3b component of complement (C3bi) -
CD_ except CD11b, CD40, CD45 and CD147 From manufacturers (1)
AbD Serotec monoclonal
IgG2a
Rat
(liquid, protein-G affinity purified IgG from supernatant, PBS, azide)
FC, IH, IP, WB Mouse - recognizes the murinehomologue of the human macrophage glycoprotein CD68 -
CREB, cAMP, CBP From manufacturers (1)
Cell Signaling monoclonal
IgG
Rabbit
(liquid, Hepes, NaCl, BSA, glycerol, Protein A and affinity purified)
FC, IC, IF, IH, IP, WB Human, Monkey, Mouse, Rat immunogen = synthetic phospho-peptide (KLH-coupled) corresp. to residues surrounding Thr71 of human ATF-2 (CRE-BP1) reacts with endogenous levels of ATF-2 only when phosphorylated at threonine 71; no cross-reaction with phosphorylated c-Jun, CREB or other transcription factors; reacts with both Thr69/Thr71 dually phosphorylated ATF-2 and Thr71 singly phosphorylated ATF-2 equally well -
Caspases From manufacturers (1)
Cell Signaling polyclonal
IgG
Rabbit
(liquid, protein-A, affinity purified)
FC, IC, IF, IH, WB Bovine, Human, Monkey, Mouse, Rat immunogen = synthetic peptide (KLH-coupled) corresp. to amino-terminal residues adjacent to Asp175 of human caspase-3 detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175; does not recognize full length caspase-3 or other cleaved caspases -
Cell Cycle From manufacturers (4)
Upstate and Chemicon are Millipore polyclonal
IgG
Rabbit
(liquid, antiserum)
IP, WB Avian (various), Human, Mouse, Rat immunogen = synthetic peptide corresponding to aa 35-51 (TPQHYPSFG VTSIPNYN) of human Fyn detects Fyn p59 -
Cell Signaling polyclonal
Rabbit
(liquid, protein A and peptide affinity purified, HEPES, saline, BSA, glycerol)
WB Chicken, Human, Mouse, Rat, Xenopus immunogen = synthetic phosphopeptide corresponding to residues surrounding Tyr416 of human Src detects endogenous levels of Src only when phosphorylated at Tyr416; antibody may cross-react with other Src family members such as Yes, Fyn, Fgr and Yrk when phosphorylated at the equivalent sites -
Cell Signaling monoclonal
IgG
Rabbit
(liquid, protein A and peptide affinity purified, HEPES, saline, BSA, glycerol, azide)
WB Human, Mouse, Rat immunogen = synthetic phosphopeptide corresponding to residues surrounding Tyr416 of human Src detects endogenous levels of Src only when phosphorylated at Tyr416; antibody may cross-react with other Src family members such as Yes, Fyn, Fgr and Yrk when phosphorylated at the equivalent sites -
Upstate and Chemicon are Millipore monoclonal
IgG
Mouse
(frozen liquid, protein G purified, Tris-glycine, saline, azide)
IB, IP Bovine, Human immunogen = recombinant human p35 recognizes 33 kDa p35 -
G-protein Related From manufacturers (1)
Synaptic Systems monoclonal
IgG1
Mouse
(purified IgG, lyophilized)
IC, IP, WB Cow, Human, Hamster, Mouse, Rat immunogen = purified Rab-GDI of bovine brain recognizes Rab-GDI-α and -β; immunoprecipitates Rab-GDI complexes -
GABA & Related From manufacturers (2)
Synaptic Systems monoclonal
IgG1
Mouse
(purified IgG, lyophilized)
ELISA, IC, IH, IP, WB Human, Mouse, Rat immunogen = purified recombinant doublet of E-Domain (aa 307 - 735) (epitope: C6 domain located in the first half of the E-domain) detects all splice variants that contain a complete E-domain including the C6 domain -
Synaptic Systems polyclonal
Rabbit
(affinity purified with the immunogen, lyophilized)
IP Human, Monkey, Mouse, Rat Immunogen = synthetic peptide AEPPVEGDIHYQR (aa residues 75 - 87 in rat) coupled to KLH Specific for vGAT -
GSK-3α & GSK-3β From manufacturers (2)
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified;also available as rabbit, sc-11757-R)
IF, IH, WB Fish (various), Zebra, Human, Mouse, Rat, Xenopus Immunogen = short amino acid sequence containing phos-phorylated Ser9 of human GSK-3β Ser9 phosphorylated GSK-3β -
Chemicon and Upstate are Millipore monoclonal
IgG
Rabbit
(liquid, Tris-glycine, saline, gl;ycerol, azide, BSA)
IC, IH, IP, WB Human Immunogen = phospho-specific peptide corresponding to residues surrounding Ser9 of human GSK3 β detects only GSK3 β phosphorylated on Ser9 -
Glutamate Receptor & Related From manufacturers (5)
Phospho- solutions polyclonal
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified)
WB Human, Mouse, Rat Immunogen = phosphopeptide corresp. to aa residues surrounding the anti-phospho-Try1472 of NMDA Receptor NR2B ~180 NMDA Receptor NR2B-subunit phosphorylated at Tyr1472, also labels ~65k and ~115k. -
Chemicon and Upstate are Millipore polyclonal
IgG
Rabbit
(liquid, tris-glycine saline, azide, Protein G purified)
IC, IP, WB Bovine, Dog, Human, Monkey, Mouse, Rat Immunogen = peptide comprising the C-terminal 20 aa of mouse NR2B (K F N G S S N G H V Y E K L S S I E S D V) NR2B -
Chemicon and Upstate are Millipore monoclonal
IgG
Mouse
(liquid, purified IgG, Tris-glycine, saline, azide)
IC, IP, WB Human, Mouse, Rat Immunogen = His-tag fusion protein corresponding to residues 834-938 of rat NR1A; epitope maps to a region (a.a. 834-864) common to all NR1 isoforms detects NMDAR1, 130 kDa by WB -
Chemicon and Upstate are Millipore polyclonal
Rabbit
(liquid, unpurified serum, azide)
WB Mouse, Rat immunogen = GST-tagged recombinant protein corresponding to rat NR2A at the C-terminus recognizes NR2A (~176 kDa) at the C-terminus -
Chemicon and Upstate are Millipore polyclonal
IgG
Rabbit
(liquid, tris-glycine saline, glycerol, azide, Protein A purified)
IP, WB Mouse, Rat immunogen = 6His-tagged fusion protein corresp.to aa1265-1464 of mouse NMDA receptor 2A (NR2A). NR2A (170 kDa) -
Heat Shock Proteins From manufacturers (3)
Synaptic Systems monoclonal
IgG1
Mouse
(purified IgG, lyophilized)
IC, WB Cow, Human, Hamster, Mouse, Rat immunogen = purified bovine ATPase (epitope between aa 391-546) detects Hsp70, specific for uncoating ATPase -
Pierce Thermo Scientific monoclonal
IgG2a
Rat
(liquid, Protein G purified, PBS)
ELISA, FC, IC, IH, IP, WB Bovine, Dog, Ovine, Porcine, Rabbit, Chicken, Various species, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat immunogn = full length, native Hsc70 protein purified from sodium arsenite treated heat-resistant variants of Chinese hamster cells specific for Hsc70 -
Stressgen Enzo Life Sciences monoclonal
IgG2a
Rat
(liquid, PBS, azide, glycerol, affinity purified)
ELISA, FC, IC, IF, IH, IP, WB Bovine, Dog, Chicken, Turtle, Sheep, Pig, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat immunogen = native hamster Hsc70 (Hsp73) recognizes ~73 kDa Hsc70 -
Iron Related From manufacturers (1)
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide, glycerol)
ELISA, IH, WB Human immunogen = synthetic peptide corresponding to N-terminal residues of human MTP1 (Metal transporter protein 1), conj. to KLH detects ferroportin -
Kinases From manufacturers (2)
Abcam polyclonal
IgG
Rabbit
(liquid, affinity purified, glycerol, BSA, PBS, azide)
IC, IF, IH, WB Human, Mouse immunogen = Synthetic peptide (Human) derived from the region of JNK 1 + 2 that contains threonine 183 and tyrosine 185, based on the human sequence (conservd among many species) detects JNK 1 + 2 phosphorylated at threonine 183 and tyrosine 185 -
BD Transduction monoclonal
IgG1
Mouse
(liquid, affinity purified)
IF, WB Dog, Frog, Human, Mouse, Rat Immunogen = aa 353 to 486 from rat CASK detects CASK -
MAPKs From manufacturers (3)
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide, afffinity purified)
FC, IF, IH, IP, WB Chicken, Zebra fish, Human, Mouse, Rat, Xenopus Immunogen = peptide mapping at the C-terminus of rat ERK 2 detects ERK 2 p42, and to a lesser extent, ERK 1 p44 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide, available as phycoerythrin conjugate sc-153 PE)
FC, IF, IP, WB Chicken, Zebra fish, Human, Mouse, Rat, Xenopus, Drosophila Immunogen = peptide mapping subdomain XI (r) of rat ERK 2 detectss ERK 2 p42, and to a lesser extent, ERK1 p44 -
Santa Cruz monoclonal
IgG2a
Mouse
(liquid, PBS, gelatin, azide, available conj. phycoerythrin sc-7838 PE, agarose sc-7838 AC, fluorescein sc-7383 FITC or rhodamine sc-7383 TRITC)
IF, IH, IP, WB Human, Mouse, Rat Epitope corresponding to a sequence containing phosphorylated Tyr-204 of human ERK ERK1 and ERK2 phosphorylated at Tyr-204 -
MAPs From manufacturers (2)
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(purified IgG, phosphate buffer, saline, azide.Also available as AlexaFluor® 488 conjugate)
IH, WB Bovine, Chicken, Quail, Human, Mouse, Rat, Xenopus immunogen = bovine brain microtubule protein MAP2 in neuronal cell bodies and dendrites -
Chemicon and Upstate are Millipore polyclonal
Rabbit
(liquid, purified, PBS, azideAlso availanle ready to use, Cat# IHCR1004-6)
ELISA, IC, IH, WB Human, Mouse, Rat immunogen = purified rat brain MAP MAP2a and MAP2b and (lesser) MAP2c and MAP2d -
Microglial Markers From manufacturers (2)
Wako Chemicals polyclonal
Rabbit
(sera, affinity purified, TBS, no preservatives or stabilizers)
WB Human, Mouse, Rat Immunogen = synthetic peptide corresponding to the C-terminus of Iba-1 (Ionized calcium binding adaptor molecule 1) Microglia and macrophages, but no cross reactivity with neurons and astrocytes -
Wako Chemicals polyclonal
Rabbit
(sera, affinity purified, TBS, no preservatives or stabilizers)
IC Human, Mouse, Rat Immunogen = synthetic peptide corresponding to the C-terminus of Iba-1 (Ionized calcium binding adaptor molecule 1) Microglia and macrophages, but no cross reactivity with neurons or astrocytes -
Motor Proteins From manufacturers (1)
Chemicon and Upstate are Millipore monoclonal
IgG2b
Mouse
(liquid, phosphate buffer, saline, azide)
ELISA, IC, IH, IS, RIA, WB Mammal (various), Chicken, Sea Urchin, Squid, Human, Mouse, Rat, Clam immunogen = bovine brain kinesin, epitope at aa 420-445 Reacts with the heavy chain of kinesin near the N-terminus -
Neurofilaments From manufacturers (1)
Cell Signaling monoclonal
IgG1
Mouse
(liquid, purified Ig, azide)
IF, IH, IP, WB Human, Mouse, Rat immunogen = rat neurofilament detects endogenous levels of total Neurofilament-H protein, 180-220 kDa -
Neuronal Markers From manufacturers (1)
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, purified Ig, phosphate buffer, NaCl, azideAlso available prediluted, IHR1001-6)
IC, IF, IH, IP, WB Avian (various), Primate (various), Chicken, Salamander, Ferret, Pig, Human, Mouse, Rat immunogen = purified cell nuclei from mouse brain reacts with most neuronal cell types; is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells -
Nitric Oxide From manufacturers (3)
BD Transduction monoclonal
IgG2a
Mouse
(aqueous buffered solution containing BSA, glycerol, azide, affinity purified, FITC conj.)
IF Mouse immunogen = mouse iNOS aa. 961-1144 recognizes ~130 kDa iNOS -
BD Transduction monoclonal
IgG1
Mouse
(aqueous buffered solution containing BSA, glycerol, azide)
IF, IP, WB Mouse immunogen = mouse iNOS aa. 961-1144 at C-terminal recognizes ~130 kDa iNOS; reported to cross-react with nNOS and eNOS -
BD Transduction monoclonal
IgG2a
Mouse
(aqueous buffered solution containing BSA, glycerol, azide)
IF, IP, WB Mouse immunogen = mouse iNOS aa. 961-1144 recognizes ~130 kDa iNOS -
Organelles From manufacturers (2)
BD Transduction monoclonal
IgG1
Mouse
(aquaeous buffered solution, BSA, glycerol, azide, affinity purified)
IF, WB Dog, Human, Mouse, Rat immunogen = Rat GM130, aa. 869-982 recognizes GM130, 130 kDa -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin.Also available as sc-18822 PE or sc-18822 FITC conjugates for FC, and Alexa Fluor® for FC or IF)
FC, IC, IP, WB Human immunogen = adherentspleen cells of human origin detects LAMP-2 -
PSD95 From manufacturers (1)
Pierce Thermo Scientific monoclonal
IgG2a
Mouse
(liquid, purified, PBS, azide)
IC, IF, IH, IP, WB Mouse, Rat, Xenopus immunogen = purified recombinant rat PSD-95 detects ~95 kDa PSD protein -
Phosphatases From manufacturers (2)
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
IF, IP, WB Mouse, Rat immunogen = an 18 amino acid sequence mapping at the N-terminus of STEP46 of rat origin for detection of all known isoforms of STEP of mouse and rat origin -
Cell Signaling monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
IF, IH, IP, WB Human, Monkey, Mouse, Rat, Drosophila immunogen = a synthetic peptide corresp. to the sequence of human PP2A C subunit protein detects endogenous levels of PP2A catalytic subunit protein (both alpha and beta isoforms); does not cross-react with other PP2A subunits -
Protein Trafficking From manufacturers (3)
Abcam polyclonal
IgG
Goat
(liquid, affinity purified, Tris, BSA, azide)
IF, WB Human immunogen = synthetic peptide: S P E S E G P I Y E G L I L, corresp. to C terminal aa 783-796 of Human Vps35 detects Vps35 (~90 kDa, WB) -
Abcam polyclonal
IgG
Rabbit
(liquid, affinity purified, Thimerosal, glycerol, Tris glycine)
WB Human immunogen = synthetic peptide corresponding to a region within amino acids 733-796 of human VPS35 detects Vps35 -
Abcam monoclonal
IgG2a, κ
Mouse
(liquid, PBS, no preservative, protein G purified)
WB Human immunogen = recombinant fragment corresp. to aa 697-797 of human VPS35 Vps35 -
SOD From labs (1)
C4F6Makoto Urushitani, Department of Anatomy and Physiology, Laval University Research Centre, Centre Hospitalier de l'Université Laval, Québec, Canada PaperReference: Bosco DA, 2010 monoclonal
Mouse
(liquid)
ELISA, WB Human, Mouse Immunogen = recombinant G93A SOD1 protein recognizes the G93A SOD1 and to a lower extent other mutant SOD1 forms, including G37R and G85R; very poor reactivity to the wild type SOD1 -
SOD From manufacturers (3)
the Binding Site polyclonal
Sheep
(liquid, Glycine Buffered Saline (GBS), EACA, EDTA, bnzamidine, azide)
WB Human Immunogen = human SOD Cu/Zn, Purified from Erythrocytes reacts with human SOD Cu/Zn -
Calbiochem EMD Biosciences polyclonal
IgG
Sheep
(liquid, glycine-buffered saline, EDTA, azide, e-amino-n-caproic acid, benzamidine)
IH, WB Human, Monkey, Mouse, Rat Immunogen = highly purified superoxide dismutase (SOD) Cu/Zn enzyme from human erythrocytes Recognizes Cu/Zn SOD in red blood cells. Does not cross-react with Mn SOD. -
Sigma Aldrich monoclonal
IgG1
Mouse
(liquid, ascites, azide)
ELISA Dog, Human, Rat immunogen = recombinant human Cu-Zn SOD recognizes natural and recombinant CuZn SOD -
Sortilins From manufacturers (2)
BD Transduction monoclonal
IgG2a
Mouse
(Aqueous buffered solution, BSA, glycerol, azide, affinity purifiedSee also Cat# 612663 for IH)
WB Human, Mouse, Rat Immunogen = human LR11 (aa. 1220-1337) detects LR11; 250 kDa by WB -
BD Transduction monoclonal
IgG2a
Mouse
(Aqueous buffered solution, BSA, glycerol, azide, affinity purified)
IH, WB Human, Mouse, Rat, Drosophila Immunogen = human LR11 (aa. 1220-1337) detects LR11; detects a band of ~250 kDa in S2 cells and a 250 kDa plus a 200 kDa in rat cerebrum and Drosophila embryos -
Synaptic Vesicle Fusion From manufacturers (8)
Synaptic Systems monoclonal
IgG1
Mouse
(purified IgG or ascites, lyophilized)
IC, IH, IP, WB Various vertebrate, Human, Mouse, Rat immunogen = recombinant full length rat SNAP 25 (epitope in the conserved N-terminal portion of the protein, aa 20-40 ) specific for SNAP 25; recognizes splice variants SNAP 25A and B, and the Botulinum neurotoxin A and E cleavage products -
Synaptic Systems monoclonal
IgG1
Mouse
(lyophilized, purified IgG)
IH, IP, WB Human, Hamster, Mouse, Rat immunogen = synthetic peptide (SATAATVP PAAPAGEG) corresponding to residues 2-17 of rat Synaptobrevin 2, coupled to KLH specific for Synaptobrevin2/ VAMP2; no cross with VAMP1 or VAMP3 -
Synaptic Systems monoclonal
IgG1
Mouse
(lyophilized, purified IgGAlso available as fluorescence labeled, 101011C3 and 101011C5,or biotin labeled, 101011BT)
IC, IH, IP, WB Mammal (various), Human, Mouse, Rat immunogen = electro phoretically purified synaptophysin, (epitope in cytoplasmic tail) Detects synaptophysin 1 with no cross-reactivity towards other synaptophysins -
Synaptic Systems monoclonal
IgG1
Mouse
(purified IgG or ascites, lyophilized.Also available fluorescence-labeled, 110011C3)
ELISA, IC, IH, IP, WB Mammal (various), Chicken, Zebra fish, Human, Mouse, Rat immunogen = recombinant protein containing the N-terminal part of rat syntaxin 1 specific for Syntaxin 1A and B with preference for syntaxin 1B -
Synaptic Systems polyclonal
Rabbit
(antiserum, lyophilized.)
IC, IH, IP, WB Human, Mouse, Rat, Electric ray immunogen = synthetic peptide KYLPGPLQDMFKK (aa 122 - 134 in human) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue recognizes complexin 1 and 2 -
BD Transduction monoclonal
IgG1
Mouse
(aqueous buffered solution, BSA, glycerol, azide)
IF, IH, IP, WB Human, Mouse, Rat immunogen = Rat Munc-18-1, aa. 381-567, near the C-terminal recognizes Munc-18, 68 kDa -
BD Transduction monoclonal
IgG2b
Mouse
(aqueous buffered solution, BSA, glycerol, azide)
IF, IH, WB Mouse, Rat - recognizes rabphilin-3A, ~75 kDa -
Synaptic Systems monoclonal
IgG1
Mouse
(purified IgG, lyophilized)
IC, WB Various vertebrate, Human, Mouse, Rat immunogen = recombinant full length rat Rab 3a (epitope localized to a stretch of amino acids in the middle of the molecule, aa 95 - 151) specific for all four Rab 3 isoforms a, b, c d -
Synucleins From labs (3)
α-Synuclein LB509(Takeshi Iwatsubo, Department of Neuropathology & Neuroscience, Grad. School of Pharmaceutical Sciences, University of Tokyo)Also see Abcam ab27766, SantaCruz sc-58480, and others.References:Tokuda, 2010Tsika, 2010Xu, 2002 monoclonal
Mouse
IH, WB Human (epitope at the C terminus of the protein, residues 115-122) detects α-Synuclein, C-terminal -
α-Synuclein SNL-1(Virginia Lee, University of Pennsylvania, CNDR, Philadelphia, PA)used in paper by Giasson, 1999 polyclonal
Rabbit
- - immunogen = synthetic peptides corresponding to amino acids 104-119 in α-syn   -
α-Synuclein SNL-4(Virginia Lee, University of Pennsylvania, CNDR, Philadelphia, PA)used in paper by Giasson, 1999 polyclonal
Rabbit
- - immunogen = a synthetic peptide corresponding to residues 2-12 of α-synuclein   -
Synucleins From manufacturers (4)
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
IF, IP, WB Human immunogen = aa 121-125 of human α-synuclein α-synuclein, 19kDa -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = recombinant protein corresp. to aa 1-140 representing full-length human α-synuclein recognizes α/ β/ γ-synuclein -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
IF, IH, IP, WB Human immunogen = Lewy bodies of human origin recognizes α-synuclein, 19kDa -
BD Transduction monoclonal
IgG1
Mouse
(liquid, BSA, glycerol, azide, positive control)
IF, WB Human, Mouse, Rat immunogen = aa. 15-123 of rat synuclein-1 Synuclein-1,mol. wt. = 19kDa -
Tau From labs (2)
Peter Davies, Department of Pathology, Albert Einstein College of MedicineReferences:Bhaskar K, 2010Vingtdeux, 2010Polydoro, 2009Adams, 2009Julien, 2008Planel, 2008Luo, 2007Roberson, 2007Herskovits, 2006 monoclonal
IgG2b
Mouse
WB Human epitope around phospho-Serine202 detects tau phosphor. at Serine 202 -
Tau-003(Peter Davies, Department of Pathology, Albert Einstein College of Medicine)References:Bhaskar K, 2010Planel, 2008Götz 2001Lewis, 2001 monoclonal
IgM
Mouse
(IC, IF, IH, IP, WB)
- Human (epitope around phosphor. Thr231); also sensitive to the conformation of the phosphor. tau tau phosphor. at Thr231; not reactive to normal tau; TG3 epitope (phosphorylated Thr231) is expressed in mitotic cells but not in quiescent cells, suggesting that mitotic phosphoepitopes may lead to conformational changes -
Tau From manufacturers (6)
Pierce Thermo Scientific monoclonal
IgG1κ
Mouse
(purified, no carrier or preservative)
ELISA, IH, WB Human immunogen = aa. around phosphorylated Thr231 detects Tau doubly phosphorylated at Thr231 and Ser235; weak recognition of phosphorylation at Thr231 only -
Biosource Invitrogen monoclonal
IgG1
Mouse
(ascites, affinity purified BSA, azide, unconj.)
ELISA, IH, IP, WB Cow, Sheep, Human, Mouse, Rat immunogen = purified bovine microtuble associated proteins; epitope in middle region of tau tau proteins ranging from 45-68 kDa, not other MAPs or tubulin; tangles marker; reacts with non-phosphor. and phosphor. tau -
Autogen Bioclear kit
Mouse
(solid-phase enzymeimmunoassay, uses monoclonal AT120 for capture, HT7 and BT2 for incubation, streptavidin)
ELISA Human - tau in cerebrospinal fluid -
Innogenetics monoclonal
Mouse
ELISA, IH, WB Bovine, Human immunogen = purified human tau; (epitope within aa 159-163) phosphorylated and non phosphorylated tau -
Innogenetics monoclonal
IgG1κ
Mouse
(liquid, sterile filtered, PBS, no preservative, affinity purified)
ELISA, IH, WB Human immunogen = human tau40, aa. around phospho. Thr 181 specific for tau phospho at Thr 181; as PHF-tau, tangles and neurofilaments; weak cross with normal tau; -
Innogenetics kit
Mouse
(solid-phase enzymeimmunoassay, uses monoclonal AT120 for capture, HT7 and BT2 for incubation. Streptavidin detection of the antibodies.)
ELISA Human - tau in cerebrospinal fluid -
Tools From manufacturers (2)
Roche Applied Science monoclonal
IgG1, κ
Mouse
(mixture of two monoclonal antibodies, supplied as a white lyophilizate)
IP, WB - - can be used to verify the expression of Green Fluorescent Protein (GFP) and GFP fusion proteins; recognizes both wild-type and mutant forms of GFP -
Santa Cruz monoclonal
IgG1
Mouse
(gelatin, PBS, azideAvailable as phycoerythrin, agarose, HRP, fluorescein, rhodamine or biotin conj, and Alexa Fluor® 405, 488 or 647)
IF, IH, IP, WB Human, Mouse, Rat immunogen = peptide corresp. to aa 408-439 within the C-terminal domain of c-Myc of human origin detects c-Myc p67 -
Tubulin From manufacturers (1)
Sigma Aldrich monoclonal
IgG1
Mouse
(liquid, purified IgG, PBS, BSA, azideAlso available as ascites and as FITC conj.)
IC, IH, IP, RIA, WB Bovine, Chicken, Human, Mouse immunogen = purified chick brain tubulin (epitope at aa 426-430) recognizes an epitope located at the C-terminal end of alpha-tubulin -
p38 MAPK & MAPK11-14 From manufacturers (3)
Cell Signaling monoclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol)
FC, IC, IP, WB Mink, Human, Monkey, Mouse, Rat Immunogen = synthetic phospho-peptide corresp. to residues surrounding Thr180/Tyr182 of human p38 MAP kinase conj. to KLH Detects endogenous levels of p38 MAP kinase only when dually phosphorylated at Thr180 and Tyr182. Does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK -
Cell Signaling polyclonal
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, Protein A and peptide affinity purified)
FC, IF, IP, WB Pig, Human, Monkey, Mouse, Rat, S. cerevisiae, Drosophila Immunogen = synthetic phospho-peptide corresp. to residues around Thr180/Tyr182 of human p38 MAP kinase Detects endogenous levels of p38 MAP kinase only dually phosphorylated at Thr180 and Tyr 182. Does not cross react with phosphorylated forms of p42/44 MAPK or SAPK/JNK -
Cell Signaling polyclonal
Rabbit
(liquid,, HEPES, NaCl, BSA, glycerol, protein A and peptide affinity purified)
FC, IF, IH, WB Human, Monkey, Guinea Pig, Mouse, Rat Immunogen = synthetic peptide derived from the seq. of human MAP kinase, coupled to KLH Detects endogenous levels of total p38 MAP kinase protein. Does not cross-react with p38δ, JNK/SAPK or p44/42 MAP kinase -