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Antibodies

29333 Results

Name Source Clonality &
Isotype
Host &
Formulation
Method Reactivity Immunogen/
Epitope
Specificity Availability
ABC proteins/ transporters From manufacturers (1)
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
IH, IP, WB Human, Monkey, Mouse immunogen = synthetic peptide: generated from within residues 1200-1300 of human ABC 1 ABC1 -
ADAMS & ADAMTS From manufacturers (9)
Calbiochem EMD Biosciences polyclonal
IgG
Rabbit
(liquid, undiluted serum, no preservative)
WB Human, Hamster, Mouse immunogen = synthetic peptide C-RQRPRESYQMGHMRR corresponding to amino acids 735-749 of mouse ADAM10 recognizes the ~98 and ~105 kDa bands corresponding to full length ADAM1O in transfected CHO-K cells, the ~60 kDa cleaved mature protein and the ~30 kDa degradation product -
EMD Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, PBS, azide)
WB Human, Mouse, Rat immunogen = synthetic peptide (ASFKLQRQN RVDSKETE) corresponding to amino acids 807-823 of human TACE recognizes the ~130 kDa TACE protein in HeLa and Jurkat cells -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, protein stabilizer)
ELISA, IP, WB Human immunogen = full length recombinant TACE of human origin for detection of TACE; no cross with ADAM8, ADAM9 or ADAM15 -
Santa Cruz polyclonal
IgG
Goat
(liquid, affinity purified, PBS, azide, gelatin)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = apeptide mapping at the C-terminus of TACE of human origin for detection of precursor and mature TACE -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = amino acids 235-404 mapping within an extracellular domain of TACE of human origin for detection of TACE -
Santa Cruz polyclonal
IgG
Goat
(liquid, affinity purified, PBS, azide, gelatin)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = apeptide mapping near the C-terminus of TACE of human origin for detection of precursor and mature TACE -
Cell Signaling monoclonal
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
WB Human, Monkey, Mouse, Rat Immungoen = a synthetic peptide corresponding to residues near the carboxy terminus of human ADAM9 detects endogenous levels of total ADAM9 protein -
Chemicon and Upstate are Millipore polyclonal
Rabbit
(liquid, PBS, azide, purified)
IC, WB Human, Mouse Immungoen = a peptide corresponding to amino acids 807-823 of human TACE (differs from mouse, rat by 1 aa.) detects ADAM17 /TACE -
Cell Signaling polyclonal
Rabbit
(liquid, affinity purified, HEPES, saline, BSA, glycerol)
IP, WB Human, Monkey, Mouse, Rat Immungoen = a synthetic peptide corresponding to the carboxy terminus of human ADAM9 detects endogenous levels of total ADAM9 protein; , unprocessed and active forms. The antibody does not recognize the carboxy terminally truncated short form of ADAM9 -
APP, APLP, AICD From labs (6)
(Konrad Beyreuther, Center of Molecular Biology, University of Heidelberg, Heidelberg, Germany)PaperReference: Kuhn, 2010 monoclonal
Mouse
IH, WB Human, Monkey, Mouse, Rat immunogen = a bacterial APP fusion protein (Fd-APP 695), (epitope in ectodomain, at residues 66-81 of APP) detects a variety of mature as well as immature APP species which differ in their degree of glycosylation -
(Stefan Lichtenthaler, Adolf Butenandt Institute, Ludwig Maximilians University, München, Germany)PaperReference: Kuhn, 2010 monoclonal
IgG2a
Unknown Host
WB Human, Mouse immunogen = a peptide comprising amino acids 10–15 of the Aβsequence (YEVHHQ-COOH) specifically detects APPsα; detects also murine APPsα, but notAPPsβ and APPsβ' -
(Stefan Lichtenthaler, Adolf Butenandt Institute, Ludwig Maximilians University, München, Germany)PaperReference: Kuhn, 2010 monoclonal
IgG2a
Rat
WB Human immunogen = a peptide comprising amino acids 11–16 of the Aβsequence (EVHHQK-COOH) specifically detects APPsα ending in amino acids 15 and 16 (APPs-15 and APPs-16), but does not detect shorter APPs species, including APPsβ' and APPsβ; does not detect full-length APP -
6687Christian Haass, Ludwig-Maximilians-Universität München, Munich, GermanyPaper, Steiner et al.Reference: Kojro, 2006 polyclonal
Unknown Host
- Human immunogen = the 20 most C-terminal amino acids of human APP human APP C-terminal -
APP B5 (5313)(Christian Haass, Ludwig-Maximilians-Universität München, Munich, GermanyPaper, Steiner et al.References:Kuhn, 2010Stamer, 2002 polyclonal
Unknown Host
IP Human immunogen = a sequence in the ectodomain, aa 444-592, of human APP reactive with APPs, ectodomain -
CT15(S Soriano, 2001Paper, JBC online,Dr. Edward Koo, University of California, San Diego),References:Lee, S, 2010Banwait, 2008Rockenstein, 2007Galvan, 2006 polyclonal
Rabbit
IP, WB Human, Mouse immunogen = syntheticpeptide corresp. to the C-terminal 15 residues of APP (aa 680-695) APP C-terminal fragments; recognizes the last 15 COOH-terminal amino acid residues of APP695 -
APP, APLP, AICD From manufacturers (2)
Sigma Aldrich polyclonal
IgG
Rabbit
(IgG frac, PBS, azide)
IB, IH Human, Mouse, Rat immunogen = synthetic, aa 46-60 of the N terminus of APP695 APP695, APP751 and APP770 -
Epitomics monoclonal
Mouse
(liquid, Tris-Glycine, NaCl, Glycerol, BSA, azide)
FC, IC, IH, IP, WB Human, Mouse, Rat immunogen = synthetic peptide corresponding to residues in the NPXY motif of human APP specifically recognizes APP and its isoforms; will not recognize either of the two Amyloid Beta Protein (Aβ4) variants, Aβ1 -40 or Aβ1 -42 -
Actin Related From manufacturers (2)
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purifiedAlso available as:sc-1616-R (rabbit)sc-1616 FITC sc-1616 PEsc-1616 TRICT sc-1616 B (biotin)sc-1616 HRPsc-1616 AC Cat# sc1616 AF405, AF488, AF647; Alexa Fluor®)
FC, IF, IH, IP, WB Mammal (various), Dog, Zebra fish, Cow, Horse, Pig, Human, Mouse, Rat, Xenopus, C. elegans, S. cerevisiae, Drosophila immunogen = peptide mapping at the C-terminus of human actin broad range of Actin isoforms -
Invitrogen Corporation monoclonal
IgG2a
Mouse
(liquid ascites, PBS, BSA, azide)
IH Human, Mouse immunogen = Actin synthetic peptide α isoform of smooth muscle actin; no cross reaction with skeletal and cardiac actin, mesenchymal cells, epithelial cells or other cytoskeletal components -
Amyloid-β From labs (9)
anti-amyloid beta FCA3340(Frédéric Checler,Centre National de la Recherche Scientifique),PaperReference: Gandy, 2010 polyclonal
Rabbit
DB, ELISA, IP, WB Human, Mouse immunogen = synthetic octapeptide Cys-GLMVGGVV, mimicing the C-terminal end of Aβ 40, coupled to either BSA or KLH amyloid-beta 40; labels leptomeningeal and cortical arterioles;little or no cross with Aβ 42 -
FCA3542(Frédéric Checler,Centre National de la Recherche Scientifique),PaperReferences:Gandy, 2010Lesné, 2006 polyclonal
Rabbit
(serum)
DB, ELISA, IP, WB Human, Mouse immunogen = synthetic octapeptide Cys-MVGGVVIA, mimicing the C-terminal end of Aβ 42, coupled to either BSA or KLH recognizes amyloid-beta 42; little or no cross with Aβ 40; intensely labels both diffuse and mature lesions -
JRF/rAβ/2 (Paul Mathews, New York University, School of Medicine,Center for Dementia Research)Research paperReference: Boland, 2010 monoclonal
Mouse
(liquid, horseradish peroxidase conj.)
ELISA, WB Mouse immunogen = a peptide corresponding to murine Aβ residues 1–15 (DAEFGHDSGFEVRHQ) recognizes mouse but not human Aβ1–40 or Aβ1–42 -
JRF/Aβtot/17(Paul Mathews, New York University, School of Medicine,Marc Merken,Johnson & Johnson Pharm. R&D,paper)References:Gandy, 2010Trinchese, 2008Mi, 2007 monoclonal
Mouse
ELISA, IB Human immunogen = human Aβ1-40 amyloid-beta, binds within residues 1-15 -
JRF/rAβ1-15/2(Paul Mathews, New York University, School of Medicine,Marc Merken,Johnson & Johnson Pharm. R&D,paper)References:Gandy, 2010 monoclonal
Mouse
ELISA Mouse epitope within residues 1-15 of mouse Aβ binds to amyloid-beta -
JRF/cAβ40/10(Paul Mathews, New York University, School of Medicine,Marc Mercken,Johnson & Johnson Pharm. R&D,Paper)References:Boland, 2010Trinchese, 2008Mi, 2007Razmahel, 2002 monoclonal
Mouse
ELISA, WB Human, Mouse immunogen = a peptide corresponding to Aβ residues 36–40 (VGGVV) recognizes the carboxyl terminus of Aβ40; does not detect Aβ1–42 by ELISA or WB, or full-length APP by IP or WB -
JRF/cAβ42/26(Marc Mercken,Johnson & Johnson Pharm. R&D,Paper)References:Boland, 2010Trinchese, 2008Mi, 2007Razmahel, 2002 monoclonal
Mouse
ELISA Human, Mouse immunogen = the carboxy terminal 10 residues of Ab1-42 amyloid-beta 42, C-terminus -
(Konrad Beyreuther, Center of Molecular Biology, University of Heidelberg, Heidelberg, Germany)PaperReference: Kuhn, 2010 monoclonal
IgG2a
Mouse
ELISA, IH, WB Human, Guinea Pig immunogen = decapeptides overlapping each other by one aminoacid, covering residues 1-42 of Aβ (epitope mapping corr. to amino acid residues 4-10 of human Aβ-peptide) High affinity to Amyloid peptides Aβ 1-38, Aβ 1-39, Aβ 1-40 -
3552(Harald Steiner,Laboratory for Alzheimer’s and Parkinson’s Disease Research, Adolf ButenandtInstitute, Ludwig Maximilians University, Munich, Germany)PaperReference: Kuhn, 2010 polyclonal
Unknown Host
IP, WB Human immunogen = Aβ1–40 recognizes amyloid-beta -
Amyloid-β From manufacturers (5)
Biosource Invitrogen monoclonal
IgG
Rabbit
(liquid, PBS, azide)
ELISA, IH Human, Mouse immunogen = peptide (VGGVVIA) corresp. to aa707-713 of P05067 specific to Amyloid β1-42; not Amyloid β1-40, Amyloid β1-43, Amyloid β1-37 or Amyloid β1-38 -
Biosource Invitrogen monoclonal
IgG
Rabbit
(liquid, PBS, azide, protein A purified)
ELISA, IH Human immunogen = aa residues from the C-terminus of human amyloid beta A4 protein specific to Amyloid β42; no cross reaction with Amyloid beta 1-40 -
- monoclonal

(supernat, RIA screened, HPLC purified)
IH, WB - immunogen = synthetic peptide fragment derived from different regions of amyloid-beta and coupled to a carrier protein amyloid-beta -
Genetics Company, Inc. monoclonal
IgG2b
Mouse
(liquid, supernatant, stabilizing solution, affinity purified, conj. HRP)
ELISA, WB Dog, Human, Guinea Pig, Mouse, Rat, Cynomolgus C-terminal epitope mapping corr. to amino acid residues 31-40 of human Aβ peptide recognizes Aβ-peptide, aa 31-40; selective for C-terminal of Aβ 1-40; not Aβ 1-38, Aβ 1-39, Aβ 1-42, Aβ 1-43 or Aβ 1-44 -
Genetics Company, Inc. monoclonal
IgG1
Mouse
(liquid, stabilizing solution, no azide, affinity purified, conj. HRP)
ELISA, IH, WB Dog, Human, Guinea Pig, Mouse, Rat, Cynomolgus immunogen = Aβ 1-42 peptide at the C-terminus C-terminal of Aβ 1-42 peptide; not Aβ 1-38, Aβ 1-39, Aβ 1-40; low cross reactivity to Aβ 43 (10%)and Aβ 44 (13%) -
Apolipoproteins From manufacturers (3)
Chemicon and Upstate are Millipore polyclonal
IgG1
Goat
(serum, filtered, tris-saline, azide)
IH, WB Primate (various), Human, Mouse immunogen = recombinant human apolipoprotein E Apo E isoforms E2, E3 and E4 -
Santa Cruz polyclonal
IgG
Goat
(liquid, affinity purified, PBS, azide, blocking peptide)
IH, IP, WB Mouse, Rat immunogen = a peptide mapping at the carboxy terminus of mouse ApoE Apo E -
Rockland polyclonal
Goat
(liquid, sterile filtered, borate, NaCl, EDTA, azide, affinity purified)
ELISA, IH, IP, WB Mouse immunogen = ApoA-I isolated from mouse plasma, HPLC purification reacts with mouse apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins -
Astrocyte Markers From manufacturers (2)
Invitrogen Corporation monoclonal
IgG1
Mouse
(liquid, tissue culture supernatant, PBS, BSA, azide, concentrate.Also available predilute, ready to use, and 2ndGen predilute, ready to use)
IH Human, Mouse immunogen = human brain reacts with GFAP-containing cells, and labels only the 56 kDa GFAP band from immunoblots; no cross with neurofilament, cytokeratin, vimentin, or desmin; useful in the identification of benign astrocytes and neoplastic cells of glial lineage -
Sigma Aldrich polyclonal
IgG
Rabbit
(liquid, buffered aqueous solution, IgG fract., PBS, azide)
IH, WB Human, Rat immunogen = GFAP from human brain GFAP in astrocytes, glia cells (Bergmann glia), gliomasand other glial cell derived tumors -
CD45 From manufacturers (1)
AbD Serotec monoclonal
IgG2b
Rat
(liquid, PBS, azide, BSA, supernate, protein G purifiedSee list of conjugations)
FC, IH, IP Mouse immunogen = mouse spleen cells reacts with CD45 (180-220kDa) -
COX1, COX2, COX3, COX IV From manufacturers (3)
Accurate polyclonal
Goat
- Mammal (various), Rodent (various), Sheep   COX-1 -
Molecular Probes Invitrogen monoclonal
IgG2a
Mouse
(serum-free medium, purified bybiochemical fractionation, HBS, azide)
IC, WB Human immunogen = Human Complex IV subunit II, from SDS-PAGE recognizes COX-2 -
Invitrogen Corporation monoclonal
IgG2a
Mouse
(serum-free medium, purified bybiochemical fractionation, HBS, azide)
IC, WB Bovine, Zebra fish, Human, Mouse, Rat, C. elegans immunogen = Human Complex IV subunit I (COX1) recognizes COX-1 -
Cell Cycle From manufacturers (3)
Biosource Invitrogen polyclonal
IgG
Rabbit
(liquid Ig, Dulbecco’s PBS, glycerol, BSA, azide, affinity purifiedAlso available Alexa Fluor® 488 for IF, Cat# 44-660A1)
IH, WB Chicken, Human, Mouse immunogen = a chemically synthesized phosphopeptide derived from the region of human Src that contains tyrosine 418 (tyrosine 419 including the initiating methionine) detects Src; also reacts with Fyn and Yes -
Biosource Invitrogen polyclonal
IgG
Rabbit
(liquid Ig, Dulbecco’s PBS, glycerol, BSA, azide, affinity purified)
IH, WB Chicken, Human, Mouse, Rat immunogen = a chemically synthesized phosphopeptide derived from the region of human Src that contains tyrosine 529 (tyrosine 530 including the initiating methionine). detects Src; not tested for reaction with Fyn and Yes -
Santa Cruz monoclonal
IgG1
Mouse
(liquid IgG, PBS, azide, gelatinAlso available as sc-434PE for FC, and sc-434AC for IP)
FC, IF, IH, IP, WB Human, Mouse, Rat epitope mapping between amino acids 85-206 of Fyn p59 of human origin detects Fyn p59 -
Cholesterol Related From manufacturers (1)
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide.Also available conjugated with biotin, NB400-148B;HRP, NB400-148H;DyLight 649, NB400-148C;DyLight 549, NB400-148R;DyLight 488, NB400-148G)
IF, IH, IP, WB Human, Hamster, Mouse immunogen = synthetic peptide: made to the C-terminal region of human NPC1. specific for NPC1 protein -
GABA & Related From manufacturers (1)
Chemicon and Upstate are Millipore monoclonal
IgG2a
Mouse
(phosphate buffer, protein-A purified Ig, azide)
IH, WB Human, Mouse, Rat immunogen = recombinant fusion protein containing the unique N terminal regions of GAD67 not shared by GAD65 protein Reacts with the 67kDa isoform of Glutamate Decarboxylase (GAD67) -
Glutamate Receptor & Related From manufacturers (2)
Calbiochem EMD Biosciences polyclonal
IgG
Rabbit
(liquid, phosphate buffer, gelatin, azide)
IF, WB Rat Immunogen = synthetic peptide (RTSDSRDH TRVDWKR) corresp. to aa271-285 of rat GluR1 (numbered from the signal peptide) recognizes the ~105-107 kDa GluR1 protein. Also cross-reacts with a 50 kDa protein in rat brain extracts -
BD Pharmingen monoclonal
IgG2a
Mouse
(liquid, buffer, azide)
EM, IH, RIA, WB Dog, Monkey, Rat Immunogen = recombinant trpE fusion protein containing the putative N-terminal aa 175-430 of GluR2 GluR2 -
Glycolysis/Respiration From manufacturers (1)
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, purified Ig, PBS, azide)
ELISA, IC, WB Fish (various), Cat, Rabbit, Chicken, Frog, Human, Mouse, Rat, S. cerevisiae immunogen = GAPDH from rabbit muscle 38 kDa GAPDH from skeletal muscle; also cardiac GAPDH; GAPDH enzyme is detected in many non-muscle cells lines including HeLa, HCT-116 cells, U937 and THP-1 cells among others -
Huntington's-related From labs (1)
(Sungmin Song, Department of Life Science, Kwangju Institute of Science and Technology, Kwangju, Korea)PaperReference: Ko, S, 2010 polyclonal
Rabbit
(liquid, affinity purified)
IC, IH, NB, WB Human, Mouse immunogen = synthetic peptide (MANIAVQRIKREC)spanning amino-terminal 13 amino acids of E2-25K/Hip-2, conjugated with carrier protein, KLH reacts with E2-25K/Hip-2 -
Ion Channels From manufacturers (1)
Abcam monoclonal
IgG2a
Mouse
(liquid, PBS, no preservative, protein A purified)
ELISA, WB Human immunogen = recombinant fragment corresp. to aa1-100 of human VDAC1 / Porin VDAC/porin -
Kinases From manufacturers (4)
Cell Signaling monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol)
IH, WB Human, Monkey, Mouse, Rat immunogen = synthetic peptide corresponding to residues surrounding the amino-terminus of human p70 S6 kinase detects endogenous levels of total p70 S6 kinase protein. This antibody also recognizes p85 S6 kinase -
Cell Signaling monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol.Also available Biotinylated, Cat# 5707)
IH, WB Human, Monkey, Mouse, Rat immunogen = synthetic peptide corresponding to residues surrounding the amino-terminus of human p70 S6 kinase detects endogenous levels of total p70 S6 kinase protein. This antibody also recognizes p85 S6 kinase -
Cell Signaling polyclonal
Rabbit
(liquid, protein-A and affinity purified, HEPES, saline, BSA, glycerol)
IP, WB Human, Monkey, Mouse, Rat immunogen = synthetic peptide (KLH-coupled) derived from residues surrounding the carboxy terminus of human p70 S6 kinase detects endogenous levels of total p70 S6 kinase protein. This antibody also recognizes p85 S6 kinase -
Cell Signaling monoclonal
IgG2a
Mouse
(liquid, protein-A and affinity purified, HEPES, saline, BSA, glycerol, azide)
WB Human, Monkey, Mouse, Rat, Drosophila immunogen = synthetic phospho-peptide (KLH-coupled) corresponding to residues around Thr389 of human p70 S6 kinase detects endogenous levels of p70 S6 kinase only when phosphorylated at Thr389; also detects p85 S6 kinase when phosphor. at the analogous site (Thr412) and possibly S6KII phosphor. at Thr401 -
MAPs From manufacturers (5)
Sigma Aldrich monoclonal
IgG1
Mouse
(ascites fluid, azide)
IB, IH Bovine, Chicken, Quail, Human, Mouse, Rat immunogen = rat brain MAPs all forms of MAP2; not tubulin, or any other maps; selective labeling of dendritic trees throughout the brain -
nanoTools Antikörpertechnik GmbH & Co. monoclonal
IgG1
Mouse
(liquid)
IC, WB Human, Monkey, Hamster, Mouse, Rat immunogen = synthetic peptide conjugated to hemocyanin (epitope at N-Terminus of LC3-B) recognizes MAP1 light chain component LC-3 -
Cell Signaling polyclonal
Rabbit
(liquid, protein A and affinity purified)
FC, IC, IF, WB Human, Mouse, Rat Immunogen = synthetic peptide corresp. to residues surrounding Gly40 of LC3A/B detects endogenous levels of total LC3A and LC3B proteins. Cross-reactivity may exist with LC3C. -
Cell Signaling monoclonal
IgG
Rabbit
(liquid, HEPES, saline, glycerol, azide)
FC, IC, IF, IH, WB Human, Mouse, Rat Immunogen = synthetic peptide corresp. to residues near the amino terminus of LC3B detects endogenous levels of total LC3B protein. Cross-reactivity occur with other LC3 isoforms -
Cell Signaling monoclonal
IgG
Rabbit
(liquid, HEPES, saline, glycerol, azide)
FC, IC, IF, IH, IP, WB Human, Mouse, Rat Immunogen = synthetic peptide corresp. to residues near the amino terminus of LC3A detects endogenous levels of total LC3A protein Cross-reactivity occur with LC3B -
Miscellaneous From manufacturers (2)
Chemicon and Upstate are Millipore polyclonal
IgG
Rabbit
(liquid, affinity purified, buffer, PBS, azide)
ELISA, IH, WB Bovine, Pig, Human, Mouse, Rat immunogen = synthetic peptide corresponding to the C-terminus (amino acids 498-510) of mouse GLUT-4 recognizes GLUT4; ~55 kDa by WB -
A D I polyclonal
IgG
Rabbit
(affinity purified, buffer, PBS, BSA, lyophilizedAlso available as unpurified antiserum, GT11-S)
ELISA, IH, WB Mouse immunogen = 12-aa peptide mouse GT11, conjugated to KLH; epitope location~ C-terminal, Cytoplasmic domain recognizes GLUT1 -
Organelles From manufacturers (1)
Abcam monoclonal
IgG1
Mouse
(liquid, IgG fraction, borate, no preservativeAlso available as Phycoerythrine conj. ab25367)
FC, IF, IH, WB Human, Monkey, Mouse - recognizes LAMP1, ~48 kDa by WB -
PSD95 From manufacturers (1)
NeuroMab monoclonal
IgG2a
Mouse
(antiserum, affinity purified, PBS, azide)
IB, IF, IH, IP Human, Mouse, Rat immunogen = fusion protein amino acids 77-299 of human PSD-95/SAP90 (accessionnumber P78352) recognizes the ~95-110 kDa PSD-95 protein; no cross against recombinant Chapsyn-110, SAP97 or SAP102 -
Post-synaptic proteins From manufacturers (2)
Sigma Aldrich polyclonal
IgG
Rabbit
(IgG fraction of antiserum, buffered aqueous solution, PBS, azide)
WB Human, Rat immunogen = synthetic peptide corresponding to the N-terminal region of human drebrin E/A (amino acids 22-42) conjugated to KLH. reacts with Drebrin -
Abcam polyclonal
IgG
Mouse
(liquid, protein-A purified, PBS, no preservativeAlso available anti-N-term Homer, ab85269)
WB Human, Rat immunogen = full length human Homer protein (NP_004263, amino acids 1-354) detects Homer, approximately 39 kDa in WB -
Posttranslational Modifications From labs (2)
Ubi-B1 and Ubi-B2(Fred W. van Leeuwen, Netherlands Institute forBrain Research, Amsterdam, Netherlands)Paper polyclonal
Rabbit
(See also R&D systems antibodies MAB703 andAF703)
IB, IC, IH Human immunogen = synthetic peptides covering YADLRED PDRQDHH PGSGAQ at the C-terminus of Ubiquitin+1 reacts with Ubiquitin+1 and not Ubiquitin -
(Sungmin Song, Department of Life Science, Kwangju Institute of Science and Technology, Kwangju, Korea)PaperReference: Ko, S, 2010 monoclonal
Rat
(liquid, affinity purified)
IC, IH, WB Human immunogen = synthetic peptide of the same amino acid sequence as previously described in van Leeuwen et al., 1998 reacts with Ubiquitin+1 and not Ubiquitin -
Posttranslational Modifications From manufacturers (1)
R&D Systems monoclonal
IgG2A
Mouse
(IgG fract, protein-G, PBS, filtered, lyophilized)
WB Human immunogen = KLH coupled synthetic peptide CADLRED PDRQDHH PGSGAQ corr. to the C-terminus of Ubiquitin+1 Ubiquitin+1; not Ubiquitin -
Protein Trafficking From manufacturers (5)
Cell Signaling polyclonal
Rabbit
(liquid, affinity purified)
IC, IF, IP, WB Human, Monkey, Mouse, Rat Immunogen = synthetic peptide corresp. to residues surrounding Gly14 of human EEA1 protein detects endogenous levels of total EEA1 protein -
Cell Signaling monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
IF, IP, WB Human, Mouse, Rat Immunogen = synthetic peptide corresp. to residues surrounding Ser70 of human EEA1 protein detects endogenous levels of total EEA1 protein -
Abcam polyclonal
IgG
Rabbit
(liquid, whole antiserum, no preservative)
IH Mouse, Rat immunogen = a synthetic peptide from the internal part of mouse Mannose 6 Phosphate Receptor (Cation independent) conjugated to an immunogenic carrier protein detects Cation independent Mannose 6 Phosphate Receptor -
Abcam polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, saline, glycerol, azide)
ELISA, IF, IH Human, Mouse immunogen = synthetic non-phosphopeptide derived from human Mannose 6 Phosphate Receptor (Cation independent) around the phosphorylation site of serine 2484 (D-D-S-D-E) detects endogenous levels of total Cation independent Mannose 6 Phosphate Receptor -
Abcam polyclonal
IgG
Rabbit
(liquid, whole antiserum, azide)
IC, IF, IH, IP, WB Cow, Pig, Human, Mouse immunogen = full length native protein purified from adult Bovine liver tissue detects Cation independent Mannose 6 Phosphate Receptor, ~300 kDa by WB -
SOD From manufacturers (4)
MBL International monoclonal
IgG1
Mouse
(protein-A purified, no preservative, lyophilized)
WB Human Immunogen = GST-full length humanSOD1 fusion protein CuZn/SOD -
MBL International polyclonal
IgG
Rabbit
(purified, PBS, BSA, glycerol, azide)
IH, IP, WB Human, Mouse, Rat - CuZn/SOD -
Assay Designs is Enzo Life Sciences polyclonal
Rabbit
(liquid, protein-A affinity purified, PBS, glycerol, azide)
WB Human, Mouse, Rat Immunogen = native rat Cu/Zn SOD-1 recognizes Cu/Zu SOD -
Assay Designs is Enzo Life Sciences polyclonal
Rabbit
(liquid, protein-A affinity purified, PBS, glycerol, azide)
IF, IH, IP, WB Human, Mouse, Rat, Clam Immunogen = native human Cu/Zn SOD-1 recognizes Cu/Zu SOD -
Secreted Neuropeptides & Receptors From manufacturers (2)
Immunostar polyclonal
IgG
Rabbit
(whole serum, azide, lyophilized)
IF, IH Avian (various), Fish (various), Cat, Frog, Human immunogen = neuropeptide Y; coupled to bovine thyroglobulin (BTg) with glutaraldehyde specific to NPY; stains paraventricular nucleus, cortex and caudate putamen -
Chemicon and Upstate are Millipore monoclonal
IgG2b
Rat
(liquid, culture supernatant, thimerosal)
IF, IH Rabbit, Human, Rat immunogen = synthetic 1-14 cyclic somatostatin conjugated to bovine thyroglobulin using carbodiimide reacts with somatostatin. Shows no cross reactivity to enkephalins, other endorphins, substance P or CGRP. Partially cross-reacts with somatostatin fragments -
Synaptic Vesicle Fusion From manufacturers (1)
Assay Designs is Enzo Life Sciences monoclonal
IgG2a
Mouse
(liquid, PBS, glycerol, azide, protein G purified)
IC, IF, IH, IP, WB Mouse, Rat immunogen = recombinant rat bassoon recognizes bassoon -
Tau From manufacturers (3)
Biosource Invitrogen kit
Unknown Host
(contains:Hu Tau [pT181]Antibody Bead Concentrate;Hu Tau [pT181]Detection Ab Concentrate;Hu Tau [pT181] Standard)
ELISA Human human tau-derived peptide that is phosphorylated at threonine 181 for the measurement of human Tau when phosphorylated at threonine 181 in CSF and tissueculture media -
Pierce Thermo Scientific monoclonal
IgG1κ
Mouse
(frozen liquid, no carrier or preservative, biotin conj.)
ELISA, IH Bovine, Human immunogen = human tau, epitope between aa 159 and 163 tau and PHF tau tau between aa 159-163 -
Chemicon and Upstate are Millipore monoclonal
IgG2a
Mouse
(purified Ig, phosphate buffered, lyophilized)
IH, WB Bovine, Human, Rat immunogen = purified denaturated bovine MAPs (epitope at or near a phosphorylated site) binds to all known electrophoretic species of tau in human, rat and bovine brain (one-dimensional SDS-PAGE); there is some unphosphorylated bias with clone PC1C6 as it seem to recognize only dephosphorylated serine sites at 195, 198, 199, and 202 -
Tools From manufacturers (13)
Sigma Aldrich monoclonal
IgG1
Mouse
(purified Ig, buffered aqueous solution, PBS, BSA, azide, FITC)
IF Various species, Mouse, Rat - Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent; useful in detection of FLAG fusion proteins expressed in murine host -
Zymed/ Invitrogen monoclonal
IgG1
Mouse
(liquid, affinity purified, PBS, azide)
WB Non-specific immunogen = purified recombinant GFP protein from the jellyfish Aequorea victoria reacts with GFP -
Vector Labs polyclonal
Goat
(liquid, affinity purified, HEPES, sodium sulfate, thimerosal)
WB Non-specific - for direct detection of GFP fusion proteins on western blots usingchromogenic or chemiluminescent peroxidase substrates -
Sigma Aldrich monoclonal
IgG1
Mouse
(affinity isolated, buffered aqueous solution, phosphate, glycerol, no antimicrobial preservatives)
IB, IC, IH, IP Various species - Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent; optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems -
Sigma Aldrich monoclonal
IgG1
Mouse
(purified IgG, buffered aqueous solution, phosphate, saline, azide)
ELISA, IP, WB Various species - Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent -
MP Biomedicals monoclonal
IgG1
Mouse
(purified from the supernatant of mouse hybridomas; store at -80°C)
ELISA, WB Non-specific immunogen = green fluorescent protein (GFP) allows detection of as little as 10 ng of GFP using colorimetric methods, and will detect picogram quantities with chemiluminescentdetection assays -
Molecular Probes Invitrogen polyclonal
IgG
Rabbit
(liquid, IgG fraction, Alexa Fluor® 488 conjugateAlso available asAlexa Fluor® 555, A31851;Alexa Fluor® 594, A21312;Alexa Fluor® 647, A31852)
IF Non-specific - reacts with GFP -
Molecular Probes Invitrogen polyclonal
IgG
Rabbit
(liquid, antiserum, thimerosal, unonjugated)
FA, WB Non-specific immunogen = GFP isolated directly from the jellyfish Aequorea victoria reacts with GFP -
Molecular Probes Invitrogen monoclonal
IgG2a
Mouse
(purified from mouse hybridoma supernatants by protein G chromatography and stabilized with BSA, unonjugated, lyophilized)
ELISA, IH, IP Non-specific immunogen = green fluorescent protein (GFP) that was isolated directly from Aequorea victoria specific for GFP -
Molecular Probes Invitrogen monoclonal
IgG1
Mouse
(purified from mouse hybridoma supernatants by protein G chromatography and stabilized with BSA, unonjugated, lyophilized)
FA, WB Non-specific immunogen = green fluorescent protein (GFP) that was isolated directly from Aequorea victoria reacts with GFP -
Molecular Probes Invitrogen polyclonal
IgG
Rabbit
(liquid, IgG fraction, PBS, azide)
IF Non-specific immunogen = green fluorescent protein (GFP) that was isolated directly from A. victoria and purified by ion-exchange reacts with GFP -
MP Biomedicals monoclonal
IgG2a
Mouse
(purified from the supernatant of mouse hybridomas; store at -80°C)
ELISA, IC, IP Non-specific immunogen = green fluorescent protein (GFP) highly specific for GFPs in both bacterial and mammalian cell extracts; allows purification of GFP and GFP-fusion proteins with either protein A or protein G -
Sigma Aldrich polyclonal
IgG
Rabbit
(affinity purified, buffered aqueous solution, IgG, PBS, BSA, azide)
DB, IF, WB Non-specific immunogen = Synthetic FLAG sequence containing peptide DYKDDDDK-GC conjugated through a terminal cysteine thiol to maleimide-activated KLH recognizes the FLAG epitope located onFLAG fusion proteins; reacts with Nterminal,N-terminal-Met, and C-terminal FLAG fusion proteins -
Tubulin From manufacturers (1)
Lab Vision Thermo Scientific monoclonal
IgG1
Mouse
(liquid, ascites, protein G purified, PBS, BSA, azide; available without BSA and azide)
EM, IF, IH, IP, WB Chicken, Frog, Cow, Pig, Human, Gerbil, Guinea Pig, Mouse, Rat immunogen = native chick brain microtubules; epitope aa426-450 57 kDa of α-tubulin and not β-tubublin -