Antibody

FLAG (M2)

Clonality: monoclonal
Host: Mouse
Isotype: IgG1
Methods: DB, ELISA, WB
Source: Sigma Aldrich
Cat# A9469
References:
Wakabayashi, 2009
Serneels, 2009
Tsuda, 2008
Osawa, 2008
Sato, 2007
(all, unspecified formulation)
Formulation: purified Ig, buffered aqueous glycerol solution, TBS, glycerol, stabilizer, preservative, AP conj.<br />also available as HRP conj., <a href="http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A8592" target="_new">A8592</a> or biotin labeled, <a href="http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/datasheet2/f9291dat.Par.0001.File.tmp/f9291dat.pdf" target="_new">F9291</a>
Reactivity:
Reacts in: Various species, Mouse, Rat

Specificity

Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent; useful in detection of FLAG fusion proteins expressed in murine host

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References

Paper Citations

  1. . Analysis of the gamma-secretase interactome and validation of its association with tetraspanin-enriched microdomains. Nat Cell Biol. 2009 Nov;11(11):1340-6. PubMed.
  2. . gamma-Secretase heterogeneity in the Aph1 subunit: relevance for Alzheimer's disease. Science. 2009 May 1;324(5927):639-42. Epub 2009 Mar 19 PubMed.
  3. . The amyotrophic lateral sclerosis 8 protein VAPB is cleaved, secreted, and acts as a ligand for Eph receptors. Cell. 2008 Jun 13;133(6):963-77. PubMed.
  4. . Phosphoinositides suppress gamma-secretase in both the detergent-soluble and -insoluble states. J Biol Chem. 2008 Jul 11;283(28):19283-92. PubMed.
  5. . Active gamma-secretase complexes contain only one of each component. J Biol Chem. 2007 Nov 23;282(47):33985-93. PubMed.

External Citations

  1. A8592
  2. F9291

Further Reading

No Available Further Reading