Antibody

FLAG

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Clonality: polyclonal
Host: Rabbit
Isotype: IgG
Source: Sigma Aldrich
Cat# F7425
References:
Li H, 2010
Maruyama, H, 2010
He, 2009
Formulation: affinity purified, buffered aqueous solution, IgG, PBS, BSA, azide
Reactivity:
Reacts in: Non-specific

Immunogen/Epitope

immunogen = Synthetic FLAG sequence containing peptide DYKDDDDK-GC conjugated through a terminal cysteine thiol to maleimide-activated KLH

Specificity

recognizes the FLAG epitope located on FLAG fusion proteins; reacts with Nterminal, N-terminal-Met, and C-terminal FLAG fusion proteins

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References

Paper Citations

  1. Li H, Wang B, Wang Z, Guo Q, Tabuchi K, Hammer RE, Südhof TC, Zheng H. Soluble amyloid precursor protein (APP) regulates transthyretin and Klotho gene expression without rescuing the essential function of APP. Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17362-7. PubMed.
  2. Maruyama H, Morino H, Ito H, Izumi Y, Kato H, Watanabe Y, Kinoshita Y, Kamada M, Nodera H, Suzuki H, Komure O, Matsuura S, Kobatake K, Morimoto N, Abe K, Suzuki N, Aoki M, Kawata A, Hirai T, Kato T, Ogasawara K, Hirano A, Takumi T, Kusaka H, Hagiwara K, Kaji R, Kawakami H. Mutations of optineurin in amyotrophic lateral sclerosis. Nature. 2010 May 13;465(7295):223-6. PubMed.
  3. He P, Shen Y. Interruption of beta-catenin signaling reduces neurogenesis in Alzheimer's disease. J Neurosci. 2009 May 20;29(20):6545-57. PubMed.

Further Reading

No Available Further Reading