(Timothy LaVaute, Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD)
used in paper by LaVaute, 2001
Formulation: affinity purified using affinity chromatography columns made by coupling MAP antigen to cyanogens bromide activated Sepharose CL-4B
immunogen = peptides that correspond to C termini encoded by two alternative splice forms of mouse Dmt1, one by exon that contains a non-consensus IRE in the 3’ UTR (C-terminus-K L L S E D T S G G N I K) and the other by an exon that lacks an IRE (C-terminus Q P E L Y L L N R T V D A D S V V S R)
- Lavaute T, Smith S, Cooperman S, Iwai K, Land W, Meyron-Holtz E, Drake SK, Miller G, Abu-Asab M, Tsokos M, Switzer R, Grinberg A, Love P, Tresser N, Rouault TA. Targeted deletion of the gene encoding iron regulatory protein-2 causes misregulation of iron metabolism and neurodegenerative disease in mice. Nat Genet. 2001 Feb;27(2):209-14. PubMed.
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