VAT
A D I
polyclonal
IgG
Chicken
(neat serum, azide, available affinity purified)
-
Mouse, Rat
immunogen = twenty aa. peptide mapping within the C-terminus of rat/mouse VAT (75% homology with human VAT)
VAT; antibody labels cholinergic cell bodies in the septum and nucleus basalis and cholinergic fiber
-
VAChT
Affiniti - Biomol
polyclonal
Goat
(neat serum)
-
Mouse, Rat, Guinea Pig
immunogen = peptide corr. to the C-terminus of rat VAChT
VAChT
-
VAChT
Autogen Bioclear
polyclonal
IgG
Goat
(affinity purified, PBS, azide, gelatin)
-
Human, Mouse, Rat
immnuogen = peptide mapping at the C-terminus of human VAChT; differs from mouse seq. by 2 amino acids
VAChT
-
VAChT
Autogen Bioclear
polyclonal
IgG
Goat
(affinity purified, PBS, azide, gelatin)
-
Human, Mouse, Rat
immnuogen = peptide mapping at the N-terminus of human VAChT; differs from mouse seq. by single amino acid
VAChT
-
VAT
Biogenesis AdB Serotec
polyclonal
Ig
Goat
(purified Ig, sepharose, BSA, PBS, azide)
-
Mouse, Rat, Guinea Pig
immunogen = synthetic 20 aa sequence from rat VAT protein, conj.with thyroglobulin
VAT; does not react with vesicular monoamine transporter (VMAT)
-
VAT
Calbiochem EMD Biosciences
polyclonal
Ig
Goat
(antiserum, lyophilized)
-
Rat
immunogen = synthetic peptide corr. to aa 511-530 from cloned rat VAChT
VAT; not vesicular monoamine transporters
-
VAT
Cambio
polyclonal
IgG
Goat
(diluted serum)
-
-
VAT
-
VAT
Cambio
polyclonal
IgG
Sheep
(Ig)
-
-
VAT
-
VAChT
Euro-Diagnostica AB
polyclonal
Rabbit
(lyophilized)
-
Mouse, Rat
immunogen = synthetic peptide from the C-terminal of rat conjugated to BSA
VAChT
-
VAChT
Euro-Diagnostica AB
polyclonal
Rabbit
(lyophilized)
-
Human
immunogen = synthetic peptide from the C-terminal of human conjugated to BSA
VAChT
-
VAChT
Euro-Diagnostica AB
polyclonal
Guinea Pig
(lyophilized)
-
Mouse, Rat
immunogen = synthetic peptide from the C-terminal of rat conjugated to BSA
VAChT both in CNS and PNS
-
VAT
Gentaur
polyclonal
IgG
Chicken
(neat serum, azide, available affinity purified)
-
Mouse, Rat
immunogen = twenty aa. peptide mapping within the C-terminus of rat/mouse VAT (75% homology with human VAT)
VAT; antibody labels cholinergic cell bodies in the septum and nucleus basalis and cholinergic fiber
-
VAChT
Valeant Pharmaceutical International
polyclonal
Rabbit
-
Human
VAChT
-
VAChT
Interchim
polyclonal
Goat
(antiserum, liquid, no preserve)
-
Mouse, Rat, Guinea Pig
immunogen = synthetic peptide corr. to aa 511-530 from cloned rat VAChT
VAChT; labeling of the interpeduncular nucleus of the brain stem andventral horn of the spinal cord
-
VAChT
Interchim
polyclonal
Chicken
(neat serum, azide, available affinity purified)
-
Mouse, Rat
immunogen = twenty aa. peptide mapping within the C-terminus of rat/mouse VAT (75% homology with human VAT)
VAT; antibody labels cholinergic cell bodies in the septum and nucleus basalis and cholinergic fiber
-
VAT
ISL (Immune Systems Limited)
polyclonal
Goat
(diluted, serum)
-
-
VAT
-
VAT
ISL (Immune Systems Limited)
polyclonal
Sheep
(Ig)
-
-
VAT
-
VAChT
PROGEN Biotechnik GmbH
polyclonal
Guinea Pig
(antiserum, lyophilized)
-
Mouse, Rat
immunogen = synthetic peptide from the C-terminus of rat VAChT conj. to BSA
VAChT in both CNS and PNS
-
VAChT
PROGEN Biotechnik GmbH
polyclonal
Rabbit
(antiserum, lyophilized)
-
Mouse, Rat
immunogen = synthetic peptide from the C-terminus of rat VAChT conj. to BSA
VAChT in both CNS and PNS
-
VAChT
PROGEN Biotechnik GmbH
polyclonal
Rabbit
(antiserum, lyophilized)
-
Human
immunogen = synthetic peptide from the C-terminus of human VAChT conj. to BSA
VAChT
-
VAChT
RDI /Fitzgerald
polyclonal
Guinea Pig
(antiserum, lyophilized)
-
Mouse, Rat
immunogen = synthetic peptide from the C-terminus of rat VAChT conj. to BSA
VAChT in both CNS and PNS
-
VAChT
RDI /Fitzgerald
polyclonal
Rabbit
(antiserum, lyophilized)
-
Mouse, Rat
immunogen = synthetic peptide from the C-terminus of rat VAChT conj. to BSA
VAChT in both CNS and PNS
-
VAChT
Sigma Aldrich
polyclonal
IgG
Rabbit
(affinity isolated, PBS, BSA, azide)
-
Human, Rat
immnuogen = synthetic peptide corr. to C-terminal of rat VAChT, conj. to KLH (aa 512-530, with N-terminally added lysine)
VAChT; epitope highly conserved (>85% homology) in human VAChT
-
Ubiquitin
Biogenesis AdB Serotec
polyclonal
Rabbit
(serum, lyophilized)
-
-
immunogen = purified bovine ubiquitin
ubiquitin
-
Ubiquitin
Biotrend
polyclonal
Rabbit
(purified IgG, PBS)
-
Cow, Human, Mouse, Rat
immunogen = ubiquitin from bovine erythrocytes
ubiquitin
-
Ubiquitin
Biotrend
polyclonal
Rabbit
(serum, lyophilized)
-
-
immunogen = purified bovine ubiquitin
ubiquitin
-
Ubiquitin (242.9)
Biotrend
monoclonal
IgG1
Mouse
(liquid, ascites, azide)
-
Human
immunogen = purified ubiquitin covalently coupled to KLH using glutaraldehyde
Ubiquitin
-
Ubiquitin (KM691)
Kamiya
monoclonal
IgM
Mouse
(liquid, ascites, azide)
-
Human
immunogen = recomb. human ubiquitin
ubiquitin
-
Ubiquitin+1
R&D Systems
polyclonal
IgG
Rabbit
(affinity purified, filtered, PBS, trehalose, lyophilized)
-
Human
immunogen = KLH coupled synthetic peptide CADLRED PDRQDHH PGSGAQ corr. to the C-terminus of Ubiquitin+1
rhUbiquitin+1
-
Ubiquitin+1 (83406)
R&D Systems
monoclonal
IgG2B
Mouse
(IgG fract, protein-G, PBS, filtered, lyophilized)
-
Human
immunogen = recombinant Ubiquitin+1 containing a His10 tag at the amino terminal
Ubiquitin and Ubiquitin+1
-
Ubiquitin (P1A6)
RDI /Fitzgerald
monoclonal
IgG1
Mouse
-
Human, Mouse, Rat, Various species
aa 1-46 of the amino terminus of ubiquitinof bovine origin
ubiquitin, poly-ubiquitin and ubiquitinated proteins
-
Ubiquitin Activating Enzyme (2G2.3-5)
Sigma Aldrich
monoclonal
IgG1
Mouse
(liquid, supernate, purified, PBS, azide)
-
Human, Mouse, Rabbit
immunogen = human and mouse Ubiquitin Activating Enzyme
E1 (Ubiquitin Activating Enzyme)
-
Ubiquitin (6C1)
Sigma Aldrich
monoclonal
IgG2a
Mouse
(liquid, ascites, azide)
-
Human, Mouse, Rat, Bovine
immunogen = purified bovine ubiquitin conj. to KLH
ubiquitin
-
Ubiquitin
Stressgen Enzo Life Sciences
polyclonal
Rabbit
(whole serum)
-
Sheep, Human, Monkey, Hamster, Mouse, Rat, Drosophila, Fish (various), Bovine, Dog, Rabbit, Chicken, Frog, E. Coli, Yeast
immunogen = ubiquitin purified from bovine red blood cells, conj. to KLH
free ubiquitin and ubiquinated proteins; will immuno- precipitate BSA-conjugated ubiquitin
-
Ubiquitin (FK2)
Stressgen Enzo Life Sciences
monoclonal
IgG1
Mouse
(protein-A, Ig, PBS, glycerol)
-
-
immunogen = crude poly ubiquitin lysozyme
ubiquitinated proteins; not free ubiquitin
-
Ubiquitin (UBI-1)
Zymed/ Invitrogen
monoclonal
IgG1 κ
Mouse
(liquid, PBS, azide)
-
Human, Mouse, C. elegans, Drosophila, Bovine, Chicken
immunogen = purified bovine ubiquitin conj. to carrier protein
ubiquitin, both conjugated and unconjugated; reacts with single chain 8.5 kDa protein
-
P53
R&D Systems
polyclonal
IgG
Goat
(affinity purified, IgG fract, PBS, trehalose, lyophilized)
-
Human, Mouse, Rat
immunogen = purified, E.Coli -derived recombinant human p53
p53
-
p53 (Pab1801)
Biosource Invitrogen
monoclonal
IgG1
Mouse
(ascites, purified IgG, PBS, BSA, azide, unconj.)
-
Human
immunogen = recombinant human wild type p53 protein expressed in E. coli(epitope within the N-terminus, aa 32-79, of p53)
mutant as well as wild type forms of p53
-
p53 (DO-7)
Biosource Invitrogen
monoclonal
IgG2b
Mouse
(ascites, purified IgG, PBS, BSA, azide, unconj.)
-
Cow, Human, Monkey
immunogen = recombinant human wild type p53 protein expressed in E. coli(epitope within the N-terminus, aa 37-45, of p53)
mutant as well as wild type forms of human p53
-
p53 (DO-7)
Biosource Invitrogen
monoclonal
IgG2b
Mouse
(purified ascites, PBS, BSA, azide, FITC conj.)
-
Cow, Human, Monkey
immunogen = recombinant human wild type p53 protein expressed in E. coli(epitope within the N-terminus, aa 37-45, of p53)
mutant as well as wild type forms of human p53
-
p53 (Pab240)
Biosource Invitrogen
monoclonal
IgG1
Mouse
(ascites, purified IgG, PBS, BSA, azide, unconj.)
-
Cow, Pig, Human, Monkey, Hamster, Mouse, Rat, Chicken
immunogen = Murine p53-β-galactosidase fusion protein (epitope maps within the middle region, aa 212-217, of p53)
reacts with only certain mutant p53 protein under non-denaturing condition in IP, but reacts with both mutant and wild form of p53 under denaturing condition in WB
-
p53 (Pab246)
Biosource Invitrogen
monoclonal
IgG2b
Mouse
(purified IgG, PBS, BSA, azide, unconj.)
-
Hamster, Mouse, Rat
immunogen = SV40 transformed cell line BALB/c SVA31 E7 (epitope between amino acid residues 88-93 of p53)
wildtype form of mouse p53, but not the mutant forms
-
p53 (Pab122)
Biosource Invitrogen
monoclonal
IgG2b
Mouse
(ascites, purified IgG, PBS, BSA, azide, unconj.)
-
Human, Monkey, Hamster, Mouse, Rat
immunogen = SV40-transformed mouse B4 cells (epitope maps near the C-terminus, aa 370-378, of p53)
mutant as well as wild type forms of p53
-
p53 Protein (DO-1)
Biosource Invitrogen
monoclonal
IgG2b
Mouse
(purified IgG, PBS, BSA, azide, unconj.)
-
Cow, Human, Monkey, Mouse, Rat
immunogen = ecombinant human wild type p53 protein expressed in E. coli(epitope within the N-terminus, aa 11-25, of p53)
mutant as well as wild type forms of human p53
-
p53 pSer392
Biosource Invitrogen
polyclonal
Rabbit
(PBS, carrier and preservative free, unconj.)
-
Human, Mouse, Rat
immunogen = sequence corr. to the mouse p53 protein surrounding serine 389 (epitope maps within the middle region, aa 212-217, of p53)
mouse p53 phosphorylated at serine position 389 andrecombinant and native human p53 phosphorylated at serine position 392.
-
p53 (246)
Kamiya
monoclonal
IgG1
Mouse
(ascites, protein-G, PBS, BSA, azide)
-
Hamster, Mouse, Rat
immunogen = SV40-transformed cell line BALB/c SVA31 E7 (epitope mapping between amino acids 88-93 of mouse wild-type p53)
wild-type p53; does not bind mutant p53.
-
p53 (BP53-12)
Kamiya
monoclonal
IgG2a
Mouse
(ascites, purified, PBS, BSA, azide)
-
Human
immunogen = recombinant human wild type p53 protein (epitope maps within the N-terminus, aa 20-25)
mutant and wild-type p53 under denaturing and non-denaturing conditions
-
p53
Kamiya
polyclonal
IgG
Sheep
(purified, protein-G, PBS, azide)
-
Human, Mouse, Rat
immunogen = GST p53 fusion protein containing amino acids 1-393 of human p53
wild-type and mutant p53.
-
p53 (BP53-12)
Kamiya
monoclonal
IgG2b ,κ
Mouse
(ascites, protein-A, PBS, BSA, azide)
-
Human, Monkey, Hamster, Mouse, Rat
immunogen = SV40-transformed mouse B4 cells (epitope mapping near the C-terminal end, aa 370-378)
conserved determinant of the p53 oncoprotein. immunoprecipitates the p53 oncoprotein complexed with the SV40 large T-antigen, the most ATPase-active form of the T antigen
-
P53 Lys382
R&D Systems
polyclonal
IgG
Rabbit
(affinity purified, PBS, glycerol)
-
Human
immunogen = peptide corr. to aa 377 - 387 of thehuman p53 sequence with an acetylated lysine at position 382
human p53 acetylated at lysine 382
-
P53 Lys382
R&D Systems
polyclonal
IgG
Rabbit
(affinity purified, PBS, glycerol)
-
Human, Mouse
immunogen = peptide corr. to aa 310 - 322 of the mouse p53 sequence with an acetylated lysine at position 317
p53 acetylated at lysine 317
-
P53 phos S15
R&D Systems
polyclonal
IgG
Rabbit
(affinity purified, concentrated, PBS, trehalose, lyophilized)
-
Human, Monkey, Mouse, Rat
immunogen = synthetic phospho peptide containing phospho serine at position 15 ofhuman p53
hp53 phosphorylated at S15 and mouse p53 phos. at S18; not unphos. p53
-
P53 phos S46
R&D Systems
polyclonal
IgG
Rabbit
(affinity purified, concentrated, PBS, trehalose, lyophilized)
-
Human
immunogen = synthetic phospho peptide containing phospho serine at position 46 ofhuman p53
hp53 phosphorylated at S46; not unphos. p53
-
p53 (BP53-12)
RDI /Fitzgerald
monoclonal
IgG2a
Mouse
(supernat, protein-A purified, PBS, BSA, azide; also biotin or FITC conj)
-
Human
epitope at aa 20-25
53 kDa nuclear protein, Wild-type and mutant
-
p53 (DO-12)
RDI /Fitzgerald
monoclonal
IgG2b
Mouse
(ascitic fluid, protein-A purified, PBS, BSA, azide)
-
Human, Mouse
epitope between aa 250 and 270, which is located between conserved domain IV and V on an exposed turn of the protein structure
reacts with human p53 protein, wild type and mutant forms under denaturing conditions; reacts with mutant form under non-denaturing conditions but not with wild-type
-
p53 (DO-14)
RDI /Fitzgerald
monoclonal
IgG1
Mouse
(ascitic fluid, protein-A purified, PBS, BSA, azide)
-
Human
epitope in aa 56 to 65, which is located the N-terminus region
reacts with human p53 protein, wild type and mutant forms under denaturing and non-denaturing conditions; in WB, recognizes an additional 40 kDa band to the 53 kDa band expressed by the HOS cell line
-
p53 (pAb 122)
RDI /Fitzgerald
monoclonal
IgG2b
Mouse
(supernat, purified, protein-A purified, PBS, BSA, azide; also biotin or FITC conj)
-
Human, Monkey, Hamster, Mouse, Rat
epitope at aa 370-378 on the C terminus of mouse p53
reacts with a conserved, denaturation- resistant determinant of the p53 protein
-
p53 (Pab 1801)
RDI /Fitzgerald
monoclonal
IgG1
Mouse
(supernat, protein-A purified, PBS, BSA, azide; also biotin or FITC conj)
-
Human
epitope within aa 32-79
human p53, wild-type and mutant forms
-
p53 (Pab240)
RDI /Fitzgerald
monoclonal
IgG1
Mouse
(supernat, protein-A purified, PBS, BSA, azide; also biotin or FITC conj)
-
Cow, Human, Monkey, Hamster, Mouse, Rat, Chicken
epitope at aa 213-217
50 kDa human p53
-
p53 (Pab246)
RDI /Fitzgerald
monoclonal
IgG1
Mouse
(supernat, protein-A purified, PBS, BSA, azide; also biotin or FITC conj)
-
Mouse
epitope at aa 85-109
50 kDa p53, wild type only
-
p53, pan (Pab 122)
Roche Applied Science
monoclonal
IgG2b
Mouse
-
Human, Hamster, Mouse, Rat
conserved, denaturation stable antigenic determinant of the p53 protein; shows strong nuclear staining on SV40- or BK virus-transformed cell lines and different tumor cell lines
-
p53, pan
Roche Applied Science
polyclonal
IgG
Sheep
(IgG, purified, lyophilized)
-
Cow, Human, Monkey, Hamster, Mouse, Rat, Chicken
immunogen = human recombinant p53, expressed in E.coli
wildtype and mutant p53; multiple epitopes, especially at the N-terminal and C-terminal regions of the p53 protein
-
p53, pan- peroxidase kit
Roche Applied Science
polyclonal
Sheep
-
Human, Mouse, Rat
detects wild-type and mutant p53 (pantropic capture antibody and polyclonal detection antibody)
-
p53 (B53.12)
Zymed/ Invitrogen
monoclonal
IgG2a κ
Mouse
(liquid, ascites,PBS, BSA, azide, blocking agent, 2nd Gen. predilute)
-
Human
p53
-
p53 (BP53.12)
Zymed/ Invitrogen
monoclonal
IgG2a ,κ
Mouse
(ascites, purified, PBS, BSA, azide)
-
Human
immunogen = recombinant human p53 (epitope between amino acids 1 and 45 in the p53 protein)
p53, both mutant and wild type
-
p53 (DO-7)
Zymed/ Invitrogen
monoclonal
IgG2b
Mouse
(PBS, BSA, azide, concentrate)
-
Mouse
immunogen = recombinant wild type p53 peptide (epitope located in the N-terminus of p53)
mutant and wild-type human p53
-
p53 (Pab1801)
Zymed/ Invitrogen
monoclonal
IgG1
Mouse
(ascites, purified, PBS, azide)
-
Human
immunogen = recombinant p53 peptide (epitope near the N-terminal, between 32-79 aa in the human sequence)
mutant and wild-type human p53; reactivity with a 75kD cytoplasmic protein has been reported
-
p53 (Pab1801)
Zymed/ Invitrogen
monoclonal
IgG1 κ
Mouse
(ascites, purified, PBS, BSA, azide, predilute)
-
Human
immunogen = p53 beta-galactosidase fusion protein (epitope between amino acids 45 and 91 in the p53 protein)
p53, both mutant and wild type
-
p53 (Pab240)
Zymed/ Invitrogen
monoclonal
IgG1 κ
Mouse
(ascites, purified, PBS, azide)
-
Human, Mouse, Rat, Mammal (various)
immunogen = recombinant p53 peptide (epitope between the evolutionally conserved Box III and IVregions, approximately 161-220 aa in the human sequence)
mutant and wild-type human p53; reactivity with a 75kD cytoplasmic protein has been reported
-
p53AIP-1
Zymed/ Invitrogen
polyclonal
Rabbit
(affinity purified, PBS, azide)
-
Mouse
immunogen = synthetic peptide derived from the C-terminus of human p53AIP-1.
p53AIP-1
-